| --- Log opened Tue Jun 12 00:00:38 2012 | ||
| archels | fenn: haha, wonderful. | 00:18 |
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| @kanzure | diy biojihad | 08:26 |
| Mariu | =] | 08:33 |
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| @kanzure | klafka: why aren't you here? | 08:45 |
| @kanzure | walnut creek marriot.. floor b2 | 08:45 |
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| delinquentme | http://www.sciencedirect.com/science/article/pii/0168165693901235?via=ihub | 09:01 |
| delinquentme | kanzure, what did you do | 09:01 |
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| gnusha_ | diyhpluswiki.git: f19d4c4 intro from nathan head | 09:18 |
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| gnusha_ | diyhpluswiki.git: ce7d6fa jojack talk | 09:43 |
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| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/intro.txt | 09:46 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/joseph-jackson.txt | 09:46 |
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| @kanzure | Juul: HI | 10:04 |
| Juul | kanzure, :) | 10:04 |
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| nmz787_ | 10X!!!!!! Whooooo! | 10:15 |
| Tukotih | No way, you guys have a website? | 10:18 |
| Tukotih | Lol, have you written that Wiki yourself? In PHP? | 10:19 |
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| delinquentme | nmz787_, 10x? | 10:24 |
| nmz787_ | Someone said 10x reduction in dna synth using pooled arrays | 10:25 |
| nmz787_ | In price* | 10:25 |
| delinquentme | what is a pooled array? | 10:28 |
| nmz787_ | Take a microarray and dissolve all the oligos then ligate... IMO it isnt the way to go | 10:33 |
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| delinquentme | "Home made electroporation apparatus" http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=8&sqi=2&ved=0CKIBEBYwBw&url=http%3A%2F%2Fwww.biosci.missouri.edu%2Fsmithgp%2FPhageDisplayWebsite%2Felectroporator.doc&ei=fIDXT-HqMKT46QHzv5C7Aw&usg=AFQjCNHbV0PgscqGzxtmFzMMFcfigiF7hw | 10:47 |
| Lukas | thanks | 10:48 |
| Juul | delinquentme, cool | 10:48 |
| delinquentme | ^ | 10:48 |
| delinquentme | is there like an exceedingly simple process to test out electroporation on yeast anyone knows of? | 10:53 |
| delinquentme | like GFP yeast but with something cheaper / more readily available than GFP? | 10:53 |
| Juul | a amp/kan resistance plasmid? | 10:56 |
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| gnusha_ | diyhpluswiki.git: e467e52 security issues session | 10:56 |
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| nmz787_ | Gfp is just as cheap as amp/kan plasmid | 10:57 |
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| Juul | kanzure, i fixed some typos but don't have push access :-/ | 11:06 |
| Juul | what is the easiest way for me to send you fixes? | 11:09 |
| Juul | who's the guy talking right now? | 11:11 |
| Juul | oh Nathan | 11:12 |
| Juul | :) | 11:12 |
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| gnusha_ | diyhpluswiki.git: ad00740 security issues question session | 11:32 |
| @kanzure | juul: github i guess. also you can hand me your public key and i can add you for git pushin'. | 11:34 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/security-issues-session.txt | 11:36 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/meg-flanagan-department-of-state.txt | 11:36 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/piers-millett.txt | 11:36 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/todd-kuiken-woodrow-wilson.txt | 11:36 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/chris-anderson-syncberc.txt | 11:36 |
| @kanzure | Tukotih: it's a git wiki | 11:37 |
| @kanzure | nmz787_: yeh i'm surprised chris was pimping dna microarrays. how lame. | 11:37 |
| archels | Did you type those up live? | 11:37 |
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| @kanzure | archels: yes | 11:44 |
| archels | nice :D | 11:45 |
| gnusha_ | diyhpluswiki.git: a45b5c2 where to send receipts | 11:45 |
| nmz787_ | Kanzure maybe the rreceipts stuff shouldnt be online | 11:46 |
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| @kanzure | beep boop | 13:12 |
| Jora | beeeeep! | 13:12 |
| Tukotih | is gnusha_ a bot? | 13:19 |
| Tukotih | Just curious | 13:20 |
| @kanzure | Tukotih: yes | 13:22 |
| chris_99 | anyone used sqlalchemy before? | 13:23 |
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| mensch | chris_99: yes | 13:48 |
| chris_99 | mensch, do you mind having a quick look at some code | 13:48 |
| mensch | sure | 13:49 |
| chris_99 | cheers i'll just pastebin it | 13:49 |
| mensch | no worries | 13:49 |
| chris_99 | mind if i pm you | 13:50 |
| mensch | That's okay | 13:51 |
| gnusha_ | diyhpluswiki.git: d40c4fa first part of the innovation block | 13:53 |
| strangewarp | I'd just like to note that I despise consent-reality fundamentalists | 13:58 |
| strangewarp | you argue objectivity, and they're all "hup ho, but objectivity is just what we all agree on!" no it fucking isn't | 13:58 |
| gnusha_ | diyhpluswiki.git: 0502798 mac's talk thing | 13:59 |
| _Sketch_ | strangewarp: amen | 14:04 |
| _Sketch_ | I once had someone arguing on how you could have synesthesia if you believed hard enough. | 14:05 |
| _Sketch_ | I failed to impress upon them that that isn't how synesthesia works. | 14:06 |
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| gnusha_ | diyhpluswiki.git: 706bf17 chicago talk | 14:08 |
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| gnusha_ | diyhpluswiki.git: 4e78480 biocurious | 14:48 |
| gnusha_ | diyhpluswiki.git: 5aebc8f ellen jorgensen / genspace | 14:48 |
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| gnusha_ | diyhpluswiki.git: 416c696 derek jacoby | 14:58 |
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| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/carlo-quinonez.txt | 15:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/chicago-open-science.txt | 15:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/derek-jacoby.txt | 15:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/kristina-hathaway-biocurious.txt | 15:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/ellen-jorgensen-genspace.txt | 15:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/mac-cowell.txt | 15:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/nancy-burgess-dtra.txt | 15:03 |
| @kanzure | chris_99: yes i've used sqlalchemy. also try quick_orm or something. | 15:04 |
| Tukotih | What are those for? | 15:04 |
| Tukotih | "Object Relational Mappers" | 15:05 |
| Tukotih | Like, social networking, and? | 15:05 |
| @kanzure | _Sketch_: one of the things lots of us in ##hplusroadmap want is a way to induce synthesia (and the shroom stuff doesn't entirely count) | 15:05 |
| @kanzure | Tukotih: hahah no. definitely not. | 15:05 |
| chris_99 | managed to get it working pretty well now :) was a bit of a learning curve after JPA | 15:05 |
| @kanzure | Tukotih: ORM is for writing software | 15:06 |
| @kanzure | things like datamapper, activerecord, sqlalchemy | 15:06 |
| Tukotih | kanzure: I'm a newbie to dbs, only know a bit of SQL. | 15:06 |
| Tukotih | Huh | 15:06 |
| Tukotih | I need to check it out | 15:06 |
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| @kanzure | you use ORMs instead of writing queries directly | 15:06 |
| Tukotih | Aaaaah | 15:06 |
| @kanzure | most of the usual queries that you use in a typical web app or project are easily optimized by these libraries | 15:07 |
| chris_99 | it makes creating the db a lot easier imo | 15:07 |
| Tukotih | Cool | 15:07 |
| @kanzure | in extreme situations (like maybe you're fucking facebook) you need to write optimized queries by hand, but the chances are very high that you sir are not facebook | 15:07 |
| Tukotih | Are they compatible with GAE? | 15:07 |
| @kanzure | yes people use orms on gae | 15:07 |
| Tukotih | Not that I'm going to use it, was just the first one I really learned. | 15:08 |
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| nmz787_ | Im a bit bored | 15:18 |
| gnusha_ | diyhpluswiki.git: 9d84dfb avery at bosslab | 15:21 |
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| gnusha_ | diyhpluswiki.git: 74329d3 madlab/uk | 15:38 |
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| gnusha_ | diyhpluswiki.git: 5fe10e3 pieter van boheemen | 15:50 |
| gnusha_ | diyhpluswiki.git: 948dd77 oops, missed some questions | 15:50 |
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| gnusha_ | diyhpluswiki.git: e8aca96 la paillasse | 16:14 |
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| gnusha_ | diyhpluswiki.git: 93ab0f4 genoblasts in baltimore | 16:28 |
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| gnusha_ | diyhpluswiki.git: af64938 denisa kera talk | 16:40 |
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| gnusha_ | diyhpluswiki.git: f55b55e chido talk | 16:50 |
| -!- mode/##hplusroadmap [-b *!*Omega@started.the.rvlution.net] by kanzure | 16:54 | |
| @kanzure | oh wait | 16:54 |
| @kanzure | yeah, -b. ok. | 16:55 |
| gnusha_ | diyhpluswiki.git: 45bfb8e natural-fiber.com i think | 16:59 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/avery-louie-bosslab.txt | 17:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/denisa-kera.txt | 17:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/genoblasts.txt | 17:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/madlab.txt | 17:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/micronation-natural-fiber.txt | 17:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/pieter-van-boheemen.txt | 17:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/radka-chido-haneckova.txt | 17:03 |
| @kanzure | http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/thomas-landrain-la-paillasse.txt | 17:03 |
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| @kanzure | fenn: would it be a terrible tragedy if i showed up one day early to langton? | 17:08 |
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| @kanzure | hi SilenceFromMars | 17:19 |
| ParahSailin | whyd you guys talk to the fbi | 17:20 |
| -!- |G|00M| is now known as Dr_Patrick | 17:21 | |
| nmz787_ | Fbi put on a workshop for free, so we told them whats up | 17:23 |
| nmz787_ | Also i like being part of the relationship that should help keep bio labs safe from meth lab related scares | 17:24 |
| ParahSailin | didnt you learn in civics class to never talk to cops or volunteer information to leos in any way? | 17:25 |
| ParahSailin | http://www.youtube.com/watch?v=6wXkI4t7nuc | 17:28 |
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| SilenceFromMars | this guy is Saul Goodman in real life | 17:38 |
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| @kanzure | ParahSailin: no i missed that class | 19:58 |
| @kanzure | eh .. http://diybio.org/2012/06/12/hardware-add-a-project/ | 20:06 |
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| @kanzure | also: apparently charles babbage wasn't the machinist who made his machine | 20:31 |
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| gnusha_ | diyhpluswiki.git: 9a7dfef fixes from john griessen | 20:34 |
| gnusha_ | diyhpluswiki.git: c52f5ab more fixes from john griessen | 20:34 |
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| klafka1 | i am having such issues getting hadoop running | 20:35 |
| klafka1 | anyone use it? | 20:35 |
| @kanzure | hrmm, i haven't specifically used that yet :( | 20:36 |
| @kanzure | also why haven't you showed up yet | 20:36 |
| klafka1 | where am i showing up to? | 20:37 |
| klafka1 | sorry | 20:37 |
| klafka1 | i've been so out of it | 20:37 |
| klafka1 | are you in california now? | 20:37 |
| @kanzure | sf | 20:37 |
| @kanzure | yes | 20:37 |
| @kanzure | diybio/fbi thing | 20:37 |
| klafka1 | is that this weekend? | 20:37 |
| @kanzure | tomorrow. 8am-8pm ish. | 20:37 |
| klafka1 | tomorrow | 20:37 |
| @kanzure | no this weekend is a different thing that you should go to | 20:37 |
| klafka1 | where ? | 20:37 |
| @kanzure | http://startupscience.eventbrite.com/ | 20:37 |
| @kanzure | tomorrow's thing is at the walnut creek marriott | 20:37 |
| @kanzure | mariott | 20:38 |
| sylph_mako | I hear Babbage poured funding into precise machining as a prerequisite project. England became a world leader in precise machinery, but the difference engine was never made. | 20:38 |
| klafka1 | umm | 20:38 |
| klafka1 | there is no way i can get there by 8pm if it's in walnut creek | 20:38 |
| @kanzure | sylph_mako: i thougth the difference engine /was/ made | 20:38 |
| klafka1 | my job is being ridiculous atm | 20:38 |
| sylph_mako | Not in a useful way. | 20:39 |
| klafka1 | i'm ardently searching for a new one | 20:39 |
| @kanzure | klafka1: well, the 8am session wont start until 830 am probably | 20:39 |
| sylph_mako | Was it? | 20:39 |
| sylph_mako | Like did anyone ever actually use one? | 20:39 |
| @kanzure | not sure. i'm very confused about this. | 20:39 |
| @kanzure | so like, the guy he hired, did all the screw standards? | 20:39 |
| @kanzure | fenn: you would probably know this? | 20:39 |
| sylph_mako | I don't know the details, I'm not even sure how strong the causal link between his investment into fine machinery and england's capacity for it is. | 20:40 |
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| --- Log closed Wed Jun 13 00:00:39 2012 | ||
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