| --- Log opened Tue Jul 03 00:00:57 2012 | ||
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| Utopiah | http://metabiological.wordpress.com/2012/07/01/rapamycin-potential-cognitive-enhancer/ | 02:50 |
|---|---|---|
| AdrianG | -.- | 02:52 |
| nsh | cognition isn't that simple. | 02:57 |
| nsh | then again, there's DMT... | 02:58 |
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| chido | http://www.dailymail.co.uk/news/article-43767/Worlds-GM-babies-born.html | 03:58 |
| chido | "Writing in the journal Human Reproduction, the researchers, led by fertility pioneer Professor Jacques Cohen, say that this 'is the first case of human germline genetic modification resulting in normal healthy children'." | 03:58 |
| chido | I need that article, but I can't seem to be able to find it anywhere... any help appreciated | 03:59 |
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| nsh | that quote's from an article in the same journal in the year 2000 | 04:24 |
| nsh | http://humrep.oxfordjournals.org/content/16/3/513.full | 04:25 |
| nsh | (moral: don't trust tabloids to reference properly) | 04:27 |
| nsh | read: http://www.lifenews.com/2012/07/02/worlds-first-genetically-modified-babies-born-or-were-they/ | 04:28 |
| chido | thanks | 04:28 |
| nsh | np | 04:29 |
| chido | I thought it was a bit odd | 04:30 |
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| @kanzure | "The standard model gives very specific predictions for the behavior of the Higgs, which are fixed by the distinctive properties of the Higgs field (spontaneous breaking of the SU(2) gauge symmetry fixes how it interacts with gauge fields, giving mass to fermions fixes its interaction with them)." | 06:43 |
| @kanzure | "These predictions have for a long long time shown that if the Higgs mass is 125 GeV, it could not be observed until the energies and luminosities of the LHC were available, and then it should be seen with certain specific signal sizes in certain specific decay channels." | 06:43 |
| @kanzure | "What the LHC experiments are seeing is, after decades of no such signal in these and similar channels, signals appearing in just the right channels, with roughly the right signal size. If this isn’t a Higgs, it’s something very like it." | 06:43 |
| @kanzure | ^ http://www.math.columbia.edu/~woit/wordpress/?p=4809&cpage=1#comment-115426 | 06:43 |
| @kanzure | here's some ok coverage: http://www.theatlanticwire.com/global/2012/07/best-physics-gossips-you-should-be-reading/54123/ | 06:44 |
| @kanzure | http://blog.vixra.org/2012/06/24/higgs-discovery-on-the-brink-but-is-it-the-higgs/ | 06:45 |
| @kanzure | http://blog.vixra.org/2012/06/29/whats-the-deal-with-h-%E2%86%92-ww/ | 06:45 |
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| @kanzure | delinquentme: i responded to your skype request but you seem to be dead | 07:52 |
| audy | Are there any DNA oligomer synthesis companies with an API? | 07:53 |
| audy | I want to make a GET request and receive a fragment in the mail | 07:53 |
| @kanzure | none that i know of | 07:56 |
| @kanzure | i'm not sure that would be anything other than a novelty at this point | 07:56 |
| audy | life biosciences lets you upload a .csv file. I guess that could be automated | 07:56 |
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| @kanzure | ouch, csv | 07:58 |
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| @kanzure | i don't see how an HTTP API would increase the utility of said service though | 07:59 |
| @kanzure | like.. what sort of software would use that ? | 07:59 |
| audy | kanzure I need to order 700 primers | 07:59 |
| @kanzure | ah okay | 07:59 |
| audy | and I'm in a small lab | 07:59 |
| @kanzure | wait, why do you need 700 different primers? | 07:59 |
| audy | kanzure each one has a unique 7-mer barcode that allows me to pool DNA from many samples into the same lane of Illumina | 08:00 |
| audy | kanzure you have to optimize for cost of sequencing vs. cost of primers | 08:01 |
| @kanzure | i still don't understand. you're using all these primers with some reaction to separate fragments into lanes? | 08:02 |
| audy | kanzure I'm amplifying bacterial 16S rRNA from many samples. The library for each sample is made with a unique barcode. I then pool the samples and sequence them on the same lane | 08:02 |
| audy | kanzure Later, I separate the reads by barcode | 08:03 |
| @kanzure | okay. | 08:03 |
| @kanzure | so the 700 primers is because you don't have a library of these primers/barcodes already | 08:03 |
| @kanzure | *physical library | 08:03 |
| audy | right | 08:03 |
| @kanzure | so someone gave you a library without the primers? | 08:04 |
| @kanzure | or maybe you just want to select 700 out of N of the ones you are interested in? | 08:04 |
| audy | I'm not sure I understand | 08:04 |
| audy | I need to order 700 primers, each one has a unique 7-bp sequence. The rest of the primer is the same | 08:04 |
| @kanzure | ok, so you're saying that these 700 primers are your library, or that you have an existing bottle with some fragment library, that you apply against the rRNA samples? | 08:05 |
| audy | kanzure yes the second one | 08:05 |
| audy | kanzure I'm building the library using these primers and environmental DNA | 08:05 |
| @kanzure | i see. | 08:05 |
| @kanzure | so i think that would be the *first* one | 08:05 |
| @kanzure | o_o | 08:05 |
| audy | the library is the amplicon, no? | 08:06 |
| * audy never gets to actually hold a pipette | 08:06 | |
| @kanzure | hah | 08:06 |
| @kanzure | well library can mean anything, sure | 08:06 |
| @kanzure | it could be the end result or the stuff you start with | 08:06 |
| @kanzure | okay but anyway, i understand better now | 08:07 |
| @kanzure | i thought someone gave you a library of oligos, and you were making primers to select specific oligos, and then you do full strand hybridization against the rRNA molecules | 08:07 |
| @kanzure | but what you're actually doing is you just have rRNA fragments right now, and you want primers. | 08:07 |
| @kanzure | so yes i agree that a programmatic way to order this library would be nice :) | 08:08 |
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| audy | it might be useful for synth. bio also but I guess if one would just synthesize their own oligos at that point | 08:12 |
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| @kanzure | audy: a few years ago i was thinking about a synthetic dna bidding markt, where suppliers would bid for your order, but i couldn't figure out why they would want to do it :) | 08:15 |
| @kanzure | *market | 08:18 |
| audy | kanzure better yet, one where people can bid on the oligos themselves | 08:18 |
| @kanzure | i don't have a good feel or how much daily unused capacity there is on dna synthesizers | 08:20 |
| @kanzure | but obviously companies want zero unused capacity | 08:20 |
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| @kanzure | academentia | 11:10 |
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| nmz787 | scrapification http://gnusha.org/~nmz787/chemistry.gravitywaves.com/logs/ | 11:36 |
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| @kanzure | hrmm | 13:57 |
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| @kanzure | urgh http://www.hackpittsburgh.org/biohacking-and-grinder-culture-artsy-fartsy-you-bet | 15:43 |
| @kanzure | electrode manipulator http://www.backyardbrains.com/micromanipulator.aspx | 15:46 |
| @kanzure | "Commercially available manipulators typically cost $500-$1000 and are made of high quality steel. Using a MakerBot Thing-O-Matic and Replicator, we have designed a manipulator, code named: The Searcher (see below), made of tough ABS plastic, that is now for sale!" | 15:46 |
| @kanzure | "Notably, we have also used our Searcher to record electrical responses from muscle tissue in optogenetic fruit fly larva (larva with special ion channels in their neurons that are sensitive to blue light)." | 15:46 |
| @kanzure | eww .stl files | 15:46 |
| @kanzure | damn it | 15:46 |
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| Steel2 | kanzure, I have a new phrase for you to hate me over | 16:55 |
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| @kanzure | Steel2: uh oh | 17:22 |
| jmil | kanzure: what did u think of the sugar printing? | 17:24 |
| jmil | what would you like to see us do next? | 17:24 |
| Steel2 | h+omies | 17:31 |
| Steel2 | jmil, did you do the sugar printing? | 17:31 |
| jmil | Steel2: yes | 17:31 |
| Steel2 | fucking sweet | 17:31 |
| Steel2 | my thesis is in electrohydrodynamic inkjet micro/nanoprinting | 17:31 |
| jmil | Steel2: thanks! and awesome! where are u studying? | 17:32 |
| Steel2 | well, I'm out (I just have to defend now), but I was at RPI | 17:32 |
| Steel2 | I'll send you a copy of my thesis if any of it's relevant | 17:32 |
| eudoxia | hahaha h+omies | 17:32 |
| jmil | Steel2: awesometown. pls send to jsmiller@seas.upenn.edu i'd love to take a look | 17:33 |
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| Steel2 | hey thorb | 17:40 |
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| panax | should i do 23andme y/n? | 18:39 |
| yashgaroth | wait for the dna day sale...in 10 months | 18:40 |
| Steel2 | get chev to do it for you :P | 18:40 |
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| kanzure | where has everyone gone | 19:38 |
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| Steel2 | kanz, 4th celebrations? | 19:42 |
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| kanzure | Steel2: they are independent plans | 19:48 |
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| strangewarp | [18:31] <Steel2> h+omies | 20:18 |
| strangewarp | I will cut you | 20:18 |
| strangewarp | 0_0 | 20:18 |
| Steel2 | by the way, jmil | 20:19 |
| Steel2 | you are fucking awesome | 20:19 |
| Steel2 | though you should know that | 20:19 |
| yashgaroth | don't be such a h+ater strangewarp | 20:19 |
| strangewarp | oh god it's spreading | 20:19 |
| Steel2 | ch+ll out. | 20:19 |
| strangewarp | If this were a Crichton novel we'd all be dead in 30 minutes | 20:20 |
| Steel2 | jmil: I just used your article to hit on a girl on okcupid. | 20:20 |
| kanzure | don't be that dude | 20:21 |
| kanzure | don't be that guy | 20:21 |
| Steel2 | we were talking about organ replacements | 20:21 |
| Steel2 | it came up | 20:21 |
| Steel2 | she was happy | 20:21 |
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| chevbird | well....she is now | 20:30 |
| Steel2 | heh. | 20:30 |
| nmz787 | chido? | 20:33 |
| Steel2 | ? | 20:33 |
| chevbird | ? | 20:33 |
| yashgaroth | ? | 20:33 |
| nmz787 | ? | 20:34 |
| chevbird | go on.... | 20:34 |
| nmz787 | oh nevermind, i was reading the logs and i thought she needed an article | 20:34 |
| nmz787 | shit, i missed this http://www.hackpittsburgh.org/biohacking-and-grinder-culture-artsy-fartsy-you-bet | 20:38 |
| nmz787 | i coulda went home for a few days :/ | 20:38 |
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| foucist | strangewarp: which novel | 20:49 |
| foucist | i think i'd survive longer than 30 minutes in most of his novels ;) | 20:50 |
| * strangewarp shakes the Michael Crichton Novel Title Generator | 21:01 | |
| strangewarp | "Idiom Valley" | 21:01 |
| strangewarp | Where new memes gain intelligence and replace genes, turning people into mutants | 21:01 |
| strangewarp | movie rights immediately bought by Paramount, etc. | 21:02 |
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| foucist | sweet | 21:07 |
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| Juul | are there really no more Library Genesis mirrors up? | 21:15 |
| Juul | oh wait | 21:17 |
| Juul | there is | 21:17 |
| kanzure | why don't i have a mirror of that, again? | 21:19 |
| yashgaroth | link plz | 21:20 |
| Juul | kanzure, 8 terabytes? | 21:21 |
| Juul | http://libgen.info/ | 21:21 |
| kanzure | yeah 8 terabytes shouldn't be a problem | 21:21 |
| Juul | kanzure, here's what you need to create a mirror: https://thepiratebay.se/torrent/7191627/Library_Genesis_Repository_-_832K_eBooks_torrents__dB_and_source. | 21:21 |
| kanzure | yeah but last i checked it was only seeded at 5 kbps | 21:22 |
| kanzure | "seeders: 5" :( | 21:22 |
| Juul | but that's just the torrent of torrents | 21:22 |
| Juul | and the mysql db and php | 21:23 |
| Juul | source code is also here: http://www.assembla.com/code/libgen/subversion/nodes | 21:23 |
| Juul | mysql database is also here: http://dl.dropbox.com/u/3268251/backup-last.rar | 21:24 |
| kanzure | yes but are the actual torrents seeded? | 21:24 |
| Juul | not sure | 21:24 |
| chevbird | deep seeded | 21:24 |
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| Juul | hm I can't find anything that's seeded | 21:41 |
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| kanzure | yep.. same situation last year basically | 21:43 |
| kanzure | i sort of doubt that anyone has a copy | 21:43 |
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| Juul | wait | 21:45 |
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| Juul | tried the first one from this list: http://pastebin.com/MTwj0WeK | 21:45 |
| Juul | and for that one at least there's a seed that's maxing out my (crappy) connection | 21:46 |
| kanzure | jrayhawk: can we throw those into the queue? | 21:46 |
| jmil | Steel2: lol thanks man. best. post. evar. hope it works out! | 21:48 |
| jmil | kanzure: what would you like to see next from the sugar printing? | 21:52 |
| Juul | tried one of the middle-numbered torrents and one of the last and they also had seeders with decent speeds | 21:52 |
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| nmz787 | kanzure do you know anyone at makerbot? | 22:52 |
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| --- Log closed Wed Jul 04 00:00:58 2012 | ||
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