| --- Log opened Fri Sep 21 00:00:32 2012 | ||
| nmz787 | lol | 00:01 |
|---|---|---|
| @kanzure | but that's definitely a more long-term scheme | 00:05 |
| @kanzure | gotta be committed. | 00:05 |
| foucist | calories-in/calories-out for hoomans? | 00:05 |
| @kanzure | nsh: most people mean "calories out" in terms of "exercising" | 00:06 |
| foucist | i think there might be some papers that show people getting fat off high-carb low-calorie diets | 00:06 |
| @kanzure | but i'm pretty sure you poop more calories than you could ever exercise out | 00:06 |
| foucist | well, yeah there's the ole weighing poop and burning it | 00:07 |
| @kanzure | "mom's vintage poop burning machine" | 00:09 |
| nmz787 | can someone get this | 00:27 |
| nmz787 | http://www.ingentaconnect.com/content/iafp/jfp/1999/00000062/00000002/art00016?token=00501e2085cc62ff5f8c9a437a63736a6f3b47654c76766a24702a446f644a467b4d616d3f4e4b34 | 00:28 |
| @kanzure | i don't seem to have access | 00:35 |
| * kanzure sleeps | 00:35 | |
| nmz787 | :( | 00:36 |
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| skorket | morning | 07:58 |
| kirka | Hi | 08:23 |
| chris_99 | yo | 08:25 |
| * kirka reads kanzure's copy of "Nanosystems" | 08:31 | |
| kirka | Looks like kanzure has the only scanned copy in the net | 08:31 |
| kirka | I searched for it a lot and haven't found anything | 08:31 |
| kirka | I think I should torrent it (with kanzure's permission of course) | 08:32 |
| kirka | Nanosystems is a good book, expanded and corrected version of Drexler's thesis | 08:33 |
| kirka | That's it http://gnusha.org/stuff_to_deal_with/nanosystems.tar.gz | 08:34 |
| @kanzure | don't attribute any resulting torrents to me | 08:38 |
| kirka | Of course | 08:38 |
| kirka | Do you think that this book should have wider distribution? | 08:39 |
| @kanzure | i don't know what you mean | 08:39 |
| @kanzure | copenhagen suborbitals has released CAD files http://www.copenhagensuborbitals.com/resources_downloads.php | 08:39 |
| @kanzure | "Solidworks file for testing pressure hull of space capsule Tycho Deep Space-II" | 08:40 |
| @kanzure | "Solidworks and CAD files for a public assignment on the hatch design for Tycho Deep Space" | 08:40 |
| @kanzure | "Solidworks and STL files for the main construction of capsule Tycho Deep Space." | 08:40 |
| kirka | I mean that there are probably people who search for that book. Do you think I should upload it so they could find it? | 08:41 |
| Urchin | untill I saw the link I was happy enough with the thesis | 08:42 |
| kirka | It depends on your position over copyright, I agree | 08:42 |
| @kanzure | kirka: do you know the http://libgen.net/ guy? | 08:42 |
| Urchin | I'm more inclined to share this sort of thing on request only | 08:42 |
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| @kanzure | because you should add it to libgen | 08:42 |
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| kirka | kanzure Well, looks like mirror of that library runs in my nnetwork (Tiera) - bib.tiera.ru | 08:43 |
| kirka | kanzure Ok, I'll try | 08:43 |
| @kanzure | no tiera is different | 08:43 |
| @kanzure | heh "Download static list of all books: html - 18.59M, gzip - 3.8M" http://bib.tiera.ru/index.htm.gz | 08:44 |
| kirka | The source of books is the same | 08:44 |
| @kanzure | oh? | 08:44 |
| kirka | I'll try to do it with libgen | 08:44 |
| kirka | Ther is a forum | 08:44 |
| @kanzure | i know very little about tiera.ru.. where's the forum? | 08:44 |
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| kirka | No tiera.ru is a mirror | 08:50 |
| kirka | That;s it: http://genofond.org/viewtopic.php?f=8&t=6570 | 08:50 |
| kirka | You could just post them your gnusha link with description | 08:51 |
| @kanzure | i would rather not have it linked to gnusha. | 08:51 |
| kirka | Ok | 08:52 |
| kirka | Yes, that's rational | 08:52 |
| kirka | I can upload it to piratebay as anonymous and give them a link | 08:52 |
| kirka | I think Dr Drexler wouldn't mind if a dozen of interested people will read his 1992 book for free :) | 08:54 |
| @kanzure | don't link to my servers | 08:55 |
| kirka | Ok | 08:55 |
| kirka | Just a folder with tiff files, and description copied from the net | 08:56 |
| Urchin | for a dozen interested people torrents don't make much sense | 08:59 |
| kirka | Well I don't have piratebay account, don't you know some filesharing service that allows to upload 800 MB ? | 09:00 |
| kirka | I'll find it, don't mind | 09:04 |
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| eudoxia | kirka we should probably ocr it first | 09:30 |
| eudoxia | 700 mb of tiff files will scare many people away | 09:31 |
| kirka | eudoxia I tried already, but to no success | 09:31 |
| kirka | Hi | 09:31 |
| eudoxia | well, let's find the smallest jpeg that is decently readable | 09:31 |
| eudoxia | i think i already did that, when i put it on my kindle | 09:32 |
| kirka | btw Seems like you have written that article: http://wiki.transhumani.com/index.php?title=Molecular_Nanotechnology | 09:32 |
| kirka | It's interesting that information that I collected through search is the same. | 09:32 |
| eudoxia | oh yes here it is, 108 mb | 09:32 |
| eudoxia | I did | 09:32 |
| eudoxia | it's still a WIP, but yeah, I tried to dump every bit I knew about the subject before I forgot about it | 09:33 |
| kirka | I have found this Zyvex presentation today, It's quite optimistic: http://rghost.ru/40498627 | 09:34 |
| eudoxia | I remember it | 09:36 |
| kirka | It's a little diffirent from previous one | 09:36 |
| eudoxia | >We are working hard to be a leader in this field | 09:36 |
| eudoxia | well they better be | 09:36 |
| kirka | Heh | 09:36 |
| kirka | eudoxia Don't you know a working filesharing site where I could upload that book? | 09:37 |
| kirka | Piratebay bans me for some reason | 09:37 |
| eudoxia | I wouldn't know | 09:37 |
| kirka | Ok, I'll find something | 09:38 |
| eudoxia | last time I uploaded something illegal for the public was two years ago, and it was on megaupload ;_; | 09:38 |
| kirka | Heh | 09:38 |
| @kanzure | kirka: i'm sure you can find a russian release group that will take it | 09:39 |
| kirka | kanzure Yes, I think it's a good way to do it | 09:40 |
| kirka | I'll contact genesis guys | 09:40 |
| @kanzure | http://www.hoektronics.com/2012/09/21/makerbot-and-open-source-a-founder-perspective/ | 09:41 |
| kirka | BTW I find replicator 2 to be good product, regarding of OSS controversy | 09:41 |
| kirka | 0. mm precision is just what I need for robotics | 09:41 |
| kirka | *0.1 | 09:42 |
| kirka | *regardless | 09:42 |
| kirka | It takes a lot of time to build and set reprap to acheive such precision, isn't it? | 09:43 |
| @kanzure | no some of the prusa models have that precision | 09:44 |
| kirka | I have read builders' blogs, it requires months of calibration and rebuilding of reprap to achieve it | 09:45 |
| Mokbortolan_ | kirka: yeah, but how fast can it churn out my dynamically generated mini-turret parts? | 09:48 |
| kirka | Mokbortolan_ I don't know, heh | 09:49 |
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| kirka | I'm going to print this thing one way or another http://rghost.ru/40311893.view :) | 09:50 |
| eudoxia | It looks like something out of GITS | 09:52 |
| @kanzure | GITS is cliche these days | 09:52 |
| kirka | Thanks, that's a compliment to my design | 09:52 |
| kirka | Actually, I still need to remove 100 grams of plastic or integrate stronger servos | 09:52 |
| kirka | *200 | 09:52 |
| kirka | I will release everything to the public when it'll walk | 09:53 |
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| kirka | Interesting: http://www.youtube.com/watch?v=705raszSLGA | 10:29 |
| kirka | Merkle and Freitas @ 2012 | 10:29 |
| eudoxia | freitas needs to stand up and talk some more | 10:29 |
| jrayhawk | kanzure: I like the Frassetto study since she was feeding a bunch of sedentary nonobese people >5000 calories and they were still losing weight | 10:31 |
| Mokbortolan_ | stupid human metabolism, not doing what I expect | 10:35 |
| * Mokbortolan_ 's body is stubbornly carrying an emergency reserve of lipids. | 10:35 | |
| jrayhawk | Mokbortolan_: I was planning on watching a nutrition biochemistry seminar with some lesswrong nerds this weekend if you want to join in. | 10:36 |
| jrayhawk | I dropped fifty pounds and a pile of autoimmune and neurological problems. | 10:40 |
| jrayhawk | actually i guess they're legitimately singinst nerds | 10:41 |
| @kanzure | is it steve. | 10:42 |
| @kanzure | cause that doesn't count. | 10:42 |
| Mokbortolan_ | Sure! | 10:42 |
| jrayhawk | It sounds like he's getting re-employed | 10:42 |
| kirka | >Merkle: If a chemist looks at our structures, especially hydrocarbon ones he'll say "That's really boooring". | 10:46 |
| kirka | >You know, chemists are interested in structures that are falling apart from slightest touch | 10:46 |
| kirka | :) | 10:46 |
| jrayhawk | Mokbortolan_: Hmm. It's six hours broken up into 45-90 minue chunks. I guess Saturday is occupied for them, but today or Sunday might work. What's better for you? | 10:52 |
| Mokbortolan_ | Sunday, family eats up all my time on the weekday evenings :) | 10:53 |
| @kanzure | damn family | 10:53 |
| jrayhawk | Okay. I'd like to aim a start at 10:00AM, but that's up for being pushed back. If you show up to the lesswrong meetup on Saturday, we can discuss it all in one place. | 10:59 |
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| Mokbortolan_ | lesswrong meetup? | 11:07 |
| * Mokbortolan_ checks it out. | 11:07 | |
| Mokbortolan_ | I forget about meatspace sometimes... | 11:07 |
| jrayhawk | I've never been to one before, so I have no idea if they're well organized or worth going to. | 11:08 |
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| jrayhawk | Lucky Labs are crappy venues in general, so I am slightly suspicious. | 11:09 |
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| Mokbortolan_ | it's not that bad | 11:40 |
| Mokbortolan_ | a big room with tables | 11:40 |
| Mokbortolan_ | not *too* shabby | 11:40 |
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| * kirka sleeps | 12:48 | |
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| jrayhawk | The Lucky Labs typically have zero sound absorbtion, big crowds, and some of them have appallingly noisy industrial equipment. | 12:50 |
| docl | jrayhawk: That the portland meetup? I should really get up early and come one of these times. | 13:00 |
| docl | actually 10am isn't that bad. I just tend to get stuck online all morning. | 13:01 |
| jrayhawk | The LW thing is at noon on Saturday | 13:02 |
| jrayhawk | If you also want to show up for the biochemistry thing, that's cool, just let me know. | 13:11 |
| jrayhawk | Salem seems like a bit of a drive, though. | 13:11 |
| @kanzure | can someone check my work? this is a disassembled function and my java equivalent | 13:17 |
| @kanzure | http://diyhpl.us/~bryan/apk/readint.txt | 13:18 |
| @kanzure | does it make sense? i'm a little skeptical that i have it right | 13:18 |
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| delinquentme | kanzure, wish you were here man!! | 13:21 |
| delinquentme | nerd . down . PGH . 2012 =P | 13:21 |
| @kanzure | i would beat you into a bloody mess and force you to write better software | 13:27 |
| @kanzure | you wouldn't like me being around you | 13:27 |
| @kanzure | on a related note, i am now offering $800/hour software tutoring | 13:28 |
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| delinquentme | haha | 13:41 |
| delinquentme | wow | 13:41 |
| delinquentme | I'm looking for a portable hot spot | 13:41 |
| delinquentme | suggestions? | 13:41 |
| @kanzure | aircrack-ng | 13:41 |
| @kanzure | and giant wifi antennae | 13:42 |
| chris_99 | also most android phones let you setup a mobile hotspot | 13:42 |
| @kanzure | if you need a dummy gui then i suggest barnacle.apk | 13:43 |
| @kanzure | http://github.com/szym/barnacle | 13:43 |
| @kanzure | http://szym.net/apk/barnacle_latest.apk | 13:44 |
| delinquentme | except im gonna be on a train | 13:47 |
| delinquentme | lol | 13:47 |
| chris_99 | i've just 3G on a train before | 13:49 |
| @kanzure | i hate my isp | 14:00 |
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| @kanzure | i hate my isp https://github.com/kanzure/dpc2100-toolbelt | 14:20 |
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| bkero | I hate my new ISP :/ | 15:23 |
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| @kanzure | roksprok: sup? | 16:21 |
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| cpopell | Hmm | 16:28 |
| cpopell | This working? | 16:28 |
| Mokbortolan_ | yes | 16:40 |
| Mokbortolan_ | oh hey! | 16:40 |
| Mokbortolan_ | you're on my facebook | 16:40 |
| Mokbortolan_ | my house owner | 16:41 |
| Mokbortolan_ | whoopsd, wrong chat | 16:42 |
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| roksprok | kanzure:nm...I was planning on going to a cambrian genomics thing at biocurious last night, but its like 2 hours away and i'd have to leave half way through...i should get a car. I was super curious about them too | 16:58 |
| roksprok | oops no link...not sure how i did that... | 16:59 |
| @kanzure | roksprok: fenn lives with the cambrian genomics people | 17:02 |
| @kanzure | and i know half of them (not austen or reese, though) i think | 17:02 |
| roksprok | cool...so I guess they're legit then? | 17:02 |
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| @kanzure | roksprok: http://anselmlevskaya.com/ | 17:04 |
| roksprok | any papers on their process, or is it still 'stealth' | 17:05 |
| @kanzure | it's "stealth".. but my understanding is that they are just using sequencing to improve synthesis outcomes | 17:05 |
| roksprok | oooo...i thought that was like...par for the course? ha i guess i need to read some synthesis stuff | 17:08 |
| @kanzure | nmz787 has some mroe detailed notes about what they are doing.. there's possibly a microarray and laser involved somewhere. | 17:09 |
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| roksprok | cool i'll ask him for a linky, or are they on diyhpl.us? | 17:10 |
| @kanzure | nothing on the wiki sorry | 17:10 |
| @kanzure | they presented at the military thing with the fbi but DOD asked me to not take transcripts | 17:11 |
| @kanzure | as a result i have no clue what's going on | 17:11 |
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| roksprok | ah cool no probs | 17:19 |
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| @kanzure | oh look i spotted people talking about me on the internets | 18:35 |
| @kanzure | https://plus.google.com/u/0/113539880459449261884/posts/bQmiSNSNfpc | 18:35 |
| @kanzure | (thingiverse stuff) | 18:35 |
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| Juul | Hm, could it be that section 3.2 in their terms of use is simply left over from back when they had the "All rights reserved" option as secondary license? | 18:47 |
| Juul | I think that is likely | 18:47 |
| @kanzure | i'm just happy people are thinking about alternatives. if github comes out on top, i think it's a net win because then i don't have to write as many thing scrapers. | 18:48 |
| Juul | hah | 18:52 |
| @kanzure | i still think a package format needs to be decided on | 18:52 |
| @kanzure | a recent "openhardwarehub.com" fellow was trying to push an xml standard | 18:52 |
| @kanzure | and makerbot is trying to do a .thing format which is just zip of .stl and .obj (which is practically useless) | 18:53 |
| @kanzure | i don't think xml is going to win the race to the bottom on this one | 18:53 |
| @kanzure | but .stl isn't enough to define what a thing is | 18:53 |
| @kanzure | and what happens when the .thing has a .mmp file from nanoengineer? software explodes? | 18:54 |
| Juul | what is mmp? | 18:57 |
| @kanzure | nanoengineer has a custom file format for placing molecules around | 18:57 |
| @kanzure | sort of like pdb | 18:57 |
| @kanzure | (it also works with pdb) | 18:57 |
| Juul | ah | 18:57 |
| @kanzure | i think they just wanted something to experiment with, since messing with the .pdb format would attract anger :P | 18:58 |
| Juul | ok, that makes sense | 19:00 |
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| @kanzure | yashgaroth: yo | 19:11 |
| yashgaroth | whatup | 19:11 |
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| @kanzure | did dinosaurs sneeze? | 19:37 |
| yashgaroth | gonna go with yes | 19:38 |
| @kanzure | do birds? | 19:38 |
| @kanzure | i haven't noticed if birds do or not, i guess that would tell us about dinos | 19:38 |
| yashgaroth | reptiles apparently do | 19:39 |
| @kanzure | huh. | 19:39 |
| yashgaroth | humorous youtube videos confirm birds for sneeze | 19:39 |
| yashgaroth | brb | 19:39 |
| @kanzure | ah. | 19:39 |
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| @kanzure | nmz787: yo. | 19:44 |
| delinquentme | hands nmz787, beer | 19:44 |
| delinquentme | lol annnnddd im designing! | 19:45 |
| Juul | i have chickens | 19:45 |
| Juul | pretty sure i've seen the sneeze | 19:45 |
| brownies | great question kanzure . | 19:46 |
| brownies | did dinosaurs talk? since, you know, parrots can talk | 19:46 |
| Juul | yeah, but the only thing they said was "success! my time machine worked, now to AAAAAAAAAAAAA!" | 19:47 |
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| Juul | it became garbled after millions of years though | 19:48 |
| @kanzure | Juul: don't you live in downtown sf? how do you have chickens? | 19:48 |
| Juul | kanzure, i live in west oakland | 19:49 |
| @kanzure | oh | 19:49 |
| Juul | super cheap, and one bart stop away | 19:49 |
| delinquentme | Juul, you have chickens O_o;;? | 19:55 |
| delinquentme | as like pets or food? | 19:56 |
| Juul | delinquentme, for eggs! though they're not laying eggs yet. | 19:56 |
| delinquentme | OHHH SMART. | 19:56 |
| Juul | planning on feeding them somewhat from the overflowing dumpsters of berkeley bowl | 19:57 |
| Juul | someone even has a rooster in our neighborhood | 19:57 |
| Juul | not sure where | 19:57 |
| Juul | luckily the crowing doesn't wake me up | 19:57 |
| Juul | chickens are like $2 each | 19:58 |
| Juul | if you get them 1 day old | 19:58 |
| Juul | and they're shipped via normal usps | 19:58 |
| Juul | totally weird | 19:58 |
| Juul | you can only do it right after they hatch, since they then don't need food and water for two days | 19:58 |
| Juul | we didn't get ours like that though | 19:59 |
| Juul | usps isn't always gentle with their packages | 19:59 |
| delinquentme | haha | 20:05 |
| delinquentme | thats totally wild | 20:05 |
| delinquentme | how many do you have? and you can basically feed them human food? | 20:05 |
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| ParahSailin | why would someone have a rooster? | 20:19 |
| nmz787 | hi all | 20:21 |
| * nmz787 has no non-virtual beer | 20:22 | |
| nmz787 | i did however find that my 8 year old dexedrine seems to work | 20:22 |
| nmz787 | though the generic 7 year old stuff doesn't seem to | 20:22 |
| nmz787 | but that is a day time get-shit-done thing | 20:23 |
| nmz787 | man i've been lazy this summer | 20:23 |
| nmz787 | i worked twice this week, pretty hard, and was sore each day after | 20:23 |
| ParahSailin | drugs dont typically go bad | 20:24 |
| ParahSailin | they only go legally expired | 20:24 |
| nmz787 | first day i was splitting logs into firewood, and the second day i changed a basement window into stone like the foundation of the house | 20:24 |
| nmz787 | but i was doing it from the sidewalk, so i was constantly crouching and writhing around | 20:25 |
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| eudoxia | XML is so space inefficient | 20:25 |
| eudoxia | if only HTML had been implemented as S-expressions... | 20:26 |
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| @kanzure | handy dandy: http://jsonip.com/ | 20:37 |
| @kanzure | but i like http://httpbin.org/ | 20:37 |
| chevbird | ok | 20:41 |
| nmz787 | so my UC proxy access is ending on the 30th | 20:45 |
| nmz787 | which means less paper access :( | 20:45 |
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| @kanzure | nmz787: quick, signup for a cheap class and then fail it | 20:54 |
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| nmz787 | heh heh | 20:58 |
| nmz787 | well i'll still have RIT proxy access | 20:58 |
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| nmz787 | but the UC access was much better | 20:58 |
| skorket | evening all | 21:01 |
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| nmz787 | hi | 21:07 |
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| AdrianG | so um | 21:16 |
| AdrianG | anyone is taking metformin? | 21:16 |
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| gnusha | diyhpluswiki.git: a6315cf sydney biohacking group link | 21:29 |
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| nmz787 | so midget genetics, what's up with that | 22:03 |
| nmz787 | we have toy pets | 22:03 |
| nmz787 | 'toy' | 22:03 |
| @kanzure | is this your new comedy bit? it could use some work. | 22:05 |
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| nmz787 | no | 22:07 |
| nmz787 | i'm serious | 22:07 |
| nmz787 | it would be sweet to have a midget/'toy' elephant | 22:07 |
| nmz787 | i was also wondering if smaller dogs have less mental capacity than larger breeds | 22:07 |
| nmz787 | like, what's the difference genetically of a pinscher vs mini-pinscher | 22:08 |
| @kanzure | btw, most breeders aren't doing genetics work yet | 22:08 |
| nmz787 | if its common in multiple species, maybe we can translate it to others | 22:08 |
| nmz787 | like mini-cows | 22:08 |
| @kanzure | even if they think they have a winning pedigree, it's still very much a multi-generational waiting game | 22:09 |
| nmz787 | fresh milk, small, like juul's chickens | 22:09 |
| nmz787 | right but i'm talking big vs little | 22:09 |
| nmz787 | not neccesarily pure-bred, etc | 22:09 |
| nmz787 | go texas http://hardware.slashdot.org/story/12/08/23/1552222/wiki-weapon-project-wants-your-3d-printable-guns?sdsrc=popbyskid | 22:13 |
| @kanzure | does this include gene guns | 22:14 |
| nmz787 | I bet Cody would say 'Yes' | 22:16 |
| brownies | nmz787: http://www.youtube.com/watch?v=CjWYbcbpiWA | 22:25 |
| brownies | you can start by calling that giraffe breeder | 22:25 |
| @kanzure | it amuses me that there's someone who specializes in giraffe breeding | 22:26 |
| nmz787 | good cavitation demo | 22:43 |
| nmz787 | http://www.youtube.com/watch?v=77gWkl0ZUC8 | 22:43 |
| nmz787 | Shatter beer bottles: Bare-handed bottle smash | 22:43 |
| nmz787 | http://www.youtube.com/watch?v=14B8LynojI4 | 22:45 |
| nmz787 | DIY Liquid Nitrogen for Less Than $500 - Ben Krasnow | 22:45 |
| delinquentme | nmz787, is cavitation general pressure? | 22:46 |
| nmz787 | http://benkrasnow.blogspot.com/2008/08/diy-liquid-nitrogen-generator.html | 22:46 |
| nmz787 | its temporary vacuum | 22:46 |
| delinquentme | I thought cavitation is when some force is applied as to be sufficient to vaporize water and cause an expansion | 22:46 |
| nmz787 | but it can effect a powerful effect | 22:47 |
| delinquentme | IE what damages props on boats and the like | 22:47 |
| nmz787 | no | 22:47 |
| nmz787 | its vacuum | 22:47 |
| nmz787 | props push the water so powerfully that small vacuums form, and then the water slams back | 22:48 |
| nmz787 | like a mass spring system | 22:48 |
| nmz787 | it might have to do with using piston engines, but I'm not sure | 22:48 |
| nmz787 | it could just be prop design causing the weird pressure vortices | 22:48 |
| nmz787 | (a piston engine would emit vibrations) | 22:49 |
| nmz787 | aww, one of his parts is ebay sourced | 22:53 |
| @kanzure | nmz787: the biohacking group in sydney is trying to figure out a clever name | 23:06 |
| @kanzure | ozbio might work? | 23:06 |
| @kanzure | outback bio | 23:07 |
| @kanzure | sydney isn't really the outback | 23:07 |
| @kanzure | yashgaroth: you're good at this, you do it | 23:09 |
| yashgaroth | lemme ruminate on that shit | 23:10 |
| yashgaroth | throw another biohack on the barby | 23:10 |
| yashgaroth | a biohack ate my baby oooh that one | 23:10 |
| @kanzure | maybe i can convince them to adopt something gutsy like "australian bacteria enforcement agency" | 23:11 |
| yashgaroth | nmz787 I recall a case study of a man who had a brain tumor that took up 90% of his skull | 23:14 |
| nmz787 | lol | 23:14 |
| nmz787 | wow | 23:14 |
| yashgaroth | he was kind of dumb but married with kids so | 23:14 |
| nmz787 | was he a midget? | 23:15 |
| @kanzure | i was trying to find that study once, actually | 23:15 |
| nmz787 | yashgaroth: can you get this | 23:15 |
| nmz787 | http://www.ingentaconnect.com/content/iafp/jfp/1999/00000062/00000002/art00016?token=004d12f99a41333c4a2f7a736a5e3b20674723773b254f6d6222346b62687630502163b172b11 | 23:15 |
| @kanzure | i recalled it was something like "10% brain size" or something | 23:15 |
| yashgaroth | how would I get an article | 23:15 |
| nmz787 | proxy access somewhere? | 23:15 |
| @kanzure | i don't think he realizes that your source is me | 23:15 |
| yashgaroth | ^ | 23:15 |
| nmz787 | well, shit | 23:16 |
| @kanzure | and none of mine are green on that article | 23:16 |
| nmz787 | do i have to buy this damn article | 23:16 |
| yashgaroth | I haven't been able to get articles for a couple years now | 23:16 |
| nmz787 | hmm | 23:16 |
| @kanzure | nmz787: http://reddit.com/r/scholar | 23:16 |
| yashgaroth | ingenta's a bunch of dicks about access, moreso than most | 23:16 |
| @kanzure | or you can go to http://i-nobel.com/bbs/ and plead to the chinese | 23:16 |
| nmz787 | I wonder if there's a list of cheapest schools vs access to # of journals | 23:16 |
| @kanzure | community colleges would be your best bet | 23:16 |
| @kanzure | especially in rich cities | 23:16 |
| @kanzure | well wait, no | 23:17 |
| yashgaroth | I imagine their access is still pretty crap unless they're part of a state network | 23:17 |
| @kanzure | they don't have much reason to subscribe to research journals | 23:17 |
| yashgaroth | that too | 23:17 |
| @kanzure | austincc.edu had some access, but it wasn't enough for me to stick around | 23:17 |
| nmz787 | man i need to have a kid quick and teach it to read and write chinese for me, so I can get this article | 23:18 |
| nmz787 | paywalls are getting steeper all the time... | 23:18 |
| @kanzure | ask on diybio | 23:18 |
| nmz787 | now that is part of my comedy routine :P | 23:18 |
| @kanzure | a lot of college students are on diybio, they have access | 23:19 |
| nmz787 | I think the selection is just X-gal on YPD agar plates | 23:24 |
| nmz787 | because the strain has constitutive lactase | 23:24 |
| nmz787 | so without IPTG or lactose to induce the non target strain's lac operon, you can differentiate | 23:25 |
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| nmz787 | well I got this one at least http://www.nrcresearchpress.com/doi/abs/10.1139/w00-107 | 23:33 |
| nmz787 | yashgaroth: have you ever seen anyone in your field using either K.lactis or K.marxianus? | 23:34 |
| yashgaroth | nope | 23:34 |
| yashgaroth | are they interesting | 23:34 |
| nmz787 | hmm, I guess its limited to industrial scale fermenters | 23:34 |
| nmz787 | yeah | 23:34 |
| nmz787 | most efficient eukaryotes for protein production | 23:35 |
| nmz787 | http://www.amb-express.com/content/2/1/38 | 23:35 |
| nmz787 | http://oba.od.nih.gov/oba/RAC/meetings/Dec2010/K_lactis_NEB_09172010.pdf | 23:35 |
| nmz787 | used to make rennet and laundry enzymes | 23:35 |
| yashgaroth | yeast is still kinda niche, 90% is either e.coli or CHO | 23:36 |
| yashgaroth | oh yeah industrial enzymes are a whole different story | 23:36 |
| nmz787 | i figure since its GRAS | 23:36 |
| nmz787 | its what i'll use for any projects | 23:36 |
| yashgaroth | what cell lines aren't GRAS these days | 23:37 |
| nmz787 | oh? | 23:37 |
| nmz787 | I wouldn't drink e.coli | 23:37 |
| nmz787 | or CHO | 23:37 |
| yashgaroth | I would for some money | 23:37 |
| nmz787 | i would drink milk fermented with k.lactis | 23:37 |
| nmz787 | (since its already there naturally) | 23:37 |
| yashgaroth | "naturally" | 23:38 |
| nmz787 | heh | 23:38 |
| nmz787 | well, kefir has been consumed for a long time | 23:38 |
| nmz787 | that's safe enough for me | 23:38 |
| yashgaroth | you're full of e.coli naturally, and cho is no different from eating some good ol' hamster ovaries | 23:38 |
| nmz787 | but milk is cheap and everywhere | 23:38 |
| nmz787 | i doubt i would really have to mix in anything | 23:39 |
| nmz787 | unless I wanted to supplement some engineered pathway | 23:39 |
| yashgaroth | what's wrong with using pichia | 23:41 |
| nmz787 | doesnt that eat methanol? | 23:42 |
| yashgaroth | or s cerevisiae | 23:42 |
| yashgaroth | yeah dude bonus you don't get methanol poisoning cuz the yeast cleared that shit out for you | 23:43 |
| nmz787 | hmm | 23:43 |
| yashgaroth | and it's still far more characterized than some lactase yeast | 23:43 |
| nmz787 | well is there no reason then for the industrial use of k.lactis over the others? | 23:43 |
| yashgaroth | where do they claim 'most efficient eukaryotes'? | 23:43 |
| nmz787 | hmm | 23:45 |
| nmz787 | i dont think i dreamt that | 23:45 |
| yashgaroth | I see 'most theoretically efficient at producing alcohol' which is nice but it's not protein | 23:45 |
| nmz787 | http://www.ncbi.nlm.nih.gov/pubmed/19087200 | 23:50 |
| nmz787 | 52 min cell cycle | 23:50 |
| yashgaroth | that's only a big concern if you need to get a 1000L bioreactor running Right Now, but otherwise I'm worried it'd be spending too much time replicating and not enough time producing my proteins | 23:52 |
| nmz787 | hmm | 23:52 |
| nmz787 | it's nice that it's already widely eaten | 23:53 |
| nmz787 | makes it seem safe to me | 23:53 |
| yashgaroth | I wouldn't drink a jug of it | 23:53 |
| nmz787 | the crabtree-negative effect seems like it would be a good thing | 23:53 |
| nmz787 | which means when there's a lot of glucose, it doesn't make ethanol (but still can under anoxic conditions) | 23:54 |
| nmz787 | wouldn't that mean the energy in is going to stuff like protein production? | 23:54 |
| nmz787 | instead of ethanol (byproduct) | 23:54 |
| nmz787 | and ethanol enzymes | 23:55 |
| yashgaroth | I wouldn't imagine any cell would ferment glucose to ethanol if it had oxygen | 23:55 |
| yashgaroth | and was aerobic | 23:55 |
| yashgaroth | I guess it could save some energy by not bothering with that pathway, but if it's already low expression you're not saving much | 23:56 |
| nmz787 | says s cerevisae is crabtree-positive | 23:56 |
| nmz787 | http://mic.sgmjournals.org/content/114/2/267.full.pdf | 23:56 |
| nmz787 | wait | 23:57 |
| nmz787 | you said you would drink a gallon of it | 23:58 |
| nmz787 | but doesnt drinking a gallon of milk make you puke as it is, unfermented? | 23:58 |
| yashgaroth | when did I say that | 23:58 |
| yashgaroth | [23:53:53] .:yashgaroth:. I wouldn't drink a jug of it | 23:58 |
| nmz787 | http://en.wikipedia.org/wiki/Milk_chugging#Medical_explanation | 23:59 |
| nmz787 | oh | 23:59 |
| yashgaroth | I'm more worried about it getting to my intestines and doing all sorts of wacky shit | 23:59 |
| --- Log closed Sat Sep 22 00:00:14 2012 | ||
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