| --- Log opened Mon Oct 01 00:00:42 2012 | ||
| -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 244 seconds] | 00:28 | |
| -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap | 00:39 | |
| -!- sivoais [~zaki@199.19.225.239] has quit [Ping timeout: 260 seconds] | 00:46 | |
| -!- sivoais [~zaki@199.19.225.239] has joined ##hplusroadmap | 00:59 | |
| -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Read error: Connection reset by peer] | 01:14 | |
| -!- marainein [~net@2001:388:608c:6cb5:6553:5ac9:9ce9:79b6] has joined ##hplusroadmap | 01:19 | |
| -!- Mariu [Jimmy98@89.41.57.33] has quit [Ping timeout: 265 seconds] | 04:09 | |
| -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap | 04:19 | |
| -!- Mariu [Jimmy98@89.41.57.33] has quit [Client Quit] | 04:21 | |
| -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap | 04:26 | |
| -!- Mokstar [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap | 05:27 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 05:28 | |
| -!- Mokstar1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 255 seconds] | 05:29 | |
| -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap | 05:36 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 246 seconds] | 05:47 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 05:54 | |
| -!- Mokstar1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap | 05:57 | |
| -!- Mokstar [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 260 seconds] | 05:57 | |
| -!- Mokstar [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap | 06:10 | |
| -!- delinquentme [~asdfasdf@rrcs-74-219-159-104.central.biz.rr.com] has quit [Quit: Leaving] | 06:11 | |
| -!- Mokstar1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 252 seconds] | 06:11 | |
| skorket | I'm not affiliated with Cornell | 06:13 |
|---|---|---|
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 260 seconds] | 06:19 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 06:35 | |
| -!- Juul [~Juul@208.87.217.74] has joined ##hplusroadmap | 07:04 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 246 seconds] | 07:11 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 07:13 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 248 seconds] | 07:21 | |
| -!- Jaakko96 [~Jaakko@94-194-89-130.zone8.bethere.co.uk] has joined ##hplusroadmap | 07:21 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 07:22 | |
| -!- Mokstar1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap | 07:32 | |
| -!- Mokstar [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 245 seconds] | 07:33 | |
| -!- Jaakko96 [~Jaakko@94-194-89-130.zone8.bethere.co.uk] has quit [Quit: Nettalk6 - www.ntalk.de] | 07:50 | |
| @kanzure | this it kakarot | 07:51 |
| @kanzure | is it | 07:51 |
| @kanzure | saiyan failure | 07:51 |
| -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection] | 08:00 | |
| -!- sivoais [~zaki@199.19.225.239] has quit [Ping timeout: 245 seconds] | 08:00 | |
| -!- SDr [~SDr@unaffiliated/sdr] has joined ##hplusroadmap | 08:04 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 246 seconds] | 08:07 | |
| -!- sivoais [~zaki@199.19.225.239] has joined ##hplusroadmap | 08:09 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 08:13 | |
| -!- Mariu [Jimmy98@89.41.57.33] has quit [Ping timeout: 265 seconds] | 08:28 | |
| -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap | 08:33 | |
| -!- Mokstar1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Quit: Leaving.] | 08:41 | |
| -!- Mokstar [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap | 08:41 | |
| @kanzure | https://docs.google.com/spreadsheet/ccc?key=0AiaWH3PL_9CndG1GbmtSZ1I3aGdkQkE4YVhwZ3o5c1E#gid=0 | 08:44 |
| @kanzure | openpcr bom | 08:44 |
| @kanzure | why isn't there a way to visually diagnose whether or not a chute will deploy? | 08:51 |
| -!- augur [~augur@129-2-129-33.wireless.umd.edu] has joined ##hplusroadmap | 09:00 | |
| jrayhawk | explosives are kinda finnicky | 09:00 |
| jrayhawk | this is especially true for hobbiests who tend to let volatile self-oxidating compounds sit around for a while | 09:01 |
| @kanzure | well, for starters, i always see parachutes are in dark packages that you can't see into | 09:01 |
| chris_99 | what kind of parachute do you mean, one that you jump out a plane in? | 09:02 |
| @kanzure | yes | 09:02 |
| jrayhawk | oh, nevermind | 09:02 |
| @kanzure | haha yeah i was wondering about that.. "what? are we packing parachutes with explosives now? neat." | 09:02 |
| chris_99 | hehe | 09:03 |
| jrayhawk | that's how it's done for most applications | 09:03 |
| chris_99 | im not sure even if it was packed in a transparent bag, if you'd be able to tell it was packed right? | 09:03 |
| @kanzure | i'm not sure packing errors are the only source of problems | 09:03 |
| @kanzure | you could possibly use markers on the material to tell if it was packed/folded correctly | 09:04 |
| @kanzure | but realistically if you actually need to use a parachute in an emergency, you don't have time to re-pack anyway. but you might have time to get another one. | 09:04 |
| chris_99 | yeah that sounds like a sensible idea | 09:04 |
| @kanzure | and, if these chutes are failing because of manufacturing error, that sounds important too | 09:05 |
| -!- augur [~augur@129-2-129-33.wireless.umd.edu] has quit [Read error: Connection reset by peer] | 09:06 | |
| @kanzure | what's the failure rate anyway? 1 in 10,000? | 09:06 |
| -!- augur [~augur@129-2-129-33.wireless.umd.edu] has joined ##hplusroadmap | 09:06 | |
| @kanzure | "Exact numbers are difficult to estimate, but approximately one in a thousand sports main parachute openings malfunction, and must be cut away," | 09:09 |
| @kanzure | 1/1000? wtf? that's a terrible failure rate. | 09:09 |
| @kanzure | "In the U.S., the average fatality rate is considered to be about 1 in 80,000 jumps" | 09:10 |
| @kanzure | whaat. | 09:10 |
| chris_99 | very odd, i guess that means the team mates must save you a lot? | 09:11 |
| @kanzure | well, i guess this isn't the same as a "bad" chute being packed by a manufacturer - maybe those error rates are a little better, hopefully by a few orders of magnitude. | 09:13 |
| @kanzure | "German parachutes of 1918 exhibited a failure rate around 50% when used under combat conditions." wtf? | 09:16 |
| chris_99 | does that failure rate include getting shot down | 09:18 |
| Mariu | lol | 09:19 |
| @kanzure | "the reliability of the system can be assumed constrained by the reliability of the reserve chute" what? no.. | 09:23 |
| @kanzure | http://www.dtic.mil/dtic/tr/fulltext/u2/787299.pdf | 09:23 |
| @kanzure | "With 2,040,000 recorded jumps and no reported failures, the reliability must, therefore, be assumed one." | 09:24 |
| -!- EnLilaSko- [~Nattzor@m176-68-151-47.cust.tele2.se] has joined ##hplusroadmap | 10:14 | |
| -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has quit [Ping timeout: 245 seconds] | 10:14 | |
| -!- augur [~augur@129-2-129-33.wireless.umd.edu] has quit [Remote host closed the connection] | 10:16 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] | 10:23 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 10:24 | |
| -!- sivoais [~zaki@199.19.225.239] has quit [Ping timeout: 255 seconds] | 11:03 | |
| -!- sivoais [~zaki@199.19.225.239] has joined ##hplusroadmap | 11:07 | |
| -!- wizzbrah [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has quit [Ping timeout: 245 seconds] | 11:34 | |
| @kanzure | "Delta Airlines has purchased their own oil refinery to get out from under the thumb of speculators like GS and have more control over their own fuel prices." | 11:43 |
| @kanzure | http://finance.fortune.cnn.com/2012/04/12/delta-oil-refinery/ | 11:43 |
| @kanzure | http://slashdot.org/~SteveWoz | 11:47 |
| @kanzure | "I was a singlularity denier, for one thing. But I have to reverse myself and admit that I'm wrong. Oddly, it was Ray, presenting to an audience in Vienna, which convinced me otherwise." | 11:52 |
| @kanzure | chemical supplier search app thing http://mobilereagents.com/ | 12:01 |
| @kanzure | http://mobilereagents.com/getStats.html | 12:01 |
| @kanzure | http://amazon-more64.eidogen-sertanty.com/MORESearchWithGet.php?stype=sss&sfield=smi&sinfo=CC1=C(C(C)=NO1)C1=CC=CC=C1 | 12:01 |
| @kanzure | damn their interface sucks | 12:01 |
| @kanzure | http://amazon-more64.eidogen-sertanty.com/molDescripsView.php?cd_id=808978 | 12:01 |
| @kanzure | why do they have a QR code on a web page ? | 12:01 |
| @kanzure | damn. no one-click ordering either. | 12:02 |
| @kanzure | how am i supposed to get my 5-methoxy-2-[4-phenyl-3-(trifluoromethyl)-1,2-oxazol-5-yl]phenol | 12:02 |
| -!- ThomasEgi [~thomas@p54BAD8A5.dip.t-dialin.net] has joined ##hplusroadmap | 12:38 | |
| -!- ThomasEgi [~thomas@p54BAD8A5.dip.t-dialin.net] has quit [Changing host] | 12:38 | |
| -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap | 12:38 | |
| -!- augur [~augur@129-2-129-33.wireless.umd.edu] has joined ##hplusroadmap | 13:24 | |
| -!- augur [~augur@129-2-129-33.wireless.umd.edu] has quit [Remote host closed the connection] | 13:49 | |
| -!- wazzbri [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has joined ##hplusroadmap | 14:07 | |
| -!- technicus [~technicus@198.150.12.29] has joined ##hplusroadmap | 14:16 | |
| @kanzure | haha | 14:17 |
| @kanzure | "DIY Gun Project Put On Hold As Creators Have Their 3D Printer Seized" | 14:17 |
| @kanzure | http://www.webpronews.com/diy-gun-project-put-on-hold-as-creators-have-their-3d-printer-seized-2012-10 | 14:17 |
| @kanzure | oh, nevermind. just linkbait. i am so disappointed. | 14:18 |
| @kanzure | "Wired reports that Stratasys apparently didn’t like what the guys at Defense Distributed were doing with their printer. Once they caught wind of the Wiki Weapon project, they immediately canceled the lease and reclaimed the printer." | 14:18 |
| @kanzure | yeah, well, that's what you get for using stratasys | 14:18 |
| -!- wazzbri [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has quit [Ping timeout: 245 seconds] | 14:25 | |
| -!- technicus [~technicus@198.150.12.29] has quit [Ping timeout: 246 seconds] | 14:30 | |
| -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap | 14:31 | |
| -!- chevbird [~chevbird@wrls-249-198-160.wrls-client.fas.harvard.edu] has joined ##hplusroadmap | 14:34 | |
| -!- chevbird [~chevbird@wrls-249-198-160.wrls-client.fas.harvard.edu] has quit [Client Quit] | 14:34 | |
| -!- alusion [~alusion@pool-173-79-17-100.washdc.fios.verizon.net] has quit [Read error: Operation timed out] | 14:40 | |
| -!- alusion [~alusion@pool-173-79-17-100.washdc.fios.verizon.net] has joined ##hplusroadmap | 14:40 | |
| -!- wazzbri [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has joined ##hplusroadmap | 15:01 | |
| chris_99 | heh, that was such a pointless idea i thought | 15:06 |
| chris_99 | making guns with 3d printers | 15:06 |
| jrayhawk | huh, that's a good idea. i could use a vertical foregrip. | 15:34 |
| -!- marainein [~net@2001:388:608c:6cb5:6553:5ac9:9ce9:79b6] has quit [Quit: Ex-Chat] | 15:35 | |
| jrayhawk | my guns are, by volume, largely plastic | 15:35 |
| chris_99 | what kind of guns? | 15:35 |
| jrayhawk | http://www.fivesevenammo.com/shop/media/FS2000TacticalBlack copy.jpg | 15:36 |
| jrayhawk | http://en.wikipedia.org/wiki/File:Steyr_M-A1_M40-A1_mit_1_Magazin_und_Blockierschl%C3%BCssel.jpg | 15:37 |
| chris_99 | i don't know anything about guns mind, that looks really weird looking heh | 15:37 |
| jrayhawk | It's a fairly exotic architecture. | 15:37 |
| chris_99 | what kind of gun is it, automatic? | 15:38 |
| jrayhawk | It's a semi-auto. Bullpup with front brass ejection, which I believe only one other company makes. | 15:38 |
| jrayhawk | specifically, the Kel-Tec RFB, which is the .308 competition to the FS2000's .223 | 15:39 |
| jrayhawk | http://en.wikipedia.org/wiki/File:Walther_P22_Corrected.jpg oh yeah, i guess this is mostly plastic, too | 15:41 |
| jrayhawk | i apparently really like plastic-frame guns | 15:41 |
| jrayhawk | http://gunblast.com/images/KelTec-RFB/DSC09886.JPG the bullpup is a pretty cool design for efficiency; these two guns have the same barrel length | 15:43 |
| jrayhawk | http://www.gunblast.com/images/FS2000/DSC04724.jpg same here | 15:44 |
| chris_99 | isn't plastic er dangerous though | 15:45 |
| chris_99 | in that it could just melt | 15:46 |
| jrayhawk | Would depend on the plastic. | 15:47 |
| jrayhawk | ABS, which I think is what most people use for extrusions, I wouldn't trust anywhere near the barrel, but elsewhere would do okay. | 15:47 |
| jrayhawk | 3D printer-style extrusions | 15:48 |
| @kanzure | according to bre pettis, thingiverse is built on wordpress | 15:48 |
| @kanzure | bre pettis is also an idiot so who knows | 15:52 |
| @kanzure | jrayhawk: can you go stalk down justin dormandy in portland and see what he's up to? | 15:53 |
| jrayhawk | that sounds like effort | 15:54 |
| @kanzure | ok well if you run into him, tell him that the internet is looking for him | 15:54 |
| jrayhawk | according to glock, fiberglass-reinforced nylon66 is good, which, according to dupont, has a melting point around 500F | 15:56 |
| jrayhawk | i think glocks usually have floating barrels constrained by metal guides, so i guess the plastic bits avoids direct thermal coupling | 15:58 |
| chris_99 | i think what that 3d printer person was trying to make was make single use guns | 16:01 |
| @kanzure | "you only need one shot" hah | 16:04 |
| @kanzure | "you only live long enuogh to make one shot" | 16:04 |
| chris_99 | hehe | 16:05 |
| jrayhawk | actually adrenaline can keep you operating fairly normally even with a lot of bullet holes in you for several minutes | 16:05 |
| jrayhawk | one shot is thus only enough if you aren't in a hurry | 16:06 |
| jrayhawk | or if you manage to hit a major nervous or vascular pathway | 16:07 |
| -!- technicus [~technicus@ip-64-134-161-42.public.wayport.net] has joined ##hplusroadmap | 16:16 | |
| jrayhawk | huh, yeah, 'no moving parts' suggests single fire | 16:18 |
| jrayhawk | i am not real clear on how they intend to electrically trigger a rimfire .22 | 16:20 |
| chris_99 | could they use a mini explosive | 16:24 |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] | 16:33 | |
| jrayhawk | then they have lost the reason for using standard ammunition | 16:38 |
| -!- technicus [~technicus@ip-64-134-161-42.public.wayport.net] has quit [] | 16:47 | |
| -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] | 16:56 | |
| @kanzure | Juul: have you implemented key signing of biobricks in biofab yet? | 17:06 |
| -!- magicsmoke [~magicsmok@c-98-198-91-31.hsd1.tx.comcast.net] has joined ##hplusroadmap | 17:13 | |
| -!- magicsmoke [~magicsmok@c-98-198-91-31.hsd1.tx.comcast.net] has quit [Client Quit] | 17:13 | |
| ParahSailin_ | just gonna throw it out there-- "gun" is not one of the things one should be trying to 3d print in plastic | 17:14 |
| Mariu | :p | 17:20 |
| Mariu | water guns | 17:21 |
| -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap | 17:21 | |
| -!- irctc887 [443dff8d@gateway/web/freenode/ip.68.61.255.141] has joined ##hplusroadmap | 17:25 | |
| -!- irctc887 [443dff8d@gateway/web/freenode/ip.68.61.255.141] has quit [Ping timeout: 245 seconds] | 17:29 | |
| yashgaroth | jrayhawk you have an fs2000? that's some classy shit | 17:30 |
| -!- EnLilaSko- [~Nattzor@m176-68-151-47.cust.tele2.se] has quit [Quit: - nbs-irc 2.39 - www.nbs-irc.net -] | 17:31 | |
| -!- Juul [~Juul@208.87.217.74] has quit [Ping timeout: 246 seconds] | 17:38 | |
| -!- eudoxia [~eudoxia@r186-54-253-153.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 17:57 | |
| eudoxia | hello friends | 17:58 |
| -!- Lucas__ [6c115747@gateway/web/freenode/ip.108.17.87.71] has joined ##hplusroadmap | 18:02 | |
| -!- eudoxia [~eudoxia@r186-54-253-153.dialup.adsl.anteldata.net.uy] has quit [Quit: Lost terminal] | 18:08 | |
| -!- Bioguy [~justin@c-71-237-180-48.hsd1.or.comcast.net] has joined ##hplusroadmap | 18:22 | |
| @kanzure | Bioguy: hi | 18:23 |
| Bioguy | Hey! | 18:23 |
| Bioguy | So... have we met before? | 18:23 |
| @kanzure | Bioguy: do you go to PSAS? | 18:23 |
| @kanzure | no | 18:23 |
| @kanzure | we have a few portland people in here, i just thought you should probably know them | 18:24 |
| @kanzure | like Mokstar or jrayhawk or lichen | 18:24 |
| @kanzure | and soon nmz787 will be moving in.. he'll probably want to setup some biohacking thing somewhere. | 18:25 |
| Bioguy | Bryan Bishop...that sounds familiar - did you post on the google DIYbio group? | 18:25 |
| @kanzure | according to google, i am the person who sends the most email to diybio, yes :\ | 18:26 |
| -!- Bioguy is now known as BioGuy | 18:28 | |
| BioGuy | So...ok you figured I was in Portland from my ip address, my /whois has my first name...how did you figure my last name and gmail? | 18:29 |
| * BioGuy scrathing head | 18:30 | |
| @kanzure | you're the only google result for justin, diybio, hackerspace and portland | 18:30 |
| @kanzure | plus, you show up in my email inbox | 18:31 |
| BioGuy | Oh! Did my post show up on the google groups? | 18:31 |
| @kanzure | yes | 18:31 |
| BioGuy | Oh... where is it? I replied to 'Current examples of DIYbio that I see...' but it didn't look my post actually went through thought I was having browser issues. | 18:34 |
| @kanzure | browser issues? | 18:35 |
| @kanzure | it's email | 18:35 |
| BioGuy | I think its both...appears as a forum if you go to groups.google.com | 18:36 |
| BioGuy | ...anywho - whats with the channel name? | 18:37 |
| yashgaroth | this isn't solely a diybio channel | 18:38 |
| @kanzure | originates from a technology roadmap for open source hardware for genetic engineering, brain implants, things like that | 18:38 |
| brownies | ##diybiopunkhackingninjas was taken | 18:38 |
| BioGuy | OH! I take it the 'h' is for human? | 18:38 |
| @kanzure | more or less yeah | 18:39 |
| BioGuy | brownies: nice! | 18:39 |
| BioGuy | So what is PSAS? | 18:39 |
| @kanzure | portland state aerospace society. they do lots of open source rocket things. | 18:40 |
| BioGuy | So are you in Portland? | 18:41 |
| @kanzure | no. i listed some people in here who are, though. see above.. | 18:42 |
| -!- cpopell [ad42e490@gateway/web/freenode/ip.173.66.228.144] has joined ##hplusroadmap | 18:42 | |
| BioGuy | I should try an meet them. You know when they're usually around? | 18:45 |
| @kanzure | Mokstar: do things! | 18:45 |
| @kanzure | jrayhawk: your presence has been summoned | 18:45 |
| BioGuy | So just to double check the message you got on the google mailing list was my rant about reagents not being accessible right? | 18:46 |
| @kanzure | yes | 18:47 |
| @kanzure | if you have any project plans to do cheap protein production, run them by us | 18:47 |
| @kanzure | and we can dump cash | 18:47 |
| jrayhawk | ugh here you are wanting me to actually do thing again | 18:53 |
| jrayhawk | s/thing/things | 18:53 |
| -!- marainein [~net@2001:388:608c:6cb5:447c:c75e:9c2e:77b2] has joined ##hplusroadmap | 18:54 | |
| jrayhawk | Hi Justin; I like better living through biochemistry, but I am not much of one for diybio type stuff. | 18:55 |
| jrayhawk | I can take you out bouldering sometime, though. | 18:55 |
| BioGuy | Hey you ever go to the bouldering gym on Macadam | 18:57 |
| jrayhawk | Yeah. | 18:58 |
| BioGuy | Ya! I want to go back there! | 18:59 |
| jrayhawk | I'm available for that tomorrow or Sunday; I'd prefer to start 16:00 or earlier and can probably come kidnap you from wherever. | 19:00 |
| BioGuy | So all the people here in pdx, what are yours and their background | 19:01 |
| BioGuy | BAH! let me rephrase that, jrayhawk, whats your background and the other guys? | 19:02 |
| BioGuy | Kanure: thanks for rescuing me from that very empty room :-) | 19:02 |
| -!- delinquentme [~asdfasdf@rrcs-74-219-159-104.central.biz.rr.com] has joined ##hplusroadmap | 19:02 | |
| jrayhawk | Professionally, I do Linux system/network administration and internet hosting and Mokstar does Windows system administration. I'm also roped into talking to and helping out rationalist/futurist communities for some reason. | 19:05 |
| BioGuy | rationalist/futurist communities? | 19:06 |
| jrayhawk | ##hplusroadmap, in particular, is about practical transhumanism | 19:09 |
| BioGuy | Not really too familiar with the term | 19:11 |
| BioGuy | I'm assuming technologically advancing beyond our own biology essentially? | 19:11 |
| jrayhawk | or biologically advancing | 19:15 |
| BioGuy | so how is your biochemistry knowledge? | 19:16 |
| jrayhawk | I have a minimal practical set for manipulating my endocrinology | 19:17 |
| @kanzure | jrayhawk is not representative :P | 19:17 |
| @kanzure | when nmz787 roles into town, portland will gain a molecular biology guy | 19:18 |
| BioGuy | manipulating your endocrinology? Can you elaborate? | 19:18 |
| jrayhawk | mok and bkero have interesting hardware projects from time to time | 19:19 |
| @kanzure | i think he means that he likes to eat | 19:19 |
| BioGuy | I just completed an associates degree in bisocience technology. Mostly it focused on bacterial transformations, genetic engineering, cell and tissue culture, some basic molecular bio, immunochemistry (western blots, elisa assays, etc), protein analysis and purification | 19:20 |
| @kanzure | oh good | 19:20 |
| @kanzure | what are you doing now? | 19:21 |
| * bkero hsa hardware | 19:22 | |
| bkero | What're you interested in? | 19:22 |
| jrayhawk | it is true, i do like to eat | 19:23 |
| BioGuy | Not sure exactly where you guys are relative to me so if you have any questions definitely feel free to ask. But, I was curious why TAE and TBE is always used as the running buffer for DNA gel electrophoresis. It turns out there isn't any good reason. So I'm working on comparing TAE/TBE to TA/TB and sodium boric acid/lithium boric acid. I think with the lithium boric acid I might be able to do some | 19:23 |
| BioGuy | very small scale sequencing on an agarose gel instead of having to use polyacrylamide. | 19:23 |
| @kanzure | yashgaroth: ping | 19:24 |
| yashgaroth | uhh I'd imagine you add the E to block nucleases | 19:24 |
| yashgaroth | I suppose it doesn't matter if you run it immediately and don't need to do anything but visualize the bands, but EDTA isn't expensive | 19:25 |
| BioGuy | It makes sense to have the EDTA in the loading buffer, but not the running buffer. It ends up just adding an extra ion that doesn't need to be there and makes the gel run more prone to heading up and generating increasing amounts of current at a constant voltage. | 19:26 |
| BioGuy | Maybe not expensive...but unnecessary. from some of the research I've done it looks like there's no qualitative difference between TAE/TBE/TA/TB - but I want to see what the current, ph, and heat changes are during a run | 19:28 |
| yashgaroth | if you need a super-fast gel it might be worthwhile | 19:28 |
| BioGuy | The nice thing about lithium boric acid and sodium boric acid it has a very minimal heat feedback loop compared to TAE/TBE - so you wont melt your gels | 19:29 |
| BioGuy | What I really want to see though is if I can get 1 base pair resolution on a simple agarose gel, which from some of the reading I've done it looks like you can without melting the gel. | 19:30 |
| yashgaroth | but if I can run my gels faster, I won't be able to dick around on the internet while claiming that I'm working | 19:30 |
| yashgaroth | sure you can if you use a super high concentration of agarose | 19:31 |
| BioGuy | LOL!! | 19:31 |
| -!- Lucas__ [6c115747@gateway/web/freenode/ip.108.17.87.71] has quit [Ping timeout: 245 seconds] | 19:31 | |
| yashgaroth | I must admit I haven't thought about the subject much since I have a 50x TAE stock in the lab and don't have to pay for it | 19:32 |
| BioGuy | Thats interesting...I should add that to the list and see what happens if you just increase the agarose concentration and stuck with TBE/TAE | 19:32 |
| yashgaroth | I'm sure you can throw in some wacky chemical to increase the resolution | 19:33 |
| BioGuy | I'm thinking more along the lines of IF this could work people might have a cheap way of testing for specific SNPs on their own. | 19:33 |
| yashgaroth | ah wait they use acrylamide for sequencing gels, nm | 19:34 |
| BioGuy | exactly! | 19:34 |
| @kanzure | why not aptamers or antibody reporters for checking snps? | 19:34 |
| cpopell | sup folks | 19:35 |
| BioGuy | But imagine if you could do it simply with agarose - it would be cheaper and you wouldn't have to concern yourself at all with the carcinogenicity of polyacrylamide | 19:35 |
| yashgaroth | hey, only monoacrylamide is toxic...but yeah | 19:35 |
| BioGuy | Lol ok fine, its been a while...what is it? Bis-acrylamide? I forget which is the toxic one | 19:36 |
| yashgaroth | idk man, all I know is don't make the gels yourself if you're like me and enjoy pouring aerosolizable powders rather than scooping | 19:37 |
| BioGuy | kanure - never heard of aptamers. By antibody reporters do yo mean something like an antibody with a fluorophore or HRP conjugated? | 19:37 |
| @kanzure | aptamers are just loops of dna that very strongly hybridize to things - but they take a shitload of iterations to get the aptamers just right | 19:38 |
| @kanzure | and yes i mean antibodies with a flurophore (or something) | 19:38 |
| BioGuy | yashgaroth: whats your background? | 19:38 |
| yashgaroth | antibodies suck at binding to DNA | 19:38 |
| @kanzure | aptamers are pretty good at it though | 19:38 |
| yashgaroth | I've a BS in molbio, been working in industry for 3 years now | 19:38 |
| @kanzure | eh whatever, gels are worth it anyway | 19:38 |
| cpopell | t-minus 14 days until I hear back from DARPA :D | 19:39 |
| yashgaroth | I guess you could try some variant of a southern blot | 19:39 |
| BioGuy | I'll have to check out more about aptamers... when you say iterations are you referring to PCR? | 19:40 |
| @kanzure | it's an iterative process of getting lots of possible aptamers, binding them to your target (if they do at all), then picking the aptamers that work best and using that to inform your next generation | 19:41 |
| BioGuy | hrmm...reminds me a bit of a genetic algorithm, except your evolving something that matches your DNA strand of interest. | 19:43 |
| yashgaroth | that's only really necessary for binding to non-DNA things...DNA binds to itself pretty predictably already | 19:44 |
| @kanzure | yeah what's wrong with a hybridization microarray or something | 19:45 |
| @kanzure | am i making things up again? | 19:45 |
| yashgaroth | microarrays ain't DIY | 19:46 |
| @kanzure | taking pics of microarrays is diy :P | 19:46 |
| @kanzure | but ok i agree | 19:46 |
| yashgaroth | like, I would've said 'just send off the PCR reaction to a commercial sequencing company for $3', but DIY etc | 19:47 |
| @kanzure | that's what mac cowell's company "snparty" or "genelaser" was doing | 19:48 |
| @kanzure | until he realized nobody cared | 19:48 |
| BioGuy | Ok I think I get what an aptamer is, but how would that be useful in identifying what a specific SNP of a gene was? | 19:54 |
| BioGuy | cpopell: Whats the deal with DARPA? | 19:55 |
| yashgaroth | theoretically if you conjugate it to a dye or fluorophore, you can wash it over your DNA sample and it'll stick if you have the SNP of interest | 19:55 |
| cpopell | BioGuy: I started a consulting firm to formalize some side forecasting/analysis work I do, and my friend and I had an idea and asked darpa to give us some cash :P | 19:55 |
| cpopell | not bio related | 19:55 |
| BioGuy | nice! | 19:55 |
| cpopell | I mean, even if we don't get it it gave us some experience | 19:56 |
| @kanzure | by "forecasting/analysis work" he means he uses a giant spreadsheet because he hates technology -_- | 19:56 |
| BioGuy | hehe | 19:56 |
| cpopell | kanzure: I do have a client or two actually :P | 19:56 |
| -!- delinquentme [~asdfasdf@rrcs-74-219-159-104.central.biz.rr.com] has quit [Ping timeout: 255 seconds] | 19:56 | |
| cpopell | one of them even pays me | 19:56 |
| cpopell | ...not much, but it's only a couple hours at a time | 19:57 |
| BioGuy | yashgaroth: So...I'm assuming in your aptamer you would have the sequence flanking your snp? | 19:57 |
| -!- delinquentme [~asdfasdf@rrcs-74-219-159-104.central.biz.rr.com] has joined ##hplusroadmap | 19:58 | |
| yashgaroth | mhm | 19:58 |
| @kanzure | BioGuy: he was right though, it's easier to just do dna hybridization assays in this case | 19:58 |
| BioGuy | Be back in a few, going to grab something to eat. You lot think you'll be around in an hour? | 20:00 |
| yashgaroth | cha bro, you know it | 20:00 |
| @kanzure | we are 24/7 | 20:00 |
| @kanzure | sleep is for the weak | 20:00 |
| BioGuy | lol be back in a bit, this is good talking! | 20:01 |
| -!- jmil [~jmil@hive76/member/jmil] has joined ##hplusroadmap | 20:20 | |
| -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Ping timeout: 248 seconds] | 20:33 | |
| -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap | 20:43 | |
| -!- sivoais [~zaki@199.19.225.239] has quit [Ping timeout: 246 seconds] | 20:44 | |
| -!- sivoais [~zaki@199.19.225.239] has joined ##hplusroadmap | 20:48 | |
| skorket | evening all | 20:48 |
| Mariu | hey | 21:28 |
| -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Ping timeout: 276 seconds] | 21:43 | |
| -!- jmil [~jmil@hive76/member/jmil] has quit [Ping timeout: 256 seconds] | 21:57 | |
| -!- Juul [~Juul@208.87.217.74] has joined ##hplusroadmap | 21:57 | |
| -!- jmil [~jmil@hive76/member/jmil] has joined ##hplusroadmap | 21:57 | |
| -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Quit: Leaving] | 21:59 | |
| -!- Juul [~Juul@208.87.217.74] has quit [Read error: Connection reset by peer] | 22:14 | |
| -!- delinquentme [~asdfasdf@rrcs-74-219-159-104.central.biz.rr.com] has quit [Quit: Leaving] | 22:16 | |
| @kanzure | "And my standard example of an inefficiency of the current situation is that there is good evidence that hookworm is an effective treatment for Crohn's disease, but since hookworm is not patentable, there is no prospect that this treatment will ever get evaluated." | 22:29 |
| @kanzure | what? why doesn't someone just use hookworms then? | 22:29 |
| brownies | where is the evidence? | 22:35 |
| brownies | why doesn't someone open Hookworms 'R' Us? | 22:35 |
| @kanzure | yeah my thought too | 22:35 |
| -!- sivoais [~zaki@199.19.225.239] has quit [Read error: Connection reset by peer] | 22:39 | |
| -!- sivoais [~zaki@199.19.225.239] has joined ##hplusroadmap | 22:40 | |
| -!- cpopell [ad42e490@gateway/web/freenode/ip.173.66.228.144] has quit [Ping timeout: 245 seconds] | 23:07 | |
| -!- nmz787 [~Nathan@pool-108-39-145-80.pitbpa.fios.verizon.net] has joined ##hplusroadmap | 23:12 | |
| @kanzure | nmz787: hi | 23:13 |
| @kanzure | actually, wait, no i'm asleep i think | 23:13 |
| nmz787 | hi | 23:16 |
| nmz787 | oh | 23:16 |
| -!- streety_ [streety@2a01:7e00::f03c:91ff:feae:ded6] has joined ##hplusroadmap | 23:29 | |
| -!- Netsplit *.net <-> *.split quits: streety | 23:29 | |
| -!- streety_ is now known as streety | 23:29 | |
| -!- Mokstar1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap | 23:30 | |
| -!- Skoofoo [~Skofo@adsl-76-197-227-207.dsl.chcgil.sbcglobal.net] has joined ##hplusroadmap | 23:30 | |
| -!- Mokstar [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 244 seconds] | 23:30 | |
| -!- Mariu [Jimmy98@89.41.57.33] has quit [Ping timeout: 265 seconds] | 23:43 | |
| --- Log closed Tue Oct 02 00:00:43 2012 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!
