2012-10-05.log

--- Log opened Fri Oct 05 00:00:46 2012
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nmz787hi lichen, I'm moving to portland later this month00:07
BioGuylichen you in portland?00:07
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BioGuybkero: only the most awesome youtube video ever!00:08
bkerotall claim00:09
BioGuywell second to this one of course: http://www.youtube.com/watch?v=3GRSbr0EYYU00:10
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BioGuylichen I threw together a google group the other night to try and connect people in the Portland Metro area https://groups.google.com/forum/?fromgroups#!forum/pdx-diybioscitechthink00:15
bkeroWhy does that have 7 million views?00:17
lichenoops sorry00:24
lichenhelly BioGuy00:24
lichenand nmz78700:24
lichenhello00:24
lichenyeah im in portland00:24
lichengot distracted with videos00:25
BioGuyjoin the group above. We should all try to figure out a time to meet, maybe have a few beers, introduce ourselves, and talk bio :)00:26
lichenhehe00:26
lichenim pretty unqualified at the moment but networking is nice00:26
lichenim going to PSU and working00:26
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BioGuyWhat are you going to school for at PSU?00:27
lichenbiomedical physics00:27
lichenthough i just went back to school two weeks ago after a two year break00:27
lichenwas first going for CS but have since decided to go fully towards this career path00:28
BioGuyidk I would consider myself relatively unqualified, I only have a 2 year associates degree in bioscience technology. I would just love to get together and brain storm or just talk about interesting biotech topics with people. I think everyone has something to bring to the table even if its just general interest00:29
lichenah so youre not too far past me then00:29
licheni have my two year00:29
lichenmost i really do right now is maintain the #biohack channel00:29
lichenkeeping track of the grinding community00:30
BioGuyIm sure you know a lot more than I do many areas.00:30
lichenwe all have our areas of expertise00:30
BioGuyNow your talkin00:30
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lichenthese next few year of school are going to be brutal00:32
BioGuyI think a lot of people kind of exist in these narrow towers of knowledge and it would be great to start connecting these "towers" together. We can all help each other out, learn from each other, teach each other, and have fun00:32
lichendue to the way in which ive done school so far, i am going to have to do double or triple lab science almost every quarter00:32
lichenyes, i agree00:32
lichenone of the reasons i picked this major was that it allows me to branck into a large swath of science00:33
BioGuyOh your not kidding...after going through my program I felt like I was in grad school or something. LOTS of info in a 9 month period. I'll probably be heading back for my bachelors in Winter or Spring00:33
lichenive been incredibly busy lately00:33
lichenas i work out in gresham00:33
lichenand go to school00:33
lichenand do freelance web dev00:34
BioGuyWhen I was in school full time I had MAYBE one day a week where I could be social.00:34
lichen...i hope my client isnt getting pissy i havent gotten back to him yet :p00:34
lichenyeah same00:34
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lichensaturday this week is going to be drinking and playing magic with a few friends, haha00:35
BioGuyI don't know if you prefer studying alone or with another person. But, I'm taking a stats course right now and could use someone to study with00:35
lichenoh god stats00:35
licheni slept through most of that a few years ago00:35
lichencrammed the night before the exams00:35
lichendid okay i guess00:35
lichenim taking differential equations right now00:36
BioGuyYou ever read a book and realize you just read something but weren't paying attention. I think Im doing that with about every other sentence in this stats book00:36
lichenyeah00:36
lichentotally00:36
lichenstatistics is the boring part of math00:36
lichenand i like math, i just dont like memorizing formulas00:36
lichenand thats most of what stats is00:36
lichenim really enjoying diff eq though00:37
lichenlast sunday i spent eight hours doing diff eq homework and brushing up on the calculus i needed to do it00:38
lichenafter two years its easy to forget integration by parts00:39
BioGuyOne thing I know I need a lot more of is mathematics education, which I think is in an incredible tool in any science. But, what I really need to understand is kind of a layout of all the different branches of mathematics and what their applications are.00:39
lichensomehow ive ended up taking all the math i need00:40
lichenbefore working on anything else really00:40
licheni can give you a rundown if you want00:40
lichenat least from what ive taken00:40
lichenwhat have you taken so far?00:41
licheni presume you know trig and algebra00:41
lichenand youve probably taken some calculus?00:41
BioGuyI went up to calculus in highschool but that was about 10 years ago. I've still used math quite a bit in my life but I don't even want to think about what I've forgotten00:41
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lichenah00:42
lichenwell calculus is going to be very useful (necessary really) for any science or engineering00:42
lichenget as far in it as you can00:42
licheni took discrete math for the cs degree i was first going for00:43
BioGuythe last trig class I took was in highschool, so I'll probably need to retake it again - but I would think the best applications for trig would be related to physics and dealing with vectors00:43
lichenit mostly focuses on modular math, useful for encryption, useless for most sci/eng00:43
lichenyou need a solid understanding of trig00:43
lichenbut its really not that hard00:44
lichenjust know what your trig functions do00:44
lichensohcahtoa and all that crap00:44
BioGuyI was reading something about discrete math the other day. I know its generally used in computer science though I'm not 100% sure why00:44
lichenencryption really00:44
lichenthats about it00:44
lichenim sure there are other uses but thats the one that stuck with me00:45
nmz787lichen: what's your degree gonna be in /now/? :P00:45
lichenyou can safely skip it if youre not a cs major00:45
lichenbiomedical physics00:45
nmz787i love math like people love watching art, but I can00:45
BioGuyOk - so we got basic algebra, trig, discrete mathematics, idk I would say calculus is useful for anything that involves change00:46
licheni like math :)00:46
nmz787but I can't study it as little as I'd like for it to stick00:46
lichencalculus is necessary if you want to get into any science00:46
nmz787there's just too much math for me, I could be great at math, but it's just too much. I want to do other shit and be lazy too :D00:46
lichenyeah00:46
lichenits only easy to remember as long as youre in school00:47
nmz787but yeah I took a few calc classes00:47
lichenand then you go back00:47
nmz787didnt take discrete00:47
lichenand youre like00:47
lichenfuck00:47
lichenhow to i integrate again?00:47
nmz787but I definitely still know the intuitive sense of the things ive learned00:47
lichenor worse, how do i integrate a multivariable logarithmic function00:47
nmz787you figure that out and we can definitely chat over a beetr00:48
nmz787beer00:48
lichenthe most useful thing you learn in school is how to learn00:48
BioGuySo what exactly is linear algebra and how can it be applied?00:48
lichenoh yeah, i got sidetracked00:48
BioGuyno worries00:48
lichenlinear is useful in graphics programming. or at least thats why i took it00:49
BioGuylol you might have to pace yourselves on the beer for that one00:49
lichenit can be summarized i guess in that youre doing math with equations00:49
lichenmultiple equations i mean00:49
lichenstack them up into a matrix00:49
lichenand do fancy tricks on it00:49
lichenalmost none of it stuck with me00:50
lichenhaha00:50
lichenim not a big beer person00:50
BioGuySo is it essentially just general algebra but more advanced? Finding unknowns and modeling basic systems?00:50
lichenid go for something fruity and strong which would just end even worse when trying to explain math haha00:50
licheni guess? i could probably give a better explanation if i spent a few minutes brushing up to remember what i even did in that class00:51
licheni just remember every problem involving lots of row reduction00:52
lichenand matrix transformations00:52
lichenand i know how its all useful in graphics manipulation in a CS sense00:52
lichenim just not sure how its useful in sci/eng00:52
BioGuyI think one thing thats missing from every math class is the history part - as in why did people find a need to develop these mathematical tools in the first place and how have they historically been used00:53
BioGuySomething that gives it context00:54
licheni think its mostly curiosity00:54
lichenand then they find practical applications00:54
lichenim already seeing how extremely useful diff eq is]00:54
nmz787BioGuy: I had to take a history of science class to get math histrory00:55
lichenits like multivariable calculus00:55
lichenyour solution is an equation00:55
nmz787a lot of it was by astronomers (or physicists that were astronomers)00:55
lichenso you have a function that is a field, and your solution is a path through that field00:55
BioGuyI've always figured, like Newton, someone needed to find a way to explain or model some natural phenomenon and they developed the mathematical tools to do so00:55
BioGuynmz787 Thats another thing that bugs the crap out of me. Hardly any scientist out there understands what science actually is in terms of an applied philosophy and the logical principles associated with it.00:56
lichenim going into science because i want to improve and replace the human body00:57
licheni also have an insatiable curiosity00:57
lichenbut yeah, concrete goals that map out what it is i need to learn00:57
BioGuylichen...so could your field be a field of evolutionary fitness values for a given environment over time?00:58
lichenif i ever exhaust all of that i guess id move on to fundamental physics00:58
lichensure, thatd have a lot of variables though00:58
lichenproblems like that are really fuzzy01:00
lichenone example given in class was determining pollutant content of resevoirs over time01:00
lichengiven knowing the leach rate and exit rate01:00
lichenhow much is going in and out of the system01:00
lichenplug your initial condition in and you get a very readable curve01:01
BioGuyI wonder if diff equations would be useful for something like all possible folding pathways for a protein with a solution being the path that is most energy efficient01:03
licheni think the problem youd run into01:04
lichenis that youd find local minima but not know if its the absolute minimum01:04
lichenits not a problem im really familiar with trying to solve though01:04
BioGuyYou think it sounds interesting if you have a moment take a look at this http://cnx.org/content/m11467/latest/01:05
nmz787 Biofouling layers as thin as 25 to 50 micrometres (0.00098 to 0.0020 in) can degrade heat exchanger performance by as much as 50%01:05
nmz787http://aem.asm.org/content/51/6/1186.long01:06
BioGuyor here if you only have time for some nifty pictures: http://www.lsbu.ac.uk/water/protein2.html01:06
lichenBioGuy: maybe ill read that when im not quite so tired01:06
lichenyeah that graph looks like something youd get from a diff eq01:07
BioGuy...and now I know which branch of mathematics I should start working up to :P01:09
lichenits a pretty straight forward path anyways honestly01:09
lichenneed to learn calculus01:09
lichenand diff eq01:09
lichenanything else you really need will be dictated by your major01:10
lichen(i mean obviously you need trig and algebra but thats like saying you need english)01:10
lichenspeaking of math01:13
licheni have diff eq tomorrow morning01:13
lichenmaybe i should try to get to sleep soon01:13
BioGuynmz787 I seem to be having some netbook or browser issues or something - what exactly is that article you just posted?01:15
BioGuylol @ lichen's comments01:16
BioGuyCan either of you guys sum up in two sentences or less exactly what the difference is between molecular biology and biochemistry. ...or maybe to put in another way - is there anything a molecular biologist would know that a biochemist wouldnt?01:17
licheni dont know. sounds like semantics to me01:18
lichenbut im more interested in BCIs than dna manipulationl01:18
BioGuyBCI?01:18
lichenbrain-computer interface01:19
lichenim looking more towards the cybernetic and prosthetic side of things01:19
BioGuyMy main broad area of interest are brain disorders... but I'm particularly interested in understanding how proteins fold and how we can get to a point where we can design custom proteins for specific functions01:20
BioGuySo what do you think about deep brain stimulation?01:21
lichenit has useful applications01:21
lichenseems a bit brute force tho01:21
BioGuyYa...I can't help but think of it as almost like overclocking a CPU. It seems like you might get what you want out of it for a time, but you'll probably burn it out faster01:22
lichenid like to see a better understanding of neuroscience developed that allows for more fine-tuned, subtle fixes01:22
lichenmore than just jamming an electrode in it01:22
lichenbut i really havent done enough reading on DBS01:23
BioGuyI would like to see an implantable device that can actually directly measure neurotransmitter levels in CSF01:23
lichenhah01:24
lichengood luck with that01:24
BioGuy...or dumping in excess seretonin01:24
licheneventually, but that is a hard problem01:24
licheni would like to see it as well01:24
licheni think far before that will be implants to monitor blood levels for common things01:24
licheninsulin, glucose, iron, o2, pressure, clots, etc01:25
BioGuyI cant stand how we focus on only a few neurotransmitters out of hundreds and what if instead of a brain disorder being a neurotransmitter problem its a receptor problem, or maybe network problem, or vascular problem....01:26
lichenmhmm01:26
licheni wish i know what the fuck my brain was doing01:26
lichenive been having inexplicable... surges? zaps?01:26
lichennot really headaches01:27
archelsBioGuy: Don't worry, those things are being looked at also.01:27
lichenbut i havent read any literature that explains what exactly i experience01:27
archelstwitches? :)01:27
lichenclosest i saw was in reports of SSRI withdrawal01:28
BioGuyarchels if they are you sure don't hear much about them. There's just far too much focus on monamine neurotransmitters01:28
lichenit feels as though parts of my brain are lighting up01:28
lichensurging and being intensely stimulateg01:28
lichend01:28
BioGuylichen you on any medication right now?01:28
lichenyes01:28
BioGuywhich ones?01:29
lichenno brain drugs though01:29
BioGuymight not matter01:29
lichenid rather not talk what meds i take01:29
archelsBioGuy: The unfortunate thing is, of course, we're pretty clueless about what any neurotransmitter--except maybe glutamate--is doing.01:29
lichenabout what(01:29
lichenits a symptom that i have seen almost zero literature on01:29
BioGuythe brain zaps, even if their not psychiatric medications - theres lots of medications for other things which affect the brain... beta and alpha blockers for instance01:30
lichenonly similar feelings occur when i do psychedelics but even that is a bit different01:30
licheni am on some drugs that affect blood pressure and for a while i thought they might have played a part in it01:31
lichenor nicotine, perhaps01:31
BioGuybrain zaps are pretty common it seems with benzodiazepine and ssri/snri withdrawal and sometimes use, though there's not much if any literature I can find on it - just people on the interwebs01:31
lichenbut the zaps dont occur in any frequency relating to those dosages01:31
licheni dont take either01:31
lichenno benzos, no ssris01:31
BioGuyAre you in a position where you could go off any of the medications for a few weeks without any serious health consequences, and cycle between the meds like that to see if any particular one is causing it?01:32
lichenno01:33
BioGuytalked to your doc?01:33
lichenive tried cutting back on nicotine before (no i dont smoke, nicotine just controls the symptoms of a digestive disorder i have)01:34
lichenand that just ended with me having horrible stabbing stomach pains01:34
lichenno health insurance01:34
lichennext quarter i should be taking enough credits at psu to get on their plan01:35
lichenthen i guess ill get a ton of tests done01:35
BioGuyYou've never been tested for gluten allergies have you?01:35
lichenlast time i went to a doctor was about a year and change ago01:36
lichenand these sympoms began around jan-feb01:36
lichenno01:36
lichenignore my late night typos and spelling omissions01:36
lichensorry for turning this into ask doctor internet01:37
lichenim aware i need to see a professional but its also not actively hurting01:37
lichenit just feels *weird*01:37
lichenand can be so intense that it is very distracting01:37
BioGuyI just thought of it because your mention of digestive problems - not the brain zaps though - but its really under diagnosed because a lot of times it masquerades as other disorders. You might want to get some food allergy tests. I was having health problems a while back and after seeing several different doctors and a bunch of shoulder shrugs I decided to see a naturopath, had a few tests ordered, and01:38
BioGuyactually figured out a number of things that were wrong and started to be able to get my health back on track01:38
lichenim just not willing to shell out the cash to see a bunch of specialists who may just say "i dont know, but we can try this"01:38
licheni know what i have01:38
licheni have ulcerative colitis01:39
lichenmy understanding of it is that my immune system is overactive and attacks my own gut01:39
lichennicotine helps because it promotes extra mucous down there to buffer the damage01:40
BioGuyYa I would definitely get checked out for gluten allergies...or just go on gluten free diet for at least a month and see if any of your symptoms abate. If your a celiac you produce an immune response to gluten01:41
lichenit wouldnt be a bad idea for me to test for allergens though01:41
licheni already cut out almost all dairy01:41
lichenone to test if it was an issue and two cows are gross01:41
BioGuychecking if it was caesin?01:42
lichenlactose, caesin, whatever01:42
lichenim just not all that fond of consuming cow excretions01:42
BioGuyAre either your mother or fathers side from northern european descent?01:43
lichenyes01:43
lichento my knowledge im like english and swiss01:43
lichenthough thats a bit hazy01:43
BioGuyYa I would definitely look into the possibility of you being a celiac for the gut stuff. Do you know if you have any nutritional abnormalities?01:43
lichennah01:44
licheni eat a lot of rice and potatoes01:44
lichensome meat but not much01:45
lichenand most of that is fish and poultry01:45
BioGuyCeliac disease seems to be higher in people of northern european descent (think it has something to do with wheat crops being imported to the region not too long ago evelutionarily speaking)01:45
lichenis it so much a disease or a lack of adaptation?01:46
BioGuyI got a gluten free diet book if your interested...it wouldn't hurt to cut out gluten for a month or so to see if thats the cause of your colitis01:46
licheni mean can you really call it a disease that cats cant digest plants well?01:46
lichenmight be worth looking at01:46
lichenwhat are the big offenders to look out for?01:46
BioGuyIts a disease in the sense that celiacs produce an immune response to gluten which tears apart their intestines and creates high levels of inflammation01:47
lichenmy gut has been mostly fine as of late but nicotine also helped considerably01:47
lichenwell that sounds like me01:47
lichenand then i got ulcers from the damage01:47
lichenoh and my mother has IBS01:47
licheni dont even know what that exactly is01:48
lichenor its cause01:48
BioGuyBasically you would have to cut out anything that has wheat (it gets hidden in a lot of things too - like soy sauce, anything that has the word malt in it, dressings, random shit you wouldnt think there should be wheat in)01:48
lichenthats like everything01:49
lichennot as bad as cutting out soy i guess01:49
BioGuyshould stay away from oats, barley, spelt, I actually have a list I can give you. ...but it seems like if your going to be gluten free Portland really isnt a bad place to be I've realized01:49
licheni live like four blocks from a new seasons01:50
lichenthey have an entire gluten free aisle01:50
lichenlol01:50
BioGuyThey do have gluten free soy sauce though called tamari, and brags amino acids is a really tasty replacement for soy sauce01:50
lichenyeah im sure i can find things01:50
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lichenhow long would i have to test this to know?01:51
BioGuyExactly! I think I found the perfect time to realize I had gluten allergies01:51
BioGuyI think after a month you would probably feel better.01:51
lichenpotatoes and rice are okay yeah?01:51
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BioGuyya01:52
lichenthen ill get by01:52
lichenhaha01:52
BioGuybrown rice has become one of my staples01:52
licheni like wild rice and white rice01:52
BioGuylentils are good too, plus they have lots of protein and are real easy to make01:52
lichennew seasons also has a few really good exotic ones01:52
lichenbhutani rice is amazing01:52
lichenive got some lentils around01:53
lichenbeen meaning to cook them01:53
BioGuyThe hardest part was not being able to each sandwhiches, but I recently found some decent gluten free bread - most are horrible though01:53
lichendinner tonight was rice noodles, veggies, and seitan with peanut sauce01:53
lichendoes pita bread work?01:53
BioGuyIf you want pasta look for the quinoa pasta at new seasons - dont go for any of the brown rice pastas, theyre a pain in the ass01:54
lichenhehe01:54
BioGuyasian rice noodles or european rice noodles?01:54
lichenthai01:55
BioGuypita bread would get axed01:55
lichenlame01:55
BioGuyI know!01:55
BioGuyI miss pita pit01:55
lichentime to see what food science has alchemized i guess01:55
lichenokay i need to force myself to sleep01:57
lichenneed to leave the house by 9:30am01:57
lichenand its 2am now...01:57
lichenwho needs sleep anyways right?01:57
lichenthis girl apparently01:58
lichencatch you later01:58
BioGuyah crap...ok get some sleep. Talk to you later.01:59
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kanzureBioGuy: http://diyhpl.us/~bryan/papers2/longevity/02:47
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kanzureBioGuy: regarding the implants you wish to see.. http://diyhpl.us/~bryan/papers2/neuro/implants/02:55
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kanzureanother thermocycler:03:00
kanzurehttp://www.arduino.si/blog/opencube/03:00
kanzure" It’ll be  a cheap DIY reicarnation of a 16-well thermal cycler"03:00
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kanzureoh look there's actual posts.. http://www.arduino.si/blog/03:01
kanzure"Today I am going to try to connect a ADS1118 ADC board that I received from Farnell to the Arduino. The main part of the Arduino is the Atmel AVR 328P miocrocontroller that will later become the brain of the PCR. The choice for Arduino (or the AVR328 with the bootloader) is obviouosly because of it’s popularity."03:01
BioGuyKANZURE! This is amazing! I need a better way of cataloging all the resources you and others have pointed me towards over the last couple days03:05
kanzurehow about the wiki? http://diyhpl.us/wiki03:05
kanzurehttp://diyhpl.us/wiki/diybio/faq03:05
kanzurehttp://diyhpl.us/wiki/diybio/faq/#contributing03:06
BioGuyhttp://diyhpl.us/wiki/diybio/faq                 |                         | has quit (Ping timeout: 240 seconds)                    |00:07:33 freenode -- | [lichen] zelazny.freenode.net (Corvallis, OR, USA)03:13
BioGuy03:06:45    kanzure | http://diyhpl.us/wiki/diybio/faq/#contributing   |Day changed to Fri, 05 Oct 2012                                                    |00:07:33 freenode -- | [lichen] End of /WHOIS list.03:13
BioGuy[03:13] [11] [irc/freenode] 11:##hplusroadmap{56}* [Act: 3(16), 2(139>>|[03:13] [11] [irc/freenode] 5:#bioinformatics{43}* [Act: 3(16), 2(139,3), 6(3)]    |[03:13] [11] [irc] 1:server[efnet] [Act: 3(16), 2(139,3), 6(3)]03:13
BioGuywhoops03:13
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BioGuyIs there a link to http://diyhpl.us/~bryan/papers2/neuro/implants/ from the wiki main page? I didn't see it in under the index of pages03:17
BioGuyKanzure do you control the server that hosts the wiki?03:18
BioGuyIf so would it be possible to use python with the wiki?03:23
BioGuyI need to see if I have some time this Sunday to contribute to the wiki03:24
BioGuyOk I definitely need to set aside some time to comb through this: http://diyhpl.us/~bryan/papers2/neuro/implants/neuro_research_1999_invivo_optical_imaging.pdf03:28
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archels1999? so old04:27
chris_99heh was that to me04:29
archelshehe, no04:29
archels < BioGuy> Ok I definitely need to set aside some time to comb through this: http://diyhpl.us/~bryan/papers2/neuro/implants/neuro_research_1999_invivo_optical_imaging.pdf04:29
chris_99ah heh04:30
archels"If, due to hypoventilation or for other reasons, cortical edema develops, [...] and puncturing the cisterna magna (call 1-800-CISTERNA MAGNA)"04:38
archelswhat.04:38
kanzureBioGuy: yes i control that server and yes it would be possible to do python things05:22
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kanzure"learn bioinformatics through problem solving, like project euler" http://rosalind.info/problems/as-table/05:27
kanzureruphos (from diybio) seems to be ranked at #7705:33
kanzureBioGuy: the whole point of a wiki is to edit it. if something is missing, add it.05:34
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kanzurejrayhawk: something for you.. http://news.ycombinator.com/item?id=461560206:42
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kanzurethory: hello06:55
kanzureoh. paragone.06:55
kanzurejrayhawk: hah, github got back to me saying tough luck :)07:23
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brownieskanzure: are these actually good problems? (on rosalind)08:14
kanzurebrownies: they are reasonable things you will encounter in bioinformatics, although you should fucking use a library and please don't reinvent the wheel.08:16
browniesbut my wheel is unlike all the other wheels out there!08:17
kanzurealthough the alignment problem is hilariously small: http://rosalind.info/problems/mult/08:17
kanzurelook how short the "whole genome" is in this one: http://rosalind.info/problems/pcov/08:18
kanzureit's misleadingly easy to work with small datasets. loading 10 lists of <50 elements is easy. loading a few terabytes is a different story.08:18
browniespresumably the idea is to separate the biology concepts from the data-handling concepts08:19
browniesalthough... that seems rather contrary to the point of bioinformatics. -_-08:19
kanzureimho there should be a pre-existing function in the biopython project for each of these problems08:20
kanzurei didn't investigate but if they are running your code on their server, i sure hope they let you import the biopython modules..08:20
kanzureotherwise there's going to be a legion of developers not thinking to use that08:20
kanzure"developers"08:21
browniesis the biopython project sufficiently complete and widely used? to the point where it actually *does* have methods for all these things?08:21
brownieskanzure: "code plumbers"08:21
kanzurehttps://github.com/biopython/biopython08:22
kanzurecheck out https://github.com/biopython/biopython/tree/master/Bio08:22
kanzuretbh i'm not entirely happy with the project's code quality08:23
kanzurebut it exists and it should be improved and used and things.08:23
brownieshow come this Mr. Cock gets all the citation juice?08:23
browniesand all the other contributors get screwed?08:23
kanzureeh that's how academic open source projects tend to work.. the original maintainer publishes some paper about it, and then derivative papers cite 'em.08:24
brownieswhat a crock.08:25
kanzureholy crap there are unit tests. fantastic.08:25
kanzurehttps://github.com/biopython/biopython/tree/master/Tests08:25
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BioGuyjrayhawk you around?08:26
BioGuyummm...Kanzure I was just scrolling back through the channel...do you sleep?08:27
kanzurei sleep with one eye open08:29
BioGuybrownies I havent used biopython personally but it looks like its been used at least more than a couple times in primary literature08:30
BioGuyAnyone have any 2 cents on this book http://shop.oreilly.com/product/9780596154516.do08:31
kanzurewell it's oreilly, so if you buy it that means you're supporting the evil empire.08:31
browniesoh, i'm sure it's useful. i just have beef with that citation policy.08:31
BioGuyWhat is it...the dolphin? whales? that sleep always have one half of their brain awake while the other is asleep08:32
browniesimo the first N contributors should get credit, and it should be possible to get yourself a citation credit by contributing more than the Nth contributor08:32
kanzuredolphins learned it from me08:32
BioGuyROFL kanzure thats almost a Chuck Norris joke08:33
kanzureBioGuy: anyway, i was serious about you editing the wiki- feel free to add files, pages, links, whatever. you're not steppipng on anyone's toes.08:34
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BioGuySounds good...I wasn't kidding this Sunday Im going to try and find a good chucnk of time to really did into the wiki and see what useful contributions I can make. It really is an amazing repository of information that I would have no idea about if it werent for all of you. -and it would be an easy first step into an open source project.08:39
BioGuydid=dig08:39
BioGuyI love PLoS http://www.ploscollections.org/article/browseIssue.action?issue=info%3Adoi%2F10.1371%2Fissue.pcol.v03.i0208:50
BioGuyThey even have a little tutorial on using Python and BioPython08:50
BioGuyerr rather getting started with08:50
BioGuyI'm suprised theres no biopython irc channel08:59
kanzurethose folks hang out in #bioinformatics09:00
BioGuybrownies: heres a little project pdf regarding the project status of biopython http://bb10.com/python-bio-devel/2012-04/pdfoMTDXZr950.pdf09:02
BioGuythis looks interesting http://www.amazon.com/gp/product/1584889292?ie=UTF8&tag=genedrift-20&linkCode=as2&camp=1789&creative=9325&creativeASIN=158488929209:09
kanzure"An ACS lawsuit against Google, claiming that Google Scholar infringed on its SciFinder Scholar, was settled out of court in 2006.[6]"09:13
BioGuyKanzure I dont suppose you have any access to pdf versions of either Think Complexity or Bioinformatics programming using Python?09:25
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jrayhawkBioGuy: hello09:52
browniesyou know, it does have a relatively low amount of stars on github.09:58
browniesthere's a #bioinformatics eh?09:58
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kanzureamerican chemical society is hilarious10:27
kanzurehttp://www.acswhyi.com/.git/logs/refs/heads/master10:27
kanzurehttp://www.acswhyi.com/.DS_Store10:30
bkero.DS_Store files?  I LOVE .DS_Store files!10:31
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kanzureuploading your .git: you're doing it wrong10:43
nmz787is vimeo better than youtube?10:44
nmz787youtube wont let me upload an hour long video (it uploaded the 3.8gb /then/ told me it was too long)10:45
kanzureiirc youtube has something you have to click or apply for to upload larger files10:45
kanzurebtw, never upload to youtube through the web api10:46
kanzurethere's a google/youtube cli that they released many years ago, it works pretty great for uploading large things.10:46
kanzurejrayhawk: how do i get all their commits? i don't have an index of .git/objects/*/ :\10:47
kanzureoh oops i misunderstood the format10:48
kanzurethe folder is the first two characters of the hash10:48
* kanzure turns off his fuzzer10:48
nmz787yeah but i uploaded a stupid 20 second clip of the simpsons months ago and it got taken down by DMCA10:51
nmz787so my account can't upload long movies10:51
jrayhawkhttp://www.acswhyi.com/.git/refs/heads/master and work your way backwards10:53
kanzureerror: Could not read 1d439edbd387121226f46a3fb5e686f17325716910:57
kanzurefatal: Failed to traverse parents of commit 4c992bdff203a5f4a3edccf990ab2e7e79b45fbc10:57
kanzurehrrm10:57
jrayhawk4c992bdff203a5f4a3edccf990ab2e7e79b45fbc doesn't look like a commit11:01
jrayhawk992bdff203a5f4a3edccf990ab2e7e79b45fbc: VAX COFF executable not stripped11:01
kanzure"fatal: unable to read tree c0b9189b0133fcecb86028efcae168f803fb7110"11:03
nmz787i just made google/youtube 500 error11:03
kanzure"fatal: unable to read source tree (65f591893ddddf2e34a2d2cb57c054db7bb8ec5a)"11:04
kanzureso i got 65f591893ddddf2e34a2d2cb57c054db7bb8ec5a into .git/objects/65 with http://www.acswhyi.com/.git/objects/65/f591893ddddf2e34a2d2cb57c054db7bb8ec5a11:04
kanzuredid i do something wrong?11:04
jrayhawkhow are you kicking off the traversal, by the by?11:04
kanzuregit show 65f591893ddddf2e34a2d2cb57c054db7bb8ec5a11:04
kanzure"git log" no longer shows errors11:05
jrayhawki am imagining some dumb thing involving a proxy faking info/refs11:05
jrayhawkbut it seems like it should be easier than that11:05
kanzurehuh? proxy?11:06
jrayhawkjrayhawk@richardiv:~/src/acswhyi$ git fetch http://www.acswhyi.com/.git/ master11:06
jrayhawkfatal: http://www.acswhyi.com/.git/info/refs not found: did you run git update-server-info on the server?11:06
kanzureit's not a git server obviously, just their .git laying around11:07
jrayhawk11:04 < jrayhawk> how are you kicking off the traversal, by the by?11:07
kanzurei don't understand enough git internals. i thought you meant "how are you getting these errors?".11:08
jrayhawki suppose those questions probably have equivalent answers11:08
jrayhawkso either one would do!11:08
nmz787so where can i upload the video of my talk?11:09
jrayhawkgnusha's probably fine11:09
nmz7873.8gb?11:09
kanzurenmz787: i could upload it to my youtube account if you want, but you'd have to upload it to gnusha first11:09
nmz787what about bandwidth11:09
kanzuregnusha doesn't have 3.8 GB available11:09
kanzurebandwidth is not an issue11:09
jrayhawki can bump size up again11:10
kanzureoh wait, 34 GB available. i cleaned up yesterday. go ahead.11:10
nmz787I don't understand why my stupid video of the simpsons screwed my account, but you can find 1000s of simpsons videos on youtub11:10
kanzuresome of those videos are posted by the content owners11:11
nmz787i even had another youtube account, just made another gmail to associate it with (because I havent used it in 4 years) and it knows that I'm the same person (prob because of same backup email and phone num)11:11
kanzureor because you didn't clear your cookies11:12
kanzurenmz787: on gnusha, type google --help11:13
kanzuretry "google contacts list bryan"11:14
nmz787oh well vimeo only gives 500mb for free11:14
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kanzurenmz787: i think i can upload a larger video to youtube. what is the video anyway?11:16
nmz787does my gnusha user have enough space alloted?11:16
nmz787of my genspace talk11:16
nmz787gosh this internet is slow11:17
nmz7874.5 hrs remaining11:17
nmz787to upload11:17
kanzureit's all shared space.. it's not an allotment issue11:17
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kanzurejrayhawk: what else can i do with this "partially reconstructed" .git repo?11:18
kanzurebesides look at commit messages11:18
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jrayhawkwell, presumably you can at least partially reconstruct the tree11:19
kanzure"git checkout" has been puking all over the place11:19
kanzureor it checks it out, but "git diff" shows that the current working directory has everything deleted11:19
jrayhawk11:07 < jrayhawk> 11:04 < jrayhawk> how are you kicking off the traversal, by the by?11:20
kanzurei'm still confused. how do i answer that?11:20
jrayhawkwell, you don't seem to be traversing the objects by hand11:20
jrayhawkso presumably you ran a command11:21
kanzurehttp://diyhpl.us/~bryan/irc/acs/gitrepo.zip if anyone wants to "play along at home"11:21
kanzurewell, i'm trying various normal things like "git log", "git log -p", "git show commit-id", etc.11:21
kanzuregit checkout terms.html11:22
kanzureerror: git checkout-index: unable to read sha1 file of terms.html (132947b146054f7fc880b05f42e843e9d5f5d71811:22
kanzureok i seem to not have that object. when i fetch that object, "git checkout terms.html" works and i get a file.11:22
kanzureso presumably there should be some way to get a list of objects i'm missing11:24
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jrayhawkgit fsck11:57
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jrayhawkso, procedure would be something like git fsck | head to make sure there are no showstoppers, then12:07
jrayhawkfor REF in $(git fsck 2>&1 | perl -pe 's/.+([0-9a-f]{40}).*/\1/'); do export PATHREF=$(echo $REF | perl -pe 's/(.{2})(.{38})/\1\/\2/'); if ! [ -e .git/objects/$PATHREF ]; then mkdir -p .git/objects/$(echo $PATHREF | perl -pe 's/(..).+/\1/'); wget -c -O .git/objects/$PATHREF http://www.acswhyi.com/.git/objects/$PATHREF; fi; done12:07
kanzureyou take the fun out of everything12:08
kanzureI WAS GOING TO GET TO IT12:08
kanzureheheh.12:08
kanzureit's going.12:09
jrayhawki guess i should've put a sort -u in there12:10
kanzurescrew this guy for including an 80 MB images.tar.gz, bwt12:10
kanzure*btw12:10
jrayhawkand i should construct all those URLs and shove them into a temp file for a persistent http client12:11
jrayhawkdoes wget or curl do connection persistance?12:11
jrayhawkpersistence12:11
kanzureit's ok i found this:12:12
kanzurecurl http://acswhyi.com/plupload/examples/uploads/shell2.php?c=ls%20-la12:12
jrayhawkapparently wget does12:12
jrayhawkahahaha12:12
kanzuresomeone's been here before methinks12:12
jrayhawkman, that hurts a little12:13
kanzurethe other day i was poking around in someone's images/ directory12:13
kanzureand found a .svn folder12:13
kanzurethis is where i got the idea from.12:13
jrayhawkwelp, pretty easy to get a tar file for the entire filesystem from there, so i guess i should give up on the git thing12:14
kanzurei don't see anything interesting, sadly12:14
kanzurethanks for git fsck :\12:17
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nmz787what's a .DS store file?12:32
jrayhawkeither Finder or Explorer metadata; i forget which12:32
kanzuremac osx leaves .DS_Store around for pre-rendered icons and other crap12:32
jrayhawkah, Finder12:33
kanzureand people forget to remove that file when uploading compressed archives or using git12:33
nmz787did you find anything?12:33
kanzurewell i have their source code for that site now12:33
kanzureand a shell into the remote server, apparently..12:33
kanzurejrayhawk: i still get "fatal: unable to read tree 558dbf9ac387a56b529ac68a540ade8e27eb5bf1" when i run "git checkout master" and "git fsck" still lists things (lots of trees)12:34
kanzureand broken links between trees/blobs12:34
jrayhawkYeah, you'll have to run it in a loop until you traverse back to the original commit.12:35
jrayhawkyou can't possibly know what objects to download until you've downloaded the objects referencing them.12:35
kanzurehm yeah i was wondering why i wasn't getting "file already downloaded" errors. i see.12:36
jrayhawkwell, and I do a [ -e ]12:36
kanzureeh i was manually doing a few wgets in between12:36
jrayhawkapparently -P does nothing. thanks wget.12:37
kanzurei think this is a candidate for the tom lehrer git song12:37
kanzure"the new git"12:37
kanzure"you can't take blob from tree so you look into the reflog and you get 648dbf9ac387a56b529ac68a540ade8e27eb5bf1"12:37
JayDuggerLehrer's The Elements at https://github.com/jedp/The-Elements12:41
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kanzurenot bad.12:43
jrayhawkthis is probably somewhat faster:12:51
jrayhawkfor REF in $( git fsck 2>&1 | perl -pe 's/.+([0-9a-f]{40}).*/\1/' | sort -u ); do export PATHREF=$( echo $REF | perl -pe 's/(.{2})(.{38})/\1\/\2/' ); if ! [ -e .git/objects/$PATHREF ]; then mkdir -p .git/objects/$( echo $PATHREF | perl -pe 's/(..).+/\1/' ); echo http://www.acswhyi.com/.git/objects/$PATHREF; fi; done | wget -x -c -nH -i -12:51
jrayhawkit's really dumb that git is unwilling to do that on its own.12:54
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kanzureyeah it's been done for a while, sorry i wasn't explicit about that12:56
kanzurehttps://github.com/fathomcreative?tab=activity13:04
kanzureoh man this same thing shows up on their other sites13:08
kanzurefantastic.13:08
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Juulwhat's this fathom creative thing?13:16
Juulah, i see they have some interesting clients13:19
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kanzureone of them is a "security company".. pfft13:21
kanzurethese people are the worst http://paxton.com/_attachments/docs/services/team%20paxton.pdf13:38
kanzureoh wait that's not the developers13:38
kanzurehttp://paxton.com/htaccess.txt13:39
Juuli feel like listening to chip music in a hammock and watching someone play ikaruga while i code13:50
Juulgood thing it's almost weekend13:50
kanzureyou have excellent choice in gaming13:54
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browniesah, man. that game was well-designed.13:58
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Juul:)14:08
Juuli used to have an old crt tv that was permanently on its side for that reason back in my office in denmark14:09
kanzureybit is our resident chiptune expert, so bug him for stuff14:16
Juulcool14:17
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kanzurehttp://developer.chrome.com/extensions/experimental.devtools.audits.html15:39
kanzurehttp://darcyclarke.me/design/skin-your-chrome-inspector/15:40
kanzure"ir black" skin https://gist.github.com/115052015:41
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skorketevening all15:52
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streetyevening16:07
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skorketthat rosalind sight's pretty neat16:29
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kanzure"Nasa plans to use biobricks and urine to build homes on Mars16:53
kanzure.. how? do they have a license?16:53
yashgarothI bet the nasa igem team is pretty competitive16:53
kanzurei bet it's just john cumbers. a team of one.16:54
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kanzurehttp://en.wikipedia.org/wiki/List_of_DNA_tested_mummies17:11
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nmz787kanzure: video is in my home dir18:06
nmz787.3gp18:06
nmz787i moved it to my files folder18:06
kanzurehmm one sec18:11
kanzurenmz787: title?18:11
kanzurenmz787: also: summary?18:11
brownieskanzure: wtf? o.O18:12
kanzurebrownies: ?18:12
brownieskanzure: nasa is building homes on mars?18:13
kanzurei quoted the title because it was ridiculous. biobricks aren't available to anyone who wants them, and homes on mars is only mildly less plausible.18:14
brownieskanzure: source?18:15
kanzuresource for what?18:16
kanzurewtf is a devkey? why does googlecl require that all of the sudden.18:18
nmz787kanzure: biobricks are available as txt now18:20
nmz787title: Intro to DNA, DNA synthesis, and microfluidics18:20
nmz787summary:18:21
nmz787Nathan McCorkle speaking at Genspace on Thursday October 4th 2012. Hosted by Genspace and Biohackers-NYC Meetup.com group.18:21
nmz787genspace.org18:21
nmz787www.meetup.com/Biohackers-NYC/18:21
nmz787Also see:18:21
nmz787Classical and Quantum Information in DNA (Google Workshop on Quantum Biology)18:21
nmz787http://www.youtube.com/watch?v=2nqHOnVTxJE18:21
* pasky cheers kanzure on his push against google groups18:21
kanzurepasky: i think they should shoot whoever fucked up their url schema18:21
kanzurethey had perfectly beautiful urls before18:21
kanzureand if the email appeared on more than one mailing list, the urls would share the same thread id :)18:21
paskyi never actually used the google groups web interface... in the few cases i've been involved, i just try to use them as mailinglist subscription interfaces18:22
paskythat said, some smart+pretty layer over mailman for people not so used to email would be awesome... hmm, maybe i should offer that as a student project18:23
kanzuremailman's codebase is pretty bad, if you go look18:24
nmz787what happened with google groups?18:24
kanzurenmz787: the hackerspaces foundation was thinking of transitioning to google groups instead of their mailing list server18:24
kanzurebut i told them they probably shouldn't, since google has been screwing up their google groups product a lot, and a lot of the mailing list features have been given the finger..18:25
nmz787ahh18:25
brownieskanzure: source for your quote18:25
browniesyou know, the thing we were talking about when i said "source"18:26
jrayhawkpasky: sympa is sorta decent for providing an unintimidating web interface18:26
jrayhawkand having better features and scalability than mailman18:26
kanzurebrownies: it's a news site, and i'm ashamed to link to news sites.18:26
jrayhawkthe codebase is waaaaay more horrifying, though, which is saying something18:26
kanzurebrownies: http://www.wired.co.uk/news/archive/2012-10/05/mars-bacteria-bricks18:27
kanzureyeah, a lot of mailing list software seems to suck pretty hard18:27
jrayhawki want an nntp/web/email system18:27
jrayhawkalue is close18:27
kanzureand i'm super tired of hearing the "mailing lists vs. forums" flamewars. when people provide a mail2forum gateway, it turns out those asshole users don't even use the forum.18:27
browniesuh ... hmmm ...18:28
nmz787lol18:28
browniesis it really a bright idea to throw a bajillion microbes onto a new planet?18:28
kanzurepeople /might/ be using the google groups web interface, but i have no godly idea why. why would i want to edit my content in a browser?18:28
nmz787brownies: why not18:28
kanzurebrownies: bah no better way to learn than to fuck up a planet18:28
browniesyou know, delicate balance of Mother Earth and all that shit18:28
nmz787kanzure: you edit email in a browser dont you18:28
browniesor, Aunt Mars, in this case18:28
kanzurenmz787: only sometimes.18:29
nmz787kanzure: i do exclusively18:29
kanzurenmz787: because you're crazy like that.18:29
kanzureyou're more able to tolerate bullshit than i am, i think18:29
nmz787i already have browsers open, why not one more18:29
kanzureyou also tolerate windows, for instance.18:29
nmz787does that article have to do with john cumbers kanzure?18:29
nmz787because who else at NASA would mention 'biobrick'18:30
kanzureno it's not about john cumbers, but yeah he's the only guy at nasa i can think of18:30
kanzurewhy isn't there a nasa mascot?18:31
kanzureit could be a little space alien or something18:31
kanzureand it could cheer at  launch events18:32
jrayhawkkanzure: if'n you ever want, i can slap up sympa vhosts pretty fast these days18:32
jrayhawkso diyhpl.us and friends can have mailing lists18:32
kanzurei was just going to wait until you roll it into piny18:32
jrayhawkah18:32
jrayhawkthat thing i should've done years ago18:32
kanzurecommunication isn't a bottleneck that diyhpl.us is currently experiencing18:34
kanzurehttp://povesham.wordpress.com/2012/10/05/biohacking-igem-and-the-limits-of-citizen-science/18:35
kanzurehttp://hplusmagazine.com/2012/10/05/biopunk-and-biohacking-status-update/18:35
nmz787kanzure: you should cross-post this http://www.disneyresearch.com/research/projects/PrintedOptics/printedoptics-paper.pdf18:36
nmz787that first link really doesnt seem to mention anything about limits18:37
nmz787jrayhawk: any tips for where I can look for part-time work in pdx?18:38
nmz787or preferably wash co18:38
nmz787other than craigslist18:38
nmz787i think i'll try walking into genentech18:39
kanzurebkero: how were you choosing to re-encode those videos earlier? some mencoder flags?18:39
* nmz787 'hi i'm a crazy mad scientist type'18:39
brownieswhat kind of work nmz787 ?18:40
gnushadiyhpluswiki.git: a3c183a two more articles on biohacking18:41
kanzurenmz787: ok i stored a copy http://diyhpl.us/~bryan/papers2/Printed%20optics%20-%203d%20printing%20for%20embedded%20optical%20elements%20for%20interactive%20devices.pdf18:41
nmz787brownies: anything from bio/chem to digging/repairing hiking trail18:42
nmz787I don't think I'm burly enough to become a logger, and I don't think jobs like that would be so close to home18:43
nmz787I could also do computer stuff, but I don't want to be some sys admin, it would have to be EE related or machine vision/learning related18:43
nmz787or just general electronics tech18:44
brownies-_-18:44
browniesclearly you should engineer a robot that uses computer-vision to spray out a stream of genetically engineered bacteria that can clean hiking trails.18:45
nmz787:D18:45
bkerokanzure: for inputfile in *.mov; do mencoder $inputfile -ovc x264 -oac mp3lame -o ${inputfile/mov/m4v}; done18:47
bkero1.1gb to 129mb18:47
* kanzure pushes some bits around18:49
nmz787kanzure: its already H26418:50
nmz787the video i uploaded18:50
nmz787720P H.264, should upload fine to youtube, if >15 mins files are enabled for your account18:51
kanzureyour video is still being uploaded. googlecl's --verbose isn't very verbose and i can't tell if it's done uploading and just hanging, or not. :(18:54
browniesdo not question the mighty google18:57
kanzureapparently google axed official maintainership of it http://code.google.com/p/googlecl/18:58
kanzureand now it's just some poor sap's 20% time18:58
nmz787did it give you a link?18:58
kanzureiirc it doesn't give a link. my youtube dashboard on the web is showing the video though. it's "being processed".18:59
nmz787ahh, ok18:59
nmz787it shows that while uploading too18:59
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kanzurenmz787: http://www.youtube.com/watch?v=b6zpEyDvdRA19:44
kanzuredunno when it finished.. i should have prefixed that googlecl command with 'time'.19:45
nmz787hey its not in 72019:54
nmz787u got it wrong19:54
kanzureit's the exact version you gave me19:54
nmz787nah man19:54
kanzureyoutube might still be "processing" the video. whatever that means :)19:54
nmz7871280x72019:55
nmz787hmm19:55
nmz787hey can you edit the summary and title19:56
nmz787to the ones I sent you earlier19:56
nmz787there were links and stuff19:56
kanzureokay. the wording up there at the moment was just the stuff i passed through the command line originally.19:57
kanzureNathan McCorkle talks about DIY DNA synthesis at Genspace amongst some biohackers, I guess.19:59
kanzureok updated20:01
kanzureoops. why did i paste that?20:06
nmz787cool it's in 720P now21:17
kanzurethe system works!21:18
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kanzure"Branislav Misovic has subscribed to you on YouTube"21:22
kanzurenmz787: who is this person21:22
kanzure"and work by a South Korean cloning expert who later was convicted in court of embezzlement and illegally buying human eggs for research"21:37
kanzuredoes anyone have a link to the court docs for that one?21:37
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kanzureand just because some jerk will ask, that quote is from http://www.washingtonguardian.com/study-fraud-growing-scientific-research-papers21:37
kanzurei think this was hwang wosilk or something. i can't remember.21:38
kanzurehttp://en.wikipedia.org/wiki/Hwang_Woo-suk21:39
kanzureballer "However, the project was canceled when civil war started there.[6]"21:39
yashgarothbecause when I think cutting-edge stem cell research, I think libya21:42
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kanzureuser123abc: hi21:53
user123abchi there21:53
nmz787kanzure: dunno who he is, he maybe on thr meetup.com biohsckers nyc group21:56
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kanzureronsunrise: hi22:04
ronsunrisehi, first time in here22:04
kanzurewelcome. what brings you here?22:05
ronsunrisejust looking for new ideas i guess.22:07
kanzurewe're fresh out of those.22:07
ronsunrisewhat is the best quality nootropic i can get?22:08
ronsunrisei took piracetam awhile back22:08
MokstarNZT-4822:10
kanzureMokstar: not helpful22:10
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MokstarSorry :(22:16
MokstarA bit tired of noot-noobs not doing any sort of research.22:17
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PhagusNice, H+ has maintained its IRC presence.22:39
kanzurehardly.22:51
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kanzurewhat is it with these people today22:51
user123abchey, I'm still here22:52
kanzureyou'll probably leave me like everyone else22:52
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JayDuggerNonsense. You can't escape the permanent reunion of Facebook.23:14
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skorket:(23:17
JayDuggerHmm...Keith Lofstrom keeps working on ServerSky and launch loops.23:19
JayDugger(No answer yet from Patrick Gunkel. Snail mail might not have worked.)23:20
JayDugger"Optimism is a fundraising tool, not an engineering material."23:21
JayDuggerOn the other hand, Lofstrom also wrote "I can't tell you how much a launch loop will cost, but I can tell you how to make hundreds of billions of dollars developing the technologies that will lead to it. "23:23
JayDuggerA few paragraphs in, Lofstrom says "Nuclear power frightens people." Later, in the same NBF interview he discusses space-based solar power. (Yeah, as if that won't scare the timid.)23:28
MokstarI like the roving death beam one23:35
JayDuggerYeah...power density matters.23:36
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--- Log closed Sat Oct 06 00:00:47 2012

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