| --- Log opened Thu Feb 14 00:00:00 2013 | ||
| nmz787 | kanzure: $1500 looks like he's using the same projector like you sent me http://sedgwick3d.com/ | 00:10 |
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| nmz787 | "By using this system we were able to do custom photolithography in a much shorter time that we could have using | 00:39 |
| nmz787 | masks. The time between the formulation of an idea to a | 00:39 |
| nmz787 | completed project can be less than 20 min." | 00:39 |
| nmz787 | kanzure: this is cool http://www.thingiverse.com/thing:29735 | 00:43 |
| nmz787 | i'm not sure what it means 'with reprap technologies' | 00:44 |
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| @kanzure | nmz787: have you looked at lemoncurry? | 01:01 |
| @kanzure | wtf happened to thingiverse's stylesheet. | 01:02 |
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| nmz787 | paperbot http://144.206.159.178/FT/CONF/16419087/16419119.pdf | 01:17 |
| nmz787 | kanzure: that shows drop storage | 01:17 |
| @kanzure | gotta have the colon | 01:17 |
| @kanzure | paperbot: http://144.206.159.178/FT/CONF/16419087/16419119.pdf | 01:17 |
| paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/37083933a3ea7dff998d78616918def6.pdf | 01:17 |
| nmz787 | kanzure: yeah i've looked at it, not in a week or so | 01:17 |
| nmz787 | when i was just scanning it for resin links | 01:18 |
| nmz787 | i found a UK company that has cheap resin, but shipping is crazy expensive | 01:18 |
| @kanzure | anyway, here's what i was reading on droplet storage: | 01:18 |
| @kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/Simple,%20robust%20storage%20of%20drops%20and%20fluids%20in%20a%20microfluidic%20device.pdf | 01:18 |
| nmz787 | something about their post office having an embargo for sending hazardous materials to the u.s. | 01:18 |
| nmz787 | kanzure: do you have the accompanying autocad file? | 01:22 |
| nmz787 | "An AutoCAD le of the actual device | 01:22 |
| nmz787 | is included in the ESI (S7.dwg)." | 01:22 |
| @kanzure | nope not on me. ESI sounds like "electronic supplementary information". | 01:30 |
| @kanzure | i wonder if the zotero translators are able to pick up links to supplementary pdfs | 01:34 |
| @kanzure | if not, that's a rather serious omission. | 01:35 |
| nmz787 | that would be nice | 01:35 |
| nmz787 | they're often not pdfs though | 01:35 |
| nmz787 | sometimes avi files, or in this case dwg | 01:35 |
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| chris_99 | has anyone used http://www.addgene.org/ | 04:56 |
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| strangewarp | http://www.nytimes.com/2013/02/15/sports/oscar-pistorius-shooting-south-africa.html?_r=2& - Oscar Pistorius, first person to quality for the Olympics while using prosthetics, and noted for his usefulness as a rhetorical device in transhumanist discussions, murders girlfriend | 07:15 |
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| ThomasEgi | sounds like the story around reiserfs | 07:42 |
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| delinquentme | effing langton labs website is down | 12:27 |
| delinquentme | =[ | 12:27 |
| delinquentme | poopy blogspot | 12:27 |
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| @kanzure | http://repo.steampowered.com/steam/pool/steam/s/steam/steam64_1.0.0.27_amd64.deb | 12:59 |
| ThomasEgi | yay. 64bit linux steam | 12:59 |
| nmz787 | is that really hplus related? | 13:01 |
| nmz787 | not that i care | 13:01 |
| chris_99 | am i right in thinking something like this plasmid http://www.addgene.org/22308/ would create GFP when inserted into yeast | 13:06 |
| nmz787 | yes | 13:06 |
| nmz787 | when galactose is added | 13:06 |
| chris_99 | aha ok, cheers | 13:07 |
| @kanzure | nmz787: no, it's not. lapse of judgement. | 13:08 |
| nmz787 | chris_99: http://en.wikipedia.org/wiki/Gal_operon | 13:10 |
| chris_99 | thanks | 13:11 |
| @kanzure | "reminiscent of the case of Gordon & Breach v. American Institute of Physics and American Physical Society which started with the publication of articles on the cost of physics journals by H.H. Barschall" | 13:11 |
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| @kanzure | http://www.insidehighered.com/news/2013/02/08/academic-press-sues-librarian-raising-issues-academic-freedom | 13:12 |
| chris_99 | also i've been looking at this http://labs.fhcrc.org/gottschling/Yeast%20Protocols/ytrans.html the bottom protocol, so is it more or less a case of charging a 25uF cap, then discharging via a 200 ohm resistor to the cuvette | 13:12 |
| @kanzure | what is Edwin Mellen Press anyway? | 13:13 |
| nmz787 | chris_99: electroporation has a few general methods | 13:13 |
| nmz787 | the type of decay, a cap is exponential, is important and can be played with to give different results | 13:14 |
| nmz787 | length of decay, voltage | 13:14 |
| nmz787 | i was unsuccessful at transforming ecoli with a grill lighter piezo sparker | 13:14 |
| chris_99 | mm, which is varied with the resistor i guess, and the initial charging voltage | 13:14 |
| chris_99 | aha | 13:14 |
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| nmz787 | but that was just based on the sparker putting out some average value i got from diybio | 13:15 |
| nmz787 | and then changing the distance between electrodes | 13:15 |
| nmz787 | so i must have been way off in some paramaeter | 13:15 |
| nmz787 | it looks like that protocol wants SS-DNA, but I'm not sure if they mean ssDNA (single-stranded) | 13:17 |
| chris_99 | oh i didn't notice that | 13:17 |
| nmz787 | oh it was the first protocol | 13:17 |
| nmz787 | with LiAc | 13:17 |
| chris_99 | hmm, i've got a PSU that can do KV hopefully, i'd need to get the necessary caps / resistors though. | 13:18 |
| nmz787 | " Give one pulse: V= 1.5kV, 25 F, 200 Ohms. Time should be ~4-5"" | 13:18 |
| nmz787 | 4-5 minutes??? | 13:18 |
| nmz787 | i wonder if you could charge it with a piezo sparker | 13:18 |
| nmz787 | they're around 10kv i think | 13:19 |
| nmz787 | if i recall correctly | 13:19 |
| archels | 1.5 kV over what electrode spacing? | 13:19 |
| nmz787 | i'm bad when it comes to capacitors and charge and discharge | 13:19 |
| nmz787 | 0.2 cm | 13:19 |
| chris_99 | 0.2cm cuvette | 13:19 |
| nmz787 | chris_99: that means you could use a 0.1cm cuvette with 750V | 13:20 |
| nmz787 | i'm not sure if you'd need to change the ohms though | 13:20 |
| chris_99 | do http://www.addgene.org/ ship to residential addresses? | 13:20 |
| nmz787 | prob not | 13:20 |
| nmz787 | from what i heard recently you need to be non-profit or academic | 13:21 |
| nmz787 | industrial users only have access to a subset of the dna | 13:21 |
| archels | you guys just need to build some Cockcroft-Walton voltage boosters | 13:21 |
| chris_99 | mm, i got some caps to do that | 13:21 |
| nmz787 | the professional electroporator i took apart used SCRs if that's worth anything | 13:21 |
| archels | chris_99: great, just add diodes =) | 13:22 |
| chris_99 | is there anywhere else that would send plasmids | 13:22 |
| @kanzure | sciquest is asking for me to make a website with a picture of my product | 13:24 |
| @kanzure | i don't have a product. quick, someone make up a product! | 13:24 |
| nmz787 | send openspectrometer | 13:25 |
| @kanzure | ah good idea | 13:25 |
| @kanzure | the deed has been done. | 13:26 |
| archels | actually, I think laser printers have DC high voltage modules | 13:28 |
| chris_99 | mm, i think your right | 13:30 |
| nmz787 | kanzure: what's sciquest? | 13:35 |
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| nmz787 | omg i have this jalokia pepper beef stick | 13:35 |
| nmz787 | its made me hiccup | 13:35 |
| nmz787 | ahh, it's a text file | 13:41 |
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| nmz787 | kanzure: they have asection on select agents | 13:43 |
| nmz787 | SE L E C T AGE NT S : | 13:44 |
| nmz787 | Definition: Some products are live cells of extremely hazardous pathogens such as Ebola virus, Marburg virus etc. | 13:44 |
| nmz787 | The CDC has set stringent regulations about all aspects o | 13:44 |
| nmz787 | products. | 13:44 |
| nmz787 | Usage: This Product Flag should be turned on for any product that is considered to be a select agent as per CDC | 13:44 |
| nmz787 | definition. | 13:44 |
| nmz787 | Example: Monkeypox virus, Brucella melitensis, Bovine spongiform e | 13:44 |
| nmz787 | Note: Additional details about CDC regulations about select agents can be | 13:44 |
| nmz787 | http://www.cdc.gov/od/sap/docs/salist.pdf | 13:44 |
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| @kanzure | sciquest is just some lab equipment leasing.. thing. | 13:54 |
| nmz787 | kanzure: do you have any papers on light controlled microfluidic valves? | 13:56 |
| nmz787 | I saw something yesterday that showed that | 13:56 |
| nmz787 | i was thinking it might be nice if the energy densities are practical with a DLP, though I doubt they are, I'm not sure at all | 13:57 |
| nmz787 | I'm figuring out energy density stuff now for resins | 13:57 |
| @kanzure | not really | 13:57 |
| @kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/Single-sided%20continuous%20optoelectrowetting%20(SCOEW)%20for%20droplet%20manipulation%20with%20light%20patterns%20-%20LCD%20-%202010.pdf | 13:57 |
| @kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/Light-driven%20droplet%20manipulation%20technologies%20for%20lab-on-a-chip%20applications.pdf | 13:58 |
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| nmz787 | kanzure: heard of 3dsystems.com? | 14:09 |
| @kanzure | yes. | 14:10 |
| @kanzure | dassault-owned, so it's a heavy tie-in to solidworks. | 14:10 |
| nmz787 | are there any $500-$750 range 3d printers available off-the-shelf? | 14:13 |
| @kanzure | reprap. | 14:14 |
| @kanzure | http://www.ebay.com/sch/i.html?_from=R40&_sacat=0&_nkw=prusa+reprap&_sop=3 | 14:14 |
| nmz787 | are there nicer looking chinese ripoffs? | 14:15 |
| @kanzure | why would you care about nicer looking :( | 14:16 |
| nmz787 | to get a better feel for how to redeisgn one | 14:16 |
| @kanzure | http://wiki.reprap.org/ | 14:17 |
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| @kanzure | also, there's 100s of people in #reprap that you should talk with | 14:17 |
| nmz787 | this looks much cleaner http://cubify.com/cube/ | 14:17 |
| nmz787 | than this http://www.ebay.com/itm/3D-Printer-reprap-prusa-mendel-complete-package-prototyping-UP-Plus-NEW-/111011737070?pt=LH_DefaultDomain_0&hash=item19d8d0adee | 14:17 |
| @kanzure | i guarantee that you will get better information from #reprap | 14:17 |
| nmz787 | ahh | 14:17 |
| @kanzure | seems they are up to 463 people today. | 14:18 |
| nmz787 | that wiki just says it works! | 14:18 |
| @kanzure | wow wtf | 14:19 |
| @kanzure | ah they moved it | 14:19 |
| @kanzure | http://reprap.org/wiki/Main_Page | 14:19 |
| @kanzure | nmz787: i also recommend /join #lemoncurry | 14:22 |
| @kanzure | http://www.adafruit.com/blog/2013/02/12/limor-fried-ladyada-joining-president-obama-in-a-fireside-hangout-this-thursday-at-450pm-est-on-google/ | 14:27 |
| @kanzure | limor fried and obama? sounds good to me. | 14:27 |
| streety | is anyone watching that? I've only just got in | 14:32 |
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| @kanzure | i think it's at 43min 30sec | 14:32 |
| @kanzure | this isn't what i was expecting. it's just a few questions she sent in. | 14:32 |
| @kanzure | she's just asking about software patents. | 14:33 |
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| streety | fair enough, I've just joined the live stream at 1hr5min | 14:35 |
| streety | the end was quite good, ". . . are we done?" | 14:44 |
| @kanzure | http://www.reddit.com/r/Scholar/comments/18gwnk/removing_watermarks_from_academic_papers/ | 14:45 |
| @kanzure | "Nota bene: removing a watermark will shatter your chances of calling paper-sharing "fair use" if challenged in court" | 14:46 |
| @kanzure | does he have any evidence? | 14:46 |
| ParahSailin | i dont think thats too wild of an assertion | 14:47 |
| @kanzure | not what i'm asking... case law. | 14:47 |
| ParahSailin | court precedent is publishers always win and prosecutors are overzealous | 14:47 |
| @kanzure | i haven't seen anything about an academic watermark ever being used in court. | 14:48 |
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| nmz787 | kanzure: adduser or useradd? | 15:01 |
| nmz787 | kanzure: which makes all the dirs and stuff? | 15:01 |
| @kanzure | adduser | 15:03 |
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| @kanzure | xkcd's discussion about steroids is horribly under-informed http://forums.xkcd.com/viewtopic.php?f=7&t=100281 | 17:06 |
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| bkero | Surprise? | 17:11 |
| @kanzure | yes, it was a pro-steroid comic but the responses are all negative. | 17:12 |
| streety | Is that so surprising? If you disagree with the comic would you not be more likely to comment? | 17:20 |
| @kanzure | yes, usually xkcd responses include links to wikipedia and shit, but even the pro-steroids users aren't posting those refutations. | 17:20 |
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| streety | A higher % of first-timers perhaps? | 17:23 |
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| @kanzure | paperbot: http://europepmc.org/abstract/MED/8969015/reload=0;jsessionid=PcANClGdQ0ZNoS0uvDpy.6 | 17:50 |
| paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/78289f60d27e97973ee4cf6b2ab0017b.txt | 17:50 |
| @kanzure | paperbot: http://www.sciencedirect.com/science/article/pii/S0306453002000252 | 17:51 |
| paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Neuroendocrine%20and%20behavioral%20effects%20of%20high-dose%20anabolic%20steroid%20administration%20in%20male%20normal%20volunteers.pdf | 17:51 |
| @kanzure | "Psychoneuroendocrinology" is an almost ridiculous journal title | 17:57 |
| yashgaroth | "scandinavian journal of experimental psychoneuroendocrinology" | 17:58 |
| strangewarp | the xkcd forums are full of people who love feeling smart without doing the legwork | 18:00 |
| @kanzure | "soviet journal of letters in experimental psychoneuroendocrinology, volume ш" | 18:00 |
| @kanzure | hm i wonder if there's a "soviet journal" still around somewhere | 18:00 |
| @kanzure | aha... Soviet Journal of Physical Oceanography. | 18:00 |
| @kanzure | Soviet Journal of Experimental | 18:00 |
| @kanzure | and Theoretical Physics | 18:00 |
| @kanzure | aww they dropped the "soviet" from the title http://www.springer.com/physics/particle+and+nuclear+physics/journal/11447 | 18:01 |
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| @kanzure | paperbot: http://www.springer.com/realtimeTopDownload/download/fe6c875d-00fe-460f-97c5-3ad5eb4e91f8/10.1007/s12024-010-9198-1.pdf | 18:17 |
| paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/c3763bacaf5e38a22fbfdcf01dd8e804.txt | 18:17 |
| @kanzure | CVE-2012-1089 "Directory traversal vulnerability in Apache Wicket 1.4.x before 1.4.20 and 1.5.x before 1.5.5 allows remote attackers to read arbitrary web-application files via a relative pathname in a URL for a Wicket resource that corresponds to a null package" | 18:18 |
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| SphericalMouse | Sup? | 19:08 |
| docl | howdy | 19:11 |
| SphericalMouse | any excitement? | 19:12 |
| JayDugger | Happy Valentine's Day! | 19:12 |
| docl | I'm working on a wiki for cryo stuff | 19:13 |
| archbox_ | JayDugger: you too | 19:13 |
| @kanzure | docl: would you consider just using diyhpluswiki.git? | 19:13 |
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| @kanzure | ParahSail1n: hey did you happen to read the backlog with SphericalMouse yesterday? | 19:14 |
| ParahSail1n | i missed it | 19:14 |
| SphericalMouse | he's a terrible typist | 19:14 |
| docl | kanzure: any particular reason I should? | 19:15 |
| @kanzure | docl: because putting transhumanist information in the same place increases the likelihood that contributors will maintain the information. | 19:16 |
| ParahSail1n | what was the backlog about, im on a different machine from usual | 19:17 |
| docl | my theory is that multiple different sites/communities is good for diversity or some such. also my ego is more flattered at the thought of starting my own thing. | 19:18 |
| @kanzure | ParahSail1n: SphericalMouse is interested in lab automation, and also has some experience with microfluidics. | 19:19 |
| @kanzure | docl: what about just forking the repo? :) | 19:20 |
| docl | hmm. maybe. I'm using foswiki at the moment. | 19:20 |
| docl | what's diyhpluswiki.git in? | 19:20 |
| @kanzure | have you ever used git? | 19:20 |
| @kanzure | well, it's just a collection of files that can be rendered any way you please. i use ikiwiki. | 19:20 |
| @kanzure | http://diyhpl.us/wiki is the front-end | 19:20 |
| @kanzure | https://github.com/kanzure/diyhpluswiki is the git repo | 19:21 |
| ParahSail1n | SphericalMouse, thats cool | 19:21 |
| docl | I've played around a bit with github | 19:21 |
| SphericalMouse | indeed, what is your interest in the area? | 19:21 |
| @kanzure | docl: the downside is that the default ikiwiki stylesheets don't look like mediawiki's monobook.css layout. i guess that might be one reason you might not use this. | 19:21 |
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| ParahSail1n | i think it would be cool to have a cell sorter | 19:22 |
| SphericalMouse | what kind of cells? | 19:23 |
| ParahSail1n | mammal i suppose | 19:23 |
| ParahSail1n | facs is super expensive | 19:23 |
| SphericalMouse | what do you want to sort based upon? | 19:24 |
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| ParahSail1n | if i had one, im sure i could think of a few things to do | 19:24 |
| SphericalMouse | well, there are different ways to sort things | 19:25 |
| docl | for now, the wiki is at http://bettercryonics.com/BetterCryonics | 19:25 |
| SphericalMouse | bead/surface binding, dielectrophoresis, electrophoresis, size based, density based | 19:27 |
| SphericalMouse | blah blah | 19:27 |
| ParahSail1n | most of the sorting id want to do would probably be based on fluor markers | 19:27 |
| SphericalMouse | urff | 19:28 |
| ParahSail1n | it would be nice to just have a flow cytometer to take measurements of population even | 19:29 |
| SphericalMouse | I suppose you could flow things at low density down a y channel and have some force push things from one side to another | 19:29 |
| SphericalMouse | oh, thats pretty simple, as things go | 19:29 |
| SphericalMouse | inverted flourescent microscope with camera | 19:30 |
| ParahSail1n | i did some experiments on facscanto, but it would be pretty cool to be able to afford one | 19:30 |
| SphericalMouse | what do you have? | 19:31 |
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| ParahSail1n | well i was a grad student for a while doing wet lab, now i'm kinda out of that area | 19:33 |
| SphericalMouse | what equipment or facilities do you have access to? | 19:34 |
| ParahSail1n | a pirate version of the agilent bioanalyzer would be cool too, for qc of ngs library prep | 19:34 |
| ParahSail1n | nothing at the moment | 19:34 |
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| @kanzure | docl: do you mind if i import content into diyhpluswiki.git? | 19:35 |
| docl | I don't mind | 19:35 |
| SphericalMouse | how much are you willing to spend? | 19:36 |
| ParahSail1n | it depends on the upside? | 19:37 |
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| SphericalMouse | say you could take pictures of cells, extract the fluorescence level, and look at size and shape? would that be what you want? | 19:38 |
| nmz787 | howdy | 19:38 |
| ParahSail1n | well, i could probably find a good deal on that sort of stuff on slapsale | 19:39 |
| SphericalMouse | on what? | 19:40 |
| ParahSail1n | fluorescence microscopy | 19:41 |
| nmz787 | hey a site that isn't ebay! | 19:41 |
| nmz787 | any ideas on how to hook up a fridge peltier to act as a still chiller? | 19:41 |
| SphericalMouse | everclear is pretty cheap | 19:42 |
| SphericalMouse | well, what would your overall budget be? | 19:44 |
| nmz787 | this looks pretty nice http://www.slapsale.com/item_desc.aspx?itemid=63601 | 19:45 |
| nmz787 | hm, maybe not | 19:46 |
| nmz787 | sorry | 19:46 |
| SphericalMouse | unfortunately, that is not a brand of fluorescent microscope i am familiar with | 19:46 |
| nmz787 | hey there are a few good looking deals, ParahSail1n is this all local pickup? | 19:47 |
| ParahSail1n | nmz787, yes | 19:47 |
| nmz787 | this scope is pretty nice if the price stays low http://www.slapsale.com/item_desc.aspx?itemid=63705 | 19:48 |
| SphericalMouse | usually you want an inverted microscope | 19:49 |
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| SphericalMouse | especially for micofluidics | 19:50 |
| nmz787 | why? | 19:50 |
| SphericalMouse | various accesibility reasons. often there will be plumbing, which is easier to deal with if ti comes out the top, but then it bangs into the objectives of an upright scope | 19:51 |
| nmz787 | huh, why don't people just make manifolds that have sideports? | 19:51 |
| nmz787 | like a CPU socket | 19:52 |
| SphericalMouse | ~lolzz~ | 19:52 |
| nmz787 | where an arm comes and closes over the top | 19:52 |
| nmz787 | compresses down | 19:52 |
| SphericalMouse | oftne pdms microfluidics will have a thin and thick side, and a scope wont be able to image through the thick side, andyou want the thin side down | 19:53 |
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| SphericalMouse | every fluid connection i have ever seen comes in through the top or bottom, never the side | 19:54 |
| nmz787 | weird | 19:54 |
| SphericalMouse | consequence of manufacturing | 19:54 |
| SphericalMouse | and geometry | 19:55 |
| JayDugger | Would you have ullage problems without gravity draining the channel? | 19:55 |
| ParahSail1n | yeah ive only seen vias at top and bottom | 19:55 |
| ParahSail1n | gravity can't make enough pressure to make flow through microchannel | 19:55 |
| JayDugger | Fair enough. | 19:56 |
| SphericalMouse | you can have gravity driven flow issues, typially it is low compared to other forces, but you can see it if you're not careful | 19:56 |
| SphericalMouse | just blance your inlet ant outlet heights | 19:56 |
| SphericalMouse | goddamn wine | 19:56 |
| SphericalMouse | I would use about 5-10 psi do drive fluid through microchannels, internet says 1 psi is a delta of 27 inches of water | 19:58 |
| SphericalMouse | i have seen people use sliding vials on rails as a pressure source though | 20:05 |
| nmz787 | i think i saw something like that not too long ago | 20:07 |
| SphericalMouse | i alsways used a gas line with a tunable regulator, but the rail will work as well, probably more accurate, but getting 5 psi would require a 10 footish rail | 20:08 |
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| @kanzure | Juul: do you have the storage space configured yet? | 21:51 |
| @kanzure | for libgen? | 21:51 |
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| @kanzure | it looks like mendeley might be interested in donating rule 34 relevant artwork for launching springerkink.com | 22:07 |
| @kanzure | yashgaroth: it will be glorious | 22:08 |
| yashgaroth | not even sure what to say to that | 22:09 |
| Juul | kanzure, storage space? i have a 4 tb harddrive but only about 15% downloaded | 22:10 |
| @kanzure | well i'm just tired of not having access to springerlink.com | 22:10 |
| Juul | it's been taking longer than it should due to some suspicious network issues | 22:10 |
| Juul | (possibly trolls but could be stupidity) | 22:10 |
| Juul | basically someone came in and hard reset two routers | 22:11 |
| yashgaroth | do you share the routers with people? | 22:13 |
| yashgaroth | if you overloaded the router and someone else using it got pissed off and reset it | 22:13 |
| yashgaroth | then again I have no idea what your setup is | 22:15 |
| Juul | yashgaroth, i rate limited everything. but yeah it was in a shared space. we've been getting trolled though, so it may be trolls at work | 22:27 |
| Juul | they also fucked the network by doing that since it created a rogue dhcp server | 22:28 |
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| --- Log closed Fri Feb 15 00:00:01 2013 | ||
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