| --- Log opened Wed May 01 00:00:13 2013 | ||
| kanzure | ryankarason: also, you can pick them up pretty cheap on ebay and alibaba | 00:15 |
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| archels | http://blaauw.eecs.umich.edu/project.php?id=66&sid=d4c39954d090d21e5974cfcfa0324ded | 02:25 |
| archels | .title | 02:25 |
| yoleaux | archels: Sorry, that doesn't appear to be an HTML page. | 02:25 |
| archels | no botsnacks for you, yoleaux | 02:26 |
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| kanzure | hfempire seems to let you buy DDOS attacks. not sure.. it doesn't ask you for the target. | 03:13 |
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| ryankarason | kanzure: what exactly would i be looking for, a "dna sequencing machine" ? and are there any diy tutorials for building one ? | 07:40 |
| kanzure | yes, you would type in "dna sequencer" | 07:55 |
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| ryankarason | kanzure: what would you say is "pretty cheap" | 08:21 |
| ryankarason | and also, do you have a dna sequencer? | 08:21 |
| kanzure | pretty cheap is anything under $10,000 in this case | 08:28 |
| kanzure | no, i don't presently have a dna sequencer | 08:28 |
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| ParahSai1in | http://bostonherald.com/business/technology/technology_news/2013/04/genetic_research_toolmaker_raindance_technologies_raises | 10:11 |
| kanzure | "and the recently launched RainDrop digital PCR system for ultra-sensitive multiplexed quantitative detection of circulating nucleic acids." | 10:13 |
| kanzure | what does that even mean.. circulating. so it's a thermocycler that you put into your bloodstream? | 10:13 |
| kanzure | or "circulating within the confines of a micropipette tube" | 10:13 |
| kanzure | oh god i don't even remember what to call them. microcentrifuge tubes? micropipette tubes? | 10:14 |
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| archels | Google Glass has competition already - http://tele-pathy.org/ | 12:06 |
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| rk[woodsing] | http://openpcr.org/ | 13:24 |
| rk[woodsing] | $600, would more be what i would define as "cheap" | 13:25 |
| kanzure | yes we're aware of openpcr. it's really expensive, but cheaper than the other commercial stuff. | 13:25 |
| kanzure | there's also a $75 thermocycler on github called wiremound or something | 13:26 |
| kanzure | and then there's russell's thing, uh, lightbulb pcr something | 13:26 |
| chris_99 | why is openpcr so expensive | 13:26 |
| rk[woodsing] | hmm, well why would you say $10k is cheap | 13:26 |
| chris_99 | what's the main cost in it | 13:26 |
| chris_99 | cheap for a sequencer he said | 13:27 |
| rk[woodsing] | is openpcr a sequencer? | 13:27 |
| chris_99 | no | 13:27 |
| rk[woodsing] | what is the difference? | 13:27 |
| chris_99 | it just replicates DNA | 13:27 |
| kanzure | wtf chris_99 | 13:27 |
| chris_99 | if that's the right term | 13:27 |
| kanzure | no it doesn't replicate dna | 13:27 |
| chris_99 | sorry what's the right term | 13:28 |
| kanzure | openpcr is a thermocycler. it changes the temperature of small liquid samples. | 13:28 |
| chris_99 | *amplifies | 13:28 |
| kanzure | what? | 13:28 |
| rk[woodsing] | so what are the parts/steps for dna sequencing. dna extraction, dna amplification, and dna detection. so some extracting medium, a thermocycler, and what is technology is used for detection ? | 13:36 |
| rk[woodsing] | those are the three steps i understand from my recent readings... | 13:36 |
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| kanzure | i would start by reading up about sanger sequencing, pyrosequencing, next gen sequencing, etc. | 13:38 |
| ryankarason | do you have any good links for such topics, or should i just start the random searching? | 13:40 |
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| kanzure | wikipedia. | 13:44 |
| kanzure | http://en.wikipedia.org/wiki/Sanger_sequencing | 13:44 |
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| kanzure | "in fact, it's illegal to sell water "contaminated" with magnesium" | 14:09 |
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| superkuh | paperbot: http://www.jneurosci.org/content/33/15/6460.full.pdf+html | 17:11 |
| paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Increases%20in%20cAMP%2C%20MAPK%20Activity%2C%20and%20CREB%20Phosphorylation%20during%20REM%20Sleep%3A%20Implications%20for%20REM%20Sleep%20and%20Memory%20Consolidation.pdf | 17:11 |
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| kanzure | jmil: hey how goes rice? | 17:42 |
| superkuh | paperbot: http://www.sciencemag.org/content/340/6131/449.long | 17:47 |
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| jmil | kanzure: sweeeeeet | 19:55 |
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| --- Log closed Thu May 02 00:00:02 2013 | ||
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