| --- Log opened Thu May 02 00:00:02 2013 | ||
| --- Day changed Thu May 02 2013 | ||
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| jeffz | hi, anyone with a US university institutional login have access to this? http://babel.hathitrust.org/cgi/pt?id=mdp.39015039846566 | 00:04 |
|---|---|---|
| jeffz | trying to get the full pdf | 00:04 |
| kanzure | bleh you don't need that. | 00:07 |
| jeffz | I do if i want to read it without the pages constantly reloading when I scroll through it. | 00:08 |
| jeffz | just thought someone might be able to do me a favor | 00:09 |
| kanzure | i mean, perceptrons aren't exactly that great | 00:09 |
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| kanzure | google glass winking api https://news.ycombinator.com/item?id=5642924 | 00:35 |
| kanzure | http://glass-apps.org/winky-google-glass-app | 00:35 |
| kanzure | https://plus.google.com/116031914637788986927/posts/Hp9n6oXpFfH | 00:37 |
| kanzure | https://github.com/kaze0/winky | 00:37 |
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| chido | ~. | 01:20 |
| chido | ~> | 01:20 |
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| archels | "Google Glass is the future – and the future has awful battery life" | 02:57 |
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| Urchin | hi people | 06:50 |
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| delinquentme | jmil, I bought a magazine. | 11:34 |
| delinquentme | you were in it. gj | 11:34 |
| kanzure | https://github.com/kanzure/pdfparanoia/pull/25 "I've written a script tests/diff/comparediff which automates downloading PDFs from two different sources, and verifying that pdfparanoia makes the files byte-for-byte identical." | 11:37 |
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| DonnchaC | kanzure: Nice work, I was gonna implement something similar in the next few weeks | 11:44 |
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| jmil | delinquentme: wha? | 11:46 |
| delinquentme | jmil, it was a science mag | 11:48 |
| delinquentme | i guess whats crazy though is how long it takes that information to get into magazines | 11:48 |
| jmil | delinquentme: wow cool. know which one? | 11:48 |
| delinquentme | are you still working on that? | 11:48 |
| jmil | still working on sugar printing for organ printing? YES. it is a 30 yr project and I'm all in on it | 11:48 |
| delinquentme | It seems that decellularization seems a quicker path to that end :D | 11:49 |
| jmil | starting my own research lab at Rice University as assistant professor of bioengineering in a couple months | 11:49 |
| delinquentme | i guess why not perfusion decellularization though | 11:49 |
| jmil | delinquentme: decellularization is interesting, but it has no control and no reproducibility (every organ is different). they don't have a reproducible way to re-seed cells into the tissue. | 11:49 |
| delinquentme | other than " I have money for 3d printing sugar structures " | 11:49 |
| delinquentme | what? | 11:49 |
| delinquentme | are you sure? | 11:50 |
| delinquentme | from what I've read its simply an issue of negative pressure in the right areas... | 11:50 |
| jmil | i am watching the space carefully. it is certainly very interesting. but i've talked to the experts in person and they admit total lack of control or ways to assay the tissue, which will HAVE to be done before it gets into a human patient | 11:50 |
| kanzure | DonnchaC: i didn't write it | 11:51 |
| jmil | the risk of blood clots is insanely high. in fact, that's why they always fail | 11:51 |
| jmil | so far | 11:51 |
| kanzure | jmil: let me know when you are going to be stocking your lab. i'd be happy to suggest toys and goodies. | 11:51 |
| jmil | kanzure: serious? that would be aweosme | 11:51 |
| delinquentme | assay the tissue for what? | 11:52 |
| jmil | delinquentme: yes well that hasn't even been defined yet | 11:52 |
| jmil | i am developing a research program to actually define what we need to assay | 11:52 |
| DonnchaC | kanzure: woops, just assumed you did from the git comments I read on my phone earlier | 11:52 |
| delinquentme | I'm missing something here. You need to qualify what things we need to look at pre-implantation? | 11:52 |
| jmil | delinquentme: for example, of the cells injected, where do they go in the tissue hierarchy? how many stay alive? | 11:52 |
| kanzure | DonnchaC: no, that was a contribution from someone else | 11:52 |
| jmil | delinquentme: you can't do imaging in a block of tissue that big. so if you have a huge life-threatening blood clot in the center of the structure you will never know it until it kills the patient | 11:53 |
| DonnchaC | Yeah I see that now, useful none the less | 11:53 |
| delinquentme | I mean you know they've done nothing more than apply negative pressure, circulate a fluid of cells and then apply given physical stimulus ... and subsequently gotten lungs running in a rat right? | 11:53 |
| kanzure | jmil, what did you think of that recent hydrogel/imaging paper? | 11:53 |
| jmil | delinquentme: yes, but that was only for a few hours before it clotted out | 11:54 |
| jmil | kanzure: which paper? | 11:54 |
| kanzure | the CLARITY paper | 11:54 |
| jmil | delinquentme: i'm very interested in decellularization, but it lacks a certain level of control which i desire for quantitative cell biology studies | 11:55 |
| kanzure | this one, http://diyhpl.us/~bryan/papers2/paperbot/Structural%20and%20molecular%20interrogation%20of%20intact%20biological%20systems.pdf | 11:55 |
| kanzure | oh oops | 11:55 |
| kanzure | i mean http://diyhpl.us/~bryan/papers2/paperbot/7b0c273e70ca41c9d5e28f4efef62ada.pdf | 11:55 |
| kanzure | it does things like: | 11:55 |
| kanzure | http://www.nature.com/nature/journal/vaop/ncurrent/fig_tab/nature12107_SV14.html "Hippocampus region showing networks of YFP-expressing neurons (green), distribution of PV-positive neurons (red) and astrocytes (blue)." | 11:55 |
| jmil | kanzure: ya that is cool tech. i have a collaborator who knows how to do Scale. She mentioned CLARITY too | 11:56 |
| jmil | in Houston | 11:56 |
| jmil | at Baylor | 11:56 |
| kanzure | "Second, whereas passive diffusion of detergent micelles would take many months to completely extract lipids from the adult mouse brain, we developed an active-transport organ-electrophoresis approach, which we term electrophoretic tissue clearing (ETC), that capitalizes on the highly charged nature of ionic micelles." | 11:56 |
| kanzure | so anyway, organ electrophoresis | 11:56 |
| jmil | kanzure: yes it appears to work well, and i'm very familiar with acrylate monomers | 11:57 |
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| delinquentme | http://www.youtube.com/watch?v=DiyWXQyAJ44 | 13:09 |
| delinquentme | cat vs. crocodile | 13:09 |
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| jrayhawk | alligator | 13:12 |
| jrayhawk | alligators are relatively timid | 13:17 |
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| superkuh | paperbot: http://www.sciencemag.org/content/339/6118/452.long | 13:56 |
| paperbot | error: HTTP 300 http://diyhpl.us/~bryan/papers2/paperbot/Actin%2C%20Spectrin%2C%20and%20Associated%20Proteins%20Form%20a%20Periodic%20Cytoskeletal%20Structure%20in%20Axons.pdf | 13:56 |
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| ParahSai1in | michael j fox foundation is trying to insert human lrrk2 bac into a mouse genome | 14:27 |
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| kanzure | have i mentioned how much i hate javascript | 14:41 |
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| ThomasEgi | kanzure, now you did. | 14:43 |
| ParahSai1in | lol i've been playing with javascript to make an internal browser app | 14:50 |
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| ParahSai1in | i might have to rewrite in clojurescript or something | 14:50 |
| ParahSai1in | shits all just a mess | 14:51 |
| ThomasEgi | i only used javascript to build very simple media-player applications for a company. for that kind of stuff you can get away with 10 lines of javascript. compared with a lot of code in other languages. | 14:52 |
| ThomasEgi | guess for everything else javascript is pretty much a pita. | 14:52 |
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| abetusk | Did you guys see this: http://www.youtube.com/watch?feature=player_embedded&v=oRWeD2VFh7w | 15:45 |
| kanzure | .title | 15:45 |
| yoleaux | DNA testing chip delivers results in one hour, paves way for personalized drug treatments #DigInfo - YouTube | 15:45 |
| kanzure | yes, the news articles claimed it was sequencing, but it's pcr | 15:45 |
| abetusk | How close are we to doing this on a DIY level? | 15:46 |
| kanzure | pcr? | 15:46 |
| kanzure | uh, we've been doing pcr for a long time now.. | 15:46 |
| abetusk | No, miniaturized | 15:46 |
| kanzure | you can pcr a droplet of water with a laser if you wanted, is that small enough for you | 15:47 |
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| superkuh | paperbot: http://stm.sciencemag.org/content/4/140/140ra88.full.pdf | 16:14 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/82ee57c6013289b2b2e749a29c27fce1.txt | 16:14 |
| superkuh | paperbot: http://stm.sciencemag.org/content/4/140/140ra88.full | 16:15 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/c0091b11fec76a81da1ee0eb65cc9b2c.txt | 16:15 |
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| nmz787_ | ParahSai1in: TCD1304AP is the CCD I'm working with, it's the same one as the nanodrop my school had | 21:21 |
| nmz787_ | ParahSai1in: do you layout PCBs at all? ever use kicad? | 21:21 |
| kanzure | CERN is trying to fundraise a few euros to hire someone to work on kicad full-time | 21:22 |
| nmz787_ | yeah but not to help with projects, that's to make the software better | 21:23 |
| nmz787_ | but yes that's good news | 21:23 |
| nmz787_ | http://blog.al.com/wire/2013/03/ancient_underwater_forest_off.html | 21:23 |
| nmz787_ | pretty cool | 21:23 |
| kanzure | i think juri_ uses gEDA so maybe bug her about layout things | 21:23 |
| kanzure | juri_: are you also a layout wizard? i think you mentioned something the other day to this effect. | 21:24 |
| nmz787_ | geez i just assume everyone on here is a dude | 21:29 |
| nmz787_ | guess that's the faceless old internet | 21:29 |
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| ParahSail1n | nmz787_, no, i dont have much electronics experience | 22:22 |
| ParahSail1n | nmz787_, what do you have so far? | 22:24 |
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| --- Log closed Fri May 03 00:00:15 2013 | ||
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