| --- Log opened Thu Jan 23 00:00:42 2014 | ||
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| kanzure | bleep bloop | 05:45 |
|---|---|---|
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| JayDugger | bloop bleep (at more than 2 kb/s) | 06:53 |
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| kanzure | yep.. | 06:56 |
| pete4242 | How's the roadmap looking? Are we making progress? | 07:06 |
| kanzure | i'd say yes, why are you asking and who are you and why are you saying "we" | 07:09 |
| FourFire | pete4242, some things are happening, others aren't as fast ad we'd like | 07:09 |
| FourFire | but yeah, we're getting there | 07:10 |
| kanzure | fuck you FourFire you've never even seen the roadmap how would you know | 07:10 |
| kanzure | none of this makes sense | 07:10 |
| FourFire | kanzure, please don't swear at me withou reason | 07:10 |
| kanzure | it is good reason. i am highly confident that you haven't. | 07:10 |
| FourFire | PM | 07:10 |
| kanzure | i don't see a pm | 07:10 |
| pete4242 | I'm nobody (important). I mean 'we' as humanity.. | 07:11 |
| kanzure | oh, screw humanity | 07:11 |
| kanzure | you shouldn't care about whether or not "humanity" is making progress | 07:11 |
| pete4242 | You don't mind that people are suffering? | 07:11 |
| kanzure | what does that have to do wth anything? | 07:11 |
| kanzure | *with | 07:11 |
| pete4242 | It upsets me. | 07:11 |
| pete4242 | I think we have a moral duty to help people. | 07:12 |
| FourFire | pete4242, currently life sucks for a lot of people | 07:12 |
| pete4242 | no shit | 07:12 |
| FourFire | for a very big number of people it sucks so badly that they can't even sustain it | 07:12 |
| pete4242 | What should I care about, Kanz? | 07:12 |
| kanzure | well you're in a transhumanism channel | 07:12 |
| pete4242 | indeed | 07:12 |
| pete4242 | in the transhuman groove | 07:13 |
| kanzure | so i would say personal individual enhancement | 07:13 |
| kanzure | http://diyhpl.us/wiki/declaration is a fairly accurate portrayal of the shared values in this channel | 07:13 |
| pete4242 | I'm just one human. Why not try to enhance everyone? | 07:13 |
| pete4242 | then we can be transhumanS not just a transhuman | 07:14 |
| kanzure | uh.... | 07:14 |
| pete4242 | I wonder if that document has changed since I last looked at it. | 07:14 |
| kanzure | "why not try to enhance everyone?" do you include all lifeforms? | 07:14 |
| kanzure | are you an omega pointist or something | 07:14 |
| kanzure | like, do you believe that all lifeforms everywhere should be changed to have intelligence and then uploaded | 07:15 |
| kanzure | there's a strain of transhumanists that believe this, and i forget what they call themselves | 07:15 |
| kanzure | but i think you are one of them | 07:15 |
| kanzure | based on the available evidence you have presented me | 07:15 |
| pete4242 | You told me not to speculate, once | 07:15 |
| pete4242 | It's something to do with entropy, blah blah | 07:16 |
| pete4242 | I won't bore you with philosophy | 07:16 |
| kanzure | i'm not speculating, you just said "why not try to enhance everyone?" and it seems to be a conviction you were positing earlier | 07:16 |
| pete4242 | I don't know. I just like being happy, and I'm more happy when the whole universe is more happy. | 07:18 |
| gradstudentbot | Don't even ask how my research is going. | 07:18 |
| kanzure | are you david pearce? | 07:18 |
| pete4242 | hehe | 07:18 |
| kanzure | ? | 07:18 |
| pete4242 | No. I enjoy eating animals. | 07:18 |
| pete4242 | I'll eat anything dumber than me. | 07:18 |
| kanzure | but you see mto be aware of him | 07:18 |
| kanzure | *seem to | 07:19 |
| pete4242 | He's a friend. | 07:19 |
| pete4242 | I asked him to be the leader of my new cult. | 07:19 |
| kanzure | so are you asking me to show arguments for why i am more interested in personal human enhancement than global humanity enhancement (perhaps forced etc)? | 07:19 |
| kanzure | your.. new cult. | 07:19 |
| pete4242 | that's what I said. | 07:20 |
| pete4242 | wanna join? We'll have a cool bio lab. In Portugal. | 07:20 |
| kanzure | tiago rodrigues or vitorino ramos | 07:21 |
| kanzure | can't decide which one to guess | 07:21 |
| pete4242 | Are they people or places? | 07:21 |
| pete4242 | My name is Peter. | 07:22 |
| kanzure | i thought they would be people in the lab already but i guess not | 07:22 |
| kanzure | such slackers | 07:22 |
| pete4242 | There is no lab. yet. The people will come from all over the world. | 07:22 |
| kanzure | well they will probably show up eventually | 07:22 |
| pete4242 | Should we welcome them? | 07:22 |
| kanzure | then why would you say portugal | 07:22 |
| kanzure | i would appreciate it if you would say what you mean instead of bullshitting me | 07:23 |
| FourFire | "<pete4242> I'll eat anything dumber than me." yay, do you eat retards? | 07:23 |
| pete4242 | Because that's where we plan to build the lab. | 07:23 |
| kanzure | FourFire: cannibalism is a thing that happens you know.. | 07:23 |
| pete4242 | Only the delicious ones. | 07:23 |
| FourFire | kanzure, duh | 07:23 |
| pete4242 | I do try to say what I mean. Any help is appreciated. | 07:24 |
| kanzure | help about what | 07:24 |
| FourFire | so pete4242 what causes life as or more intelligent than yourself to have a right of happiness? | 07:24 |
| kanzure | ugh this is going to be a philosophical dick flinging contest right here | 07:25 |
| pete4242 | There are no rights. Just things I want and things I don't want. | 07:25 |
| kanzure | i'd rather you not get into an argument about hedonistic imperatives | 07:25 |
| pete4242 | I already told you I won't bore you with philosophy! | 07:25 |
| -!- mode/##hplusroadmap [+o kanzure] by ChanServ | 07:25 | |
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| FourFire | "dick flinging" gives an interesting mental picture | 07:26 |
| FourFire | it's a .gif | 07:26 |
| pete4242 | I don't want to know. | 07:26 |
| -!- mode/##hplusroadmap [+oooo fenn heath ParahSailin ybit] by kanzure | 07:26 | |
| pete4242 | I hate meat. | 07:26 |
| pete4242 | unless I'm eating it. | 07:26 |
| FourFire | cold, raw chicken breasts rubbing against your palms give you the willies? | 07:27 |
| pete4242 | Nah. Dead meat is fine. | 07:27 |
| pete4242 | I hate thinking meat. | 07:27 |
| FourFire | can we more closely define this "hate" ? because hate can mean anything from vaguely annoyed at to "will not stop until I rid the earth of this scourge upon existence" | 07:28 |
| pete4242 | heh. Kanz will get mad. So no. | 07:28 |
| -!- mode/##hplusroadmap [-o kanzure] by kanzure | 07:29 | |
| kanzure | can't we all just write software and build hardware | 07:29 |
| pete4242 | we can. | 07:29 |
| kanzure | instead of planning to grey gooify the world | 07:29 |
| kanzure | that will come naturally | 07:29 |
| FourFire | kanzure, I'd just like to state that I agree with parts of but not all of the declaration | 07:30 |
| pete4242 | edible hardware is good. No computation to do? well it makes a tasty snack! | 07:30 |
| FourFire | yeah, I'll go on with furthering life extension technology | 07:31 |
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| kanzure | i don't know what you mean | 08:20 |
| kanzure | please be more specific | 08:20 |
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| delinquentme | hue hue hue | 10:44 |
| delinquentme | ZOMGz | 10:44 |
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| Burninate | paperbot: http://rsta.royalsocietypublishing.org/content/371/1992/20120269.full.pdf+html | 12:35 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1098%2Frsta.2012.0269 | 12:35 |
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| delinquentme | paperbot, http://www.nature.com/nrd/journal/v5/n3/full/nrd1985.html | 15:36 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2Fnrd1985 | 15:36 |
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| delinquentme | 10 times the energy density of the lithium-ion batteries in your mobile devices. | 16:17 |
| delinquentme | sugar-powered-biobattery paperbot http://www.nature.com/ncomms/2014/140121/ncomms4026/full/ncomms4026.html | 16:17 |
| delinquentme | paperbot http://www.nature.com/ncomms/2014/140121/ncomms4026/full/ncomms4026.html | 16:19 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2Fncomms4026 | 16:19 |
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| drewbug | Anyone get their DNA sequenced? | 16:21 |
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| superkuh | Just 23andMe. | 16:56 |
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| jrayhawk | is there a sequencing service that does anything more than four codon types | 17:40 |
| jrayhawk | for DNA, that is | 17:40 |
| yash | four codon types? | 17:44 |
| -!- yash is now known as yashgaroth | 17:44 | |
| yashgaroth | like start, stop, coding, and mystery option? | 17:45 |
| jrayhawk | 5-methylcytosine, 5-hydroxymethylcytosine, etc. | 17:46 |
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| yashgaroth | probably not commercially for consumers, because it's super expensive compared to normal sequencing | 17:47 |
| yashgaroth | also, not very useful since there's a lot of variation between cells, and it's not really interesting outside of hard-to-sample tissues | 17:54 |
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| @ParahSailin | thats bases, not codons | 18:28 |
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| justanotheruser | gradstudentbot: | 18:34 |
| gradstudentbot | Paper submitted. | 18:34 |
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| @heath | did someone ever save this http://web.ornl.gov/sci/besd/bsd/nsd/education/afm_high.shtml | 19:58 |
| @heath | archive.org didn't | 19:58 |
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| drewbug | heath: http://web.archive.org/web/20100325192206/http://www.ornl.gov/sci/besd/bsd/nsd/education/afm_high.shtml | 20:12 |
| @heath | http://www.gizmag.com/lego-arduino-nanometer-resolution-atomic-force-microscope-lego2nano/29238/ http://lego2nano.openwisdomlab.net/ http://coidea.cc/project/2a954a49066468ff/solution/a27f0152587e08c6 http://lego2nano.openwisdomlab.net/team2.html http://lego2nano.openwisdomlab.net/img/450px-2+4_darklaser.jpg | 20:26 |
| @heath | cool, thanks drewbug | 20:26 |
| kanzure | hah, | 20:29 |
| kanzure | "The card fees gasoline retailers pay are staggering. They are on average the second-highest operating cost for gas retailers (higher than rent on their stores). The fees were more than $11 billion last year – 87% higher than the entire industry’s profits. That makes six years in a row that the industry paid more in card fees than it made in profits. Those fees add an average of 7 cents per gallon to the price of gasoline sold in the ... | 20:29 |
| kanzure | ... United States and the fees have been exploding. Between 2004 and 2011 while the price of gasoline went up 80%, card fees increased 180%" | 20:29 |
| kanzure | http://www.forbes.com/sites/beltway/2012/05/09/gas-station-operators-are-crying-over-credit-card-fees/ | 20:29 |
| kanzure | and the typical: "Ask any business owner who accepts credit cards about swipe fees and you'll get an earful. Every time a customer pays with a card, up to 4 percent of the sale goes back to the banks and credit card companies. Considering that the average profit margin for most retailers is 1-2 percent, these fees drive up prices and make it harder for small businesses to expand, hire more workers or even stay in business." | 20:30 |
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| Burninate | in China I was laughed at for trying to use my Visa-network check card and spent five minutes trying to explain why there was no pin number | 21:03 |
| kanzure | go on | 21:04 |
| Burninate | In the US, anything that goes over a credit network is loss-limited in the event of theft | 21:04 |
| Burninate | whereas if you have a debit card magstripe and pin number you can empty the entire account | 21:05 |
| Burninate | apparently basic credit card transactions are usually pin-protected in China | 21:05 |
| gradstudentbot | I think my PI hates me. | 21:05 |
| Burninate | I have a check card that can go through either network, credit card or debit card | 21:06 |
| drewbug | But you didn't want to do a debit transaction. | 21:06 |
| Burninate | right, because vulnerable | 21:06 |
| Burninate | and they were at a loss for why I would choose the option that involved no pin number at all, or why sucha n option existed | 21:06 |
| drewbug | Heh. | 21:06 |
| drewbug | Did it eventually work? | 21:06 |
| Burninate | yes | 21:06 |
| Burninate | the card itself never failed me, but the banks do have some pretty restrictive policies on overseas cash advance | 21:07 |
| kanzure | yeah, my bank has this weird text box where it asks you to type in travel plan details | 21:07 |
| Burninate | try to get cash out of an ATM and you might find yourself limited to 500 yuan at a time | 21:07 |
| kanzure | no specific format | 21:07 |
| kanzure | just type in the places, some words about your trip | 21:08 |
| kanzure | and magically none of the transactions will be flagged | 21:08 |
| kanzure | seems suspicious | 21:08 |
| kanzure | i don't think they have a fraud prevention department | 21:08 |
| kanzure | it's just a ruse | 21:08 |
| Burninate | yeah, I did that beforehand | 21:08 |
| Burninate | only time I've ever flagged my card was when I got too frisky on a Steam sale | 21:09 |
| kanzure | heh 500 u[B[Bheh 500 yuan | 21:09 |
| kanzure | irc failure | 21:09 |
| gradstudentbot | Well, I can't really talk about it because I'm trying to get it published in Science or Nature. | 21:10 |
| Burninate | the limits varied, some were 2000, some were unlimited | 21:10 |
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| drewbug | http://www.nbcnews.com/science/cloned-cats-glow-6C10405337 | 21:49 |
| drewbug | Would it be possible to do that to a human? | 21:49 |
| drewbug | A currently living human, that is. | 21:49 |
| kanzure | sigh | 21:49 |
| drewbug | Sorry if it's been beaten to death. | 21:49 |
| kanzure | well, the cats have been | 21:49 |
| Lemminkainen | possible? yes | 21:49 |
| Lemminkainen | practical? maybe | 21:49 |
| Lemminkainen | cheap? nope | 21:50 |
| kanzure | human glowth is just a natural extension that will also be beaten to death and it will annoy everyone... someone go get stelarc. | 21:50 |
| kanzure | damn, humanglowth.com is available | 21:50 |
| drewbug | gTLDs are coming soon, maybe we can get human.glowth | 21:51 |
| gradstudentbot | The culture got contaminated. | 21:52 |
| Lemminkainen | run it again, gradstudentbot | 21:52 |
| gradstudentbot | I need to send that abstract. | 21:52 |
| Lemminkainen | no you don't, go make some fucking data | 21:52 |
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| drewbug | Why has nobody glowth'd themselves yet? | 22:02 |
| drewbug | And what makes it expensive to do? | 22:04 |
| Lemminkainen | well what vector would you like to use? | 22:05 |
| drewbug | The best one for the job. | 22:05 |
| Lemminkainen | that's debatable | 22:05 |
| Lemminkainen | but let's assume you want to make your skin glow, so you go with a liposomal delivery medium | 22:06 |
| Lemminkainen | say, Lipofectamine-3000 | 22:06 |
| Lemminkainen | it runs about $2500/kit for any appreciable amount, and you'll need many kits | 22:06 |
| Lemminkainen | but on top of that, you're going to need to transfect a lot of genetic material as well | 22:06 |
| drewbug | What genetic material would that be? | 22:07 |
| Lemminkainen | if you want permanent glowth, you'll need to transfect your skin cells and, more importantly, your basement membrane cells with a specially designed CRISPR or TALEN driven by a constantly on promoter | 22:07 |
| Lemminkainen | there's an open-source TALENs method called Golden Gate that you could use to design template, order that in, and then grow it in mass | 22:08 |
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| Lemminkainen | then you'd have to purify it en masse (make sure to check against endotoxin) and get it into the Lipofectamine kits | 22:08 |
| Lemminkainen | and after that you'd get to rub it all over your skin, preferably by taking a gentle salt bath and remaining there for 6 hours or more | 22:09 |
| drewbug | And then you glow forever? | 22:09 |
| Lemminkainen | thing is, Lipofectamine is fairly shit at transfection efficiency, so the first time you do this you might get 20% efficacy | 22:10 |
| Lemminkainen | and a lot of dead skin cells (Lipofectamine-3000 just dropped this week with the headline "NOW LESS TOXIC") | 22:10 |
| Lemminkainen | it's still fairly toxic | 22:10 |
| drewbug | What are the other delivery vectors? | 22:10 |
| Lemminkainen | so you'd get to do repeated administrations of your glowey lipofectamine and hope that it keeps working without causing too much pain | 22:10 |
| Lemminkainen | yay | 22:10 |
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| Lemminkainen | there are also adenoviruses, adeno-associated viruses, and a few other viral vectors | 22:11 |
| Lemminkainen | but they tend to come with some unpleasantly inflammatory side-effects and have a bad habit of evolving and shit | 22:11 |
| drewbug | "inflammatory"? | 22:11 |
| Lemminkainen | inflammatory = your immune system reacts | 22:11 |
| kanzure | there is a lot of history of humans getting compromised immune systems because viruses are very good at their function | 22:12 |
| kanzure | inflammatory side-effects include death | 22:12 |
| Lemminkainen | in extreme cases, yes | 22:12 |
| kanzure | although the lentiviral vectors seem like an okay candidate for not killing you | 22:12 |
| Lemminkainen | but that hasn't stopped the EMA from approving Glybera (an AAV) for market | 22:12 |
| kanzure | which one is EMA? | 22:13 |
| Lemminkainen | European Medical Association | 22:13 |
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| Lemminkainen | Glybera treats thalassemia for about $1.1M/treatment | 22:13 |
| @kanzure | damn i can't come up with an EMA joke for /topic | 22:14 |
| @kanzure | federal death administration is staying for now | 22:14 |
| Lemminkainen | I think I like the idea of this channel being sponsored by George Church's beard better than Church hisself | 22:14 |
| @kanzure | well the whole point is that george church sponsors everything | 22:15 |
| @kanzure | he probably doesn't even know his complete list | 22:15 |
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| Lemminkainen | I'd like it even better if Feiyang and Hammond would grow beards as part of being included in Editas | 22:15 |
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| @kanzure | so who's to say he's not thinking fondly of hplusroadmap? | 22:16 |
| Lemminkainen | anyway drewbug there's also a bunch of polymer-DNA complex delivery mechanisms as well, but as the blasted patent landscape of the PEGylation craze shows, it works for shit | 22:16 |
| Lemminkainen | I suppose you could try suspending TALENs directly in DMSO and hoping that that works | 22:16 |
| drewbug | Hmm. | 22:16 |
| Lemminkainen | kanzure I hope he masturbates to hplusroadmap | 22:17 |
| @kanzure | uh, okay? | 22:17 |
| Lemminkainen | because that would be hilarious | 22:17 |
| drewbug | Skin cells and basement membrane cells. | 22:17 |
| drewbug | Could you do others? | 22:17 |
| Lemminkainen | depends on how you want to get to other cells | 22:18 |
| Lemminkainen | luciferase/luciferin transfection of buccal cells would be neat, then you'd have red-glowth saliva | 22:18 |
| gradstudentbot | Oh that's interesting, do you want to write a paper together? | 22:19 |
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| drewbug | I like that. | 22:19 |
| drewbug | Why red? | 22:19 |
| drewbug | You could do others, right? | 22:19 |
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| @kanzure | gradstudentbot: aren't you already coauthoring like 40 other papers? | 22:21 |
| gradstudentbot | That's definitely a Nature paper. | 22:21 |
| drewbug | Lol. | 22:21 |
| Lemminkainen | http://www.promega.de/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/?__utma=1.1542101436.1390544448.1390544448.1390544448.1&__utmb=1.1.10.1390544448&__utmc=1&__utmx=-&__utmz=1.1390544448.1.1.utmcsr=google|utmccn=(organic)|utmcmd=organic|utmctr=(not%20provided)&__utmv=-&__utmk=233469086 | 22:21 |
| Lemminkainen | there you go drewbug | 22:21 |
| @kanzure | that is the worst url ever | 22:21 |
| @kanzure | what the fuck promega | 22:21 |
| Lemminkainen | hey, they make molecules, not websites | 22:21 |
| Lemminkainen | (or customer support) | 22:21 |
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| @kanzure | it's like they attempted to do seo but then it backfired when they realized none of their customers use the internet | 22:21 |
| drewbug | kanzure: http://www.promega.de/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/ works just as well | 22:21 |
| @kanzure | yeah because when i'm searching for a promoter, "product guide" is in my query | 22:22 |
| @kanzure | yeesh | 22:22 |
| Lemminkainen | ain't worth your worry | 22:22 |
| drewbug | Lemminkainen: Thank you for the link. | 22:23 |
| @kanzure | it's something that could be done better | 22:23 |
| @kanzure | and also, promega itself makes a killing | 22:23 |
| @kanzure | so leaner entities can probably eat its lunch | 22:23 |
| Lemminkainen | no problem drewbug | 22:23 |
| Lemminkainen | and lately I am such a leaner entity | 22:23 |
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| drewbug | Lemminkainen: Could you do luciferase/luciferin transfection of skin cells? | 22:35 |
| drewbug | And the basement membrane cells. | 22:35 |
| drewbug | Instead of fluorescence. | 22:35 |
| Lemminkainen | that's what I was saying | 22:36 |
| drewbug | Oh. | 22:36 |
| drewbug | Cool. | 22:36 |
| drewbug | Thank you. | 22:37 |
| gradstudentbot | Huh, this western worked on the first try. | 22:39 |
| drewbug | Lemminkainen: Hypothetically, if you succeeded, could you then undo it? | 22:44 |
| Lemminkainen | by doing the same thing with an antisense transfection, yes | 22:50 |
| drewbug | Are they any downsides other than cost and being hated by everyone? | 23:02 |
| drewbug | When using the Lipofectamine method, that is. | 23:02 |
| Lemminkainen | toxicology | 23:07 |
| Lemminkainen | Lipofectamine has a bad habit of killing off the cells you're trying to transfect | 23:07 |
| Lemminkainen | problem is, so does almost everything else: GeneJuice, JetPEI, Invivofectamine | 23:08 |
| drewbug | Chance of death is very low, though? | 23:08 |
| Lemminkainen | leghlaHchu'be'chugh mIn lo'laHbe' taj jej | 23:09 |
| Lemminkainen | "a sharp knife is nothing without a sharp eye" | 23:10 |
| drewbug | I do not understand. | 23:10 |
| Lemminkainen | Klingon proverbs aside, the mortality risk is low | 23:10 |
| Lemminkainen | but morbidity risk is high | 23:10 |
| drewbug | What kind of morbidity? | 23:10 |
| Lemminkainen | skin death | 23:11 |
| drewbug | Would it not grow back? | 23:12 |
| * Lemminkainen shrugs | 23:12 | |
| Lemminkainen | that depends on how good a job you did at transfecting it | 23:12 |
| Lemminkainen | if you get off-site mutagenesis of the basement membrane there might be some major problems | 23:14 |
| Lemminkainen | so best to try a test patch somewhere first | 23:14 |
| drewbug | What do you mean by that? | 23:14 |
| drewbug | "a test patch"? | 23:15 |
| Lemminkainen | a small area of your skin, somewhere not too noticeable, not too flexible, that you can try the transfection on first before slathering it over all of your skin | 23:19 |
| drewbug | Wait, so, it wouldn't spread? | 23:20 |
| drewbug | Could I do it on just one finger? | 23:20 |
| Lemminkainen | yup | 23:20 |
| Lemminkainen | if you're using a liposomal or polymeric transfection methodology, it won't spread past where you apply it (much) | 23:21 |
| drewbug | And it'd still be permanent? | 23:21 |
| Lemminkainen | if you get to the skin stem cells, yeah | 23:21 |
| drewbug | How does one get to the skin stem cells? | 23:22 |
| Lemminkainen | basement membrane penetration | 23:22 |
| drewbug | Gotcha. | 23:22 |
| gradstudentbot | Why did I go to grad school? | 23:23 |
| gradstudentbot | Yeah, but biobricks don't even work. | 23:23 |
| drewbug | Would it be possible (and, if so, practical) to hook it up to a "trigger"? | 23:35 |
| drewbug | A neurological one, perhaps. Or, muscle use. | 23:35 |
| gradstudentbot | I'll be at the microscope. | 23:37 |
| Lemminkainen | ish | 23:37 |
| Lemminkainen | optogenetic triggers are getting better | 23:37 |
| Lemminkainen | a muscle- or neuro-trigger would be more difficult | 23:37 |
| drewbug | Optogenetic wouldn't lead to a feedback loop? | 23:38 |
| Lemminkainen | depends on the frequency of light | 23:38 |
| Lemminkainen | but then, it's just making light, not insulin, so no problem if there does wind up being a loop | 23:38 |
| drewbug | How long would it last if it were an optogenetic trigger? | 23:39 |
| gradstudentbot | How many papers do you have published? | 23:39 |
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| Lemminkainen | the optogenetic trigger would just be "turn on" | 23:42 |
| Lemminkainen | the genetic programming would be permanent if you do it right | 23:42 |
| drewbug | I mean, how long would it stay turned on? | 23:42 |
| Lemminkainen | you could also build in a "turn off" switch | 23:42 |
| drewbug | Or would you need a turn off | 23:42 |
| drewbug | Oh | 23:42 |
| Lemminkainen | as long as you left it on | 23:42 |
| drewbug | Okay. | 23:42 |
| drewbug | Huh. | 23:42 |
| drewbug | Very, very, very cool. | 23:43 |
| drewbug | Two different light frequencies, one for off and one for on? | 23:43 |
| Lemminkainen | yup | 23:43 |
| Lemminkainen | Ed Boyden's group has done that with neurons | 23:43 |
| drewbug | Would happens if both happen? | 23:43 |
| Lemminkainen | null | 23:43 |
| drewbug | *What | 23:43 |
| drewbug | Interesting. | 23:44 |
| Lemminkainen | should be noted I have a vested interest in this | 23:44 |
| drewbug | Oh? | 23:44 |
| Lemminkainen | I'm gearing up to release some really good transfection reagents this summer | 23:45 |
| drewbug | Expensive? | 23:45 |
| Lemminkainen | looking like it will have to be until they're sufficiently idiotproofed | 23:45 |
| drewbug | Any idea on pricing? | 23:46 |
| Lemminkainen | the math we've done so far for them suggests about $700 for a basic kit | 23:46 |
| drewbug | Awesome! | 23:47 |
| drewbug | What would something like this need? | 23:47 |
| Lemminkainen | 1 kit should be enough to fully transfect 1 mouse | 23:47 |
| drewbug | So, a bunch. | 23:47 |
| Lemminkainen | besides the kit itself you'd need some fancy salts and fancy waters and pipettes to actually do the transfection | 23:47 |
| drewbug | Fancy pipettes? | 23:47 |
| drewbug | Oh | 23:48 |
| drewbug | I guess you didn't say that. | 23:48 |
| drewbug | Pipettes? | 23:48 |
| Lemminkainen | micropipettes | 23:48 |
| Lemminkainen | standard molecular biology tool | 23:48 |
| drewbug | What are they needed for, though? | 23:48 |
| Lemminkainen | very precisely and sterilely transferring tiny amounts of expensive liquids | 23:49 |
| drewbug | What step of the process would that be? | 23:49 |
| Lemminkainen | getting your plasmid on the inside of the transfection-enabling bits | 23:49 |
| --- Log closed Fri Jan 24 00:00:00 2014 | ||
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