| --- Log opened Tue Jun 17 00:00:37 2014 | ||
| AshleyWaffle | ebowden: yeah but whats the best one to tackle first | 00:00 |
|---|---|---|
| ebowden | Don't know. | 00:01 |
| AshleyWaffle | well my idea is | 00:01 |
| AshleyWaffle | combining lucid dreaming or daydreaming with this | 00:01 |
| ebowden | This is likely one of those things that gets better as advancements accumulate. | 00:01 |
| AshleyWaffle | you could make media of any sort | 00:02 |
| ebowden | There's probably not a magic research bullet. | 00:02 |
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| AshleyWaffle | or do a video in the style of video game lets plays | 00:02 |
| AshleyWaffle | hey augur | 00:02 |
| Daeken | kanzure: pong | 00:03 |
| archels | AshleyWaffle: mostly, recording resolution | 00:09 |
| archels | but before you do any of these experiments you have to learn the mapping, which means exposing the subject to countless images and simultaneous measuring their brain activity | 00:10 |
| AshleyWaffle | yep | 00:12 |
| AshleyWaffle | how practical would this be to diy | 00:12 |
| AshleyWaffle | with a budget of, for example, $1k | 00:12 |
| archels | uh, I'm not sure what refurbished MRI scanners go for, but I'm willing to bet it's a little over $1k | 00:13 |
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| AshleyWaffle | archels: and the resolution would be? | 00:15 |
| AshleyWaffle | probably not full resolution 1080p of your dreams, heh | 00:15 |
| archels | oh, when you say 'resolution' do you refer to the resolution of the reconstructed 'dream images'? | 00:16 |
| AshleyWaffle | archels: the accuracy and precision of the reconstruction anyway | 00:20 |
| AshleyWaffle | with an ideal scenario looking as if watching a movie or recording of real life | 00:20 |
| AshleyWaffle | and minimum effectiveness for satisfying me if i were a consumer would be easy to make out whats going on | 00:20 |
| AshleyWaffle | but shitty quality and odd distortions | 00:20 |
| archels | the reconstruction is just a weighted sum of the individual images that were presented during the establishment of the map, so they could be 4k or whatever | 00:21 |
| archels | the prettiness of the reconstruction is limited by how many images were used to esablish time map, which I guess is mostly a time/practicality constraint, but most importantly the resolution of the brain scan (fMRI voxel size) | 00:22 |
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| Stevko | paperbot: http://www.nature.com/nature/journal/v476/n7361/abs/476393a.html | 04:16 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2F476393a | 04:16 |
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| eudoxia | 23:12 < kanzure> when i was thinking about refactoring it, i was thinking of leaving the GUI behind | 06:19 |
| eudoxia | 23:12 < kanzure> and just doing a small programming tool | 06:19 |
| eudoxia | kanzure: how would that actually work though? NE-1 lets you do things like create big blocks of uniform diamond | 06:19 |
| eudoxia | then with the GUI would can, say, place a Ge dimer on the center of one of the faces. how would you do that with some kind of chemistry DSL? | 06:20 |
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| eudoxia | s/would can/you can | 07:06 |
| gradstudentbot | Yeah, but his project was so easy. | 07:07 |
| chris_99 | paperbot: http://link.springer.com/chapter/10.1007%2F978-94-007-5170-5_14 | 07:13 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/399ab308bed84d4c8e5fb731fb2d8d4d.txt | 07:14 |
| kanzure | well, you would just pick which atom you want | 07:33 |
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| kanzure | i bet you can't really pick the center unless it's an odd number | 07:34 |
| kanzure | or you can split at half-way i guess | 07:34 |
| eudoxia | i guess something like atoms.select(x=max, y=height/2, z=depth/2) or something but that could get really complex and not at all accurate | 07:36 |
| kanzure | well, programmatic macro-cad works, why not atomcad? | 07:38 |
| eudoxia | because it's simple as long as you're either creating all the atoms manually (or through explicit iteration or something) or creating chunks of atoms in bulk, but mixing the too seems like a hard problem | 07:39 |
| kanzure | shrug | 07:39 |
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| kanzure | it sounds like gene_hacker finds it more useful for the pointyclicky reasons | 07:40 |
| eudoxia | that's why it should have a GUI | 07:40 |
| gradstudentbot | My pub of choice is pubmed. | 07:40 |
| eudoxia | but, as you said, even the internal representation of molecules is done by another library, so it might just be easier to rewrite the whole thing | 07:40 |
| kanzure | internal representation of molecules is nanoengineer | 07:41 |
| eudoxia | i thought it was openbabel | 07:41 |
| kanzure | haha no | 07:41 |
| eudoxia | i'm pretty sure it's designed to do that and since NE-1 uses it | 07:41 |
| kanzure | pffffft standardization | 07:41 |
| eudoxia | well whatever, there's ChemPy https://github.com/jwallen/ChemPy | 07:44 |
| kanzure | that looks way more clean | 07:46 |
| kanzure | eudoxia, does that do the things adequately? | 07:58 |
| eudoxia | kanzure: i have not used it, but it seems adequately tested | 08:00 |
| kanzure | well, go use it, you slacker | 08:01 |
| kanzure | try converting some stuff from https://github.com/kanzure/nanoengineer/tree/master/cad/partlib | 08:01 |
| eudoxia | is there some not-mmp format that allows representing stuff like rotary and linear motors | 08:02 |
| eudoxia | sparsely documented project-specific formats are not a good thing | 08:02 |
| kanzure | pdb? heh dunno | 08:02 |
| eudoxia | i know pdb has a boatload of extra features but it's more oriented towards protein stuff | 08:03 |
| gradstudentbot | Protip: I wouldn't go to the far end of the lab alone. | 08:03 |
| kanzure | is there seriously no molecule file format | 08:03 |
| kanzure | HULK SMASH | 08:03 |
| eudoxia | the pdb format has an entire section of records for classifying different parts of the molecule but no "this moves back and forth every 200 femtosecond" | 08:05 |
| eudoxia | femtoseconds* | 08:06 |
| kanzure | .title https://www.youtube.com/watch?v=XhQDxralP70 | 08:06 |
| yoleaux | Syringe pump first Testrun | 08:06 |
| gradstudentbot | Hey, I got 100% yield! Oh wait, no. | 08:07 |
| kanzure | .title https://www.youtube.com/watch?feature=player_embedded&v=OtR9y1TYoXc | 08:09 |
| yoleaux | PDMS plasma surface treatment in improvised plasma chamber | 08:09 |
| kanzure | syringe pump in action https://plus.google.com/photos/109143331381566436146/albums/6022970869991461601/6022970870156503138?pid=6022970870156503138&oid=109143331381566436146 | 08:10 |
| kanzure | oh i guess this is the thing cathal garvey is paying for (synbioaxlr8r) | 08:11 |
| kanzure | "Just wanted to invite you to following along the development of our recent project, which is a dna synthesizer named KiloBaser. It's based on microfluidic and magnetic technology, open source and low cost. There are still challenges to overcome, but we are quite convinced to have a basic prototype soon. Our team started at the biohackerspace 'open biolab graz austria', currently working in Ireland for the summer." | 08:12 |
| kanzure | by "open source" they mean "we fuck you in the ass by making bryan click facebook/twitter links" | 08:12 |
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| kanzure | joepie91_: pdfstuff? | 08:18 |
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| joepie91_ | ffs | 08:44 |
| joepie91_ | washed cheese grater a little too aggressively | 08:44 |
| joepie91_ | it bit back >.> | 08:44 |
| joepie91_ | kanzure: likely pdfstuffhappen this weekend | 08:44 |
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| kanzure | hm | 09:35 |
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| FourFire | kanzure, what's your opinion on Aubrey De Grey ? | 10:07 |
| FourFire | He's doing an AMA in an hour | 10:07 |
| kanzure | he spends too much time on marketing, otherwise good opinion | 10:08 |
| kanzure | i suggest you read al of these in the next hour: http://diyhpl.us/~bryan/papers2/longevity/ | 10:09 |
| kanzure | and some of his: http://diyhpl.us/~bryan/papers2/longevity/Aubrey/ | 10:09 |
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| ParahSailin | hes always drunk | 10:19 |
| kanzure | which is not necessarily a bad thing | 10:20 |
| justanotheruser | kanzure: have you even read all of those? O_o | 10:22 |
| kanzure | there's only 382 papers in there | 10:23 |
| nmz787_i | kanzure: i don't think i saw his email in your reply | 10:27 |
| kanzure | it was through ebay | 10:27 |
| FourFire | kanzure, well I'm hoping to ask some leading questions As soon as the AMA gets posted | 10:49 |
| chris_99 | is it on reddit? | 10:50 |
| ParahSailin | no reddit | 10:50 |
| chris_99 | AMA is a reddit thing mainly, right? | 10:50 |
| eudoxia | we never get any cool AMAs | 10:50 |
| kanzure | why would you ask leading questions ugh | 10:52 |
| FourFire | chris_99, yeah it's supposed to be on /r/futurology, in about an hour | 10:52 |
| chris_99 | cool | 10:53 |
| FourFire | (I glooped on the time) | 10:53 |
| chris_99 | Does Aubrey work at Cambridge? | 10:53 |
| kanzure | aubrey lives on the road giving presentations | 10:54 |
| chris_99 | heh | 10:54 |
| FourFire | kanzure, maybe that's the most effective thing he can do | 10:54 |
| eudoxia | well you know at least he's convincing people death = bad | 10:55 |
| eudoxia | ==* | 10:55 |
| kanzure | that's a rather pathetic limit to place on yourself | 10:55 |
| kanzure | consider he did okay theory work on aging in the past | 10:55 |
| kanzure | not the greatest, but not shit either | 10:55 |
| eudoxia | there are still too many deathists in the world | 10:55 |
| FourFire | towards actually getting enough people in the know and serious about solving things | 10:55 |
| eudoxia | yes i hate using that word | 10:55 |
| FourFire | I'm going to ask him about that actually | 10:55 |
| kanzure | "let's just get enough attention and hope that at some point we stumble into someone that knows the answers" is a *bad* stratey | 10:56 |
| kanzure | *bad strategy | 10:56 |
| FourFire | kanzure, well the field is rather underfunded compared to, say baldness | 10:56 |
| kanzure | who cares? aubrey's rich as fuck | 10:56 |
| kanzure | well, not as fuck | 10:56 |
| kanzure | at least $10 million | 10:56 |
| FourFire | it's about brute forcing the problems too, not just "finding someone who knows the answer" or whatever | 10:57 |
| kanzure | actually, a lot of options are often taken off the table because they seem too strange | 10:57 |
| ParahSailin | aubrey doesnt live at cambridge afaik | 10:57 |
| FourFire | we need a bunch of intelligent scientists working on systematically producing solutions to these interconnected systematic failure modes | 10:58 |
| kanzure | for example, people are willing to cryogenically preserve their heads but they aren't willing to do whole body transplants or brain-only life support | 10:58 |
| eudoxia | most people aren't willing to do that | 10:58 |
| FourFire | well brain life support seems like death | 10:58 |
| FourFire | sensory deprivation and that | 10:58 |
| eudoxia | lol whut | 10:58 |
| kanzure | whether or not you need help it is still insulting to say "maybe conference presentations are the best he can do"- considering he has given evidence that he is capable of doing other work | 10:58 |
| FourFire | whole body transplants is very morally D: | 10:58 |
| eudoxia | how is it morally anything | 10:59 |
| FourFire | but people willing to cryo are a tiny minority | 10:59 |
| kanzure | why are you always so broken | 10:59 |
| kanzure | like what the hell happened to you | 10:59 |
| kanzure | i used to think it was all the stupid parts of lesswrong leaking into you, but now i'm not sure | 11:00 |
| FourFire | kanzure, my intellectual deficiencies aside, it's not just underfunded, it's probably also under appreciated: people donæt know it's a thing | 11:00 |
| kanzure | i am not claiming deficiencies | 11:00 |
| FourFire | what did I say? | 11:01 |
| kanzure | "my intellectual deficiencies aside," | 11:01 |
| FourFire | for a whole body transplant someone has to die for each person that lives, right? | 11:01 |
| seba- | yes | 11:01 |
| seba- | but you can just make 7 clones of you | 11:01 |
| FourFire | and brains suffer disease too | 11:02 |
| seba- | and harvest their organs | 11:02 |
| chris_99 | ParahSailin, ah i was wondering if he gave any lectures i could frop into | 11:02 |
| chris_99 | *drop even | 11:02 |
| kanzure | seba-: unfortunately those clones are too young to matter, often | 11:02 |
| kanzure | i think i already corrected myself and said brain-only support | 11:02 |
| FourFire | clones is a good point, but again we get into the moral ickyness because people are stupid about these things | 11:02 |
| seba- | kanzure, yeah but you grow them in advance | 11:02 |
| kanzure | iirc there's a term that sounds like "whole-body transplant" but is related to whole head removal | 11:02 |
| kanzure | it's the one where you don't kill a second person, and where you can't use the original body | 11:02 |
| kanzure | except for the head | 11:02 |
| FourFire | you mean like the russian robot dog experiments? | 11:03 |
| kanzure | no | 11:03 |
| kanzure | just normal brain in a jar stuff | 11:03 |
| kanzure | with perfusion | 11:03 |
| eudoxia | http://en.wikipedia.org/wiki/Head_transplant "The consensus on the ethics of such a procedure is negative." | 11:04 |
| eudoxia | ahahahah bioethics would be such a fucking joke if it wasn't so dangerous | 11:04 |
| seba- | FourFire could you try applying 30 V at certain parts of your body and see if you shit yourself? | 11:04 |
| seba- | i mean i would show you which points | 11:04 |
| seba- | as per patent | 11:04 |
| chris_99 | is that your new fetish seba- ;) | 11:04 |
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| seba- | chris_99, http://www.google.com/patents/US3941136 | 11:05 |
| chris_99 | i daren't not look heh | 11:05 |
| gradstudentbot | Is this going to count as my ethics training? | 11:08 |
| kanzure | "I have a quite a bit of experience with organic synthesis, especially the synthesis of complex polyketide type natural products, feel free to ask me any questions." | 11:08 |
| joepie91_ | kanzure: do you have a paper handy with an image watermark? I think I just figured out how to do the architecture of pdfparanoia, but I want to check to be sure that it also catches that usecase | 11:12 |
| joepie91_ | (preferably two papers with an image watermark from the same source) | 11:12 |
| joepie91_ | all the papers on the bugtracker seem to be text, unless I missed one | 11:12 |
| kanzure | there are samples in the tests folder | 11:12 |
| kanzure | i think IOP uses some images on the first page | 11:13 |
| * joepie91_ goescheck | 11:13 | |
| kanzure | "institute of physics" | 11:13 |
| joepie91_ | per https://github.com/kanzure/pdfparanoia/issues/3 that seems to be text | 11:14 |
| joepie91_ | not image :/ | 11:14 |
| kanzure | looking | 11:14 |
| kanzure | nmz787_i: http://diyhpl.us/~bryan/papers2/DNA/abi391/ | 11:15 |
| joepie91_ | kanzure: also, the AIP test paper has an advertisement - should it strip that also, or just explicit watermarks? | 11:15 |
| kanzure | that is a good question | 11:15 |
| joepie91_ | it depends on whether you want the PDFs to be "cleaned" or just "anonymized" | 11:16 |
| joepie91_ | I suppose | 11:16 |
| kanzure | well, it's certainly the same *type* of operation | 11:16 |
| kanzure | it should be stripped, because they might have a record of which ads were shown on which papers | 11:16 |
| kanzure | for which users that downloadecd | 11:16 |
| joepie91_ | also, none of those in tests/ appear to employ image watermarks | 11:17 |
| joepie91_ | right, that was a side-thought of mine as well | 11:17 |
| joepie91_ | I'll go with "yes, should be stripped" then :P | 11:17 |
| kanzure | i believe i saw an image watermark once, but it was just a 180x20 pixel thing, in the same location on every page | 11:17 |
| kanzure | i just don't remember which publisher :( | 11:17 |
| joepie91_ | was it unique? | 11:17 |
| joepie91_ | or just a logo kind of thing? | 11:17 |
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| kanzure | had a variable piece of information | 11:17 |
| joepie91_ | ah | 11:17 |
| kanzure | it was either institution name, date, or ip address | 11:17 |
| joepie91_ | if you run across it, can you link me to it? | 11:17 |
| kanzure | yep | 11:18 |
| kanzure | "notice of extortion" https://i.imgur.com/cG07rQU.jpg | 11:20 |
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| kanzure | ParahSailin: we should probably start making a list of primers worth synthesizing | 11:30 |
| ParahSailin | what | 11:30 |
| kanzure | because we have a synthesizer now | 11:31 |
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| ParahSailin | who has a synthesizer | 11:32 |
| kanzure | i bought it last night, shipping it to nmz787 | 11:32 |
| ParahSailin | ah which | 11:32 |
| kanzure | ABI 391 | 11:32 |
| ParahSailin | why wouldnt i order from idt | 11:33 |
| kanzure | shrug, you're free to | 11:33 |
| ParahSailin | is marginal cost cheaper with yours? | 11:33 |
| kanzure | haven't calculated yet | 11:34 |
| ParahSailin | why you sent it to him and not me? | 11:35 |
| kanzure | because i'm a fucking idiot | 11:35 |
| kanzure | pick one out and i'll get it | 11:37 |
| seba- | why TLC is not used much in biochem? | 11:37 |
| ParahSailin | what you mean that paper thing? | 11:37 |
| seba- | no | 11:37 |
| ParahSailin | er, the plates i mean | 11:37 |
| seba- | thin layer chromatography | 11:37 |
| seba- | plates yes | 11:37 |
| ParahSailin | because electrophoresis | 11:38 |
| seba- | yes sure | 11:38 |
| seba- | but TLC is faster | 11:38 |
| seba- | and more convinient | 11:38 |
| ParahSailin | i have never heard of tlc for large molecules | 11:38 |
| ParahSailin | fuck, and convenient is not a word i would associate with tlc | 11:38 |
| seba- | why not | 11:39 |
| ParahSailin | fucking scoring plates and the huge glass box full of chloroform in the hood | 11:39 |
| seba- | huh? | 11:39 |
| seba- | what? | 11:39 |
| seba- | lol | 11:39 |
| ParahSailin | tlc yo | 11:39 |
| seba- | why do you need chloroform | 11:39 |
| seba- | most plates or aluminum nowdays | 11:39 |
| ParahSailin | you always need some crazy mixture of solvents | 11:39 |
| ParahSailin | and you need to try like 10 different ones to see which gets you the best separation | 11:40 |
| seba- | chloroform is not used much, except in biochem protocols | 11:40 |
| seba- | well column chromatography it's used | 11:41 |
| ParahSailin | er... | 11:41 |
| ParahSailin | yeah | 11:41 |
| seba- | and also a lot of chromatography using centrifuge in various kits | 11:41 |
| seba- | i just can't see why not TLC | 11:41 |
| ParahSailin | i have never heard of tlc for large molecules | 11:41 |
| ParahSailin | do you know how the equations for diffusion go? | 11:42 |
| seba- | yes me neither, but i don't see why it wouldn't work | 11:42 |
| seba- | at least for proteins and such | 11:42 |
| seba- | http://onlinelibrary.wiley.com/doi/10.1002/bmc.1130030302/abstract | 11:43 |
| seba- | hm | 11:43 |
| gene_hacker | excellent | 11:44 |
| gene_hacker | we have a synth now? | 11:44 |
| ParahSailin | what part of slurrying cellulose powder in methanol-water and spread over glass plates and dried in vacuo overnight is "super faster and convenient" | 11:44 |
| seba- | ParahSailin you can buy those premade | 11:44 |
| seba- | they don't cost much | 11:45 |
| ParahSailin | lol ill stick to gels | 11:45 |
| seba- | yes but why | 11:45 |
| ParahSailin | the red dye makes it go fasta | 11:45 |
| seba- | i must experiment a bit with TLC and proteins, i think it could be good hm | 11:46 |
| kanzure | gene_hacker: yes | 11:47 |
| ParahSailin | fuck, i hate everything about TLC, you should have given me a trigger warning | 11:47 |
| seba- | ParahSailin why | 11:47 |
| ParahSailin | now i am re-living my worst days | 11:47 |
| seba- | what's so wrong about TLC | 11:47 |
| seba- | i don't get it | 11:47 |
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| FourFire | kanzure, I might have missed it, but what did i say? | 11:53 |
| FourFire | "<kanzure> why are you always so broken | 11:53 |
| FourFire | like what the hell happened to you | 11:53 |
| FourFire | i used to think it was all the stupid parts of lesswrong leaking into you, but now i'm not sure" | 11:53 |
| gene_hacker | https://medium.com/the-physics-arxiv-blog/first-movie-of-the-neuronal-activity-in-an-entire-brain-ab315e8ab013 | 11:53 |
| kanzure | your moral arguments against aubrey de grey doing anything | 11:54 |
| kanzure | .title https://www.youtube.com/watch?v=54xsgZWtWc8 | 11:54 |
| yoleaux | Widefield temporal focusing (WF-TEOF) of C.elegans brain for neuroscience. | 11:54 |
| kanzure | gene_hacker: did you see the CLEAR thing? | 11:55 |
| kanzure | .title https://www.youtube.com/watch?v=c-NMfp13Uug | 11:55 |
| yoleaux | See-through brains | 11:55 |
| kanzure | gene_hacker: that one | 11:55 |
| gene_hacker | so Brainbow 2: electric boogaloo? | 11:55 |
| kanzure | paperbot: http://arxiv.org/abs/1406.1603 | 11:56 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2Fnmeth.2637 | 11:56 |
| FourFire | kanzure, oh, I made moral arguments? | 11:57 |
| FourFire | uhh | 11:57 |
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| kanzure | "it is immoral for him not to be doing public promotion" | 11:57 |
| kanzure | etc | 11:57 |
| FourFire | I was pointing out that yes, he might be a very productive person working in the field, but it might be better if he tells ten other scientist people about the field existing, and maybe gets some rich people to fund things | 11:58 |
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| FourFire | I didn't say it's immoral for him to work in the field :( | 11:59 |
| kanzure | he *is* a rich person | 11:59 |
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| chris_99 | i've got a question for him, just how does he keep his beard so luscious | 12:03 |
| FourFire | kanzure, ok, but does 10 million go very far? | 12:03 |
| gene_hacker | beard wax does wonders | 12:04 |
| chris_99 | heh | 12:04 |
| kanzure | FourFire: depends on what you're doing | 12:04 |
| kanzure | using academic labs is very expensive because the universities take 58% of all incoming money | 12:05 |
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| FourFire | 58% ? | 12:08 |
| FourFire | that's a very specific number | 12:08 |
| kanzure | 52-58% usually | 12:09 |
| kanzure | somewhere around that range. it's never 50%. | 12:09 |
| ParahSailin | the beer constantly dripping into the beard acts as a moisturizer and sunscreen | 12:09 |
| chris_99 | haha | 12:09 |
| chris_99 | http://www.reddit.com/r/Futurology/comments/28e4v3/aubrey_de_grey_ama/ | 12:13 |
| FourFire | seems interesting: http://www.reddit.com/r/Futurology/comments/28e4v3/aubrey_de_grey_ama/cia1asc | 12:13 |
| FourFire | ... | 12:14 |
| chris_99 | haha he's got his favourite beer style on thar | 12:16 |
| ParahSailin | dont get me wrong, i dont think hes an alcoholic | 12:16 |
| kanzure | wouldn't be too bad if he was, anyway | 12:17 |
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| ParahSailin | but when you are good enough, a constant diet of beer means you dont get drunk, and means you require very little solid food | 12:17 |
| ParahSailin | it is the original soylent | 12:17 |
| chris_99 | haha | 12:17 |
| ParahSailin | everyone is just catching up to adg | 12:17 |
| chris_99 | this google Calico thing sounds interesting | 12:18 |
| kanzure | most people don't need aubrey to tell them about calico since it was plastered all over the crapnews.. | 12:18 |
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| nmz787_i | 'The name Calico is shorthand for California Life Company' | 12:19 |
| JanSauerbier | the name geico | 12:23 |
| JanSauerbier | is shorthand for government insurance company | 12:23 |
| ParahSailin | government employee, yeah | 12:23 |
| JanSauerbier | fqn lizard people | 12:23 |
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| ParahSailin | people still talking about that azolla experiment? i thought it was already established that they are paying extra for the wrong kinds of sequencing | 13:05 |
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| kanzure | bullshit diffuses faster than people stop to wonder if anyone has already identified the bullshit | 13:08 |
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| nmz787_i | ParahSailin: no one mentioned that on diybio | 13:23 |
| ParahSailin | ah, well its in the comments of that crowdfunding page | 13:23 |
| nmz787_i | oh | 13:23 |
| nmz787_i | didn't look that far | 13:23 |
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| nmz787_i | saw that their prizes were retarded and they were all academics | 13:24 |
| nmz787_i | just seemed like they got super lazy | 13:24 |
| nmz787_i | so i was dissuaded quite fast | 13:24 |
| ParahSailin | basically, a 300 cycle illumina run will have utter shit error rate | 13:24 |
| ParahSailin | and they should do the cheaper, more data 200 cycle PE run | 13:25 |
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| ParahSailin | biologists should not be allowed to design these experiments | 13:28 |
| kanzure | paperbot: http://pubs.rsc.org/en/content/articlelanding/2011/lc/c0lc00550a | 13:28 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/Micromolding%20of%20solvent%20resistant%20microfluidic%20devices.pdf | 13:29 |
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| kanzure | what are those rubber jar caps called where you can insert a needle without exposing the contents to the environment? | 13:49 |
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| ParahSailin | vial | 13:54 |
| kanzure | how does it prevent leakage? | 13:55 |
| ParahSailin | crimpage | 13:56 |
| kanzure | can you find me a diagram | 14:00 |
| nmz787_i | kanzure: brandname is SureSeal | 14:02 |
| kanzure | http://www.ncbi.nlm.nih.gov/pubmed/12353585 "n the early 1990s, the microscopy and microanalysis group of the U.S. Food and Drug Administration's Forensic Chemistry Center (FCC) developed and implemented a method (unpublished) to locate needle punctures in rubber pharmaceutical vial stoppers." | 14:02 |
| nmz787_i | kanzure: otherwise they're septa bottle tops/lids | 14:02 |
| nmz787_i | rubber septa | 14:02 |
| kanzure | so the holes are still there | 14:02 |
| nmz787_i | yeah, just all smoosed up | 14:03 |
| kanzure | smoosed? | 14:03 |
| nmz787_i | smooshed | 14:03 |
| kanzure | well yes but how? | 14:03 |
| nmz787_i | its like smashed | 14:03 |
| nmz787_i | umm | 14:03 |
| nmz787_i | just how rubber works | 14:03 |
| kanzure | magic | 14:03 |
| kanzure | hm | 14:03 |
| nmz787_i | it has some limited flow | 14:03 |
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| kanzure | "Transmission electron microscopy study of magnetites in a freshwater population of magnetotactic bacteria" http://www.impmc.jussieu.fr/~menguy/PDFs/Articles/Isambert_AmMin_2007.pdf | 14:26 |
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| nmz787_i | yay for online ESD training! | 14:41 |
| nmz787_i | sortof | 14:41 |
| kanzure | ? | 14:41 |
| nmz787_i | 'When you feel that shockk! >=~3kV | 14:42 |
| nmz787_i | " | 14:42 |
| nmz787_i | hear it... ~5kV | 14:42 |
| nmz787_i | see it, ~8kV | 14:42 |
| FourFire | nmz787, but tiny current | 14:44 |
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| ThomasEgi | when your cmos device experiences ESD shock... you'll notice by getting sporadic weird unpredictable hard to debug errors. | 14:45 |
| nmz787_i | hmm, even an undischarged field can induce damage | 14:48 |
| nmz787_i | ThomasEgi: you have experienced a 'Latent Failure' | 14:49 |
| nmz787_i | 90% of all failures fall into that category | 14:49 |
| nmz787_i | ungroundable non-conductors should reamain 18" away from device | 14:50 |
| nmz787_i | hmm | 14:51 |
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| nmz787_i | guess i need a grounding mat for my desk | 14:51 |
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| nmz787_i | wheely chairs: 18kV generators | 14:51 |
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| nmz787_i | paperwork should not come into direct contact with sensitive components | 14:52 |
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| kanzure | this is a good review of bacterial magnetotaxis http://www.calpoly.edu/~rfrankel/RBFAnnRevBio.pdf | 14:52 |
| nmz787_i | hmm, heel straps for your shoes | 14:53 |
| kanzure | interesting that they appear in most freshwater ponds | 14:53 |
| nmz787_i | too many ions elsewhere? | 14:53 |
| nmz787_i | in salt water i mean | 14:53 |
| kanzure | no, they appear in salt water as well | 14:54 |
| nmz787_i | hmm, ground monitors... complains if your wrist strap or desk mat goes ungrounded | 14:54 |
| nmz787_i | 'IC lotion' | 14:56 |
| nmz787_i | wrist and heel straps need tested daily | 14:58 |
| ThomasEgi | if you have unprotected inputs.. looking at it with a fritening look on your face might already be enough to cause damage ;) | 14:59 |
| ThomasEgi | laser diodes are the worst when it comes to that | 15:00 |
| kanzure | hm, there was a company that was going to use magnetosomes from bacteria as a magnetic tape (for data storage) | 15:00 |
| nmz787_i | http://www.ebay.com/itm/3M-722-ESD-Work-Station-Monitor-Anti-Static-w-730-Remote-Input-Jack-TESTED-Y51-/321400479192?pt=LH_DefaultDomain_0&hash=item4ad4f61dd8 | 15:01 |
| ThomasEgi | once build an amplifier with about 6gigaohm input impedance. it picked up strong signals from an old cathode tube tv several rooms away from mine. good thing it didn't cause any damage. | 15:01 |
| nmz787_i | hmm, selling from next town over | 15:01 |
| nmz787_i | 'guests only need 1 heel strap, unless they're going to handle devices, then they need 2 | 15:02 |
| nmz787_i | ' | 15:02 |
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| kanzure | "The magnetite crystals are typically 42 nm in diameter." | 15:03 |
| nmz787_i | metallic bags are only effective if there are no tears or holes and is zipped/folded closed | 15:05 |
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| kanzure | i have no idea what nmz787 is talking about | 15:05 |
| nmz787_i | single-layer bags are single-use only.. crinkles ruin the faraday cage properties | 15:06 |
| nmz787_i | kanzure: these https://svartizulu.files.wordpress.com/2014/03/metal-esd-bag.gif | 15:06 |
| nmz787_i | those are static-shielding | 15:06 |
| nmz787_i | these are anti-static (not for sensitive parts, for stuff like paper work orders, etc) http://uk.farnell.com/productimages/farnell/standard/42259127.jpg | 15:06 |
| kanzure | yes but how did this topic happen | 15:08 |
| kanzure | something about high heel straps? | 15:08 |
| nmz787_i | kanzure: diff is the metallic ones are like a faraday cage (external ESD won't get through)... the pink plastic bags don't generate charge from physical contact (i.e. balloon rubbed on your hair)... but externally applied ESD will penetrate | 15:08 |
| nmz787_i | i'm taking an online training | 15:08 |
| nmz787_i | I wonder if un-grounded vs grounded people experienced this differently https://en.wikipedia.org/wiki/Solar_storm_of_1859 | 15:10 |
| kanzure | gene_hacker: 42 nm magnetic nanoparticles, what cool things should be done with them | 15:11 |
| kanzure | besides magnetic resonance imaging | 15:12 |
| kanzure | "Magnetic nanoparticles can be used for a variety of genetics applications. One application is the isolation of mRNA. This can be done quickly – usually within 15 minutes. In this particular application, the magnetic bead is attached to a poly T tail. When mixed with mRNA, the poly A tail of the mRNA will attach to the bead's poly T tail and the isolation takes place simply by placing a magnet on the side of the tube and pouring out the ... | 15:13 |
| kanzure | ... liquid." | 15:13 |
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| ParahSailin | paperbot: http://psycnet.apa.org/journals/dev/50/3/881/ | 15:40 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/584b6e4a1faf949d769140d8292e211f.txt | 15:40 |
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| nmz787_i | 'in the event of an emergency, call 4-4444, DO NOT CALL 911' | 15:58 |
| nmz787_i | hmm | 15:58 |
| nmz787_i | I wonder if DIY labs should follow this | 15:58 |
| kanzure | 911 might be aware of that number | 15:59 |
| kanzure | ... probably not, nevermind. | 15:59 |
| nmz787_i | nah its an internal number | 16:05 |
| ParahSailin | campus popo | 16:06 |
| ParahSailin | their pbx switchboards are all configured the same way | 16:06 |
| kanzure | hmm i need to find a good su8/pdms/photoresist supplier | 16:10 |
| kanzure | http://microchem.com/ http://www.gersteltec.ch/ http://makerjuice.com/ http://www.tok.co.jp/en/products/ https://www.norlandprod.com/adhesives/ http://www.deco-coat.com/uv.html | 16:11 |
| kanzure | feels like i'm missing an obvious supplier | 16:11 |
| kanzure | nmz787_i: i have a chemistry consultant friend who is an expert at photoresists, epoxies and all things that cure, | 16:18 |
| kanzure | nmz787_i: i'm preparing an email to him since i haven't talked with him in a while | 16:18 |
| kanzure | nmz787_i: if you want to pile in any questions, let me know | 16:18 |
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| kanzure | hm, he is having surgery tomorrow, so will have lots of time to read | 16:28 |
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| nmz787_i | kanzure: hmm, idk right now... nothing comes to mind immediately | 16:43 |
| nmz787_i | kanzure: FYI in case you don't realize, that tok.co.jp company is literally right up the street from me | 16:43 |
| nmz787_i | from work its like turn out of parking lot, right onto evergreen, left onto brookwood, right into TOK parking lot | 16:44 |
| nmz787_i | and they've been quite helpful in email and on calls | 16:44 |
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| nmz787_i | http://opensourcebridge.org/events/2014/tracks | 17:14 |
| nmz787_i | Chemistry track | 17:14 |
| kanzure | azco biotech replied to that microfluidic dna synthesizer thread | 17:29 |
| nmz787_i | yeah | 17:52 |
| nmz787_i | the OP of that thread never replied to my last email to him | 17:52 |
| nmz787_i | so whatever | 17:52 |
| nmz787_i | which was like a month or more ago | 17:52 |
| kanzure | i am not convinced that their project is open source | 17:53 |
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| kanzure | they just have some stupid facebook pics or something | 17:53 |
| kanzure | "Early on in their life, Instacart couldn't convince grocery stories to give them access to product inventory lists or high-quality photos. So they found a cheap photography studio over a weekend, went to their local Trader Joe's to buy one of every single item and then spent 48 hours photographing all the inventory. Problem solved." | 17:53 |
| justanotheruser | sounds expensive | 17:56 |
| jmil | open access article: 3D Printing and the Billion Cell Construct http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001882 | 17:56 |
| jmil | kanzure: i hope you enjoy it ^^^ | 17:56 |
| jmil | :D | 17:56 |
| kanzure | paperbot: http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001882 | 17:57 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/The%20Billion%20Cell%20Construct%3A%20Will%20Three-Dimensional%20Printing%20Get%20Us%20There%3F.pdf | 17:57 |
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| nmz787_i | kanzure: he never responded after I sent him an idea based on the few things he told me | 18:20 |
| nmz787_i | kanzure: I wonder if my idea was too close to theirs | 18:20 |
| nmz787_i | or he thought I came off as too, uh, too strong? | 18:20 |
| kanzure | that's strange, considering they claim it's open source | 18:20 |
| kanzure | maybe we should ask cathal to clear up the situation | 18:20 |
| nmz787_i | meh | 18:20 |
| kanzure | hah | 18:21 |
| nmz787_i | their synbioaxlr8r didnt require it | 18:21 |
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| gene_hacker | kanzure: ferrofluid perhaps? | 18:21 |
| kanzure | i haven't played with a ferrofluid ever | 18:21 |
| gene_hacker | you should, it is quite entertaining | 18:21 |
| gene_hacker | with a bit of chemistry magic you might be able to make this: http://spie.org/x48084.xml | 18:22 |
| gene_hacker | http://spie.org/x48084.xml .title | 18:23 |
| kanzure | .title | 18:23 |
| yoleaux | Magnetically responsive photonic nanostructures | 18:23 |
| kanzure | you could probably 3d print a magnetic material | 18:23 |
| gene_hacker | they're pretty | 18:24 |
| gene_hacker | HP is doing that here | 18:24 |
| gene_hacker | with the university | 18:24 |
| gene_hacker | it makes me really angry though, because HP has inkjet dev-kits that can jet photopolymer, but they aren't selling them to anybody | 18:25 |
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| kanzure | hm | 18:26 |
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| kanzure | "We began by developing a robust procedure for preparing magnetite (Fe3O4) colloidal nanocrystal clusters (CNCs) with uniform size (∼30–180nm).3" | 18:31 |
| kanzure | oh, well, magnetite is exactly what the bacteria are producing | 18:31 |
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| kanzure | yashgaroth: bought an ABI 391 | 19:32 |
| yashgaroth | sweet | 19:32 |
| yashgaroth | which one, the $500 from ebay? | 19:32 |
| kanzure | yep | 19:32 |
| kanzure | need arsenal of "primers i wish i would remember to synthesize some day" | 19:33 |
| yashgaroth | I'll have some primer requests if you get it working | 19:33 |
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| kanzure | openscad is having an explosive thread about language design | 19:49 |
| kanzure | it is silly | 19:49 |
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| kanzure | under that "OpenSCAD 3000" title http://rocklinux.net/pipermail/openscad/2014-June/thread.html | 19:52 |
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| kanzure | "The US also rigged the Norwegian selection of the F-35. It was was pretty much pre-decided that Norway had to go for the F35 for political reasons, even though all the committees preferred the Swedish JAS Gripen. The US gave them a great excuse by denying the export by Raytheon of an important upgrade. All this was revealed by Wikileaks." http://www.flightglobal.com/blogs/the-dewline/2010/12/wikileaks-shows-us-played-aesa/ | 20:15 |
| kanzure | oh.. http://en.wikipedia.org/wiki/Lockheed_bribery_scandals | 20:16 |
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| nmz787 | kanzure: so good or bad idea to do an invite on meetup.com for the synthesizer take-apart? | 20:37 |
| kanzure | i think you should do it alone first | 20:38 |
| kanzure | i really wouldn't trust random people to know how to do a sane teardown (where it gets put back together) | 20:39 |
| kanzure | or how to sanely take pics of every component; people get bitchy when you're trying to be comprehensive like that | 20:39 |
| nmz787 | well it could be a 'watch me work' thing | 20:43 |
| nmz787 | idk | 20:43 |
| nmz787 | i'm fine with lonerism | 20:43 |
| nmz787 | this was cool http://zeptobars.ru/en/read/FTDI-FT232RL-real-vs-fake-supereal | 20:58 |
| nmz787 | this was what another guy used, who presented at bsides http://www.amazon.com/DAddario-VR200-Natural-Rosin-Light/dp/B0002OP0WC/ | 20:58 |
| nmz787 | $3 | 20:58 |
| nmz787 | apparently people haven't done, but some ppl are interested in, decompiling an FPGA binary blob | 20:59 |
| kanzure | between jblake, azonenberg and iimarckus i think at least one of them have done that twice and also forgotten about it | 21:00 |
| gradstudentbot | Hood life: https://www.facebook.com/photo.php?fbid=296519020495014 | 21:02 |
| nmz787 | back to verilog? | 21:04 |
| nmz787 | like, here's a binary blob, we know it was on a spartan 3a, get me the verilog | 21:04 |
| nmz787 | damnit | 21:08 |
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| nmz787 | now i'm reading about electro-phoresis/osmosis/kinetic flow | 21:08 |
| nmz787 | it's good, but I didn't plan to get sucked into this | 21:08 |
| kanzure | solidworks steam locomotive http://ckhollidayplans.com/build.html | 21:21 |
| nmz787 | i really want to see some stuff on a thorium steam powered car | 21:25 |
| nmz787 | or VTOL | 21:25 |
| nmz787 | idk maybe steam is not ideal since you need to fill up on H2O | 21:26 |
| nmz787 | but it seems resistant to solar flares and EMP | 21:26 |
| nmz787 | which in the face of the sun we're pretty screwed | 21:26 |
| nmz787 | like, oh shit, those people who print all their PDFs aren't screwed | 21:27 |
| gradstudentbot | Sorry for wasting your time. | 21:29 |
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| gene_hacker | whoa there cowboy, thorium reactors require fast neutrons and generate lots of gamma rays | 21:41 |
| gene_hacker | if you could build a thorium powered car, you would not want to be near it | 21:42 |
| gene_hacker | it would be really cool to see at night though | 21:42 |
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| nmz787 | wouldn't there be enough power to haul around the required shielding? | 21:52 |
| kanzure | .g phosphoramidite supplier | 21:55 |
| yoleaux | http://www.bioneer.co.kr/products/Oligo/DNARNASynthesisReagents-overview.aspx | 21:55 |
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| gene_hacker | well why bother when you can make it unmanned? | 21:56 |
| gene_hacker | who needs underglow when you have cherenkov radiation? http://i41.photobucket.com/albums/e256/blinddemonz/My%20Car/Underglow/underglow2.jpg | 21:56 |
| kanzure | "Direct sunlight should also be avoided during operation of your PE/Applied Biosystems instru | 21:57 |
| kanzure | "Methylimidazole/Tetrahydrofuran | 21:58 |
| kanzure | "Methylimidazole/Tetrahydrofuran lifetime on instrument: 6 weeks" | 21:58 |
| gene_hacker | anyway just what is the plan with the old synth? | 21:58 |
| kanzure | well that's inconvenient | 21:58 |
| gene_hacker | do we have a fume hood for it? | 21:58 |
| kanzure | hmm a fume hood is required: "The waste bottle is the low pressure side of the delivery system and must always be kept vented to atmosphere. Be sure the vent line is properly routed to a fume hood. If the vent line is blocked, back pressure will be generated which will decrease the deliveries of reagents and solvents." | 22:01 |
| gene_hacker | yup | 22:01 |
| kanzure | "The vent line carries gaseous waste to a suitable exhaust such as a fume hood. Applied Biosystems supplies a 25-foot length of tubing to connect the vent line to a fume hood. Proper venting allows operation of the Model 391 on an open lab bench." | 22:01 |
| kanzure | "Prevent condensation from collecting in the vent line by continuously sloping the tubing upward toward the fume hood." | 22:02 |
| kanzure | ammonium hydroxide, acetonitrile (4 L), anhydrous acetonitrile (not HPLC acetonitrile), toluenesulfonic acid, argon cylinder size 1A (regulator and adaptor), phosphoramidites bottled as powders sealed under argon pressure (stable for 1 year minimum) (dissolved in the anhydrous acetonitrile), deoxyinosine, tetrazole/acetonitrile (180 mL), acetic anhydric/lutidine/THF (180 mL), 1-methylimidazole (180 mL), trichloroacetic acid (450 mL), ... | 22:12 |
| kanzure | ... iodine/water/pyridine/THF (200 mL) | 22:12 |
| kanzure | "The synthesizer generates 1 to 2 liters of hazardous, halogenated, organic liquid waste per 100 base additions. The waste is collected in a 4 liter polyethylene bottle which is placed on the floor or on a nearby bench LOWER than the instrument. The bottle can be kept inside a protective carrier to contain accidental spillage. A one-gallon carrier is sufficient and can be purchased from VWR, Part Number 56609-186; or Nalge, Part Number ... | 22:15 |
| kanzure | ... 6501-0010. When the bottle is full, it must be emptied as follows: unscrew the cap assembly and immediately recap the bottle to prevent the escape of vapros. Save and ruse old bottle caps. Discard the waste. Place the liquid in a sealed container labeled "FLAMMABLE", "POISON B N.O.S." or absorb in vermiculite, dry sand or earth. Dispose of the waste following applicable government regulations. Wear gloves and eye protection. Avoid ... | 22:15 |
| kanzure | ... inhalation and skin contact." | 22:15 |
| kanzure | lolz 2 liters "hazardous, halogenated, organic liquid" waste per 100 bp | 22:15 |
| kanzure | hahaha | 22:15 |
| gene_hacker | that's a surprising amount | 22:25 |
| gene_hacker | which means if you want to synthesize big stuff you're gonna generate a lot of wase | 22:26 |
| kanzure | 98% coupling efficiency, can't generate things too long without it screwing up | 22:27 |
| kanzure | here's the docs http://diyhpl.us/~bryan/papers2/DNA/abi391/ | 22:27 |
| kanzure | they claim up to 175 bp | 22:28 |
| justanotheruser | kanzure: how many research papers do you read per day? | 22:29 |
| kanzure | over 9000 | 22:29 |
| justanotheruser | o | 22:30 |
| justanotheruser | 30 (estimate) * 365 * 9000 ~= 100 million | 22:30 |
| nmz787 | justanotheruser: 30 hours per kanzure's day? | 22:31 |
| justanotheruser | Finally, Arif Jinha at the University of Ottawa has recently estimated that the number of journal articles published since time began is about 50 million [3] | 22:31 |
| nmz787 | kanzure: the waste is why I want microfluidics! | 22:32 |
| justanotheruser | nmz787: no, 30 years per kanzures life | 22:32 |
| nmz787 | kanzure: and also that we have to pay for the pre-waste | 22:32 |
| nmz787 | oh | 22:32 |
| kanzure | i am approximately 24.5 years old at the moment | 22:32 |
| justanotheruser | O_o | 22:32 |
| kanzure | nine thousand https://www.youtube.com/watch?v=TBtpyeLxVkI | 22:33 |
| justanotheruser | kanzure: <3 Pokemon | 22:33 |
| nmz787 | a gallon of waste can be between like $50 and $250 to get rid of | 22:33 |
| kanzure | they claim it's okay to dump it into soil | 22:34 |
| justanotheruser | nmz787: BS | 22:34 |
| kanzure | SO IT MUST BE TRUE | 22:34 |
| kanzure | just don't let the goats eat it or something | 22:34 |
| justanotheruser | nmz787: It doesn't cost more than $1 in gas to drive to a nearby body of water for me. | 22:34 |
| kanzure | not sure if dumping organic synthesis waste into freshwater is smart | 22:35 |
| nmz787 | that's how portland became a superfund site | 22:35 |
| justanotheruser | kanzure: It's okay, it's salt water | 22:35 |
| justanotheruser | Well it has become salt water since I've been dumping so many salts into it ^_^ | 22:36 |
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| kanzure | i guess 30 isn't that different from 24 | 22:37 |
| justanotheruser | kanzure: sry | 22:37 |
| kanzure | shrug, half the people in here think i'm sort of brain damaged self-hating professor working in an academic lab | 22:38 |
| justanotheruser | kanzure: do you believe in IQ tests? | 22:38 |
| kanzure | i believe there are tests called iq tests | 22:38 |
| justanotheruser | do you believe in their merits? | 22:39 |
| kanzure | very few of the merits, although some of them | 22:39 |
| justanotheruser | have you taken one? | 22:39 |
| kanzure | yeah, a few, i took more when i was younger, and less as i grew older, and the ones i was taking less were also less uh.. good. the earlier ones were much more rigorous. | 22:40 |
| justanotheruser | what did you get? | 22:40 |
| kanzure | i've consistently scored 120ish | 22:41 |
| justanotheruser | hm | 22:41 |
| nmz787 | kanzure: last time i heard i thought you weren't even 21 yet | 22:41 |
| kanzure | well you've known me for a while | 22:41 |
| kanzure | we met in 2009 | 22:41 |
| justanotheruser | nmz787: well if you average my guess and your guess, we get close to his age | 22:41 |
| nmz787 | justanotheruser: i thought you were saying 30 year life expectancy from now | 22:42 |
| kanzure | honestly i'm surprised i'm not closer to 100 | 22:42 |
| justanotheruser | nmz787: no | 22:42 |
| nmz787 | he took a few years to learn to read | 22:42 |
| justanotheruser | I thought he was 30 based on his SKDB talk video :P | 22:42 |
| kanzure | there were two people in that one, btw | 22:43 |
| justanotheruser | are you the one with glasses and sideburns? | 22:43 |
| kanzure | probably | 22:43 |
| kanzure | i am not the short scruffy one | 22:43 |
| justanotheruser | Because you look about 20-25 in that video and it was in 2009 | 22:44 |
| kanzure | if you mean the guy that looks like aubrey, that's todd huffman | 22:44 |
| justanotheruser | the guy who basically talks the whole time in this and is introduced as bryan bishop http://www.youtube.com/watch?v=-n39RK4inzg&list=PL9F4F7BF006E610E6 | 22:44 |
| kanzure | yes the guy who is introduced as me is e | 22:45 |
| kanzure | *is me | 22:45 |
| kanzure | regarding iq, i don't think intelligence is a very good explanation of what the brain is doing | 22:45 |
| kanzure | or description for that matter | 22:45 |
| justanotheruser | I think so too | 22:45 |
| justanotheruser | I think you have to have a certain intelligence to be able to do it | 22:46 |
| kanzure | to do what? | 22:46 |
| justanotheruser | kanzure: teach self diybio | 22:46 |
| justanotheruser | and read and understand bio research papers | 22:46 |
| justanotheruser | I guess probably >100 | 22:47 |
| justanotheruser | can do it | 22:47 |
| justanotheruser | then you have to be persistent | 22:47 |
| kanzure | most papers are crap | 22:48 |
| gradstudentbot | I'm writing that up and it will be submitted soon. | 22:48 |
| kanzure | gradstudentbot: can i coauthor that paper? | 22:48 |
| gradstudentbot | I coauthored a paper about that a few years ago. | 22:48 |
| nmz787 | ttyl | 22:54 |
| kanzure | and papers are sorta silly anyway | 22:59 |
| archels | nmz787: what was the URL for that online ESD safety training? | 22:59 |
| kanzure | justanotheruser: btw that wasn't a paper; it's the manual to a toy i bought | 23:00 |
| kanzure | http://diyhpl.us/~bryan/papers2/DNA/abi391/ABI%20391-manual.pdf | 23:01 |
| archels | it synthesises DNA *and* brews yerba mate? that's brilliant | 23:03 |
| kanzure | it also generates a lovely amount of "industrial byproduct" | 23:04 |
| archels | how do you validate the output product? Do you also have (access to) a sequencer? | 23:05 |
| kanzure | spectrophotometer | 23:05 |
| kanzure | old school way is gfp, antibiotic resistance, toxin resistance, etc | 23:06 |
| archels | right | 23:06 |
| kanzure | wouldn't mind having a dna sequencer but it's not high on my priority list | 23:06 |
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| kanzure | "It is important to keep the oligonucleotides cold to minimize bacterial growth." | 23:19 |
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| kanzure | appendix B page 4 has a schematic of the fluidic circuit | 23:23 |
| nmz787 | archels: internal to company | 23:24 |
| nmz787 | g'night | 23:24 |
| kanzure | "Temperatures below 16 C (60 F) must be avoided because they will cause the tetrazole to precipitate from solution." | 23:32 |
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| kanzure | "A regulated cylinder (size 1A) of prepurified (99.998%) argon must be connected to the rear panel of the synthesizer. The instrument has a gas input that accepts 1/4-in.-o.d. ParflexTM tubing with appropriate Swagelok® tube fittings (1/4-in. nut and ferrule). A 10-ft length of ParflexTM tubing (1/4-in. o.d.) and the necessary Swagelok® fittings to connect this tubing to the instrument are provided." | 23:33 |
| kanzure | "The argon tank requires a two-gauge regulator with a CGA (Compressed Gas Association) 580 argon cylinder adapter on the inlet side and a Swagelok® fitting that accepts a 1/4-in.-o.d. tube. The primary gauge (0-3000 psi; 0-25,000 kPa recommended) measures tank pressure, and the secondary gauge (0-200 psi; 0-2000 kPa recommended) measures regulated pressure. The regulator must be obtained by the user prior to installation." | 23:33 |
| kanzure | "Typical argon consumption is approximately 3.0 m3 every four weeks. Size 1A cylinders have a capacity of 8.2 cm3 and should last approximately three months. The tank should be regulated to approximately 65 psi (450 kPa)." | 23:34 |
| kanzure | er, 3 m^3 | 23:34 |
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| kanzure | argon cylinder size 1A is 75 kg | 23:40 |
| --- Log closed Wed Jun 18 00:00:38 2014 | ||
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