2014-06-23.log

--- Log opened Mon Jun 23 00:00:43 2014
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archelshttp://matplotlib.org/users/whats_new.html#xkcd-style-sketch-plotting05:57
archelstomfoolery05:57
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kanzure.title https://www.youtube.com/watch?v=eXXmUWD9_0w06:16
yoleauxMt Lofty Television Transmitter Station: A Brief Tour06:16
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chris_99ahhh paperbot where art thou06:54
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eudoxiai like to imagine a big bot in a hospital bed with a sad emoji on a screen06:56
eudoxia🙀06:57
eudoxiathat's U+1F640 btw06:57
chris_99hehe06:57
chris_99poor paperbot06:58
kanzurehttp://img.photobucket.com/albums/v314/streamwolf8/Anime%20North%202008/DSCF0860.jpg06:58
kanzurehttp://fc06.deviantart.net/fs71/f/2013/148/1/0/flcl__canti_by_deckronomicon-d66zl5u.jpg06:59
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kanzureParahSailin: what was your comment about antivenom about?08:18
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ParahSailini was surprised to find out that antivenom is typically just polyclonal antibody extracted as serum08:19
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kanzurei think in cases of scorpion antivenom they are literally just raising scorpions on farms and extracting fluids regularly08:21
kanzurewasn't there something about an antivenom shortage or something08:21
kanzure"Why is there shortage of coral snake antivenom? Pfizer/Wyeth has been the sole producer of antivenom in the United States. They no longer are manufacturing it. When present stocks are gone there, will be no FDA-approved antidote for coral snake bites."08:22
ParahSailinthere was, in australia or something a few years ago08:22
kanzureoh brother "Before antivenin was developed in 1967, the fatality rate was reported as high as 10-20%."08:23
kanzure"What’s being done about the antivenom shortage?" "Managing and distributing the remaining Antivenin (Micrurus fulvius)®" "Wyeth restarted the manufacturing process, and is planning to reintroduce Antivenin (Micrurus Fulvius) ®. It is unknown how soon a new supply will be available."08:23
cluckj1967?08:23
ParahSailinkanzure: yeah, if you have polyclonal serum production system, you need regular supply of mammals and antigen08:24
cluckjthat's a little bit late iirc08:24
kanzure"Each vial of CroFab costs a hospital about $2,000" haha08:24
cluckjmaybe poisonous snakes could just be eradicated08:25
cluckjWAR ON VENOM08:25
cluckjSHOCK AND AWE08:25
kanzureor you can just introduce the antibody in advance08:25
cluckjhttp://clinicaltrials.gov/ct2/show/NCT0208132608:29
kanzure.title08:29
yoleauxRepeat BCG Vaccinations for the Treatment of Established Type 1 Diabetes08:29
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cluckjI think that's my FDA hate for the next few weeks08:30
cluckjstudy completion: 202208:31
kanzure"Antivenin is the proper term for animal derived serums which are more like vaccines than traditional chemically manufactured medicines. Antivenom is the general term for anything else derived through non-biological substances."08:31
kanzurehmmm08:31
cluckjmeanwhile billions of people get that safe and effective vaccine08:32
cluckjmaybe I can convince my doctor to give it to me08:32
kanzuredo it yourself08:32
cluckjyeah08:33
cluckjthe county department of health probably has that vaccine08:33
kanzureantivenin/antivenom is a highly unfortunate distinction08:35
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heathanother reason not to use the ybit nick10:36
heath10:35 < sinious> >> var areYouMyBitch = true; console.log(areYouMyBitch + '!');10:36
heath^ that highlights10:36
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heathfind .11:11
heathdidn't realize that acted like tree11:11
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eudoxiai came up with a new line for gradstudentbot11:17
eudoxia"The electron microscope burned my sample"11:17
eudoxiaahahahahahahhahha11:18
kanzuredid this happen to you?11:19
eudoxiano11:21
eudoxiadoes that mean it's disqualified?11:21
kanzureno11:21
eudoxiaanyways i read in Richard Jones' heretical anti-MNT book that it's a thing that happens11:25
eudoxiahis PhD was originally in how globular proteins move in fluids and the TEM kept burning his samples :V11:26
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||0_-_0||just show me the pictures11:27
||0_-_0||paperbot http://www.sciencemag.org/content/344/6190/137311:27
paperbothttp://libgen.org/scimag/get.php?doi=10.1126%2Fscience.125229111:27
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dingoi wrote my own chat server, telnet 1984.ws 602312:25
dingoits an all-in-one irssi-like clone12:26
dingohehe12:26
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kanzureThe black thing inside rejoices at your departure.12:30
dingo:-)12:30
dingoi got that from an old dos game, "legend of the reg dragon"12:31
kanzureParahSailin: would you be happy with just doing the 1918 influenza virus, or does it have to be a modern supervirus?12:31
ParahSailindo what?12:31
kanzurefor your freedom virus12:32
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ParahSailinnot any extra trouble to do modern supervirus i suppose12:34
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kanzure100k bp per $112:46
kanzureoh.. the inkjet dna synthesizer that church is referring to is actually posam/pogo http://bioinformatics.org/pogo/12:47
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kanzurei wish they would just say that. it was burried 5 references deep.12:47
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kanzuregl45 cap connection system http://www.duran-group.com/uploads/pics/uebersicht_ASS-GL45sm_05.jpg13:23
kanzurethey spent $23,000 on their motion control and actuation stuff? http://bioinformatics.org/pogo/POSAM_Man_Ch1_Assembly_v1-2_040601.pdf13:26
ParahSailinthe inkjet microarray printing is pretty much obsoleted by photolithography microarray printing13:28
kanzurebut with inkjetting you don't need photolabile protecting groups or photogenerated acids13:42
kanzureand the droplets are separate microreactors13:42
ParahSailini will have to do literature review to figure out why photolithography made the inkjet method obsolete13:43
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ParahSailinhm, it might be just as simple as photolithography allowed higher spot density, which is not much of a plus in this case14:01
ParahSailinthere is nothing on posam after 2004?14:06
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kanzureParahSailin: these guys used it for a thing published 2010, http://dukespace.lib.duke.edu/dspace/bitstream/handle/10161/4000/274747200023.pdf%3Fsequence%3D114:45
ParahSailini recognize some common names with the quan paper14:46
kanzuresame group that referenced it, this time 2011 inkjet14:46
kanzureoops14:46
kanzurethis time 2011 http://diyhpl.us/~bryan/papers2/DNA/Parallel%20on-chip%20gene%20synthesis%20and%20application%20to%20optimization%20of%20protein%20expression.pdf14:46
ParahSailinyeah the quan paper14:46
kanzuredouble oops, yes14:47
kanzurephotolithography is a plus if you're already doing photolithography anyway14:48
kanzureanother reason might be that everyone was butthurt by affymetrix's weirdo photomask-based photolithography system14:48
kanzurewhere 25 bp sequences required 100 masks14:49
kanzureerm, i don't know which direction that reason was supposed to be suporting14:49
kanzure*supporting14:50
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kanzureone of the gen9bio people does art for http://collisioncollective.org/content/about-collisioncollective ("a group show dealing with two closely-related concepts: human self-modification and the human modification of our environment")15:15
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eudoxiakanzure: the other day you said other h+ communities made us look like demigods15:17
kanzuredefinitely15:18
eudoxiawhat would be the ten Herculean Labors?15:18
eudoxiaof transhumanism15:18
kanzurei don't know, you figure it out15:18
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kanzurethe point is, writing magazine articles doesn't magically make LSD15:19
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||0_-_0||kanzure Demigod of Grumpywit15:26
kanzurei said *seem* like demigods,15:26
kanzureany advanced.. sufficiently magic.. etc.15:26
||0_-_0||Grumpywit and Literalism15:27
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dbolserI think I'm h-15:49
gnusha_https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f88d4b09 Bryan Bishop: elaborate opencascade methods >> http://diyhpl.us/diyhpluswiki/cad/opencascade/15:57
gnusha_https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=4382edcd Bryan Bishop: some extruded aluminum scaffolding >> http://diyhpl.us/diyhpluswiki/scaffolding/15:57
kanzure"We use uv-cureable polymers and printed masks for the mold, PDMS & SIFEL (fluorinated silicon) for the chip, vakuum pumps to degas the PDMS  and corona treatment to bond the PDMS to glass."16:04
gnusha_https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=6d6a42c7 Bryan Bishop: gah, i forgot about gridbeam >> http://diyhpl.us/diyhpluswiki/scaffolding/16:06
gnusha_https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f480398b Bryan Bishop: ah, defer to reprap for scaffolding >> http://diyhpl.us/diyhpluswiki/scaffolding/16:10
kanzure"Parametrically Designed XY Motion Stage" http://web.mit.edu/imoyer/www/portfolio/parametric/16:14
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kanzure.wik asco numatics18:01
yoleaux"The Emerson Electric Company (NYSE: EMR) is an American multinational corporation headquartered in Ferguson, Missouri, United States. This Fortune 500 company manufactures products and provides engineering services for a wide range of industrial, commercial, and consumer markets." — http://en.wikipedia.org/wiki/ASCO_Numatics18:01
kanzureoh hm.18:02
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kanzurecadquery is pretty fun when used inside freecad20:55
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ebowdenImagine scritching the ears when they take they hat off after a long day.21:15
ebowdenOoh, wrong window.21:16
ebowden:D21:16
kanzurevery wrong21:18
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kanzurei think i'll convert mcad.git into cadquery21:46
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gene_hackerPDMS swells with most solvents used in DNA synthesis22:48
gene_hackerbut with inkjetting, you need a special inkjet that can handle weird stuff22:49
nmz787_iyup22:50
nmz787_ior fluridated polymers22:50
nmz787_ifluoridated*22:50
gene_hackerand those special inkjets could be really cheap too if it wasn't for that darned HP22:51
gene_hackeror other inkjet manufacturers who aren't willing to sell inkjet devkits for fear of people making their own replacement inks22:53
gene_hackerthose kilobaser people do have an interesting method of doing corona treatment though22:55
gene_hackerthey use a microwave oven22:55
nmz787_ithat's how they usually work22:55
nmz787_ithe lab-branded ones just have tunable freq22:55
nmz787_ialso there's a video on youtube from a few years ago showing the whole setup22:56
nmz787_ithose guys never emailed me back anyway22:56
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gene_hackerwell if they can get 1 kilobase with low enough errors, things could get interesting22:58
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--- Log closed Tue Jun 24 00:00:44 2014

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