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| mosasaur | "Dr. Kumar will describe the challenges in developing small, agile robots ..." Someone's crossing the development streams here. | 04:58 |
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| cluckj | you're not supposed to cross the streams :( | 05:20 |
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| nickjohnson | £300 left to the goal on my KS, the tension is killing me. | 05:36 |
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| CaptHindsight | nickjohnson: silent corduroy for military? link? | 06:04 |
| nickjohnson | CaptHindsight: whaaat? | 06:05 |
| CaptHindsight | just wondering what your project is | 06:05 |
| chris_99 | didn't someone actually invent silent velcro heh | 06:06 |
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| nickjohnson | CaptHindsight: http://kck.st/1GdYIVu | 06:12 |
| * mosasaur wonders if this device can be somehow be used to sync RTLSDR dongles | 06:18 | |
| superkuh | As much as any clock generator, but there'll still be the problems with no meta-data and USB latency and weirdness. | 06:25 |
| chris_99 | i found a new use for my electrophoresis psu, powering my nixies | 06:26 |
| superkuh | Oh, wait, no. You'd need to multiply it. You need a 28.8 MHz signal for the rtlsdr. | 06:26 |
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| kanzure | paperbot: http://pubs.acs.org/doi/abs/10.1021/acsnano.5b01060 | 06:55 |
| kanzure | .title | 06:55 |
| kanzure | hmph | 06:55 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/Neural%20Stimulation%20and%20Recording%20with%20Bidirectional%2C%20Soft%20Carbon%20Nanotube%20Fiber%20Microelectrodes.txt | 06:55 |
| yoleaux | kanzure: Sorry, that command (.title) took too long to process. | 06:55 |
| kanzure | "Neural Stimulation and Recording with Bidirectional, Soft Carbon Nanotube Fiber Microelectrodes" | 06:56 |
| kanzure | "The development of microelectrodes capable of safely stimulating and recording neural activity is a critical step in the design of many prosthetic devices, brain machine interfaces and therapies for neurologic or nervous-system-mediated disorders. Metal electrodes are inadequate prospects for the miniaturization needed to attain neuronal-scale stimulation and recording because of their poor electrochemical properties, high stiffness and ... | 06:56 |
| kanzure | ... propensity to fail due to bending fatigue. Here we demonstrate neural recording and stimulation using carbon nanotube (CNT) fiber electrodes. In vitro characterization shows that the tissue contact impedance of CNT fibers is remarkably lower than state-of-the-art metal electrodes, making them suitable for recording single neuron activity without additional surface treatments. In vivo chronic studies in parkinsonian rodents show that ... | 06:56 |
| kanzure | ... CNT fiber microelectrodes stimulate neurons as effectively as metal electrodes with ten times larger surface area, while eliciting a significantly reduced inflammatory response. The same CNT fiber microelectrodes can record neural activity for weeks, paving the way for the development of novel multifunctional, dynamic neural interfaces with long-term stability." | 06:56 |
| kanzure | "In collaboration with the Pasquali lab, we have developed a fabrication method for stereotrodes made from 40 um diameter CNTfs which are coated in an insulating elastomer (2 um layer), leaving only the tips exposed. In vitro and in vivo have been conducted to characterize the electrochemical stability of the electrodes. Over a week of chronic current pulsing in phosphate buffered saline (PBS), we have shown with impedance spectroscopy ... | 06:57 |
| kanzure | ... and cyclic voltammetry that the CNTfs are stable. They are also shown to have a larger charge storage capactiy and water window relative to platinum iridium (PtIr) stereotrodes of similar size. These stereotrodes have been implanted in the GPi of a cohort of rats (n = 4) for 6 weeks and voltage transients were recorded regularly over this time period to confirm in vivo stability." | 06:57 |
| kanzure | "On the internet, nobody understands that nobody else understands 'nobody cares if you are a dog'." | 07:25 |
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| kanzure | http://www.black-swift.com/ "a tiny — almost coin-sized — computer with 32-bit CPU, megabytes of memory, integrated 802.11 b/g/n Wi-Fi, USB 2.0, and other interfaces" | 08:05 |
| kanzure | "25 GPIO lines, hardware SPI and UART, software I2C, 1-wire, and PWM support. Two Ethernet interfaces, an USB 2.0 port and 802.11 b/g/n Wi-Fi with onboard antenna" | 08:06 |
| chris_99 | $26!!! | 08:08 |
| chris_99 | do they think we're made of money ;) | 08:08 |
| cluckj | aww it's so cute | 08:09 |
| CaptHindsight | Qualcomm Atheros AR9331 SoC | 08:15 |
| CaptHindsight | I was looking over the microfluidic dna sythesizers ...http://www.nature.com/nrd/journal/v11/n8/images/nrd3799-f2.jpg | 08:19 |
| CaptHindsight | how useful is something with so few chambers? | 08:20 |
| CaptHindsight | or is this interesting since it's a single chip design? | 08:21 |
| CaptHindsight | don't you want each chamber to have individual temp control | 08:24 |
| kanzure | something with that many valves is much harder to debug | 08:30 |
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| kanzure | i think a continuous flow design would be much simpler to implement | 08:31 |
| kanzure | most of the macro-sized dna synthesizers have <16 columns anyway. | 08:31 |
| kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/synthesis/ | 08:32 |
| kanzure | see figure 1a for another design (trace the input/output flow carefully) http://diyhpl.us/~bryan/papers2/microfluidics/synthesis/Synthesis%20-%20Microfluidic%20PicoArray%20synthesis%20of%20oligodeoxynucleotides%20and%20simultaneous%20assembling%20of%20multiple%20DNA%20sequences%20(10%20kb).pdf | 08:34 |
| CaptHindsight | also depends on how much you want to make, we were making 2L at a time | 08:35 |
| CaptHindsight | the next design was for 1K Liter batches | 08:36 |
| kanzure | couldn't you just use pcr | 08:38 |
| CaptHindsight | these PicoArrays are for uL sizes | 08:38 |
| kanzure | once you synthesize the strands, you cna just copy them- you don't need to synthesize multiple kilograms | 08:39 |
| kanzure | *can | 08:39 |
| CaptHindsight | yeah | 08:39 |
| CaptHindsight | seed and copy | 08:39 |
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| CaptHindsight | I'm just looking at designing for mass production and automation | 08:40 |
| kanzure | in microfluidic designs the chambers are usually for synthesizing different strands simultaneously in parallel | 08:40 |
| kanzure | and then you later combine them | 08:40 |
| CaptHindsight | automating that combining part would speed things up | 08:41 |
| kanzure | afaik nobody has actually done a 1024x1024 pixel (or greater) microfluidic dna synthesizer anyway | 08:47 |
| kanzure | so just making such a device in the first place would be a step up | 08:47 |
| kanzure | make some 50 bp strands in each pixel/well/location, combine. sequence after each combination, throw away anything wrong. etc. | 08:48 |
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| CaptHindsight | http://diyhpl.us/~bryan/papers2/Maskless%20fabrication%20of%20light-directed%20oligonucleotide%20microarrays%20using%20a%20digital%20micromirror%20array.pdf | 09:09 |
| kanzure | also http://diyhpl.us/~bryan/papers2/optics/photolithography/ | 09:10 |
| CaptHindsight | 600 x 800 using a dlp | 09:10 |
| kanzure | i'm sure we could do better these days | 09:10 |
| CaptHindsight | 1920 x 1080 | 09:10 |
| kanzure | but yeah even 600x800 is useful | 09:10 |
| CaptHindsight | or epson has 1920 x ~1200 | 09:11 |
| CaptHindsight | I've been making arrays with them for SLA | 09:11 |
| CaptHindsight | whats the reaction time for light-directed oligonucleotide synthesis? | 09:13 |
| kanzure | i believe it's minutes per reaction step | 09:13 |
| CaptHindsight | thanks, was scanning through the docs | 09:13 |
| kanzure | <20 minutes per step | 09:14 |
| kanzure | there's a non-light synthesizer that has lots of public documentation, called uh, pogam? | 09:14 |
| kanzure | http://bioinformatics.org/pogo/ | 09:14 |
| kanzure | see "User manual chapter 2: Operation" http://bioinformatics.org/pogo/POSAM_Man_Ch2_User_v1-0_040414.pdf | 09:14 |
| CaptHindsight | I work on this all the time just not for DNA synthesis | 09:14 |
| kanzure | *posam | 09:15 |
| kanzure | posam not pogam | 09:15 |
| CaptHindsight | yeah 1920(H)×1200(V) | 09:16 |
| CaptHindsight | Pixel pitch 8.5(H)×8.5(V)um without any magnification or reduction | 09:16 |
| CaptHindsight | this could be a $1000 device | 09:17 |
| kanzure | easily. yep. | 09:18 |
| CaptHindsight | http://www.bioinformatics.org/pogo/ | 09:19 |
| kanzure | i just linked you to that :P | 09:19 |
| CaptHindsight | yeah jut looking at it | 09:20 |
| CaptHindsight | want to see which heads they used | 09:20 |
| CaptHindsight | EpsonTM F057020 print head | 09:21 |
| kanzure | iirc it was something that nobody is making anymore | 09:21 |
| kanzure | more details may be in the logs. i think we identifed a compatible alternative? | 09:21 |
| CaptHindsight | I work with ~100 different printheads | 09:22 |
| kanzure | brutal | 09:22 |
| CaptHindsight | the epsons are easily and cheaply found in printers | 09:23 |
| CaptHindsight | it's just that they are the aqueous version heads | 09:23 |
| CaptHindsight | http://global.kyocera.com/prdct/printing-devices/inkjet-printheads/ | 09:25 |
| CaptHindsight | if you want lots of small drops rapidly | 09:26 |
| CaptHindsight | what routes to market (or end users) have people taken when they have developed new types of medical equipment? | 09:31 |
| kanzure | lots of fda approval stuff :\ | 09:32 |
| kanzure | personally my recommendation is to go the kit route for now | 09:32 |
| kanzure | .wa number of nucleases per m^3 of exhaled human breath | 09:34 |
| yoleaux | kanzure: Sorry, no result! | 09:34 |
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| kanzure | "who stalks the stalkers?" | 10:34 |
| cluckj | anthropologists | 10:36 |
| mosasaur | amazon | 10:48 |
| cluckj | facebook? | 10:49 |
| kanzure | cluckj: ah but who stalks the anthropologists? an even larger, meatier anthropologist? or do they all just anthropologize each other a few times a week as a sort of time-sharing deal? | 10:51 |
| cluckj | anthropologists are incredibly tedious to study | 10:52 |
| cluckj | I guess it's the last of those | 10:53 |
| cluckj | speaking of super tedious, I need to go write | 11:02 |
| cluckj | and come up with some nice questions about paperbot, maybe | 11:02 |
| * cluckj pets paperbot | 11:02 | |
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| nmz787_i | paper-mache-bot GO! | 11:06 |
| * nmz787_i 110 pg schemtic to validate manually, whee! | 11:07 | |
| kanzure | .title https://people.xiph.org/~xiphmont/demo/daala/random.shtml | 11:25 |
| yoleaux | next generation video [interlude]: Daala blog-like post 20150325 | 11:25 |
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| kanzure | 11:43 <@gwern> Burninate: my reddit account got subpoenaed by ICE; I mentioned this yesterday | 11:48 |
| kanzure | 11:45 <@gwern> Burninate: some reddit dramaz over supposed doxing/leaks of the Evolution market admins. I can't afford a lawyer, and it'd be a waste of money since it's an administrative subpoena - they can't be fought | 11:48 |
| kanzure | 11:47 < Burninate> Are you exposed in any capacity by your Reddit account? | 11:48 |
| kanzure | 11:48 <@gwern> Burninate: oh sure. my IP is all over it | 11:48 |
| kanzure | http://pastebin.com/m9RGY9NH | 11:53 |
| ParahSailin | does everyone get a different partial preview in google books? | 12:05 |
| kanzure | yes i think so | 12:14 |
| ParahSailin | nobody has made a botnet to eat books from there yet? | 12:14 |
| archels | nickjohnson: don't be silly, that last £300 you'd just put up yourself | 12:16 |
| archels | or £153 by this point---nothing to worry about by the looks of it | 12:17 |
| nickjohnson | archels: Yeah, nothing to worry about, I just want to hit the damn goal already :P | 12:18 |
| archels | greedy! | 12:18 |
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| nmz787_i | I want an amino acid sampler pack... like each amino alone, such that I could taste them all... seems like it could be a good science-bar gimmick too... serve 'flights' of amino + vodka or something | 12:31 |
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| andytoshi | that is not necessarily safe ... used to be tryptophan was sold as a dietary supplement but fda stopped it for some reason or another | 12:39 |
| nmz787_i | trippin on trypto | 12:39 |
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| nmz787_i | nah, i don't think that'd be a problem, tons of tryptophan in milk | 12:39 |
| nmz787_i | anymore than overdosing on other common foods | 12:40 |
| andytoshi | there is in fact a 20-step process of synthethizing lysergic acid starting with only tryptophan and standard reagents | 12:40 |
| andytoshi | that may have something to do with it | 12:41 |
| andytoshi | but they claimed that tryptophan supplements let to increased cancer risk or something | 12:41 |
| nmz787_i | yeah, they cloned part of the operon that the fungus uses | 12:41 |
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| nmz787_i | http://semver.org/ | 12:53 |
| nmz787_i | .title | 12:53 |
| yoleaux | Semantic Versioning 2.0.0 | 12:53 |
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| fenn | black-swift looks like a good quadcopter controller. surprising to see an actual product coming out of moscow | 13:15 |
| fenn | andytoshi: tryptophan is perfectly safe. the fda recall was because of a contaminant related to a manufacturing process that is no longer used | 13:27 |
| andytoshi | fenn: can i buy tryptophan at supplement stores now then? | 13:28 |
| fenn | yes | 13:28 |
| andytoshi | this is good to know | 13:28 |
| fenn | since the 1990s | 13:28 |
| andytoshi | lol | 13:28 |
| andytoshi | that's what i get for believing random shit that gets in my eyes while skimming forums | 13:29 |
| fenn | also, you can't make LSD out of tryptophan, but probably some other psychedelic tryptamines | 13:29 |
| fenn | by "can't" i mean ridiculously complex and unfeasible synthesis | 13:29 |
| fenn | .wik bromocryptine | 13:31 |
| yoleaux | "Bromocriptine (INN; trade names Parlodel, Cycloset, Brotin (Pakistan)), an ergoline derivative, is a dopamine agonist that is used in the treatment of pituitary tumors, Parkinson's disease (PD), hyperprolactinaemia, neuroleptic malignant syndrome, and type 2 diabetes." — http://en.wikipedia.org/wiki/Bromocryptine | 13:31 |
| nmz787_i | .wik gpr | 13:32 |
| yoleaux | "GPR may refer to:" — http://en.wikipedia.org/wiki/Gpr | 13:32 |
| nmz787_i | .wik ground penetrating radar | 13:33 |
| yoleaux | "Ground-penetrating radar (GPR) is a geophysical method that uses radar pulses to image the subsurface. This nondestructive method uses electromagnetic radiation in the microwave band (UHF/VHF frequencies) of the radio spectrum, and detects the reflected signals from subsurface structures." — http://en.wikipedia.org/wiki/Ground_penetrating_radar | 13:33 |
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| nmz787_i | http://www.pcworld.com/article/2902246/new-3d-nand-flash-will-triple-capacity-of-ssds-intel-and-micron-say.html | 14:03 |
| nmz787_i | wow | 14:03 |
| nmz787_i | 437 GB the size of an M&M | 14:03 |
| chris_99 | nice! | 14:07 |
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| kanzure | https://en.bitcoin.it/wiki/User:Gmaxwell/visual_fingerprint_comparison | 14:21 |
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| archels | http://mic.com/articles/113740/a-team-of-biohackers-has-figured-out-how-to-inject-your-eyeballs-with-night-vision | 14:43 |
| kanzure | i thought i was really on to something with smellvision | 14:45 |
| justanotheruser | lol | 14:46 |
| justanotheruser | I like the picture | 14:47 |
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| fenn | an eye dropper would be too pedestrian | 15:26 |
| mosasaur | it still needs a reflective surface behind the retina | 15:27 |
| fenn | chlorin e6 sounds a lot easier than eating a vitamin-A restricted diet for 3 months | 15:28 |
| gnusha | https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8d83cdb7 fenn: chlorin e6 night vision eyedrops >> http://diyhpl.us/diyhpluswiki/senses/ | 15:46 |
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| ParahSailin | what does amazon mean by "unlimited" | 15:58 |
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| nmz787_i | wow that night vision stuff is pretty cool | 16:07 |
| fenn | what was the thing that took a sentence and did a google search for the parts of the sentence and then photoshopped it all together automatically into a somewhat realistic image? | 16:10 |
| fenn | it was called something like sentence2image? | 16:11 |
| fenn | possibly a siggraph paper | 16:11 |
| nmz787_i | that sounds like it would be cool | 16:15 |
| fenn | basically the inverse of karpathy's work | 16:17 |
| kanzure | http://www.google.com/patents/US20120157377 | 16:20 |
| kanzure | is this the "Chlorophyll derivatives as visual pigments for super vision in the red" person | 16:21 |
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| jackybgood | paperbot DOI: 10.1111/j.1095-8312.1984.tb00803.x | 18:11 |
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| kanzure | jackybgood: use a full url | 18:27 |
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| kanzure | MRV: hi | 18:28 |
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| kanzure | hmph | 18:43 |
| kanzure | https://www.quantamagazine.org/20150325-did-neurons-evolve-twice/ | 18:46 |
| kanzure | .to delinquentme nevermind | 18:57 |
| yoleaux | kanzure: I'll pass your message to delinquentme. | 18:57 |
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| kanzure | overtone/supercollider alternatives: http://puredata.info/ https://csound.github.io/ http://chuck.cs.princeton.edu/ https://cycling74.com/products/max/ | 19:36 |
| jackybgood | paperbot http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8312.1984.tb00803.x/abstract | 19:40 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/cb7ed9066d112e87e719abc5666e27b9.txt | 19:41 |
| jackybgood | Well, worth a shot anyway | 19:43 |
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| --- Log closed Fri Mar 27 00:00:34 2015 | ||
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