2015-04-12.log

--- Log opened Sun Apr 12 00:00:50 2015
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Adlaimaaku: it, it, ... them?05:07
Adlai:D05:07
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chris_99Does anyone know of any studies comparing a stir bar vs an orbital shaker for things like growing yeast07:09
yoleaux11 Apr 2015 00:57Z <nmz787> chris_99: ah, cool! what are you gonna use to control the CCD?07:09
chris_99or have any options07:09
chris_99!help07:10
chris_99hmm, how do i leave a message with yoleaux too heh07:10
cluckjI haven't seen stir bars used for growing yeast on a small scale07:11
chris_99oh, they're used a lot in the homebrewing world, but im wondering if a shaker would be the same in performance07:12
cluckjstir bars tend to be attached to hot plates, and orbital shakers tend to also be incubators07:12
cluckjoh07:12
chris_99i've found an orbital shaker with a hotplate07:12
chris_99but i'm not sure if i should get a stir  plate intead07:14
cluckjwhy not both?07:14
chris_99heh, i don't have much spare cash atm07:15
cluckjoh hah07:15
cluckjI think it might be a scale issue....an orbital shaker that can do a 15 gallon drum of beer is gonna be scary as fuck07:16
chris_99oh to make it clear, this is just for a yeast starter07:18
chris_99so only say 1litre or 2litre07:18
chris_99although a 15 gal one would be cooler :)07:21
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cluckjhaha07:28
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Adlaipaperbot: http://pubs.rsc.org/en/Content/ArticleLanding/2013/CS/c3cs35433d08:05
paperbothttp://diyhpl.us/~bryan/papers2/paperbot/New%20insights%20into%20prebiotic%20chemistry%20from%20Stanley%20Miller%27s%20spark%20discharge%20experiments.pdf08:06
Adlaiesgeht!08:07
Adlaipaperbot: http://onlinelibrary.wiley.com/doi/10.1002/anie.198308161/pdf08:14
paperbothttp://diyhpl.us/~bryan/papers2/paperbot/825c1ef0c098892697e4bd594410ed76.txt08:14
kanzurehmph08:14
Adlaihmm.08:14
* Adlai should get to "work" anyway08:15
kanzurebah08:15
kanzureor file bug reports on paperbot08:15
kanzurehttps://github.com/kanzure/paperbot08:15
Adlaiah, but https://github.com/kanzure/paperbot/issues/22 already exists :P08:21
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caternhttps://mchankins.wordpress.com/2013/04/21/still-not-significant-2/08:30
caternlol08:30
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Adlai"For example, “a trend towards significance” expresses non-significance as some sort of motion towards significance, which it isn’t: there is no ‘trend’, in any direction, and nowhere for the trend to be ‘towards’"08:32
CheckDavidWhat do you guys think of fasting?08:37
Adlaileeches have also been said to cure numerous ailments08:38
kanzureCheckDavid: also consider tapeworms08:40
CheckDavidYou drawing an analogy between leeches and fasting Adlai ?08:41
Adlaiyes. are you thinking of fasting? the answer to "why or why not" either makes my analogy relevant or irrelevant... fasting "done right" probably has more psychological benefit than leeches08:43
FourFireCheckDavid, Honey as disinfectant08:43
CheckDavidI did already a 2 day fasting08:43
CheckDavidOh heard that one before FourFire08:43
FourFireDo you understand the mechanism behind it?08:43
CheckDavidFourFire: fasting?08:44
CheckDavidNo I am not aware of the mechanics.08:44
kanzurewhoops i mean tapeworm eggs08:44
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CheckDavidDo you FourFire ?08:45
kanzurei don't even know if non-egged tapeworms work08:45
CheckDavidThey work very hard man.08:45
FourFireCheckDavid, no honey08:47
CheckDavidI read some shit online about fasting and was very intrigued08:47
cluckjthere is some dude whose lab I wanted to get into who was grinding up whipworms and giving them to people08:47
cluckjto cure a whole bunch of autoimmune conditions08:47
CheckDavidPeople reporting feeling energetic after 9 days of nutrient deprivation and sucb08:47
CheckDavidI had to try it08:47
CheckDavidThat's how intrigued I was08:48
CheckDavid*such08:48
CheckDavidBut only for like 60 hours08:48
CheckDavidLil over 48 hours08:48
CheckDavidBarely a proper experience08:48
cluckjstarvation conditions change your biochemistry08:48
CheckDavidBut still interesting08:49
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CheckDavidcluckj: can you elaborate?08:49
cluckjCheckDavid, your body basically empties out its local energy storage, then starts consuming functional proteins, then your fat cells dump all their storage08:50
Adlaiwhen an animal starves, it ceases storing fat, and begins digesting stored fat08:50
cluckjthere are some diets that can forcce your body into that kind of mode tho08:50
Adlaiiiuc you don't start consuming functional proteins until you're in quite a dilipidated state08:50
cluckjketo diets do that, where you put yourself into a state of ketogenesis08:50
cluckjAdlai, I thought so too but iirc your body does the intercellular stuff first08:51
Adlaifasting also makes you metabolize more rapidly once you ingest, so it's also used to accelerate/intensify psychoactive effects08:53
cluckjyeah08:53
cluckjwhen you do the keto diet, if you eat too many carbs you ruin the ketogenic state08:54
CheckDavidSome say muscle goes first08:54
CheckDavidOthers say fat goes first08:54
CheckDavidWhich is more accurate?08:54
cluckjI think muscle does08:55
cluckjin starvation conditions08:55
cluckjit's stupid but the human body was not intelligently designed :)08:55
cluckjand it's a temporary state08:55
cluckjit's not like you run out of muscles before your body starts eating fat stores08:56
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kanzurethere should be a muscle eating starvation curve chart08:56
cluckjthere probably is08:56
cluckjfrom my own personal experience, muscle is definitely higher on the consuming/producing curve than fat08:58
cluckjI've been trying to gain some fat back from the undiagnosed diabetes starvation conditions for like 2 years, and all I can get is useless shitty muscle09:01
cluckj(I need fatty places to stick cannulas and sensors)09:01
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CheckDaviddamn that's crazy cluckj10:03
CheckDavidSome people will be envious10:03
CheckDavidLike me10:04
CheckDavidI'm envious that you get so much useless muscle10:04
CheckDavidAnd no fat10:04
cluckjlol10:04
cluckjit was because of undiagnosed type 1 diabetes10:05
cluckjso there's a tradeoff, I guess :P10:06
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kanzure"Visualize black hole gravitational lensing in WebGL" https://github.com/cliffcrosland/black-hole.js10:20
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CheckDavidOh I see10:36
CheckDavidcluckj: I laugh at women who think they will get very large by lifting weights. They are afraid of.getting too much useless muscle10:38
CheckDavidLike you10:38
CheckDavidBut then I know you are not a women because else you wouldn't even be putting on too much muscle10:38
CheckDavidLol10:38
CheckDavidThat's the funny thing10:39
cluckjO_o10:40
kanzureuh...10:42
kanzurewat10:43
cluckj^10:43
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kanzure"They had the brain open, and every single time that they had an electrode stimulation to a very certain region of the cardiac nucleuses, which is part of this wanting system, the patient would turn around and say that he loved the doctor."10:56
andytoshithat doctor sounds lovely10:57
kanzureno you're supposed to wait until he zaps you10:58
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andytoshioh, ok10:59
andytoshii can't wait :]10:59
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nmz787this looks pretty friggin cool http://www.learnopencv.com/seamless-cloning-using-opencv-python-cpp/11:55
chris_99intriguing11:55
chris_99but i guess it only works if the object has a solid background11:56
chris_99i'm still deciding on the ADC, nmz787, maybe i should just go for something cheap to start with12:09
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nmz787chris_99: have you checked the openspectrometer specs?13:01
nmz787the ADC isn't outlandishly priced13:01
chris_99yeah that seemed a good one, the wolfson one13:02
chris_99if i just used one of those 'slide' diffraction gratings, i can just place it straight in front of the ccd right13:03
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nmz787by slide you mean transmission?13:43
chris_99yeah13:43
nmz787yep, check out the PLOTS spectrometer to see their setup instructions13:43
chris_99ah cheers13:43
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kanzurevivi: hi14:39
vivihello kanzure14:40
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* Adlai , being drunk, is now curious to solicit the channel's opinion of alcohol16:17
Adlaiit's definitely not nootropic16:17
Adlaisubhuman, and by muddling the conscious/precise mind, it interferes with bio- and hardware hacking - thus a perfect fit for this channel!16:17
kanzurelobotomies are also not often nootropic16:19
Adlaisure, but lobotomies don't write poetry, or cause desperate monkeys to ask other monkeys what they think of monkeydom16:20
Adlai(and neither do lobotomized monkeys, they tend to sit around, staring at wallS)16:21
* Adlai has been pondering the value of a university education16:27
Adlaisome of the last words out of my mouth were16:27
Adlai"i have found a bunch of people doing independent biology, so even that isn't restricted to the hallowed groves of academe anymore"16:28
Adlaiprove me wrong, please16:28
Adlai(actuallly, i'd rather you don't, but i think you know what you mean. yes, _you_)16:28
cluckj:(16:30
cluckjme?16:30
kanzureAdlai: friends don't let friends go to school16:31
cluckjhah16:31
Adlaio rly16:32
Adlaiy not?16:32
* Adlai means, that the last friends - with whom he spoke about the last words whence his mouth - urged him to study16:32
kanzurelearning can happen anywhere, and you can join labs without being a student16:32
Adlaiand by "study", /me implies at a formal institution16:32
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kanzureand besides, you're a programmer. your industry doesn't even care about credentials.16:34
cluckjI wonder why that is16:35
Adlaieh it only disregards credentials when your github is full of nodejs angular blabla16:35
* Adlai managed the "i don't have a resume but github" once, twas nice, nevar again16:35
kanzurenobody actually reads source code on github when a candidate applies. they really don't...16:35
kanzure(but yes you should still have shit there)16:36
Adlaidunno16:37
Adlaii heard "he thought your github was nice" in the first skype call16:37
Adlaidoesn't that _mean_ something?16:38
kanzureit could easily mean nothing, like "he said something positive, but i don't understand the gitz, so i am calling you to waste your time anyway instead of just having him review the quality of source code you write"16:39
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andytoshikanzure: actually i received a PM from an employer (of others) complaining that github made it hard to tell whether contributions are "real"17:08
andytoshithat is, code rather than docfixes17:08
andytoshiso he/she is at least looking as far as what shape are git commits17:08
andytoshibut maybe the people who PM me about their hiring troubles are not typical employers. *shrug*17:08
Adlaiandytoshi: IME, PMs are from recruiters17:09
Adlai(for values of personal increasingly tending towards "linked")17:09
andytoshiAdlai: fyi, people keep irc logs forever ... it's kinda fun but i make a point of never being on IRC while drunk for that reason17:11
andytoshi(not that you've said anything worrisome, just general advice)17:11
* Adlai has seen far worse17:11
Adlaisome IRC networks are built around drugs. imagine how #stims on irc.tripsit.me looks...17:12
andytoshisure, but if you're looking to do research/development stuff under this name with people on this network, there's a benefit to being more cautious. *shrug again*17:13
kanzureandytoshi: git commits can be forged too, but whatever17:13
* Adlai shuts up?17:14
andytoshikanzure: this is true, but i think the skill needed to realize that is also a barrier..17:17
andytoshiidk, maybe it'd be more common if it was more useful17:18
andytoshiright now you can post "trololol" in the Issues section of a million projects and github will say you've "contributed to" them all17:18
Adlaiyes, but forging _history_ is more difficult17:18
Adlai(and requires getting pulled, or convincing your potential employers that your nonsense really is worth something)17:19
andytoshiAdlai: well, you need push access to whatever repo you want that history to be in...but the actual forgery is easy enough17:20
andytoshilike you can just put your name on every commit17:20
* Adlai has several times told himself that correcting trailing whitespace in widely used open source projects is not worth the github profile boost that it would yield17:20
Adlaiplease, show me the recruiter than would notice.17:21
andytoshibtw kanzure, it took me all of five minutes to set up a new server running modern software (i'm migrating files right now), thx for the push to do it..17:34
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kanzurecool17:37
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kanzure"Before bitcoin [ransomware] was done with Moneypack, Western Union, and others"17:55
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kanzuresomewhat more popularized fermi paradox explanation post http://waitbutwhy.com/2014/05/fermi-paradox.html18:46
adlaiwe have no way of directly inspecting "the multiverse"... maybe "life" (ie consciousness capable of conceiving such paradoxen) is much much rarerererer than expected, and by the time/verse it arises, is alone to wonder about this?18:49
kanzureadlai: one interesting theory is that binary pulsars are probably alien civilizatios18:49
kanzurealien civilizations18:49
* adlai hopes that any aliens capable of reaching us with signals would be capable of a) being reached by ours, and b) understanding how to adapt their signals to be parsable by our admittedly humble intelligence18:50
adlaiit could also be that "nobody cares", it's a simulation/anomaly/whateveryoumakeofit18:51
adlaithese are all equivalent, from within.18:51
adlaimaybe i'm expecting the communication counterparty to slow down for my benefit, a sin equal to expecting your audience to "keep up" (an accusation recently levelled against an author using too much math in a paper intended for mathematicians)18:53
adlai... but why shouldn't non-interplanetary worms on a rock in space hope that their elders give them a helping hand?18:54
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kanzurehttp://www.piratehearts.com/blog/2014/03/28/crt-simulation/19:13
streetypaperbot http://www.sciencedirect.com/science/article/pii/S095656631500201819:18
kanzure.title19:18
yoleauxMicrofluidic enzymatic biosensing systems: A review19:18
paperbothttp://diyhpl.us/~bryan/papers2/paperbot/%0A%20Microfluidic%20enzymatic%20biosensing%20systems%3A%20A%20review%0A%20.pdf19:19
kanzurepaperbot: http://www.sciencedirect.com/science/article/pii/S0956566315002018/pdfft?md5=74aa65b3289041cdf3bdd4154bb8a5fb&pid=1-s2.0-S0956566315002018-main.pdf19:19
paperbothttp://diyhpl.us/~bryan/papers2/paperbot/6062d8ae911843d1978167384447d54a.pdf19:19
streetythanks19:21
adlaikanzure: that crt simulation is skeumorphism taken to an impressive/worrying extreme19:34
kanzureisn't that the channel moto? "take everything to worrying extremes"/19:35
adlai/motto19:35
kanzureah yes19:35
* adlai must've lepht something behind19:36
kanzureugh that person?19:36
adlaidunno, you said worrying extreme19:36
kanzurelepht is a person19:36
adlaiwho, according to links from /motto, is extreme19:37
kanzureuh?19:40
adlaiwell i guess it's not linked from teh topic, but from the wiki.19:40
kanzureshe's just pandering to a boring audience who don't understand magnets or engineering19:42
adlaithem's a she now?19:43
* adlai means no jest, trying to make sense19:44
kanzurelepht is a person19:44
kanzureher audience is a group of people19:44
adlaiok19:45
* adlai wonders if any biohackers have tackled sleep yet, or is that left for after the BRAIN initiative19:45
kanzureintroducing the most boring tool for scientific pdfs http://www.docear.org/ (really, this makes no sense. sounds super boring.)19:59
kanzure.title https://news.ycombinator.com/item?id=933431620:27
yoleauxThe future of the postdoc | Hacker News20:27
cluckjtenure or bust20:35
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--- Log closed Mon Apr 13 00:00:51 2015

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