| --- Log opened Fri Aug 14 00:00:40 2015 | ||
| -!- sheena [~home@S0106c8be196316d1.ok.shawcable.net] has joined ##hplusroadmap | 00:15 | |
| nmz787 | the price is not terribly student friendly: http://www.edn.com/design/design-tools/development-kits/4440137/Student-version-of-MATLAB-Simulink-drives-Raspberry-Pi-to-new-heights | 00:36 |
|---|---|---|
| -!- sheena [~home@S0106c8be196316d1.ok.shawcable.net] has quit [Ping timeout: 244 seconds] | 01:03 | |
| -!- xtalmath [~xtalmath@ip-83-134-138-89.dsl.scarlet.be] has quit [Ping timeout: 250 seconds] | 01:07 | |
| -!- xtalmath [~xtalmath@ip-81-11-174-236.dsl.scarlet.be] has joined ##hplusroadmap | 01:23 | |
| nmz787 | kanzure: that intel history link is pretty awesome, especially since I am working on test equipment development | 01:25 |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-avygleirxrxsnzul] has joined ##hplusroadmap | 01:48 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 246 seconds] | 02:14 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 02:23 | |
| -!- fleshtheworld- [~fleshthew@2602:306:cf0f:4c20:59a2:6118:4daf:3141] has quit [Read error: Connection reset by peer] | 03:06 | |
| -!- wrldpc1 [~ben@133.131.169.216.client.dyn.strong-sf94.as22781.net] has quit [Quit: wrldpc1] | 03:27 | |
| -!- c0rw|zZz is now known as c0rw1n | 03:27 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-avygleirxrxsnzul] has quit [Quit: Connection closed for inactivity] | 03:57 | |
| kanzure | https://www.reddit.com/r/chemistry/comments/3gx7kg/seeking_collaborators_for_a_selffunded/ | 04:05 |
| gradstudentbot | No no no no! Use your key commands! | 04:07 |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 04:11 | |
| -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-fmkpalsssqbcylap] has quit [Quit: Connection closed for inactivity] | 04:48 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 04:49 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 04:50 | |
| -!- poppingtonic [brian@unaffiliated/poppingtonic] has joined ##hplusroadmap | 05:37 | |
| -!- Dumpster_D1ver [~Vishnu@pool-72-83-63-252.washdc.east.verizon.net] has quit [Quit: Leaving] | 05:44 | |
| -!- poppingtonic [brian@unaffiliated/poppingtonic] has left ##hplusroadmap ["ERC Version 5.3 (IRC client for Emacs)"] | 05:54 | |
| -!- eudoxia [~eudoxia@r167-57-89-176.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 05:55 | |
| -!- eudoxia [~eudoxia@r167-57-89-176.dialup.adsl.anteldata.net.uy] has quit [Client Quit] | 05:55 | |
| -!- eudoxia [~eudoxia@r167-57-89-176.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 05:56 | |
| -!- wrldpc1 [~ben@235.106.1.110.ap.yournet.ne.jp] has joined ##hplusroadmap | 06:05 | |
| -!- CaptHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap | 06:22 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-quuhcdgzppxwwkcg] has joined ##hplusroadmap | 06:43 | |
| -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has quit [Ping timeout: 272 seconds] | 06:44 | |
| -!- CyberelfJess [~CyberelfJ@ip565f6f48.direct-adsl.nl] has joined ##hplusroadmap | 07:23 | |
| kanzure | greetings CyberelfJess | 07:36 |
| fenn | who is trollzyme? | 07:41 |
| fenn | or rather, why use a new account | 07:41 |
| -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has joined ##hplusroadmap | 07:42 | |
| kanzure | one-directional privacy | 07:43 |
| fenn | "There are already DNA synthesizers on the market that work nicely" lol yeah right | 07:44 |
| fenn | efficient market fallacy | 07:45 |
| kanzure | praise unto the market | 07:45 |
| kanzure | take the wheel, supply side jesus | 07:45 |
| fenn | some photos from that port explosion http://pixel.nymag.com/imgs/daily/intelligencer/2015/08/13/tianjin-explosion/tianjin-china-explosion13.w529.h352.2x.jpg http://pixel.nymag.com/imgs/daily/intelligencer/2015/08/13/tianjin-explosion/tianjin-china-explosion11.w529.h352.2x.jpg http://pixel.nymag.com/imgs/daily/intelligencer/2015/08/13/tianjin-explosion/tianjin-china-explosion05.w529.h352.2x.jpg | 07:49 |
| kanzure | it's weird how you have to argue for every inch everywhere you go even if you're offering money | 07:55 |
| kanzure | just once i'd rather not explain the entire philosophical context of everything ever | 07:55 |
| -!- mgin [~mgin@unaffiliated/mgin] has joined ##hplusroadmap | 07:58 | |
| xtalmath | that clip just stays so incredible https://www.youtube.com/watch?v=H_nnZntSYjo | 08:09 |
| kanzure | .title | 08:11 |
| yoleaux | The Tianjin Explosion - Stabilized - YouTube | 08:11 |
| JayDugger | Good morning, everyone. | 08:22 |
| kanzure | are you guys done with your disaster porn yet | 08:24 |
| xtalmath | its not disaster porn, its a reminder of how important safety precautions are | 08:25 |
| * fenn straps on a hard hat in case oakland explodes | 08:42 | |
| -!- maaku [~quassel@50-0-37-37.dsl.static.fusionbroadband.com] has quit [Ping timeout: 246 seconds] | 08:52 | |
| -!- maaku [~quassel@50-0-37-37.dsl.static.fusionbroadband.com] has joined ##hplusroadmap | 08:54 | |
| -!- maaku is now known as Guest68235 | 08:55 | |
| -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap | 09:00 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has joined ##hplusroadmap | 09:05 | |
| -!- Merovoth [~Merovoth@unaffiliated/merovoth] has quit [] | 09:07 | |
| -!- eudoxia [~eudoxia@r167-57-89-176.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 09:07 | |
| -!- c0rw1n [~c0rw1n@91.176.86.226] has quit [] | 09:08 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 09:09 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 09:09 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Max SendQ exceeded] | 09:09 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 09:09 | |
| -!- c0rw1n [~c0rw1n@91.176.86.226] has joined ##hplusroadmap | 09:12 | |
| -!- CyberelfJess [~CyberelfJ@ip565f6f48.direct-adsl.nl] has quit [Ping timeout: 250 seconds] | 09:26 | |
| -!- Dumpster_Diver [~Vishnu@pool-72-83-63-252.washdc.east.verizon.net] has joined ##hplusroadmap | 09:39 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:8c11:6b3:2bcc:57c] has joined ##hplusroadmap | 09:44 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 252 seconds] | 10:25 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 10:28 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 10:28 | |
| -!- eudoxia [~eudoxia@r167-57-89-176.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 10:30 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 10:34 | |
| -!- CyberelfJess [~CyberelfJ@ip565f6f48.direct-adsl.nl] has joined ##hplusroadmap | 10:34 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has quit [Ping timeout: 265 seconds] | 10:35 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has joined ##hplusroadmap | 10:41 | |
| -!- c0rw1n [~c0rw1n@91.176.86.226] has quit [Read error: Connection reset by peer] | 10:46 | |
| -!- c0rw1n [~c0rw1n@91.176.86.226] has joined ##hplusroadmap | 10:47 | |
| -!- juri_ [~juri@vpn166.sdf.org] has quit [Ping timeout: 252 seconds] | 11:01 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 11:04 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 11:04 | |
| -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-hhzszmmyihvvsbyw] has joined ##hplusroadmap | 11:16 | |
| -!- eudoxia [~eudoxia@r167-57-89-176.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 11:36 | |
| kanzure | beep boop | 11:38 |
| -!- Merovoth [~Merovoth@unaffiliated/merovoth] has joined ##hplusroadmap | 11:49 | |
| chris_99 | i'm wondering if anyone can point me in the right direction, i'm going to try to automate the stage of my microscope which i think i've figured out how to do roughly now, i'm going to be driving the steppers via DRV8825 using an STM32F429 dev board, attached to my PC via USB | 11:55 |
| chris_99 | i'm gonna write some very simple software for the devboard, but i'm wondering if theres some fairly simple PC software i could use to control everything | 11:56 |
| kanzure | linuxcnc | 11:57 |
| chris_99 | so i just looked at their page, isn't it a full distro though? | 11:57 |
| kanzure | i dunno if you can use linuxcnc without realtime mode, but my guess is probably, so just throw it in a virtual machine | 11:57 |
| chris_99 | i was wondering if theres not a core piece of their distro i could just install instead, or is it all heavily interlinked | 11:59 |
| kanzure | i'm not the right person to ask, try fenn or CaptHindsight or #linuxcnc | 12:01 |
| chris_99 | ta, i'll try in their channel | 12:02 |
| fenn | use grbl and arduino unless you specifically need the dev board | 12:02 |
| chris_99 | i don't have any arduinos | 12:03 |
| fenn | USB is terrible for motion control and negates the benefit of linuxcnc | 12:03 |
| fenn | also i don't think linuxcnc even works with any usb libraries because the devs hate it | 12:03 |
| chris_99 | ah heh | 12:03 |
| fenn | actually that's not right, but it will be hard to set up something controlling motion via usb | 12:04 |
| kanzure | ah i see | 12:04 |
| kanzure | i was hoping that using linuxcnc would make up for the horribleness of usb | 12:04 |
| fenn | no, it's the other way around | 12:04 |
| fenn | usb is so terrible even linuxcnc can't fix it | 12:04 |
| kanzure | some sort of net project karmic measurement | 12:04 |
| kanzure | ah i see. | 12:04 |
| chris_99 | what other candidates are there that i could use on a laptop, i can only think of ethernet, but is that much better | 12:05 |
| fenn | just buy an atmega328 for $5 sheesh | 12:06 |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 12:06 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 12:06 | |
| chris_99 | i don't want to use arduinos though :P | 12:06 |
| kanzure | fenn: did you hang out with t12 by any chance? | 12:09 |
| fenn | no | 12:10 |
| kanzure | got a reply about cambrian genomics.. at least they are topical. | 12:14 |
| kanzure | having trouble figuring out why i know P212121.. he's not in my stalkmatrix, but i know about p212121.com? | 12:14 |
| xtalmath | fenn: you propose connecting an arduino through something else than USB to the computer? | 12:23 |
| fenn | the arduino would be running the timing-sensitive motion control software | 12:24 |
| kanzure | obviously you should use gsm https://www.usenix.org/system/files/conference/usenixsecurity15/sec15-paper-guri-update.pdf | 12:24 |
| chris_99 | heh is that the gsmem paper | 12:24 |
| xtalmath | chris_99: what kind of motion control do you need? CNC & closed loop feedback? or just some pan left/right/up/down where it doesnt matter if it overshot a little? | 12:24 |
| gradstudentbot | When is he back from sabbatical? | 12:24 |
| fenn | it's stepper motors so open loop | 12:25 |
| kanzure | yes that is the gsmem paper | 12:25 |
| xtalmath | fenn: what is the application? direct write or viewing slides? | 12:25 |
| chris_99 | oh it's to image chips | 12:25 |
| fenn | fuck if i know | 12:25 |
| * fenn goes away and stews | 12:26 | |
| xtalmath | chris_99: then I can't imagine the setup you propose to be troublesome in the sense of needing tight path control | 12:26 |
| xtalmath | chris_99: the important imaging will always happen when the stage is stationary? | 12:27 |
| chris_99 | yeah | 12:28 |
| xtalmath | then why sweat about such an issue? | 12:28 |
| xtalmath | or your question was more general? if so, I don't have enough experience/data to see if your components are suitable for your steppers... | 12:29 |
| -!- nmz787_w [86868b4a@gateway/web/freenode/ip.134.134.139.74] has joined ##hplusroadmap | 12:30 | |
| chris_99 | i'm also wondering for other cnc stuff too, just asking in #linuxcnc re. I/O mechanism | 12:30 |
| nmz787_w | kanzure: wiley error dump showing some python stuff http://pastebin.com/pnVzq91p | 12:31 |
| -!- EnLilaSko- [~Nattzor@host-85-30-145-65.sydskane.nu] has joined ##hplusroadmap | 12:31 | |
| nmz787_w | kanzure: when I hit this link http://onlinelibrary.wiley.com/store/10.1002/anie.201501779/asset/supinfo/anie_201501779_sm_miscellaneous_information.pdf?v=1&s=7a2594f665c25453d028c5a73dba9b33359563c8 | 12:31 |
| nmz787_w | kanzure: coming from this accessible link http://onlinelibrary.wiley.com/doi/10.1002/anie.201501779/suppinfo | 12:32 |
| kanzure | thank you | 12:32 |
| kanzure | mod_python haha | 12:33 |
| -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has quit [Ping timeout: 250 seconds] | 12:33 | |
| -!- EnLilaSko- is now known as EnLilaSko | 12:33 | |
| -!- EnLilaSko [~Nattzor@host-85-30-145-65.sydskane.nu] has quit [Changing host] | 12:33 | |
| -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap | 12:33 | |
| kanzure | that pdf loads for me | 12:33 |
| -!- souljack [souljack@shell.xshellz.com] has joined ##hplusroadmap | 12:43 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:8c11:6b3:2bcc:57c] has quit [Ping timeout: 246 seconds] | 12:46 | |
| nmz787_w | kanzure: anything interesting in the PDF, still doesn't work here | 12:49 |
| nmz787_w | i'm guessing nothing too interesting... since the main paper doesn't seem to indicate any kind of DNA work | 12:53 |
| nmz787_w | interesting to me | 12:53 |
| -!- CyberelfJess [~CyberelfJ@ip565f6f48.direct-adsl.nl] has quit [Ping timeout: 244 seconds] | 12:56 | |
| -!- sandeep [~sandeep@111.235.64.135] has joined ##hplusroadmap | 12:58 | |
| -!- sandeep is now known as Guest22866 | 12:58 | |
| -!- Guest22866 [~sandeep@111.235.64.135] has quit [Client Quit] | 13:00 | |
| -!- sandeep_ [~sandeep@111.235.64.135] has joined ##hplusroadmap | 13:00 | |
| -!- sheena [~home@S0106c8be196316d1.ok.shawcable.net] has joined ##hplusroadmap | 13:04 | |
| -!- Adlai [~Adlai@unaffiliated/adlai] has quit [Quit: Insufficient entropy for original quit message] | 13:26 | |
| -!- Dumpster_Diver [~Vishnu@pool-72-83-63-252.washdc.east.verizon.net] has quit [Ping timeout: 246 seconds] | 13:30 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:bcb9:2d04:30ed:c855] has joined ##hplusroadmap | 13:34 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has quit [Ping timeout: 246 seconds] | 13:43 | |
| -!- FourFire [~FourFire@185.7.192.138] has joined ##hplusroadmap | 13:51 | |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-zrrdtgqlxmxpfjcl] has joined ##hplusroadmap | 14:05 | |
| -!- Houshalter [~Houshalte@cpe-65-24-174-129.columbus.res.rr.com] has joined ##hplusroadmap | 14:21 | |
| -!- xtalmath [~xtalmath@ip-81-11-174-236.dsl.scarlet.be] has quit [Quit: Leaving.] | 14:26 | |
| -!- Adlai [~Adlai@unaffiliated/adlai] has joined ##hplusroadmap | 14:27 | |
| justanotheruser | okay, what happens today | 14:39 |
| kanzure | "oh that sounds like an engineering problem" | 15:02 |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 15:03 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 15:03 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 15:03 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 15:04 | |
| kanzure | "just call glenn research's tech support line, they will tell you all the details" | 15:04 |
| kanzure | er, glen research's | 15:05 |
| -!- Dumpster_Diver [~Vishnu@96.88.198.77] has joined ##hplusroadmap | 15:26 | |
| -!- Houshalter [~Houshalte@cpe-65-24-174-129.columbus.res.rr.com] has quit [Ping timeout: 244 seconds] | 15:28 | |
| -!- Houshalter [~Houshalte@cpe-65-24-174-129.columbus.res.rr.com] has joined ##hplusroadmap | 15:29 | |
| -!- Dumpster_Diver [~Vishnu@96.88.198.77] has quit [Ping timeout: 255 seconds] | 15:32 | |
| -!- FourFire [~FourFire@185.7.192.138] has quit [Ping timeout: 255 seconds] | 15:33 | |
| -!- Dumpster_Diver [~Vishnu@96.88.198.77] has joined ##hplusroadmap | 15:46 | |
| -!- erasmus [~esb@unaffiliated/erasmus] has quit [Read error: Connection reset by peer] | 15:52 | |
| -!- AmbulatoryCortex [~Ambulator@173-31-155-69.client.mchsi.com] has joined ##hplusroadmap | 16:06 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Ex-Chat] | 16:21 | |
| -!- Dumpster_Diver [~Vishnu@96.88.198.77] has quit [Ping timeout: 264 seconds] | 16:39 | |
| -!- yashgaroth [~ffffff@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has joined ##hplusroadmap | 16:56 | |
| jrayhawk | what's funny about mod_python | 17:03 |
| kanzure | really you should be using mod_wsgi | 17:06 |
| jrayhawk | why | 17:06 |
| jrayhawk | unlike most web developers, i can actually read apache documentation and not run the damned thing in prefork | 17:07 |
| jrayhawk | not that there's clear benefits either way | 17:07 |
| jrayhawk | either way on mod_wsgi vs. mod_python | 17:08 |
| jrayhawk | mod_wsgi gets more scheduling pressure, mod_python gets interpreter overhead | 17:08 |
| kanzure | hm.. | 17:08 |
| jrayhawk | the scheduling pressure is extra funny when apache starts bouncing between minsparethreads and maxrequestworkers/threadlimit on wsgi | 17:13 |
| jrayhawk | or the prefork equivalent | 17:13 |
| jrayhawk | so web developers first convince themselves that mod_python is useless because they don't know how to configure apache, then they convince themselves that mod_wsgi is useless because they don't know how to configure apache | 17:14 |
| jrayhawk | so then they switch to nginx | 17:14 |
| jrayhawk | i will say doing privilege separation without wsgi is pretty horrible | 17:18 |
| jrayhawk | possible, but horrible | 17:18 |
| kanzure | surely it's not an apache configuration issue | 17:21 |
| jrayhawk | apache makes sane privilege separation incredibly hard | 17:24 |
| jrayhawk | nginx "solves" this problem by just not supporting dynamic execution | 17:25 |
| -!- Guest68235 is now known as maaku | 17:27 | |
| jrayhawk | which i guess is a good thing in an Apple sort of design philosophy paradigm | 17:33 |
| gradstudentbot | Should this be on ice? | 17:35 |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-zrrdtgqlxmxpfjcl] has quit [Quit: Connection closed for inactivity] | 17:46 | |
| -!- strangewarp_ [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Quit: austerity chic brand destruction] | 17:56 | |
| -!- nmz787_w [86868b4a@gateway/web/freenode/ip.134.134.139.74] has quit [Ping timeout: 246 seconds] | 18:04 | |
| kanzure | http://www.glenresearch.com/Reference/oligohouses.html | 18:05 |
| -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap | 18:10 | |
| kanzure | http://www.glenresearch.com/Reference/background.html | 18:15 |
| kanzure | "As we endeavor to improve on existing products for DNA synthesis, we focus on areas of weakness in the synthesis process. Our work on the redesign of synthesis columns has culminated in the development of TWIST™ columns for routine synthesis. In addition to being virtually leak-proof, TWIST columns allow users, if necessary, to gain access to the support simply by twisting off the retaining cap." | 18:16 |
| kanzure | "Purification and isolation of synthetic oligodeoxynucleotides remains the most time-consuming and labor-intensive portion of the synthesis process. We are happy to have participated in alleviating this production bottleneck with the introduction of the Poly-Pak™ group of DNA purification products. Poly-Pak cartridges allow users to purify and isolate synthetic oligos in a fraction of the time needed in the past. This technique has ... | 18:16 |
| kanzure | ... gained popularity with core facilities, commercial producers, as well as with individual researchers. Glen Research supports the technique with a comprehensive User Guide to DNA Purification." | 18:17 |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-quuhcdgzppxwwkcg] has quit [Quit: Connection closed for inactivity] | 18:17 | |
| kanzure | "Ultrafast DNA synthesis - 10 minute deprotection" (december 1993) http://www.glenresearch.com/GlenReports/GR6-22.html | 18:19 |
| kanzure | "Non-enzymatic ligation of single-stranded and duplex DNA" http://www.glenresearch.com/GlenReports/GR10-18.html | 18:23 |
| kanzure | an announcement about the mermade synthesizer http://www.glenresearch.com/GlenReports/GR12-14.html | 18:24 |
| kanzure | "which chemical phosphorylation reagent?" http://www.glenresearch.com/GlenReports/GR12-16.html | 18:24 |
| kanzure | "A rapid method for atomic mutagenesis of nucleic acids" http://www.glenresearch.com/GlenReports/GR13-12.html | 18:25 |
| kanzure | 96-well 30-mer synthesizer http://www.glenresearch.com/GlenReports/GR13-14.html (polyplex synthesizer / genemachines) | 18:25 |
| kanzure | "Trityl groups in the 3rd millenium" http://www.glenresearch.com/GlenReports/GR13-13.html <--- this looks good | 18:26 |
| kanzure | photocleavable biotin http://www.glenresearch.com/GlenReports/GR14-14.html | 18:27 |
| kanzure | preparation of methyl triester linkages http://www.glenresearch.com/GlenReports/GR14-16.html | 18:27 |
| kanzure | immobilizing oligos to gold surfaces using thiol anchors http://www.glenresearch.com/GlenReports/GR16-12.html | 18:28 |
| kanzure | "Unicap phosphoramidite, an alternative to acetic anhydride capping" http://www.glenresearch.com/GlenReports/GR17-13.html | 18:29 |
| kanzure | "A detailed comparison of seven commercially available universal supports, which have been examined for their ability to support the synthesis of short and long oligodeoxynucleotides (DNA oligomers) and also oligoribonucleotides (RNA oligomers), is reported herein." http://www.glenresearch.com/GlenReports/GR17-2.html | 18:31 |
| kanzure | "Precautions during packaging of phosphoramidites and transfers into alternate vials" http://www.glenresearch.com/GlenReports/GR19-13.html | 18:32 |
| kanzure | hmm looks like they do a lot of work with ellington | 18:33 |
| kanzure | preventing impurities http://www.glenresearch.com/GlenReports/GR19-18.html | 18:34 |
| kanzure | synthesis and deprotection of synthetic RNA http://www.glenresearch.com/GlenReports/GR19-22.html | 18:34 |
| kanzure | tetrazole and other activators http://www.glenresearch.com/GlenReports/GR19-29.html | 18:34 |
| kanzure | phosphonoacetate oligonucleotides with enhanced nuclease resistance http://www.glenresearch.com/GlenReports/GR20-21.html and http://www.glenresearch.com/GlenReports/GR20-2CONT.html | 18:36 |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 255 seconds] | 18:36 | |
| kanzure | optimal deprotection strategies http://www.glenresearch.com/GlenReports/GR20-24.html | 18:36 |
| kanzure | avoiding trityl loss during oligonucleotide synthesis http://www.glenresearch.com/GlenReports/GR21-19.html | 18:38 |
| kanzure | azobenzene phosphoramidite http://www.glenresearch.com/GlenReports/GR21-27.html (cute) | 18:38 |
| kanzure | "Synthesis of long oligonucleotides" http://www.glenresearch.com/GlenReports/GR21-211.html | 18:39 |
| kanzure | "Alternatives to ammonium hydroxide for deprotection" http://www.glenresearch.com/GlenReports/GR22-18.html | 18:40 |
| kanzure | depurination http://www.glenresearch.com/GlenReports/GR22-19.html | 18:40 |
| kanzure | chemical phosphorylation http://www.glenresearch.com/GlenReports/GR23-17.html | 18:41 |
| kanzure | "2-Amino-dA is very susceptible to depurination if electron withdrawing protecting groups, for example acyl protection groups, are used. To stabilize the glycosidic bond, strongly electron donating formamidine protecting groups are used in this monomer. We surmise that the N2 formamidine group can activate the N3, which attacks the 3' carbon to displace the phosphonium iodide intermediate, leading to chain scission." ... | 18:42 |
| kanzure | ... http://www.glenresearch.com/GlenReports/GR23-28.html | 18:42 |
| kanzure | "Ubiqitous reagent for deprotection" http://www.glenresearch.com/GlenReports/GR26-14.html | 18:45 |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 18:46 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 18:46 | |
| kanzure | "Reversible photoswitching of DNA function with azobenzene-tethered DNA" http://www.glenresearch.com/GlenReports/GR27-11.html | 18:46 |
| kanzure | "30% ammonium hydroxide/propylamine/water 2:1:1 (v/v/v) for deprotection" http://www.glenresearch.com/GlenReports/GR27-13.html | 18:47 |
| kanzure | "However, methylamine does have a downside, in that it is used as a reagent in the illicit production of methamphetamine.2 As a result, it is a DEA-regulated substance and in California additional regulatory hurdles have been implemented on the purchase and use of methylamine solutions and salts, making it difficult to procure quickly or easily.3 As a result, we investigated alternative alkylamines that are not under the DEA's list of ... | 18:47 |
| kanzure | ... regulated chemicals for our Californian customers. The best candidate we found was propylamine. Propylamine is a liquid at room temperature, boiling at 48 °C, and is miscible with water. However, we found that adding it directly to 30% ammonium hydroxide led to significant degassing of ammonia from the solution. To avoid this, water was added to the solution yielding the final formulation of APA as 30% ammonium ... | 18:47 |
| kanzure | ... hydroxide/propylamine/water 2:1:1 (v/v/v)." | 18:47 |
| -!- c0rw1n is now known as c0rw|zZz | 19:15 | |
| -!- CaptHindsight [~2020@unaffiliated/capthindsight] has quit [Read error: Connection reset by peer] | 19:40 | |
| -!- AmbulatoryCortex [~Ambulator@173-31-155-69.client.mchsi.com] has quit [Read error: Connection reset by peer] | 19:48 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 244 seconds] | 19:51 | |
| -!- Houshalter [~Houshalte@cpe-65-24-174-129.columbus.res.rr.com] has quit [Ping timeout: 265 seconds] | 20:11 | |
| -!- wrldpc1 [~ben@235.106.1.110.ap.yournet.ne.jp] has quit [Quit: wrldpc1] | 20:25 | |
| -!- drewbot [~cinch@ec2-54-82-50-74.compute-1.amazonaws.com] has quit [Remote host closed the connection] | 20:56 | |
| -!- drewbot [~cinch@ec2-54-90-92-35.compute-1.amazonaws.com] has joined ##hplusroadmap | 20:56 | |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 20:59 | |
| -!- xtalmath [~xtalmath@ip-81-11-174-236.dsl.scarlet.be] has joined ##hplusroadmap | 21:08 | |
| -!- xtalmath [~xtalmath@ip-81-11-174-236.dsl.scarlet.be] has quit [Quit: Leaving.] | 21:34 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-amswctqhojyxrvqg] has joined ##hplusroadmap | 21:37 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has joined ##hplusroadmap | 21:47 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has quit [Client Quit] | 21:48 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has joined ##hplusroadmap | 21:49 | |
| -!- CaptHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap | 21:59 | |
| -!- Houshalter [~Houshalte@oh-71-50-58-200.dhcp.embarqhsd.net] has quit [Quit: Quit] | 22:04 | |
| -!- juri_ [~juri@vpn166.sdf.org] has joined ##hplusroadmap | 22:17 | |
| -!- yashgaroth [~ffffff@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has quit [Quit: Leaving] | 22:38 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 244 seconds] | 22:44 | |
| -!- joshcryer [~g@unaffiliated/joshcryer] has joined ##hplusroadmap | 23:17 | |
| juri_ | http://www.sciencemag.org/content/349/6249/677.full | 23:19 |
| -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap | 23:38 | |
| -!- drethelin [drethelin@71-87-115-157.dhcp.mdsn.wi.charter.com] has quit [] | 23:43 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:bcb9:2d04:30ed:c855] has quit [Quit: Leaving] | 23:55 | |
| --- Log closed Sat Aug 15 00:00:41 2015 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!
