| --- Log opened Thu Sep 24 00:00:37 2015 | ||
| nmz787 | CaptHindsight: yep, to arrage the cells... I wonder if you could alternatively use meat-glue (transglutaminase, I think) | 00:05 |
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| nmz787 | .tell chris_99 I just got the new mini laser etcher to cut via wine in ubuntu and something like this : ln -s /dev/ttyUSB0 ~/.wine/dosdevices/com1 (I shouldn't have even messed with trying on Windows 8, no driver support, was a big waste of time... I shouldn't have even rebooted) | 01:19 |
| yoleaux | nmz787: I'll pass your message to chris_99. | 01:19 |
| nmz787 | .tell chris_99 driver worked out of the box on ubuntu | 01:20 |
| yoleaux | nmz787: I'll pass your message to chris_99. | 01:20 |
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| kanzure | apparently skin transplants from cadavers is a thing that works | 04:15 |
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| kanzure | http://www.ncbi.nlm.nih.gov/pubmed/26274315 | 04:57 |
| kanzure | "The present study provides evidence that social threat images are differentiated from nonsocial threat stimuli more quickly in the lonely (~116 ms after stimulus onset) than nonlonely (~252 ms after stimulus onset) brains." | 04:58 |
| justanotheruser | All-cause mortality was 5.85 per 10,000 person–years among people | 05:06 |
| justanotheruser | with ADHD, compared with 2.21 per 10,000 person–years in people without ADHD. The risk of death | 05:06 |
| justanotheruser | among people with ADHD was more than double that in the general population (adjusted mortality rate | 05:06 |
| justanotheruser | ratio [MRR]=2.07, 95% confidence interval [CI] 1.70 to 2.50, p<0.0001). | 05:06 |
| justanotheruser | http://www.nice.org.uk/Media/Default/newsletter/Eyes-on-Evidence-September-2015.pdf | 05:06 |
| kanzure | doesn't show male mortality and male non-drug/with-drug mortality, which would be necessary to see if male adhd non-drug is an anomaly or not (less all-cause mortality) | 05:12 |
| justanotheruser | because being male is a predictor for ADHD? | 05:13 |
| kanzure | nah i mean https://news.ycombinator.com/item?id=10270786 | 05:14 |
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| pasky | abetusk: didn't figure out how to tear out a connection in webtronix (right click -> delete didn't do the trick) | 05:52 |
| kanzure | jojack has updated design of http://www.biotechnbeyond.com/ | 06:05 |
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| kanzure | .title http://www.sciencedirect.com/science/article/pii/S0028393206004155 | 07:33 |
| yoleaux | Intelligence and salivary testosterone levels in prepubertal children | 07:33 |
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| kanzure | https://www.quantamagazine.org/20150922-nima-arkani-hamed-collider-physics/#top | 08:09 |
| kanzure | "There is a tricky interaction between having goals and being intelligent: intelligence can be defined as the ability to achieve goals in general circumstances, so to be intelligent you need to have some kind of goals. But clearly some goals are special: even I can write an AI whose goal is to not do anything, but I think we can agree it's intelligence is not interesting. So interesting intelligence requires nontrivial goals - so in that ... | 08:13 |
| kanzure | ... space of minds there will be some correlation between being goal-oriented and intelligent. But some agents may not have an entirely goal-oriented architecture, yet be pretty good at achieving what we call goals." | 08:13 |
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| maaku | kanzure: pretty sure humans are not actually goal-oriented under the hood | 09:57 |
| maaku | there's some severe compsci-bias in that quote | 09:57 |
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| pasky | maaku: that's in line with the quote last sentence, isn't it? :) | 10:07 |
| maaku | true | 10:07 |
| kanzure | quote was from anders sandberg on extropy-chat recently | 10:09 |
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| maaku | oh sandberg, that explains it | 10:27 |
| maaku | kanzure: is that list worth subscribing to? | 10:28 |
| kanzure | not anymore | 10:43 |
| kanzure | .title http://grants.nih.gov/grants/guide/pa-files/PAR-15-345.html | 10:48 |
| yoleaux | PAR-15-345: BRAIN Initiative: Pre-Applications for Industry Partnerships to Provide Early Access to Devices for Stimulation and Recording in the Human Central Nervous System (X02) | 10:48 |
| kanzure | .title http://grants.nih.gov/grants/guide/rfa-files/RFA-NS-16-009.html | 10:48 |
| yoleaux | RFA-NS-16-009: BRAIN Initiative: Next-Generation Invasive Devices for Recording and Modulation in the Human Central Nervous System (UG3/UH3) | 10:48 |
| kanzure | .title http://grants.nih.gov/grants/guide/rfa-files/RFA-NS-16-010.html | 10:48 |
| yoleaux | RFA-NS-16-010: BRAIN Initiative: Clinical Studies to Advance Next-Generation Invasive Devices for Recording and Modulation in the Human Central Nervous System (UH3) | 10:48 |
| kanzure | http://braininitiative.nih.gov/pdf/BRAIN_PPP_CRA_9172015_508C.pdf | 10:48 |
| kanzure | .title http://braininitiative.nih.gov/BRAIN_PPP/ | 10:49 |
| kanzure | hmph | 10:49 |
| yoleaux | kanzure: Sorry, that command (.title) took too long to process. | 10:49 |
| kanzure | once we figure out memory engrams and the related methylation there, someone should bother to speculate about a brain-computer interface that works over longer time-scales (like through methylation or whatever engram techniques require) instead of neuron membrane potentials. | 10:51 |
| justanotheruser | neat http://glowforge.com/ | 10:57 |
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| justanotheruser | oh, actually not so neat | 10:59 |
| justanotheruser | "It's not just cloud connected, it's cloud dependent. It requires internet access and their cloud services to function at all. That's kind of disappointing. | 10:59 |
| kanzure | also overpriced | 11:00 |
| kanzure | wake me up when they are selling a 2m^2 100 kW laser cutter | 11:00 |
| kanzure | no wait, 100 kW solid state laser cutter | 11:00 |
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| kanzure | CaptHindsight: 100kW solid state laser plzfind | 11:01 |
| kanzure | for laser cutting | 11:01 |
| CaptHindsight | portable and pocket sized? | 11:02 |
| kanzure | nah | 11:02 |
| CaptHindsight | http://physicsworld.com/cws/article/news/2009/mar/26/dangled-dna-reads-genetic-code | 11:03 |
| CaptHindsight | was looking back into this again | 11:04 |
| CaptHindsight | lots of projects like these get started and then stall | 11:04 |
| CaptHindsight | kanzure: did you ever get a reply from any biochemists about the sequencing? | 11:07 |
| kanzure | the sequencing? | 11:07 |
| kanzure | biochemists? | 11:08 |
| CaptHindsight | sorry, synthesis | 11:11 |
| kanzure | oh, chemists for synthesis | 11:13 |
| kanzure | got some replies but no bites | 11:13 |
| kanzure | need better ideas for looking | 11:13 |
| kanzure | not gonna find the right person on reddit etc | 11:14 |
| cpopell3 | kanzure : trying to get something synthesized? | 11:17 |
| kanzure | cpopell3: building synthesizer machin, want actual chemists to be involved | 11:20 |
| kanzure | *machine | 11:20 |
| cpopell3 | kanzure : biochemists or straight up chemists? | 11:22 |
| kanzure | chemists | 11:24 |
| kanzure | biochemists aren't skilled in this area | 11:24 |
| kanzure | and are usually disinterested | 11:24 |
| kanzure | cpopell3: http://diyhpl.us/wiki/dna/synthesis/notes/ | 11:25 |
| cpopell3 | kanzure : yeah, was just trying to think of who I know, but it's pretty much limited to Xander Honkala and Andre Watson | 11:25 |
| kanzure | well, asking them might be productive | 11:26 |
| kanzure | or if you have another strategy for finding a chemist that would be compatible working on an hplusroadmap-style project.... | 11:26 |
| kanzure | one suggestion that i haven't pursued yet is reachout to professors running labs or school employment offices and offer straight up job, setup company to matchfit various pattern stuff, but this project doesn't exactly match typical norms :-) | 11:26 |
| kanzure | cpopell3: https://www.reddit.com/r/chemistry/comments/3gx7kg/seeking_collaborators_for_a_selffunded/ | 11:27 |
| cpopell3 | kanzure : yeah, as usual I don't really have anything in the wet science area | 11:29 |
| kanzure | man how the shit don't we have any chemists | 11:29 |
| cpopell3 | chemists are anal and banal? :P | 11:30 |
| kanzure | shouldn't be a problem | 11:31 |
| kanzure | what's weird is that eleitl counts as an organic chemist | 11:31 |
| kanzure | 'cept he claims to be far too rusty. | 11:31 |
| kanzure | and preoccupied with snails and devops | 11:32 |
| cpopell3 | meh | 11:32 |
| cpopell3 | my friends group is going more and more esoteric software folks (though maybe not esoteric by your standards) | 11:33 |
| kanzure | cpopell3: i encourage you to prevent that actually.... software is never going to be your weakness, given your ability to engage with internet communities. | 11:34 |
| cpopell3 | kanzure : yeah, I need to find more hard science friends. I'll join a hackerspace when I move to seattle I guess. the dc ones are inconvenient | 11:35 |
| eudoxia | yeah, they're probably all fed plants | 11:37 |
| eudoxia | :p | 11:37 |
| CaptHindsight | everyone I've talked to involved with synthesis doesn't like to discuss details | 11:39 |
| kanzure | most of them probably don't know details | 11:39 |
| CaptHindsight | they just don't want to share for free | 11:39 |
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| cpopell3 | eudoxia : lol, I get along fine with feds | 11:41 |
| ryankarason | cpopell3: moving to seattle, eh? | 11:43 |
| cpopell3 | next year~ | 11:43 |
| ryankarason | i have a scientist friend there | 11:43 |
| ryankarason | and he likes to share. | 11:43 |
| cpopell3 | girlfriend wants to go work at MSFT | 11:43 |
| cpopell3 | I might do the same | 11:43 |
| kanzure | we could also just send someone from here to go apprentice as a chemist | 11:43 |
| cpopell3 | I nominate eudoxia | 11:43 |
| kanzure | fenn: don't you have family that does weird chemistry stuff | 11:44 |
| ryankarason | i'd like to apprentice as a chemist | 11:44 |
| ryankarason | soon i hope to have enough space to build a lab :) | 11:44 |
| eudoxia | go where, i didn't read the whole scrollback | 11:44 |
| kanzure | your first task is to find a mentor | 11:44 |
| cpopell3 | eudoxia : we need you to go become an alchemist so you can refine spiritual pills for our pursuit of enlightenment | 11:45 |
| ryankarason | aye would be nice. i'll probably have to resort to the net.. and mentors over IRC ;P | 11:45 |
| eudoxia | cpopell3: your pursuit, hold on a sec, i want in on the enlightenment | 11:47 |
| cpopell3 | eudoxia : too much wuxia reading lately | 11:48 |
| cpopell3 | eudoxia : start with one of these - http://www.mamakim.com/attachments/Image/loupan1.jpg | 11:48 |
| eudoxia | it looks like an ancient circular slide rule | 11:49 |
| cpopell3 | it's a feng shui compass, heh | 11:49 |
| cpopell3 | kanzure : do you have good resources on lambda calculus? my top rec so far is from eudoxia: ' ?_Common Lisp: A Gentle Introduction to Symbolic Computation_?' | 11:56 |
| kanzure | you don't need anything other than eudoxia | 12:01 |
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| FourFire | fenn, why is the copy of permutation city, which you host, incomplete? | 12:02 |
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| CaptHindsight | anyone here at Caltech? | 12:27 |
| kanzure | technically no but there's the la-biohackers in the area | 12:31 |
| juul | ~2600 igem attendees | 12:42 |
| kanzure | pants them all | 12:52 |
| kanzure | juul: see http://diyhpl.us/wiki/dna/projects/#igem-2015 and http://diyhpl.us/wiki/dna/projects/#igem-2014 | 12:52 |
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| juul | kanzure: nice :) | 13:56 |
| kanzure | juul: convince some igem people to work on these things http://groups.google.com/group/enzymaticsynthesis | 13:59 |
| juul | kanzure: good idea. i shall bring it up constantly | 14:00 |
| kanzure | or find the tdt dna synthesis team from last year | 14:00 |
| kanzure | excellent | 14:00 |
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| kanzure | "Someone made a VR theater for the oculus rift (it's on Steam) which allows you to stream a movie or video to watch with other friends" | 14:13 |
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| poppingtonic | Nice | 14:17 |
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| poppingtonic | similar to Netflix's app? http://techblog.netflix.com/2015/09/john-carmack-on-developing-netflix-app.html | 14:42 |
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| poppingtonic | cpopell3: eudoxia's recommendation is great. if you're totally new to Lisp you might also want to check out Land of Lisp by Conrad Barski, followed by On Lisp by Paul Graham. The Little Schemer is also really good. | 14:53 |
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| maaku | kanzure: I know a chem engineer I might be able to persuade into hplus stuff | 15:13 |
| maaku | he's currently preoccupied with space stuff | 15:13 |
| kanzure | maaku: well we're good at talking about space stuff, so that may not be too hard | 15:14 |
| kanzure | hmm there's only a few million patents. reading all of their titles shouldn't be too hard. | 15:45 |
| * maaku awaits kanzure's epic 'review of patents since 1790' slideshow | 15:52 | |
| kanzure | unfortunately such a feat would probably make me unemployable | 15:55 |
| kanzure | better stop at 20 years ago | 15:55 |
| kanzure | wait who wants employment anyway | 15:55 |
| maaku | nobody _wants_ employment ;) | 15:56 |
| kanzure | nsa was censoring "death ray" patents with the assistance of the uspto https://archive.org/stream/41770909080999#page/n1/mode/2up | 16:00 |
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| kanzure | yashgaroth: with all your copious free time maybe you can find me a chemist for dna synthesis project | 16:40 |
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| yashgaroth | freedom starts next week; what was wrong with forcing eudoxia to become a chemist? I see no downsides | 16:42 |
| fenn | if the whole thing had been a mistake, there was no way, now, to undo it | 16:45 |
| fenn | you just have to know that the ending to permutation city is out there, somewhere | 16:45 |
| kanzure | yashgaroth: primary problem is that eudoxia is a few thousand km from civilization | 16:46 |
| yashgaroth | can't you set up a front company to sponsor his h1b or something? also I know ~0 chemists but will check around, it seems like that's a major deficiency in the community | 16:47 |
| kanzure | yeah we should sketch out deficiencies more and then keep on top of them | 16:47 |
| kanzure | i know that nmz787 has been wanting optics people | 16:47 |
| kanzure | we have a sufficiently crazy inkjet person, which is nice | 16:49 |
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| CaptHindsight | http://pratt.duke.edu/news/new-chip-produces-dna-faster-less-expensively | 17:24 |
| CaptHindsight | “Using the chip-based system, we add an enzyme that can recognize when a base pair is not where it should be, cut the defect out, and reassemble the strand,” | 17:25 |
| CaptHindsight | http://www.rsc.org/images/loc/2013/PDFs/Papers/638_0077.pdf | 17:30 |
| CaptHindsight | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424320/ | 17:32 |
| CaptHindsight | instead of chemically cleaving oligonucleotides off the chip for gene assembly, we developed isothermal nicking and strand-displacement amplification reactions to amplify and release overlapping gene-construction oligonucleotides into the sealed wells. Then without switching buffer or hands-on manipulations, polymerase cycling assembly reaction takes place in the same cells to construct gene sequences, currently up to 1kb each | 17:33 |
| CaptHindsight | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424320/figure/F1/ | 17:37 |
| CaptHindsight | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579223/ by processing both conventional and microarray-derived DNA oligonucleotides in combination with a bead-based high throughput pyrosequencing platform, gaining a 500-fold error reduction for microarray oligonucleotides | 17:44 |
| CaptHindsight | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139991/ A Scalable Gene Synthesis Platform Using High-Fidelity DNA Microchips | 17:46 |
| CaptHindsight | We tested our platform by assembling 47 genes, including 42 challenging therapeutic antibody sequences, encoding a total of ~35 kilo-basepairs of DNA. These assemblies were performed from a complex background containing 13,000 oligonucleotides encoding ~2.5 megabases of DNA, which is at least 50 times larger than previously published attempts. | 17:46 |
| CaptHindsight | pretty much the same players every time, Agilent and Illumina | 17:50 |
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| CaptHindsight | ErrASE http://www.synthesis.cc/2010/02/the-errase-synthetic-gene-error-correction-kit-from-novici.html | 17:52 |
| CaptHindsight | http://www.novicibiotech.com/life-technologies-signs-exclusive-license-agreement-for-dna-error-correction-technology-from-novici-biotech/ | 17:53 |
| CaptHindsight | Life Technologies is now ThermoFisher and ErrASE doesn't seem to be available on the website | 17:56 |
| CaptHindsight | ah it changed names as well, https://www.thermofisher.com/order/catalog/product/A14972 | 17:57 |
| CaptHindsight | CorrectASE™ Enzyme | 17:57 |
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| kanzure | looks about right | 18:28 |
| CaptHindsight | worked at Duke! | 18:39 |
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