| --- Log opened Fri Oct 16 00:00:58 2015 | ||
| -!- ArturShaik [~ArturShai@37.218.180.151] has joined ##hplusroadmap | 00:02 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 00:28 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 00:36 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] | 01:06 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Quit: Lost terminal] | 01:21 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 01:22 | |
| -!- gene_hacker [~chatzilla@c-98-232-239-159.hsd1.or.comcast.net] has quit [Ping timeout: 240 seconds] | 01:25 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 01:25 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 01:32 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 01:33 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 01:45 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Quit: Leaving] | 01:54 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 01:58 | |
| -!- ArturShaik [~ArturShai@37.218.180.151] has quit [Ping timeout: 250 seconds] | 02:10 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 02:13 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 02:24 | |
| -!- fleshtheworld- [~fleshthew@2602:306:cf0f:4c20:bd25:3c07:dda9:65b7] has quit [Read error: Connection reset by peer] | 02:33 | |
| -!- TeMPOraL [~temporal@temporal.superuser.org.pl] has joined ##hplusroadmap | 02:34 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has joined ##hplusroadmap | 02:47 | |
| -!- ArturShaik [~ArturShai@37.218.180.151] has joined ##hplusroadmap | 02:48 | |
| JayDugger | xfwm4 | 02:50 |
|---|---|---|
| JayDugger | And that's just dandy. | 02:50 |
| -!- JayDugger [~jwdugger@108.19.186.58] has quit [Client Quit] | 02:50 | |
| -!- ArturShaik [~ArturShai@37.218.180.151] has quit [Ping timeout: 240 seconds] | 02:53 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has joined ##hplusroadmap | 02:54 | |
| JayDugger | Good morning, everyone. | 02:54 |
| -!- ArturShaik [~ArturShai@37.218.180.151] has joined ##hplusroadmap | 03:08 | |
| -!- drewbot [~cinch@ec2-174-129-175-27.compute-1.amazonaws.com] has quit [Remote host closed the connection] | 03:18 | |
| -!- drewbot [~cinch@ec2-23-22-166-19.compute-1.amazonaws.com] has joined ##hplusroadmap | 03:18 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 03:28 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has joined ##hplusroadmap | 03:31 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 03:31 | |
| -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-jarawxtjcgbrcwfj] has quit [Quit: Connection closed for inactivity] | 03:38 | |
| kanzure | hmph | 03:47 |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has quit [Read error: Connection reset by peer] | 03:47 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has joined ##hplusroadmap | 03:48 | |
| -!- Houshalter [~Houshalte@oh-71-50-56-224.dhcp.embarqhsd.net] has joined ##hplusroadmap | 04:00 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has quit [Quit: Leaving.] | 04:02 | |
| -!- strangewarp_ [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Ping timeout: 272 seconds] | 04:24 | |
| -!- ArturShaik [~ArturShai@37.218.180.151] has quit [Ping timeout: 240 seconds] | 04:25 | |
| @nmz787 | guess its bedtime now | 04:33 |
| @nmz787 | I shall leave you with this | 04:34 |
| @nmz787 | small script i just wrote to convert the kicad plot and drill files to the OSHpark extensions, and also ZIP them up: https://gist.github.com/nmz787/e76770c4617cf80819a0 | 04:34 |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has quit [Ping timeout: 256 seconds] | 04:34 | |
| -!- nmz787 [~nmz787@unaffiliated/nmz787] has quit [Quit: leaving] | 04:39 | |
| -!- nmz787 [~nmz787@unaffiliated/nmz787] has joined ##hplusroadmap | 04:39 | |
| -!- ArturShaik [~ArturShai@195.114.248.147] has joined ##hplusroadmap | 04:45 | |
| -!- sandeepkr [~sandeepkr@111.235.64.4] has joined ##hplusroadmap | 04:52 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has joined ##hplusroadmap | 04:54 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 05:22 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 05:34 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Ping timeout: 240 seconds] | 05:44 | |
| -!- FourFire [~FourFire@185.7.192.138] has joined ##hplusroadmap | 05:59 | |
| -!- Aurelius2 [~cpopell@209.48.69.2] has quit [Ping timeout: 240 seconds] | 06:05 | |
| -!- Aurelius [~cpopell@209.48.69.2] has joined ##hplusroadmap | 06:06 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has joined ##hplusroadmap | 06:14 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 06:43 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has quit [Remote host closed the connection] | 06:53 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has joined ##hplusroadmap | 06:54 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 06:55 | |
| -!- Aurelius2 [~cpopell@209.48.69.2] has joined ##hplusroadmap | 07:02 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has quit [Remote host closed the connection] | 07:02 | |
| -!- Aurelius [~cpopell@209.48.69.2] has quit [Ping timeout: 240 seconds] | 07:05 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has joined ##hplusroadmap | 07:05 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has quit [Client Quit] | 07:05 | |
| -!- FourFire [~FourFire@185.7.192.138] has quit [Ping timeout: 255 seconds] | 07:11 | |
| -!- eudoxia [~eudoxia@r167-56-0-90.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 07:40 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:64db:4257:540b:62c7] has joined ##hplusroadmap | 07:44 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has joined ##hplusroadmap | 07:47 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has quit [Client Quit] | 07:49 | |
| kanzure | nmz787: if you are going to stay up looking at datasheets you might as well use octopart, you know | 07:50 |
| -!- JayDugger [~jwdugger@108.19.186.58] has joined ##hplusroadmap | 08:03 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 08:06 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has quit [Client Quit] | 08:07 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-ucvosgnnpntxeodt] has joined ##hplusroadmap | 08:12 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 08:15 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 08:16 | |
| -!- ArturShaik [~ArturShai@195.114.248.147] has quit [Ping timeout: 256 seconds] | 08:23 | |
| -!- ArturShaik [~ArturShai@195.114.248.147] has joined ##hplusroadmap | 08:28 | |
| -!- ArturShaik [~ArturShai@195.114.248.147] has quit [Ping timeout: 240 seconds] | 08:34 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Quit: Reconnecting] | 08:36 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 08:37 | |
| -!- hehelleshin [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 244 seconds] | 09:03 | |
| -!- zadock [~outsider@81.180.210.219] has joined ##hplusroadmap | 09:05 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Quit: Reconnecting] | 09:06 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 09:06 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Quit: Reconnecting] | 09:13 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 09:14 | |
| -!- delinquentme [~delinquen@162-194-93-250.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap | 09:20 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Ping timeout: 240 seconds] | 09:32 | |
| -!- MasterPiece [~MasterPie@unaffiliated/masterpiece] has joined ##hplusroadmap | 09:34 | |
| -!- ArturShaik [~ArturShai@195.114.248.147] has joined ##hplusroadmap | 09:37 | |
| delinquentme | with DNA nanopores ... what makes it so that the trans-pore speed is variable?? | 09:38 |
| kanzure | i thought there was an electrophoretic effect being used? | 09:43 |
| delinquentme | yeah thats the typical method | 09:45 |
| delinquentme | but for some reason the speed varies | 09:45 |
| delinquentme | OH wait!? | 09:45 |
| kanzure | increase/decrease voltage | 09:45 |
| delinquentme | noooo it can't be just because they use shitty PSUs ?? | 09:45 |
| kanzure | they use the PSUs with the voltage potentiometer knob things | 09:46 |
| delinquentme | dude. If thats why theres that variance in the speed I'm going to start screaming | 09:46 |
| delinquentme | kanzure, I think I'm missing the next step in that though you're expecting me to make? | 09:46 |
| delinquentme | http://twoporeguys.com/technology.html | 09:47 |
| kanzure | nah i am just making stuff up, i have never looked at nanopore speed stuff | 09:47 |
| kanzure | i have previously assumed they were using a setup very similar to gel electrophoresis for controlling molecule movement rate through nanopore | 09:48 |
| kanzure | and not a nanopore-electrode-specific mechanism | 09:48 |
| -!- MasterPiece [~MasterPie@unaffiliated/masterpiece] has quit [Remote host closed the connection] | 09:48 | |
| delinquentme | kanzure, do you understand why I want to scream? | 09:49 |
| -!- eudoxia_ [~eudoxia@r167-56-133-158.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 09:50 | |
| kanzure | nope, variable control of dna translocation through nanopore seems like a useful tihng to have control of. | 09:50 |
| delinquentme | also the tech for that above website is novel | 09:50 |
| delinquentme | and addresses exactly that | 09:50 |
| delinquentme | ( what you just said ) | 09:50 |
| -!- eudoxia [~eudoxia@r167-56-0-90.dialup.adsl.anteldata.net.uy] has quit [Read error: Connection reset by peer] | 09:50 | |
| delinquentme | BUT I'd want to scream because if it IS because of shitty PSUs or some kind of uncontrolled oddball capacitance changes in the machine | 09:51 |
| delinquentme | that just means this is another fucking stupid thing which SOMEHOW we've overlooked | 09:51 |
| delinquentme | law. just saw a dude in a Quid hoodie | 09:51 |
| kanzure | where is juri_ | 09:52 |
| kanzure | tim schmidt wants to ask about aluminum extruder things | 09:52 |
| kanzure | just gonna link to this one for now i guess https://media.ccc.de/browse/congress/2014/31c3_-_6417_-_en_-_saal_g_-_201412271245_-_3d_casting_aluminum_-_julia_longtin.html#video | 09:53 |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 246 seconds] | 10:00 | |
| kanzure | selective breeding project for neural recording and stimulation: select for brains that have large-scale connectivity to an easily-accessible region, for both stimulation and recording. so instead of having to stick a probe deep into the brain, just something on the surface that just-so-happens to route to areas-of-interest in the brain. selection can be based on brain implant recording/stimulation signal-to-noise ratios and observed ... | 10:02 |
| kanzure | ... phenotype behavior, and selection can also be based on physically observing large-scale connectivity of the brain. | 10:03 |
| kanzure | might be a way to do that with fetal brain surgery to redirect and repurpose large fiber pathways that will be developing later anyway | 10:03 |
| -!- souljack [souljack@209.141.38.87] has quit [Remote host closed the connection] | 10:06 | |
| -!- delinquentme [~delinquen@162-194-93-250.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 264 seconds] | 10:07 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 10:08 | |
| -!- delinquentme [~delinquen@162-194-93-250.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap | 10:11 | |
| -!- thundara_ is now known as thundara | 10:14 | |
| delinquentme | possibly strand unwinding... could cause fluctuations in trans-pore speed | 10:22 |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:64db:4257:540b:62c7] has quit [Read error: Connection reset by peer] | 10:22 | |
| delinquentme | kanzure, | 10:24 |
| delinquentme | i need to finish dis patent. | 10:24 |
| delinquentme | nmz787, can I call on the hive mind once again? | 10:24 |
| -!- eudoxia_ [~eudoxia@r167-56-133-158.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 10:28 | |
| CaptHindsight | I guess the earlier nanopore sensors didn't have multiple detectors to average out any noise | 10:31 |
| CaptHindsight | or was the bandwidth of the sensors to low for the rate at which the DNA moved across them? | 10:33 |
| delinquentme | CaptHindsight, combinatorics of both of those | 10:33 |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-ucvosgnnpntxeodt] has quit [Quit: Connection closed for inactivity] | 10:33 | |
| delinquentme | physical deformation / stretching could also contribute | 10:33 |
| delinquentme | physical restraints of co-locaiton of those sensors | 10:33 |
| CaptHindsight | looks like hardly anyone is working on this | 10:35 |
| CaptHindsight | had big pharma poopooed the idea so much that everyones been distracted from wanting this? | 10:36 |
| CaptHindsight | or is it patents? | 10:37 |
| delinquentme | CaptHindsight, what area? | 10:38 |
| CaptHindsight | nanopore dna sequencers | 10:39 |
| CaptHindsight | delinquentme: ^ | 10:39 |
| delinquentme | oh nanopores are a hot area of research | 10:40 |
| delinquentme | oxfornanopore? | 10:40 |
| -!- Houshalter [~Houshalte@oh-71-50-56-224.dhcp.embarqhsd.net] has quit [Quit: Quit] | 10:47 | |
| kanzure | .title https://www.youtube.com/watch?v=u9BHRo2d8nw | 10:48 |
| yoleaux | Optogenetic pacemaker - YouTube | 10:48 |
| kanzure | .title http://advances.sciencemag.org/content/1/9/e1500639 | 10:49 |
| yoleaux | kanzure: Sorry, that command (.title) crashed. | 10:49 |
| kanzure | hmph | 10:49 |
| kanzure | "A general feedback theory of human behavior" http://www.amsciepub.com/doi/pdf/10.2466/pms.1960.11.1.71 | 10:50 |
| kanzure | no idea why i had that open in a tab | 10:50 |
| kanzure | "the spatial topography of the neocortex is strongly reflected in its molecular topography-the closer two cortical regions, the more similar their transcriptomes [266]" | 10:59 |
| kanzure | "Data from the Allen Mouse Atlas shows that specific genetic markers, such as RAR-related orphan receptor beta, potassium voltage-gated channel, subfamily H (eag-related) member 7, ephrin A5, and activity regulated cytoskeletal-associated protein (Arc) are expressed at markedly higher levels in primary sensory areas (V1, S1, and A1) than elsewhere in the cortex (Stefan Mihalas, personal communication)." | 11:00 |
| -!- zadock [~outsider@81.180.210.219] has quit [Quit: Leaving] | 11:01 | |
| kanzure | hm he wants glucose-powered brain implants as an alternative to wireless energy transmission | 11:10 |
| kanzure | "An important caveat to these conclusions on wireless data transfer occurs if we consider the use of ultrasound rather than electromagnetic radiation. Because the speed of sound is dramatically slower than that of light, the wavelength of 10 MHz ultrasound is only ~150 microns (approximating the speed of sound in brain as the speed of sound in water, ~1500 m/s). Thus, many 10 MHz ultrasound transmitters/receiver could be placed inside a ... | 11:15 |
| kanzure | ... mouse brain while maintaining their spatial separation above the wavelength, and a linear scaling of the MIMO channel capacity with the number of devices is likely possible in this regime, assuming the appropriate antenna gains and orientations can be achieved inside brain tissue. Beam orientation could present a challenge if micro-devices are oriented randomly after implantation. With an attenuation of 0.5 dB/(cm MHz)[6], the ... | 11:15 |
| kanzure | ... attenuation at 10 MHz is only 5 dB/cm. Thus ultrasound-based transmission of power and data from embedded recording devices may be viable[585]." | 11:15 |
| kanzure | mri contrast agent based on neurotransmitter release[596] | 11:16 |
| kanzure | "Replication of the 3 billion bp human genome takes approximately 8 hours in normally dividing cells, which is a nucleotide incorporation rate of ~100 kHz; in order to not exceed the metabolic rates associated with normal genome replication, molecular ticker tapes operating at 1 kHz polymerization speed[343] would be limited to approximately 100 simultaneously replicated templates per cell. Equivalently, ~1700 RNA tickertapes each ... | 11:19 |
| kanzure | ... operating at 1 kHz." | 11:19 |
| kanzure | "The maximal rate of neuronal aerobic respiration is ~5 fmol of ATP minute via oxidative respiration. Assuming ~1 ATP equivalent consumed per nucleotide incorporation, if neuronal metabolism were entirely dedicated to polymerization, it would support the incorporation of up to 6e9 nucleotides per minute, or 10^5 simultaneously replicated DNA templates at 1 kHz." | 11:20 |
| kanzure | "Ticker tapes operating at 1 kHz with 10,000 simultaneously replicated templates could record for 300 seconds before the total length of DNA synthesized equals the human genome length. In the case of RNA polymerase 2-based transcription, 2.75h of recording by 10,000 recorders is required to reach the net transcript length in the cell." | 11:21 |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-ybdjewzzkneqneay] has joined ##hplusroadmap | 11:23 | |
| -!- delinquentme [~delinquen@162-194-93-250.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 260 seconds] | 11:28 | |
| -!- nmz787_i [~ntmccork@192.55.54.43] has joined ##hplusroadmap | 11:28 | |
| -!- sandeepkr [~sandeepkr@111.235.64.4] has quit [Remote host closed the connection] | 11:31 | |
| -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has quit [Ping timeout: 256 seconds] | 11:34 | |
| -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has joined ##hplusroadmap | 11:36 | |
| nmz787_i | kanzure: wasn't looking at datasheets last night, was laying out a pcb | 11:37 |
| -!- nmz787_i [~ntmccork@192.55.54.43] has quit [Quit: Leaving.] | 11:38 | |
| -!- delinquentme [~delinquen@209.60.252.90] has joined ##hplusroadmap | 11:38 | |
| -!- delinquentme [~delinquen@209.60.252.90] has quit [Ping timeout: 240 seconds] | 11:49 | |
| -!- MasterPiece [~MasterPie@unaffiliated/masterpiece] has joined ##hplusroadmap | 11:56 | |
| -!- MasterPiece [~MasterPie@unaffiliated/masterpiece] has quit [Remote host closed the connection] | 12:02 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 12:03 | |
| -!- MasterPiece [~MasterPie@unaffiliated/masterpiece] has joined ##hplusroadmap | 12:03 | |
| -!- ArturShaik [~ArturShai@195.114.248.147] has quit [Ping timeout: 240 seconds] | 12:10 | |
| -!- MasterPiece [~MasterPie@unaffiliated/masterpiece] has quit [Quit: Leaving] | 12:17 | |
| -!- helleshin [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 12:28 | |
| -!- nmz787_i [~ntmccork@192.55.55.41] has joined ##hplusroadmap | 12:30 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Ping timeout: 265 seconds] | 12:35 | |
| nmz787_i | .tell delinquentme what do you request of the hive-mind? | 12:36 |
| yoleaux | nmz787_i: I'll pass your message to delinquentme. | 12:36 |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] | 12:36 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has joined ##hplusroadmap | 12:36 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 12:37 | |
| nmz787_i | .tell delinquentme: i bet the variation is simply due to each base having a different electronic configuration. It would be quite a lapse in engineering if they would allow the design to have sensors with enough amplification and low enough noise not to notice a crappy rippling powersupply. And in fact, I would bet that the powersupply ripple would massively affect the sensor readings, so I have real doubts about that being the case. | 12:37 |
| yoleaux | nmz787_i: What kind of a name is "delinquentme:"?! | 12:37 |
| nmz787_i | .tell delinquentme : i bet the variation is simply due to each base having a different electronic configuration. It would be quite a lapse in engineering if they would allow the design to have sensors with enough amplification and low enough noise not to notice a crappy rippling powersupply. And in fact, I would bet that the powersupply ripple would massively affect the sensor readings, so I have real doubts about that being the case. | 12:38 |
| yoleaux | nmz787_i: I'll pass your message to delinquentme. | 12:38 |
| nmz787_i | hmm, https://www-ssl.intel.com/content/www/us/en/do-it-yourself/arduino-101.html | 12:42 |
| nmz787_i | .title | 12:42 |
| yoleaux | Arduino 101* Board | Learn the Basics and Become a Maker | 12:42 |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Ping timeout: 256 seconds] | 12:45 | |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] | 12:45 | |
| kanzure | https://medium.com/@bramcohen/how-wallets-can-handle-transaction-fees-ff5d020d14fb | 12:47 |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 13:11 | |
| -!- nmz787_i1 [~ntmccork@192.55.54.38] has joined ##hplusroadmap | 13:22 | |
| nmz787_i1 | delinquentme: see logs for some responses to you | 13:22 |
| delinquentme | kk | 13:23 |
| yoleaux | 19:36Z <nmz787_i> delinquentme: what do you request of the hive-mind? | 13:23 |
| yoleaux | 19:38Z <nmz787_i> delinquentme: : i bet the variation is simply due to each base having a different electronic configuration. It would be quite a lapse in engineering if they would allow the design to have sensors with enough amplification and low enough noise not to notice a crappy rippling powersupply. And in fact, I would bet that the powersupply ripple would massively affect the sensor readings, so I have real doubts about that | 13:23 |
| yoleaux | being the case. | 13:23 |
| -!- nmz787_i [~ntmccork@192.55.55.41] has quit [Ping timeout: 260 seconds] | 13:24 | |
| -!- Houshalter [~Houshalte@oh-71-50-56-224.dhcp.embarqhsd.net] has joined ##hplusroadmap | 13:45 | |
| -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-vignjcswtfkbgxvw] has joined ##hplusroadmap | 13:46 | |
| -!- nmz787_i [~ntmccork@192.55.55.41] has joined ##hplusroadmap | 14:01 | |
| -!- nmz787_i1 [~ntmccork@192.55.54.38] has quit [Remote host closed the connection] | 14:04 | |
| -!- Aurelius2 [~cpopell@209.48.69.2] has quit [Ping timeout: 272 seconds] | 14:06 | |
| kanzure | nsh: wikipedia's article on signal-noise ratio doesn't really elaborate about research about online communities. do you have anything? | 14:08 |
| kanzure | nsh: most of the conversation about signal-noise ratio in online discussions is often about merely very obvious noise, rather than robust signal that grows over time. instead we often just see signal diminishing over time. | 14:11 |
| kanzure | buy-an-account strategy is interesting if you could allow people to get past the paywall with nominations. (or really, probably need paywall + nominations and the nominations get to decide appropriate paywall level, which in some cases might be zero) | 14:14 |
| kanzure | i guess that might count as a pyramid scheme for content. oops. | 14:15 |
| delinquentme | nmz787_i, actually i was after the hive mind for how to get the positioning done for this DNA ligation | 14:18 |
| kanzure | dna ligase attaches to dna molecules on its own | 14:18 |
| delinquentme | I've suggested a number of ways to align the DNA with molecular-scale grooves in the alignment platform | 14:18 |
| delinquentme | targeted heating elements at the ligation site to increase local energy w intent of overcoming any minimum for ligation | 14:19 |
| delinquentme | samesies w forcues thz waves | 14:19 |
| delinquentme | focused* | 14:19 |
| delinquentme | kanzure, the mechanoligation | 14:19 |
| delinquentme | the afm / stm tip forced joining of dsDNA or ssDNA | 14:20 |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 246 seconds] | 14:20 | |
| kanzure | mechanical force can't cause ligation exceptinasmuch as it can cause dna molecules to be positioned close enough for a chemical reaction to occur | 14:20 |
| delinquentme | also included the CNT threading nmz787 suggested | 14:20 |
| delinquentme | why not? | 14:20 |
| delinquentme | everything in the research says its quite possible | 14:20 |
| kanzure | not like smashing two potatoes together | 14:20 |
| delinquentme | including research from Freits and Merkle | 14:21 |
| delinquentme | they're doing it for diamonds | 14:21 |
| delinquentme | and unless im incorrect ( im not ) those are much more energetic bonds than DNA | 14:21 |
| kanzure | yes they were using covalent bond chemistry | 14:22 |
| delinquentme | but define "chemistry" here | 14:22 |
| delinquentme | they're simply doing deposition | 14:22 |
| delinquentme | well characterized AFM tip deposition of carbon atoms | 14:22 |
| delinquentme | oh wait sorry | 14:23 |
| delinquentme | n 2003, Oyabu et al.[17] reported the first instance of purely mechanical-based covalent bond-making and bond-breaking, i.e., the first experimental demonstration of true mechanosynthesis—albeit with silicon rather than carbon atoms. | 14:23 |
| delinquentme | thats like the thing that i keep hearing from chemists | 14:27 |
| delinquentme | like chemistry is magic | 14:27 |
| delinquentme | nah its just overcoming local energy fields | 14:27 |
| kanzure | technically, chemistry is alchemy | 14:27 |
| kanzure | magic is something else | 14:27 |
| delinquentme | whats a rather large stationary molecule which would affix to a set sequence on a strand of DNA? | 14:28 |
| kanzure | dna ligase, dna polymerase, etc. | 14:28 |
| delinquentme | stationary as in ... its not ratcheting its way down the DNA | 14:28 |
| delinquentme | like it will simply attach to a sequence and sit its ass there | 14:29 |
| kanzure | you can biotinylate a dna molecule at various bases | 14:29 |
| kanzure | or is that streptavidin. i can never remember this.... | 14:29 |
| delinquentme | im asking because if I can put a big fat molecule near the position I want to ligate at .. it would allow me more control over rotationial orientation of the dna | 14:30 |
| kanzure | you can also use aptamers or antibodies to bind to particular dna molecules | 14:30 |
| delinquentme | just because the thing im rotating is bigger | 14:30 |
| kanzure | you are not going to be able to rotate the entire dna molecule. it will just twist and turn. it's a giant piece of string. | 14:31 |
| delinquentme | yeah but all i need is rotation at the ligation site | 14:31 |
| delinquentme | phuck the rest of it | 14:31 |
| delinquentme | dat head. | 14:31 |
| delinquentme | sorry. | 14:31 |
| delinquentme | im reading research so i took some addies | 14:31 |
| kanzure | http://diyhpl.us/~bryan/papers2/DNA/ | 14:32 |
| kanzure | hmm i forgot about click chemistry dna ligation | 14:33 |
| delinquentme | silly question: when people are doing typical ligation of DNA | 14:36 |
| delinquentme | is ATP added in as a spearate chemical? | 14:36 |
| -!- nmz787_i1 [ntmccork@nat/intel/x-rdgohrwwvowomjcv] has joined ##hplusroadmap | 14:36 | |
| delinquentme | http://www.molecularassembler.com/Nanofactory/Challenges.htm | 14:37 |
| delinquentme | nmz787_i1, unsure if you saw the mentions above | 14:37 |
| -!- nmz787_i [~ntmccork@192.55.55.41] has quit [Ping timeout: 264 seconds] | 14:38 | |
| kanzure | t4 dna ligase reuires atp | 14:38 |
| delinquentme | work piece surfaces. | 14:39 |
| delinquentme | thats the term i've been looking for | 14:39 |
| delinquentme | kanzure, yeah. so where does it come from | 14:39 |
| delinquentme | its not in purified water that these reactions are run in | 14:39 |
| delinquentme | where comes from? | 14:39 |
| kanzure | probably the buffer mix | 14:40 |
| kanzure | https://www.neb.com/products/b0202-t4-dna-ligase-reaction-buffer | 14:40 |
| delinquentme | small black holes which spring in and out of existance at the interection of valence bonds of quantium tunneling magic alechmistry chem? | 14:40 |
| delinquentme | someone should kick my face in for that =[ | 14:40 |
| kanzure | tactical drone was deployed like 10 minutes ago, way ahead of you | 14:40 |
| delinquentme | this is why face to face is so good | 14:40 |
| delinquentme | i dont get aggro | 14:40 |
| delinquentme | hahahahaha | 14:41 |
| delinquentme | <3 | 14:41 |
| kanzure | unfortunately it missed and landed somewhere in iran, so ::shrug:: | 14:41 |
| delinquentme | fucking quantum covalence | 14:42 |
| kanzure | is Challenges.htm new to you? | 14:42 |
| delinquentme | shitting on physics based effects everywhere | 14:42 |
| delinquentme | yeaaaaa | 14:42 |
| delinquentme | reading it for first tiem nao | 14:42 |
| kanzure | have you read the tooltip paper? http://diyhpl.us/~bryan/papers2/nanotech/Optimal%20tooltip%20trajectories%20in%20a%20hydrogen%20abstraction%20tool%20recharge%20reaction%20sequence%20for%20positionally%20controlled%20diamond%20mechanosynthesis.pdf | 14:43 |
| delinquentme | nopeeee | 14:46 |
| delinquentme | trying to download some paper and my stupid stupid thing is being stupid | 14:46 |
| delinquentme | Mechanical Vertical Manipulation of Selected Single Atoms by Soft Nanoindentation | 14:49 |
| delinquentme | Using Near Contact Atomic Force Microscopy | 14:49 |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has joined ##hplusroadmap | 14:51 | |
| -!- Houshalter [~Houshalte@oh-71-50-56-224.dhcp.embarqhsd.net] has quit [Quit: Quit] | 14:53 | |
| delinquentme | im writing a patent =| | 14:53 |
| delinquentme | im like | 14:53 |
| delinquentme | whats then word when you cant talk to a really cute girl? | 14:54 |
| delinquentme | yeah. dat feel | 14:54 |
| delinquentme | lol fucking wat. | 14:56 |
| delinquentme | dude. what if we just have mechanically constrained ends of DNA with ligase molecules or whatever smashed into a substrate ( nano imporint lithography or whateves ) | 14:56 |
| maaku | delinquentme: you are patenting atomic mechanosynthesis using an AFM? | 14:56 |
| delinquentme | then just let the ends we want to ligate hang free | 14:57 |
| delinquentme | STM | 14:57 |
| delinquentme | =| | 14:57 |
| delinquentme | maaku plz tear it apart | 14:57 |
| maaku | uh, (1) prior art | 14:57 |
| maaku | (2) dear god why? | 14:57 |
| kanzure | maaku: i suspect that he believes writing and getting a patent will help him with raising funding in the future. but that's my only guess. | 14:59 |
| maaku | has he done anything novel? | 14:59 |
| delinquentme | prior art on mechanical synthesis for DNA ligation? | 14:59 |
| delinquentme | plz provide | 15:00 |
| maaku | delinquentme: have you actually accomplished this? | 15:00 |
| delinquentme | maaku no | 15:01 |
| delinquentme | but we are building the STMs which seem like the tool to be used for this | 15:01 |
| delinquentme | mecanics / dampening are all compelte | 15:01 |
| delinquentme | complete* | 15:01 |
| delinquentme | and I have the eagle / something other designer PCB files | 15:02 |
| delinquentme | my EE/ roboticist is MIA at current | 15:02 |
| delinquentme | so im like OKAAAY what should I do in his absence ....? | 15:02 |
| maaku | delinquentme: let me get this straight: you are farming ##hplusroadmap for ideas for the purpose of filing a patent? | 15:02 |
| delinquentme | maaku im farming the ##hplusroadmap for ideas on how to position / align the DNA fragments | 15:03 |
| delinquentme | for the purpose of writing this into a patent | 15:03 |
| delinquentme | yes | 15:03 |
| maaku | methinks the proper response would be "fuck off" | 15:03 |
| maaku | and if that's out of line, then I'm outa here | 15:03 |
| delinquentme | maaku lol | 15:04 |
| delinquentme | why? | 15:04 |
| delinquentme | I think you might understimate how much value i put in you guys? | 15:04 |
| delinquentme | I talk this channel up so much. | 15:04 |
| delinquentme | IRL too. | 15:04 |
| maaku | so you want to take the ideas of people here and get an enforced monopoly on their use? | 15:04 |
| maaku | do you really not see what is wrong about that? | 15:05 |
| delinquentme | maaku, i think you dont understand how much value I put in this channel. | 15:07 |
| maaku | delinquentme: so patent your own fucking ideas | 15:08 |
| delinquentme | maaku stated more directly: if anything came of this i'd be pulling in people from this channel. | 15:08 |
| delinquentme | ok well i appreciate your input | 15:08 |
| delinquentme | thanks. | 15:08 |
| -!- maaku [~quassel@botbot.xen.prgmr.com] has left ##hplusroadmap ["http://quassel-irc.org - Chat comfortably. Anywhere."] | 15:08 | |
| delinquentme | kanzure, am I being out of line here? | 15:10 |
| delinquentme | I mean more so than what is acceptable? | 15:10 |
| kanzure | "Chemical transformation of human astroglial cells into neurons for brain repair" http://www.cell.com/cell-stem-cell/abstract/S1934-5909(15)00419-1 | 15:11 |
| kanzure | "Small Molecules Efficiently Reprogram Human Astroglial Cells into Functional Neurons" | 15:11 |
| kanzure | i think that it's fair to say that intellectual property law is severely fucked up at the moment; there's no way to know whether you can successfully stop patent trolls from litigating the hell out of you even if you have prior art (you still have to pay the expenses of defending against the patent troll). | 15:13 |
| kanzure | it's a really murky question; what some companies do is they just "promise" to not litigate the shit out of everyone with their patent portfolio. but promises ain't worth nothing. | 15:14 |
| kanzure | the idea of taking ideas from a public community and then patenting them is definitely a little unethical; but that's the entire premise of the whole patent system. | 15:16 |
| kanzure | i am not sure if we should be excommunicating people just because they have to interact with the patent system. non-participation is not always optimal. | 15:16 |
| kanzure | but also... i would guess that you are overestimating the utility of filing for patents. | 15:17 |
| -!- poppingtonic [~Thunderbi@unaffiliated/poppingtonic] has quit [Read error: Connection reset by peer] | 15:20 | |
| delinquentme | Id agree w everything thats been said | 15:23 |
| delinquentme | but I want to empahsize that I see ##hplus as the brain trust. | 15:23 |
| delinquentme | but I think we all already know this? | 15:23 |
| delinquentme | maybe maaku doesnt idk. | 15:23 |
| kanzure | whatis brain trust? | 15:25 |
| -!- nmz787_i1 [ntmccork@nat/intel/x-rdgohrwwvowomjcv] has quit [Quit: Leaving.] | 15:25 | |
| kanzure | http://journals.lww.com/plasreconsurg/Fulltext/2015/10000/Nerve_Transfers_to_Restore_Upper_Extremity.24.aspx | 15:26 |
| delinquentme | as in like the think tank of applied transhumanisms and all things " INTERESTING ENGINEERING" | 15:26 |
| kanzure | cool i should reroute some of my leg nerves to my hands, i don't need fine motor control of my feet do i | 15:26 |
| delinquentme | and the first people I'd hire to work with me | 15:26 |
| delinquentme | because I know you guys | 15:26 |
| kanzure | http://diyhpl.us/wiki/hplusroadmap | 15:26 |
| delinquentme | even if I can afford some of the oldfags | 15:26 |
| delinquentme | <3 | 15:27 |
| kanzure | "In spinal cord injury, a nerve transfer bypasses the damaged spinal cord area to deliver a signal from the brain to a muscle that became disconnected following that injury. A donor nerve is taken from a nonessential uninjured muscle and transferred to provide a more critical function (Fig. 1). Because the nerve transfer procedure involves cutting and reattaching peripheral nerves, time is required to reinnervate and to allow the brain ... | 15:30 |
| kanzure | ... to relearn how to use that muscle. Individual patient outcomes are subtle and variable and can take months to 1 year to measure; however, they can dramatically improve function and independence." | 15:31 |
| kanzure | huh why do they have to cut out the whole nerve. that seems less good. | 15:31 |
| -!- Burninate [~Burn@pool-74-96-98-64.washdc.fios.verizon.net] has quit [Read error: Connection reset by peer] | 15:36 | |
| TMA | from what I recall from anatomy, the periferial nerves are very thin, -- cutting them lengthwise to obtain a fiber is beyond the ability of the surgeon | 15:36 |
| -!- Burninate [~Burn@pool-74-96-98-64.washdc.fios.verizon.net] has joined ##hplusroadmap | 15:37 | |
| TMA | surgery is very imprecise in general :( | 15:39 |
| mgin | can anyone explain this shit??? http://www.rifters.com/crawl/?p=6116 | 15:39 |
| mgin | "one out of ten hydrocephalus cases are so extreme that cerebrospinal fluid fills 95% of the cranium. Anyone whose brain fits into the remaining 5% should be nothing short of vegetative; yet apparently, fully half have IQs over 100." | 15:40 |
| mgin | what the fuck?? | 15:40 |
| mgin | is this a hoax? | 15:40 |
| archels | no, it's real | 15:42 |
| archels | there might be deficiencies that are conveniently glanced over in the anectodal reports though | 15:42 |
| archels | but in general, if things go wrong at an early enough ontological stage, the cortex is pretty resilient | 15:43 |
| mgin | but i mean, it has to be false that a person could be totally normal, above average IQ, with only 5% of their brain matter? | 15:43 |
| mgin | bizarre | 15:44 |
| kanzure | amount of brain matter may not influence cognitive ability | 15:44 |
| kanzure | iq was not created to measure brain volume | 15:45 |
| mgin | implies that the calculations of ops/second required for a functional intelligence may be wildly overstated | 15:45 |
| -!- drethelin [drethelin@24-241-226-112.dhcp.mdsn.wi.charter.com] has quit [Ping timeout: 252 seconds] | 15:45 | |
| mgin | ugh | 15:45 |
| delinquentme | AV-groove similar to groove 109 etched in silicon using KOH inWaterastheetchanthasbeenshoWnbytransmission electron microscopy (TEM) to have “a rounded shape that has a radius of curvature of around 3 nm” | 15:45 |
| delinquentme | any idea what KOH might be here? | 15:46 |
| kanzure | same KOH as in semiconductor manufacturing | 15:46 |
| kanzure | http://diyhpl.us/wiki/homecmos/wet-etch-recipes/ | 15:46 |
| -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap | 15:47 | |
| delinquentme | https://en.wikipedia.org/wiki/Potassium_hydroxide | 15:47 |
| kuudes | iirc there was at least one popularly referred hydrocephalus blog post that was in fact fake, I should check this though | 15:48 |
| kanzure | there was one claim about ct scanning not being sufficient, and that the brain mass was just hiding somewhere else | 15:48 |
| mgin | makes much more sense | 15:49 |
| mgin | doesn't past the stink test | 15:49 |
| mgin | pass* | 15:49 |
| kanzure | lotta people survive with half their brain missing | 15:49 |
| kanzure | perhaps some of that mass is just for redundancy | 15:51 |
| kanzure | or all of the regular neurons are still there, just more squished together and maybe slightly smaller or something | 15:52 |
| -!- nmz787_i [~ntmccork@192.55.54.38] has joined ##hplusroadmap | 15:52 | |
| delinquentme | kanzure, you linked the paper on tip recharging for diamond mechanosynthesis | 15:59 |
| delinquentme | can I get a summary on why the tip needs to be recharged? | 16:00 |
| kanzure | elastography shows increase in brain stiffness in hydrocephalus patients http://www.fluidsbarrierscns.com/content/pdf/2045-8118-12-S1-O38.pdf | 16:03 |
| kanzure | delinquentme: probably electrons | 16:03 |
| -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has quit [Remote host closed the connection] | 16:13 | |
| -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has joined ##hplusroadmap | 16:14 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 16:18 | |
| delinquentme | what would be a method for loosly bonding the DNA backbone to something like an AFM tip ? | 16:41 |
| delinquentme | https://www.google.com/search?q=bonding+DNA+to+AFM+tip&ie=utf-8&oe=utf-8 | 16:41 |
| kanzure | biotin, streptavidin, aptamers, antibodies, click chemistry, polymerization, ... | 16:41 |
| kanzure | probably there's a surface tension method involving femtoliter amounts of water | 16:42 |
| delinquentme | http://www.molecularassembler.com/Papers/PDMM/Slide34.GIF | 16:50 |
| delinquentme | what does the rotation do here? | 16:50 |
| -!- Filipe [baf4fb64@gateway/web/freenode/ip.186.244.251.100] has joined ##hplusroadmap | 16:50 | |
| delinquentme | is this like particular molecules have lacking orbitals ... and by reorienting them you then get a change in the angular positions of particular bonds ... Thus letting it float free? | 16:51 |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-ybdjewzzkneqneay] has quit [Quit: Connection closed for inactivity] | 16:53 | |
| -!- Filipe [baf4fb64@gateway/web/freenode/ip.186.244.251.100] has left ##hplusroadmap [] | 16:54 | |
| delinquentme | surface potential cycling | 16:59 |
| -!- C0RVU5 [~C0RVU5@cpc7-hava2-2-0-cust1017.6-1.cable.virginm.net] has joined ##hplusroadmap | 17:22 | |
| -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Ping timeout: 252 seconds] | 17:26 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 17:30 | |
| nmz787_i | maaku_: I farm the whole world for ideas that I'm keeping a trade-secret... idk if that is better/worse/same as delinquentme-and-the-patent-gang | 17:35 |
| nmz787_i | delinquentme: if you want 'loose' coupling, just dry some DNA on the tip, it will stick... thanks to van der waals or whatever | 17:35 |
| delinquentme | ???? | 17:36 |
| delinquentme | example research? | 17:36 |
| delinquentme | nmz787_i, ty for defense . | 17:37 |
| nmz787_i | examples of what? drying DNA so it sticks? | 17:45 |
| nmz787_i | idk, crime scene investigation of dried semen is research??? | 17:45 |
| nmz787_i | but more relevant... would be drying DNA on gold nano/micro beads to be shot ballistically into cells (gene gun) | 17:46 |
| nmz787_i | delinquentme: http://diyhpl.us/~bryan/papers2/diybio/r%C3%BCdiger-trojok-gene-gun.pdf' | 17:47 |
| nmz787_i | http://diyhpl.us/~bryan/papers2/diybio/r%C3%BCdiger-trojok-gene-gun.pdf | 17:47 |
| nmz787_i | sorry I added a single-quote to the first link | 17:47 |
| nmz787_i | "A eukaryotic plasmid coding for a cyan fluorescent protein fused to a nuclear localisation sequence was loaded onto 1µm small gold particles, using Calcliumchloride and Spermidin (a chemical substance also found in mammal sperm). 10µl of the DNA-ladden gold particles in an alcoholic solution were spread on a 4cm² plastic foil.(See protocoll below). The alcohol evaporated and the gold tagged foil was curled up and loaded into the a piece of a | 17:48 |
| nmz787_i | rubber tube which then was stuck into Gene gun barrel (Pic.4), which was made from the tip of a pencil." | 17:48 |
| nmz787_i | delinquentme: also, mechanical synthesis will likely require significantly more energy than enzymatic or syn-chemistry | 17:49 |
| delinquentme | sure ... but why? | 17:50 |
| nmz787_i | its like pushing two magnets of same-polarity together and hoping they're strong enough to flip one or the other's field... so they stick instead of repele | 17:50 |
| delinquentme | I say " sure " because we have it in sheer mechanical actuator force | 17:50 |
| nmz787_i | with enzymes, the enzyme will reduce the opposition through side-effects elsewhere on the molecule | 17:50 |
| -!- drethelin [~drethelin@24-241-226-112.dhcp.mdsn.wi.charter.com] has joined ##hplusroadmap | 17:52 | |
| -!- drethelin [~drethelin@24-241-226-112.dhcp.mdsn.wi.charter.com] has quit [Client Quit] | 17:52 | |
| delinquentme | nmz787_i, but all of this is simply shifting electrons in and out of orbitals right? | 17:52 |
| nmz787_i | so if the enzyme touches the DNA a few atoms away from the ligation site, it will probably effect a pull on those electrons... which create vacancies, which the other electrons nearby will enjoy filling... and the whole point is that eventually electrons from the tip of the molecule are pulled away enough (or forced onto enough) to allow the 'snapping together' to kind of just happen (proximity of the molecules matter, as the 'magnetism' of the | 17:53 |
| nmz787_i | molecules can only travel so far before they won't attract each other) | 17:53 |
| nmz787_i | delinquentme: yeah | 17:53 |
| nmz787_i | it's kind of like picking a lock | 17:53 |
| nmz787_i | you have to pull some level on the backside of the molecule, to get the electrons to fall back, so the tip is vulnerable | 17:53 |
| nmz787_i | then once you violate the tip, you can put the electrons back and lock the tip down | 17:53 |
| nmz787_i | i'm sure we could cook up some sexual innuendos around this | 17:54 |
| delinquentme | im writing this verbatim into the patent | 17:55 |
| delinquentme | lol | 17:55 |
| delinquentme | for funsies. | 17:55 |
| nmz787_i | so the enzymes really are mechanicosynthesis... but they also tickle the electrons so less forcing together is needed | 17:55 |
| nmz787_i | you can probably liken this to temperature dependence of enzyme efficiency/througput/speed/product-flux | 17:57 |
| delinquentme | I feel like either were incredibly fucking wrong | 17:57 |
| delinquentme | or your like .. the first person who actually understands that . | 17:58 |
| delinquentme | yes. | 17:58 |
| delinquentme | also . there exist sates of DNA where one side has a severed backbone while the other side still has a contguous backboke | 17:59 |
| delinquentme | backbone* | 17:59 |
| nmz787_i | I think at least some of the molecular modelling/engineering people understand this | 17:59 |
| nmz787_i | aren't those called nicks? | 17:59 |
| delinquentme | bingo. | 18:02 |
| -!- drethelin [drethelin@24-241-226-112.dhcp.mdsn.wi.charter.com] has joined ##hplusroadmap | 18:05 | |
| nmz787_i | huh, this is so obvious, but I never considered it http://www.gizmag.com/lab-in-a-needle-houston-methodist/39708/ | 18:06 |
| nmz787_i | .title | 18:06 |
| yoleaux | "Lab in a needle" could streamline medical diagnoses | 18:06 |
| nmz787_i | .title http://pubs.rsc.org/en/Content/ArticleLanding/2015/LC/c5lc00798d | 18:07 |
| yoleaux | A lab-on-a-chip system integrating tissue sample preparation and multiplex RT-qPCR for gene expression analysis in point-of-care hepatotoxicity assessment - Lab on a Chip (RSC Publishing) | 18:07 |
| nmz787_i | hmm, wait a second, that doesn't mention -in-needle | 18:07 |
| nmz787_i | and what, they reference openPCR in the supplement | 18:07 |
| nmz787_i | http://www.rsc.org/suppdata/c5/lc/c5lc00798d/c5lc00798d1.pdf | 18:08 |
| nmz787_i | huh, and I found that link through something in my SPAM folder | 18:11 |
| nmz787_i | .title https://www.youtube.com/watch?v=3afEX8a2jPg | 18:12 |
| yoleaux | NASA | Jupiter in 4k Ultra HD - YouTube | 18:12 |
| -!- souljack [souljack@shell.xshellz.com] has joined ##hplusroadmap | 18:12 | |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has joined ##hplusroadmap | 18:15 | |
| -!- nmz787_i [~ntmccork@192.55.54.38] has quit [Ping timeout: 250 seconds] | 18:18 | |
| drethelin | did you guys see that alzheimer's fungal thing? | 18:18 |
| kanzure | nope | 18:31 |
| kanzure | .title https://news.ycombinator.com/item?id=10401344 | 18:35 |
| yoleaux | Different Brain Regions Are Infected with Fungi in Alzheimer’s Disease | Hacker News | 18:35 |
| -!- jaboja [~jaboja@erz89.neoplus.adsl.tpnet.pl] has joined ##hplusroadmap | 18:49 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:ccb3:43d1:d351:1df8] has joined ##hplusroadmap | 18:55 | |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has quit [Read error: Connection reset by peer] | 19:00 | |
| kanzure | howdy jaboja | 19:11 |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:ccb3:43d1:d351:1df8] has quit [Quit: Leaving] | 19:12 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 246 seconds] | 19:24 | |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:509f:ac2f:c4f:f1d1] has joined ##hplusroadmap | 19:31 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:9c60:c4f:e517:e1ec] has joined ##hplusroadmap | 19:33 | |
| -!- C0RVU5 [~C0RVU5@cpc7-hava2-2-0-cust1017.6-1.cable.virginm.net] has quit [Quit: Leaving] | 19:43 | |
| -!- strangewarp [~strangewa@50.141.118.234] has joined ##hplusroadmap | 19:46 | |
| -!- JayDugger [~jwdugger@108.19.186.58] has joined ##hplusroadmap | 20:25 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-hhbrkqkgtfmlunpk] has joined ##hplusroadmap | 20:42 | |
| kanzure | "Gene expression during zombie ant biting behavior reflects the complexity underlying fungal parasitic behavioral manipulation" http://www.biomedcentral.com/content/pdf/s12864-015-1812-x.pdf | 20:46 |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Read error: Connection reset by peer] | 21:00 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 21:06 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 21:10 | |
| delinquentme | https://en.wikipedia.org/wiki/Van_der_Waals_force | 21:39 |
| delinquentme | The resulting van der Waals forces can be attractive or repulsive.[2] | 21:39 |
| delinquentme | blah. I feel ignorant | 21:39 |
| JayDugger | Does "Andre Wegner" ring a bell? | 21:41 |
| JayDugger | Of "Authentise," a 3d printing shop out of Mountain View and NASA Ames. | 21:42 |
| delinquentme | nah | 21:43 |
| delinquentme | i dont know too many ppl at nasa though | 21:43 |
| delinquentme | 3 ... maaaybe? | 21:43 |
| JayDugger | Fair enough. Turns out I have a cousin who works at Authentise. | 21:43 |
| JayDugger | I'll probably see him before year's end, and it would be nice to have something to say other than "Hi! haven't seen you since your Dad's funeral." | 21:44 |
| JayDugger | My cousin's probably at their Utah office, come to think of it. | 21:45 |
| -!- jaboja [~jaboja@erz89.neoplus.adsl.tpnet.pl] has quit [Ping timeout: 250 seconds] | 21:53 | |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:509f:ac2f:c4f:f1d1] has quit [Quit: Leaving] | 22:18 | |
| -!- ArturShaik [~ArturShai@212.97.26.106] has joined ##hplusroadmap | 22:21 | |
| -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:9c60:c4f:e517:e1ec] has quit [Read error: Connection reset by peer] | 22:32 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 240 seconds] | 23:06 | |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 23:07 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 252 seconds] | 23:27 | |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 23:31 | |
| -!- gene_hacker [~chatzilla@c-98-232-239-159.hsd1.or.comcast.net] has joined ##hplusroadmap | 23:45 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 240 seconds] | 23:51 | |
| --- Log closed Sat Oct 17 00:00:09 2015 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!
