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| kanzure_ | .title https://www.youtube.com/watch?v=RXJKdh1KZ0w | 05:56 |
|---|---|---|
| yoleaux | Rockwell Retro Encabulator - YouTube | 05:56 |
| kanzure_ | .title https://www.youtube.com/watch?v=MXW0bx_Ooq4 | 06:00 |
| yoleaux | Chrysler Turbo Encabulator - YouTube | 06:00 |
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| kanzure_ | maaku: re: droplet array stuff, it occurs to me that it may not have been clear to you that there was a hydrophobic flat surface involved. that might help. | 06:21 |
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| kanzure | https://www.fightaging.org/archives/2015/11/progress-towards-engineered-extracellular-matrix.php | 07:49 |
| kanzure | https://www.fightaging.org/archives/2015/11/the-prospects-for-pig-to-human-xenotransplantation.php | 07:49 |
| kanzure | "United Therapeutics has spent $100 million in the past year to speed up the process of making transgenic pigs for lung transplants" | 07:49 |
| kanzure | http://cryonics-research.org.uk/ | 07:50 |
| kanzure | "A team led by Hal Sternberg of Biotime claimed to bring back to life baboons that were previously cooled to one degree Celsius. They injected a substance called Hextend to replace the blood and protect the cells from deterioration." | 07:51 |
| kanzure | "At a more basic level, Gregory Fahy and colleagues at 21st Century Medicine vitrified (i.e., stored at < -130°C without ice formation, see about page for details) a rabbit kidney and then transplanted it into a live animal with the kidney maintaining some degree of function. The same team showed that rat and rabbit brain slices can be vitrified and rewarmed and still maintain neural responses." | 07:51 |
| AmbulatoryC0rtex | growing spare organs on donor extracellular matrix and storing them cryogenically would be something | 07:58 |
| AmbulatoryC0rtex | keep a backup or two of all my organs | 07:58 |
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| AmbulatoryC0rtex | end up with a full set of organs grown when the guy was a month old or whatever | 08:06 |
| AmbulatoryC0rtex | to avoid age-related issues | 08:07 |
| kanzure | at the moment besides not knowing how to grow organs in that manner, the cryonics portion of your plan is also not going to work. | 08:07 |
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| AmbulatoryC0rtex | kanzure, I thought they did it with a heart? | 08:08 |
| AmbulatoryC0rtex | am I mistaken? | 08:08 |
| kanzure | did what? | 08:08 |
| AmbulatoryC0rtex | decellularized a pig heart, then regrew human tissue over the underlying extracellular matrix | 08:09 |
| kanzure | what does that have to do with your "grow organs and cryonically store them" plan? | 08:10 |
| AmbulatoryC0rtex | take cells from young person, and grow backup organs on decellularized pig organs | 08:11 |
| AmbulatoryC0rtex | then store for later, but you say that won't work | 08:11 |
| kanzure | growing tissue is not the same thing as growing organs | 08:11 |
| kanzure | yes, it's true that we don't have cryonics working for organs yet | 08:11 |
| AmbulatoryC0rtex | so you're saying the heart is actually easier than other organs, like the pancreas, for example | 08:12 |
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| kanzure | i have no idea how you think or what you're trying to communicate to me | 08:15 |
| kanzure | is this your attempt at making a sensationalized statement like "organs aren't real, only tissue" ? | 08:16 |
| AmbulatoryC0rtex | no, no | 08:16 |
| kanzure | if you can recreate the organs on demand, why would you need to cryonically store them? | 08:17 |
| AmbulatoryC0rtex | I'm suggesting stripping the cells out of a pig heart and growing human cells on the ECM using this process: http://www.nature.com/news/tissue-engineering-how-to-build-a-heart-1.13327 | 08:18 |
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| AmbulatoryC0rtex | the cryonics part is irrelevant if we can crogenically store organs | 08:18 |
| AmbulatoryC0rtex | can't* | 08:18 |
| kanzure | artificial organs swapped into another animal body would be useful if you wanted to control for ability to cryonically store the host, but that doesn't seem to be what you were saying | 08:18 |
| AmbulatoryC0rtex | and if we can't cryogenically store organs, the whole idea is moot | 08:19 |
| AmbulatoryC0rtex | the idea was to store young organs as a person ages | 08:19 |
| AmbulatoryC0rtex | by growing backups | 08:19 |
| AmbulatoryC0rtex | but it's moot, as we can't store them | 08:20 |
| kanzure | we could theoretically store them by freezing the cell lines or storing the cell lines in cryonic storage prior to organ construction iff (1) you know the exact cell lines required, (2) you have a working method of creating an organ with those cell lines once thawed. | 08:20 |
| kanzure | however, most of the cell lines can probably be constructed through direct differentiation of pluripotent stem cells, which can be constructed by dosing certain other cell lines (i'm not entirely convinced that older human cells are ungood for this, because ultimately eggs seem to survive for a few decades without debilitating mutation or aging). | 08:22 |
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| kanzure | when surgeons move a hand into the abdomen or something, does that require immunosuppression of any kind? | 08:25 |
| AmbulatoryC0rtex | no, as long as it's the patient's own tissue | 08:25 |
| AmbulatoryC0rtex | I'm trying to find a better articale than this nature one: http://www.nature.com/news/tissue-engineering-how-to-build-a-heart-1.13327 | 08:26 |
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| kanzure | hmm looks like decompressive craniectomy patients experience bone infection while their skull is stored in their abdomen | 08:31 |
| kanzure | "Replantation of Amputated Distal Phalangeal Parts of Fingers Without Vascular Anastomoses, Using Subcutaneous Pockets" http://journals.lww.com/plasreconsurg/Citation/1979/01000/Replantation_of_Amputated_Distal_Phalangeal_Parts.1.aspx | 08:32 |
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| kanzure | .title http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115275/ | 08:34 |
| yoleaux | Cranioplasty with subcutaneously preserved autologous bone grafts in abdominal wall—Experience with 75 cases in a post-war country Kosova | 08:34 |
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| kanzure | some images from a surgery to reconstruct forearm by storing it in the abdomen https://www.youtube.com/watch?v=bFBGuOxy5l0 | 08:38 |
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| pasky | https://en.wikipedia.org/wiki/Megaphragma_mymaripenne | 10:31 |
| chris_99 | intriguing | 10:32 |
| chris_99 | wonder if they sting | 10:32 |
| kanzure | .wik | 10:32 |
| yoleaux | Search for an article on Wikipedia | 10:32 |
| kanzure | .wik megaphragma mymaripenne | 10:32 |
| yoleaux | "Megaphragma mymaripenne is a microscopically sized wasp. At 200 μm in length, it is the third smallest extant insect, comparable in size to single celled organisms. It has a highly reduced nervous system, containing only 7400 neurons, several orders of magnitude fewer than in larger insects." — https://en.wikipedia.org/wiki/Megaphragma_mymaripenne | 10:32 |
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| chris_99 | .title http://www.sciencedaily.com/releases/2015/11/151109143616.htm | 11:26 |
| yoleaux | chris_99: Sorry, that doesn't appear to be an HTML page. | 11:26 |
| chris_99 | odd | 11:26 |
| chris_99 | 'Implantable wireless devices trigger, and may block, pain signals' | 11:26 |
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| kanzure | hmm. | 13:43 |
| kanzure | libpng buffer overflows https://news.ycombinator.com/item?id=10566664 | 13:51 |
| kanzure | comex commenting on libpng https://news.ycombinator.com/item?id=10567353 | 13:54 |
| chris_99 | nmz787, do you know what the actual lower end in nm of the CCD you used was out of interest, the graph just stops at 400nm on the datasheet iirc | 14:00 |
| chris_99 | ah http://oceanoptics.com/product/usb2000uv-vis/ since it seems to similar to the sony sensor in terms of sensitivity, i'll guess maybe it's also 200nm | 14:04 |
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| kanzure | "Meta-analysis of heritability of human traits based on 50 years of twin studies" http://www.gwern.net/docs/2015-polderman.pdf | 15:22 |
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| QuadIngi | kanzure, thoughts on this: https://www.indiegogo.com/projects/dracos-may-be-effective-against-all-viruses#/story | 15:49 |
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| FourFire | I'm mildly concerned that it will cause problems for a potential. future whole organism DNA editing vector, but then that's just a design problems | 15:51 |
| FourFire | DO DNA (as opposed to RNA) viruses exist (in nature?) | 15:51 |
| kanzure | DRACOS stuff in the logs would be good start | 15:56 |
| yashgaroth | there's plenty of dna viruses, but most viruses have an intermediate dsRNA stage in their lifecycle | 16:10 |
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| kanzure | hm | 19:57 |
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| kanzure | http://www.nycsubway.org/wiki/NXSYS,_Signalling_and_Interlocking_Simulator | 20:56 |
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| --- Log closed Sun Nov 15 00:00:25 2015 | ||
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