| --- Log opened Tue Jun 14 00:00:29 2016 | ||
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| archels | .title neuroinf.org | 02:45 |
|---|---|---|
| yoleaux | neuroinf.org | 02:45 |
| fenn | bad url? | 02:56 |
| archels | I think it's down, my email to comp-neuro bounced | 02:58 |
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| kanzure | hrm | 03:48 |
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| FourFire | .oed www.ncbi.nlm.nih.gov/pubmed/10028278 | 06:03 |
| yoleaux | FourFire: Sorry: that command is a web-service, but it didn't respond in plain text. | 06:03 |
| FourFire | kanzure, what's the correct command for that? | 06:03 |
| FourFire | .title www.ncbi.nlm.nih.gov/pubmed/10028278 | 06:18 |
| yoleaux | Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. - PubMed - NCBI | 06:18 |
| chris_99 | nmz787, i ordered the Pi Noir :) So I can try and build my spectrometer soon | 06:19 |
| mz_o__ | is that the ir camera for the raspi? | 07:00 |
| chris_99 | yeah | 07:00 |
| chris_99 | i found that apparently you can get raw output, with 10bit / channel interestingly | 07:00 |
| chris_99 | via the python api | 07:00 |
| mz_o__ | thats an interesting application for the pi. Seems like decent quality. Would resolution or bit depth be more important for a spectrometer? | 07:01 |
| chris_99 | for my application bit depth is pretty important, as i want to measure a peak around 900nm and measure the amplitude | 07:02 |
| chris_99 | i hope 10 bit is enough, but i'm not sure it will be | 07:03 |
| mz_o__ | Is there anything higher for a small camera? I konw with video files 10-bit is fairly high | 07:04 |
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| mz_o__ | Are you using any preexisting libraries for the actual spectrograph/analysis portion? | 07:04 |
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| chris_99 | i might just make them myself, i'm hoping it wonder be too hard | 07:06 |
| chris_99 | i'm trying to measure an ethanol peak | 07:06 |
| chris_99 | to determine ABV in a liquid | 07:06 |
| chris_99 | s/wonder/won't/ | 07:06 |
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| mz_o__ | It would really just be some basic image processing to analyze the peak on a certain area right? | 07:09 |
| kanzure | other half of zooko: https://github.com/ambimorph | 07:09 |
| chris_99 | mz_o__, yup afaik | 07:10 |
| mz_o__ | Where a group of pixels on a certain correspond to the wavelength | 07:10 |
| chris_99 | mm, and the pixel value corresponds to amplitude | 07:10 |
| mz_o__ | Im not to familiar with the spectrograph theory, YET | 07:10 |
| mz_o__ | just torrented a orgo lab manual with morning lol | 07:11 |
| mz_o__ | *this | 07:11 |
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| kanzure | hmph | 10:02 |
| nmz787_i | merphm? | 10:09 |
| kanzure | right | 10:11 |
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| kanzure | nmz787_i: we should just put him in irc instead. he's been in here before. | 10:16 |
| nmz787_i | .tell chris_99 I wonder if the camera has changed, I recall lots of digital noise on low light that I would consider good reading light... which I attributed to the layout of the camera module or something about how they did the analog to digital | 10:16 |
| yoleaux | nmz787_i: I'll pass your message to chris_99. | 10:16 |
| nmz787_i | hmm | 10:16 |
| chris_99 | nmz787_i, ah hmm, i think it's a sony sensor now, not sure if it was also before? | 10:17 |
| yoleaux | 17:16Z <nmz787_i> chris_99: I wonder if the camera has changed, I recall lots of digital noise on low light that I would consider good reading light... which I attributed to the layout of the camera module or something about how they did the analog to digital | 10:17 |
| nmz787_i | chris_99: can you read the name? | 10:17 |
| chris_99 | i haven't got it yet, but i think i can find the part no sec | 10:17 |
| nmz787_i | /me has mine in my hands | 10:17 |
| chris_99 | Sony IMX219 | 10:17 |
| nmz787_i | haha, my module has a cable that says 'SUNNY' | 10:18 |
| nmz787_i | raspberry pi camera rev 1.3 | 10:18 |
| chris_99 | sounds like the earlier version is Omnivision 5647 | 10:19 |
| chris_99 | so the noise could be different | 10:19 |
| chris_99 | i can always average it for my application though i guess | 10:20 |
| nmz787_i | yeah seems that is the right part you mentioned, from another random website confirming | 10:20 |
| chris_99 | aha | 10:20 |
| nmz787_i | in my case dark noise was really high | 10:20 |
| chris_99 | ah | 10:20 |
| nmz787_i | I'd expect better from Sony ;) | 10:20 |
| chris_99 | mm | 10:20 |
| nmz787_i | Omnivision doesn't have a playstation :) | 10:21 |
| chris_99 | heh | 10:21 |
| nmz787_i | at least | 10:21 |
| chris_99 | have you ever tried altering the focus of the lens? | 10:21 |
| nmz787_i | na | 10:22 |
| nmz787_i | used it as a security system one vacation, had an android app that could see it with a single click on my phone | 10:22 |
| nmz787_i | then it got disassembled and the OS started acting up or something | 10:22 |
| chris_99 | ah darn | 10:22 |
| nmz787_i | basically didn't spend enough time playing and messing with it | 10:23 |
| chris_99 | i'm gonna use the python api, were you can get raw pre-bayerised data | 10:25 |
| nmz787_i | kanzure: what about jcline coming in here? | 10:25 |
| nmz787_i | nice | 10:25 |
| nmz787_i | well | 10:25 |
| nmz787_i | no | 10:25 |
| nmz787_i | wait | 10:25 |
| nmz787_i | isn't it just pre-chopped to 8 bits? | 10:25 |
| kanzure | jcline wont do that | 10:26 |
| chris_99 | you can get 10 bit raw data via the python api | 10:26 |
| nmz787_i | yeah but that isn't pre-bayer | 10:26 |
| chris_99 | it is | 10:26 |
| nmz787_i | bayer is a layer of plastic on the silicon | 10:26 |
| chris_99 | sorry i mean the bayer algorithm where it creates pixels | 10:26 |
| nmz787_i | maybe you mean pre de-bayering | 10:26 |
| chris_99 | yeah | 10:26 |
| nmz787_i | ah, ok | 10:26 |
| nmz787_i | sorry | 10:26 |
| nmz787_i | physics vs algorithm | 10:26 |
| nmz787_i | overloaded | 10:26 |
| nmz787_i | just last night at work I was confused similarly over overloaded terms | 10:27 |
| nmz787_i | for totally disparate things | 10:27 |
| chris_99 | ah sorry i poorly explained that | 10:27 |
| nmz787_i | haha, na | 10:27 |
| chris_99 | you know this electroporation thing, you mentioned about inductors, i thought it uses a flyback to increase the voltagae | 10:28 |
| nmz787_i | yeah | 10:28 |
| nmz787_i | the one we want to get worked on | 10:28 |
| chris_99 | ahh, does that make it similar to how an SMPS works then, if you use inductors? | 10:29 |
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| kanzure | http://blogs.sciencemag.org/pipeline/archives/2016/06/10/crap-courtesy-of-a-major-scientific-publisher | 10:39 |
| kanzure | "Maybe what is needed is a “tiger team” approach to validating editorial and peer review. That is, papers should be concocted and submitted which contain clearly detectable errors or falsifications, with the intent of testing the editors and reviewers. If they don’t spot the bogosity and approve the paper, they get dinged. Multiple dings, and the ding score gets published. If the ding score goes high enough, the publishers or ... | 10:43 |
| kanzure | ... reviewers lose credentials." | 10:43 |
| kanzure | peerj and other startups would probably fund that | 10:52 |
| kanzure | but they would need to have a credible alternative to that system of peer review (e.g. can't be "just the same thing, this time with different overlords") | 10:52 |
| pasky | one issue is that it raises the cost of being a reviewer, you suddenly have a risk there; and it's largely altruistic activity (you have some incentive to learn some hot stuff ahead of the curve, but usually it's no hot stuff) | 10:58 |
| kanzure | making it altruistic-only is probably a mistake | 10:59 |
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| kanzure | "The first successful treatment of malignant cutaneous tumors implanted in mice was completed in 2007 by a group of scientists who achieved complete tumor ablation in 12 out of 13 mice. They accomplished this by sending 80 pulses of 100 microseconds at 0.3 Hz with an electrical field magnitude of 2500 V/cm to treat the cutaneous tumors.[5]" | 12:53 |
| kanzure | wikipedia article on electroporation does not say whether AC is a requirement, or what sort of wave shapes to bother with | 12:55 |
| kanzure | "Electroporation is a multi-step process with several distinct phases.[15] First, a short electrical pulse must be applied. Typical parameters would be 300–400 mV for < 1 ms across the membrane (note- the voltages used in cell experiments are typically much larger because they are being applied across large distances to the bulk solution so the resulting field across the actual membrane is only a small fraction of the applied bias). ... | 12:56 |
| kanzure | ... Upon application of this potential the membrane charges like a capacitor through the migration of ions from the surrounding solution. Once the critical field is achieved there is a rapid localized rearrangement in lipid morphology. The resulting structure is believed to be a “pre-pore” since it is not electrically conductive but leads rapidly to the creation of a conductive pore.[16] Evidence for the existence of such pre-pores ... | 12:56 |
| kanzure | ... comes mostly from the “flickering” of pores, which suggests a transition between conductive and insulating states.[17] It has been suggested that these pre-pores are small (~3 Å) hydrophobic defects. If this theory is correct, then the transition to a conductive state could be explained by a rearrangement at the pore edge, in which the lipid heads fold over to create a hydrophilic interface. Finally, these conductive pores can ... | 12:56 |
| kanzure | ... either heal, resealing the bilayer or expand, eventually rupturing it. The resultant fate depends on whether the critical defect size was exceeded[18] which in turn depends on the applied field, local mechanical stress and bilayer edge energy." | 12:56 |
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| kanzure | andrew suggests naming it "rainmaker" | 17:32 |
| kanzure | "because when it rains, it pores" | 17:32 |
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| nmz787_i | hmm, not bad | 17:37 |
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| fenn | bad | 17:47 |
| kanzure | i am not entirely sure how strong the science is regarding pore creation, that chunk of wikipedia text is not encouraging | 17:48 |
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| kanzure | "Instead of piezo starter, I am eager to have someone look at my HV power supply which can get up to 1800 VDC open circuit. (A cuvette is an open circuit.) The diy power supply plans are in the latest issue of BioCoder just out." | 20:56 |
| kanzure | oh i wonder if nobody has used that yet. maybe that's the issue. | 20:56 |
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| nmz787_i | why does he want someone to use that? | 21:20 |
| nmz787_i | I mean, it's nice for a reference, but it isn't ideal for this application I think, since we want small size and pulses via coils allow size reduction | 21:20 |
| nmz787_i | and when did anyone recently mention piezo??? | 21:22 |
| justanotheruser | someone say piezo? | 21:25 |
| nmz787_i | more like paleasssee-o | 21:26 |
| justanotheruser | where do I get a low piezoelectric coefficient piezo tube now-a-days | 21:26 |
| justanotheruser | sensor technology seems to not want to sell them in single units | 21:29 |
| nmz787_i | hmm, never heard of them before, makes sense though, the tube would give you more movement dynamic range right? | 21:30 |
| justanotheruser | more than what | 21:30 |
| nmz787_i | I wonder if you could use thermal expansion anisotropy | 21:30 |
| nmz787_i | mroe than a thin piezo disc | 21:31 |
| nmz787_i | more* | 21:31 |
| nmz787_i | like a tube with nichrome running axially | 21:31 |
| justanotheruser | I'm not sure. | 21:32 |
| justanotheruser | If the disks can be made with the same precision, I don't mind getting a disk | 21:32 |
| nmz787_i | well you would get less flex, I think | 21:36 |
| nmz787_i | if that is the case, look for a $1 or so buzzer | 21:36 |
| nmz787_i | no idea on the precision | 21:36 |
| nmz787_i | I'd guess it would have more imprecision between buzzers, than within a single unit | 21:37 |
| nmz787_i | but again, I have no idea | 21:37 |
| justanotheruser | the buzzers are buzzers because they don't require much electricity to move | 21:37 |
| justanotheruser | to get the same precision, I'd need to have the same variance in voltage with significantly smaller voltage | 21:38 |
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| nmz787_i | justanotheruser: ah, hmm, that seems to be the opposite of what I was thinking :) | 22:03 |
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| --- Log closed Wed Jun 15 00:00:30 2016 | ||
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