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| ebowden_ | kanzure, what happened to paperbot? | 01:28 |
|---|---|---|
| ebowden_ | Was it left useless by the takedown of libgen? | 01:28 |
| archels | it's redundant now, just use sci-hub | 01:38 |
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| ebowden_ | I seem to get directed to something of the same name that has a paywall. | 02:47 |
| ebowden_ | http://sci-hub.cc | 02:48 |
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| kanzure | ebowden_: there are many different scihub urls | 06:35 |
| ebowden_ | Good idea. | 06:36 |
| ebowden_ | God knows how many powerful people want them to shut down. | 06:36 |
| chris_99 | isn't there a tor hidden service | 06:39 |
| chris_99 | too | 06:39 |
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| JayDugger | Wow, laser projectors have gotten a whole lot cheaper since consumer electronics companies took over from aerospace companies. As in two orders of magnitude cheaper, although you don't have the near-IR in home units. So they won't work with NVGs. | 07:08 |
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| JayDugger | "Ridiculous that this isn't available to everybody." Yeah, perhaps you should cut back for a little while. | 07:10 |
| chris_99 | how much are they now, also how do they tend to work, similar to a DLP projector? | 07:11 |
| chris_99 | with a MEMS chip? | 07:11 |
| JayDugger | Down from over USD1000000 to about USD10000 or less. Overview here: http://www.cnet.com/news/why-lasers-are-the-future-of-projectors/ | 07:12 |
| chris_99 | aren't there really cheapo ones, i thought you can get a pocket one for <1k | 07:13 |
| JayDugger | Looks like it, from the CNET article. I'd have to look to see whether these match the ones from Raytheon, Evans & Sutherland, or Rheinmetall. | 07:13 |
| chris_99 | am i right in thinking with a laser projector, you could potentially make a decent HUD | 07:14 |
| JayDugger | Ah, not these as is, no. Think cinema or very large television in a fixed installation. | 07:16 |
| chris_99 | why not out of interest, i mean the small pocket ones | 07:17 |
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| chris_99 | ooh http://www.sony.com/electronics/portable-projectors/mp-cl1 | 07:20 |
| chris_99 | that's rather tempting | 07:20 |
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| CaptHindsight | Casio started selling some decently powered ones a few years ago https://www.youtube.com/watch?v=gF4HG6v29UY | 07:39 |
| CaptHindsight | ~$600 used on fleabay | 07:41 |
| chris_99 | ooh | 07:41 |
| chris_99 | i will have a looky | 07:41 |
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| chris_99 | how long would a laser diode last for do you know | 07:41 |
| CaptHindsight | http://www.ebay.com/itm/like/331815194881 | 07:42 |
| chris_99 | cool | 07:42 |
| CaptHindsight | 10k's of hours if kept cool enough | 07:42 |
| chris_99 | appaerntly that isn't purely a laser | 07:43 |
| chris_99 | http://www.casio.com/products/archive/Projectors/Slim_Projectors/XJ-A140 | 07:43 |
| CaptHindsight | ah the Google changed the search on me | 07:44 |
| CaptHindsight | how nice of them | 07:44 |
| CaptHindsight | you type in one part number and they return results for another | 07:44 |
| chris_99 | heh | 07:44 |
| chris_99 | whats the part no. of the one you were looking for | 07:44 |
| CaptHindsight | it's in the video | 07:45 |
| CaptHindsight | Casio A140 projector | 07:45 |
| chris_99 | i think that is the same as the one i linked to, but it also somehow uses an led somehow too? | 07:46 |
| CaptHindsight | I think they stopped selling the high powered laser version after all the hacking | 07:46 |
| chris_99 | ah | 07:46 |
| CaptHindsight | so look for used | 07:46 |
| CaptHindsight | some people ruin everything | 07:46 |
| chris_99 | http://www.ebay.co.uk/itm/REDUCED-Casio-XJ-A140V-DLP-LED-Projector-HDMI-VGA-2500-Lumens-/371692918939?hash=item568a9fd89b:g:2OEAAOSwARZXlfZU | 07:47 |
| chris_99 | could that be it | 07:47 |
| CaptHindsight | https://www.youtube.com/watch?v=ryLEMobdicw | 07:47 |
| chris_99 | that's very tempting heh, for ~£100 | 07:47 |
| chris_99 | what's happening in that video? i'm confused how the beams converge | 07:48 |
| chris_99 | then disperse | 07:48 |
| CaptHindsight | he steered them all to ~1ft in front of the lens | 07:53 |
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| chris_99 | ah | 07:55 |
| CaptHindsight | looks like the newer Casio SLIM XJ-A142 has the lasers | 07:56 |
| CaptHindsight | http://www.ebay.com/itm/BRAND-New-Casio-SLIM-XJ-A142-XGA-Laser-LED-Projector-2500-Lumens-Factory-Sealed-/152009792183 | 07:57 |
| CaptHindsight | maybe all the slim versions are laser since there isn't room for a lamp | 07:59 |
| chris_99 | mm, apparently the 140 one is 1024x768, but still seems a pretty good deal used | 08:00 |
| CaptHindsight | how will you use it? | 08:01 |
| CaptHindsight | burn stuff real good? | 08:01 |
| chris_99 | heh, just for movies | 08:01 |
| CaptHindsight | Casio XJ-M141 also appears to be lamp free | 08:03 |
| CaptHindsight | the casio site does a good job of listing the lamp free models | 08:05 |
| CaptHindsight | http://www.casio.com/products/Projectors/ | 08:06 |
| chris_99 | cool, i'll have a look | 08:06 |
| kanzure | greetings from california | 08:11 |
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| CaptHindsight | I recall reading about how the output rating of the projects has stayed the same but they use much lower powered laser leds now | 08:15 |
| CaptHindsight | so you still might want to find an older model that you can drive hard but add some extra cooling | 08:15 |
| chris_99 | ah interesting | 08:16 |
| kanzure | CaptHindsight: tell me things that are up | 08:16 |
| CaptHindsight | kanzure: just heading out to mix chems, be back much later | 08:17 |
| CaptHindsight | http://www.laserpointersafety.com/news/news/other-news_files/tag-casio.php#on | 08:20 |
| CaptHindsight | https://www.youtube.com/watch?v=IzUoe-9bKa0 | 08:20 |
| chris_99 | haha | 08:20 |
| chris_99 | that looks safe | 08:20 |
| CaptHindsight | Homebuilt 200W LASER BAZOOKA!!!!! | 08:20 |
| chris_99 | hehe | 08:21 |
| CaptHindsight | http://www.laserpointersafety.com/news/news/other-news_files/41601c4111261e7f9c6dcc086a06f9c8-186.php#on | 08:21 |
| CaptHindsight | Germany: "Iron Man" laser fires Class 4 beam from a hobbyist's palm | 08:21 |
| chris_99 | heh | 08:22 |
| CaptHindsight | at least no Yodas | 08:22 |
| kanzure | CaptHindsight: show me interesting photoresins | 08:26 |
| CaptHindsight | http://www.pnas.org/content/101/15/5488.full.pdf Photocleavable fluorescent nucleotides for DNA | 08:37 |
| CaptHindsight | http://www.genelink.com/newsite/products/mod_detail.asp?modid=133 | 08:37 |
| kanzure | yep | 08:38 |
| CaptHindsight | they have a bunch of PC stuff | 08:38 |
| kanzure | i think you should be looking at amino acids that are photoresponsive | 08:38 |
| CaptHindsight | or make your own | 08:38 |
| kanzure | anything about amino acid chemistry and photo-anything | 08:38 |
| CaptHindsight | bbl | 08:38 |
| kanzure | photoresponsive nucleotides are useful too and it's definitely going into that "electronically-directed polymerase" paper | 08:38 |
| kanzure | but amino acids that inflate to 10x their size upon illumination (or something) would be particularly useful | 08:38 |
| CaptHindsight | a team of useful people would also be useful | 08:39 |
| CaptHindsight | but where did they all go? | 08:39 |
| kanzure | gotta pay 'em | 08:40 |
| CaptHindsight | I'd have to dig them up | 08:41 |
| kanzure | graverobbing this early in the morning? unlikely | 08:41 |
| kanzure | re: an in vitro selection method for electronically-directed polymerase; an interesting first step would be CMOS array with polymerases acting as resistors (or other components, w/e) strung up between two electrodes, maybe a million or more per chip, and then merely testing the ability for different amino acids to allow for optical illumination to change electrical characteristics of the circuit as discriminated by sending signals down ... | 08:59 |
| kanzure | ... the device to each array element | 08:59 |
| kanzure | and this would be without nucleotide incorporation, without DNA synthesis activity, etc. | 08:59 |
| kanzure | really it needs to be a chip inside of an emulsion drop that could be remotely powered, so that you could have 10^12 emulsion drops and each one with different genotype-phenotype linkage and different mutant polymerase connected to the two antibody-conjugated electrodes. and then some sort of remote interrogation of this device, with optics and power transmission. then you pump a few thousand billion of these past a fluorescent emulsion ... | 09:01 |
| kanzure | ... drop sorting apparatus, which would be responsible for determining whether an emulsion compartment should be selected or not. | 09:01 |
| kanzure | the reason that compartments are necessary here is to separate genotype-phenotype from all the other samples, if there's a way to do that on a giant CMOS array without individual emulsion drops then that's fine and we should pursue that, although i don't know how to do it | 09:02 |
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| JayDugger | Those projectors I mentioned advertise lifetimes of about 20000 hours. | 09:20 |
| chris_99 | yeah so do the casio ones | 09:21 |
| chris_99 | i'm quite tempted by the cheapo 1024*768 one | 09:22 |
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| CaptHindsight | don't they teach you guys anything in EE school? | 09:43 |
| CaptHindsight | keep the led's cooler, then they will last longer | 09:43 |
| CaptHindsight | you don't really expect a consumer oem to make something that lasts for years | 09:44 |
| CaptHindsight | look up some articles on MTBF vs operating temperature | 09:44 |
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| chris_99 | could they not use some form of peltier | 09:52 |
| chris_99 | cooling | 09:52 |
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| streety | or just replace in 2 years | 11:11 |
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| kanzure | you could probably use surface-immobilized polymerase on a CMOS chip, then use surface patterning to make it so that emulsion droplets could get stuck to each region, perhaps using either liquid filling a container, or through surface patterning like the hydrophobic grating stuff. use inkjet printing to deposit the different materials into each emulsion droplet. have the underlying circuits capable of physical addressing. link ... | 12:48 |
| kanzure | ... genotype-phenotype inside each compartment, for whatever mutant polymerase is used inside that compartment. bottom of the compartment is the interface with the chip. individual genes can be recovered from this setup by using micropipetting to the specified (x,y) coordinates. | 12:48 |
| kanzure | if they are physically rescessed wells then there is probably no reason to bother with emulsion containment, as long as you do not have liquid spillover from one well to another | 12:50 |
| kanzure | rescinded wells? rescessed? whatever. | 12:50 |
| kanzure | interestingly, once you have that basic chip working, you can reuse it for most of those polymerase evolution projects because the basic way of conjugating a polymrase to a circuit doesn't require any changes - it's the polymerase that is being changed. | 12:51 |
| archels | "Introduction to Brains as Emergent Turing Machines" | 13:02 |
| archels | this ought'a be good | 13:02 |
| archels | the near- and long-term applications slides in the back are empty | 13:05 |
| archels | (there are 83 slides in total) | 13:06 |
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| kanzure | the inkjetting requirement above is merely that mutant polymerases (and their genes) get distributed over the surface of a chip, and that the gene and polymerase both get into the same droplet, but it doesn't matter where on the surface they go. the other requirement is that each location with the polymerase hooked up to the circuit needs to be physically addressable by a pipetting machine, for selecting that element for PCR. it's okay ... | 13:53 |
| kanzure | ... to have polymerases that don't bind to a junction, and it's okay to have emulsion drops that don't get used but it's important that only one emulsion drop lands on a region on the chip (and not two or more emulsion compartments) because that causes genetic mixing. | 13:53 |
| kanzure | and i think an inkjet printhead could be made to deposit emulsion drops like that on to a surface. | 13:54 |
| kanzure | perhaps even with the energy from impact, the drops still wouldn't merge together? or perhaps there's an oil-in-water emulsion that will prevent compartments from merging and mixing in that scenario? | 13:55 |
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| CaptHindsight | make drops from an inkjet stop, move and stop on a tray | 14:39 |
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| kanzure | eriqoejriqejiadfiadfqioieqr | 22:20 |
| KilometersDavis | I see | 22:26 |
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| kanzure | yea it's a problem | 22:33 |
| nmz787 | sup | 22:37 |
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| kanzure | hanging out in california | 22:50 |
| kanzure | nmz787: https://twitter.com/kanzure/status/759560626498777088 | 22:51 |
| nmz787 | hmm, I think I drove 130 miles today | 22:51 |
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| maaku | That's 150 words per mile. Keep it up. | 23:24 |
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| --- Log closed Sun Jul 31 00:00:12 2016 | ||
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