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| archels_ | .title http://www.cell.com/cell/abstract/S0092-8674(13)00770-8 | 01:51 |
|---|---|---|
| yoleaux | archels_: Sorry, that doesn't appear to be an HTML page. | 01:51 |
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| kanzure | adlai: i must have lobotomied that particular cortex, i don't seem to have that | 03:54 |
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| JayDugger1 | I have a much less tasteful joke about Baby Boomers. | 06:13 |
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| wrldpc1 | I love less tasteful jokes about baby boomers. | 06:22 |
| wrldpc1 | Old Economy Steve is hilarious but grossly inadequate. | 06:22 |
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| nmz787_ | .wik expansion microscopy | 11:55 |
| yoleaux | "Expansion Microscopy (ExM) is a sample preparation tool for biological samples that allows investigators to identify small structures by expanding them using a polymer system." — https://en.wikipedia.org/wiki/Expansion_microscopy | 11:55 |
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| nmz787_ | uses baby diaper chemical and hydrogel, embed a sample in the gel, polymerize, then digest sample and expand gel by adding water | 11:56 |
| nmz787_ | heh, using DNA oligos as linkers | 11:58 |
| chris_99 | nmz787_, you played with fixing the SEM you got, of late, out of interest? | 11:59 |
| nmz787_ | chozinksi et al. Nature Methods 2016 | 11:59 |
| nmz787_ | chris_99: routed some new wires from my power panel in my house last month, need to break the wire ends out in the lab room and install the plugs | 12:00 |
| nmz787_ | chris_99: but no, need power first | 12:00 |
| chris_99 | ah, does a sem require a fair amount of power out of interest, or do they just run from a normal plug | 12:01 |
| chris_99 | i'm assuming the latter | 12:01 |
| chris_99 | also in the US, are you allowed to wire up your whole house out of interest, we have a stupid law that prevents that now | 12:05 |
| kanzure | greets | 12:15 |
| nmz787_ | it takes 10-15 amps at 100V AC | 12:17 |
| nmz787_ | but my office/lab was already on one circuit with a bathroom and utilityroom lights | 12:17 |
| nmz787_ | chris_99: laws like this mostly depend on the state, but standards are set by state and fed | 12:17 |
| nmz787_ | so in Oregon homeowners can do any works they want, as long as it conforms with code and they get permits (which come with inspections) | 12:17 |
| nmz787_ | but not all work needs permits | 12:17 |
| nmz787_ | hmm, reverse shrinky-dink for chemical synthesis? | 12:17 |
| chris_99 | aha gotcha, is 15 amp via a normal plug then i guess? | 12:18 |
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| nmz787_ | well we have 110-120VAC so I have a variac to get it to 100V | 12:27 |
| chris_99 | ah interesting | 12:28 |
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| nmz787_ | .g biowave pro | 12:52 |
| yoleaux | http://ipv6.google.com/sorry/index?continue=http://www.google.com/search%3F%26q%3Dbiowave%2520pro%26btnI%3D&q=EhAqAQT4AgFyMAAAAAAACGrnGOSq7sAFIhkA8aeDS1qaziOHmVtDHOlsKibXkdLU1dnjMgFj | 12:52 |
| nmz787_ | I wonder how well it makes popcorn | 12:52 |
| nmz787_ | .wik antigen retrieval | 12:59 |
| yoleaux | "Tissues that have been preserved with formaldehyde, a highly reactive compound, contain a variety of chemical modifications that can reduce the detectability of proteins in biomedical procedures such as immunohistochemistry. Antigen retrieval is an approach to reducing or eliminating these chemical modifications." — https://en.wikipedia.org/wiki/Antigen_retrieval | 12:59 |
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| kanzure | https://gitter.im/Keras-io/Lobby | 13:41 |
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| kanzure | softmax activation stuff https://www.tensorflow.org/versions/r0.11/tutorials/mnist/beginners/index.html | 14:28 |
| kanzure | http://neuralnetworksanddeeplearning.com/chap3.html#softmax | 14:28 |
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| kanzure | blooop | 15:59 |
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| juri_ | meep. | 16:28 |
| kanzure | if you're a roadrunner then the irc channel is just a giant painting | 16:39 |
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| nmz787_ | http://www.nature.com.sci-hub.cc/nsmb/journal/v22/n5/pdf/nsmb.3014.pdf# | 23:06 |
| nmz787_ | PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome | 23:06 |
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| --- Log closed Fri Nov 04 00:00:40 2016 | ||
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