2008-04-14.log:22:52 < fenn> yes, telomeres etc
2008-08-22.log:14:30 < kanzure> Leukocyte telomere length is associated with cognitive performance in healthy women 
2009-10-03.log:17:00 < katsmeow-afk> i assume you are wrapping up  telomeres, or keeping them from falling off? how can that cause uncontroleld reproducton, since native stem cells don't?
2009-10-05.log:14:45 < kanzure_> cool telomeres made the 2009 nobel prizes
2010-03-24.log:15:55 < kanzure> 17:03 < bdesk> kanzure will save us from the relentless shortening of our telomeres with his singularity-accelerating self replicating universal 
2010-03-24.log:15:55 < kanzure> 17:03 < bdesk> kanzure will save us from the relentless shortening of our telomeres with his singularity-accelerating self replicating universal fabricators
2010-06-28.log:18:35 < kanzure> blah a telomere thing?
2010-07-03.log:19:16 < bdesk> every cell selectively turns certain genes on or off.  this is what transcriptional regulation is.  i guess they are talking about cell 'potency'.  also i wonder if the transdifferentiation also re-elongates the telomeres.
2010-09-20.log:11:12 < kanzure> probably another RGS14 or telomere newsblitz
2010-10-05.log:09:51 < dbolser> did you know average telomere length is correlated to omega 3 intake and 'chronic stress'?
2010-10-05.log:09:51 < dbolser> kanzure: I was thinking that a quick and dirty telomere length self determination kit would be quite a hit
2010-10-05.log:09:52 < dbolser> you can track your own telomer length in real time, and watch how your new exercise program promotes good physiological health 
2010-10-05.log:09:52 < dbolser> also, short telomeres are predictive of various common diseases - typically 'age related disease'
2010-10-05.log:09:55 < dbolser> a 3 month intensive meditation course lead to improved telomere length / welbeing correlation...
2010-10-05.log:09:58 < dbolser> if you can read off your telomere length (average), at least there is omething measurable
2010-10-05.log:09:58 < fenn> how do they measure telomere length?
2010-12-11.log:10:58 < kanzure> evolv: telomerase is just one factor out of many in aging :P
2010-12-11.log:10:59 < kanzure> evolv: yeah but radiation damage + telomerase is not the full story.
2010-12-11.log:11:08 < kanzure> bdesk: most people who believe in magic bullet cures to aging use the genetic regulatory network cascading collapse model (instead of death hormone) or oxidation damage or telomerase damage models
2010-12-11.log:11:09 < delinquentme> do the "super oxides" Vladomir Putin talks about shorten telomeres?
2010-12-11.log:11:22 < kanzure> "The need to return to the clinic exists because a WILT recipient has no more telomerase anywhere, including - especially - the stem cell populations vital to health. There are genetic diseases that tell us what happens when your stem cells stop being able to lengthen their telomeres; it's a slow and unpleasant way to go, taking about a decade to show up in earnest, and running downhill from there as regeneration and repair starts to shut down."
2010-12-11.log:11:23 < kanzure> "So the WILT recipient of 2030 would have to receive new stem cell populations, or more likely some form of gene therapy that restored telomere length in the existing populations, every decade or so."
2010-12-11.log:11:25 < delinquentme> dbolser, ummm when cells divide they snip a bit of the telomere off the end ... 
2010-12-11.log:11:26 < delinquentme> but i thought INSTEAD that the CELLS no longer replicate in a clean manner after the telomeres are gone?
2010-12-11.log:11:27 < delinquentme> bdesk, im confused though .. w the harvard telomere research .. they were talking about extending the telomeres resulting in cancer
2010-12-11.log:11:28 < delinquentme> telomeres ... WHICH WOULD ... result in cancer ..e xcuse me
2010-12-11.log:11:29 < kanzure> they genetically engineered the mice to have retarded telomerase
2010-12-11.log:11:29 < kanzure> so guess what happens when you fix the telomerases!
2010-12-11.log:11:31 < delinquentme> cancerous cells reproduce @ furious rate ( duh ) .. the reproduction causes the telomeres to wear away hella quick ... and when a cell rusn out of telomeres it self-destructs
2010-12-12.log:06:59 < dbolser> 14:25 < delinquentme> dbolser, ummm when cells divide they snip a bit of the telomere off the end ... 
2010-12-13.log:08:33 < kanzure> phryk: if you fuck up telomeres in mice and then unfuck-up the telomeres then of couse they will improe in condition..
2010-12-13.log:08:45 < kanzure> "On quick read it looks like they accelerated aging in the mice by knocking out telomerase and then reversed the accelerated aging by injecting/inducing telomerase.  The question is: Are these mice any better off than unaltered mice (true controls)?"
2010-12-13.log:08:46 < kanzure> "We published a paper last year showing that CR in mice (CR extends lifespan in this strain) substantially REDUCES telomerase expression (about 3-fold in liver, a proliferative tissue, http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0005242). This is further evidence that telomerase activity and telomere length are responsive to other aspects of biology and in most organisms (including humans) telomere length is probably a better 
2010-12-13.log:08:46 < kanzure> "However--and this is a major caveat--in theory, short telomeres can limit lifespan in some individuals because natural selection is weak in older age and telomere length is one of those parameters for which there is little or no selective pressure to get just right."
2010-12-13.log:08:46 < kanzure> "Therefore, the strong prediction is that telomere activation (either in healthy mice or humans) will increase mean and median lifespan by an amount that is difficult to predict, but it will not increase maximum lifespan--possibly even reducing it by increasing the risk of metastatic cancer in very old people who otherwise would have avoided it."
2010-12-13.log:08:46 < kanzure> "Therefore, I have the opposite view from DePinho's: I don't think young people should take telomerase activators unless they know they have an inordinately large fraction of short telomeres. It is relatively old people who probably have the greatest benefit to risk ratio, but these are just wild guesses from both of us."
2010-12-13.log:08:46 < kanzure> "The only way to know for sure is to collect telomere length and mortality data on animals (including people) with and without administration of telomerase activators."
2011-01-01.log:11:03 < archels> Worth buying? The suggestion is apparently that we can influence the length of our telomeres via diet/exercise/etc.
2011-01-11.log:19:04 < kanzure> life extension founation just announced that they are spending $2M in funding on telomere lengthening research
2011-01-13.log:14:00 < kanzure> telomerase patent was granted today
2011-06-06.log:22:20 < fenn> weird longevity factoid: kids raised in orphanages have shorter telomere length than kids raised in foster care
2012-01-23.log:20:23 < yashgaroth> aside from telomerase? I'll take a look
2012-01-23.log:20:30 < yashgaroth> but afaik, generating a "new" DNA strand without a template is limited only to telomerase, and that technically has a template
2012-02-17.log:21:11 < kanzure> http://mariakonovalenko.wordpress.com/2012/01/14/telomeres-and-aging-chart/
2012-03-17.log:21:28 < Thorbinator_> did you activate the transcription for telomerase?
2012-04-06.log:13:01 < kanzure> "I have strong ethics and no morals and I am only out to serve myself and the enhancement of my own telomeres." jonathan is keeping it real on diybio
2012-05-01.log:13:55 < yashgaroth> oh god some dumbass on biohack.me wants to genemod himself with telomerase in an HIV vector
2012-05-01.log:13:56 <@kanzure> also.. did he have a reason for telomerase?
2012-05-01.log:13:57 < yashgaroth> 3 telomerase by itself won't do anything but make him more susceptible to leukemia
2012-05-01.log:13:59 < nmz787> overexpression of telomerase would lead to leukemia why?
2012-05-01.log:13:59 <@kanzure> telomerase by itself wont cause cells to live longer
2012-05-01.log:14:09 < yashgaroth> normally the body uses telomeres to regulate cell death, since a cell with short telomeres is likely to have a lot of mutations
2012-05-01.log:14:10 < yashgaroth> I won't get into the obsession that amateur biologists seem to have with telomerase in general, but by itself it won't do much to help lifespan
2012-05-01.log:23:43 < yashgaroth> I threw up a response to that telomerase fellow on biohack if you want something more cogent than my previous rambling
2012-05-03.log:20:25 < kanzure> "then you need a technique to synthesis telomerase from the polymerase"
2012-05-03.log:20:28 < yashgaroth> someone saying 'telomerase' in almost any context is a strong warning sign, though in this case it was quite obvious before he says that
2012-05-11.log:15:10 < yashgaroth> news update, some guy on biohack wants to do that telomerase virus thing on his 80 year old grandma
2012-05-11.log:15:12 <@kanzure> why is he so focused on telomerase, again?
2012-05-14.log:22:16 <@kanzure> "virally delivered telomerase extends lifespan of 1-year old mice by 24%"
2012-05-14.log:22:31 < yashgaroth> I don't think they'd be able to measure an increased risk of cancer, since mice don't get it as often as humans and telomerase might not even be a limiting factor for it
2012-05-15.log:14:07 <@kanzure> random telomerase-related email i've received:
2012-06-23.log:18:53 < yashgaroth> more accurate title/quote: "scientists have discovered that in sperm, telomeres lengthen with age"
2012-08-02.log:10:29 < kanzure> "But TA Sciences has taken the buzz further: it sells a pill called TA-65, which it says can lengthen short telomeres. The pill brings in an annual revenue of $6 million in the United States alone."
2012-08-02.log:10:29 < kanzure> "he active ingredient of TA-65 was isolated from the herb Astragalus membranaceus and patented by Geron, a biopharmaceutical firm in Menlo Park, CA. Research sponsored by TA Sciences and other companies has shown that the compound can lengthen telomeres in mice 2 and humans 3, but Greider and others are skeptical of the assay used."
2012-10-04.log:21:12 < yashgaroth> it's like a minor, brain version of the telomerase idiocy
2012-10-04.log:21:24 < yashgaroth> you know, the "let's add telomerase to every cell somehow and I'll never die"
2012-10-04.log:21:27 < brownies> extend ALL the telomeres
2012-10-04.log:21:29 < yashgaroth> upregulating telomerase by itself will make you die of cancer faster, so all the studies are either with special cancer-resistant mice, or 'we engineered mice to not have telomerase and they were all shitty, then we gave them back telomerase and they were okay, what a fucking surprise'
2012-10-04.log:22:05 < BioGuy> Though I'm curious why do you think re-activating telomerase wouldn't lead to greater longevity?
2012-10-04.log:22:06 < yashgaroth> because it's used by the body to limit the prevalence of cancer; stem cells have telomerase, and I have my private theory that most cancers derive from stem cells rather than somatic cells
2012-10-04.log:22:07 < yashgaroth> without telomerase a cancer will crap out and die pretty quick
2012-10-04.log:22:08 < yashgaroth> and every method to induce telomerase post-natally, usually some sort of viral vector with the telomerase gene, will only affect superficial cells that die off naturally with no negative consequence
2012-10-04.log:22:09 < yashgaroth> but no one wants to discuss germline gengineering, and so you get skin creams infused with recombinant telomerase and sell it to morons
2012-10-04.log:22:12 < BioGuy> Oh... so your not suggesting that telomerase wouldn't be a major factor in increasesd longevity, but that the problem is just more complicated than just re-activate telomerase in every single cell?
2012-10-04.log:22:21 < BioGuy> It would be interesting to see a mouse model study where one set of mice somehow had telomerase reactivated in as many cell types as possible and another set of 'normal' mice, feed them both food or water with some carcinogenicity and then look at which sets have higher prevelance of cancer.
2012-10-04.log:22:22 < yashgaroth> I think it's been done and they get more cancer, but I haven't been keeping up on the literature since putting 'telomerase' in the article automatically discourages me from reading
2012-11-22.log:06:39 < archels> ltl = leukocyte telomere length
2012-11-28.log:18:55 <@kanzure> yashgaroth: what was the telomerase study where they disabled telomerase, then added it back in and pretended it was a miracle?
2012-12-15.log:18:57 <@fenn> there are several theories of aging which may all be correct: senescence due to telomere depletion, hormonal miscommunication, damage due to free radicals, damage due to glycation
2012-12-25.log:16:37 < yashgaroth> (please don't say telomerase)
2012-12-25.log:17:50 < yashgaroth> hey I'm just glad to have someone here interested in biology that isn't talking constantly and ignorantly about telomerase
2012-12-29.log:11:27 <@kanzure> barriers: telomerase isn't going to do that much good
2012-12-29.log:11:35 <@kanzure> no, but i guess sens is a reasonable place to start if you only know about telomeres.
2013-01-14.log:22:06 < yashgaroth> they also require growth factors for survival, it's just that if you have said factors they won't eventually lose their telomeres and die
2013-03-07.log:10:08 <@kanzure> "The inaugural awards, however, went to a bumper crop: 11 pioneers who will help to select winners in subsequent years.  This year’s winners include those working in genomics (David Botstein and Eric Lander), cancer (Lew Cantley, Hans Clevers, Napoleone Ferrara, Charles Sawyers, Bert Vogelstein and Robert Weinberg), telomeres (Titia de Lange), stem cells (Shinya Yamanaka) and neurobiology (Cori Bargmann). "
2013-03-08.log:22:20 <@kanzure> telomere testing campaign thing http://www.indiegogo.com/projects/telome-telomere-testing-take-control-of-your-long-term-health
2013-04-06.log:11:40 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/Identification%20of%20seven%20loci%20affecting%20mean%20telomere%20length%20and%20their%20association%20with%20disease.pdf
2013-05-23.log:21:04 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Intramolecular%20folding%20in%20three%20tandem%20guanine%20repeats%20of%20human%20telomeric%20DNA.pdf
2014-04-25.log:00:39 < fenn> i thought the telomere hypothesis was disproven
2014-05-04.log:13:34 < fenn> including telomere theory and oxidative damage
2014-12-25.log:11:11 < kanzure> did you ever take a look at cathal's proposal to use telomerases? https://groups.google.com/d/msg/enzymaticsynthesis/6GZT8zFNOfo/npfYUhf1HJ8J
2014-12-25.log:11:14 < yashgaroth> perhaps I need a closer look at the telomerase one, but if you have 3+ of the same base in a row, wouldn't you get runaway addition? will check out the igem one now
2015-02-03.log:01:11 < archels> Hayflick limit being ascribed solely to telomere length
2015-10-10.log:07:36 < kanzure> ah this is a better summary, as usual https://www.fightaging.org/archives/2015/10/bioviva-moving-ahead-with-human-gene-therapy-for-telomerase-activation.php
2015-10-10.log:07:36 < kanzure> ok just telomerase activation stuff, sorta strange choice for them to go with for gene therapy but ok
2015-10-18.log:16:12 < delinquentme> telomerase being one of these molecules
2015-10-18.log:16:47 < atomical> if the telomerase gene therapy worked would she start to look younger?
2015-10-18.log:16:48 < yashgaroth> I have professional disagreements with her science team about the usefulness of telomerase, but probably no
2015-10-18.log:16:49 < kanzure> you'll have to ask all the mice that telomerase extension was tested on- did their mates look younger? y/n
2015-10-18.log:16:55 < atomical> how is Liz Parrish going to convince people that telomerase therapy actually did something?
2015-11-16.log:12:41 < haskel> kanzure, here's a target: telomerase gene therapy, we can precisely print he dna to create telomerase, so we're done right? since delivery is so easy and not an issue and easier than the former?
2015-11-16.log:12:41 < kanzure> haskel: because i don't see much value in "full proof of concept". i'm completely convinced that gene therapy works for certain definitions of work. telomerase gene therapy is completely unconvincing, though.
2015-11-16.log:12:42 < kanzure> yea if you don't fuck up your telomeres, you're just playing around
2015-11-16.log:12:50 < kanzure> haskel: you can't just wave your hand and get magical phenotype changes. that's not how biology works. nobody knows the exact mapping from genotype to phenotype for all possible changes. telomerase changes aren't going to produce easily observable effects for a long, long time. it's a bad "proof of concept".
2015-11-16.log:12:57 < kanzure> research prospects are v. important to speed up, but there's a lot of low-hanging engineering fruit, telomerase doesn't seem to be one of them- mouse results are OK, but i don't care. show me a better demo, like restoring color/infrared vision.
2015-11-16.log:13:01 < haskel> kanzure, " telomerase doesn't seem to be one of them- mouse results are OK" can you be a bit more specific?
2015-11-16.log:13:02 < haskel> kanzure, I used the specific example of a mouse to give some evidence, can you give a specific example why telomerase gene therapy isn't worth exploring atm?.
2015-11-16.log:13:02 < kanzure> the mouse results for telomerase modification seem to be OK results, but i don't have 200 years to wait to see whether that works for humans
2015-11-16.log:13:05 < kanzure> haskel: not really; you can perform a lot of rejuvenation work on super old people. but telomerase ain't going to be enough. it's not enough to show results in aging populations, in my opinion. unless you do large-scale statistical studies i guess... but i don't care about populations, i care about individuals.
2015-11-16.log:13:12 < kanzure> haskel: i think you would be wise to drop the accusational tone and look more closely at existing technology. also, you should not assume that telomerase is a magic silver bullet. results come from broad technology fundamentals building up over time and technical tools becoming better, not from riding a magic bullet into the singularity.
2015-11-16.log:13:14 < haskel> kanzure, telomerase is one direction of many agreed, I mentioned that to give a specific example when you said there are no good gene therapy targets atm
2015-11-16.log:13:18 < andares> Yeah, and I just saw one thread about hijacking telomeraaw
2015-11-16.log:13:40 < haskel> kanzure, and the channel's name is hplus**roadmap** which is essentially what I was trying to get at lol, thanks for answering my questions, fenn thank you as well, I think it is important to speed up the computational/experimental loop with cheap de novo synthesis, but I think we must also explore more immediate gains such as telomerase gene therapy etc. which involves getting the delivery part right, which I think is no where near done
2015-11-16.log:13:41 < kanzure> instead of telomerase i suggest looking at http://diyhpl.us/wiki/genetic-modifications/ and http://diyhpl.us/wiki/dna/projects/#igem-2013 etc
2015-11-16.log:13:42 < fenn> i am not convinced that telomerase is important, but gene therapy needs some work for sure
2015-11-16.log:13:43 < haskel> fenn, agreed, I definitely think telomerase is worth exploring, it's had an effect on mice, and it has a clear physical link to cell aging, and there is a clear method to restore telomeres
2015-11-16.log:13:48 < haskel> fenn, but choline is involved in a lot of things, whereas literally the only purpose of telomerase is to restore telomeres which shorten each time the cell divides, that can't be good for your genes
2015-11-16.log:13:50 < haskel> fenn, it's been a while for me, is there any other established role of telomerase ?
2015-11-16.log:13:50 < fenn> i have no idea what telomeres are for
2015-11-16.log:13:51 < FourFire> AFAIK the telomere is a correlation assumption: "oh the dna gets fucked up when these things run out, then that must be why blah blah"
2015-11-16.log:13:52 < haskel> FourFire, so a way to prevent that is to lengthen the telomeres, which I understand telomerase will do
2015-11-16.log:13:55 < haskel> FourFire, is there any other established role of telomerase? (there may be some other role telomerase plays, but I think atm that is the only role we have established)
2015-11-16.log:13:55 < fenn> 'telomeres may have a role in the prevention of cancer. This is because the telomeres act as a sort of time-delay "fuse"'
2015-11-16.log:13:56 < haskel> fenn, but is that not through the same mechanism of action, keep telomeres long?
2015-11-16.log:13:56 < fenn> aubrey's solution is to kill telomerase because tumors use it to bypass the hayflick limit
2015-11-18.log:05:58 < kanzure> blocking PARP1 and upregulating/activating ATM kinase results in telomere lengthening ("ATM Kinase Is Required for Telomere Elongation in Mouse and Human Cells") http://www.cell.com/cms/attachment/2040375994/2053862741/mmc1.pdf http://www.cell.com/cell-reports/abstract/S2211-1247(15)01205-X?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS221112471501205X%3Fshowall%3Dtrue
2015-12-04.log:16:55 < atomical_> "The myostatin inhibitor has been tested already. We should start seeing results around 4-5 months and peak around 8-12. Although we could extrapolate from the myostatin inhibitor, we do not know how long it will take the telomerase induction to begin working because no one has done it before."
2015-12-04.log:17:11 < kanzure> pasky_: summary is "someone claims to have used adenovirus/retrovirus-based gene therapy for telomerase extension lengthening and follistatin expression, something something south america, hplusroadmap wrote an angry letter to her suggesting that she chooses different visual reporter indicators for whether the gene therapy works"
2015-12-04.log:17:13 < kanzure> i mean, it's true that telomerase hsa been investigated in previous experiments regarding aging, but that's not saying much
2015-12-04.log:17:13 < kanzure> *telomerase has been
2015-12-04.log:17:13 < kanzure> *telomerase extension lengthening
2016-01-11.log:07:21 < kanzure> However, given the fact that the work was carried out on mTerc-/- mice, and that mice have quite different telomere lengths to humans, (and therefore possibly different mechanisms involved) I think it is very premature to suggest that equivalent effects would be possible in humans. I think it is drawing a very long bow indeed. I would be delighted if someone could extend this work to higher organisms or perhaps, by manipulation of the ...
2016-01-11.log:07:22 < kanzure> ... systemic environment, to humans. Until then, your oft-repeated mantra: "heterochronic transplantation of tissues and organs in mammals show rejuvenation of the old tissue/organ in the young body." should be amended to include "in a telomere-deficient mouse model".
2016-01-24.log:13:57 < AdrianG> so its just telomerase and follistatin-something.
2016-02-25.log:03:06 < archels> telomerase (involved in regulating ageing) at a clinic outside the US (Regalado 2015).
2016-04-23.log:13:36 < kanzure> "In March 2016, the same tests were taken again by SpectraCell revealed that her telomeres had lengthened from 6.71kb to 7.33kb"
2016-04-23.log:13:38 < yashgaroth> yeah she had some tests done, not that I trust telomere length assays; more interested in pre- and post-exposure levels of AAV-neutalizing antibodies, but we'll see
2016-04-24.log:15:21 < kanzure> "In a longitudinal study testing telomere length in a large human cohort, 44% of people had longer telomeres than when they were 10 years younger (and 10 years is a lot of aging!) http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004191 So even if her telomeres did get longer, how confident can we be that is at all related to the gene therapy?"
2016-07-31.log:16:57 < CaptHindsight> "looks like your telomeres are too short, would you like to order some longer ones from the Apple Store?"
2016-07-31.log:16:58 < CaptHindsight> worse yet "your subscription for longer telomeres has expired, please insert $100k for another 12 subscription"
2016-08-08.log:21:53 < nmz787_> a more reasonable response just after:  "18:45 < maaku> mgin: because humans suck and need to die"  would have been something pointing to telomeres and shitty bugs in 'human code' which literally in a formal verification kind of sense means we MUST die, or at least the inverse of we haven't been able to prove a way NOT to die
2016-12-10.log:11:59 < Fausta> she put telomerase mRNA into cells in a dish and got a transient (not perm=cancer) increase in telomere length
2016-12-10.log:12:01 < yashgaroth> well yeah you're not gonna induce cancer just by adding telomerase, but it's an important regulatory check on cancer cells
2016-12-10.log:12:01 < yashgaroth> telomeres govern the hayflick limit in cultured cells but it's a tiny fraction of the problem
2016-12-10.log:12:57 < yashgaroth> fausta you seem hung up on telomeres, when that sounds more like malnutrition/lack of sleep/mental health/etc
2016-12-10.log:12:59 < Fausta> how can emotional stress make your skin less elastic; I feel it must be a telomere thing
2016-12-10.log:13:01 < Fausta> yes, which made me wonder if my telomeres got longer but prob it was just cortisol
2017-01-17.log:07:04 < kanzure> "TZAP: A telomere-associated protein involved in telomere length control" http://science.sciencemag.org/content/early/2017/01/11/science.aah6752
2017-02-13.log:20:46 < kanzure> "Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolis...
2017-02-13.log:20:46 < kanzure> ...m at non-telomeric sites."
2017-02-14.log:06:55 < kanzure> "DCAF4, a novel gene associated with leucocyte telomere length" http://jmg.bmj.com/content/early/2015/01/23/jmedgenet-2014-102681.full
2017-02-14.log:06:57 < kanzure> "Mangino et al. (2015) found an association between an intronic G-to-A transition (rs2535913) in the DCAF4 gene and leukocyte telomere length (p = 6.4 x 10(-10)). The minor A allele was associated with shorter LTL (p = 7.8 x 10(-3)). In a second part of the study, the minor A allele was associated with lower expression of DCAF4 in lymphoblastoid cells from 778 twins of European descent, in who...
2017-02-14.log:06:57 < kanzure> ...le blood of 167 individuals, and in sun-exposed skin from 113 individuals; telomere length in these individuals was not reported. Noting that the DCAF4 gene is involved with proteins in the DNA repair pathway and is expressed in sun-exposed skin, Mangino et al. (2015) postulated that the role of DCAF4 in affecting telomere length may suggest a potential role for this gene in the development of...
2017-02-22.log:06:03 < yoleaux> Acute exercise activates p38 MAPK and increases the expression of telomere protective genes in cardiac muscle. - PubMed - NCBI
2017-04-04.log:19:23 < yashgaroth> or like telomerase and other template-independent enzymes, but they only list a protocol for tdt
2017-04-09.log:19:20 < yashgaroth> and he's a bit more optimistic about e.g. telomerase
2017-04-13.log:13:11 < jcorgan> read meat consumption correlated with telomere length
2017-04-21.log:19:06 < kanzure> ".. ZNF217 can attenuate apoptotic signals resulting from telomere dysfunction and may promote neoplastic transformation and later stages of malignancy"
2017-04-27.log:09:18 < eudoxia> oh, are you getting the telomere therapy?
2017-05-08.log:15:49 < kanzure> Ramunas, J., Yakubov, E., Brady, J.J., Corbel, S.Y., Holbrook, C., Brandt, M., Stein, J., Santiago, J.G., Cooke, J.P., and Blau, H.M. (2015). Transient delivery of modified mRNA encoding TERT rapidly extends telomeres in human cells. FASEB J fj.14-259531.
2017-05-15.log:13:15 < kanzure> "put in an extra copy of p53 - with all the same controls, e.g. transcription factor binding site on promotors, enhancers; that seems to work well for whales, they don't get cancer in spite of the enormous numbers of cell divisions they go through to get to their size (and longer telomeres?)"
2017-08-06.log:10:24 < yashgaroth> I doubt telomeres help much in alzheimer's, for example
2017-10-01.log:09:04 < kanzure> "Gene therapy with the TRF1 telomere gene rescues decreased TRF1 levels with aging and prolongs mouse health span" http://onlinelibrary.wiley.com/doi/10.1111/acel.12677/full
2017-10-01.log:09:05 < kanzure> "The shelterin complex protects telomeres by preventing them from being degraded and recognized as double-strand DNA breaks. TRF1 is an essential component of shelterin, with important roles in telomere protection and telomere replication. We previously showed that TRF1 deficiency in the context of different mouse tissues leads to loss of tissue homeostasis owing to impaired stem cell ...
2017-10-05.log:19:02 < kanzure> http://www.nanalyze.com/2017/04/what-is-telomere-what-telomeres-do/ "The thing is though, if you want to lengthen your telomeres, you can go use the TA-65 supplement which has been shown in a published human study to lengthen telomeres for over a period of 12 months. The study in question was conducted with 97 men and women in a controlled environment, not just with one person. Here’s that ...
2017-10-07.log:15:23 < yashgaroth> assuming it reaches the nucleus, yeah it'll eventually fade away as the daughter cell that carries it runs out of telomeres and dies
2017-10-10.log:16:37 < yoleaux> @mattloose @86Dominik @ClarksysCorner @nanopore Yes, two yeast chr were spanned by a single nanopore read from telomere to telomere (~220Kb). Could send the reads if interested! (@J_M_Aury, in reply to tw:917410699315503104)
2017-10-28.log:13:39 < kanzure> geeze i forgot about telomerase
2017-10-28.log:13:39 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f23e75c8 Bryan Bishop: forgot about telomerase >> http://diyhpl.us/diyhpluswiki/gene-editing/
2017-10-28.log:14:46 <@nmz787> kanzure: oh yeah, telomerase... hmm
2018-01-24.log:19:43 < fenn> embryonic stem cells express telomerase, so presumably they could be an immortal cell line...
2018-02-10.log:11:37 < kanzure> www.rechargebiomedical.com/liz-parrish-and-2yr-follow-up-from-telomerase-gene-therapy
2018-03-09.log:12:18 < nmz787> mix telomerase with a fresh placenta?
2018-03-09.log:12:22 < kanzure> how would telomerase mixing help?
2018-03-09.log:12:24 < nmz787> obviously adding telomerase will extend the telomeres and allow the placenta to reach past the hayflick limit, geez
2018-03-15.log:18:39 < yoleaux> "Microcell Mediated Chromosome Transfer (or MMCT) is a technique used in cell biology and genetics to transfer a chromosome from a defined donor cell line into a recipient cell line. MMCT has been in use since the 1970s and has contributed to a multitude of discoveries including tumor, metastasis and telomerase suppressor genes as well as  …" — https://en.wikipedia.org/wiki/Microcell-mediated_chromosome_transfer
2018-04-17.log:16:17 < yashgaroth> theoretically, they're selling AAV vector delivering genes for: p53 for the cancer stuff, telomerase for the 'aesthetics' and organs, and follistatin for muscle...not sure about alzheimer's and erectile dysfunction
2018-04-25.log:14:16 < Mathias_Mindblad> Yeah you keep the lines of telomeres from changing length, and you won't have any lingering "Zombie Cells" causing brittle bones and reduced skin elacticity."
2018-04-25.log:14:19 < Mathias_Mindblad> If you can eliminate free radicals in the body, and keep the telomere lines from changing, you just won't age anymore, and you may regenerate into a 21 year old body because the effects of aging would wash through with all the dead cells that are replaced by new ones continuously.
2018-04-27.log:17:42 < fenn> the more i learn about telomerase, the harder it is to see a way to hack it to produce arbitrary sequences
2018-04-27.log:17:44 < kanzure> what is the actual structure of telomerase?
2018-05-21.log:02:55 < fenn> the label "telomerase extends leading strand repeats" should go underneath the enzyme like the other labels
2018-08-27.log:01:33 < Akh194> anyone present taking any anti-aging supplements? if so whats good? seems there's a ton of stuff out there. niagen, c60 and telomerase supplements seem to be the thing right now
2018-12-13.log:01:50 < fenn> even if you are careful and keep the immortalizing genes separate, there's a tiny chance the cell could pick up the telomerase gene from the culture medium
2018-12-13.log:01:55 < fenn> i guess they can screen out cell lines that still have telomerase activity after clonal expansion
2019-07-16.log:09:10 < kanzure> something something telomerase...
2019-08-21.log:06:57 < kanzure> "Transient delivery of modified mRNA encoding TERT rapidly extends telomeres in human cells" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415018/
2019-10-18.log:13:19 < superkuh> Anyway, paper about increasing the physical volume available for pluripotent mouse embryo cells too expand in to and how it causes increased access for the telomerase at that stage resulting in longer telomeres. Then a more recent paper about how this increases life span, https://www.nature.com/articles/s41467-019-12664-x
2019-10-18.log:14:23 < EmmyNoether> Mice with hyper-long telomeres show less metabolic aging and longer lifespans | Nature Communications
2019-11-13.log:18:18 < not_you> Surprised there wasn't any discussion about applying this to human (a real human's, not cells) skin: https://med.stanford.edu/news/all-news/2015/01/telomere-extension-turns-back-aging-clock-in-cultured-cells.html
2019-11-13.log:18:19 < kanzure> did you search the logs or is it just assumed that telomerase-modifying enzymes have never been discussed in here
2019-11-13.log:18:20 < kanzure> er, telomere-modifying and telomerase-modifying
2020-01-29.log:10:45 < kanzure> why do we have like zero telomerase things here https://diyhpl.us/wiki/genetic-modifications/
2020-01-29.log:10:50 < kanzure> yashgaroth: why not just inject genetically modified pre-differentiated telomerase-modified stem cells throughout the body?
2020-01-29.log:10:51 < yashgaroth> stem cells already express telomerase, or do you mean all their descendant cells would also express it?
2020-01-29.log:10:56 < nsh> *telomeres
2020-01-29.log:10:58 < yashgaroth> I feel like accumulated mutations, epigenetic silencing, protein aggregates and dysfunctional mitochondria are at least as responsible as telomeres for aging
2020-01-29.log:11:00 < kanzure> but really, why do we not have telomere stuff on that page
2020-01-29.log:11:02 < yashgaroth> the focus on telomerase in the anti-aging community has annoyed me since they think it alone is the fountain of youth, but we can put some of the more rational research on the page
2020-01-29.log:11:02 < kanzure> i mean there has to be at least one non-stupid thing from the telomerase fanatics
2020-01-29.log:11:03 < yashgaroth> I think inducible telomerase engineered via germline is a lot more sensible than inducible yamanaka factors, which is the latest craze