--- Day changed Sun Oct 12 2008 00:00 < drazak> I wonder how hard it is to synthesize my own 00:01 < kanzure_> Yay 00:01 < kanzure_> http://heybryan.org/mediawiki/index.php/DNA_synthesizer 00:01 < kanzure_> Check the oligonucleotide synthesis instructions. 00:01 < drazak> oh hey 00:01 < willPow3r> i synthesized one with your mom last night 00:01 < drazak> I think I'm going to build my own thermocycler 00:01 < kanzure_> How exactly? 00:01 < kanzure_> I've never understood the rapid cooling mechanisms. 00:02 < drazak> welll 00:02 < kanzure_> Was this some sort of thermal piezo material ? 00:02 < kanzure_> voltage=>rapid cool down methodology? 00:03 < drazak> what I was thinking is instead of using convectiona and radtiation, I'd have a small water heater, and 2 servos to open the gates for a drain and for cooling 00:05 < kanzure_> And what would the physical flow setup look like? a tube sitting next to a pvc pipe with the water flow, or you manually moving the tube from hot to cold water bins? 00:05 < drazak> and put the reagents+thermometer in a mostly covered whatchamacallit (the small plastic 'test tubes' for centrifuges) 00:05 < drazak> no no 00:05 < drazak> no manual moving 00:05 < drazak> I want to be able to walk away 00:05 < drazak> ok, so I have a bin of water 00:05 < drazak> and the ends of the tubes sticking in, the bin of water has an in tube and an out tube, and also a heater 00:06 < kanzure_> What sort of gate do you plan on using? I suppose you could use a metal plate attached to a sturdy pin that rotates like a rotating door. 00:07 * kanzure_ is assuming noncontinuous flow, which probably isn't a good idea. 00:07 < drazak> the heater brings the tubes up the the first temperature, for denaturation, then to cool it, both servos flip, opening the water gates, water flows in until the thermometer hits the temperature for annealing 00:08 < drazak> timer times, heater comes on for extension temperature, and then repeat 30-40 times 00:08 < drazak> the whole process 00:08 < drazak> then it goes to final extension and hold 00:08 < drazak> and we're done 00:11 < drazak> hook it up to either a computer or a programed atmel chip to execute the program 00:11 < kanzure_> I suppose what you really need to do is confirm that the temperatures you hit will do what you think it's doing. 00:12 < drazak> nah 00:12 < drazak> it's well documented 00:12 < drazak> the temperatures, that is 00:12 < drazak> there's a few things that change 00:12 < drazak> but that's simple to change 00:12 < kanzure_> No, I mean your system. 00:12 < drazak> oh 00:12 < kanzure_> confirmation of functionality and such. 00:12 < drazak> yeah 00:12 < drazak> which is why you have a therister in one of the tubes, measuring the temperature of the reagents 00:13 < drazak> so that you can ignore specific heat 00:15 < kanzure_> fenn: How does the AA heat the water in the $10 thermocycler? 00:15 < drazak> hm 00:15 < drazak> you know 00:16 < drazak> it would be simpler to simple turn on 2 water pumps 00:16 < drazak> than fuck with servos 00:16 < drazak> you can get cheap water pumps for like $2-5 each 00:16 < kanzure_> is that so? 00:16 < drazak> yeah 00:16 < drazak> really small ones 00:16 < drazak> but you don't need very big 00:16 < kanzure_> if you can price out a system for less than $10, I can hook you up with a manufacturing deal 00:16 < drazak> well 00:17 < drazak> it's going to be ~15 00:17 < drazak> off the top of my head 00:17 * kanzure_ waves hand in crude attempt at Jedi mind trick, "You want to price it out for under $10." 00:17 < drazak> you could do it with cheap paint trays 00:17 < drazak> yeah yeah 00:17 < kanzure_> right 00:17 < drazak> I'm just thinking of how to do it cheaper 00:17 < drazak> so you need 3 paint trays 00:18 < drazak> so there's like 3-4 bucks 00:18 < drazak> I guess you could do it with one water pump 00:18 < drazak> so call it 2 bucks 00:18 < drazak> the water heaters the only expensive thing 00:18 < drazak> I was thinking one for fish tanks 00:18 < drazak> which is like 10-15 00:19 < kanzure_> what's so special about this heater? 00:19 < drazak> dunno 00:19 < drazak> thinking about that more 00:19 < drazak> that's a bad idea 00:19 < drazak> because you'd have to rewire it so that it goes hotter 00:27 < kanzure_> Hrm. I used to blow shit up all the time when soldering. Stuff would heat up without warning. 00:27 < kanzure_> Find a high emissivity resistor to wire up to a battery, hook that into a slightly larger test tube and submerge the other tube within that one as well. 00:28 < kanzure_> Water is still good, though maybe not the water heater ;-) 00:32 < drazak> heh, I know how to solder rather well 00:32 < drazak> I design and build my own headphone amps 00:35 < kanzure> It's not a matter of soldering well :-) but rather doing dangerous things with what you're soldering. 00:35 < drazak> nahh 00:35 < drazak> not if you hit it fast 00:35 < drazak> there's a technique behind soldering stuff like that 00:36 < kanzure> No, it's not the soldering that does the Dangerous Thing, it's what you're soldering together that blows up when you turn it on or whatever. 00:36 < drazak> oh, that 00:36 < drazak> :P 00:36 < drazak> thanks knowing electronics 00:36 < drazak> :D 00:36 < kanzure> Hrm? 00:37 < drazak> anyway 00:37 < drazak> it looks like the cheapest water pump online is like 10$ 00:37 < drazak> I swear I bought some for like 3 or 4 though 00:39 < fenn> the idaho thermocycler i used the crap out of was just a fan and a lightbulb (and a microcontroller) 00:39 < drazak> hm 00:39 < fenn> it's the fastest design, because instead of microcentrifuge tubes you use glass capillary tubing, so it has a large surface area to volume ratio 00:40 < drazak> well 00:40 < drazak> water+water heater is faster 00:40 < drazak> get a heater and turn up the power, and you'll be doing good 00:40 < fenn> i think you're wrong about that 00:41 < fenn> water heats by conduction, whereas a lightbulb can heat by IR radiation 00:41 < fenn> the pcr mix had a blue dye in it, which might have absorbed IR but i think it was just so you could see what you're doing 00:42 < drazak> yeah 00:42 < drazak> the blue dye is actually to help with the gel 00:42 < drazak> because it's nto a dye at all 00:43 < drazak> it's bromophenol blue 00:43 < drazak> which is a loading buffer 00:44 < drazak> basically when you're doing the gelling, it moves at the same speed as the dna so that you can see when it's done 00:44 < fenn> btw domestic water heaters have this valve that prevents it from getting hot enough for pcr 00:45 < drazak> oh, that's not the type of heater I was talking about 00:45 < fenn> ok 00:45 < drazak> I meant like a fishtank heater 00:46 < fenn> i like the lightbulb+fan idea 00:46 < fenn> twenty seconds on, twenty off, vroom vroom vroom 00:47 < drazak> eh 00:47 < drazak> I guess 00:47 < drazak> I'd use one of those special bulbs though 00:47 < drazak> or like a 100W bulb 00:47 < fenn> 100W is special? 00:47 < drazak> or 00:47 < drazak> :D 00:48 < drazak> but they make special bulbs for heating stuff 00:48 < fenn> oh, sure 00:48 < fenn> you can get 130V "commercial service" bulbs too, which glow a little dimmer 00:48 < fenn> more IR in the spectrum 00:48 < fenn> not that it matters i guess, since all the light gets converted to heat eventually 00:52 < drazak> what microprocessor? 00:52 < drazak> I can't think of any you can buy for that cheap 00:54 < kanzure> PICs are ridiculously cheap 00:54 < drazak> are they? 00:54 < kanzure> buck fifty? 00:54 < drazak> I don't like the PICs as much as the atmel ones 00:54 < drazak> avr's 00:54 < kanzure> well sure .. but those are going to cost more. 00:55 * drazak opens his tin of parts 00:55 < drazak> hmm 00:56 < drazak> I see like 5 or 6 avrs 00:56 < kanzure> screw it 00:56 < kanzure> just hook it up to LTP 00:56 < drazak> yeah 00:56 < drazak> I was thinking about that 00:56 < kanzure_> or RS232 00:56 < kanzure> or RS232. 00:56 < kanzure_> shut up. 00:56 < drazak> I can't program though, so regardless, someone else is going to have to help me write the program 00:56 < drazak> :P 00:57 < kanzure_> Highly dependent on how friendly your inputs are to the machine. Does the thermometer have a clear protocol for spitting back the current temperature? etc. 00:58 < drazak> if you use a thermistor 00:58 < kanzure_> if it's wired to an LCD, then the answer is yes, and just remove the LCD and wire it into your boxen 00:58 < drazak> you can just use an a/d converter 00:58 < kanzure_> ? 00:58 < kanzure_> analog to digital converter on /what/ ? 00:58 < drazak> a thermistor 00:58 < kanzure_> How does this work? 00:58 < kanzure_> surely you don't mean an analog read out to digital ? 00:59 < drazak> a thermistor is basically a resistor that changes resistance based on temperature 00:59 < kanzure_> oh. nevermind. 00:59 < drazak> :P 01:13 < kanzure_> Somebody's sending me storage for a few dumps of my collections. 01:14 < kanzure_> I'm planning on first sending it somewhere else. Who wants? 01:15 < drazak> your music? no thanks 01:15 < kanzure_> It's not music. 01:16 < kanzure_> I'm fairly confident that I have a highly desirable collection over here. 01:16 < drazak> porno? 01:16 < kanzure_> nature 01:16 < drazak> oh :P 01:55 < fenn> atmel AVR's are $0.50-$15 01:55 < fenn> last time i looked at least 01:56 < fenn> the $2 ones will do just about anything you can ask of a microcontroller 01:57 < fenn> PIC gets a reputation for being cheap because their company will send free samples to anybody no questions asked 01:58 < fenn> how big is nature? 01:58 < kanzure_> 40 GB but it seems I'm missing some things. For instance, I didn't have that Raman paper. 01:58 < fenn> ookina yu-ni-va-su 01:58 < kanzure_> ? 01:59 < drazak> kanzure_: what's in nature? 01:59 < kanzure_> the majority of nature 02:00 < kanzure_> I have an 8 MB index if you want to see it 02:00 < drazak> do you have any sort of access to published papers/whatnot? 02:00 < drazak> that I could use 02:00 < kanzure_> ha ha ha 02:00 < kanzure_> yessir 02:01 < drazak> :D 02:01 < drazak> that'd be awesome 02:01 < fenn> will probably hate this: http://fennetic.net/pub/irc/coppe_8_monologue.mp3 02:01 * kanzure_ has been listening to Carlos Santana - Europa for the past I don't know how long 02:02 < kanzure_> http://heybryan.org/papersdir_list.txt warning, this is about 8 MB. 02:02 < drazak> how big is the directory with the actual papers? 02:03 < fenn> how many papers? 02:03 < kanzure_> 122,000 papers 02:03 < kanzure_> checking with du -h. 02:04 < drazak> kanzure_: du -ckhs is more efficient, fyi 02:04 < kanzure_> hm. Maybe I meant to upload full_index.txt 02:05 < fenn> du -k and du -h cancel, -k wins? 02:05 < fenn> and -c -s doesnt make sense either 02:05 < drazak> uh 02:06 < drazak> fenn: k doesn't do what you think it does 02:06 < drazak> :D 02:06 < fenn> k reports number of 1k blocks 02:06 < fenn> which screws up -h 02:06 < drazak> wrong! 02:06 < drazak> well 02:06 < drazak> right 02:06 < fenn> enlighten me then, O master of vudu 02:06 < drazak> but h takes preference 02:06 < drazak> hm 02:06 < drazak> using an outdated command 02:07 < fenn> du -chks 02:07 < fenn> 9828 . 02:07 < fenn> 9828 total 02:07 < drazak> that's how you'd do it for coreutils like 9 versions ago 02:07 < drazak> lulz 02:07 < drazak> not on my system 02:07 < drazak> drazak@localhost ~/My Music/NIN $ du -ckhs 02:07 < drazak> 2.2G . 02:07 < drazak> 2.2G total 02:07 < kanzure_> 130+ GB of music if that counts. Still running. 02:08 < drazak> regardless though, if you want to know how big a directory is, du -ckhs is better, it doesn't print/try to print to standard out if you have a whole bunch of papers 02:11 < kanzure_> Still going. 02:11 < kanzure_> It's not listing files yet either. 02:12 < kanzure_> Had I been smarter I would have preserved some directory structure to the rip. 02:13 < fenn> like volume, issue? 02:13 < kanzure_> yes 02:13 < fenn> what are the filenames like? 02:14 < kanzure_> title of the paper 02:14 < drazak> kanzure_: I was hoping some sort of university login or something 02:15 < kanzure_> drazak: You want me to give you logins? Sorry, I don't do ezproxy trading rings any more .. I tried to trace them to the source once, but that failed. 02:15 < drazak> not what I meant 02:15 < fenn> "00 million pledged to support ex-Soviet science" how generous 02:15 < drazak> but ok! 02:17 < fenn> there's gotta be some way to "slice" huge datasets into different file systems without duplicating the data 02:17 < kanzure_> 'file systems', split 40 GB into ext2 and ext3 ? 02:18 < fenn> i mean so you can have a big dir full of every paper, and also sorted by volume/issue, and also by field or whatever 02:18 < fenn> and not have to use some crappy SQL server to do it 02:18 < kanzure_> something better than virtual links? 02:18 < fenn> links would work i guess 02:19 < drazak> kanzure_: why would you split it into ext2 and 3? 02:19 < drazak> the only difference is journaling 02:19 < kanzure_> trying to understand fenn. 02:19 < drazak> and ext3 generalyl speaking gives better performance 02:19 < kanzure_> he said 'file system' 02:19 < kanzure_> so I thought 'file systems'. 02:19 < drazak> oh :P 02:19 < fenn> i meant directory structure 02:19 < fenn> i was thinking " 02:19 < fenn> proc filesystem 02:20 < drazak> lol 02:20 < fenn> why is that funny? 02:20 < fenn> nevermind, trying to explain why something is funny is an exercise in futility 02:23 < drazak> aye 02:25 < fenn> kurzweil has this music generator program that responds to biofeedback 02:25 < fenn> it would be neat to do that with a chatroom 02:25 < kanzure_> streaming audio server? 02:26 < fenn> often i want to beam music phrases at people but 1) english sucks at that and 2) you'd disrupt whatever the other person was listening to or just the mood in their head 02:26 < kanzure_> recently I was thinking of a program that would generate european dance music mixes 02:26 < kanzure_> it's fairly algorithmic to get a beat going, plus plays on samples taken from a library of various snippits of classic songs 02:26 < fenn> as much as i like euro trance i think that's an awful idea 02:26 < kanzure_> heh 02:27 < fenn> sort of like a markov bot, but with music 02:27 < kanzure_> sir, are you saying I'm a bad idea? 02:27 < fenn> it's entertaining for about ten minutes 02:27 < kanzure_> people have called me a markov bot before. 02:27 < kanzure_> aw. 02:27 < fenn> nothing personal :) 02:27 < fenn> its just sometimes you show an extreme lack of physical groundedness 02:28 < fenn> like the recent discussion on openmanufacturing 02:28 < drazak> aye 02:28 < kanzure_> arguably I'm more grounded 02:28 < drazak> wellll 02:28 < kanzure_> note how I've been talking about interfacing with B2B for the material procurement issues 02:28 < drazak> as much as it's ac ool idea, I think that most of the hplus research can't be done in an opensource enviroment 02:28 < kanzure_> you get your direct realization of materials from bits and bytes, what's not to love about that ? 02:29 < drazak> nto easily, atleast 02:29 < kanzure_> drazak: eh? 02:29 < fenn> i think he was talking about more of an autonomous ground-up scheme 02:29 < kanzure_> explain? 02:29 < kanzure_> fenn: patrick? 02:29 < fenn> rather than trying to interface with businesses etc, just make what you need to spec 02:29 < fenn> yeah 02:29 < kanzure_> fenn: I don't know why he doesn't call that a design. 02:29 < drazak> kanzure_: well, most of the research takes a lot of money to do it 'right' 02:29 < kanzure_> "it's not a design! it's reality! rawr" 02:29 < drazak> and if we're not going to do it right, we may as well not do it at all 02:30 < kanzure_> drazak: Do what, now? 02:30 < fenn> because his vision is exceedingly low-tech and based on tested designs mostly, and he has no idea how to actually implement it (business plan stuff) 02:30 < fenn> it's easy to come up with a business plan when you have a technological advantage, but not so easy when you're digging in the dirt like a medieval subsistence farmer 02:30 < drazak> kanzure_: research bioengineering of the human race in a way that could be applied large scale 02:31 < kanzure_> is that your understanding of what we've been talking about, drazak? 02:31 < fenn> and so i'm sort of amazed my friend just got a $100k loan to dig in the dirt in bumfuck pennsylvania 02:31 < drazak> kanzure_: well, there's more to it 02:31 < kanzure_> fenn: building a house? 02:31 < fenn> farming 02:31 < drazak> but imo to do the research, you may as well do it right, and I'm all for finding out a way to come up with a low cost solution 02:32 < kanzure_> solution to what? 02:32 < drazak> solution to research tools 02:32 < kanzure_> You're being vague :-) 02:32 < drazak> aye 02:32 < fenn> 'solution to research tools' wtf does that mean 02:33 < fenn> like "how to do PCR without paying invitrogen $50/pop"? 02:33 < kanzure_> fenn: but he was talking about overall goals and projects, 02:33 < kanzure_> so I'm wondering what he thinks those are 02:33 < drazak> solution to lack of research tools/supplies 02:33 < kanzure_> human race engineering or whatever seems like a peculiar answer 02:33 < kanzure_> it's not a wrong answer, just something I wanted to poke at 02:34 < drazak> it's part of the impression I got from the biohack site 02:34 < fenn> in the history of science, there was no supplier that magically created analytical tools out of thin air 02:34 < fenn> people had to come up with their own designs and build them with what they had 02:35 < fenn> now we just have a big patent snarl 02:35 < drazak> fenn: aye, but most of the tools needed for research in molecular biology and whatnot have already been made 02:35 < drazak> they're just expensive 02:35 < fenn> time to move to guptastan I say 02:35 < kanzure_> drazak: yes, a big part of Things in general is bootstrapping tools. 02:38 < fenn> i find it odd that smari is whining about people bringing in unidentified plastic, but nobody is working on fablab-DIY chemical analysis tools 02:38 < fenn> what the heck do they make in these fablabs if not better fablab tools? 02:38 < kanzure_> in the cities and the US, I don't know 02:39 < kanzure_> but supposedly Gershenfeld has these in third world countries 02:39 < drazak> kanzure_: I get the impression that transhumanism is about making humans and therefor humanity better 02:39 < kanzure_> and theoretically they're making 'useful stuff' ? 02:39 < fenn> useful stuff is like, radio transponders for sheep, or milk dilution testers 02:39 < kanzure_> drazak: btw, you can ignore the title of the channel. that's leftovers because everybody keeps showing up here. 02:39 < kanzure_> drazak: but yes, that'd be nice. 02:40 < fenn> gershenfeld goes on and on about "the analytical divide" but its in his own labs if you ask me 02:40 < drazak> then what is the general goal of the channel 02:40 < fenn> the same goal of every channel 02:40 < fenn> ... to try and take over the world! 02:41 < fenn> it's true, dont try to deny it 02:41 < willPow3r_> dan dennett is so jittery when he speaks 02:41 < willPow3r_> its hard to follow what he says sometimes 02:42 < kanzure_> It would be interesting but pointless to say the goal is improvement. 02:42 < drazak> aye 02:42 < kanzure_> I'm not sure we can prescribe a goal 02:44 < fenn> based on the channel name i think the idea is to come up with a way to get to various types of improved states of being 02:44 < fenn> way/ways 02:44 < fenn> for instance, i want to see in radio to x-ray spectrum 02:45 < drazak> no thanks 02:45 < fenn> so that's part of why i'm obsessed with wearables 02:45 < kanzure_> I'm not sure if we can go by the channel name any more. 02:45 < fenn> kanzure_: too "mission oriented" for you? 02:46 < fenn> at least we arent talking about taxes or communism 02:46 < drazak> I'm trying to come up with a goal for my own research 02:46 < drazak> that's acheivable in ~6 months 02:46 < fenn> come up with a goal that's not achievable in 6 months 02:47 < fenn> then try to do something related 02:47 < fenn> you might even get tenure if you repeat that enough times 02:47 < drazak> heh 02:47 * fenn sulks 02:47 < drazak> you do know I'm 15, yeah? 02:47 < drazak> s/15/16 02:47 < kanzure_> too old. 02:47 < fenn> don't pigeonhole yourself 02:48 < fenn> the only reason young people don't play a more active role in society is they get sent to sensory-deprivation prisons for 15 years 02:48 < drazak> fenn: this is why I'm trying to teach myself a whole bunch of stuff :) 02:48 < drazak> I worked my way through ochem 1 and 2 recently 02:48 < drazak> learning biochemistry atm 02:49 < kanzure_> fenn: so, yes, it does sound almost too mission oriented. 02:49 < kanzure_> recall the issues in recursive self-improvement. 02:50 < fenn> kanzure_: do you not believe in the concept of "improvement" now? 02:50 < kanzure_> people still trying to figure out how to quantify improvement 02:50 < fenn> we can very easily quantify some types of improvement 02:50 < fenn> heck there's even a world happiness index 02:50 < kanzure_> I could possibly go for some computational optimization big o notation arguments on certain types of improvements. 02:51 < fenn> 0 cpu cycles is the best kind of algorithm 02:51 < fenn> (translation: people do a lot of useless crap) 02:51 < kanzure_> O'Neill habitats, or even O'Neill habitats without the "in space" aspect, is where it's at -- it's not a platform necessarily for improvement, but if improvement or beneficial mutation does occur, it can be harnessed and repeated. 02:52 < fenn> luf is all about the ocean habitats, and the more i think about it the more i realize that the moon would be a lot easier to colonize 02:52 < kanzure_> fenn: easier than the ocean? 02:52 < fenn> (if only we had a rotovator) 02:52 < gene> rotovator? 02:52 < fenn> well, two rotovators, one for earth and one for luna 02:52 < gene> oh yeah 02:52 < gene> I remember now 02:53 < fenn> momentum exchange tethers 02:53 < gene> you might be able to dig a hole in the moon if you get your lunar rotovator just right 02:53 < fenn> i think the objective is to not crash into the moon :) 02:54 < gene> but if you but a shovel on one end ans spin it fast enough... 02:54 < fenn> just fill plastic bags with the loose dust that's everywhere on luna 02:54 < gene> yup 02:54 < fenn> then the tether comes down and picks up the bag 02:54 < gene> and sell it on earth as genuine moon dust! 02:55 < fenn> hah, sell it in orbit as genuine space colony materials 02:55 < gene> that too 02:55 < fenn> or use it to re-boost your earth rotovator 02:55 < gene> yeah 02:56 < gene> or shoot it at the earth's atmosphere for a continuous fireworks show 02:57 < fenn> most people dont realize that lava tubes are kilometers in diameter on luna 02:57 < gene> KILOMETERS? 02:57 < fenn> yep 02:57 < gene> you're kidding 02:57 < gene> damn 02:57 < fenn> low gravity 02:57 < gene> great place to build a house 02:59 < fenn> you can see some collapsed tubes, um, hadley rille maybe is one of them? 02:59 < kanzure_> fenn: so, back on topic 02:59 < kanzure_> where were we. 02:59 < kanzure_> were we making me declare a goal or something? 03:00 < drazak> maybe 03:00 < drazak> :D 03:00 < fenn> i think you were trying to clear out the cruft accumulating in the topic 03:00 < kanzure_> in the /topic ? 03:01 < gene> goal 03:01 < gene> to make a self-replicating automaton 03:01 < bkero> I'll tubes your topic. 03:01 < kanzure_> gene: That's not the meta-goal. 03:01 < kanzure_> gene: The kinematic self-replicating machine work is a subset of some others. 03:02 < gene> please specify meta goal 03:02 < kanzure_> fenn: feel free to moderate the /topic I guess. 03:03 < kanzure_> Everyone has known my own personal goals for a while now .. that simple "to do as much as possible" phrase. 03:03 < gene> specify goal 03:03 < gene> to make a replicator validator program? 03:03 < fenn> self replicating machines are useful for effectively eliminating most economic costs 03:03 < fenn> but then what to do with them? 03:04 < kanzure_> fenn: That's not the way that we stumbled upon them. 03:04 < gene> what do you mean what to do with them 03:04 < fenn> i want to build giant structures in space to communicate with the zodiac, but i dont expect anyone else to share that goal 03:04 < gene> solve world hunger, make dyson spheres, make cheesecakes the size of cities 03:04 < fenn> yes, cheesecakes, the size of cities 03:05 < kanzure_> The reason why I refuse to specify goals so clearly any more is because of my previous involvement in that goal cult. 03:05 < fenn> kanzure_: it's the way i stumbled upon them 03:05 < fenn> kanzure_: have you seen the price tag on a new lathe? 03:05 < kanzure_> fenn: In our discussions in here, specifically, we started from talk about things other than replicators, and then arrived at replicators. That's what I meant. 03:06 < kanzure_> Wait. Maybe not. /me doesn't know his history. 03:06 < fenn> hm the new lathe argument doesn't work so well with all the cheap tools from china now available 03:06 < kanzure_> Argument for what? 03:06 < fenn> but they used to be like $8000 03:06 < fenn> so it was worth taking the time to build one from scratch 03:06 < fenn> and then i realized that there was just all this stuff laying around, and you just had to put it together right 03:07 < fenn> but the lathe is mostly self-generating, so how far could you stretch that concept 03:07 < kanzure_> fenn: I seem to recall us moving to talks about replicators after talk about 'improvement'. 03:07 < gene_> what the heck just happened 03:07 < fenn> i seem to recall you found me because of the gingery machines wiki 03:08 < fenn> or something like that 03:08 < kanzure_> hrm, that's right. 03:08 < kanzure_> So maybe it was just replicators from the go. Huh. 03:08 < fenn> anyway, i was working on airmuscles for an exoskeleton or some such 03:08 < fenn> so it's not like i was just pimping my chevy 03:10 < drazak> hm 03:11 < kanzure_> drazak: This was recent and might point to a few things: http://heybryan.org/bootstrapping_personal_fabrication.html 03:12 < kanzure_> I sent an email to Michel once that talked about 'supply chain length minimization' (I am program, programmer and that which is programmed) 03:13 < kanzure_> That phrase has relations to http://heybryan.org/recursion.html 'course. 03:15 < drazak> hm 03:15 < drazak> I need to learn more about synthesizing primers 03:15 < kanzure_> http://heybryan.org/fractal.html was a partial elaboration about springboarding via philanthropical bootstrapping to get to stable points of functionality in large scale systems (like, say, O'Neill habitats, or civilizations in the sense that Kevin Kelly talks about re: civilizations-as-an-organism) 03:16 < kanzure_> drazak: http://primerfox.com/ 03:16 < drazak> I wonder if there's a way eventually to synthesize bacteria to make commonly used primers 03:16 < kanzure_> http://heybryan.org/mediawiki/index.php/DNA_synthesizer for oligonucleotide synthesis instructions 03:16 < gene_> FURRY ALERT 03:16 < kanzure_> drazak: Yes, there is. 03:16 < kanzure_> drazak: Consider wrapping the primers in the plasmid or bacterial genome with endonuclease recognition sites. 03:17 < kanzure_> And then just have bacteria that manufacture the endonucleases, and purify those. 03:17 < gene_> what's a primer? 03:17 < kanzure_> That's the idea of http://heybryan.org/new_exp.html the do-it-yourself bioreactor project. 03:17 < drazak> not wha I was thinkig at all 03:17 < drazak> hold on 03:17 < kanzure_> gene_: http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR requires primers so that polymerase knows where to start replicating. 03:17 < drazak> let me grabthe word 03:18 < kanzure_> s/replicating/copying/ 03:18 < fenn> oh my god, your nick is "gene" and you dont know what a primer is? 03:18 < gene_> I thought primers were the nucleotides not the sequence you want to copy 03:18 < drazak> ahh, plasmid is the word 03:18 < drazak> yeah 03:19 < drazak> kanzure_: plasmids would definitely be a way to do it, I'd think 03:19 * fenn is cranky, must be time for bed 03:19 < kanzure_> Sure, but you still need to have a supply of endonucleases. 03:19 < kanzure_> fenn: but we're not done 03:19 < drazak> kanzure_: are plasmids replicated in bacterial cell division? 03:20 < kanzure_> drazak: Sort of. I recall that they 'drop off'. They are not multimeiosis-cyclic-stable. 03:20 < kanzure_> Erm. 03:20 < drazak> hm 03:20 < kanzure_> fenn etc - are you guys really going to hold me for a 'goal' ? 03:20 < drazak> that's a flawed way to do it then 03:21 < fenn> i like google's "dont be evil" 03:22 < fenn> but that's not a goal 03:22 < kanzure_> whatta about my energy useage maximization principle? 03:22 < kanzure_> "Do as much as possible with as little as possible, until I can do everything with nothing at all" 03:22 < fenn> why would you want to use energy? 03:22 < fenn> right 03:22 < gene_> I think you mean minimization 03:22 < kanzure_> No, I do not mean minimization. 03:22 < kanzure_> No point in being idle. 03:22 < gene_> ok 03:22 < gene_> I see what you mean 03:23 < fenn> bucky fuller called this technological growth process 'ephemeralization' 03:23 < kanzure_> and Max More calls it 'entropy' 03:23 < kanzure_> erm 03:23 < kanzure_> 'extropy 03:23 < kanzure_> Fuck. 03:23 < fenn> the word extropy is too bound up in philosophy 03:24 < fenn> cell phones are generally not considered "extropic" are they? 03:24 < gene> whoa 03:24 < kanzure_> I don't know about that generally, but I would consider them extropic, yes. 03:24 < gene> something weird is going on 03:25 < fenn> your connection is flaky 03:25 < gene> I keep losing my connection to the irc server 03:25 < gene> or my kinematic modellor could be messing it up 03:25 < kanzure_> Lots of relations to the constructal theory too -- the points of suboptimization are construed to the nodes in the system, i.e. cell phone users, see how much they hate their interfaces (little keys, not a lot of memory, and yet ..). <- I'm screwing this up a bit, but I'm too lazy to figure out how at the moment. 03:26 < kanzure_> Something about natural systems organizing such that all of the choices that you'd rather not make are made by the individual agents, i.e. an individual router ends up having to choose where to send packets to instead of global routing protocols. 03:26 < kanzure_> Anyway. 03:26 < fenn> stigmergy 03:26 < gene> just do it 03:27 < gene> damn 03:27 < gene> kinematic modeller isn't liking my geometry at all 03:28 < drazak> kanzure_: I meant for making my own primers chemically 03:28 < drazak> kanzure_: as in building the sequence 03:28 < kanzure_> Yes, that's nucleotide synthesis via oligonucleotide synthesis. 03:29 < kanzure_> Oh, do you mean to start with pre-made dNTPs? 03:29 < drazak> no 03:29 < gene> what about the nucleotides? 03:29 < fenn> start with nitrogen, oxygen, carbon, phorphorus, hydrogen? 03:30 < drazak> fenn: not... really 03:30 < fenn> or maybe just a big ball of undifferentiated energy 03:30 < drazak> fenn: synthesis of nucleotide chains 03:30 < kanzure_> I'm pretty sure the oligonucleotide synthesis stuff works for that, drazak. 03:30 < drazak> ok 03:31 < drazak> yep, didn't know it was a different word 03:31 < drazak> :) 03:31 < fenn> standard oligo stuff is only good for 25-50bp; how do they do those 3kbp sequences? 03:31 < kanzure_> ligation? 03:32 < gene> Deinococcus radiotolerans DNA repair complex? 03:32 < gene> use sticky ends? 03:32 < fenn> but you'd have the same error rate, unless they somehow amplified single strands and checked each one 03:32 < kanzure_> I doubt they check. 03:32 < fenn> it's not damaged dna, you just get the wrong sequence 03:33 < gene> that's what the Deinococcus radiotolerans DNA repair complex does 03:33 < fenn> they'd have to check, otherwise they'd have the same error rate as if they just made a big oligo from scratch 03:33 < gene> it puts two sticky ends together 03:33 < gene> if some of the nucleotides don't match then it replaces them 03:33 < fenn> gene: this is single strand synthesis 03:33 < kanzure_> gene: That's not what we're talking about. 03:34 < kanzure_> http://mrgene.com/ 03:34 < kanzure_> http://e-oligos.com/ 03:34 < gene> yeah 03:34 < fenn> there is no other strand to check against 03:34 < gene> but you can synth a bunch of small strands such that they all overlap 03:34 < gene> and then have the repair complex check em 03:34 < gene> venter's doing it 03:35 < kanzure_> The repair complex checks complementary strands. 03:35 < gene> to compile his organisms 03:35 < kanzure_> There is no complementary strand here. 03:35 < fenn> gene: do you have a link explaining this process? 03:35 < gene> fuck wrong bacteria 03:36 < gene> I meant to say deinococcus radiodurans 03:36 < fenn> that's how i read it anyway 03:37 < gene> http://en.wikipedia.org/wiki/Deinococcus_radiodurans 03:37 < kanzure_> gene: On Monday I can give you a linux disc. 03:37 < gene> good 03:37 < gene> vista has pissed me off 03:38 < gene> so deinococcus checks it genome against multiple copies 03:38 < kanzure_> Yes, so that's definitely not what we're talking about. 03:39 < gene> then what are you talking about single strand synthesis? 03:40 < fenn> i can see how this would work, since you get to 'vote' on the correct nucleotide 03:40 < gene> yeah 03:40 < gene> I think it's sorta like that 03:41 < gene> I haven't read many radioresistance papers in a while 03:41 < fenn> i guess i should just watch venter's ted talk instead of fighting with google 03:41 < gene> nah 03:41 < gene> I don't think venter says much about it in the ted talk either 03:42 < gene> I believe he just puts the dna in deinococcus 03:42 < gene> or something like that 03:43 < fenn> well here's a protocol, doesnt really answer my questions: http://books.google.com/books?id=8x02velGjKMC&pg=PA374&lpg=PA374&dq=overlapping+oligo+synthesis&source=web&ots=dCVC6hCYB8&sig=jY18FQL4BEE-VCKtFtQn3wCm2H8&hl=en&sa=X&oi=book_result&resnum=5&ct=result 03:44 < fenn> 'screening isolates by cloning and sequencing' is basically what i meant by having to check each oligo 03:44 < kanzure_> right 03:45 < fenn> 450 is much better than 50 03:49 < gene> so how do we synth enough to make a protein 03:49 < gene> like a fancy polymerase 03:50 < kanzure_> what are you on about now 03:50 < fenn> matter compiling 03:50 < gene> heh 03:50 < fenn> chicken and egg problem 03:50 < gene> you've read The Diamond Age haven't you fenn 03:50 < fenn> yep, but i had the idea first :P 03:52 < fenn> so you just repeat the 'clone and sequence (and ligate)' process until you have a long enough sequence 03:52 < gene> guess so 03:52 < fenn> you can do lots in parallel of course 03:52 < fenn> so all the next-gen sequencing stuff hleps 03:54 < drazak> I think I need to read more, to understand more for all of this 03:54 < fenn> mumble mumble microfluidics array grumble 03:54 < kanzure_> drazak: this? 03:54 < drazak> kanzure_: not /this/ 03:54 < drazak> but biochemistry in general :P 03:54 < kanzure_> then that? 03:54 < kanzure_> oh 03:54 < drazak> I'm gonna grab my text and a soda and dig in :P 03:54 < gene> you can make microfluidics with shrinky dinks 03:55 < fenn> so they say 03:55 < fenn> i mean, um, that's what she said, or something 03:55 < gene> high quality microfluidics 03:55 < gene> that don't have metal 03:55 < gene> unfortunately 03:56 < fenn> metal? 03:56 < gene> for pumping 03:57 < fenn> can't you just squeeze them? :) 03:57 < gene> no 03:58 < gene> not if you want to do it fast 04:01 < fenn> ultrasonics + valvular conduits 04:01 < gene> maybe 04:01 < fenn> magnetorhelogical fluid 04:02 < fenn> magnetohydrodynamic thrusters! 04:02 < gene> then you have ions 04:02 < drazak> magnetobacteria! 04:02 < gene> hmmm 04:02 < gene> you mean like bacto ferrofluid? 04:03 < fenn> "Magnetobacteria are gram-negative proteobacteria that use flagella to swim." 04:04 < drazak> aye 04:04 < drazak> they're also anaerobic, iirc 04:04 < fenn> caulobacter have this anchor thing to stick to any surface, and they also have flagellae 04:04 < gene> and have tiny magnetite magnetosomes 04:04 < fenn> so you could use some mutant caulobacteria as a cilium 04:04 < gene> then you have contamination 04:05 < gene> but it is an interesting concept 04:07 < fenn> i feel a weakness coming on 04:13 < gene> did you accidently a whole bottle of coke? 04:14 < willPow3r_> omg 04:14 < willPow3r_> /b/tard 04:14 < gene> no 04:14 < gene> I don't go to /b/ 04:14 < gene> I never go to b 04:16 < willPow3r_> neither do i 04:16 < gene> good 04:17 < gene> never goto /b/ 04:17 < willPow3r_> because goto is bad programming practice 04:20 < willPow3r_> b/tard :Unknown comman 04:20 < willPow3r_> xchat is funny 04:38 < ybit> "[Sat Oct 11 2008] [19:05:33] ok, so I have a bin of water" -- last log saved until someone in the family wanted to play around on here while i was showering. yes, i bathe 04:38 < drazak> haha 04:38 < ybit> so, anyone mind linking to a logfile maybe? 04:42 < bkero> http://www.boingboing.net/2008/09/23/what-went-wrong.html 04:53 < drazak> hm 04:53 < drazak> it seems most of these methods 04:54 < drazak> are for large scale oligonucleotide synthesis 04:55 < ybit> so, nothing interesting? 04:55 < ybit> or nothing at all... 05:12 < kanzure_> ybit lots has happened. 05:12 < kanzure_> unfortunately, I'm not logging from this box 05:16 < ybit> oOokay 05:17 < gene> hey one of the oligosynth websites says you get a free gift with every order 05:17 < gene> wonder what it could be? 05:17 < gene> coupons? 05:17 < gene> Centrifuge? 05:18 < gene> enzymes? 05:18 < gene> ep tubes? 05:18 < gene> guess we'll never know 05:19 < gene> cus' we're doing it ourself 05:40 < kanzure_> http://web.archive.org/web/20060615181106/http://www.bizdex.com.au/ "National interoperability framework" holy shit I'm moving to Australia. 05:49 < kanzure_> Hey, trade tariffs on unmetadatetized materials. 05:52 < kanzure_> better yet, trade tariffs on companies that refuse to do the whole open UDDI B2B ebXML trick 05:58 < gene> SWEET 05:58 < gene> hey kanzure is there any way I can use my laptop as a display for another computer 05:59 < kanzure_> Desktop-over-the-network, rewire the LCD, probably a few other methods I'm forgetting. 05:59 < gene> damn 06:00 < gene> I ain't rewiring this laptop 06:00 < gene> it's new 06:00 < kanzure_> Then do network-over-the-desktop. 06:01 < gene> nevermind 06:03 < gene> so could you help convert me to linux 06:03 < gene> on monday? 06:04 < gene> the hard drive partitioning is the part I am not familiar with 06:05 < willPow3r_> gene, tried vnc? 06:05 < gene> no 06:05 < kanzure_> Partitioning is done by the installation wizards, but first you should run 'disk defragmentation' so that you don't have files in weird places. 06:06 < gene> good 06:06 < kanzure_> Anyway, it's a good idea to do a full backup of anything important. 06:06 < gene> ok 06:06 < gene> that's easy 06:06 < gene> I don't have much important stuff 06:06 < gene> beside programs 06:07 < gene> the thing that really annoys me about vista right now is that solidworks will try to start up at some random time 06:07 < gene> but it can't 06:07 < drazak> So, I really want to research gap junctions and stuff 06:07 < drazak> but I'm nowhere near equiped to do that 06:07 < gene> so it crashes 06:07 < gene> what are gap functions? 06:08 < gene> programming related or biology or other? 06:09 < gene> oh 06:09 < gene> biology related 06:09 < drazak> gap junctions are how certain cells can preform a 'docking' process and exchange fluids 06:09 < drazak> it's done with connexin proteins 06:09 < gene> oh 06:09 < gene> that's definately cool stuff 06:09 < drazak> aye 06:09 < drazak> a teacher at my highschool did 1.5 years of research on it 06:09 < gene> get a bunch of cells to link up 06:09 < drazak> before he lost funding 06:09 < drazak> wellll 06:10 < drazak> it's a lot more complicated than that 06:10 < drazak> ;) 06:10 < gene> make a circulatory system of sorts 06:10 < drazak> nope 06:10 < gene> of sorts 06:10 < drazak> doesn't work like that 06:10 < gene> ok 06:11 < gene> dna exchange? 06:11 < gene> like in bacteria? 06:11 < gene> or are you talking about something in eukaryotes 06:11 < drazak> http://www.jbc.org/cgi/content/abstract/277/39/36725 06:11 < drazak> eukaryotes 06:13 < drazak> that's actually the paper my teacher wrote 06:13 < gene> dang 06:13 < gene> then again 06:13 < gene> they also cut off the guy who had the idea of using GFP for signalling 06:18 < gene> so cells can exchange ATP? 06:18 < drazak> aye 06:18 < gene> interesting 06:18 < drazak> I'm going to ask him tuesday if he ever discovered why ATP is transgered so much better by connexin 43 than by connexin 32 06:19 < gene> reminds me of how modular robots distribute power among themselves 06:19 < drazak> heh 06:21 < gene> wonder if cells do it for the same reason 06:21 < drazak> no idea 06:22 < drazak> we don't even know what causes gap junction docking to happen 06:22 < drazak> whether or not there's a receptor or what 06:22 < gene> well then 06:22 < drazak> yeah 06:22 < gene> stress up some cells and watch them under a microscope 06:22 < drazak> well 06:22 < drazak> it's more than that 06:22 < drazak> but yeah 06:23 < drazak> there's like 23 types of connexins that have to do with gap junction dockings 06:23 < gene> get a cell to use up it's atp 06:23 < drazak> and certain ones are classified certain ways 06:23 < drazak> as in that it only talks to other cells with that class of connexin 06:24 < gene> interesting 06:24 < gene> that might be useful for say growing organs 06:24 < gene> do you know if kidney cells have connexins? 06:25 < drazak> probably 06:25 < drazak> I don't know a ton about it 06:25 < drazak> :P 06:25 < gene> kidney's require a lot of structure 06:25 < gene> might be easy to see if connexins help cells organise 06:26 < drazak> I have /no/ idea dude 06:26 < kanzure_> You're doing it wrong. 06:26 * kanzure_ goes away. 06:26 < gene> Am I? 06:26 < gene> how am I doing it wrong? 06:30 * drazak reads the full text 06:33 < gene> got a question for anyone out there 06:33 < gene> how do you purify proteins 06:34 < kanzure_> Antisomethings. 06:35 < gene> antibodies 06:35 < gene> damn 06:35 < gene> how do you make the antibodies? 06:36 < gene> inject the protein into a mouse? 06:37 < drazak> no 06:37 < drazak> that's not how 06:39 < kanzure_> Recombination + selection experiments. 06:39 < kanzure_> Kind of like aptamers IIRC. 06:39 < gene> ah 06:40 < gene> so is protein purification something that can be done by an amateur 07:38 < drazak> http://buffalo.craigslist.org/sys/831245536.html 07:39 < drazak> I just offered him $50-100 10:01 * willPow3r_ just installed 2nd life 10:01 < willPow3r_> hope its not as gay as wow 10:07 < hjkl> its useless 10:23 < willPow3r_> whys that 10:23 < willPow3r_> my guy has a gay ass hairdo and i can't seem to change it 12:13 < willPow3r_> 2nd life is soo so lame. 15:35 < bkero> Second life is so lame. 15:36 < hjkl> life is so lame. 15:36 < hjkl> ^^ 15:43 < ybit> lame 15:43 < bkero> UR so lame 15:43 < ybit> :D 15:43 < pppk> but dude, michio kaku loved teh second life 15:43 < pppk> can't be that bad 15:43 < bkero> OpenOffice 3 is going to be released tomorrow. 15:43 < bkero> It's on the FTPs already though. 15:49 * ybit needs to create a bot which mentions Lite Sm everytime Second Life is brought up 15:50 < bkero> Lite Sm? 15:51 < ybit> https://www.litesim.com 15:51 < ybit> not as bloated 15:51 < ybit> and it is foss 15:51 < bkero> lol 15:52 < ybit> you laugh because you, my friend, are lame ;) 15:52 < bkero> Vrey lame 15:52 < bkero> Of considerable lamitude 15:52 < ybit> hehe 15:58 < fenn> whatever happened to VRML 15:59 < fenn> hmm i wonder what is litesim's target market 16:01 < fenn> and why is it $24.99/mo? 16:02 < fenn> this is bad architecture: "By default all regions and grids are linked with the supergrid - a set of services which enable centralised management of all the grids and regions we host and centralised login." 16:40 < ybit> they don't allow direc connections to the lsmainisland due to security concerns, but you can setup your own grid and link it to the main grid to allow avatar migration 17:40 < fenn> matweb says nothing about engineered basalt :( 18:53 < fenn> here's a gem from the past: http://fennetic.net/pub/irc/replicator_notebook.jpg 18:53 < fenn> first attempt at supply chain minimization 19:41 < willPow3r> kinda 19:41 < willPow3r> wrong window sry 19:42 < willPow3r> x-mouse can lead to "wtf where the hell did my text go... oh, fuck..." moments 20:59 < fenn> remember to document blatantly obvious things in case one day you will be reading your lab notebook and be wondering wtf you were trying to do 21:00 < kanzure_> context? 21:00 < kanzure_> also, sleeping sucks 21:00 < fenn> me looking at my old research notebook, procrastinating resume writing etc 21:01 < fenn> also, how am i supposed to send a letter of recommendation electronically without looking at it? 21:01 < fenn> there's no such thing as a"digital sealed envelope" 21:02 < kanzure_> what if they just so happen to have their PGP key? 21:03 < fenn> "they" probably dont know what a PGP key is, and "the other they" doesnt have one 21:26 < kanzure> Hey, is that Deepak? 21:28 < ybit> http://www.osaerospace.com/wiki/tiki-index.php?page=Linkdumps -- neat 21:28 < ybit> anyone know of a spacecraft design far along? 21:28 < kanzure> Isn't that me? 21:28 < kanzure> There's no zip file to my knowledge that I can just hand you, no. 21:29 < fenn> what do you mean "spacecraft design"? 21:30 < ybit> something similar to http://www.asi.org/adb/04/02/spacecraft-info.html 21:30 < kanzure> Yay Artemis Society. 21:30 < kanzure> http://moo.asi.org/ has some giants in the industry in there. Just FYI. :) 21:31 < kanzure> Wife of Goddard or something :p 21:31 * fenn trembles in awe 21:32 < drazak> hey, the ligands interacting with signal receptors and ion channels on cells are not the organometallic ligands, right? 21:32 < fenn> "the organometallic ligands"? 21:33 < fenn> organometallic just means it has a metal ion stuck to it, but those are usually used for metabolism/catabolism 21:33 < drazak> right, but those are different ligands, right? 21:34 < drazak> but when you talk abotu organometallic interactions, you talk about ligands 21:34 < fenn> i suppose 21:34 < fenn> proteins can interact with metal ions too 21:35 < drazak> right 21:35 < drazak> but: 21:35 < fenn> um, here's an example: EDTA is an organometallic ligand if it picks up some metal ion 21:36 < drazak> "Cells also have surgace membrane proteins (signal receptors) that present highly specific binding sites for extracellular signall moleculse (receptor ligands). When an external ligand bonds to its specific receptor, the receptor protein transduces teh signal carried by the ligand into an intracellular message. 21:37 < fenn> oh, that just means the molecule that binds to the signal receptor 21:37 < drazak> right 21:37 < fenn> ligand = binder, in latin 21:37 < fenn> or something like that 21:37 < drazak> ah ok 21:37 < drazak> I don't know much latin 21:37 < fenn> me either 21:40 < fenn> can anyone here unstuff .sit files? 22:03 < kanzure> is it a compression format? 22:03 < kanzure> I have some illegal software in the lab that uncompresses nearly everything known to man 22:08 < fenn> yes it's some old macintosh format 22:08 < pppk> what's the software called bryan? 22:10 < kanzure> stuffit 22:14 < fenn> well it's here if anyone wants to give it a shot.. i'm not sure exactly what's in here: http://fennetic.net/pub/irc/Ben.sit 22:15 < fenn> i'm hoping its my old lab reports 22:39 < pppk> I can't stand the suspense 22:39 < pppk> what's in it? 22:43 < fenn> pppk: i would certainly like to find out! 22:43 < kanzure> so the algOS guy wants to hook up with me 22:44 < fenn> it's really amazing how little i remember from what i ostensibly learned in college 22:44 < fenn> i'm reading all these old emails and while i remember the people, i have no idea what i'm talking about 22:46 < kanzure> for instance? 22:46 < kanzure> random ranting? 22:46 < kanzure> "rawr you're doing yer integrals all backasswards" 22:46 < fenn> classification and structure of the ebola virus was the one i just read 22:47 < fenn> apparently i gave a presentation on it (whoda thunk) 22:47 < kanzure> hey, what's up with kanzure___ ? 22:48 < fenn> it's full moon so he has a tail :) 22:48 < fenn> just dont feed him after midnight or he'll turn into a giant ape 22:49 < kanzure> it's true, he used to howl at the moon 22:52 < fenn> "We found 45 kilos of flour the other day 22:52 < fenn> in a dumpster, and there was a pancake party. Then there was a tickling 22:52 < fenn> party, a pizza party, and a yerba matte party. We're way cooler than frat 22:52 < fenn> boys who have just beer parties." 23:53 -!- jk4930_ is now known as jk4930