--- Day changed Sun May 10 2009 01:34 -!- any10968429 is now known as katsmeow 01:36 -!- katsmeow is now known as katsmeow-afk 03:43 < xp_prg> are there any videos yet of this infared protein? 05:11 < drazak> fenn: can you name a few assays that use many reagent steps? 05:18 < drazak> you guys know if kanzure is gonna be back any time soon? 05:25 < genehacker> he might 05:26 < drazak> can you guys name a few biochemical assays that use multiple reagent steps in the same container? 05:35 < drazak> ok 05:35 < drazak> so how about this 05:35 < drazak> glass chip 05:35 < drazak> with 2 areas that contain fluid 05:37 < drazak> one of which has a way to add more to it via capilary action, or whatever, the outside one has one of those dna boxes containing two compounds, one that dissolves endothermically, one that disolves exothermically, in the inner fluid area that can have fluids added to it, there are many of those dna boxes present, each with different reagents in it, so that you can do a multitude of tests, depending on what kind of light it's exposed to 05:40 < drazak> the outer fluid area is to provide the proper temperature, such that if you're using something like benedicts reagent you could provide the heat easily 05:41 < drazak> it would be great for quick field tests 05:41 < genehacker> cool 05:41 < genehacker> read some books on microfluidics 05:42 < drazak> I've read up a little on it 05:42 < genehacker> you'll find them very interesting 05:42 < drazak> mhm 05:42 < drazak> I don't have enough time to learn as much as I'd like 05:42 < drazak> maybe this summer 05:42 * drazak is full time highschool student 05:43 < drazak> you might be able to make it cheaper by making compound dna boxes 05:43 < drazak> less surfaces 05:45 < drazak> you could also have different boxes full of different reagents in the external area, thus allowing cycles of heating and cooling 05:45 < genehacker> if the boxes are stable enough... 05:46 < drazak> yeah, I thought about that too 05:46 < drazak> I think dna takes a fairly high temperature to denature 05:46 < drazak> and if the boxes form spontaneously, they probably are fairly stable 05:47 < genehacker> how high is high? 05:47 < genehacker> boiling point? 05:49 < drazak> dunno 05:49 < drazak> not enough research done yet 05:50 < genehacker> well I want to make a dna synth over the summer 05:50 < genehacker> a dna synth capable of 62 mega base pairs 05:50 < drazak> you could build these dna boxes with that 05:50 < drazak> I think they're only like a few hundred kbp 05:51 < genehacker> heck I could build J craig venter 05:51 < genehacker> 's synthetic life form with it 05:51 < drazak> haha 05:51 < genehacker> he posted the sequence online 05:51 < drazak> is heybryan offline? 05:51 < genehacker> I need a cheap DLP projector to do it 05:52 < genehacker> I believe he's in meatspace 05:52 < drazak> fuck 05:52 < drazak> I needed to grab the nature paper from him 05:52 < drazak> it'll tell me exactly how many bp it is 05:52 < genehacker> damn 05:52 < genehacker> I don't know if we have it yet 05:52 < drazak> we do 05:53 < drazak> he mentioned it in his email 05:53 < drazak> he even linked it 05:53 < genehacker> damn 05:53 < drazak> yeah 05:53 < drazak> I know 05:53 < drazak> you know, there's another good thing about this 05:54 < drazak> you could make it so that the dissolved reagents are favorablely interacting with the dna box (design the box in such a way) so that they won't go bad (IE oxidize) 05:55 < drazak> need lots of computers to design that crap though 05:56 < genehacker> we have access to a supercomputer down here 05:57 < drazak> mhm 05:57 < genehacker> hmmm... would be synthesizing mycoplasma laboratorium be patent infringement 05:58 < genehacker> write the program and we'll run it 05:58 < drazak> you know what would be cool 05:58 < drazak> you could probably pcr these dna boxes 05:58 < drazak> to make more 05:59 < genehacker> yeah 06:00 < drazak> that would be pretty cool 06:02 < drazak> how do they get dna polymerase A? 06:03 < drazak> (aka the one used in pcr) 06:05 < genehacker> dunno 06:06 < genehacker> that's what we need to make 06:06 < drazak> yeah 06:06 < drazak> that seems to be the expensive part of this whole process, unless I'm missing something 06:07 < drazak> you only need to make each type of box once, or even once in each lab 07:29 < fenn> drazak: taq polymerase is typically synthesized by expression in recombinant e. coli 07:30 < fenn> to build the boxes you'd have to add a digestion step (restriction enzymes) after PCR 07:32 < fenn> if you can get your hands on the taq polymerase sequence, supposedly it's pretty easy to recover the enzyme.. purifying it is another matter though (and i'd try to build some purification into the sequence, like an easily cleave-able binding site) 07:43 < drazak> mhm 15:47 -!- fenn_ is now known as fenn 16:17 -!- any23948583 is now known as katsmeow-afk 18:06 -!- any14432598 is now known as katsmeow-afk 18:12 -!- any98226738 is now known as katsmeow-afk 18:18 -!- any34912125 is now known as katsmeow-afk 18:39 -!- any71122580 is now known as katsmeow-afk 19:52 < fenn> i love this image http://esamultimedia.esa.int/images/spacecraft-operations/space_debris/Bee-Hive-6_H1.jpg 20:36 < drazak> hah