--- Day changed Tue Jun 23 2009 00:10 < ybit> s/cathode ray/electron gun from a crt 00:11 < ybit> http://en.wikipedia.org/wiki/File:Scheme_TEM_en.svg 00:19 < fenn> there's a lot more to it than just the electron gun 00:19 < fenn> the TV choke coil is probably shaped all wrong for use in an SEM 00:19 < fenn> it's part of the flyback converter (HV power supply) so you'd need to make a new coil, and once you've done that you might as well just make everything from scratch 00:20 < ybit> http://itsyourexperiment08.wordpress.com/2008/10/24/diy-microscope-macgyver-does-leeuwenhoek/ 00:21 < fenn> interesting how the black and white scan is preserved in the history of the SVG file 00:24 < ybit> http://parkerlab.bio.uci.edu/microscopy_construction/build_your_own_twophoton_microscope.htm 00:26 < ybit> time for sleep 01:15 < fenn> damn chocolate 01:16 < fenn> i'd be asleep now if it werent for you! 01:43 < bkero> wootoff 02:49 < fenn> wow 'dot' really sucks at drawing hierarchies 07:24 < kanzure> ok. archived the twophoton microscope page, and the parkerlab documents. 07:24 < kanzure> http://adl.serveftp.org/papers/parkerlab-UCI/ 07:25 -!- ybit [n=ybit@unaffiliated/ybit] has quit ["Lost terminal"] 07:37 < kanzure> apt-get install hwb 08:17 < kanzure> http://reprapsource.com/ 09:50 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has joined #hplusroadmap 10:44 < kanzure> todo: wget http://www.iop.org.ezproxy.lib.utexas.edu/EJ/article/0960-1317/19/1/015007/jmm9_1_015007.pdf -o "A low temperature surface modification assisted method for bonding plastic substrates 10:44 < kanzure> " 10:44 < kanzure> er.. 10:46 < kanzure> ok, it's on adl.serveftp.org now --- Log closed Tue Jun 23 10:50:31 2009 --- Log opened Tue Jun 23 11:03:20 2009 11:03 -!- kanzure [n=bryan@cpe-72-177-123-170.austin.res.rr.com] has joined #hplusroadmap 11:03 -!- Irssi: #hplusroadmap: Total of 17 nicks [0 ops, 0 halfops, 0 voices, 17 normal] --- Log closed Tue Jun 23 11:10:48 2009 --- Log opened Tue Jun 23 11:31:45 2009 11:31 -!- kanzure [n=bryan@cpe-72-177-123-170.austin.res.rr.com] has joined #hplusroadmap 11:31 -!- Irssi: #hplusroadmap: Total of 17 nicks [0 ops, 0 halfops, 0 voices, 17 normal] 11:31 -!- Irssi: Join to #hplusroadmap was synced in 1 secs 11:40 < kanzure> bad programming joke of the day: http://imgur.com/nb9LO.jpg 12:00 -!- genehacker [n=chatzill@wireless-128-62-156-116.public.utexas.edu] has joined #hplusroadmap 12:07 < kanzure> genehacker: know of a differential equation that relates a gradient to an area, or something? 12:07 < genehacker> no why? 12:07 < kanzure> testing out skdb 12:07 < genehacker> how? 12:08 < kanzure> the submodule that deals with units in equations 12:08 < genehacker> oh cool 12:08 < genehacker> what are you trying to get it to do? 12:09 < kanzure> integrate with respect to a unit 12:09 < kanzure> (and other things) 12:09 < genehacker> can't some programs already do that? 12:09 < kanzure> like what? 12:12 < genehacker> wolfram alpha, my calculator 12:12 < kanzure> neither are open source 12:12 < kanzure> I'm using sympy, the symbolic python library for equations 12:12 < kanzure> http://sympy.org/ 12:12 < kanzure> going to be submitting a patch to them soon I guess 12:13 < kanzure> sympy.physics.units is somewhat of a joke :( 12:13 < kanzure> a bad joke, that is 12:13 < genehacker> http://arxiv.org/abs/physics/0703013 12:13 < genehacker> hey you don't think you could figure out how the toroidal form levitator in this paper works 12:13 < genehacker> if it works 12:14 < kanzure> not right now, I'm busy acting like I'm busy 12:14 < genehacker> ok 12:18 < genehacker> if the calculations are correct, it's possible to make a device for flying around in the air 12:18 < genehacker> and all it takes is some sort of superconducting toroid 12:18 < kanzure> like an airplane 12:18 < genehacker> like superman 12:19 < genehacker> though he doesn't explain how the simpler form of the device works 12:21 < genehacker> from the looks of it, it should only be capable of going east-west 12:22 < genehacker> but he says that it can go north south and east west 12:34 < ybit> this has become old, but can someone please upload a log of the channel for the past two days? i try to maintain a decent log file 12:36 < kanzure> um. actually, no. I have had connection problems. 12:37 -!- kanzure- [i=bryan@dhcp-84-36.me.utexas.edu] has joined #hplusroadmap 12:39 < ybit> fenn told me the connection was 'fubared' yesterday, so i understand. but maybe fenn, genehacker, or any idlers could? 12:39 < genehacker> ummmmm.... I don't have internet where I live 12:39 < ybit> bkero, katsmeow-afk, and nsh are always in here 12:39 < kanzure> ybit: fenn is on the same connection. 12:40 < bkero> I'm always here. 8) 12:40 < ybit> a little help then? :) 12:40 < bkero> ybit: I'll upload my log for the channel for the last long while 12:41 < ybit> ty 12:41 < bkero> ybit: http://staff.osuosl.org/~bkero/#hplusroadmap.gz 12:44 < genehacker> hmmm.... flexible superconducting tape 12:45 < kanzure> I think you meant .log.gz, bkero 12:50 < ybit> bkero: i was looking for logs from the past few days, i.e. this past weekend and yesterday :P 12:51 < bkero> kanzure: You're right 12:51 < bkero> ybit: Sorry, it's either you that has to tail -2000 it, or me 12:55 < ybit> i'll do it 12:58 < ybit> just need the log of last three days to do so though 12:58 < kanzure> import sympy.physics.units as units 12:58 < kanzure> import sympy 12:59 < kanzure> type(units.m + units.m) == 12:59 < kanzure> wtf? 12:59 < ybit> #sympy ?? 12:59 < kanzure> yeah but I still get to complain to you 12:59 < ybit> :P 12:59 < fenn> here's what i was working on last night.. it could probably use some graphviz tweaking: http://adl.serveftp.org/lab/fenn/taxonomy-neato.png 13:00 < fenn> taxonomy of manufacturing processes 13:00 < kanzure> so.. hierarchies? 13:01 < ybit> fenn: do you have this in a svg? a lot easier to grep 13:01 < fenn> better than that 13:02 < genehacker> wow 13:02 < genehacker> what about robotcasting? 13:04 < genehacker> err robocasting 13:04 < fenn> perhaps kanzure would like to get gitweb working so i dont have to do silly git commands on the server 13:10 -!- tty1 [n=freemo@unaffiliated/electric-penguin/x-9957366] has joined #hplusroadmap 13:13 < fenn> ybit: sorry i'm so slow http://adl.serveftp.org/skdb/taxonomy-graph.py http://adl.serveftp.org/skdb/taxonomy.yaml 13:17 -!- anthonyl [n=duzt@dsl093-216-054.aus1.dsl.speakeasy.net] has quit [Read error: 60 (Operation timed out)] 13:22 < genehacker> hmmm... 13:31 < ybit> i'm converting it to a svg 13:31 < ybit> might take a day or two 13:33 < ybit> oh nm 13:33 < ybit> .yaml is just fine 13:37 < fenn> dot does .svg output btw 13:40 < fenn> http://adl.serveftp.org/lab/fenn/taxonomy-neato.svg 13:47 < ybit> that's helpful 13:51 < genehacker> could you spread it out so you can read everything? 13:51 < fenn> i was trying to make something more like a cladogram though 13:54 < genehacker> I think I've seen one of these before 13:54 < kanzure> I have found it odd how almost every modern mother has a box of band-aids in the medicine cabinet, but I still don't have a dusty DNA synthesizer in my attic. 13:55 < ybit> bkero: is it inconvenient for you to post a log of the last two days? 13:57 < genehacker> well think about it kanzure 13:57 < fenn> like this http://www.palaeos.com/Invertebrates/Molluscs/Bivalvia/cladogram.html or http://www.gavinrymill.com/dinosaurs/Cladogram/CladogramComplete.jpg 13:57 < genehacker> why would you want a DNA synthesizer? 13:57 < genehacker> you can just buy synthesized DNA from companies 13:57 < genehacker> there's no need 13:57 < genehacker> or that's what the companies that sell synthesized DNA say 13:58 < kanzure> so what if that's what they say? 13:58 < kanzure> "why would you need a brain when you can just buy one" 13:58 < kanzure> "why would you need a brain when you can just buy thinktime from Google?" 13:59 < genehacker> yeah 13:59 < fenn> i wish i could buy thinktime from google 13:59 < fenn> but the bastards give it away for free! 13:59 < genehacker> synthetic DNA chemicals are fairly hard to come by 14:00 < kanzure> what is this analysis based off of, genehacker? 14:00 < kanzure> have you figured out how to synthesize GNA yet? 14:00 < genehacker> no 14:00 < genehacker> hmmm... 14:00 < genehacker> maybe not 14:01 < kanzure> fenn: if you bought thinktime from google, then you would have to use that thinktime in order to get some return on investment otherwise you'll run out of thinktimes on google 14:01 < genehacker> I might have found a website that sells cheap( ~$25) photolabile? phosphoramidite 14:01 < genehacker> s 14:02 < genehacker> the thing about synthesizing GNA is that we have to make photolabile GNA phosphoramidites 14:04 < bkero> ybit: It would be just as inconvenient for me as it is you 14:04 < genehacker> I don't think anyone's done that yet 14:04 < kanzure> why not make the photolabile phosphoramidites? 14:04 < ybit> bkero: why is that? 14:05 < ybit> i don't have the log file 14:05 < fenn> didnt he just post the link? 14:05 < ybit> yeah, but it ends on friday 14:05 < ybit> i was looking for one of the past 2 days 14:05 < genehacker> why? 14:06 < genehacker> I don't know how 14:06 < bkero> ybit: What would you do to cut it down to 2 days? 14:06 < ybit> you could tail or manually copy and paste to a pastebin 14:07 < ybit> or copy/paste to a .txt file on the server 14:07 < bkero> Any reason you couldn't do that? I'm actually in class 14:07 < ybit> because i don't have the log file 14:07 < fenn> seems the log ends on may 15 14:07 < fenn> the one bkero linked to 14:07 < ybit> right 14:08 < bkero> Damn irc logging, guess I don't have it then 14:08 < ybit> bkero: alright, and i don't need to bother you right now anyway 14:08 < ybit> fenn, you were on for most of the time i was... 14:09 < genehacker> but GNA synthesis is pretty simple 14:09 < fenn> fine fine 14:09 < ybit> :) 14:09 < fenn> it's not like you missed anything 14:09 < genehacker> it's only 3 steps 14:10 < kanzure> genehacker: ammonia kinase 14:11 < genehacker> ??? 14:11 < genehacker> what's that? 14:11 < fenn> ybit: http://adl.serveftp.org/last-two-days.log 14:11 < genehacker> interesting 14:12 < kanzure> http://en.wikipedia.org/wiki/Ammonia_kinase 14:12 < ybit> ty fenn 14:12 < kanzure> "Thus, the two substrates of this enzyme are ATP and NH3, whereas its two products are ADP and phosphoramide." 14:12 < genehacker> ok now how do we make photolabile phosphoramidites from that? 14:14 < ybit> fenn: what irc client do you use? that would be great to have a client or plugin which presents the conversation from all rooms like that 14:14 < fenn> irssi 14:15 < fenn> i actually wasnt trying to do that, i just messed up the logging settings somehow 14:15 < bkero> ybit: http://staff.osuosl.org/~bkero/hplus.log.gz 14:24 < ybit> thanks bkero, was retrieved 14:29 < kanzure> phosphoramidate = H2NO3P-2 14:29 < kanzure> phosphoramide = H6N3OP 14:31 < kanzure> genehacker: this is a basic chemistry problem, btw. aminos and estrifications are quite common. 14:31 < kanzure> for instance, 14:31 < kanzure> an ester of H3PO3 would be formed by condensation of an alcohol and an acid, plus subsequent elimination of water 14:31 < genehacker> oh cool 14:31 < genehacker> guess I need to learn ochem 14:31 < genehacker> got any books on it? 14:31 < kanzure> stacks of them 14:31 < kanzure> that ester (er, the phosphite) is useful in making phosphoramidite 14:32 < kanzure> which isn't the same thing as making a phosphoramide 14:32 < genehacker> virtual books 14:32 < kanzure> yes 14:33 < genehacker> link 14:33 < kanzure> server is currently inaccessible to pesky outsiders, sorry 14:34 < kanzure> maybe I'll copy some files to a thumb drive for you and get them on the lab server by tomorrow 14:34 < fenn> you should get rauchwerk to toss you some fluffy clouds 14:34 < kanzure> he's too busy trying to find actual data 15:05 < kanzure> pentaerythritol synthesis setup: http://www.orgsyn.org/orgsyn/orgsyn/prepContent.asp?prep=CV1P0425 15:07 < kanzure> http://asmeurersympy.wordpress.com/2009/06/22/how-to-permanently-lose-data-with-git-and-then-retrieve-it-again/ 15:07 < kanzure> (colored prompt?) 15:10 < kanzure> genehacker: I am less interested in how to synthesize phosphoramidites as I am interested in figuring out how in the hell you plan to do protecting groups 15:22 -!- genehacker [n=chatzill@wireless-128-62-156-116.public.utexas.edu] has quit [Read error: 110 (Connection timed out)] 15:42 < kanzure> NPPOC chloride sounds like the easiest to make. 16:17 -!- digitaltao [n=digitalt@cpe-72-179-5-147.austin.res.rr.com] has quit [Read error: 60 (Operation timed out)] 16:30 -!- tty1 [n=freemo@unaffiliated/electric-penguin/x-9957366] has left #hplusroadmap [] 16:31 < fenn> ok the latest rendering looks a lot better: http://adl.serveftp.org/lab/fenn/taxonomy-small.png 16:32 < kanzure> 1 g of NPPOC-dC(ib) Amidite from Sigma-Aldrich: $480 16:32 -!- digitaltao [n=digitalt@cpe-72-179-5-147.austin.res.rr.com] has joined #hplusroadmap 16:32 < fenn> and here's one for you ybit: http://adl.serveftp.org/lab/fenn/taxonomy.svg 16:32 < fenn> but the coloring's different since it's random 16:33 < fenn> i suppose i could hash the node name and use that as the random seed or something 16:33 < kanzure> or just save the seed 16:34 < kanzure> (er, that you used in the first place) 16:38 -!- genehacker [n=chatzill@wireless-128-62-156-116.public.utexas.edu] has joined #hplusroadmap 16:41 -!- digitaltao_ [n=digitalt@cpe-72-179-5-147.austin.res.rr.com] has joined #hplusroadmap 16:44 < kanzure> genehacker: do you know how to synthesize photolabile protecting groups? 16:54 -!- digitaltao [n=digitalt@cpe-72-179-5-147.austin.res.rr.com] has quit [Read error: 110 (Connection timed out)] 17:01 -!- genehacker [n=chatzill@wireless-128-62-156-116.public.utexas.edu] has quit [Read error: 110 (Connection timed out)] 17:15 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has quit [Read error: 110 (Connection timed out)] 17:19 -!- PeerInfinity [n=someone@216.36.180.162] has joined #hplusroadmap 18:04 < kanzure> wonder why genehacker got all uppity about having to make nucleobases 18:04 < kanzure> nucleotidases do it for you .. 18:04 < kanzure> http://en.wikipedia.org/wiki/Nucleotidase 18:06 < kanzure> er, besides the sorting problem 18:06 < kanzure> which I guess could be solved by gel electrophoresis and isolating different strands of DNA with mainly one of the four bases each 18:33 -!- digitaltao_ [n=digitalt@cpe-72-179-5-147.austin.res.rr.com] has quit [Read error: 60 (Operation timed out)] 18:34 -!- digitaltao_ [n=digitalt@cpe-72-179-5-147.austin.res.rr.com] has joined #hplusroadmap 19:01 < kanzure> ybit: did you take my keys? 20:07 < kanzure> blah = pq.Quantity([1],'J*s') 20:07 < kanzure> blah.simplified --> kg*m**2/s 20:08 < kanzure> [1] s*J 20:08 < kanzure> print q.dimensionality -> s*J 20:09 < kanzure> print q.simplified -> [ 1.] kg·m²/s 20:10 < kanzure> hm. 'J*s^2' is apparently the same as 'J*s**2' 20:24 < kanzure> inspect.getargspec() looks useful 20:25 < kanzure> but I'm not sure how much this is going to help to figure out which methods of a package compute various units, like Screw.breaking_force() for instance 20:31 < fenn> one way is to make a conversion table/tree with references to the functions 20:31 < fenn> the other way is to put some string(?) "look at me i convert torque to force" in the functions 20:31 < fenn> but breaking_force() isn't even a conversion really (is it?) 20:38 -!- genehacker [n=chatzill@wireless-128-62-124-98.public.utexas.edu] has joined #hplusroadmap 20:49 < kanzure> genehacker: you do not add anything to protecting groups. 20:49 < kanzure> protecting groups exist so that when you shine light at them, they cleave and everything is unprotected 20:49 < kanzure> then the chemical reactions are able to proceed 20:49 < kanzure> thus, synthesis. 20:50 < genehacker> not even nitrogenous bases? 20:50 < kanzure> what? 20:50 < kanzure> what are you asking? 20:51 < genehacker> the photolabile phosphoramidites I read about have protecting groups that cleave off in the presence of light so the next nucleotide can be add 20:51 < genehacker> you expose where you want the A's to go 20:52 < genehacker> put down the A's and they stick 20:52 < kanzure> what does that have to do with nitrogenous bases 20:52 < kanzure> or, rather, what does that have to do with your question 20:52 < genehacker> we need NPPOC -deoxyadenosine and what not 20:53 < genehacker> how do we get the deoxyadenosine? 20:53 < kanzure> NPOCC-Cl means NPOCC chloride 20:53 < genehacker> yeah 20:53 < kanzure> not deoxyadenosine 20:53 < genehacker> I know 20:53 < kanzure> then why did you say NPOCC-deoxyadenosine 20:54 < kanzure> anyway, here are some notes 20:54 < kanzure> http://adl.serveftp.org/papers/phosphoramidites/notes.txt 20:54 < genehacker> the next step is to make the stuff you remove the protecting group from during DNA synthesis 20:54 < kanzure> ok. 20:54 < genehacker> understand now? 20:54 < kanzure> no 20:57 < genehacker> you can't do dna synthesis with protecting groups alone correct? 20:57 < kanzure> that's correct 20:58 < kanzure> although someone did DNA synthesis on this four-branched dendrimer via condensation of acetaldehyde and formaldehyde 20:58 < kanzure> but it was a branched oligonucleotide in the end, not a double helix or single strand 20:59 < genehacker> so how do we use these protecting groups? 21:00 < genehacker> in your notes it says they can be used for peptide synthesis? 21:00 < drazak_> genehacker: think of a protecting group like this, it's like the paper on doublesided tape, you have to take it off before they can stick together 21:00 < genehacker> yeah I know that 21:00 < kanzure> you add them to the byproducts of the nucleotidases 21:00 < genehacker> what's the paper and what's the tape? 21:00 < kanzure> what? 21:00 < kanzure> there is no paper 21:01 < kanzure> you use nucleotidases to convert your nucleotides to nucleosides 21:01 < kanzure> you add your protecting groups to the nucleosides 21:01 < genehacker> ok 21:01 < kanzure> you shine light at your nucleosides+protecting-groups to unprotect it 21:01 < kanzure> you separate your nucleotides by gel electrophoresis 21:02 < kanzure> (er, that step was out of order) 21:02 < kanzure> um, and that "-" in "nucleosides+protecting-groups" should be removed (except that it's harder to read) 21:02 < genehacker> that's it? 21:03 < genehacker> I thought they modified the nucleosides a bit more than just protecting groups 21:03 < kanzure> where? 21:04 < genehacker> removing the protecting group turns the nucleoside into a nucleotide? 21:05 < kanzure> a nucleoside is a nucleotide without a phosphate. 21:05 < genehacker> yeah I know 21:05 < kanzure> removing the protecting group means that the nucleoside is able to react with the strand that you are synthesizing 21:05 < genehacker> how? 21:06 < kanzure> the protecting group isn't there to .. er, protect it 21:07 < kanzure> there's also some other steps in between each cycle of addition of course 21:07 < kanzure> otherwise ddNTPs would just combine into long chains when in storage in their little bottles :p 21:07 -!- genehacker [n=chatzill@wireless-128-62-124-98.public.utexas.edu] has quit [Remote closed the connection] 21:07 < kanzure> I think I killed him. 21:13 -!- genehacker [n=chatzill@wireless-128-62-124-98.public.utexas.edu] has joined #hplusroadmap 21:14 < genehacker> ugh why am I still using firefox for browsing? 21:14 < genehacker> so here's the deal photolabile phosphoramidites don't have steps in between 21:15 < kanzure> according to who? 21:15 < genehacker> according to the people who did maskless array gene synthesis 21:16 < kanzure> that's not helpful because I don't know the name of those people off the top of my head 21:17 < kanzure> apparently I am now admining abundancestudies.org 21:17 < kanzure> bah, no ssh access 21:20 < genehacker> I don't know who 21:22 < genehacker> so how do we get NPPOC-d-adenosine [N6-tac] β-cyanoethylphosphoramidite 21:22 < genehacker> http://genome.cshlp.org/content/12/11/1749.full 21:22 < genehacker> here 21:24 < genehacker> so we can make NPPOC-Cl 21:24 < genehacker> how do we get from that to d-adenosine [N6-tac] β-cyanoethylphosphoramidite 21:27 < genehacker> so I see 21:27 < kanzure> already told you how to make it 21:27 < kanzure> in the email 21:27 < kanzure> also in the link to the notes 21:28 < genehacker> just add a protecting group to a nucleoside? 21:28 < kanzure> http://adl.serveftp.org/papers/phosphoramidites/notes.txt 21:28 < ybit> what is abundancestudies.org? 21:28 < genehacker> well we need to try this out 21:28 < kanzure> no, NPPOC-Cl is a bit more complicated 21:28 < kanzure> ybit: it's joseph jackson's website 21:28 < kanzure> joseph is trying to make a Journal of Abundance 21:28 < ybit> about? 21:28 < ybit> oh 21:28 < kanzure> "Abundance" 21:29 < ybit> yeah, gl with that 21:29 < fenn> here is your git snack for the day: 21:29 < kanzure> sarcasm? 21:29 < fenn> git log --pretty=oneline --abbrev-commit 21:29 < ybit> kind of 21:29 < kanzure> now we need a catchy name for that 21:29 < fenn> alias gl='... 21:29 < kanzure> heh 21:30 < fenn> gl~~!! 21:30 < genehacker> what's the structure of NPPOC-Cl? 21:30 < ybit> sarcasm due to frustration of not recieving the workbench tonight 21:31 < kanzure> genehacker: I think you should read the notes. 21:31 < kanzure> NPPOC is basically 2-(2-Nitrophenyl) propoxycarbonyl 21:31 < fenn> sarcasm is just one more service i offer. chaos, panic, and disorder... my work here is done. 21:32 < ybit> and because "abundance" is highly general at this moment, not saying the journal won't have any substance, it's just hard to say much about something when there isn't a clear definition of what he's after 21:32 < genehacker> ok I think I get it now 21:32 < ybit> afk for a few 21:32 < genehacker> so we need to make some of it 21:32 < genehacker> why did you say it is hard? 21:32 < kanzure> who said that? 21:33 < genehacker> making NPPOC-Cl? 21:33 < kanzure> I don't think I did. 21:33 < kanzure> ybit: joseph actually has more thoughts about abundance than he actually bothers writing about. over the phone he's much more thorough. I heard him live on some blog-by-phone thingy. 21:34 < genehacker> no, NPPOC-Cl is a bit more complicated 21:34 < kanzure> what? 21:34 < kanzure> more complicated than what? 21:34 < genehacker> you said that 21:35 < kanzure> oh 21:35 < kanzure> more complicated than adding it to a nucleotide 21:35 < kanzure> yes, that's what I said. 21:35 < kanzure> there's a few apparati that you're going to have to build 21:35 < kanzure> like a distillation column 21:36 < genehacker> and I'm sure we need reagent grade stuff 21:36 < kanzure> fenn: are you just reading off of the coasters over there? 21:36 < kanzure> genehacker: not really 21:36 < kanzure> genehacker: the reaction yields are fairly high for the majority of these reactions 21:36 < genehacker> hmmmm 21:37 < genehacker> reagent grade means that what you have is very pure 21:37 < fenn> yield and purity are different things.. 21:37 < fenn> who is 'newell' named after? 21:37 < genehacker> exactly fenn 21:37 < kanzure> he should still look over the method before giving up 21:38 < fenn> i agree high purity reagents are not necessary for starters 21:38 < genehacker> good point 21:40 < genehacker> so how do we 1. get adequate quantities of DNA to digest 2. seperate out different nucleosides 21:40 < kanzure> are you seriously asking #1 ? 21:41 < kanzure> #2 would be some sort of super gel 21:42 < genehacker> not so sure a gel would seperate out different nucleotides 21:42 < kanzure> "A procedure for the rapid estimation of sulfated nucleotides has been developed in this laboratory using polyacrylamide gel electrophoresis for separation ..." 21:43 < kanzure> this doesn't sound like a super big problem 21:43 < genehacker> oh we can do TLC 21:43 < genehacker> and we can make our own TLC plates according to instructables 21:43 < genehacker> well 21:43 < genehacker> now this is starting to get interesting 21:43 < fenn> i'm really wary of any plan relying on 'according to instructables' 21:43 < kanzure> TCL? 21:43 < fenn> TLC is basically a glass microscope slide with a thin layer of alumina sintered on it 21:44 < fenn> it's like paper chromatography but more uniform and more inert 21:44 < genehacker> ok 21:44 < fenn> and not terribly useful for bulk quantities 21:44 < kanzure> "paper electrophoresis" huh 21:44 < fenn> also i'm not convinced you'd have enough separation between such similar molecules as the different bases 21:44 < genehacker> I don't think we need bulk quantities 21:45 < fenn> it's not electro- anything, the solvent moves along by capillary action 21:45 < genehacker> instructables has an article about making TLC plates from glass slides and silica gel packets 21:45 < fenn> genehacker: why are you considering making your own TLC plates? 21:46 < fenn> they also had an article about making a clean room by removing the carpet 21:46 < genehacker> why not? 21:46 < fenn> "if you're really serious" 21:46 < fenn> i think you do need bulk quantities 21:47 < kanzure> how much is bulk 21:47 < fenn> because you have to wash the plate off between every base synthesized 21:48 < genehacker> cool only one chemical is on the DEA's list II 21:48 < fenn> genehacker: kanzure says you claim you dont have to wash off the plate, but how could that possibly work? 21:48 < genehacker> and that's the one we can make if we modify some bacteria to make it 21:48 * fenn regrets asking already 21:49 < genehacker> I don't know, I have to go change my router's mac address so I can hopefully use the internet at my place 21:49 < fenn> genehacker: you owe me a cookie 21:50 < genehacker> sure you can have one 21:51 < genehacker> hmmm... I also have to figure out the password for it if the previous owner changed it 21:53 < fenn> push the button 21:54 < genehacker> that's all? 21:58 < kanzure> push the reset button 21:59 < genehacker> ok 22:01 < kanzure> fenn: did you ever bother to look over the data in these files? 22:01 < kanzure> http://adl.serveftp.org/papers/adesign/adesign-libraryEM.zip 22:01 < kanzure> (might be a tarbomb) 22:01 < kanzure> http://adl.serveftp.org/papers/adesign/adesign-edited.zip 22:01 < kanzure> ^ that one is definitely not a tarbomb, although larger 22:02 < genehacker> kanzure I'd recommend posting that stuff to diybio 22:02 < kanzure> what stuff 22:02 < fenn> look over the data? you mean the 3 or 4 columns of unlabeled numbers? (with two or three entries each) 22:03 < kanzure> yes 22:03 < genehacker> the stuff about making protecting groups and nucleosides 22:03 < fenn> yeah i looked at it 22:09 < fenn> irssi nickcolor.pl title.pl 22:09 -!- kanzure changed the topic of #hplusroadmap to: there is a disturbance in the cookie matrix 22:11 < fenn> in irssi-scripts 22:11 < fenn> move to .irssi/scripts/autorun/ 22:23 -!- genehacker [n=chatzill@wireless-128-62-124-98.public.utexas.edu] has quit [Read error: 110 (Connection timed out)] 22:37 -!- Netsplit niven.freenode.net <-> irc.freenode.net quits: tarbo2, kanzure-, bkero, xp_prg 22:37 -!- Netsplit over, joins: xp_prg 22:37 -!- Netsplit over, joins: kanzure-, tarbo2, bkero 23:21 < ybit> kanzure: what extropy mailing list did the "world’s first brain prosthesis" come from? 23:58 -!- PeerInfinity [n=someone@216.36.180.162] has quit [Read error: 110 (Connection timed out)]