--- Log opened Sat Apr 30 00:00:07 2011 00:00 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has joined ##hplusroadmap 00:02 -!- nchaimov [~nchaimov@c-71-59-157-3.hsd1.or.comcast.net] has quit [Quit: nchaimov] 00:08 -!- nchaimov [~nchaimov@c-71-59-157-3.hsd1.or.comcast.net] has joined ##hplusroadmap 00:28 -!- nchaimov [~nchaimov@c-71-59-157-3.hsd1.or.comcast.net] has quit [Quit: nchaimov] 00:29 -!- nchaimov [~nchaimov@c-71-59-157-3.hsd1.or.comcast.net] has joined ##hplusroadmap 00:34 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has quit [Quit: Leaving] 00:34 -!- uniqanomaly [~ua@dynamic-78-8-82-191.ssp.dialog.net.pl] has joined ##hplusroadmap 00:52 -!- klafka1 [~textual@cpe-66-66-10-44.rochester.res.rr.com] has joined ##hplusroadmap 01:19 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 01:59 -!- jenniside [jen@173-29-174-114.client.mchsi.com] has joined ##hplusroadmap 02:03 -!- jennicide [jen@173-29-174-114.client.mchsi.com] has quit [Ping timeout: 240 seconds] 02:52 -!- elmom [~elmom@hoasnet-fe13dd00-52.dhcp.inet.fi] has joined ##hplusroadmap 03:18 -!- augur [~augur@208.58.6.161] has quit [Read error: Connection reset by peer] 03:18 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 03:58 -!- JayDugger [~duggerj@pool-173-74-79-43.dllstx.fios.verizon.net] has quit [Quit: Leaving.] 05:53 -!- augur [~augur@208.58.6.161] has quit [Remote host closed the connection] 05:55 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 05:55 -!- augur [~augur@208.58.6.161] has quit [Remote host closed the connection] 05:55 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 05:56 -!- augur [~augur@208.58.6.161] has quit [Remote host closed the connection] 07:03 -!- JayDugger [~duggerj@pool-173-74-79-43.dllstx.fios.verizon.net] has joined ##hplusroadmap 07:12 -!- klafka1 [~textual@cpe-66-66-10-44.rochester.res.rr.com] has quit [Quit: Computer has gone to sleep.] 07:19 -!- klafka1 [~textual@cpe-66-66-10-44.rochester.res.rr.com] has joined ##hplusroadmap 07:27 -!- JayDugger [~duggerj@pool-173-74-79-43.dllstx.fios.verizon.net] has quit [Quit: Leaving.] 07:39 -!- klafka1 [~textual@cpe-66-66-10-44.rochester.res.rr.com] has quit [Quit: Computer has gone to sleep.] 08:13 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 09:23 -!- augur [~augur@208.58.6.161] has quit [Remote host closed the connection] 09:24 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 09:24 -!- augur [~augur@208.58.6.161] has quit [Remote host closed the connection] 09:25 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 09:45 -!- mayko [~mayko@71-22-217-151.gar.clearwire-wmx.net] has quit [Ping timeout: 240 seconds] 09:59 -!- mayko [~mayko@71-22-217-151.gar.clearwire-wmx.net] has joined ##hplusroadmap 11:24 -!- alystair [Alystair@bas1-toronto10-1279556049.dsl.bell.ca] has quit [Quit: ┌(・_・)┘OUTTA HERE└(・o・)┐] 11:42 -!- mjr [mjr@2001:1bc8:102:60d4:21b:fcff:fe32:5eda] has quit [Read error: Operation timed out] 11:44 -!- LukasDimoveo [6c1535f9@gateway/web/freenode/ip.108.21.53.249] has joined ##hplusroadmap 11:45 < kanzure> why do you keep changing names on us 11:45 < LukasDimoveo> because for some reason "Lukas" changes to Guest450645 11:46 < LukasDimoveo> or something like that 11:46 < kanzure> because it's registered 11:46 < kanzure> you should register a nickname too 11:47 < LukasDimoveo> how do I do this? 11:47 < kanzure> /query NickServ register 11:47 < kanzure> but make sure you set your nickname to what you really want, first 11:47 < LukasDimoveo> alright 11:47 < LukasDimoveo> Anyway 11:48 < LukasDimoveo> I want to do a simple bacterial transformation with bacteria commonly found in yogurt 11:48 < kanzure> which bacteria in particular? 11:49 < kanzure> lactobacillus xyz? 11:49 < LukasDimoveo> yup 11:50 < kanzure> once you know the actual name you can check for protocols on protocol-online.net 11:50 < cluckj> sounds like a delicious experiment 11:50 < LukasDimoveo> thank you 11:52 < LukasDimoveo> I am targeting the "low hanging fruit" of human genetic engineering - engineering gut flora is the way to go until I can get some more gene therapy experience 11:52 < kanzure> have you ever done a bacteria transformation project beforE? 11:52 < kanzure> i'd suggest starting with the typical ecoli project.. 11:53 < LukasDimoveo> I have done e.coli so many times I am sick of it 11:53 < kanzure> it's a weekend project if anything 11:53 < kanzure> ok 11:53 < LukasDimoveo> (thinks about the 9-5 summer job - shutters) 11:54 < LukasDimoveo> anyway, thanks for the link 11:54 < kanzure> blah i should go pick out a dna sequencer for that guy today 11:55 < LukasDimoveo> which guy, where? 11:59 < kanzure> local guy 12:00 < kanzure> i'm trying to figure out which model i want to buy 12:00 < kanzure> so i thought i'd get the same model (abi prism 310) as the socal-diybio group 12:00 < kanzure> but nothing is showing up on ebay at the moment, plus they have had.. problems with it 12:08 < LukasDimoveo> Good to know 12:08 -!- mjr [mjr@2001:1bc8:102:60d4:21b:fcff:fe32:5eda] has joined ##hplusroadmap 12:33 < cluckj> kanzure do you still have that transcript of the FBI dude at the open science summit? 12:44 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has joined ##hplusroadmap 12:58 < kanzure> cluckj: http://diyhpl.us/wiki/transcripts/open-science-summit-2010/ed-you/ 13:02 -!- LukasDimoveo [6c1535f9@gateway/web/freenode/ip.108.21.53.249] has quit [Quit: Page closed] 13:04 < kanzure> hm that's weird 13:04 < kanzure> jrayhawk: if you go to http://diyhpl.us/wiki/recentchanges/ 13:04 < kanzure> and then to the 2010-07-24 edits to 'cadfaq', 13:04 < kanzure> it sends you to https://secure.diyhpl.us/repos/diyhpluswiki/ikiwiki.cgi?page=cadfaq&do=goto 13:04 < kanzure> which requires a login 13:06 < kanzure> er, well, the 'diff' is on /cgit so it's accessible 13:06 < kanzure> that link is just a poorly formed redirect 13:10 < cluckj> kanzure thanks 13:10 < cluckj> I'm writing a paper about it 13:12 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has quit [Remote host closed the connection] 13:34 < kanzure> cluckj: i've added some stuff to http://diyhpl.us/wiki/transcripts/ 13:43 < cluckj> nice 13:43 < cluckj> are you going to the diy bio continental congress? 13:46 < kanzure> no 13:47 < kanzure> i'm taking a break from conferences :p 14:44 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has joined ##hplusroadmap 15:25 -!- gnusha [~gnusha@131.252.130.248] has quit [Ping timeout: 246 seconds] 15:26 -!- gnusha [~gnusha@131.252.130.248] has joined ##hplusroadmap 15:26 -!- Topic for ##hplusroadmap: http://gnusha.org/skdb/ http://groups.google.com/group/diybio http://bit.ly/diybionews http://gitduino.com/ http://gadaprize.org/ | logs: http://gnusha.org/logs/ 15:26 -!- Topic set by kanzure [~kanzure@131.252.130.248] [Thu Jan 20 10:44:20 2011] 15:26 [Users ##hplusroadmap] 15:26 [ AJollyLife] [ Drakkar ] [ Helleshin] [ kanzure__] [ saurik ] 15:26 [ AlonzoTG1 ] [ drazak ] [ Jappe2 ] [ mayko ] [ streety ] 15:26 [ augur ] [ elmom ] [ jebba ] [ mjr ] [ Tyrant ] 15:26 [ bkero ] [ epitron ] [ jenniside] [ nchaimov ] [ uniqanomaly] 15:26 [ CIA-92 ] [ eridu ] [ jmil ] [ nsh ] [ Utopiah ] 15:26 [ cluckj ] [ fenn ] [ jrabbit ] [ panax ] [ ybit ] 15:26 [ dbolser ] [ ferrouswheel] [ jrayhawk ] [ pasky ] 15:26 [ devrandom ] [ gnusha ] [ kanzure ] [ QuantumG ] 15:26 -!- Irssi: ##hplusroadmap: Total of 38 nicks [0 ops, 0 halfops, 0 voices, 38 normal] 15:26 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 15:26 -!- Irssi: Join to ##hplusroadmap was synced in 4 secs 16:03 -!- CryptoQuick [~CryptoQui@c-174-51-232-237.hsd1.co.comcast.net] has joined ##hplusroadmap 16:42 -!- gnusha [~gnusha@131.252.130.248] has quit [Ping timeout: 246 seconds] 16:42 -!- gnusha [~gnusha@131.252.130.248] has joined ##hplusroadmap 16:42 -!- Topic for ##hplusroadmap: http://gnusha.org/skdb/ http://groups.google.com/group/diybio http://bit.ly/diybionews http://gitduino.com/ http://gadaprize.org/ | logs: http://gnusha.org/logs/ 16:42 -!- Topic set by kanzure [~kanzure@131.252.130.248] [Thu Jan 20 10:44:20 2011] 16:42 [Users ##hplusroadmap] 16:42 [ AJollyLife ] [ devrandom ] [ gnusha ] [ kanzure ] [ QuantumG ] 16:42 [ AlonzoTG1 ] [ Drakkar ] [ Helleshin] [ kanzure__] [ saurik ] 16:42 [ augur ] [ drazak ] [ Jappe2 ] [ mayko ] [ streety ] 16:42 [ bkero ] [ elmom ] [ jebba ] [ mjr ] [ Tyrant ] 16:42 [ CIA-92 ] [ epitron ] [ jenniside] [ nchaimov ] [ uniqanomaly] 16:42 [ cluckj ] [ eridu ] [ jmil ] [ nsh ] [ Utopiah ] 16:42 [ CryptoQuick] [ fenn ] [ jrabbit ] [ panax ] [ ybit ] 16:42 [ dbolser ] [ ferrouswheel] [ jrayhawk ] [ pasky ] 16:42 -!- Irssi: ##hplusroadmap: Total of 39 nicks [0 ops, 0 halfops, 0 voices, 39 normal] 16:42 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 16:42 -!- Irssi: Join to ##hplusroadmap was synced in 4 secs 16:43 -!- fenn [~fenn@131.252.130.248] has quit [Ping timeout: 246 seconds] 16:43 -!- fenn [~fenn@131.252.130.248] has joined ##hplusroadmap 16:52 -!- mayko [~mayko@71-22-217-151.gar.clearwire-wmx.net] has quit [Ping timeout: 276 seconds] 17:03 -!- mayko [~mayko@71-22-217-151.gar.clearwire-wmx.net] has joined ##hplusroadmap 17:06 < kanzure> what's up with the ping timeouts 17:18 < kanzure> haha, "biopunk book review" 17:18 < kanzure> http://groups.google.com/group/bebobio/msg/887f88ea67e7e92d 17:18 < kanzure> "Some of the 17:18 < kanzure> stories have just enough information to pique one's interest, but not enough 17:18 < kanzure> information to satisfy it." 17:18 < kanzure> "For instance, I would have liked to have heard 17:18 < kanzure> more about Bryan Bishop, whose prolific posts on the DIYbio message boards 17:18 < kanzure> lead me to believe he is the epitome of a biopunk. " 17:18 < kanzure> haha 17:19 -!- epitron [~epitron@unaffiliated/epitron] has quit [Read error: Operation timed out] 17:20 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has quit [Ping timeout: 246 seconds] 17:22 -!- epitron [~epitron@ava.ponzo.net] has joined ##hplusroadmap 17:22 -!- epitron [~epitron@ava.ponzo.net] has quit [Changing host] 17:22 -!- epitron [~epitron@unaffiliated/epitron] has joined ##hplusroadmap 17:38 < kanzure> hi epitron 17:41 < kanzure> ok i posted my "which dna sequencer should i buy?" question 17:41 < kanzure> http://groups.google.com/group/diybio/browse_thread/thread/e72c9bb618f0deb1 17:51 < kanzure> neat.. "Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency" 17:51 < kanzure> http://www.cell.com/cell-stem-cell/abstract/S1934-5909(11)00111-1 17:52 < kanzure> what "This miRNA-based reprogramming approach is two orders of magnitude more efficient than standard Oct4/Sox2/Klf4/Myc-mediated methods." 17:52 < kanzure> well i guess that lives up to the title "Highly Efficient" 17:57 < kanzure> paper: http://diyhpl.us/~bryan/papers2/stem-cells/Highly%20Efficient%20miRNA-Mediated%20Reprogramming%20of%20Mouse%20and%20Human%20Somatic%20Cells%20to%20Pluripotency.pdf 17:57 < kanzure> ack wait 17:57 < kanzure> it gave me the totally wrong paper? 18:19 < kanzure> because pdx doesn't have a subscription. righto. i'll try another box then 18:29 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has joined ##hplusroadmap 18:33 -!- augur [~augur@208.58.6.161] has quit [Remote host closed the connection] 18:34 -!- eridu [debian-tor@gateway/tor-sasl/eridu] has quit [Ping timeout: 246 seconds] 18:55 -!- augur [~augur@pool-173-73-124-245.washdc.east.verizon.net] has joined ##hplusroadmap 19:14 -!- jrabbit [~babyseal@unaffiliated/jrabbit] has quit [Read error: Operation timed out] 19:18 -!- jrabbit [~babyseal@unaffiliated/jrabbit] has joined ##hplusroadmap 19:46 -!- panax [~panax@cpe-72-185-51-10.tampabay.res.rr.com] has quit [Ping timeout: 240 seconds] 20:17 < ybit> hey guys! favor to ask... :) 20:17 < ybit> i need papers... 20:17 < ybit> http://iopscience.iop.org/0953-4075/31/16/009 20:17 < ybit> http://iopscience.iop.org/0953-4075/33/12/309 20:17 < ybit> http://iopscience.iop.org/0953-4075/33/2/310 20:17 < ybit> and more... 20:21 < ybit> http://dx.doi.org/10.1016/j.radphyschem.2003.12.024 20:21 < ybit> http://prl.aps.org/abstract/PRL/v83/i5/p939_1 20:21 < ybit> and that's all 20:40 -!- augur [~augur@pool-173-73-124-245.washdc.east.verizon.net] has quit [Remote host closed the connection] 21:05 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 21:51 < kanzure> ybit: also send the email to getarticles 21:55 < ybit> okay 21:56 < ybit> and it'd be interesting to know which papers are linked to the most in arxiv :) 21:57 < ybit> thanks for the reminder 22:51 < kanzure> so apparently todd, joe and matias each got $40k from the chilean government and are moving down there 22:53 < jrayhawk> whaaat 22:55 < kanzure> heck if i know. 22:55 < kanzure> oh, not you 22:56 < kanzure> the other joe http://www.youtube.com/watch?v=kmTNtSs1UQY 23:07 < cluckj> lol what 23:07 < cluckj> ahahahahahahahahahaha 23:07 < cluckj> why do you have that 23:11 < cluckj> also what are they doing in chile? lava amp stuff? 23:42 -!- jenniside is now known as jenzebubble 23:52 -!- JayDugger [~duggerj@pool-173-74-79-43.dllstx.fios.verizon.net] has joined ##hplusroadmap 23:52 < JayDugger> Good morning, everyone. --- Log closed Sun May 01 00:00:07 2011