--- Log opened Thu Dec 01 00:00:07 2011 02:39 -!- marainein [~marainein@114-198-77-48.dyn.iinet.net.au] has joined ##hplusroadmap 06:35 -!- eudoxia [~eudoxia@r190-135-49-185.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 06:56 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has joined ##hplusroadmap 06:57 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has quit [Client Quit] 07:22 < JayDugger> Good morning, everyone. 07:44 -!- mayko [~mayko@cpe-098-122-166-230.nc.res.rr.com] has joined ##hplusroadmap 08:05 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap 08:08 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has joined ##hplusroadmap 08:09 -!- panax [~panax@131.247.10.245] has joined ##hplusroadmap 08:34 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Ping timeout: 248 seconds] 08:38 -!- Technicus [~Technicus@DSLPool-net208-2.wctc.net] has quit [Quit: KVIrc 4.1.1 Equilibrium http://www.kvirc.net/] 08:43 -!- delinquentme [~asdfasdf@c-76-125-163-60.hsd1.pa.comcast.net] has joined ##hplusroadmap 08:50 < strages> morning 08:53 < kanzure> hi 08:53 < delinquentme> OMG 08:53 < delinquentme> CHECK IT GUYS 08:53 < delinquentme> I GOT A SMERT ANSAR 08:53 < delinquentme> http://www.reddit.com/r/askscience/comments/mvhrz/what_is_the_limiting_factor_in_dna_sequencing/#c3485bf 08:53 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap 08:56 < kanzure> delinquentme: those are some pretty standard answers 08:57 < kanzure> wait, 5000 bps? 08:58 < delinquentme> 5k is the top best of the best we can do 08:58 < delinquentme> dont quote me 08:59 < delinquentme> kanz im looking for this article talking about using microfluidics for chromosomal separation pre sequencing 08:59 < delinquentme> any idea where it might be? 09:01 < kanzure> do you mean sorting of chromosomes or hylicase-based separation 09:01 < kanzure> helicase 09:01 < kanzure> or whatever the enzyme is supposed to be; helicase isn't for chromosomes 09:02 < delinquentme> umm sorting of chromosomes 09:02 < delinquentme> i remember it off of fluidic mems 09:02 < delinquentme> i THOUGHT 09:02 < kanzure> lots of papers about sorting: http://diyhpl.us/~bryan/papers2/microfluidics/ 09:36 -!- mayko [~mayko@cpe-098-122-166-230.nc.res.rr.com] has quit [Quit: Leaving] 09:39 -!- eudoxia [~eudoxia@r190-135-49-185.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving.] 10:58 -!- eudoxia [~eudoxia@r190-135-43-11.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 11:03 -!- eudoxia [~eudoxia@r190-135-43-11.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving.] 12:31 -!- He||eshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has joined ##hplusroadmap 12:31 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has quit [Ping timeout: 260 seconds] 13:17 -!- klafka_ [~textual@cpe-74-74-157-63.rochester.res.rr.com] has quit [Ping timeout: 244 seconds] 13:24 -!- klafka [~textual@cpe-74-74-157-63.rochester.res.rr.com] has joined ##hplusroadmap 13:35 -!- eudoxia [~eudoxia@r190-135-92-112.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 13:46 -!- hymension [~symmetry@134.219.142.155] has joined ##hplusroadmap 13:46 < hymension> home to the nodes of ranvier 13:49 -!- hymension [~symmetry@134.219.142.155] has left ##hplusroadmap ["Leaving"] 13:50 -!- eudoxia [~eudoxia@r190-135-92-112.dialup.adsl.anteldata.net.uy] has quit [Remote host closed the connection] 13:50 -!- eudoxia [~eudoxia@r190-135-92-112.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 13:54 < delinquentme> umm HERROH. 13:54 < delinquentme> IM ROOKING far SomeWAN who knoz how to prep DNA 13:54 < delinquentme> eudoxia, howdah! 14:01 < delinquentme> kanzure, have you ran into any cool microfluidic setups for PCR? 14:03 < kanzure> tons.. yes 14:04 < kanzure> http://diyhpl.us/~bryan/papers2/microfluidics/pcr/ 14:04 < delinquentme> hot. 14:04 < delinquentme> damn. 14:04 < delinquentme> umm DNA quantification 14:04 < kanzure> also this one 14:04 < kanzure> http://diyhpl.us/~bryan/papers2/microfluidics/gkm389%20Miniaturized%20PCR%20chips%20for%20nucleic%20acid%20amplification%20and%20analysislatest%20advances%20and%20future%20trends.pdf 14:04 < kanzure> and since you're hot for dna sequencing stuff: 14:04 < kanzure> http://diyhpl.us/~bryan/papers2/microfluidics/Microfabricated%20bioprocessor%20for%20integrated%20nanoliter-scale%20Sanger%20DNA%20sequencing.pdf 14:05 < kanzure> http://diyhpl.us/~bryan/papers2/microfluidics_Sanger_sequencer.png 14:05 < kanzure> http://diyhpl.us/~bryan/papers2/DNA/High-fidelity%20gene%20synthesis%20by%20retrieval%20of%20sequence-verified%20DNA%20identified%20using%20high-throughput%20pyrosequencing%20-%202010.pdf 14:05 < kanzure> http://diyhpl.us/~bryan/papers2/DNA/Real-time%20DNA%20sequencing%20from%20single%20polymerase%20molecules.pdf is a famous one (by now) 14:11 < delinquentme> kanzure, dna quantification 14:12 < delinquentme> that is the big term for separating out DNA by length right? 14:27 < kanzure> uh? i don't know for sure 14:27 < kanzure> sounds like BS 14:30 < delinquentme> dna quantitation 14:31 < delinquentme> its getting the fluid / DNA saturation just right 14:38 -!- uniqanomaly [~ua@dynamic-87-105-21-181.ssp.dialog.net.pl] has joined ##hplusroadmap 15:05 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Remote host closed the connection] 15:06 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap 15:27 -!- klafka [~textual@cpe-74-74-157-63.rochester.res.rr.com] has quit [Remote host closed the connection] 15:36 -!- panax [~panax@131.247.10.245] has quit [Ping timeout: 252 seconds] 15:49 -!- Mariu [Jimmy98@89.41.57.33] has quit [] 16:18 -!- klafka [~textual@cpe-74-74-157-63.rochester.res.rr.com] has joined ##hplusroadmap 16:27 -!- SDr [SDr@unaffiliated/sdr] has joined ##hplusroadmap 16:33 -!- pasky [pasky@nikam.ms.mff.cuni.cz] has quit [Ping timeout: 252 seconds] 16:33 -!- pasky [pasky@nikam.ms.mff.cuni.cz] has joined ##hplusroadmap 16:42 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has quit [Quit: jmil] 17:05 -!- Technicus [~Technicus@DSLPool-net208-2.wctc.net] has joined ##hplusroadmap 17:12 -!- Fiohnel [~r3idslash@111.94.200.235] has quit [Ping timeout: 240 seconds] 18:11 -!- He||eshin is now known as Helleshin 18:42 -!- He||eshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has joined ##hplusroadmap 18:45 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has quit [Ping timeout: 248 seconds] 19:10 -!- klafka [~textual@cpe-74-74-157-63.rochester.res.rr.com] has quit [Ping timeout: 240 seconds] 19:13 -!- klafka1 [~textual@cpe-74-74-157-63.rochester.res.rr.com] has joined ##hplusroadmap 19:13 -!- delinquentme [~asdfasdf@c-76-125-163-60.hsd1.pa.comcast.net] has quit [Quit: Leaving] 20:38 -!- eudoxia [~eudoxia@r190-135-92-112.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving.] 20:45 -!- Technicus [~Technicus@DSLPool-net208-2.wctc.net] has quit [Quit: KVIrc 4.1.1 Equilibrium http://www.kvirc.net/] 21:50 -!- SDr [SDr@unaffiliated/sdr] has quit [] 22:30 < Utopiah> http://www.roboticopenplatform.org 22:30 < kanzure> do i have to 22:34 < Utopiah> "Robotic Open Platform (ROP) aims to make hardware designs of robots available under an Open Hardware license to the entire robotic community. It provides CAD drawings, electric schemes and the required documentation to build their own robot." 23:05 -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap --- Log closed Fri Dec 02 00:00:09 2011