--- Log opened Fri Feb 17 00:00:14 2012
00:04 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap
00:19 -!- sylph_mako [~mako@118-93-29-111.dsl.dyn.ihug.co.nz] has quit [Read error: Connection reset by peer]
00:33 -!- Stieru_Ridir [~Steel@67.246.36.165] has joined ##hplusroadmap
00:35 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Read error: Operation timed out]
00:35 -!- sylph_mako [~mako@118-93-104-74.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap
00:49 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap
00:50 < rdb> morning Stee|
00:53 -!- Stieru_Ridir [~Steel@67.246.36.165] has quit [Ping timeout: 252 seconds]
00:56 < rdb> the more I learn about electroporators, the easier it seems to build one.  I'm trying to find the catch.
01:43 -!- klafka [~textual@ip-64-139-44-34.dsl.sjc.megapath.net] has joined ##hplusroadmap
01:50 -!- Stieru_Ridir [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap
01:50 < Stieru_Ridir> ugh
01:50 -!- Stieru_Ridir is now known as Steel_
01:52 -!- ThomasEgi [~thomas@pppdyn-74.stud-ko.rz-online.net] has joined ##hplusroadmap
01:52 -!- ThomasEgi [~thomas@pppdyn-74.stud-ko.rz-online.net] has quit [Changing host]
01:52 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap
01:53 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Ping timeout: 240 seconds]
01:55 < Steel_> hey ThomasEgi, how are you?
01:55 < Steel_> rdb as well
01:55 < ThomasEgi> remember when i went to bed?
01:56 < ThomasEgi> was like 5 hours ago, postmen ringed me out of my bed, breakfast still missing.
01:57 < ThomasEgi> well. same as every morning i guess^
01:57 < ThomasEgi> how bout you?
02:00 < Steel_> I was out :P
02:01 < Steel_> I turn 21 tomorrow
02:01 < ThomasEgi> half way to the universal asnwer
02:03 < Steel_> 2 months to salary
02:08 -!- rkos [~chatzilla@a88-113-156-174.elisa-laajakaista.fi] has joined ##hplusroadmap
02:11 < ThomasEgi> one president lesson this planet
02:11 < ThomasEgi> germany's just resigned
02:13 < Steel_> what?
02:13 < Steel_> really?
02:14 < ThomasEgi> yeah.
02:14 < ThomasEgi> like 10 minutes ago
02:15 < rdb> morning ThomasEgi =)
02:15 < ThomasEgi> the guy befor him already resigned
02:15 < ThomasEgi> mornin
02:16 < Steel_> I don't have an opinion on the dude
02:16 < Steel_> dunno enough about him
02:17 < ThomasEgi> politician, wears suit, gets good press, people like him, they put him in charge
02:17 < ThomasEgi> doesnt matter if the person is completely incompetent or corrupt or whatnot
02:18 < ThomasEgi> well.. then the press changed their mind, wrote bad press, people started to hate him.
02:18 < ThomasEgi> now he resigns
02:19 < rkos> does the president in germany do anything?
02:19 < ThomasEgi> signs laws
02:20 < ThomasEgi> other than that he has very little political power. it is mostly representative
02:20 < ThomasEgi> and that earns him bout 200k$ per year, for the rest of his life
02:21 < rkos> so very much like the finnish one
02:23 < rkos> if you don't have a royal house in europe you just elect someone to play an equally pointless role
02:23 < ThomasEgi> and shove money up his ....
02:38 < Steel_> http://www.cenegenics.com/ haha
04:00 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap
04:21 < chris_99> GFP is edible right?
04:27 < marainein> sure. and low carb as well
04:31 < chris_99> nice :) i might see whether i can introduce it into brewers yeast for my homebrew
04:59 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Read error: No route to host]
05:00 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap
05:06 -!- sylph_mako [~mako@118-93-104-74.dsl.dyn.ihug.co.nz] has quit [Ping timeout: 248 seconds]
05:31 -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection]
05:40 -!- _sol_ [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 245 seconds]
06:09 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has quit [Quit: jmil]
06:23 -!- kvltist [~Kvltist@p5B33FA04.dip.t-dialin.net] has joined ##hplusroadmap
06:41 -!- augur [~augur@129.2.129.35] has joined ##hplusroadmap
06:48 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has joined ##hplusroadmap
06:51 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has joined ##hplusroadmap
06:56 < delinquentme> jmil,
06:56 < delinquentme> http://www.neworgan.org/
06:56 < delinquentme> can u run down to the store and grab me a liver?
07:05 < jmil> delinquentme: awesome. is it an open competition?
07:05 < delinquentme> i believe so
07:06 < jmil> the challenge with these types of things is you need millions of dollars to do the research to win the prize...
07:06 < delinquentme> aubrey is continuing to make the prizes
07:06 < jmil> it would be better if they made grants
07:06 < delinquentme> i think its an awesome way to move in the space
07:06 < jmil> gave grants instead of awards
07:06 < jmil> it's very interesting
07:06 < jmil> liver is in progress
07:06 < delinquentme> true but awards is how you make it happen when you're not toting around tons of cash
07:06 < jmil> we just need to survive another 30 years
07:06 < jmil> hmmm
07:07 < jmil> it will take many many millions to implant the organ in a human and follow-up for 2 years
07:08 -!- _sol_ [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap
07:10 < delinquentme> anyone know if its necessarily true that if you work in a gov funded organization you're going to make less than in the private sector?
07:10 < delinquentme> im 3/4ths sure its true
07:14 < jmil> delinquentme: yes that is true
07:14 < jmil> NIH mandates wages and they are publicly available
07:14 < jmil> grad students and postdocs make below minimum wage by the way...
07:15 < delinquentme> hmm
07:15 < delinquentme> i gotta see what lawrence berkley is gonna pay
07:15 < delinquentme> *REALLY* wanna work there
07:15 < delinquentme> but I also **REALLY** want to get paid
07:34 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap
07:44 < kanzure> http://en.wikipedia.org/wiki/Marvel_Anime#Iron_Man
07:44 < kanzure> well my life is now complete, thanks marvel anime
07:52 -!- JonnieCache [~jonnie@87.252.60.35] has joined ##hplusroadmap
08:01 < delinquentme> ^^^
08:01 < delinquentme> kanzure, JonnieCache is my new minion
08:01 < delinquentme> feed him well
08:01 < delinquentme> i've pumped him full of sugared doughnuts and caffeine
08:01 < JonnieCache> lol thanks
08:01 < delinquentme> and now im going to my zumba class
08:02 < JonnieCache> LOL
08:02 < delinquentme> kanzure, runs the show and is like the log.all()
08:02 < JonnieCache> wtf actually is zumba. i only know it as a way of succinctly establishing a character as painfully middleclass in a sitcom script
08:03 < JonnieCache> i realise this is hardly OT
08:03 < delinquentme> lol its like dancing aerobics for middle aged women
08:03 < delinquentme> oh and its a chain operation
08:04 < JonnieCache> aha. well enjoy yourself
08:04 < delinquentme> slightly less interesting than building startups and hardware for the soon to be inflood of necessary raw inputs for synthetic bio
08:05 < delinquentme> #IfExxonWantsIt
08:06 < JonnieCache> fuck exxon. surely the ideal goal is to destroy exxon
08:06 < delinquentme> JonnieCache, the ideal goal is to capitalize on the $$$$ they have
08:06 < delinquentme> in order for us to build a hefty pipeline for engineering bacterial machines
08:06 < JonnieCache> then use that to destroy them? because they certainly dont share your goal of progressing beyond current paradigms.
08:07 < kanzure> what's going on?
08:07 < kanzure> what?
08:07 < delinquentme> I don't really see them as anything more than a conduit
08:07 < delinquentme> a method to what is really worth going after
08:07 < delinquentme> kanzure, pulling optimistic kids in
08:08 < kanzure> kids? if he's over 17 i don't want him
08:08 < delinquentme> talking about exxon and their funding for serious synthetic biology pipeline development
08:08 < delinquentme> hahah
08:08 < JonnieCache> the moment biotech becomes a threat to their model they will try and control/limit it surely
08:08 < delinquentme> JonnieCache, just like the US __TRYING__ to control bit torrents
08:08 < delinquentme> are they succeeding?
08:08 < delinquentme> nope
08:08 < delinquentme> lol
08:08 < delinquentme> let em try
08:09 < delinquentme> they will perpetually be playing catch u
08:09 < delinquentme> up
08:09 < JonnieCache> BT is nothing though. as soon as you start to threaten the structure of the fossil fuel industry youre fucking with dick cheney and his geopolitical vision. he makes the RIAA look like the boy scouts
08:10 < ThomasEgi> shutting down megaload, was cutting traffic for like 5 hours befor people shifted to servers outside the US, adding extra load on transatlantic lines. regulation is futile
08:10 < delinquentme> JonnieCache, hang out here more :D
08:10 < delinquentme> these guys are all big machines operating in a world full of faster crazier kids
08:11 < delinquentme> eventually
08:11 < delinquentme> ( and it is simply a function of time )
08:11 < delinquentme> they will fall
08:11 < JonnieCache> oh yes i fully agree dont worry :)
08:11 < delinquentme> SO
08:11 < delinquentme> we pull in their resources
08:11 -!- _sol_ [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 260 seconds]
08:11 < delinquentme>  get their secrets
08:11 < delinquentme> then suck em dry
08:12 -!- strages [~qwebirc@dev.throwthemind.com] has quit [Ping timeout: 255 seconds]
08:13 < delinquentme> LOLLLLL book of ruby is written with comic sans
08:13 -!- _sol_ [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap
08:13 < JonnieCache> heh which book is this?
08:13 < delinquentme> http://www.sapphiresteel.com/The-Book-Of-Ruby
08:13 < ThomasEgi> how bout a java book in dingbats?
08:13 < delinquentme> looking over dynamic programming atm
08:14 < delinquentme> lolol ThomasEgi aren't they all written in dingbats?
08:14 < delinquentme> PS ThomasEgi howdy :D you seem new
08:14 < ThomasEgi> delinquentme,  i am not :)
08:14 < delinquentme> programmer ? hacker? hardware?
08:14  * delinquentme derps
08:14 < ThomasEgi> all of it
08:14 < JonnieCache> this is the only ruby book you will ever need http://shop.oreilly.com/product/9780596516178.do
08:14 < delinquentme> truck yes :D
08:14 < kanzure> ThomasEgi: you will have to ignore those two
08:15 < delinquentme> http://en.bookfi.org/s/?q=Matsumoto+ruby&t=0  << JonnieCache
08:16 < delinquentme> Its like ...
08:16 < delinquentme> we own the world!
08:16 < kanzure> delinquentme: calm down
08:16 < delinquentme> true true
08:16 < delinquentme> BUT
08:16 < delinquentme> PS kanzure when i land a job in SV
08:16 < delinquentme> im going to be bloody manic for weeks
08:16 < JonnieCache> kanzure: those two? dont lump me in with anyone else ;)
08:17 < delinquentme> yeah im not a solid one to associate w
08:17 < delinquentme> ooo esp in #biology and #bioinformatics haha
08:17 < delinquentme> thats a love / hate relationship there
08:18 < ThomasEgi> anyone knows when yashgaroth will be around again?
08:18 < delinquentme> ThomasEgi, hes usually online later at night?
08:19 < kanzure> ThomasEgi: about seven or eight hours
08:19 < delinquentme> (relative to my time here )
08:19 < kanzure> you guys need to stop getting jobs, and instead get back to work on things that matter
08:20 < delinquentme> kanzure, ha
08:20 < delinquentme> 1 ) income helps
08:20 < delinquentme> 2) im gonna be hanging out w allll kinds of crazy kids when im down there
08:20 < delinquentme> 3) develop the habits to work harder
08:21 < delinquentme> diet is part of that .. housed a half gallon of thin mint icecream last night while watching SC2 streams
08:21 < delinquentme> XD
08:21 -!- strages_work [~qwebirc@dev.throwthemind.com] has joined ##hplusroadmap
08:38 -!- strages_work [~qwebirc@dev.throwthemind.com] has quit [Ping timeout: 245 seconds]
08:40 -!- strages_work [~qwebirc@dev.throwthemind.com] has joined ##hplusroadmap
08:43 < jmil> delinquentme: LBL would be amazing to work at; i've always heard great things. i think they actually pay quite well in general, too. but good science is always more rewarding and fulfilling than more money with a boring job. that's been my MO… so far so good!
08:44 < delinquentme> jmil, good to know .. i've got a call w them later today to see what they're up to .. but I'm wondering what the gap between their pay and private is
08:44 < jmil> delinquentme: what field ?
08:45 < delinquentme> they've got this hella sexy niche position
08:45 < jmil> ??
08:45 < delinquentme> its a bioinformatics programmer for a web application called Kbase which is running on rails
08:45 < jmil> in what
08:45 < jmil> ooh awesome
08:45 < delinquentme> so like super super super niche
08:45 < jmil> lol
08:45 < delinquentme> yeah its synthetic bio + rails + API work + silicion valley
08:46 < jmil> is this it: http://www.systemsbiologyknowledgebase.org/ ?
08:46 < delinquentme> http://www.kbase.us/
08:47 < delinquentme> surely they know that private is going to be paying
08:47 < delinquentme> so yeah thats what im really curious about
08:48 -!- JayDugger [~duggerj@pool-173-74-78-36.dllstx.fios.verizon.net] has joined ##hplusroadmap
08:51 < kanzure> i really doubt they will pay 100k+ for that position
08:51 < kanzure> *maybe* 50k
08:53 < delinquentme> have to see
08:59 < kanzure> this guy has to touch the mouse too often :/
08:59 < kanzure> http://www.youtube.com/watch?v=epRNaSIibew
08:59 < kanzure> time lapse of someone programming
09:11 < delinquentme> kanzure,  #biology
09:11 < delinquentme> cooking old chicken
09:11 < delinquentme> lol
09:42 < delinquentme> if i dont come back Lando gets all my socks
09:44 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Read error: No route to host]
09:45 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap
10:06 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Quit: Leaving.]
10:11 < delinquentme> LAWL
10:11 < delinquentme> http://www.bio-itworld.com/news/02/17/12/Oxford-strikes-first-in-DNA-sequencing-nanopore-wars.html
10:12 < delinquentme> 100k reads @ 900 bones
10:12 < kanzure> bones?
10:12 < delinquentme> haha
10:12 < delinquentme> yeah
10:12 < delinquentme> USD bones
10:39 -!- klafka [~textual@ip-64-139-44-34.dsl.sjc.megapath.net] has quit [Quit: Computer has gone to sleep.]
10:42 -!- klafka [~textual@12.157.117.194] has joined ##hplusroadmap
10:45 -!- augur [~augur@129.2.129.35] has quit [Remote host closed the connection]
10:55 -!- klafka [~textual@12.157.117.194] has quit [Quit: Computer has gone to sleep.]
11:24 -!- sylph_mako [~mako@118-93-13-165.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap
11:32 -!- sylph_mako [~mako@118-93-13-165.dsl.dyn.ihug.co.nz] has quit [Ping timeout: 260 seconds]
11:45 -!- sylph_mako [~mako@118.93.192.123] has joined ##hplusroadmap
11:59 -!- sylph_mako [~mako@118.93.192.123] has quit [Ping timeout: 260 seconds]
12:16 -!- sylph_mako [~mako@118.93.192.123] has joined ##hplusroadmap
12:21 -!- yottabit [~heath@unaffiliated/ybit] has joined ##hplusroadmap
12:33 < chris_99> has anyone used luciferin before?
12:34 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has quit [Ping timeout: 240 seconds]
12:47 -!- kvltist [~Kvltist@p5B33FA04.dip.t-dialin.net] has quit [Ping timeout: 265 seconds]
12:54 -!- yash-phone [~yaaic@66-87-4-14.pools.spcsdns.net] has joined ##hplusroadmap
12:54 < yash-phone> oi thomasegi i'm here if you wanna talk, but i'm on mein handy so i can't type too well
12:55 -!- Steel_ [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Read error: Connection reset by peer]
12:55 < ThomasEgi> yash-phone, just wanted to let you know that the it would be possible to go up to 1500V for that circuit.
12:55 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap
12:56 < yash-phone> oh man 600 is like beyond maximum; as long as it can do 1 amp at that voltage i'll be fine
12:58 < yash-phone> though it might be good to have 1kilovolt if we can get the pulse duration under ~10microseconds
13:04 < ThomasEgi> the parts can withstand a maximum of 8A
13:04 < ThomasEgi> continous current. peak current usualy is higher
13:09 -!- augur [~augur@129.2.129.35] has joined ##hplusroadmap
13:10 < chris_99> are you making an electroporator?
13:11 < chris_99> oh the voltage would be far too high for that heh
13:16 < yash-phone> it is an electroporator, just not for bacteria because those are boring and easy
13:17 < yash-phone> in vivo human muscle is the target for this one
13:18 < chris_99> oh interesting, so the voltage is a lot higher then
13:18 < chris_99> because its only ~2V for bacteria right?
13:22 < yash-phone> no you have it inverted
13:23 < yash-phone> bacteria are kilovolts due to their smallness, we won't be going over 200V/cm
13:24 < yash-phone> also bacteria have cell walls and shit, and while no one cares about bacterial survival, we want low toxicity for muscle fibers
13:25 < chris_99> is wikipedia wrong then: 'It is generally accepted that for a given pulse duration and shape, a specific transmembrane voltage threshold exists for the manifestation of the electroporation phenomenon (from 0.5 V to 1 V).'
13:27 < yash-phone> "several hundred vollts across a distance of several millimeters are typically used"
13:28 < yash-phone> just a little bit further down the page
13:28 < kanzure> "(e.g. Merck's recently announced scientific staff cuts being moved to China)"
13:28 < chris_99> but surely the bit i just posted is wrong
13:28 < chris_99> then
13:28 < yash-phone> i'm no electronicist, but it's phrased badly
13:29 < chris_99> i'm curious about making one of these using a cockroft-walton circuit then
13:29 < chris_99> and a cap bank
13:29 < yash-phone> heh cockroft
13:29 < yash-phone> sorry, continue
13:29 < chris_99> heh, well its a way to convert AC to high voltage dc
13:29 < chris_99> then i could use a cap bank to get the ampage
13:30 < chris_99> presuming it requires high amps
13:30 < rdb> they aren't very accurate
13:30 < chris_99> hmm, i could possibly get a hv zener i presume then
13:30 < rdb> inaccuracies in every stage build up while with electroporation you'll want a pretty reliable way of producing the correct voltage
13:31 < rdb> do you only need a fixed voltage?
13:31 < chris_99> a high voltage dc pulse i believe then
13:32 < rdb> you can use a high-voltage boost converter to get up to kV range, I think Thomas' design uses one of those
13:32 < chris_99> not heard of a HV boost converter
13:32 < chris_99> only low voltage ones
13:34 < chris_99> sorry ignore that
13:34 < chris_99> as boost is probably a generic term for any voltage increasing device
13:34 < rdb> indeed
13:34 -!- eudoxia [~eudoxia@r190-135-15-48.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap
13:34 < chris_99> i just bought some low voltage 150W ones which is why i said that
13:35 < chris_99> what ampage do you need for an elctroporator yash-phone?
13:36 < yash-phone> for mine, 1; i don't follow specifics for bacteria much
13:37 < chris_99> sure whats the ampage for yours then
13:37 < rdb> 1 A at 1 kV is 1000 W
13:37 < chris_99> and the pulse length?
13:38 < yash-phone> um 10-50 milliseconds
13:38 < yash-phone> i'm not doing 1000v, more like 200 at most
13:39 < rdb> components that go up to 200 v are pretty cheap
13:39 < rdb> it'd be quite simple to make that
13:39 < rdb> actually you can make it run off mains power that way, without need for any transformation
13:39 < rdb> and switch using a highspeed relay
13:39 < yash-phone> also all these settings need to be adjustable
13:40 < yash-phone> i outlined it last night in here, but am on my phone so im not gonna copy/paste it
13:40 < chris_99> ah ok
13:40 < chris_99> you could try simulating in LTSpice
13:41 < chris_99> you could also probably use a transistor
13:41 < chris_99> rather than a relay
13:45 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has joined ##hplusroadmap
13:48 < rdb> I've found high-voltage transistors up to 1600 V or so
13:49 < ThomasEgi> 1500 transistors are pretty common, and 80 cent cheap
13:51 < chris_99> nice :)
13:55 -!- Stieru_Ridir [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap
13:56 -!- Steel_ [81a14818@gateway/web/freenode/ip.129.161.72.24] has joined ##hplusroadmap
13:56 < Steel_> how goes
13:59 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Ping timeout: 240 seconds]
13:59 < eudoxia> yo steel
13:59 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has joined ##hplusroadmap
14:00 < Steel_> hmm
14:00 < Steel_> I guess my account at home got disconned
14:04 < rdb> oh, morning
14:05 < rdb> heading to bed, good night
14:07 < archels> kanzure: Any idea where to get an XY or XYZ micromanipulator for the cheap?
14:08 < kanzure> what does a micromanipulator mean in this context.. are you looking for servos and steppers
14:09 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Read error: Connection reset by peer]
14:09 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap
14:12 < Steel_> if you want to do microfluidic assembly, I have some links for how to build one
14:12 < chris_99> linky?
14:13 < Steel_> ftp://ftp.eng.umd.edu/:/home/enae/faculty/benshap/pub/papers/ArmaniChaudharyProbstShapiro_FeedbackParticleSteering_JMEMS_Aug06.pdf this series of papers
14:15 < chris_99> don't you need expensive equipment to actually fab it?
14:15 < Steel_> hmmm
14:15 < Steel_> I don't think so
14:22 -!- yash-phone [~yaaic@66-87-4-14.pools.spcsdns.net] has quit [Ping timeout: 252 seconds]
14:26 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap
14:27 < Steel_> yay autist arguments in LW
14:27 < kanzure> http://www.newscientist.com/article/dn21495-usb-stick-can-sequence-dna-in-seconds.html?DCMP=OTC-rss&nsref=online-news
14:27 < kanzure> oh it's oxford nano
14:27 < kanzure> "Each unit is expected to cost $900 when it goes on sale later this year."
14:27 < kanzure> "This device will be capable of sequencing a human genome in 2 hours for around $1000, Rothberg claims."
14:28 < kanzure> hrmmm
14:29 < archels> kanzure: That would be fine, but I've also spotted manual, mechanical ones.
14:30 -!- Stieru_Ridir [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Ping timeout: 240 seconds]
14:31 < chris_99> wow
14:31 < chris_99> that looks amazing
14:33 < ParahSailin> wow nanopore sequencing
14:33 < ParahSailin> i feel bad for those halcyon molecular guys
14:34 < kanzure> nah their real business plan is "sell microscopes"
14:34 < ParahSailin> but they were kinda douchey anyway
14:34 < kanzure> i interviewed with them.. their plans are a little shakey
14:34 < kanzure> "SEQUENCE EVERYTHING and hope we live forever; or just sell microscopes if someone beats us to market"
14:35 < eudoxia> the money will fund Randal Koene's volcano fortress
14:35 < chris_99> oh are you in the UK then kanzure?
14:35 < Steel_> he's talking about halcyon in san fran
14:36 < kanzure> chris_99: no
14:36 < kanzure> eudoxia: it'll fund randal's illegitimate kiddos, that's what it'll do
14:36 < chris_99> aren't they a UK firm though
14:36 < kanzure> oxnano is. halcyon molecular is not.
14:36 < chris_99> ah sorry
14:37 < kanzure> http://google.com/search?q=randal+koene+rapechild
14:37 < eudoxia> but I sort of liked randal koene
14:37 < Steel_> wonder what's going on locally...
14:37 < Steel_> I know this district has a huge amount of nano
14:37 < kanzure> yeah he's fine, i'm just being unfair
14:37 < kanzure> it's not really a rapechild
14:37 < kanzure> just some crazy mom
14:37 < Steel_> most firms in the country, for nanomaterials, etc.
14:37 < eudoxia> is this related to the blog of some crazy person who had an obsession over some guy at Halcyon?
14:37 < eudoxia> there was a blog about it, something like that
14:37 < kanzure> yes
14:37 < eudoxia> ah
14:37 < kanzure> basically, he put his dick in crazy i think
14:38 < Steel_> eudoxia: You should clearly go to CSNE
14:38 < kanzure> so anyway, i thought that was funnier than volcano fortress
14:38 < ParahSailin> wtf rapechild?
14:38 < kanzure> hahah
14:38 < roksprok> i kind of want a hitler bot though
14:39 < ParahSailin> i just remember that luke and that fat guy at halcyon were particularly douchey
14:39 < kanzure> well, they don't have to be nice with that much dough
14:39 < kanzure> :/
14:39 < roksprok> are they like, trying to work on uploading too?
14:39 < kanzure> nope
14:39 < eudoxia> ssshhhhh
14:39 < kanzure> unless they lied to me
14:40 < Steel_> http://cnse.albany.edu/AboutUs/FacultyStaff/Faculty.aspx any good names on this list kanz?
14:40 < roksprok> i'd kind of like to know what koene does all day
14:40 < roksprok> like from carboncopies.org it looks like he's designing nanobots
14:40 < eudoxia> it would be hilarious if they'd blown all the money on ATLUMs and were trying to upload some random hobo
14:40 < roksprok> and i guess....microscopes help with those?
14:41 < kanzure> possibly tenenbaum
14:41 < Steel_> Hobo?
14:41 < roksprok> the only overlap i can find is molecular ticker tape to read neural synapses
14:41 < Steel_> Goertzal
14:41 < eudoxia> oh I don't know
14:41 < eudoxia> who else?
14:41 < kanzure> really he just spends time doing data analysis on their reads
14:41 < eudoxia> it takes at least three years to cut up a brain and the Yudkowsky and Goertzel are still active from what I can tell
14:41 < eudoxia> not descerebrated corpses. yet
14:45 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has quit [Quit: jmil]
14:46 < Steel_> Visiting #lw again reminds me why I stopped going there and started hanging here in the first place
14:54 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Quit: Leaving.]
14:55 < ParahSailin> whys that
14:56 < Steel_> it's impossible to have a conversation that doesn't segue into branches and sub branches about what you mean and what you really mean and status signalling and signal/noise ratio and all sorts of other bullshi
14:56 < Steel_> *bullshit
14:56 < roksprok> i think its just a different type of crowd
14:56 < Steel_> I mean, I like some of those dudes
14:57 < Steel_> and I'll probably go to a couple LW DC meetups
14:57 < roksprok> like, people there think sorting algorithms are neat and like to argue about the best one or whatever....but if you don't really give a shit about the algorithm and just want a simple way to sort stuff that can be tiring
14:58 < Steel_> bueh, I'm more talking about just the ability to hold a conversation in general
14:58 < roksprok> yea its kind of funny that they write so much about signaling, but they just end up doing it more
14:58 < roksprok> and to less productive measures
14:59 < Steel_> they didn't like my solution to akrasia >_>
14:59 < Steel_> 'Stop bitching and make yourself work'
14:59 < ParahSailin> heh
15:00 < ParahSailin> http://milkwood.net/2009/07/27/how_to_build_a_geodesic_chook_dome/ thats pretty neat
15:04 < ParahSailin> yah i guess i dont really like the culture of people who come from lesswrong
15:04 < roksprok> ParahSailin: agreed...i think i recall hearing that 20% of free range birds were lost to predation
15:04 < roksprok> this solves that, at least for a small farm
15:04 < ParahSailin> i've been on some objectivist forums and chatrooms and lesswrong trolls were the worst
15:05 < ParahSailin> free range is wasteful anyway, confinement maximizes pasture yield
15:05 < ParahSailin> maximizes eating to trample ratio
15:05 < Steel_> natural meat is dumb
15:06 < Steel_> vat grown all the way (soooon)
15:06 < roksprok> wouldn't confinement in factory farms maximize it even more?
15:06 < ParahSailin> confinement in a chicken house is bad for the health of the bird
15:06 < ParahSailin> so inferior meat
15:06 < ParahSailin> but yes, yields are maximized there
15:06 < Steel_> vat grooooown
15:07 < roksprok> ah....i did not realize that chicken was noticeably worse from a factory farm
15:07 < eudoxia> big, fat, undifferentiated chunks of muscle oh yes
15:07 < Steel_> eudoxia: the vat grown?
15:07 < eudoxia> yeah
15:07 < roksprok> Stee|_: vat grown would be awesome, if it didn't cost hundreds of thousands a pound
15:07 < Steel_> tailored leanness to meat
15:07 < Steel_> cheap goat
15:08 < Steel_> :D
15:08 < Steel_> they think they've come up with a way to make it marketable
15:08 < eudoxia> well they better
15:09 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has quit [Ping timeout: 240 seconds]
15:16  * eudoxia goes to eat morally reprehensible food
15:17 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 240 seconds]
15:21  * ThomasEgi spend 9 hours, trying to figure out why this darn piece of microcontroller software wouldnt work.
15:22 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has joined ##hplusroadmap
15:22 < ThomasEgi> turned out an array-index was wrong. should've been 10, was 9.
15:22  * ThomasEgi headdesks
15:29 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap
15:37 -!- Steel_ [81a14818@gateway/web/freenode/ip.129.161.72.24] has quit [Ping timeout: 245 seconds]
15:46 < eudoxia> ah yes the good ol' off-by-one error
15:47 < ThomasEgi> really sad
15:47 < ThomasEgi> well. at least i get payed for it
16:08 < Stee|> ThomasEgi, you do freelance?
16:14 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving]
16:27 < ThomasEgi> Stee|, running my own bussiness.
16:44 < Stee|> cool
17:14 -!- sylph_mako [~mako@118.93.192.123] has quit [Ping timeout: 252 seconds]
17:16 -!- klafka [~textual@12.157.117.194] has joined ##hplusroadmap
17:16 < ThomasEgi> well. it still is mostly timeconsuming work, somtimes no fun.. but at least better than spending a 40h week in some crappy office
17:18 < Stee|> hah
17:18 < Stee|> I'm looking forward to my 40+ hour a week job
17:18 < Stee|> because it's interesting shit that will teach me a bunch
17:18 < Stee|> it pays well
17:18 < Stee|> I get to go to europe and other parts of the country more
17:18 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has joined ##hplusroadmap
17:18 < Stee|> and I get to network with mil/ind types and try to bring more h+ stuff to prominence
17:19 < delinquentme> http://www.youtube.com/watch?feature=player_embedded&v=VxSs1kGZQqc#!
17:19 < delinquentme> way cool
17:20 < Stee|> hmm
17:20 < Stee|> could you do this with EDM?
17:21 < Stee|> I wonder how expensive that equipment is
17:26 < kanzure> http://pathogenomics.bham.ac.uk/blog/2012/02/oxford-nanopore-megaton-announcement-why-do-you-need-a-machine-exclusive-interview-for-this-blog/
17:30 < Stee|> http://semiaccurate.com/2012/02/16/danish-researchers-invent-2nm-components/ eudoxia
17:30 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap
17:31 < eudoxia> graphene is <3
17:36 < kanzure> heh http://chrisyeh.blogspot.com/2012/02/reverse-demo-day-thursday-february-23.html
17:36 < kanzure> angels pitching to entrepreneurs
17:38 < Stee|> gimme 3 years :-/
17:40 < roksprok> don't angels get enough pitches already?
17:40 < roksprok> like the point of a demo day is to have investors contact you
17:41 < roksprok> is there really such a frenzy that angels need to beg companies to let them give them money?
17:42 < kanzure> investors also compete between each other
17:42 -!- eudoxia [~eudoxia@r190-135-15-48.dialup.adsl.anteldata.net.uy] has quit [Read error: Connection reset by peer]
17:42 < Stee|> *for good startups
17:42 < Stee|> as opposed to shit startups
17:43 < kanzure> frankly i think poop and poop-related startups will really take off in the genomic era
17:43 < kanzure> sequence your poop in 20 seconds or less
17:44 < ThomasEgi> flush it in less than 5
17:46 -!- yashgaroth [~f@cpe-24-94-5-223.san.res.rr.com] has joined ##hplusroadmap
17:59 -!- eudoxia [~eudoxia@r186-52-146-36.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap
17:59 -!- CIA-92 [~CIA@cia.atheme.org] has joined ##hplusroadmap
18:03 -!- Netsplit *.net <-> *.split quits: qnm, CIA-9
18:08 < kanzure> hi yashgaroth
18:09 < yashgaroth> oh hi kanzure
18:09 < kanzure> nathan is redoing his diagram apparently
18:09 < kanzure> did you see the oxnano announcement
18:09 < yashgaroth> will believe when I use one
18:10 < kanzure> agreed. they claim later this year *cough*
18:10 < yashgaroth> when your device costs as much as your per-genome reagent cost, something seems fishy
18:12 < yashgaroth> still, it reminds me of the g-reader from transmet, so I can't rag on it too much
18:16 < kanzure> "Berger suggested that Sigma produce the compound on a larger scale and taught his process to Sigma personnel. A small ad in a scientific journal brought orders and Broida expanded production. Within two years, Sigma offered eight additional ATP derivatives and raised purity levels"
18:16 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection]
18:17 < yashgaroth> ?
18:17 < kanzure> "Aldrich offered 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) as its first product, widely used as a methylating reagent. Other products offered in the early 50s include 3-hydroxypyridine, which later became one of Aldrich’s best-selling products; ethyl diazoacetate; tetranitromethane; and ethanedithiol."
18:18 < kanzure> just wondering how merck/sigma/aldrich got started
18:18 < kanzure> an ad in a journal? really now?
18:19 < kanzure> $2B in revenue in 2008?
18:19 < kanzure> "Approximately one million individual customers worldwide" uhuh..
18:20 < yashgaroth> who, sigma? they've p. much become the standard for a lot of reagents
18:21 < kanzure> sure
18:21 < kanzure> but,
18:21 < kanzure> let's say they have 20,000 chemicals they manufacture
18:21 < kanzure> i can't see how they have enough customers buying those chemicals to warrant separate production facilities
18:21 < kanzure> consider the 100s of buildings that merck has in texas... all for production.
18:22 < yashgaroth> still nothing compared to, say, dow
18:22 < yashgaroth> or whatever goes on in new jersey with those thousands of chemical silos
18:25 < yashgaroth> and anyone the size of merck needs a few dozen buildings to hide the bodies of all the conspiracy theories trying to find the hidden cure for cancer
18:26 < yashgaroth> theorists*
18:26 < kanzure> conspiracy theories trying to find the hidden cure for cancer theorists, got it :)
18:26 < kanzure> pesky cancer theorists
18:27 < yashgaroth> there's, like, levels...man
18:28 < roksprok> speaking of cancer...does anyone know about pink army cooperative?
18:28 < roksprok> their website is kind of vague
18:28 < kanzure> roksprok: i've written a bit of software for pink army
18:28 < kanzure> and i know andrew pretty well.
18:28 < kanzure> what would you like to know?
18:29 < roksprok> do they have a research staff / lab space?
18:29 < kanzure> no
18:29 < kanzure> it's just andrew hessel going around giving presentations
18:30 < roksprok> i think i saw they have about 500-600 signups, is that about right?
18:30 < roksprok> any other source of funds?
18:30 < kanzure> http://www.youtube.com/results?search_query=andrew+hessel
18:30 < kanzure> nope no other money
18:30 < roksprok> thanks, i'll watch some of the videos
18:31 < kanzure> uhrm. why?
18:31 < kanzure> i mean, what are you looking for
18:32 < roksprok> mainly, is stuff really that cheap?
18:32 < yashgaroth> "Not being profit-driven frees up Pink Army to focus on finding cures that work" ...what?
18:32 < roksprok> like i know diybio has made a lot of cheaper lab gear
18:33 < roksprok> but it seems like genetic engineering of viruses, even if you just want to try it on lab animals, would still be kind of out of reach
18:33 < kanzure> roksprok: ok so you're looking for a general diybio overview.. i se
18:33 < kanzure> *see
18:33 < kanzure> andrew doesn't really get into specifics like that
18:33 < kanzure> but he talks pretty well about possibilities and sometimes what we currently know about biology
18:33 < kanzure> think of his presentations more like "Here's a bunch of trends" heh'
18:33 < roksprok> ah ok
18:34 < kanzure> if you're interesed in gene therapy (w/ viruses) research,
18:34 < kanzure> see: http://diyhpl.us/~bryan/papers2/gene-therapy/
18:35 < roksprok> thanks
18:35 < roksprok> diyhpl.us has been super helpful actually
18:35 < roksprok> and the ezproxy link you posted a little while ago
18:35 < roksprok> i actually just ordered parts to do a little experiment
18:35 -!- SDr [~SDr@unaffiliated/sdr] has joined ##hplusroadmap
18:36 < kanzure> what are you planning?
18:36 < roksprok> trying to reproduce maze learning in nematodes
18:36 < roksprok> er...c elegans
18:36 < roksprok> http://microfluidics.utoronto.ca/papers/worm%20learning.pdf
18:36 < roksprok> that's the paper that described it
18:36 -!- eudoxia [~eudoxia@r186-52-146-36.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving]
18:37 < roksprok> i'm going to try the shrinky-dink method to make the mazes
18:37 < kanzure> ah okay
18:37 < roksprok> the channels can be 1mm wide
18:37 < kanzure> well, sounds fun
18:37 < roksprok> so its really only borderline microfabrication
18:37 < kanzure> i should get around to microfluidic-dinoflagellate-droplet-stuff
18:39 < roksprok> the microfluidic stuff in general has been really fascinating, especially because i had pretty much no idea about it a few weeks ago
18:39 < roksprok> also, i'd like run something else by you as kind of a longer term goal
18:40 < roksprok> single neuron recording is pretty well established
18:40 < kanzure> sure.. patch clamp techniques
18:40 < roksprok> would it be possible to do that in c elegans?
18:40 < roksprok> in a highly parrallel manner
18:40 < roksprok> and combine it with the connectome to 'emulate' one?
18:41 < kanzure> what?
18:41 < kanzure> did you see the nematode upload project?
18:42 < roksprok> http://code.google.com/p/openworm/
18:42 < roksprok> that?
18:42 < kanzure> nope
18:42 < roksprok> or the one guy named david from mit/harvard?
18:43 < kanzure> nope. david's stuff is actually more recent, but i don't recall him making any progress
18:43 < roksprok> http://lesswrong.com/lw/88g/whole_brain_emulation_looking_at_progress_on_c/
18:43 < roksprok> that has all the projects i know about
18:43 < kanzure> http://web.archive.org/web/20070814044636/minduploading.org/research/data.nematodeupload.html
18:44 < kanzure> "A collaboration led by Eugene Leitl. Its aim is to achieve the 3D reconstruction and functional emulation of individual specimens of C.elegans from scanned images of the vitrified original."
18:46 < roksprok> did he have much success? or is it still ongoing?
18:46 < kanzure> there was a gif somewhere, can't find it at the moment
18:46 < kanzure> it showed their reconstruction progress
18:47 < kanzure> anyway, todd huffman's company (3scan) is doing similar things these days
18:47 < kanzure> http://www.3scan.com/
18:47 < kanzure> "3Scan uses the cutting edge technology of the KESM (Knife Edge Scanning Microscope) to automatically section and image samples. Knife-edge scanning, introduced in the KESM instrument, not only preserves image registration throughout the depth of the specimen block but also isolates the tissue above the knife from that below to eliminate undesirable events (back-scattering of light and bleaching of fluorescent-stained tissue below the knife)."
18:47 < kanzure> "Knife-edge scanning supports all known forms of microscopy (absorption imaging using transmitted light, and reflected light imaging using bright-field, dark-field, DIC, and GFP fluorescence)."
18:47 < roksprok> oh wow i thought that was dead
18:47 < kanzure> "sing the KESM, 3Scan is able to scan a one cc^3 block of tissue (for example, a whole mouse brain) at submicron resolution in under 100 hours. With this resolution (a voxel size of 0.6 um x 0.7 um x 1.0 um) 3Scan is able to show cell scale phenomena in the context of a compete organ. "
18:48 < kanzure> "In the example of a whole mouse brain this means that 3Scan is able to accurately model every neuron in the mouse brain sample."
18:48 < roksprok> i think i saw their site like a year ago, they really updated it
18:48 < kanzure> oh he finally published his damn videos http://www.3scan.com/movies/
18:48 < roksprok> they went to chile, i think
18:49 < roksprok> are you able to play the non-jove videos?
18:49 < fenn> no, 3scan is alive and well in san francisco
18:49 < kanzure> nah i haven't bothered. i hate flash.
18:49 < kanzure> i have copies of the videos anyway
18:50 < roksprok> fenn: that's good
18:50 < roksprok> do you have them uploaded somewhere?
18:50 < kanzure> get them yourself..
18:50 < kanzure> http://3scan.com/wp-content/uploads/2011/11/05_Pyramidal_Cells_Golgi.flv
18:51 < roksprok> thanks
18:51 < fenn> oh they have a blog now? about damn time sheesh
18:51 < kanzure> http://3scan.com/wp-content/uploads/2011/11/04_Purkinje_Cells_Golgi.flv
18:51 < kanzure> http://3scan.com/wp-content/uploads/2011/11/09_Mouse_Brain_Vasculature_xvid.flv
18:51 < kanzure> http://3scan.com/wp-content/uploads/2011/11/02_KESM_Cutting_Session.flv
18:51 < roksprok> i feel dumb...it didn't occur to me to try that
18:52 < roksprok> its even exactly where it said it would be
18:52 < kanzure> fenn: you should go sucker punch him for using flash anyway
18:58 < kanzure> gah power went out.
18:58 < roksprok> do you have 3g?
18:59 < roksprok> or...how are you still online?
19:00 < kanzure> sheer will power?
19:00 < roksprok> nice
19:01 < roksprok> and to think i wasted all this money on electricity
19:01 < kanzure> i use a remote server for irc
19:03 < roksprok> i'm going to ignore that and just believe you're black lightning
19:04 < roksprok> what's eugen leitl up to now?
19:05 < kanzure> wife, kids, full-time job
19:05 < roksprok> back to chemistry?
19:06 < roksprok> or still neuroscience/uploading
19:06 < kanzure> some corporate job
19:11 < roksprok> at least the images and stuff are still up
19:11 < roksprok> all hail waybackmachine
19:13 -!- klafka [~textual@12.157.117.194] has quit [Quit: Computer has gone to sleep.]
19:14 -!- augur [~augur@129.2.129.35] has quit [Remote host closed the connection]
19:18  * fenn puts on ninja suit and goes off to steal the internet archive
19:18 < roksprok> shoot let me download this stuff first
19:18 < fenn> srsly why no internet archive torrent
19:22 < kanzure> it would be cool if their church was using wpa or something easily cracked
19:22 < kanzure> then just wire up huge antennas at night on the side of the building
19:24 < kanzure> or, you know.. just intern there
19:25 < Mokbortolan_> spray on antennas
19:32 -!- SDr__ [~SDr@rrcs-50-74-40-146.nyc.biz.rr.com] has joined ##hplusroadmap
19:32 -!- SDr [~SDr@unaffiliated/sdr] has quit [Disconnected by services]
19:32 -!- SDr__ is now known as SDr
19:35 -!- SDr__ [~SDr@rrcs-50-74-40-146.nyc.biz.rr.com] has joined ##hplusroadmap
19:35 -!- SDr [~SDr@rrcs-50-74-40-146.nyc.biz.rr.com] has quit [Disconnected by services]
19:35 -!- SDr__ is now known as sdr
19:38 -!- sdr [~SDr@rrcs-50-74-40-146.nyc.biz.rr.com] has quit [Client Quit]
19:55 -!- eudoxia [~eudoxia@r186-52-146-36.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap
20:09 -!- SDr [~SDr@unaffiliated/sdr] has joined ##hplusroadmap
20:12 -!- SDr [~SDr@unaffiliated/sdr] has quit [Client Quit]
20:16 < uniqanomaly> internet scale distributed file system for backup/storage of all the shit
20:17 < uniqanomaly> replication, fault tolerance etc
20:17 < uniqanomaly> backup of internets
20:18 < uniqanomaly> place to store papers from paywalled publishers
20:18 < uniqanomaly> and nothing ever dies
20:19 < uniqanomaly> Freenet is slow :>
20:24 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has quit [Ping timeout: 240 seconds]
20:31 -!- eudoxia [~eudoxia@r186-52-146-36.dialup.adsl.anteldata.net.uy] has quit [Remote host closed the connection]
20:34 < kanzure> are there any known mirrors of ftp://ftp.ncbi.nlm.nih.gov/genomes/
20:47 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap
20:51 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap
21:10 < kanzure> The Science for Life Extension Foundation puts out a great many of these things, not all of which get translated. The Russian language originals can largely be found at the following sites:
21:10 < kanzure> http://aging-genes.livejournal.com/ (researcher's blog, digging required)
21:10 < kanzure> http://www.scienceagainstaging.org/Edition/edition.html
21:10 < kanzure> For a limited set of English-language translations:
21:11 < kanzure> http://www.scienceagainstaging.org/ENG/index_ENG.html (links at bottom of page)
21:11 < kanzure> http://www.fightaging.org/archives/2010/06/a-regenerative-medicine-roadmap-from-the-science-for-life-extension-foundation.php
21:11 < kanzure> http://www.fightaging.org/archives/2011/07/biomarkers-of-aging-and-age-related-conditions.php
21:11 < kanzure> http://mariakonovalenko.wordpress.com/2012/01/14/telomeres-and-aging-chart/
21:11 < kanzure> http://www.fightaging.org/pdf/regenerative_medicine_roadmap_poster_1.1.pdf
21:13 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Read error: Connection reset by peer]
21:14 < kanzure> this chart just seems so old now http://diyhpl.us/~bryan/papers2/longevity/causes-of-aging.jpg
21:15 < kanzure> http://www.genome.gov/pages/der/sequencing_cost.xls
21:15 < kanzure> http://www.genome.gov/sequencingcosts/
21:26 -!- klafka [~textual@12.157.117.194] has joined ##hplusroadmap
21:27 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap
21:40 < JayDugger> Good evenung, everyone.
21:40 < JayDugger> evening, rather.
21:41 < JayDugger> No more typing for me until I finish a cup of coffee.
21:43 < uniqanomaly> Good sunrise lol
21:45 -!- yottabit [~heath@unaffiliated/ybit] has quit [Read error: Connection reset by peer]
21:46 < kanzure> http://www.fightaging.org/pdf/SCIENCE_AGAINST_AGING_POSTER.pdf
21:46 < kanzure> http://www.fightaging.org/pdf/SAconf.pdf
21:46 < kanzure> http://www.fightaging.org/pdf/SCIENCE_AGAINST_AGING_RESEARCH_PLAN.pdf
21:48 < ParahSailin> kick aging in his testicles! yah, america
21:49 -!- klafka [~textual@12.157.117.194] has quit [Quit: Computer has gone to sleep.]
21:57 < kanzure> anyone know of a case of rational protein design or evolution for increased protein mass?
21:57 < yashgaroth> um, not sure how you mean
21:58 < yashgaroth> you could fuse it to another protein to add mass, I guess
21:58 < kanzure> well, i imagine you could add lots of amino acids to polymerase that would increase its size
21:58 < kanzure> you could maybe add one amino acid at a time until you break something / let it replicate a few thousand times over until it makes the most use of each additional amino acid you add
21:58 < yashgaroth> what use would it get out additional residues exactly
21:58 < kanzure> i'm just thinking, a 500x bigger polymerase might be interesting
21:59 < kanzure> no, we'd get use out of it by being able to grab on to it more easily
21:59 < yashgaroth> I'm pretty sure there's tagged polymerase for that
22:00 < kanzure> well, sure.
22:00 < yashgaroth> if you add a linker like poly-GSGG that doesn't interfere with the function, you can stick on as much as you want to that
22:00 < kanzure> "make it bigger" is just a strategy i guess
22:00 < kanzure> yes i'm familiar with various linkers that work well
22:00 < kanzure> but! mechanical/physical solutions also have their uses
22:01 < yashgaroth> so you want it bigger so it won't wash out through pores I assume?
22:01 < kanzure> i have no particular use case in mind
22:02 < kanzure> "Titin is the largest known protein." http://en.wikipedia.org/wiki/Titin
22:02 < yashgaroth> also making it 500x bigger would also make its on/off extremely slow, even if it theoretically retains its active site etc
22:02 < kanzure> looks like 34,000 amino acid residues
22:02 < yashgaroth> yep titin's big
22:03 < kanzure> yeah i imagine a jumbo polymerase would begin to be selected for sizes/functionality that facilitate moving dna to the right spot
22:03 < kanzure> erm, that is, if you can force polymerase to accept its larger state :P
22:03 < kanzure> obv. the smaller forms are at a local maxima otherwise they wouldn't be so popular
22:04 < yashgaroth> at that point a multi-protein complex tends to be a better plan
22:04 < kanzure> i'm sorta procrastinating.. avoiding some broken javascript
22:04 < yashgaroth> did you try hitting it
22:05 < yashgaroth> that usually fixes things for me
22:05 < kanzure> multiple times
22:05 < yashgaroth> hmm then I'm out of ideas
22:05 < kanzure> i guess protein chemistry is probably different at a 500x scale
22:06 < yashgaroth> interactions rely a lot on random action - a dna-pulling subunit has to be more efficient than just waiting for pol to happen upon the correct place
22:07 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 248 seconds]
22:13 -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has joined ##hplusroadmap
22:14 -!- Mokbortolan_ [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Ping timeout: 240 seconds]
22:19 < kanzure> yashgaroth: yeah but a dna pulling subunit still has to find it
22:19 < kanzure> 'ind'
22:19 < kanzure> *'find'
22:19 < yashgaroth> so yeah it's not really worthwhile
22:29 -!- marainein [~marainein@114-198-65-190.dyn.iinet.net.au] has quit [Remote host closed the connection]
22:49 < kanzure> black ranger goes to college http://www.youtube.com/watch?v=hdMBEiOdMTI
23:03 < kanzure> how does hackteria.org have so many sponsors?
23:03 < kanzure> http://hackteria.org/wiki/index.php/HackteriaLab_2011_-_Romainmotier
23:09 < yashgaroth> europe, I guess
23:11 < yashgaroth> also how much sponsoring do they actually do? was there an attendance fee for that
23:13 < kanzure> dunno.
23:16 < kanzure> blah now the javascript problem isn't reproducible
23:40 < Stee|> woo
23:40 < Stee|> drunk.
23:40 < Stee|> happy 21st to me
23:46 < yashgaroth> congrats brah
23:48 < Stee|> yashgaroth, confirmed have female wingman for la jolla bar hopping
23:49 < yashgaroth> haha awesome
23:49 -!- ianmathwiz7 [~chatzilla@x-134-84-100-61.reshalls.umn.edu] has joined ##hplusroadmap
23:50 < Stee|> hey ian
23:51 < Stee|> I have augmented my natural human blood supply with alcohol
23:52 < rdb> ethanol is hardly a useful drug in my opinion
23:53 < ianmathwiz7> that's always nice
23:53 < yashgaroth> well you live in the land of drugs rdb
23:53 < rdb> I wish, we still have ridiculous drug laws
23:53 < rdb> just slightly less ridiculous than other places
23:54 < Stee|> 21st bday
23:54 < yashgaroth> oh hey I'm gonna be in the netherlands in july, can you recommend something fun outside of amsterdam?
23:55 < yashgaroth> I was thinking belgium but I hear it's boring
23:55 < JayDugger> Happy birthday!
23:55 < Stee|> yashgaroth: maybe I can try to be there too!
23:55 < Stee|> thanks JayDugger
23:56 < yashgaroth> hell yeah man, I've got an extra week to fuck around over there after I visit family
23:58 < Stee|> yashgaroth, I am....a bit wasted O_o
23:58 < yashgaroth> yeah I noticed a little
23:58 < rdb> happy birthday Stee|
23:59 < rdb> yashgaroth, nothing that compares to a trip to a smartshop or coffeeshop, no
23:59 < yashgaroth> aww damn oh well
23:59 < rdb> or to the red light district if you wish
--- Log closed Sat Feb 18 00:00:00 2012