--- Log opened Tue Mar 13 00:00:49 2012 00:07 -!- Mo|ybdenum [kvirc@173-30-241-198.client.mchsi.com] has quit [Quit: KVIrc 4.0.4 Insomnia http://www.kvirc.net/] 00:13 -!- Juul [~Juul@slim.dhcp.lbl.gov] has quit [Quit: Leaving] 00:24 -!- zacharycohn [~zacharyco@c-98-247-247-157.hsd1.wa.comcast.net] has quit [Quit: zacharycohn] 00:29 -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has joined ##hplusroadmap 00:29 -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has quit [Remote host closed the connection] 00:29 -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has joined ##hplusroadmap 00:31 -!- SolG [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap 00:34 -!- _sol_ [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 246 seconds] 00:36 -!- He||eshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has joined ##hplusroadmap 00:39 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has quit [Ping timeout: 244 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[Ping timeout: 248 seconds] 01:30 -!- Juul [~Juul@slim.dhcp.lbl.gov] has joined ##hplusroadmap 01:45 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 255 seconds] 01:49 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 01:54 -!- uniqanomaly [~ua@dynamic-78-8-84-170.ssp.dialog.net.pl] has joined ##hplusroadmap 01:55 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 02:42 -!- ybit [~ybit@unaffiliated/ybit] has quit [Ping timeout: 244 seconds] 02:47 -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has quit [Ping timeout: 244 seconds] 02:47 -!- gnusha_ [~gnusha@131.252.130.248] has quit [Ping timeout: 244 seconds] 02:47 -!- gnusha [~gnusha@131.252.130.248] has joined ##hplusroadmap 02:47 -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware http://gnusha.org/logs/ http://bit.ly/diybionews2 http://gadaprize.org/ http://groups.google.com/group/diybio 02:47 -!- Topic set by kanzure [~kanzure@131.252.130.248] [Mon Feb 13 12:33:19 2012] 02:47 [Users ##hplusroadmap] 02:47 [ _sol_ ] [ ferrouswheel ] [ nuba ] [ strangewarp] 02:47 [ archels ] [ gedankenstuecke] [ panax ] [ superkuh ] 02:47 [ audy ] [ gnusha ] [ ParahSailin ] [ sylph_mako ] 02:47 [ augur ] [ HEx1 ] [ pasky ] [ uniqanomaly] 02:47 [ bkero ] [ He||eshin ] [ poptire ] [ Urchin ] 02:47 [ CIA-92 ] [ ivan` ] [ qnm ] [ Utopiah_ ] 02:47 [ Coornail ] [ jennicide ] [ rdb ] [ Vicarious ] 02:47 [ d3nd3 ] [ jrayhawk ] [ rkos ] [ vrs ] 02:47 [ devrandom] [ Juul ] [ roksprok ] [ ybit ] 02:47 [ drazak ] [ kanzure ] [ saurik ] [ ziyadb ] 02:47 [ elmom ] [ katsmeow-afk ] [ splicer ] 02:47 [ epitron ] [ lichen ] [ Steel2 ] 02:47 [ fenn ] [ Mokbortolan_1 ] [ strages_home] 02:47 -!- Irssi: ##hplusroadmap: Total of 49 nicks [0 ops, 0 halfops, 0 voices, 49 normal] 02:47 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 02:47 -!- Irssi: Join to ##hplusroadmap was synced in 6 secs 02:49 -!- Netsplit *.net <-> 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[jen@173-19-241-225.client.mchsi.com] has quit [Read error: Connection reset by peer] 09:27 < ParahSailin> http://www.extremetech.com/extreme/122231-solar-panels-made-with-ion-cannon-are-cheap-enough-to-challenge-fossil-fuels this actually looks real 09:41 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Quit: Leaving.] 09:47 -!- zacharycohn [~zacharyco@216.190.29.118] has joined ##hplusroadmap 10:25 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 10:29 < archels> http://www.physorg.com/news/2012-03-extra-chromosomal-dna.html 10:52 -!- yottabit [~heath@unaffiliated/ybit] has joined ##hplusroadmap 10:53 < kanzure> Juul: low power ultrasound for brain stimulation http://diyhpl.us/~bryan/papers2/neuro/ultrasound/ 10:54 -!- lichen|2 [~lichen@c-76-105-164-184.hsd1.or.comcast.net] has joined ##hplusroadmap 10:56 -!- lichen [~lichen@c-76-105-164-184.hsd1.or.comcast.net] has quit [Ping timeout: 250 seconds] 10:56 -!- lichen|2 is now known as lichen 11:02 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has joined ##hplusroadmap 11:02 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Client Quit] 11:02 < kanzure> "Gram negative enteric bacteria Klebsiella pneumoniae and Escherichia coli has been increasingly common acquired ESBL enzymes that make them resistant to penicillins and cephalosporins." 11:02 < kanzure> "They are often multiresistant. Beta-lactam-type carbapenems has hitherto been the last line broad-spectrum drugs, but when the bacteria had acquired enzymes, known as ESBL-Carba, which also breaks down carbapenems, remains registered no antibiotic group with safe effect." 11:03 < kanzure> "In Sweden, ESBL-Carba still unusual. So far , 37 cases identified, almost exclusively asymptomatic cases related to healthcare abroad (Figure 1). This means that we still have no safe domestic spread of infection and that there is still opportunity to prevent this." 11:03 < kanzure> "Several reports describe deaths after severe infection with ESBL-Carba. In Paris, hit seven patients in a hospital, despite treatment died five. In Istanbul killed ten of fifteen patients. Five newly transplanted patients died in Essen, Germany. In Greece and Italy are between 15 and 50 percent of all Klebsiella pneumoniae from blood cultures resistant to carbapenems. Greece and India dominate the country of infection in the Swedish case." 11:04 < kanzure> 2007 paper http://soapimg.icecube.snowfall.se/strama/Strama%20ESBL%20eng.pdf 11:04 < kanzure> "swedish strategic programme against antibiotic resistance" is an awesome name 11:16 < Vicarious> hi 11:20 < kanzure> hello. 11:20 < Vicarious> how's life? 11:20 < kanzure> javascript 11:20 < kanzure> is not a way of life 11:23 < Vicarious> hacking is 11:23 < Mariu> it's the only way to fly 11:24 -!- augur [~augur@129.2.129.35] has quit [Remote host closed the connection] 11:24 < kanzure> Mariu: who are yu 11:24 < kanzure> you 11:25 < Mariu> some guy interested in Transhumanism 11:25 < Mariu> came across this channel in some Google searches 11:25 < Mariu> I live in Romania 11:26 < Mariu> and that's about it, I think 11:26 < kanzure> do you know any good romanian bulletproof hosting solutions? 11:26 < Mariu> no 11:35 < epitron> archels: i just read that microDNA article... 11:36 < epitron> wtf is this: "This result suggests that the DNA found in tissue cells may exhibit more variation than previously thought, and the implication of this is that sequencing of the DNA in blood cells (which are the cells usually used for sequencing) may give misleading results if microdeletions have occurred in the DNA of other tissues but not in blood cells." 11:36 < epitron> we only sequence blood cells?? 11:37 < epitron> how are blood cells the most representative cells in the body? :) 11:38 < epitron> http://en.wikipedia.org/wiki/Haematopoiesis 11:38 < epitron> look at this 11:39 < epitron> blood cells are weird, in that they're very special purpose 11:41 < kanzure> i'm p. sure we sequence all sorts of cells.. all the diybio stuff is cheek swabs 11:42 < epitron> aren't cheek swabs only really good for microarrays, because you get so much bateria in there too? 11:42 < epitron> or can you separate the skin cells by centrifuge? 11:44 < kanzure> you can separate dna by molecular weight and prolly centrifugation 11:44 < epitron> that still seems pretty messy though, for shotgun sequencing 11:44 < epitron> you won't get any of these circularDNAs from that method either 11:44 < kanzure> what are you trying to do? 11:45 < epitron> well, i'm not trying to do it, but i think science's goal is to get a representative sample of the population's DNA 11:46 < archels> I'm wondering if those circular DNA strands are pathological. 11:46 < epitron> didn't they discover recently that neurons strip out a bunch of their DNA to optimize the cell? 11:47 < epitron> like, after neurogenesis from stem cells 11:50 < epitron> i wouldn't be surprised if blood cells did the same thing 11:50 < epitron> since they don't really do much besides float around a while, then get broken down 11:50 < epitron> :) 11:54 < epitron> and the more real-estate you've got in a blood cell, the more oxygen-holding molecules you can cram in there 12:04 < ParahSailin> on sequencing blood cells: who knows, maybe they mean b cells immortalized by ebv 12:04 < ParahSailin> those sorts of cell lines are common in cell banks 12:04 < ParahSailin> because very easy to collect and then transform 12:10 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has quit [Ping timeout: 246 seconds] 12:28 -!- augur [~augur@129.2.129.35] has joined ##hplusroadmap 12:30 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 260 seconds] 12:41 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 12:49 -!- samaya [~samaya@194.150.85.181] has joined ##hplusroadmap 13:10 < kanzure> 'solid state civilization' http://www.aleph.se/andart/archives/2009/03/a_really_green_and_sustainable_humanity.html 13:10 < kanzure> samaya: hi 13:20 -!- yottabit [~heath@unaffiliated/ybit] has quit [Ping timeout: 246 seconds] 13:33 < uniqanomaly> next hitler will be greenie 13:35 < uniqanomaly> kill 'em all and copse forests 13:35 < ThomasEgi> eco-extremists? 13:36 < ThomasEgi> uniqanomaly, if you want forests, all you have to do is: nothing 13:36 < ThomasEgi> and few decades later. there will be forest all over where you did nothing 13:39 < uniqanomaly> http://innoplex.org/eng/2011/02/vertical-led/ that's awesum 13:43 < ThomasEgi> hm. that's a pretty awesome thing. will definetly get back to it when i finished my first food-lab in my underground city. 13:45 -!- poptire [~quassel@64.31.59.70] has quit [Quit: No Ping reply in 180 seconds.] 13:45 -!- Charlie [~quassel@64.31.59.70] has joined ##hplusroadmap 13:45 -!- Charlie is now known as Guest70952 13:49 -!- jmil [~jmil@2607:f470:8:3148:b9f4:e5d2:ffa3:5469] has joined ##hplusroadmap 13:54 < rkos> i thought the last hitler was a greenie too 14:14 -!- jmil [~jmil@2607:f470:8:3148:b9f4:e5d2:ffa3:5469] has quit [Quit: jmil] 14:33 -!- augur [~augur@129.2.129.35] has quit [Remote host closed the connection] 14:35 -!- augur [~augur@129.2.129.35] has joined ##hplusroadmap 14:37 -!- samaya [~samaya@194.150.85.181] has left ##hplusroadmap ["Leaving"] 15:00 -!- augur [~augur@129.2.129.35] has quit [Remote host closed the connection] 15:03 -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 15:06 -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has joined ##hplusroadmap 15:07 < fenn> wow E. O. Wilson is still alive? 15:08 < kanzure> termination was unsuccessful 15:08 < fenn> "E.O. Wilson will speak on his new book at the Exploratorium on April the 20th." 15:10 -!- augur [~augur@129.2.129.158] has joined ##hplusroadmap 15:11 -!- zacharycohn_ [~zacharyco@216.190.29.118] has joined ##hplusroadmap 15:12 -!- zacharycohn [~zacharyco@216.190.29.118] has quit [Ping timeout: 244 seconds] 15:12 -!- zacharycohn_ is now known as zacharycohn 15:15 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 248 seconds] 15:25 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 15:29 < kanzure> "The books were often a financial stretch, with many families paying for their encyclopedias in monthly installments." 15:43 -!- rkos [~chatzilla@a88-113-156-174.elisa-laajakaista.fi] has quit [Read error: Connection reset by peer] 15:43 -!- rkos [~chatzilla@a88-113-156-174.elisa-laajakaista.fi] has joined ##hplusroadmap 15:54 -!- ziyadb [u4806@gateway/web/irccloud.com/x-qdsdlqzrjtcdplwk] has quit [Ping timeout: 244 seconds] 15:58 -!- ParahSailin [~parah@unaffiliated/parahsailin] has quit [Remote host closed the connection] 16:04 -!- augur [~augur@129.2.129.158] has quit [Remote host closed the connection] 16:05 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 16:17 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 16:33 -!- ParahSailin [~parah@adsl-69-151-211-133.dsl.hstntx.swbell.net] has joined ##hplusroadmap 16:33 -!- ParahSailin [~parah@adsl-69-151-211-133.dsl.hstntx.swbell.net] has quit [Changing host] 16:33 -!- ParahSailin [~parah@unaffiliated/parahsailin] has joined ##hplusroadmap 16:48 -!- yottabit [~heath@unaffiliated/ybit] has joined ##hplusroadmap 16:50 < kanzure> http://finance.yahoo.com/news/assay-depot-biocurious-announce-open-140700957.html 16:50 < kanzure> "My name is Timothy Chen from Assay Depot. We have partnered with 16:50 < kanzure> BioCurious, a San Francisco-based laboratory hackerspace, to start up 16:50 < kanzure> a competition for Bay Area students and scientists. As a source of 16:50 < kanzure> hackerspace of your own, I thought that some LA Biohackers would be 16:50 < kanzure> interested as many people from SoCal have roots in the Bay Area and 16:50 < kanzure> may be interested. There is a $10,000 prize pool for research services 16:50 < kanzure> and full year memberships to the BioCurious laboratory hackerspace 16:50 < kanzure> available as prizes (which could be a great way for students to work 16:50 < kanzure> with other scientists). I did not want to email the Google Groups 16:50 < kanzure> mailing list just in case it was deemed inappropriate. 16:50 < kanzure> Our guidelines can be found at https://www.assaydepot.com/pages/challenge. 16:50 < kanzure> " 16:59 -!- augur [~augur@129.2.129.158] has joined ##hplusroadmap 17:05 < ParahSailin> the remainder of the prize money is earmarked for research services necessary to carry out the project whats this mean 17:10 -!- augur [~augur@129.2.129.158] has quit [Remote host closed the connection] 17:13 < kanzure> ParahSailin: that might mean the first $500 goes to the biocurious reagent store, or the second $500 has to go to assaydepot 17:14 < kanzure> i like the "money stuffed into a flask" image 17:14 < kanzure> i'm gonna steal that image. hell yeah 17:17 < ThomasEgi> all those "get 5000$ price"... i mean if you break that down to living costs and stuff. it is pretty much like asking people to invent something for almost-free. 17:18 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 17:19 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 252 seconds] 17:19 < kanzure> ThomasEgi: researchers tend to be poor and paid $10/hr 17:19 < Steel2> unless you're working for a corporation or the gov. 17:19 < kanzure> state universities still pay low amounts to researchers 17:20 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 17:20 < kanzure> defense contracting is a little different, but they still hire lab technicians at obscenely low prices 17:20 < kanzure> all those "associate degrees in lab tech" people 17:22 < Steel2> eh, I'd differentiate between researcher and lab tech. 17:22 < kanzure> Steel2: do you do any mems-related math on a regular basis 17:22 < Steel2> no, I don't do mems. I know some people who might tho 17:23 < Steel2> my lab doesn't do mems at all 17:23 < kanzure> huh? i thought you do some microjets 17:23 < kanzure> i'm writing some code to do mathy things for me regarding flow rates and pressures in channels 17:23 < Steel2> oh 17:23 < Steel2> microfluidics =/= mems 17:23 < kanzure> yes true 17:24 < Steel2> I can tell you about microfluidic impacts 17:24 < kanzure> how about the relation of pumping to flow rate to channel geometries 17:24 < kanzure> :x 17:24 < Steel2> oh christ. 17:24 < kanzure> i think it's probably simple 17:24 < Steel2> probably somewhere in my micromanufacturing notes 17:25 < Steel2> which I'm trying to get ahold of still 17:25 -!- zacharycohn [~zacharyco@216.190.29.118] has quit [Quit: zacharycohn] 17:27 < kanzure> it's probably something simple tho 17:28 < kanzure> like a "are you brain dead for even asking?" 17:28 < kanzure> http://en.wikibooks.org/wiki/Microfluidics/Hydraulic_resistance_and_capacity#Modelling_of_an_elastic_long_tube_with_a_substantial_pressure_drop 17:30 < kanzure> gee this is probably exactly the same as electronics 17:34 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 245 seconds] 17:35 -!- augur [~augur@129.2.129.35] has joined ##hplusroadmap 17:35 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has joined ##hplusroadmap 17:36 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 17:38 < kanzure> "Small ants can lift 1000 small ants. Note, that this force is used by a particular species of ants to jump. These ants strike their mandibles on the ground with a force that is 300 times its own weight, and propel themselves up to 10cm in height" 17:38 < kanzure> Patek, S. N. and Baio, J. E. and Fisher, B. L. and Suarez, A. V. (2006) Multifunctionality and mechanical origins: Ballistic jaw propulsion in trap-jaw ants, Proceedings of the National Academy of Sciences, vol 103, page 12787 17:38 < kanzure> http://www.pnas.org/cgi/content/abstract/103/34/12787 17:39 < kanzure> ballistic jaw propulsion 17:40 < kanzure> i wonder how it lands 17:41 < jrayhawk> If Morrowind has taught me anything, it has to land using the same technique 17:46 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 248 seconds] 17:48 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 17:52 < ybit> christof koch @singularity institute :: http://www.youtube.com/watch?v=5tWr6HW9xKw&feature=player_detailpage#t=76s 17:52 < ybit> first two mins watching if you don't watch anything els 17:52 < ybit> e 17:56 < kanzure> huh i forgot about how awesome mohorbiz is 17:56 < kanzure> http://diyhpl.us/~bryan/papers2/maharbiz/ 17:56 < kanzure> *maharbiz 17:56 < kanzure> he does some cyborg beetles and some microfluidics stuff 17:56 < kanzure> "A cyborg beetle - insect flight control through an implantable, tetherless microsystem" 17:56 -!- augur [~augur@129.2.129.35] has quit [Remote host closed the connection] 17:56 < kanzure> "Patterned delivery and expression of gene constructs into developing zebrafish embryos using microfabricated interfaces" 17:57 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 265 seconds] 17:59 < kanzure> huh surface tension actuated microfluidics.. http://diyhpl.us/~bryan/papers2/maharbiz/Transpiration%20actuation%20-%20the%20design,%20fabrication,%20and%20characterization%20of%20biomimetic%20microactuators%20driven%20by%20the%20surface%20tension%20of%20water.pdf 18:04 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 18:06 -!- marainein [~marainein@114-198-123-218.dyn.iinet.net.au] has quit [Ping timeout: 246 seconds] 18:10 -!- rkos [~chatzilla@a88-113-156-174.elisa-laajakaista.fi] has quit [Ping timeout: 244 seconds] 18:14 < kanzure> weird things i find in my collection, exhibit A: 18:14 < kanzure> http://diyhpl.us/~bryan/papers2/organic-tft/2010-02-01.txt 18:14 < kanzure> "I'm looking at amorphous and polycrystalline TFT based ICs that could conceivably support an X86 virtual mode compliant processor running at 50 - 100MHz. Running an old modified Slackware kernel, say. That's all you need if the library calls are made to ASICs made of the same stuff. For calls to matrix calculations; you can use a battery of specialized TFTs to do things really fast...." 18:14 < kanzure> "Bryan if this is irrelevant to u please delete real fast, and sorry!!" 18:15 < kanzure> why would anyone apologize to me for these things :x 18:15 < jrayhawk> did you rule with an iron fist 18:15 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 244 seconds] 18:17 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 18:18 < kanzure> gahhh 18:18 < kanzure> i forgot about /protein-purification .. it's now safely merged with /bio/protein-purification/ 18:20 < kanzure> delinquentme: you might enjoy http://diyhpl.us/~bryan/papers2/retrosynthesis/ 18:21 -!- yottabit [~heath@unaffiliated/ybit] has quit [Quit: Konversation terminated!] 18:21 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 255 seconds] 18:23 < kanzure> ""We assessed the forgetting of friends and its effects on measuring personal and social network characteristics and properties. All 217 residents of a university residence hall first recalled as many of their friends in the hall as they could." 18:23 < kanzure> "Then, on a complete list of hall residents, residents indicated other friends they forgot to recall." 18:23 < kanzure> "On average, residents forgot 20% of their friends. Residents' demographic characteristics are unrelated to the proportion of friends forgotten." 18:23 < kanzure> congratulations! i don't know 20% of you. 18:24 < kanzure> probably more. who the hell are you people? 18:25 < kanzure> http://diyhpl.us/~bryan/papers2/social/ 18:26 < kanzure> oh i forgot that dunbar estimated his number based on the size of the neocortex.. that's funky 18:35 * Vicarious arrived at home 18:36 -!- Yashgaroth [~fff@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 18:37 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 18:39 < Steel2> hey Yashgaroth, Daniel Finfer of Ancient Lasers/Posthuman era might go out with us when I finally make it to cali 18:39 < Yashgaroth> aw shit I better listen to his albums then 18:40 < Steel2> lol 18:40 < Yashgaroth> I mean, I've heard songs, but probably not the whole discography 18:40 < Steel2> whatever, it won't be for a while 18:40 < Yashgaroth> I thought you were coming next month 18:41 < kanzure> Yashgaroth: i misplaced some files and you might enjoy the updates to http://diyhpl.us/~bryan/papers2/bio/protein-purification/ 18:42 < Yashgaroth> sweet 18:42 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 18:42 < Yashgaroth> also re: epitron earlier, genome sequencing of 'blood cells' is of immune cells; red blood cells don't have a nucleus 18:44 < Juul> blood isn't alive! 18:44 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 240 seconds] 18:45 < Yashgaroth> billions of zombies, floating around inside you right now 18:45 < kanzure> only billions? 18:45 < kanzure> "There are about 30 trillion red blood cells in the average human body." 18:45 < kanzure> 30 trillion zombies is way different man 18:45 < Steel2> Yashgaroth: I was going to, thesis advisors are being dicks. 18:46 < Steel2> so my dad's shipping the car instead of me flying out and driving it back 18:46 < Juul> https://inspirescience.files.wordpress.com/2010/11/carl_sagan2.jpg 18:46 < kanzure> Juul: i finally watched "cosmos" a few weeks ago.. 18:46 < Yashgaroth> well damn...I might be visiting NY/DC at some point in may-ish 18:46 < Steel2> cool 18:46 < Juul> kanzure, oh yeah? i've only watched clips. just how outdated does it feel? 18:46 < kanzure> the romantizing of science was really not cool 18:46 < Steel2> I'll still be in SD a decent amount for work or visiting dad 18:47 < Yashgaroth> well, I'll be here for a while, hopefully 18:47 < epitron> Yashgaroth: ah, i see.. that makes more sense... 18:47 < Juul> kanzure, hm, now i have to watch it 18:47 < kanzure> Juul: no it's not too outdated ... for the astrophysics 18:47 < kanzure> damn i should have kept a list of things 18:48 < Yashgaroth> also kanzure, where did you get the 30 trillion number? I'm p. sure the whole body is only ~100 trillion 18:48 < kanzure> but anyway, if you can get past all the "i want to touch you while we talk about science" stuff it's ok 18:48 < Yashgaroth> oh it's wikipedia 18:48 < Juul> kanzure, i'll probably like it. i like sagan's writing. 18:49 < kanzure> i like sagan's concepts but i really dislike how he makes a nebula some romantic thing instead of a cloud of dust 18:50 < Steel2> the point is to show people that reality is just as beautiful as mysticism 18:50 < Yashgaroth> I've got a mental image of sagan making out with a galaxy now 18:50 < Juul> you don't like the mixing of poetry and sicence 18:50 < Juul> i can see that 18:50 < kanzure> poetry has its place 18:50 < kanzure> i am ok with poetry in general 18:50 < kanzure> i am also ok with science-related poetry 18:51 < kanzure> at least feynman admitted it was the pleasure of finding things out, and not some sort of inherent pleasure that electrons possess 18:51 < Juul> yeah i do think he sometimes goes a bit overboard with the poetic imagery when the facts are actually more awe-inspiring 18:52 -!- zacharycohn [~zacharyco@c-98-247-247-157.hsd1.wa.comcast.net] has joined ##hplusroadmap 18:59 -!- zacharycohn [~zacharyco@c-98-247-247-157.hsd1.wa.comcast.net] has quit [Quit: zacharycohn] 19:01 -!- Juul [~Juul@slim.dhcp.lbl.gov] has quit [Quit: Leaving] 19:01 < kanzure> http://www.genomeweb.com/coalition-wants-moratorium-extreme-synthetic-bio-businesses 19:01 < kanzure> " An international advocacy coalition today 19:01 < kanzure> called for a moratorium on the development of new synthetic organisms 19:01 < kanzure> for commercial use while new international regulations for governing 19:01 < kanzure> the synthetic biology sector are created to protect the environment 19:01 < kanzure> and people from unknown perils." 19:01 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 19:01 < kanzure> what the fuck is a "global moratorium" and why would they think i would obey it? 19:02 < kanzure> "Friends of the Earth and over 100 international groups focused on 19:02 < kanzure> environmental, bioscience, food safety, human and consumer rights 19:02 < kanzure> issues, and religion, said in a report published today that although 19:02 < kanzure> the synbio market had a value of more than $1.6 billion in 2011 and 19:02 < kanzure> could hit $10.8 billion by 2016, there has been "little or no 19:02 < kanzure> governance of the industry or assessment of the novel risks posed by 19:02 < kanzure> synthetic organisms." 19:02 < kanzure> bleh 19:03 < kanzure> "The group also wants mandatory regulations that would treat synthetic 19:03 < kanzure> biology as a unique activity and would be stronger than current the 19:03 < kanzure> regulations on pathogens, containment, drugs, and worker protections." 19:03 < Yashgaroth> pfft they're not even biologists 19:03 < kanzure> "Among other proposals, the coalition also said it wants a prohibition 19:03 < kanzure> on the use of synthetic biology to change the human genetic makeup, 19:03 < kanzure> human genome, epigenome, or microbiome, because any such genetic 19:03 < kanzure> alterations "are too risky and fraught with ethical concerns."" 19:03 < kanzure> Yashgaroth: the majority of people who make laws are also not biologists 19:04 -!- Steel2 [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Read error: Connection reset by peer] 19:04 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap 19:06 < Yashgaroth> but look at 'their staff': attorney/activist/author, a methodist and 'fellow' at some other bogus institute, another attorney, '18 years experience working in office management', BA in anthro, journalist 19:07 < Yashgaroth> "The United Methodist Church’s staff director of genetics" 19:07 < Stee|> heh 19:07 < Yashgaroth> the anthro major is their "research support" 19:08 < kanzure> unfortunately there's not that many orgs that can argue against things like this 19:08 < Stee|> kanzure: You don't need much 19:08 < kanzure> when a govt body receives a demand letter like that signed with 100 orgs they look at it pretty closely 19:08 < Stee|> 500 bucks for an LLC and all the paperwork 19:08 < kanzure> yes but you need 100+ of these 19:08 < kanzure> let's see.. what do we have? 19:08 < Yashgaroth> until they ask the AAAS about it and they go "haaaaahahaha" 19:08 < Stee|> how many synthetic bio advocacy groups are there? or do we even need to bother, considering pharma companies 19:08 < kanzure> AAAS would probably join that list, Yashgaroth 19:09 < kanzure> well no i mean non-profits 19:09 < kanzure> because if it's just industry vs. non-profits, the non-profits have an easy argument ("they are all afraid of regulation and taxes") 19:09 < Stee|> welp, time to start starting up those pro-h+ nonprofits 19:09 < kanzure> no... that's not what i asked :( 19:09 < Yashgaroth> we have no reputable h+ nonprofits worth a shit 19:09 < kanzure> and the 'time' to do that is not at all influenced by whether or not someone sends a giant letter 19:09 < kanzure> they send these all the time 19:10 < Stee|> eh, the time to do it is asap 19:10 < Stee|> yashgaroth: yet 19:10 < Yashgaroth> true 19:10 < kanzure> and whether or not to start one has nothing to do with whethero r not thse other groups are sending letters 19:10 < kanzure> i find your logic to be full of shit 19:10 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 265 seconds] 19:10 < Stee|> Non profits look good from a pr pov 19:10 < Stee|> to normal people 19:11 < kanzure> anyway, i specifically asked for a list of synthetic biology orgs 19:11 < Stee|> oh 19:11 < Stee|> no idea 19:11 < kanzure> "let's see... what do we have?" 19:11 < kanzure> well there's openwetware, biofab, synbioberc 19:11 < kanzure> biobricks foundation 19:11 < kanzure> jcvi 19:11 < Yashgaroth> there weren't even any orgs for genetic engineering, back before it got renamed synbio 19:11 < kanzure> although, i think synbioberc is probably listed on that antisynbio letter 19:12 < kanzure> Yashgaroth: iirc there's a lot of microbiology genetics orgs that are quasi related to AAAS 19:12 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 19:12 < Yashgaroth> well sure, but not explicitly synbio-oriented 19:13 < Yashgaroth> not that I really keep track of these things 19:13 < kanzure> Stee|: why would your humanity+ be better than regular humanity+ 19:13 < Stee|> I didn't say I wanted to start a H+ equivalent (yet) 19:14 < kanzure> 19:09 < Stee|> welp, time to start starting up those pro-h+ nonprofits 19:14 < Stee|> pro transhumanism 19:14 < kanzure> so is humanity+ 19:14 < kanzure> .... theoretically. 19:14 < Stee|> yeah, but all I want to start with is provide an umbrella for people to order various lab supplies and shit, and give support to, without looking like terrorists 19:15 < kanzure> i thought you wanted PR 19:15 < Stee|> we'll see where it goes after that 19:15 < kanzure> make up your mind :( 19:15 < Stee|> that's part of PR? 19:15 < kanzure> um.. not really 19:15 < Stee|> it's all intertwined 19:15 < kanzure> that sounds like lab supply distribution 19:15 < kanzure> intertwined is not a good way to describe what you're doing 19:16 < Stee|> I need to build up reputation in the community, outside the community, with people peripherally related to the community, etc. 19:16 < Stee|> also building up organizational reputation 19:16 < Stee|> these are all different efforts 19:16 < kanzure> meh 19:16 < Stee|> that culminate with the same goals 19:16 < kanzure> ok what does that have to do with lab supply distribution? 19:17 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 244 seconds] 19:17 < Stee|> by making people's lives easier with various supplies, I get rid of one annoyance/obstacle 19:17 < Stee|> as well as possibly helping with some sort of standardization/discount 19:17 < kanzure> obstacle to reputation? 19:17 < Stee|> obstacle to their own research 19:17 < kanzure> ok so again i don't see why reputation matters there 19:17 < kanzure> just provide a good service and reputation will happen 19:17 < kanzure> and/or don't care about it 19:18 < Stee|> that's exactly what I said basically 19:18 < kanzure> no i know you better than that 19:18 < Stee|> by helping out I gain reputation AND advance the science 19:18 < kanzure> you've told me on other occassions that you'd rather be doing ads, publicity, PR, PR stunts, lobbying, etc. 19:19 < kanzure> and "social forums" 19:21 -!- Stieru_Ridir [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap 19:21 -!- Stieru_Ridir is now known as Steel2 19:21 < Steel2> router issues, last thing I saw was [Stee|] by helping out I gain reputation AND advance the science 19:22 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Ping timeout: 252 seconds] 19:27 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 19:33 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 255 seconds] 19:37 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 19:55 -!- zacharycohn [~zacharyco@c-98-247-247-157.hsd1.wa.comcast.net] has joined ##hplusroadmap 20:10 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 248 seconds] 20:10 < Mariu> see you guys 20:12 -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap 20:12 -!- Mariu [Jimmy98@89.41.57.33] has quit [Quit: leaving] 20:35 -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 248 seconds] 20:50 -!- klafka1 [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 21:45 < kanzure> economics of anime http://www.animenewsnetwork.com/feature/2012-03-07 21:52 < kanzure> "This change in consumer behavior hit anime particularly hard, since during the industry's heyday, it depended on people collecting multiple volumes of a show they'd never seen. The idea of expecting people to commit up to $200 to collect a show they might not even like, in retrospect, seems like insanity." 21:52 < kanzure> what? 21:56 < Steel2> oh, yeah 21:56 < Steel2> they'd do these ultra niche fanservice shows to hit one particular market really hard 21:56 < Steel2> and make up for it with massive prices 21:57 < katsmeow-afk> haute couture in movies 21:57 < kanzure> "It's been slow and painful. Licensors were reluctant to give up the safety of videotape, which was secure, long-lasting and reliable, and while it cost quite a bit of money, was uncomplicated and easy to work with." 21:57 < kanzure> "It wasn't until the 2011 Tohoku Earthquake and tsunami wiped out Sony's only broadcast tape manufacturing plant (and the worldwide tape shortage that followed) that many of the bigger licensors started taking a serious look at going "tapeless"." 21:59 < kanzure> "How much money? Most anime these days is mastered on a tape format called HD-CAM. The price of a single recorder starts at US$45,000." 21:59 < kanzure> .. but i bet it costs way less than $5k in parts 21:59 < lichen> thats how it goes 21:59 < lichen> but you could go into the economics of high end technical equipment too 22:00 < lichen> the amount of development and testing required costs money 22:00 < lichen> which means prices are going to be artifically inflated for a while 22:01 < strangewarp> ... Friends of the Earth, almost right down to their organization's name, are nearly identical in composition, spirit, and goal to a fictional organization on a roleplaying game I used to be active in. Ultimate weirdness. 22:05 < kanzure> strangewarp: the game was probably based around these real-life orgs 22:06 < kanzure> "So overall, over 22 full length episode series and so around 90 episodes a month, the funds from Crunchyroll would cover the cost of about one episode ~ if the streaming process itself was cost free, which it is not." 22:07 < lichen> the media industry is all kinds of broken right now 22:15 < kanzure> relying on advertising revenue is kind of silly 22:15 < kanzure> "we can't figure out how to make money from this" 22:15 < kanzure> "oh i know! we can get money from ads, they'll know how to make money from this!" 22:15 < kanzure> but in reality ads don't really pay out that much 22:15 < lichen> yep 22:15 < kanzure> because advertising doesn't work 22:15 < kanzure> unless you're building brand awareness 22:15 < lichen> and when all else fails, lobby your way into money 22:16 < lichen> if people dont want to pay you, just sue them untilt hey have to 22:16 < kanzure> no that doesn't work either 22:16 < lichen> im aware 22:16 < lichen> sarcasm 22:16 < kanzure> i'm a little surprised that people think ads are a viable solution 22:17 < lichen> well as long as corpos keep feeding them money for that 22:17 < lichen> it is 22:17 < lichen> mostly just blame corps for putting so much of their operating budget into advertising 22:17 < kanzure> the reason ad agencies used to make so much money was because nobody could measure their performance 22:17 < kanzure> $20mil or $50mil to an ad agency is very commonly not measured 22:17 < lichen> ah 22:18 < kanzure> "oh look! we advertised during a tv show with 4 million viewers (maybe)" 22:18 < kanzure> except you charged $3mil to do that ad, and only 100 people want to buy it 22:18 * katsmeow-afk points to a case where $1500 in "buy one 2nd is half off (if you bring in this coupon))" got zero results,, so in that case advertising was worthless 22:18 < strangewarp> kanzure: I dunno, the game had been running since the 1990s. But whatever [late reply; was doing things] 22:19 < kanzure> strangewarp: yeh these orgs have been around for a while 22:19 < kanzure> i think 'friends of earth' or that one about farmers has been around for decades now 22:19 < katsmeow-afk> Mother Earth News 22:19 < strangewarp> Oh, hmm. Might have been the original inspiration, then.. 22:20 < katsmeow-afk> there was another that began alongside of it that got big too 22:20 < katsmeow-afk> FOE was into ecoactivism/terrorism, MEN wasn't 22:20 < lichen> lol, MEN 22:20 < katsmeow-afk> iirc 22:20 < lichen> pretty bad acronym for that 22:21 < katsmeow-afk> it's after midnite, by brain is off 22:21 < katsmeow-afk> yeas 22:21 < katsmeow-afk> go into store, ask for MEN 22:22 < strangewarp> Get entirely the wrong magazine :p 22:22 < lichen> lol 22:27 < Steel2> Hmmm. 22:27 < Steel2> Yashgaroth, you around? 22:27 < Yashgaroth> ! 22:28 < Steel2> Should I try to make an H+ house in north county when my dad moves back east and we're left with an empty 3600 square foot house in vista? 22:28 < Steel2> :V 22:28 < Yashgaroth> how's the commute 22:29 < Steel2> eh 22:29 < Steel2> 30 mins to LJ 22:29 < Steel2> probably 22:30 < katsmeow-afk> La Jolla? 22:30 < Yashgaroth> yes 22:30 < Yashgaroth> I can stop by on weekends but I ain't living there, it's further than carlsbad 22:31 < Steel2> yeah, it's 37 miles 22:31 < Steel2> I won't be down there but my mom might be. Maybe just throw get togethers when I'm in state 22:32 < Yashgaroth> sure then 22:32 < Steel2> or drive up to LA 22:44 < Yashgaroth> something particular in LA? 22:44 < Steel2> some more buddies, better bars 22:44 < Steel2> it depends what we can find that's central to everyone 22:45 < lichen> heh, h+ house? 22:45 < kanzure> can you dorks do me a huge favor? go do a google query and count the number of seconds for the page to load 22:45 < lichen> google's gotten pretty obnoxious lately 22:45 < kanzure> also paste the number that google claims it took (for me it's roughly 0.3 seconds on average) 22:45 < Yashgaroth> 0.11 seconds 22:45 < lichen> what search 22:45 < lichen> pick a term 22:46 < katsmeow-afk> so use duckduckgo 22:46 < lichen> varying from .1-.2 22:46 < lichen> yeah, thats what i do already 22:46 < lichen> heh 22:46 < katsmeow-afk> google is doing tracking something fiercenow 22:51 < Steel2> I really don't like new google 22:52 < Steel2> *the browser 22:52 < Steel2> err 22:52 < Steel2> search engine 22:52 < Steel2> herp 22:52 < katsmeow-afk> chrome? 22:52 < katsmeow-afk> o 22:54 -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has joined ##hplusroadmap 22:54 -!- Mokbortolan_ [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 22:56 < kanzure> Yashgaroth: yes but how many seconds did you count 22:56 < Yashgaroth> half of one 22:57 < kanzure> it looks like google+ is the culprit 22:57 < kanzure> without cookies or being logged in the load times are more sane/reasonable 22:57 < kanzure> i wonder if the search page load time increases geometrically with the number of connections i have in google+ 22:58 -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Ping timeout: 244 seconds] 22:59 < katsmeow-afk> i would rather not google picked search results based on my previous "viewing habits", and instead gave me the best search results that match the terms i supply 23:00 < lichen> yeah 23:00 < katsmeow-afk> when i search for "spoke" (past tense of speak) today, and search for "spoke" (wheel component) tomorrow, i do not want that taken into consideration in searching tomorrow 23:00 < lichen> this is why i block all of google's shit 23:00 < lichen> they do some things that in some lights sound like they could be a good idea 23:01 < lichen> but in practice are terrribleeee 23:03 < katsmeow-afk> gnites 23:11 -!- ziyadb [u4806@gateway/web/irccloud.com/x-mbqprbffhuiohdhy] has joined ##hplusroadmap 23:11 -!- kvltist [~Kvltist@p5B33EADE.dip.t-dialin.net] has quit [Ping timeout: 265 seconds] 23:17 < fenn> under 3 seconds logged in w/plus 23:18 -!- ParahSailin [~parah@unaffiliated/parahsailin] has quit [Ping timeout: 260 seconds] 23:23 < kanzure> fenn: you're cheating.. you guys pay for like a direct line to google 23:30 -!- Juul [~Juul@208.87.217.74] has joined ##hplusroadmap 23:36 < klafka1> really? 23:37 -!- uniqanomaly_ [~ua@dynamic-78-8-93-128.ssp.dialog.net.pl] has joined ##hplusroadmap 23:37 -!- uniqanomaly [~ua@dynamic-78-8-84-170.ssp.dialog.net.pl] has quit [Ping timeout: 252 seconds] 23:46 < kanzure> klafka1: yeh one of them works at google 23:46 < kanzure> and puts the connection on the company's dime 23:49 < klafka1> aah 23:50 < klafka1> oh yeah i hear they do that --- Log closed Wed Mar 14 00:00:50 2012