--- Log opened Wed Mar 28 00:00:10 2012 00:04 -!- Steel2 [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Read error: Connection reset by peer] 00:40 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap 00:46 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Quit: Leaving] 02:41 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 02:51 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 240 seconds] 03:17 -!- ThomasEgi [~thomas@pppdyn-2d.stud-ko.rz-online.net] has joined ##hplusroadmap 03:17 -!- ThomasEgi [~thomas@pppdyn-2d.stud-ko.rz-online.net] has quit [Changing host] 03:17 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 03:20 -!- sylph_mako [~mako@118-92-239-9.dsl.dyn.ihug.co.nz] has quit [Ping timeout: 252 seconds] 03:20 < Vicarious> 'morning 03:23 < ThomasEgi> mornin 04:50 -!- thesnark [~thesnark@unaffiliated/thesnark] has joined ##hplusroadmap 05:20 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 05:24 -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection] 05:36 -!- kvltist [~Kvltist@p5B33F40D.dip.t-dialin.net] has joined ##hplusroadmap 06:14 -!- augur [~augur@206.196.184.189] has joined ##hplusroadmap 06:26 -!- n_bentha [~lolicon@75.111.160.104] has left ##hplusroadmap [] 06:49 -!- ThomasEgi [~thomas@pppdyn-2d.stud-ko.rz-online.net] has joined ##hplusroadmap 06:49 -!- ThomasEgi [~thomas@pppdyn-2d.stud-ko.rz-online.net] has quit [Changing host] 06:49 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 07:19 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Ping timeout: 265 seconds] 07:21 -!- n_bentha [~lolicon@75.111.160.104] has joined ##hplusroadmap 07:23 -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap 07:31 -!- ThomasEgi [~thomas@pppdyn-0f.stud-ko.rz-online.net] has joined ##hplusroadmap 07:31 -!- ThomasEgi [~thomas@pppdyn-0f.stud-ko.rz-online.net] has quit [Changing host] 07:31 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 07:38 -!- cluckj [~cluckj@cpe-67-246-45-105.nycap.res.rr.com] has joined ##hplusroadmap 07:47 < cluckj> kanzure do you know anything about singularity U's synbio launchpad thing? 08:00 -!- jmil [~jmil@2607:f470:8:3148:7013:2583:791f:49c7] has joined ##hplusroadmap 08:40 < audy> People are 3D printed by their mothers 08:43 < Mokbortolan_1> uhh 08:43 < Mokbortolan_1> no 08:43 < Mokbortolan_1> no they're not 08:43 * Mokbortolan_1 spergs out. 08:45 < archels> self-assembly, more like. 08:45 -!- AdrianG [~amphetami@unaffiliated/amphetamine] has joined ##hplusroadmap 08:52 -!- augur [~augur@206.196.184.189] has quit [Remote host closed the connection] 08:53 -!- augur [~augur@206.196.184.189] has joined ##hplusroadmap 08:54 < ThomasEgi> modern dental tech is quite something..that broken off tooth of mine now looks better than it did befor it snapped 08:56 -!- He||eshin is now known as Helleshin 08:57 -!- augur [~augur@206.196.184.189] has quit [Ping timeout: 246 seconds] 09:16 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Ping timeout: 260 seconds] 09:17 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap 09:28 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has quit [Read error: Connection reset by peer] 09:29 -!- augur [~augur@206.196.184.189] has joined ##hplusroadmap 09:31 -!- Stee| [~Steel@cpe-67-246-36-165.nycap.res.rr.com] has joined ##hplusroadmap 09:53 -!- eudoxia [~eudoxia@r190-135-60-50.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 09:58 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has joined ##hplusroadmap 10:01 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has quit [Ping timeout: 252 seconds] 10:13 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Quit: Leaving.] 10:14 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has joined ##hplusroadmap 10:15 < Urchin> lol, I don't need much dentistry done, so I freaked out my new dentist first time I had to get some drilling done by requesting that she does that without anesthetics 10:16 < n_bentha> masochist much? 10:17 < kanzure> n_bentha: nope, just thoughtless :P 10:17 < Urchin> lol, no 10:18 < Urchin> it was a minor repair 10:18 < Urchin> the anesthetic would be more trouble than it was worth 10:18 < n_bentha> Ah. I see. 10:19 < Urchin> I usually go to the dentist couple of times in a row every 5 or 6 years 10:19 < Urchin> last time I went anesthetic injections were not given away for everything 10:20 < Urchin> *before that time 10:21 < Stee|> anaesthesia doesn't work on me, or at least the last one they used didn't 10:21 < Stee|> so they used 12 shots of novacaine instead 10:22 -!- delinquentme [~asdfasdf@c-24-131-65-213.hsd1.pa.comcast.net] has joined ##hplusroadmap 10:22 < n_bentha> I'm really sensitive to anesthetics. 10:22 < n_bentha> Alcohol is usually good enough for me 10:27 < kanzure> n_bentha: but really. how did those transformations go? 10:27 < kanzure> cluckj: yes i do 10:28 < n_bentha> T_T 10:28 * n_bentha cries 10:28 < n_bentha> So they took up the original plasmid just fine. Had about 100 colonies on the kanamycin plate. 10:29 < kanzure> did yashgaroth murder your culture 10:29 < kanzure> ah 10:29 < n_bentha> But the plasmid that I inserted my gene fragment into...none of the bacteria expressed resistance to kanamycin, and the plates were empty :O 10:29 < n_bentha> Yeah, I think yashgaroth murdered them 10:31 < audy> your kanamycin might've been super-kanamycin by accident 10:31 < audy> did you grow them on non-kan plates as well? 10:34 < cluckj> anything interesting, kanzure? 10:34 < kanzure> cluckj: sure i have things to say about them.. 10:35 < cluckj> haha 10:35 < kanzure> i just woke up, can i rant at you later about them? 10:35 < cluckj> yup 10:35 < kanzure> i think the program is generally good but there's a few weird choices 10:35 < kanzure> like, for instance, why in holy hell is eri gentry an advisor 10:35 < kanzure> eri's startups haven't done much 10:40 < cluckj> isn't she in charge of biocurious? 10:41 < n_bentha> audy... 10:42 < audy> n_bentha yep? 10:42 < n_bentha> they grew on the kana plates just fine w/ the original plasmid...but not the plasmid that i inserted a gene fragment into 10:42 < audy> which plasmid? 10:43 -!- eudoxia [~eudoxia@r190-135-60-50.dialup.adsl.anteldata.net.uy] has quit [Remote host closed the connection] 10:43 < n_bentha> one w/ a chemical inducible promote 10:44 < audy> n_bentha E. coli? 10:44 < n_bentha> Yup 10:45 < audy> n_bentha do you have a link to the promoter spec? 10:50 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Quit: Leaving.] 10:52 < n_bentha> sorry, audy. i don't have one at the moment. 10:53 < audy> n_bentha: It'd be weird but is the insert site in the kan-resistance gene? 10:53 < n_bentha> I sure hope not! 10:54 < n_bentha> I thought that might be the case. I wanted to try them w/ another antibiotic plate as well. 10:56 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has joined ##hplusroadmap 11:14 -!- sylph_mako [~mako@118-92-239-9.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap 11:22 -!- Mariu [Jimmy98@89.41.57.33] has quit [Ping timeout: 265 seconds] 11:24 -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap 11:24 < Mokbortolan_1> http://www.process.org/discept/2011/11/17/draco-death-to-the-virus/ 11:25 < Mokbortolan_1> Article about MIT research that could potentially spell the end of viral infections 11:25 < kanzure> 1) that sounds a lot like hype to me 11:26 < Mokbortolan_1> claims, evidence, all that 11:26 < Mokbortolan_1>  DRACO proved successful against all 15 viruses tested “including rhinoviruses that cause the common cold, H1N1 influenza, a stomach virus, a polio virus, dengue fever and several other types of hemorrhagic fever.” [2] 11:26 < kanzure> oh, a caspase rna oligo 11:26 < kanzure> hrmm 11:27 < Mokbortolan_1> right now it's produced in modified bacteria 11:28 < n_bentha> thank for the 2011 article. this is 2012 btw 11:28 < Mokbortolan_1> uhh 11:28 < Mokbortolan_1> Nov. 2011 11:28 < Mokbortolan_1> six months ago 11:29 < n_bentha> Then how come I heard about it in august? 11:29 < Mokbortolan_1> 'cos the paper itself came out last July 11:29 < kanzure> what? 11:29 < n_bentha> Yea, so not 6 months ago. 11:29 < Mokbortolan_1> the article was 6mo ago 11:30 < Mokbortolan_1> so, would you prefer that I only talk about papers submitted in the last four months? 11:30 < Mokbortolan_1> err, published 11:31 < n_bentha> that article and the paper it refers to were published at different times? 11:31 < Mokbortolan_1> yes 11:31 < n_bentha> nevermind, i'm going to go yell at some undergrad. 11:31 -!- n_bentha [~lolicon@75.111.160.104] has quit [Quit: n_bentha] 11:31 < kanzure> nice 11:31 < kanzure> n_bentha is legit 11:33 < Mokbortolan_1> I was going to suggest he put me on ignore to prevent the offense of his sensibilities in the future 11:33 < kanzure> no i think he misinterpreted something 11:33 < kanzure> /or/ one of his undergrads lied to him 11:35 < Mokbortolan_1> seems like pretty exciting research 11:35 < Mokbortolan_1> no idea why AIDS funders aren't all over this, or maybe they've been burned too many times in the past 11:39 < kanzure> non-profits don't operate efficiently 11:39 < Mokbortolan_1> ohhhh 11:39 < kanzure> they just have people send in grant proposals, and they may or may not choose it based on your pedigree or something 11:39 < Mokbortolan_1> I know why... this was the plot line from "I Am Legend" 11:41 < Mokbortolan_1> interesting comment on reddit, what would it do to people with inactive herpes infections? 11:47 < Mokbortolan_1> it would also completely stop this: http://www.panspermia.org/virus.htm 11:50 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 11:51 < kanzure> yashgaroth: his colonies diiied 11:52 < kanzure> he is suing you for the damages 11:52 < yashgaroth> them dying implies that they grew first 11:52 < kanzure> uh 11:52 < kanzure> uhh 11:52 < yashgaroth> wait lemme read the logs 11:52 < kanzure> no, they could survie without resistance, but they would be killed off soon 11:53 < kanzure> *survive 11:53 < Mokbortolan_1> maybe that's what he was grumpy about 11:54 < yashgaroth> oh it looks like the fragment insertion fucked up the resistance gene somehow 11:54 < yashgaroth> at least his transformation of the original plasmid worked, which I imagine wasn't happening before, so I'm vindicated 11:55 < kanzure> haha 11:58 < katsmeow-afk> Mokbortolan , they aren't all over it because they make far more money selling lots of medicines that marginally might work, which you must keep on buying 11:58 < yashgaroth> DRACO won't work for HIV, or any other virus with latency 11:59 < katsmeow-afk> even if given continuously? 11:59 < yashgaroth> you can probably get it below detectable levels, but it'd be far more expensive than the current small-molecule treatments 12:00 < katsmeow-afk> why more expensive? they'll make the stuff in huge vats of bacteria 12:00 < yashgaroth> GMP protocols for biologics is far more strenuous than for small-molecule 12:01 < yashgaroth> the current HIV drugs are only expensive because they're recouping research costs 12:01 < kanzure> and because you pay for them 12:01 < yashgaroth> and because americans will pay a shit-ton for them, yes 12:02 < kanzure> in cases other than STDs, for instance in rare diseases, there are many "cures" in the patent database, but there's all sorts of licensing costs if you want to sell it 12:02 < kanzure> in many cases, you could just manufacture it yourself 12:02 < kanzure> for much less. 12:02 < yashgaroth> Myriad's BRCA test being the most obvious one 12:02 < kanzure> actually, i haven't done a full review of this. i should probably publish instructions for some common things. 12:02 < kanzure> yes, true 12:03 < kanzure> but for instance: crohn's. 12:03 < kanzure> there should probably be a site about this.. sometimes the rare diseases groups are too small to make a profit, but they are definitely large enough to support themselves with DIY methods 12:04 < yashgaroth> sometimes the rare diseases can be the most profitable, e.g. Alexion 12:05 < yashgaroth> $2mil per patient per year, with only a few dozen cases 12:05 < kanzure> yeah, so, $2mil is more than enough to fund DIY stuff 12:05 < kanzure> obv. patients get financial assistance for that, but there are some that will afford it 12:06 < kanzure> there are a few blood diseases that cost >$1,000/week.. that would definitely be able to support a DIY ecosystem 12:06 < yashgaroth> all the hemophilias 12:06 < kanzure> i'm not familiar with them, really 12:07 < yashgaroth> they're all hella expensive, from what I hear 12:07 < kanzure> yes 12:07 < kanzure> http://blogs.nature.com/spoonful/2011/09/soliris.html 12:07 < kanzure> "you can expect to shell out more than $400,000 per year because that’s the price of the antibody drug that just received regulatory approval in the US to treat the clotting disease." 12:07 < kanzure> ok.. $400k/year for an antibody? hahah 12:07 < kanzure> an antibody project is totally doable 12:08 < kanzure> "Atypical hemolytic-uremic syndrome (aHUS) is a life-threatening genetic disease affecting fewer than 1,000 Americans in which red blood cells break apart as they squeeze through small blood vessels leading to anemia, abnormal bleeding and kidney failure." 12:08 < kanzure> " (The monoclonal antibody, which is directed against the complement protein C5, had been on the market since 2007 for the treatment of another rare blood disorder called paroxysmal nocturnal hemoglobinuria.)" 12:08 < yashgaroth> antibodies cost maybe $1/mg to manufacture if you don't have to recoup clinical trial and research costs 12:08 < kanzure> i don't expect antibody manufacturing facilities to cost more than.. $30-$50k 12:08 < kanzure> especially in low volume 12:09 < yashgaroth> well...with a nice stable cell line, you can get 5 grams per liter of culture 12:09 < kanzure> by low volume i meant 1 person 12:09 < yashgaroth> one liter of media costs 50 bucks 12:09 < kanzure> but 1000 patients is already low volume :) 12:10 < kanzure> well let's see how many grams in a dose 12:10 < kanzure> 600-900 mg per week 12:10 < kanzure> oh, 600-900 mg per week for the first few weeks, followed by 300-400 mg per week 12:11 < yashgaroth> 10 mg/kg body weight, every 2 weeks, is a very rough average; it depends on the disease 12:12 < kanzure> yeah, um. 12:12 < yashgaroth> actually I'd say $0.1/mg is doable 12:13 < yashgaroth> *with enough seed money to develop the stable cell line 12:13 < kanzure> http://en.wikipedia.org/wiki/Hemolytic-uremic_syndrome 12:14 < kanzure> what's that rare diseases website? 12:14 < kanzure> curewithme? 12:14 < kanzure> patientslikeme? 12:14 < yashgaroth> no idea 12:15 < yashgaroth> whyismyurineblue 12:15 < kanzure> damn only 1 person on patientslikeme http://www.patientslikeme.com/conditions/1500 12:16 < kanzure> http://www.patientslikeme.com/search?q=hemolytic&commit=Search 12:16 < kanzure> 10 people with autoimmune hemloytic anemia 12:18 < kanzure> ah here's a non-profit.. http://www.atypicalhus.net/ 12:19 < kanzure> haha.. "There is no cure for Atypical HUS. In fact, there is not a standard treatment, as each case is different. (Note : With the advent of Soliris, this may change over time)" 12:20 < yashgaroth> "each case is no longer different" 12:21 < kanzure> and 252 members here: http://atypicalhus.ning.com/profiles/members/ 12:21 < kanzure> so, just pick someone and give them the pitch 12:23 < kanzure> i bet you could get all of them to pitch in more than $100/mo.. so 25k/mo 12:23 < yashgaroth> depends what percentage of them are already on insurance 12:23 < kanzure> all of them. 12:23 < kanzure> but often, people can spare $100/mo 12:23 < kanzure> and you wouldn't be able to tap into their insurance money anyway 12:24 < yashgaroth> true 12:25 < delinquentme> le whut? 12:25 < kanzure> delinquentme: many rare blood diseases have treatments that cost >$300k/year 12:25 < kanzure> delinquentme: so the idea is to just do a DIY operation where they pitch in money not to buy medicine but to buy equipment or something 12:26 < delinquentme> but dont they need the meds? 12:27 < kanzure> they would use the equipment to make the meds 12:28 < uniqanomaly_> or die tryin 12:28 < yashgaroth> it's like insurance insurance, in case their insurer decides to stop paying for whatever reason 12:28 < kanzure> heh 12:28 < kanzure> yashgaroth: it would be interesting to do this by bitcoin 12:28 < delinquentme> uniqanomaly_, you're 50 cent? 12:28 < uniqanomaly_> delinquentme: sure 12:28 -!- uniqanomaly_ is now known as uniqanomaly 12:29 < uniqanomaly> 'sup 12:29 < yashgaroth> bitcoins do seem to have stabilized a little 12:29 < kanzure> i wonder if kickstarter would approve that sort of project 12:30 < kanzure> prolly not. 12:30 < yashgaroth> haha no 12:35 < kanzure> i'm not sure why someone hasn't done this already 12:35 < kanzure> let's say that a family can't get insurance, and has to pay $400k/year for this drug 12:36 < kanzure> you can /easily/ bribe some researcher to work for $10-$20k/mo to produce the antibody 12:36 < yashgaroth> they do subsidize in that case 12:36 < kanzure> hm? 12:36 < yashgaroth> alexion does 12:36 < kanzure> so then what's the point of having insurance for that, then? 12:36 < yashgaroth> making money 12:37 < yashgaroth> oh you mean patients 12:37 < kanzure> no, i mean, what is the incentive of the patient to have insurance to pay alexion 12:37 < kanzure> yes 12:38 < yashgaroth> insecurity of life, maybe 12:38 -!- marainein [~marainein@114-198-74-217.dyn.iinet.net.au] has quit [Read error: Connection reset by peer] 13:07 < Mokbortolan_1> I had an idea that might be helpful with this stuff 13:08 < Mokbortolan_1> "public x prize", where goals and prizes are implemented by the public 13:09 < Mokbortolan_1> if it were international, then non-us organizations could potentially skirt patent issues 13:10 < yashgaroth> sure, if you don't distribute the product in the US 13:12 < kanzure> Mokbortolan_1: where does the prize money come from? 13:13 < kanzure> most non-us organizations exist in countries that are signed onto WIPO, so they all believe in international patents 13:18 < Mokbortolan_1> kanzure: crowdsourced 13:19 < Mokbortolan_1> want to make a prize to develop a cheap cure for nodding disease? donate $5. There's a bit more to it in terms of prize criteria, award, and mobility of funds, but that's the basic idea 13:20 < yashgaroth> prizes don't fund research though 13:21 < yashgaroth> the space prize gave 10 mil for work that cost >100mil 13:22 < kanzure> the space prize was all funded by insurance fraud anyway 13:22 -!- delinquentme [~asdfasdf@c-24-131-65-213.hsd1.pa.comcast.net] has quit [Ping timeout: 246 seconds] 13:22 < kanzure> Mokbortolan_1: i suggest you read up on knowledge eonomy international 13:23 < kanzure> or whatever jamie love is doing these days 13:23 < kanzure> http://diyhpl.us/wiki/transcripts/open-science-summit-2010/jamie-love-knowledge-ecology-international/ 13:23 < kanzure> or the health impact fund 13:23 < kanzure> http://diyhpl.us/wiki/transcripts/open-science-summit-2010/aiden-hollis-health-impact-fund/ 13:23 < kanzure> there was also their q&a session.. http://diyhpl.us/wiki/transcripts/open-science-summit-2010/innovation-paradigm-qa/ 13:27 -!- sylph_mako [~mako@118-92-239-9.dsl.dyn.ihug.co.nz] has quit [Ping timeout: 245 seconds] 13:31 < kanzure> well this is weird.. http://keionline.org/node/1384 13:31 < kanzure> "Today India granted a compulsory license on patents held by Bayer on the cancer drug sorafenib. The Bayer price of INR 3,411,898 per year (69 thousand USD) is more than 41 times the projected average per capita income for India in 2012, shattering any measure of affordability." 13:32 < kanzure> "he 62 page decision grants the CL for the life of the patent, and grants a 6 percent royalty, which was at the high end of the UNDP 2001 royalty guidelines." 13:32 < kanzure> "Bayer tried to justify its high price by making claims of high R&D Costs, but refused to provide any details of its actual outlays on the research for sorafenib, a cancer drug that was partly subsidized by the US Orphan Drug tax credit, and jointly developed with Onyx Pharmaceuticals." 13:32 < kanzure> "Onyx told the SEC that the cost of R&D, pre-Orphan Drug tax credit, was $275 million through the 2005 FDA approval of sorafenib, including outlays on other compounds, indications that were not approved for marketing, and for expanded access trials in the United States that had limited value as scientific experiments." 13:33 < kanzure> "Because the facts in the Bayer case were extreme, the Controller was faced with a stark choice, and had the compulsory license been denied, the India statute on "reasonably affordable" pricing would have seemed like an empty protection for the public." 13:33 < kanzure> "It would have been nice for the decision to acknowledge the several compulsory licenses on drugs and medical devices that were issued in Italy and the United States in recent years." 13:34 < kanzure> http://keionline.org/node/862 http://keionline.org/node/1219 http://keionline.org/content/view/41/1 13:34 < kanzure> compulsory licensing. haha. 13:38 < Mokbortolan_1> yashgaroth: no, the prize targets would have to be crafted to be within an appropriate range 13:38 < Mokbortolan_1> I'm thinking of researchers working on their own or small teams funded by venture capitalists 13:39 < Mokbortolan_1> perhaps even just, "identify the pathogen that causes nodding disease" as a first step for that one 13:39 < Mokbortolan_1> that shouldn't take hundreds of millions to accomplish 13:39 < kanzure> i wonder if a distributed DIY production capacity for these drugs, 13:40 < kanzure> coupled with bitcoin and proper anonymization, 13:40 < kanzure> could completely cut off their revenue. 13:40 < yashgaroth> totally, but a prize implies you don't give the money out until there's a cure 13:40 < Mokbortolan_1> right 13:40 < kanzure> then you can blackmail these multi-billion dollar pharma companies for a pay off 13:40 < Mokbortolan_1> I had ideas for how to structure it, like perhaps prize goals 13:41 < yashgaroth> but if you don't stop after they pay you off, they come to kill you 13:41 < kanzure> "Look, we're manufacturing enough to treat all 1000 of your patients. You're losing $400 million a year. Our cost of operations is $100k. You give us $100 mil, and we'll stop this." 13:42 < Mokbortolan_1> no they don't "come kill you", you just die accidentally, perhaps of a heart attack, or private airplane crash 13:43 < kanzure> them subsidizing patients that don't have insurance, really puts a blocker on this 13:43 < kanzure> but they are still getting paid *somehow* 13:43 < yashgaroth> that's why you go for cancer drugs, insurance is more likely to skip those 13:44 < kanzure> really? 13:44 < kanzure> also: another soft-spot is in between "companies that have patented stuff that works" and "companies that aren't doing anything with their patents" 13:46 < yashgaroth> this is why I prefer going after drugs that have an off-label enhancement effect, which insurance won't cover and the company won't develop for 13:46 -!- sylph_mako [~mako@118-92-239-9.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap 13:48 < kanzure> oh neat: "TRIPs also provides that the requirements for a compulsory license may be waived in certain situations, in particular cases of national emergency or extreme urgency or in cases of public non-commercial use." 13:48 < kanzure> http://en.wikipedia.org/wiki/Agreement_on_Trade-Related_Aspects_of_Intellectual_Property_Rights 13:49 < kanzure> "The Doha declaration allows compulsory licenses to be issued in developed countries for the manufacture of patented drugs, provided they are exported to certain countries (principally, those on the UN's list of least-developed countries and certain other countries having per-capita incomes of less than US$745 a year)." 14:11 -!- ivan` [~ivan@unaffiliated/ivan/x-000001] has quit [Ping timeout: 276 seconds] 14:13 -!- ivan` [~ivan@unaffiliated/ivan/x-000001] has joined ##hplusroadmap 14:27 -!- jmil [~jmil@2607:f470:8:3148:7013:2583:791f:49c7] has quit [Quit: jmil] 14:39 < ParahSailin> http://www.wired.co.uk/news/archive/2012-03/27/cattle-dna-traced 14:40 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has joined ##hplusroadmap 14:42 -!- delinquentme [~asdfasdf@c-24-131-65-213.hsd1.pa.comcast.net] has joined ##hplusroadmap 14:57 -!- augur [~augur@206.196.184.189] has quit [Remote host closed the connection] 15:02 -!- deep-fried-art [~hank@71-14-122-68.dhcp.gnvl.sc.charter.com] has joined ##hplusroadmap 15:17 -!- n_bentha [~lolicon@75.111.160.104] has joined ##hplusroadmap 15:18 < n_bentha> Sorry about being a dick earlier. 15:18 < yashgaroth> did you run your ligated plasmid on a gel and/or get it sequenced over the insert? 15:19 < n_bentha> Anyway, the kanamycin plates of the plasmid w/ gene didn't have any visible colonies for after 1 days in the incubator. After sitting on the lab bench, bacterial colonies grew! 15:20 < n_bentha> But I doubt those colonies have the right plasmid in them. 15:20 < kanzure> n_bentha: it's okay i am a bigger asshole than you 15:20 < n_bentha> ^^ 15:20 < yashgaroth> wait whaddya mean sitting on the lab bench 15:20 < n_bentha> took plates out after 1 day in the incubator 15:21 < n_bentha> left it on the lab bench overnight (still covered of course). 15:21 < n_bentha> colonies the next day. 15:21 < n_bentha> (the plate was sitting on the lab bench, not me) 15:21 < yashgaroth> one would hope 15:22 < yashgaroth> so uh take me through the cloning you did 15:22 < yashgaroth> double digest, gel purify, ligation, gel purify again? 15:23 < n_bentha> Yup, pretty much. 15:23 < yashgaroth> well, any colonies you get should have the correct plasmid, no? 15:23 < n_bentha> I didn't make the plasmid...but I'm not 100% sure on that last purification step. 15:24 < n_bentha> Yes they 'should' but why did one plate w/ the original plasmid have lots of colonies after day 1 in the incubator 15:24 < n_bentha> But the one w/ the gene insertion didn't have any until day 2? 15:24 < n_bentha> Also, there were some satelite colonies on the plate w/ original plasmid on day 2. 15:24 -!- jcluck [~cluckj@cpe-67-246-45-105.nycap.res.rr.com] has joined ##hplusroadmap 15:24 < n_bentha> I guess I'll have to go make the plasmid myself. Can't rely on anyone these days. 15:25 < yashgaroth> if your gene insert has some background expression it could slow their growth, depends how toxic it is 15:26 < n_bentha> That's what I thought! 15:26 < fenn> kanzure: anything specific you want to know from DNA 2.0? 15:27 < yashgaroth> doesn't bode well for the cells when you induce the promoter though 15:27 < kanzure> how muh money they are making 15:27 < n_bentha> But I thought the gene wouldn't have an effect in bacterial cells... 15:27 < kanzure> market size 15:27 < kanzure> their production costs 15:27 -!- cluckj [~cluckj@cpe-67-246-45-105.nycap.res.rr.com] has quit [Ping timeout: 240 seconds] 15:27 < kanzure> a list of all of their customers 15:27 < kanzure> their primary products 15:27 < n_bentha> We did make a new extract of the gene from a different plant...maybe that's it 15:27 < kanzure> their roadmap/plans. 15:28 < yashgaroth> they won't divulge their customer list 15:28 < kanzure> they won't divulge the other info either :) 15:31 < n_bentha> yashgaroth...it's a chemical inducible promoter though...maybe something from creating the plasmid is activating the promoter? that might kill the cells? 15:32 < yashgaroth> possible, but if the protein is toxic then you have bigger problems 15:32 < kanzure> fenn: what's up? 15:35 < n_bentha> yeah. the transcribed protein destroys other proteins 15:40 < n_bentha> :( No I don't think that's it yashgaroth. The previous plasmids have had the same gene in them w/ a 35s promoter, so the bacteria should have been able to recognize it. 15:42 < yashgaroth> you're sure the previous ones successfully expressed the protein? 15:43 < n_bentha> Yes, we verified it w/ blots. 15:45 < yashgaroth> well I'd send it off for sequencing, get a read over the insertion site and see if the gene's there 15:45 < kanzure> how is all of this not more expensive than just making the plasmid already? :/ 15:46 < yashgaroth> sequencing costs like $10 15:46 < yashgaroth> also he has slave labor 15:48 < n_bentha> Ah, the good old days of the cotton-picking south. So many coolies to do the tedious steps for me. 15:48 < n_bentha> (i'm not racist, just making an observation of the current situation at the university i'm at) 15:49 < yashgaroth> it's actually better than slave labor, since you don't need to pay for food and housing 15:50 < n_bentha> In Capitalist America, slave pays you! 16:06 -!- AdrianG [~amphetami@unaffiliated/amphetamine] has quit [] 16:09 -!- deep-fried-art [~hank@71-14-122-68.dhcp.gnvl.sc.charter.com] has quit [Quit: deep-fried-art] 16:12 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Quit: the neuronal action potential is an electrical manipulation of reversible abrupt phase changes in the lipid bilayer] 16:13 -!- superkuh [~superkuh@c-24-118-174-49.hsd1.wi.comcast.net] has joined ##hplusroadmap 16:13 -!- superkuh [~superkuh@c-24-118-174-49.hsd1.wi.comcast.net] has quit [Changing host] 16:13 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 16:23 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 16:36 -!- jmil [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has joined ##hplusroadmap 16:49 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 16:50 < n_bentha> http://www.bbc.co.uk/news/science-environment-17539319 16:59 -!- Steel3 [81a14911@gateway/web/freenode/ip.129.161.73.17] has joined ##hplusroadmap 17:00 < Steel3> Yar har. 17:04 < delinquentme> interviews interviews 17:08 -!- Steel3 [81a14911@gateway/web/freenode/ip.129.161.73.17] has quit [Ping timeout: 245 seconds] 17:13 < fenn> off to sunny sunnyvale 17:14 < kanzure> seeya 17:18 -!- Steel3 [81a14911@gateway/web/freenode/ip.129.161.73.17] has joined ##hplusroadmap 17:24 -!- deep-fried-art [~hank@71-14-122-68.dhcp.gnvl.sc.charter.com] has joined ##hplusroadmap 17:27 < Vicarious> hi 17:31 < Steel3> how goes, vicarious? 17:37 -!- AdrianG [~amphetami@unaffiliated/amphetamine] has joined ##hplusroadmap 17:37 -!- deep-fried-art [~hank@71-14-122-68.dhcp.gnvl.sc.charter.com] has quit [Remote host closed the connection] 17:37 -!- deep-fried-art [~hank@71-14-122-68.dhcp.gnvl.sc.charter.com] has joined ##hplusroadmap 17:43 -!- Mariu [Jimmy98@89.41.57.33] has quit [Quit: brb] 17:52 -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap 18:03 -!- strages_home [~strages@adsl-98-81-13-145.hsv.bellsouth.net] has joined ##hplusroadmap 18:24 < delinquentme> Société Mathématique de France kanzure 18:24 < delinquentme> that screams "SANITIZE ME" 18:24 < Mariu> xD 18:40 -!- wudles [~wudles@gateway.secureinstrument.com] has joined ##hplusroadmap 18:40 < kanzure> hi wudles 18:41 < wudles> Hi, just logging in to work... ;) 18:41 < kanzure> wudles: what is planetx.com? 18:42 < thesnark> NIBIRU 18:42 < kanzure> hi thesnark 18:42 < wudles> scifi / transhuman wiki ... homo excelsior. 18:42 < thesnark> hey there 18:43 < kanzure> wudles: okay. we do work on a transhumanist technology roadmap, including cheap lab equipment and biohacking and other practical things 18:43 < kanzure> deep-fried-art: sup 18:44 < n_bentha> sweet, Knights of Sidonia got an update. thanks to whoever posted the link for that 18:45 < Steel3> It's a great story 18:45 < Steel3> I love nihei's work 18:45 < n_bentha> but omg it switched to left to right now 18:47 < n_bentha> wait nevermind... 18:48 < strangewarp> I keep misreading "futurists" as "fursuits 18:48 < strangewarp> damn you, internet 18:48 < Steel3> welp 18:48 < Steel3> lol 18:48 < n_bentha> LOL 18:49 < Steel3> strangewarp, where are you located out of? 18:49 < strangewarp> Steel3: Boulder, CO. Currently in a bit of a rut in my life. Kind of a bullshit town if you're not monied. 18:49 < Steel3> ah 18:50 < Steel3> how far is boulder from fort collins? 18:50 < strangewarp> Hmmm 18:51 < strangewarp> Over to I-25, and then north for a while... I'm not really sure, haven't had any reason to drive there 18:51 < strangewarp> I'd say 30 minuets to an hour 18:51 < Steel3> ah 18:51 < Steel3> I have a friend down there 18:52 < strangewarp> oh nice. Fort Collins is where CSU is located, so it has a decent party scene (I am told) and it's launched a couple decent bands, more than you'd expect out of the city's population 18:53 < Steel3> yeah, my friend is working on particle accelerator design 18:53 < Steel3> at csu 18:53 < strangewarp> oh rad 19:01 -!- _sol_ [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 248 seconds] 19:02 -!- SolG [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap 19:05 < deep-fried-art> kanzure: hey 19:06 < kanzure> get my email? 19:07 < deep-fried-art> yep, just replied 19:09 < kanzure> ah okay 19:09 < kanzure> yeah you had a whole task force mobilizing against you. 19:09 < Steel3> wtf happened 19:10 -!- n_bentha [~lolicon@75.111.160.104] has left ##hplusroadmap [] 19:11 < deep-fried-art> yea... I do understand 19:11 < deep-fried-art> also, the notion of a task force sounds pretty scary 19:11 < kanzure> http://web.archive.org/web/20080708235522/http://www.fbi.gov/hq/nsb/wmd/images/hrtppe.jpg 19:13 < deep-fried-art> but not as scary as that picture lol 19:17 -!- delinquentme [~asdfasdf@c-24-131-65-213.hsd1.pa.comcast.net] has quit [Quit: Leaving] 19:18 -!- Steel3 [81a14911@gateway/web/freenode/ip.129.161.73.17] has quit [Ping timeout: 245 seconds] 19:21 < fenn> dna2.0 website says "tens of thousands of genes synthesized" assuming 1.5kb average that's $0.80/bp*1.5kbp*20000 = $24million in revenue 19:21 < kanzure> per day? 19:21 < fenn> total 19:22 < kanzure> how long have they been around? three years now? 19:22 < fenn> i guess they're not a public company 19:22 < fenn> right 19:22 < fenn> since 2003 19:23 < kanzure> so about 2500 genes per year 19:24 < kanzure> that's depressing. 19:29 < fenn> " Rest assured that all your genes are made in sunny California, 100% accurate and intellectual property compliant." 19:29 < kanzure> intellectual property compliant! oh goodie. 19:29 < fenn> there's actually a lot of information on their website 19:31 < fenn> heavily paraphrased transcript http://fennetic.net/irc/2011-03-28_dna2.0_biocurious 19:33 < kanzure> hoooray 19:34 < kanzure> wtf 15 day turn around? 19:34 < kanzure> 2 kb in 10 days? 19:35 < kanzure> meh "we use non-template PCR (overlapping oligos) with some trade secret optimizations" 19:35 < fenn> their rush is 5 days, apparently everything takes a couple days and they want to have a buffer for errors and re-doing the process 19:36 < kanzure> how do they explain IDT having <5 days 19:36 < fenn> smaller gene products i guess 19:36 < fenn> "we differentiate on the science" presumably idt doesn't have as good optimization algos 19:37 < kanzure> deep-fried-art: just don't do anything illegal and you'll be fine 19:37 < kanzure> it's standard really.. 19:38 -!- n_bentha [~lolicon@75.111.160.104] has joined ##hplusroadmap 19:41 < deep-fried-art> I understand... I forgot for a moment that, on one level, it's serious business 19:41 -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Ping timeout: 276 seconds] 19:44 < deep-fried-art> fenn: thanks for that transcript. I was hoping I wouldn't have to sift through that whole website 19:50 -!- nmz787 [43f2b117@gateway/web/freenode/ip.67.242.177.23] has joined ##hplusroadmap 19:50 < nmz787> yo 19:50 < kanzure> hey nmz787 19:50 < kanzure> fenn: ping? 19:50 < kanzure> we're trying to figure out wtf w/ laser cutters versus soft lithography 19:50 < fenn> pong 19:51 < nmz787> ding 19:51 < fenn> soft lithography = stamping a mask? 19:51 < kanzure> wowowow halcyon is liquidating 19:51 < kanzure> BUY EVERYTHING 19:51 < nmz787> more like making a mold, then laying elastomer on top 19:51 < nmz787> then peel off when rubbery 19:51 < fenn> eh? 19:53 < nmz787> ? 19:53 < yashgaroth> oh shit you weren't kidding, did musk stop giving them money? 19:53 < kanzure> musk didn't give them the latest round of funding 19:54 < nmz787> i'm not finding anything on it on google news 19:54 < kanzure> nmz787: i think you need to explain the context 19:54 < kanzure> for fenn 19:54 < nmz787> oh 19:54 < kanzure> yeah, the news takes a few hours to react 19:54 < kanzure> if we hurry, we can get the equipment 19:54 < yashgaroth> did they have any centrifuges 19:54 < kanzure> they had all kinds of things. yes 19:54 < nmz787> link to anything? 19:55 < kanzure> full machine shop. full biology lab. full chem lab. 19:55 < kanzure> anselm's partner was raiding halcyon today, getting some liquid handlers 19:55 < nmz787> fenn: basically we need a way to make microstructures at least cost 19:55 < n_bentha> 0_o 19:56 < nmz787> fenn: one method that looks good for prototyping microfluidics is cut-through then transfer methd 19:56 < nmz787> PDMS is layed onto acrylic, then cut through with a laser cutter 19:56 < nmz787> with enough power that the beam also chews up some acrylic 19:56 < fenn> "eh?" was about halcyon 19:56 < nmz787> ensuring the PDMS isn't curved at the bottom 19:57 < nmz787> then the PDMS is bonded with an uncut sheet and peeled off the acrylic 19:57 < nmz787> on the cheap end of laser cutters we have chinese models on ebay for $800 19:57 < nmz787> they dont have a small spot size 19:58 < nmz787> which is accomplished by first expanding the beam size, then focusing it again 19:58 < fenn> for such a small spot i'd imagine a low power diode would do fine 19:58 < nmz787> (wider beam can be focused more tightly) 19:58 < nmz787> (more easily) 19:58 < fenn> any reason we need a high power (>25W) laser? 19:58 < nmz787> nah 19:59 < nmz787> well diode beams aren't as nice as CO2 19:59 < nmz787> or rather non-diode lasers 19:59 < fenn> because they're not circular? 19:59 < nmz787> because the diodes tend to be less coherent, and yeah not circular 19:59 < fenn> elliptical gaussian because of side-exit 19:59 < fenn> that can be corrected btw 19:59 < nmz787> or adding multiple diodes into one light pipe 20:00 < nmz787> get weird shapes 20:00 < fenn> i like diodes because they're small and lightweight thus you don't need fancy optics 20:00 < nmz787> but anyways CO2 is better for acrylic 20:00 < nmz787> as you said to correct the beam, you need more optics than with a non-diode laser 20:00 < fenn> so, what's the point of trying to do it all in one step, instead of etching in a solution? 20:01 < nmz787> how do you control where you etch? 20:01 < fenn> you mask off parts of the glass 20:01 < nmz787> what glass? 20:01 < fenn> photo polymerizable resin 20:01 < fenn> huh? 20:01 < nmz787> right that's the other option 20:01 < nmz787> soft-lithography 20:01 < kanzure> "hacker dojo is fucked. they got a fire code violation. gotta spend 250k to upgrade fire systems" 20:01 < fenn> i mean glass instead of acrylic 20:01 < nmz787> which we could either send out for 20:02 < nmz787> or use LCD 20:02 < fenn> kanzure: it's overblown, mtn view is just trying to extract money from them 20:02 < nmz787> toner on transparency sucks 20:02 < fenn> no, use the laser to polymerize a mask onto glass directly 20:02 < fenn> like is done for PCB etching 20:02 < nmz787> most resins are in the UV 20:02 * fenn shrugs 20:02 < nmz787> excimer lasers are really expensive 20:03 < n_bentha> 250k?!?! 20:03 < nmz787> maybe there are better/other resins 20:03 < fenn> i believe there are UV diode lasers 20:03 < fenn> anyway there are blue light curing resins and other more fancy stuff 20:03 < fenn> the reason PCB's are traditionally green is they use an IR cure epoxy for solder mask 20:04 -!- kvltist [~Kvltist@p5B33F40D.dip.t-dialin.net] has quit [Quit: Computer has gone to sleep.] 20:04 < fenn> so, basically i've heard that pdms sucks because it's too poroous 20:04 < fenn> and i'm trying to figure out how to do this without pdms 20:04 < kanzure> i don't think you can do pressure valves with glass tho 20:05 < nmz787> nope 20:05 < nmz787> PDMS is great for some things 20:05 < nmz787> other things porosity becomes an issue 20:05 < nmz787> or tricky 20:06 < fenn> ok so what's the minimum resolution needed to start with? 20:06 < nmz787> i was thinking 25 micron channels 20:06 < kanzure> it would be nice to have 1 micron control but w/e 20:06 < nmz787> http://www.kellerstudio.de/repairfaq/sam/laserioi.htm#ioicdf 20:06 < nmz787> yeah 20:07 < nmz787> well channel width isn't directly the same as beam control 20:07 < kanzure> yeah it depends on whether we're doing cut-through channels or just etching with the beam into the material to make some depth 20:07 < nmz787> 1000 dpi gets you 25.4 microns / step of a motor 20:08 < fenn> cant we just use a microscope and shoot the laser through the eyepiece? 20:08 < nmz787> possibly 20:08 < nmz787> i guess depends on the glass 20:08 < nmz787> if its blue, prob 20:08 < fenn> yeah glass is UV absorbent 20:08 < nmz787> blue light 20:09 < fenn> i've forgotten everything i knew about lasers 20:09 < nmz787> 3 axis CNC setup basically is needed 20:09 < fenn> an off the shelf CNC won't get you 1 micron 20:09 -!- Mariu [Jimmy98@89.41.57.33] has quit [] 20:09 < kanzure> i was just bullshitting with 1 micron, we can probably get away with something bigger 20:09 < fenn> 50 micron is typical, 10 if it's super fancy 20:09 < kanzure> but it would be nice to have smooth curves at some resolution 20:10 < fenn> yes 20:10 < nmz787> using 1/4 40 screws, one turn moves 15.87 microns 20:10 < nmz787> motors can be microstepped to 256 places 20:10 < fenn> pixelated channels seems wonky 20:10 < nmz787> atoms are picels 20:10 < nmz787> pixels 20:10 < fenn> yes differential threading can do arbitrary step/movement ratio 20:11 < fenn> basically one thread goes forward the other goes backwards, the difference is the travel 20:11 < nmz787> 1/4 40 screws are easy to come by 20:11 < nmz787> and we can add a simple interferometer using a photodiode to feedback to the controller if better precision is needed 20:12 < fenn> actually i might be wrong about the repeatability of typical CNC's 20:12 < kanzure> i doubt 50 micron is typical for cnc? 20:12 < fenn> usually they're specified in terms of accuracy over the entire bed 20:13 < nmz787> this is basically what i just described http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&ved=0CE8QFjAB&url=http%3A%2F%2Fwww.himt.de%2Fen%2Fproducts%2Fdwl66fs.php&ei=I9FzT-uXEoTk0QHvw93_Ag&usg=AFQjCNEsU4of4ZQEy9cz5UxegCAtK_V9dw&sig2=TSeSKWc_XXVq_aE1fSd64w 20:13 < nmz787> err 20:13 < fenn> i presume we don't really care about accuracy as long as it's repeatable error 20:13 < nmz787> http://www.himt.de/en/products/dwl66fs.php 20:13 < nmz787> they have a 6 month lead time or something 20:13 < nmz787> and i think they want around $250k 20:13 < fenn> that looks like overkill 20:14 < nmz787> for something that could almost be done using old CDROM drives 20:14 < fenn> um 20:15 < fenn> the reason cd-rom works is it uses closed-loop analog(?) feedback from the track reflection 20:15 < fenn> using dvd diode is an interesting idea though 20:15 < n_bentha> 250k! You could upgrade your fire-system for that! 20:16 < fenn> er blu-ray, whatever is higher energy 20:16 -!- thesnark [~thesnark@unaffiliated/thesnark] has quit [Remote host closed the connection] 20:17 -!- wudles [~wudles@gateway.secureinstrument.com] has quit [Quit: Nettalk6 - www.ntalk.de] 20:17 < fenn> cd-rom is 787nm, dvd is 657nm, blu-ray is 405nm 20:17 < nmz787> no i meant cdrom for the gantry and rubber bushings 20:17 < nmz787> http://www.yamahamultimedia.com/yec/tech/discta2_01.asp 20:18 < nmz787> right cd/dvds use analog feedback via the spiral, but thats why i added in the interferometer 20:18 < fenn> i'm wary of interferometry 20:18 < nmz787> lol 20:18 < nmz787> why? 20:18 -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap 20:19 < nmz787> http://www.metacafe.com/watch/1381543/laser_interferometer_homemade_for_20/ 20:19 < fenn> they're generally a pain in the ass 20:19 < nmz787> put a photodiode at the image in this setup 20:19 < nmz787> and you get a sine wave out 20:19 < fenn> bleh i can't see that video 20:19 < nmz787> add a comparator and you have digital ticks out 20:21 < nmz787> the worst part is vibration from the room or the system (motors) 20:21 < nmz787> or rather what you have to watch out for 20:22 * fenn eyes the 8:40 caltrain 20:23 < nmz787> unless i find something better 20:23 < nmz787> looks like i'll be diving into this 20:23 < nmz787> http://www.maxreason.com/software/optics/opus.html#overview 20:24 -!- AdrianG [~amphetami@unaffiliated/amphetamine] has quit [Read error: Connection reset by peer] 20:25 -!- jmil [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has quit [Quit: jmil] 20:25 -!- AdrianG [~amphetami@unaffiliated/amphetamine] has joined ##hplusroadmap 20:25 < fenn> ok gotta go or i'll be puttering here for another hour 20:28 -!- nmz787 [43f2b117@gateway/web/freenode/ip.67.242.177.23] has quit [Quit: Page closed] 20:29 < kanzure> aw don't disconnect 20:47 -!- superkuh_ [~superkuh@2607:f358:1:fed5:22:0:57a0:e6de] has joined ##hplusroadmap 20:47 -!- superkuh_ [~superkuh@2607:f358:1:fed5:22:0:57a0:e6de] has quit [Changing host] 20:47 -!- superkuh_ [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 20:48 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Quit: the neuronal action potential is an electrical manipulation of reversible abrupt phase changes in the lipid bilayer] 20:57 -!- deep-fried-art [~hank@71-14-122-68.dhcp.gnvl.sc.charter.com] has quit [Quit: deep-fried-art] 21:01 -!- superkuh_ is now known as superkuh 21:03 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has joined ##hplusroadmap 21:22 -!- Mokbortolan_ [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has joined ##hplusroadmap 21:42 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Remote host closed the connection] 21:43 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has joined ##hplusroadmap 21:52 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Quit: Leaving.] 21:55 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has joined ##hplusroadmap 21:56 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Ping timeout: 246 seconds] 21:58 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 21:59 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Client Quit] 22:14 -!- n_bentha [~lolicon@75.111.160.104] has left ##hplusroadmap [] 22:39 -!- jenzebubble [jen@173-19-241-225.client.mchsi.com] has quit [Ping timeout: 260 seconds] 22:58 -!- AdrianG [~amphetami@unaffiliated/amphetamine] has quit [] 23:20 -!- jennicide [jen@173-19-241-225.client.mchsi.com] has joined ##hplusroadmap 23:22 -!- delinquentme [~asdfasdf@c-24-131-65-213.hsd1.pa.comcast.net] has joined ##hplusroadmap 23:27 * kanzure sleeps 23:34 -!- Vicarious [diepfriet@v.icario.us] has quit [Ping timeout: 260 seconds] 23:42 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has quit [Quit: jmil] 23:52 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Ping timeout: 244 seconds] 23:56 -!- ParahSailin [~parah@unaffiliated/parahsailin] has quit [Ping timeout: 240 seconds] --- Log closed Thu Mar 29 00:00:12 2012