--- Log opened Sat Apr 14 00:00:34 2012
00:03 < diginet> urgh, have a protein synthesis machine would be bloody awesome
00:05 < diginet> *having
00:17 < kanzure> go get one..
00:17 < kanzure> http://www.ebay.com/itm/Applied-Biosystems-Peptide-Synthesizer-432A-/230695576437?pt=LH_DefaultDomain_0&hash=item35b6872375#ht_4491wt_1274
00:17 < kanzure> $1k
00:18 < kanzure> haha this is so ridiculous
00:18 < kanzure> http://www.ebay.com/itm/Applied-Biosystems-Expedite-8909-Nucleic-Acid-Synthesizer-/280777705321?pt=LH_DefaultDomain_0&hash=item415fa7c769#ht_500wt_1289
00:18 < diginet> I doubt that is capable of synthesizing any peptide long enough for my purposes
00:18 < kanzure> that's almost as tall as i am
00:19 < kanzure> "The Expedite™ 8909 Nucleic Acid Synthesis System is an economical, versatile platform that synthesizes DNA and RNA oligonucleotides as well as peptide nucleic acids"
00:19 < kanzure> economical? bah
00:20 < kanzure> oh man $639 milligen synth on ebay..
00:20 < kanzure> and for $750+shipping you can get one with a manual? damn
00:21 < diginet> that's a. . .really good deal
00:21 < diginet> like, if I had the money I would totally buy that
00:21 < kanzure> yeah i think i'll get it
00:21 < diginet> seriously?
00:21 < kanzure> sure why not
00:21 < diginet> could you synthesize for me?
00:22 < kanzure> no it probably doesn't work
00:22 < diginet> ohhhhh
00:22 < kanzure> http://www.ebay.com/itm/Millipore-MilliGen-Cyclone-Plus-DNA-Synthesizer-8400-/130530382334?pt=LH_DefaultDomain_0&hash=item1e643791fe#ht_653wt_1274
00:22 < kanzure> "Millipore MilliGen/Biosearch DNA Synthesizer. Model 8400 Cyclone Plus. Unit comes with Operators Manual, Chemistry Cartridge: H-Phosphate (Version: PV1.07) and Power Cord. Unit powers on to a bright and clear display and responds to commands. We do not have the resources to test further but are giving a 14-day warranty."
00:22 < diginet> well, do you think you could fix it?
00:22 < kanzure> if i buy it i will send it to fenn or nmz787
00:22 < diginet> ahhhh
00:22 < diginet> how nice of you!
00:23 < diginet> I wonder if there is a way to catalyse peptide bonding besides the way ribosomes do it
00:23 < kanzure> it's in arizona.. shipping's a pain
00:25 < kanzure> well they end in >10 days each so i'll wait on it and see what the others think
00:26 < diginet> ah okay
00:26 < diginet> but anyway
00:28 < diginet> do you think there's any reasonable way to catalyze peptide bonds without ribosomes?
00:29 < yashgaroth> you mean chemical synthesis? we covered that
00:29 < diginet> well yes, but you said that's absurdly slow
00:29 < yashgaroth> it's still the state of the art if you don't like ribosomes
00:30 < diginet> I understand, but I'm saying how could it be improved to at least approach the speed of a ribosome
00:31 < kanzure> yashgaroth: yea or nay on that synthesizer
00:32 < yashgaroth> uhhhhh well I do like biosearch
00:38 < yashgaroth> why don't you guys build a microfluidic oligo synthesizer :V
00:38 < kanzure> working on it
00:39 < kanzure> i'm allowed to have toys too you know..
00:39 < yashgaroth> heh
00:39 < kanzure> if my toys are pokemon and dna equipment then so be it
00:40 < kanzure> hrm.. $4900 SEM scope
00:47 < kanzure> oh neat. lots of el cheapo syringe pumps.
00:52 < diginet> so why is ribosomal cell free expression slower than cellurar?
00:54 < kanzure> cytoplasm/environment differences
00:54 < kanzure> that's my understanding at least.
00:55 < yashgaroth> who said it was slower
00:55 < diginet> it isn't slower?
00:55 < diginet> I thought it was?
00:56 < yashgaroth> nah
00:56 < diginet> ohhh
00:56 < diginet> hmmm
00:56 < diginet> could cell free be competitive for macroscopic production?
00:58 < yashgaroth> could? maybe. is? no
00:58 < diginet> why is it not as of now?
00:58 < yashgaroth> cellular production is more efficient
00:58 < kanzure> life forms optimized to make things for you.
00:59 < yashgaroth> if something's a shit expressor in cells, it's usually due to problems related to folding anyway, which in vitro doesn't solve
01:00 < yashgaroth> its main use now is dna/rna display panning, or at least that's all I'd use it for
01:00 < diginet> ah, I see
01:01 < diginet> if someone figured out a way to make a machine which could reliably synthesize proteins of arbitrary length (to an extent) that would be. . .wow
01:01 < diginet> just amazing
01:01 < yashgaroth> Jeff McEvolution did that a while ago
01:02 < yashgaroth> try doing silk proteins in vivo first, then start worrying about in vitro if it doesn't work
01:02 < diginet> I know, I'm just saying it would be cool
01:02 < kanzure> try not killing your gfp culture first
01:03 < kanzure> night
01:13 < diginet> well, the good news is that I found out that purifying the proteins is actually really easy
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01:13 < diginet> I think duckweed should prove a very good expression system
01:13 < diginet> I need to do some experiments on optimizing growth though
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01:21 < diginet> http://atp.ncifcrf.gov/proteins-and-proteomics/protein-expression-laboratory/pel-services/high-yield-protein-synthesis-using-cell-free-protein-expression-system
01:21 < diginet> hmmm
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03:25 < Mariu> I have to split, laters
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03:45 < diginet> wow, synthetic life is really interesting
03:45 < diginet> I would be more interested in synthetic life that differs from natural life, like that uses a different nucleic acid or something
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05:39 < diginet> you know what we need? open source retrosynthetic analysis
05:42 < delinquentme> and  you use legal to protect open source?  I just had a thought that say you being supplying to a manufacturer and they sign an exlcusive agreement for say a year
05:42 < delinquentme> on whatever youre building ...
05:43 < delinquentme> that can act to support dev of your open source hardware
05:43 < delinquentme> http://en.wikipedia.org/wiki/Retrosynthetic_analysis
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06:24 < kanzure> beep boop
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09:01 < delinquentme> meh! gmail is being
09:01 < delinquentme> lame
09:14 < diginet> is 1 g/L a day a reasonable yield to expect of a recombitant protein expression system?
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09:50 < kanzure> looks like qcad has gone commercial
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09:50 < kanzure> and the open source project forked to http://librecad.org/
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10:00 < kanzure> juri_'s building an open source electron microscope
10:01 < kanzure> he's asking for $250k but i haven't seen his BOM
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12:38 < kanzure> hi _F7_
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13:03 < delinquentme> surely there are standards established for what volume constitutes a droplet at 1 atmosphere
13:08 < kanzure> a few picoliters
13:08 < kanzure> is a microdroplet.
13:09 < kanzure> i'm pretty sure there's a maximum size due to surface tension vs. gravity considerations
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14:21 < _F7_> test
14:21 < _F7_> Okay cool
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15:13 < augur> anyone read society of mind?
15:22 < kanzure> one of marvin's students was in here the other day, does that count :p
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16:41 < ParahSailin> i dont think http://blog.opensourceecology.org/2010/12/open-source-aluminum/ is workable
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17:35 < thylne> [20:34] <noumena> Humans haven't lived in industrial societies for that much of our history
17:35 < thylne> [20:34] <EkurioX> Amazing isn't it... You should listen to Al gore... Smart dude.
17:35 < thylne> [20:35] <mrdata> we reached the singularity in 2004
17:35 < thylne> >_>
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17:42 < strangewarp> thylne: god, that is painful
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18:45 < delinquentme> kanzure, how do I get solidworks legitimate and cheap?
18:45 < delinquentme> do they sell old versions for less?
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19:09 < kanzure> delinquentme: become a student somewhere
19:09 < kanzure> or join a company that pays for a copy
19:10 < kanzure> :/
19:10 < delinquentme> commerical license
19:10 < delinquentme> commercial **
19:10 < kanzure> well you could always pay them directly
19:10 < kanzure> have you considered using alibrecad instead?
19:12 < delinquentme> i think i've got an old license
19:14 < kanzure> also if you draw on a napkin and squint reallly hard it sorta looks like actual work :/
19:15 < delinquentme> CHECK
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19:45 < thylne> strangewarp  the stupid was making my eyes burn  :D
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22:01 < kanzure> Adifex: hi
22:01 < Adifex> wazzup
22:02 < Adifex> this place seemed pretty dead last time I logged on, is there anyone here?
22:02 < kanzure> yes we're all just afraid of you
22:02 < kanzure> how do you know us?
22:09 < Adifex> oh I don't. I'm just a lurker here mostly.
22:21 < ybit> Adifex: utdallas, aye?
22:21 < ybit> what are you studying there?
22:26 < Mariu> Adifex, how do you know of us ?
22:26 < katsmeow-afk> http://laughingsquid.com/choc-creator-the-chocolate-3d-printer-is-now-available-to-purchase/
22:27 < kanzure> katsmeow-afk: i prefer my chocolate to be 6-axis milled
22:28 < Mariu> :D
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--- Log closed Sun Apr 15 00:00:36 2012