--- Log opened Tue Aug 21 00:00:00 2012 00:05 < nmz787> http://wholebraincatalog.org/ 00:05 -!- HariSeldon [~Shehrazad@88.243.141.196] has joined ##hplusroadmap 00:06 -!- Guest19125 [~quassel@64.31.59.70] has quit [Read error: Operation timed out] 00:06 -!- Charlie [~quassel@64.31.59.70] has joined ##hplusroadmap 00:07 -!- Charlie is now known as Guest56798 00:08 -!- Haruspex [~Shehrazad@88.243.141.196] has quit [Ping timeout: 240 seconds] 00:08 < nmz787> this video is quite good http://www.cwgp.org/drew_berry.php 00:10 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Quit: rmcl] 00:16 -!- sylph_mako [~mako@213.195.252.27.dyn.cust.vf.net.nz] has joined ##hplusroadmap 00:31 -!- SolG [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap 00:32 -!- _sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 245 seconds] 00:33 -!- cutbert [~nima@2001:4830:1100:104::2] has quit [Ping timeout: 246 seconds] 00:33 -!- cutbert [~nima@cl-261.bos-01.us.sixxs.net] has joined ##hplusroadmap 00:44 -!- cutbert [~nima@cl-261.bos-01.us.sixxs.net] has quit [Ping timeout: 250 seconds] 00:44 -!- cutbert [~nima@cl-261.bos-01.us.sixxs.net] has joined ##hplusroadmap 01:14 -!- ThomasEgi [~thomas@p5B13B6DE.dip.t-dialin.net] has joined ##hplusroadmap 01:14 -!- ThomasEgi [~thomas@p5B13B6DE.dip.t-dialin.net] has quit [Changing host] 01:14 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 01:15 -!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has quit [] 01:23 -!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has joined ##hplusroadmap 01:26 -!- fenn_ is now known as fenn 01:27 < fenn> i'm a big fan of drew berry's work. downloaded all his stuff, it's around here somewhere.. 01:31 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Read error: Connection refused] 01:33 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap 01:36 < fenn> "a day made of glass" is more about touchscreens and gestural interfaces than anything 01:36 < fenn> and, it makes me want to puke. EOM 01:37 < fenn> "3d projection"? wtf is that? 01:38 < fenn> why are there humans driving the cars? 01:39 < fenn> why do the kids have backpacks when they have these super fancy smartphones? 01:40 -!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Quit: Leaving.] 01:44 < strangewarp> The humans are driving cars because of shitty moral-panic-driven laws against driverless cars 01:44 < strangewarp> The kids have backpacks for bottles of alcohol 01:44 < strangewarp> gosh it's obvious 01:46 < fenn> this video would make a lot more sense if it had been made by samsung 01:46 < fenn> they're already making those see through window displays 01:47 < fenn> "driverless cars" reminds me of "horseless carriage" 01:47 < fenn> if the tablets werent made of glass they wouldnt need cases 01:48 < fenn> and a glass dashboard seems like a terrible idea 01:53 -!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 01:55 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 246 seconds] 01:59 -!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap 02:00 < nmz787> fenn, so I heard microstepping is bad if you use a motor that's designed for high holding torque 02:00 < nmz787> because the magnetic field doesn't fall off from the tooth with the cosine/sine rule 02:02 < fenn> yes, i told you about that a long time ago 02:02 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 244 seconds] 02:03 < fenn> i know the guy who designed the lookup table to compensate for that 02:03 < fenn> same lookup table is used in almost every stepper controller chip, but it's not perfect 02:04 < nmz787> this guy is where i first saw it http://users.ece.utexas.edu/~valvano/Datasheets/StepperMicrostep.pdf 02:04 < nmz787> he has a lot of other papers too if you just go up the URL 02:04 < nmz787> http://users.ece.utexas.edu/~valvano/Datasheets/StepperSelection.pdf 02:04 < nmz787> well this was saying that you just shouldn't use high-holding torque motors 02:05 < nmz787> if you want good microstepping 02:05 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Ping timeout: 276 seconds] 02:05 < fenn> so you want something like slo-syn instead? 02:06 < fenn> actually i don't know which kind of motors are most linear 02:06 < nmz787> what's that? 02:06 < fenn> an old motor design you find in a lot of scrapped scientific equipment, it's usually painted grayish teal, and looks like a fat cylinder 02:07 < nmz787> hmm http://www.contraptor.org/forum/t-273788/micro-cnc-laser 02:07 < fenn> they can be driven by sinusoidal AC or using square waves 02:07 < fenn> heh contraptor, i forgot about that guy 02:08 < nmz787> so why didn't you choose to build the laser_etcher to look like this? http://cncestablishment.com/wp-content/uploads/2011/11/cnc-engraving-machine.jpg 02:09 < fenn> i explained all that in the last email 02:09 < fenn> i could be wrong. only one way to find out 02:10 < nmz787> hmm 02:10 < fenn> the contraptor forum etcher looks pretty good 02:10 < nmz787> i don't really see an explanation of this particular config 02:10 < nmz787> you talked about MDF 02:10 < nmz787> and oil bearings 02:10 < fenn> okay what is the exact question then? 02:11 < nmz787> well that image that i just posted *looks* different than your SCAD rendering 02:11 -!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has quit [] 02:11 < fenn> in many ways 02:12 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 02:12 < fenn> it's not a rendering, for one 02:12 < nmz787> uhh 02:12 < nmz787> a screenshot of a rendering? 02:13 < fenn> you mean why do i have two sets of ways bolted to the same plate? 02:13 < fenn> or "why didnt you make a gantry router" 02:13 < nmz787> well they both apply 02:13 < fenn> (also those things suck as routers) 02:13 -!- augur_ [~augur@208.58.5.87] has joined ##hplusroadmap 02:13 < nmz787> yeah so both of those questions 02:14 < fenn> 1) for rigidity, 2) i thought it would be better to keep the laser tube stationary if we decided to use a CO2 laser 02:15 -!- augur [~augur@208.58.5.87] has quit [Read error: Connection reset by peer] 02:15 < fenn> adding flying optics would complicate the design 02:15 < nmz787> sure 02:16 < nmz787> so is that model complete? 02:16 < fenn> no 02:16 < nmz787> if I got openSCAD running could I follow it as a pictorial guide to getting it together? 02:16 < fenn> yes 02:16 < fenn> you'd have to calculate some dimensions though 02:16 < fenn> as far as i can tell there are no "measure this distance" tools in openscad 02:16 < nmz787> so what's missing, if its not completer 02:16 < nmz787> complete* 02:17 < nmz787> or is it those exact distances that is missing? 02:17 < fenn> it's missing the top and bottom plates, the bolt hole locations, the leadscrews and leadnuts and bearing mounts and stepper motor mounts, and it needs some kind of right angle bracket to hold the laser optics 02:17 < nmz787> did you just eyeball the structure with no real measurments? 02:18 < fenn> the structure is eyeballed, but the parts are modeled after real parts (or the provided cad drawings at least) 02:18 < fenn> it should look like the rendering if built to the dimensions in the openscad file 02:18 < nmz787> for the optics you mean anything that could hover about the setup? 02:18 < fenn> right 02:19 < fenn> i was thinking some kind of optical breadboard at 90 degrees to the motion stage 02:19 < nmz787> hmm 02:19 < nmz787> the screws, bearings and motor mounts are the only things I think I am not clear on then 02:20 < nmz787> rather, how those items connect together 02:23 < fenn> hm, i hadn't tested to make sure all the dimensions work the way i had imagined 02:23 < fenn> but it should be close enough to take out any vertical slop with shims 02:24 < nmz787> hmm, any chance you'd be interested in adding the screws and bearings and motor mounts to the CAD ? 02:25 < fenn> do you have the file support_block.scad in your working dir? 02:25 < fenn> just wondering if i had pushed it earlier 02:28 < nmz787> its here http://diyhpl.us/laser_etcher/ 02:28 < fenn> ok 02:31 -!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has joined ##hplusroadmap 02:31 < fenn> you should add links to any whitepapers you find relevant in the laser_etcher.mdwn 02:32 < nmz787> ahh, ok 02:32 < fenn> i was considering using a "flexure bearing" to connect the lead nut and the moving part of each axis 02:33 < fenn> but it turns out one can't simply buy his way into mordor 02:33 < fenn> i mean, buy a flexure bearing 02:35 < nmz787> hmm 02:35 < fenn> the point would be to prevent rocking deflection perpendicular to the axis of motion from a slightly eccentric leadscrew 02:36 < fenn> this was a problem on the makerbot Z axis at one point and solved in a similar way 02:36 < nmz787> so is it just a bearing in a rubber block? 02:36 < fenn> no 02:37 < fenn> it only flexes in one direction, or two directions 02:37 < fenn> one moment, the internet is not cooperating 02:38 -!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has quit [] 02:39 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 02:43 < fenn> blarg. well this is the best pic of it i can find: http://www.thingiverse.com/thing:2151 http://www.thingiverse.com/image:36713 02:43 < fenn> it's not a particularly brilliant design but i hope you get the idea 02:43 < fenn> the vertical rigidity is greater than the horizontal rigidity 02:44 < fenn> so the nut can move around in X and Y without affecting the Z position 02:46 < fenn> oh, the makerbot wobble arrester is only really designed to flex in one direction 02:48 < nmz787> hmm, so you can't buy one of those? 02:49 < fenn> not that i can find 02:49 < fenn> it's a trivial waterjet job though 02:49 < nmz787> can we just use that design, printed locally or from shapeways? 02:50 < nmz787> or is that design inadequate? 02:50 < fenn> it would need to be modified to fit our nut, and then might as well just redesign it from scratch 02:51 < fenn> it may not be necessary at all though 02:51 < nmz787> seems like its just a spring though, which on the makerbot Z aren't there multiple screws? 02:51 < fenn> i just wanted to convey the concept to you 02:51 < nmz787> where we'll only have 1 screw in any direction 02:52 < fenn> these are true statements, but i don't see your point 02:52 < nmz787> so on the makerbot it seems like the actual position of the stage would just be something in between all 4 Z screws, thanks to the wobble arrester springs 02:52 < fenn> oh. yeah, the makerbot is overconstrained 02:53 < nmz787> well I guess we'd need this item on the nut, and leave the Z constrained by the linear rails? 02:53 < fenn> yes 02:53 < nmz787> without this, it would just put undue pressure on the plate/slide bearings 02:54 < nmz787> i think 02:54 < fenn> this is to prevent an eccentric leadscrew from causing uneven friction with the nut, and to prevent side loading of the axis 02:54 < nmz787> right 02:54 < nmz787> ok 02:55 < fenn> so i remember where i stopped working last time, i was trying to "save time" by using existing bearing and stepper motor models 02:55 < fenn> but the existing libraries are more of a pain to use than they're worth 02:58 < nmz787> I remember you linked to something like 'worlds smallest Z stepper' or something a while ago 02:58 < nmz787> something from a CD drive I think 02:59 < nmz787> hacked 03:00 < gnusha> laser_etcher.git: 7d48ccc 03:01 < gnusha> laser_etcher.git: 8548e40 03:06 < fenn> was there supposed to be a commit message? 03:07 < fenn> hm i guess not 03:07 < gnusha> laser_etcher.git: c8f5071 03:08 -!- sylph_mako [~mako@213.195.252.27.dyn.cust.vf.net.nz] has quit [Quit: Leaving] 03:11 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Ping timeout: 240 seconds] 03:13 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap 03:16 < nmz787> fenn, anything that I could help with to further your completion of that model? 03:18 < fenn> find me a nema23 stepper model in openscad 03:19 < fenn> and a 16mm bearing model and 20mm ish thrust bearing 03:19 < nmz787> ok, will try to do when i wake up 03:19 * nmz787 heads toward bed 03:19 < fenn> perhaps i can just use simple cylinders as stand ins 03:19 < fenn> gnight 03:20 -!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Quit: Leaving.] 03:42 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has joined ##hplusroadmap 04:06 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Ping timeout: 276 seconds] 04:14 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 04:20 -!- AdrienG [~ircname@unaffiliated/amphetamine] has joined ##hplusroadmap 04:20 -!- AdrienG is now known as AdrianG 04:24 -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap 04:30 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 276 seconds] 04:32 <@kanzure> oh look. a wild fenn appeared! 04:34 < foucist> quick, get the pokeball out! 04:43 <@kanzure> i am a conscientious objector 04:43 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 04:54 < gnusha> laser_etcher.git: f928f89 added motor and bracket mockup, need accurate dimensions still 04:54 < gnusha> laser_etcher.git: e56df72 clean up coupler dimensions, add hole to bracket for motor shaft 04:54 < gnusha> laser_etcher.git: b196e42 added some leadscrew jazz 04:54 < gnusha> laser_etcher.git: 461f9c6 finish leadscrew 04:55 < fenn> let nmz787 know about http://fennetic.net/irc/leadscrew_assembly.png 04:55 <@kanzure> how do i convince him to use commit messages? 04:56 < fenn> with a large blunt object 04:56 < fenn> such as a trout 04:56 <@kanzure> ... or maybe a pokeball? 04:56 < fenn> not large enough 04:57 < fenn> is there a way to measure distances between points in an stl file? 04:58 <@kanzure> stl is dimensionless but i think blender has some things that pretend to be rulers and measuring sticks? 04:58 < fenn> like, click here, click here, what's the distance 04:58 < fenn> oh right, blender 04:59 < fenn> that nema 23 motor looks really huge in comparison 05:02 <@kanzure> which scad nema motors did you look at? 05:03 < fenn> some stuff in MCAD 05:04 <@kanzure> there's also things like.. http://www.thingiverse.com/thing:3165 05:05 <@kanzure> oh nevermind.. elmom considers his version more complete 05:05 < fenn> cube(size=[bolt_hole_distance+bolt_hole_size+5, 05:05 < fenn> really? the cube is just 5 mm bigger than the bolt holes? 05:06 < fenn> well i guess that works 05:06 < fenn> nema spec says no greater than 57mm 05:07 < fenn> 1.856in+0.195in+5mm = 57.09 mm 05:08 < fenn> blarg why can't everyone just use mm 05:10 < fenn> they also seem to think the motor shaft is 6.36mm while the motors i have are actually 5mm 05:10 < fenn> maybe i should measure that again 05:12 < fenn> so, i'm probably going to go be a hippie and build earthbag domes in the desert 05:13 <@kanzure> is this just a creative way of saying "i'm going to burning man again"? 05:13 < fenn> not that i'm particularly fond of digging in the dirt, but it seems to be a proven design 05:13 < fenn> i'm not going to burning man this year 05:14 < fenn> s/earthbag/superadobe/ 05:15 < fenn> ideally i'd have a robocrane squirt out a concrete shell just like a giant reprap 05:17 < fenn> did dave take my electronics junk too? or is that still in austin? 05:18 <@kanzure> les might still have a few boxes 05:18 <@kanzure> i don't think i had any 05:18 < fenn> you have some sensors from sparkfun and a lego house 05:18 < fenn> and possibly some documents 05:18 <@kanzure> sounds right to me 05:19 < fenn> i should tell les about magnesium 05:19 < fenn> i've never seen the man touch a green piece of food 05:20 <@kanzure> i think his diet is taco bell sauce packets 05:20 < fenn> and whataburger shakes 05:21 < fenn> my new breakfast: 1c hot water, 1T miso, 1t dried seaweed, 1t kelp powder, 1c soba, 1/2 can salmon 05:22 < fenn> it's practically instant if you have a zojirushi 05:22 < fenn> (hot water dispenser) 05:24 < fenn> substitute mackerel for salmon if you're poor 05:29 < gnusha> laser_etcher.git: 67bbbbf i guess i have to use colors inside the module 05:29 < fenn> ah good push works as expected now 05:30 <@kanzure> looks the same? 05:32 < fenn> [remote "origin"] 05:32 < fenn> url = fenn@diyhpl.us:/srv/git/laser_etcher.git 05:32 < fenn> works now with "git push" 05:32 < fenn> before i had to do git push fenn@diyhpl.us:/srv/git/laser_etcher.git master 05:32 <@kanzure> oh i assumed you had that 05:32 <@kanzure> i see 05:32 < fenn> i did have that 05:32 < fenn> but something changed and it works now.. anyway 05:45 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Write error: Broken pipe] 05:47 -!- ParahSailin_ [~parah@69.151.157.115] has joined ##hplusroadmap 05:52 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap 06:00 -!- nsh [~Smedley@wikipedia/nsh] has quit [Ping timeout: 248 seconds] 06:45 -!- nsh [~Smedley@host86-137-188-83.range86-137.btcentralplus.com] has joined ##hplusroadmap 06:45 -!- nsh [~Smedley@host86-137-188-83.range86-137.btcentralplus.com] has quit [Changing host] 06:45 -!- nsh [~Smedley@wikipedia/nsh] has joined ##hplusroadmap 06:51 -!- OldCoder_ [~OldCoder_@c-69-181-140-134.hsd1.ca.comcast.net] has quit [Quit: Leaving] 06:53 -!- OldCoder_ [~OldCoder_@c-69-181-140-134.hsd1.ca.comcast.net] has joined ##hplusroadmap 07:02 -!- minimoose [~minimoose@209.140.69.189] has joined ##hplusroadmap 07:17 -!- minimoose [~minimoose@209.140.69.189] has quit [Read error: Connection reset by peer] 07:52 <@kanzure> "The research you've published in Nucleic Acids Research has contributed to the journal's highest-ever 8.026 Impact Factor from Thomson Reuters' Journal Citation Reports." 08:03 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Ping timeout: 260 seconds] 08:06 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap 08:12 <@kanzure> hmm "Astrobotic today announced a NASA contract to develop technologies for exploring caves on the Moon" so uh, what caves are there? 08:19 < chris_99> can you get high resolution servo controlled robotic arms? 08:29 < jrayhawk> [url "jrayhawk@diyhpl.us:/srv/git/"] pushInsteadOf = git://diyhpl.us/ 08:29 < jrayhawk> pushInsteadOf = git://diyhpl.us/ 08:29 < jrayhawk> ugh, i wonder why that doesn't copy and paste right 08:30 < jrayhawk> [url "jrayhawk@diyhpl.us:/srv/git/"] 08:30 < jrayhawk> pushInsteadOf = git://diyhpl.us/ 08:30 < jrayhawk> there we go 08:30 < jrayhawk> doing something like that might also make your lives slightly easier 08:33 <@kanzure> i never try to push via git:// on diyhpl.us 08:34 < jrayhawk> that would make sense considering it wouldn't work without some special effort 08:35 <@kanzure> is "special effort" a euphemism for "jrayhawk does it" 08:36 < jrayhawk> possibly. i don't remember if git-daemon needs to have its command line changed, but at least the repositories need a special configuration to allow that sort of export. 08:36 <@kanzure> i see 08:36 <@kanzure> (it's not particularly important to me) 08:36 < jrayhawk> core.sharedrepository needs to be 0666, obviously 08:37 < jrayhawk> daemon.uploadarch needs to be set to true, i guess 08:38 < jrayhawk> and daemon.receivepack 08:39 < jrayhawk> anyway, once you have that pushInsteadOf override in place, you can switch remote.origin.url over to git:// 08:39 < jrayhawk> and pulling is magically less irritating 09:07 -!- augur_ [~augur@208.58.5.87] has quit [Remote host closed the connection] 09:09 -!- Falfe [~not@c83-251-81-162.bredband.comhem.se] has joined ##hplusroadmap 09:10 <@kanzure> "As far as I can tell, biohackers are all adventurous young people, incredibly athletic, and they’re all traveling the world." 09:10 <@kanzure> stewart brand has a messed up conception of me :P 09:10 <@kanzure> http://www.wired.com/wiredenterprise/2012/08/ff_stewartbrand/all/ 09:11 <@kanzure> but i do find it funny that kevin kelly and stew are still pimping well 09:11 <@kanzure> i mean, Well 09:22 -!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has joined ##hplusroadmap 09:22 -!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has quit [Changing host] 09:22 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 09:41 -!- jmil [~jmil@hive76/member/jmil] has joined ##hplusroadmap 09:52 -!- augur [~augur@129-2-129-33.wireless.umd.edu] has joined ##hplusroadmap 10:06 -!- rmcl [~rmcl@75.36.45.199] has joined ##hplusroadmap 10:17 -!- rmcl [~rmcl@75.36.45.199] has quit [Quit: rmcl] 10:18 < archels> seems the media is all over everyone recently who is affiliated with the Long Now Foundation. 10:25 <@kanzure> nah.. stew/kk/that gang has had positive media since forever 10:25 <@kanzure> everyone likes the "friendly old guys talking about edge.org stuff" 10:25 <@kanzure> "edge.org: TED but without herpes" 10:27 <@kanzure> oh he did venter recently.. http://edge.org/conversation/what-is-life 10:27 <@kanzure> (video) 10:27 <@kanzure> oh nope. there's a transcript. 10:58 -!- octopine is now known as audy 11:39 -!- mensch [~mensch@c-75-69-228-244.hsd1.ma.comcast.net] has joined ##hplusroadmap 11:43 -!- jmil [~jmil@hive76/member/jmil] has quit [Quit: jmil] 11:49 < archels> By agreement with the publisher, this book is accessible by the search feature, but cannot be browsed. 11:49 < archels> ^ on pubmed. Interesting. 11:50 -!- nsh [~Smedley@wikipedia/nsh] has quit [Read error: Connection timed out] 11:50 <@kanzure> archels: pubmed is negotiating with publishers? 11:51 -!- nsh [~Smedley@host86-137-188-83.range86-137.btcentralplus.com] has joined ##hplusroadmap 11:51 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 11:57 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Write error: Broken pipe] 12:00 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 12:03 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Client Quit] 12:19 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 12:21 -!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 260 seconds] 12:22 -!- AdrienG [~ircname@unaffiliated/amphetamine] has joined ##hplusroadmap 12:26 -!- AdrianG [~ircname@unaffiliated/amphetamine] has quit [Ping timeout: 240 seconds] 12:31 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 12:52 -!- nmz787 [~Nathan@173-114-252-246.pools.spcsdns.net] has joined ##hplusroadmap 12:56 -!- Jaakko96 [~Jaakko@94-194-89-130.zone8.bethere.co.uk] has joined ##hplusroadmap 12:57 <@kanzure> nmz787: how's meredith? 12:57 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Ping timeout: 276 seconds] 13:05 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 13:10 -!- drazak__ [~ahdfadkfa@69.162.134.185] has joined ##hplusroadmap 13:10 -!- drazak__ [~ahdfadkfa@69.162.134.185] has quit [Client Quit] 13:15 -!- Jaakko96 [~Jaakko@94-194-89-130.zone8.bethere.co.uk] has quit [Quit: Nettalk6 - www.ntalk.de] 13:16 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Ping timeout: 276 seconds] 13:17 -!- marainein [~net@2001:388:608c:6cb5:d06:5bd6:a36f:c92] has quit [Ping timeout: 245 seconds] 13:19 -!- nmz787 [~Nathan@173-114-252-246.pools.spcsdns.net] has quit [Quit: Leaving.] 13:30 -!- marainein [~net@2001:388:608c:6cb5:c85a:fbcc:e87f:e157] has joined ##hplusroadmap 13:32 -!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 13:33 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 244 seconds] 13:38 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 244 seconds] 13:40 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 13:41 <@kanzure> http://options-vs-salary.com/ is a neat tool. 13:44 < chris_99> fancy 13:51 < brownies> not a very good UX 13:51 < brownies> but i guess it's nice of them to do the multiplication for you 13:52 -!- AdrienG [~ircname@unaffiliated/amphetamine] has left ##hplusroadmap [] 14:01 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 14:15 -!- Juul [~Juul@208.87.217.74] has joined ##hplusroadmap 14:18 < chris_99> http://neurophone.wordpress.com/2012/08/05/make-a-diy-flanagan-neurophone-with-a-tl494/# 14:22 -!- loanshark [~loanshark@ip72-218-133-8.hr.hr.cox.net] has joined ##hplusroadmap 14:22 <@kanzure> hi loanshark 14:23 < loanshark> hey again 14:23 <@kanzure> so you want to get into bioengineering 14:23 <@kanzure> try this? http://diyhpl.us/wiki/diybio/faq 14:23 < loanshark> yes! 14:23 < loanshark> thankyou 14:23 < loanshark> i have one question 14:24 < loanshark> its about obtaining DNA because i watched that video about making antidepressant yogurt 14:24 <@kanzure> that guy didn't really make antidepressant yogurt 14:24 <@kanzure> he was bullshitting you 14:24 < loanshark> where do you order the genes? 14:24 < loanshark> o.o 14:24 < loanshark> awww 14:24 < yashgaroth> there's no prozac gene 14:24 < loanshark> i thought that was cool 14:25 <@kanzure> as far as i know there's no prozac gene or prozac metabolic pathway 14:25 < yashgaroth> plus it's got a bunch of fluorines on there which is pretty much impossible to do enzymatically 14:26 < EnLilaSko> loanshark: The video was pretty much showing you can do shittons of stuff at home, but the prozac part was just a joke/troll 14:26 < yashgaroth> I wish he 14:26 < yashgaroth> 'd made that last part more obvious 14:26 < loanshark> xD 14:26 <@kanzure> yashgaroth: i should just make wild claims like that too 14:27 <@kanzure> yashgaroth: "i have created yogurt that turns me into a hermit!" 14:27 <@kanzure> wait maybe that's not sufficiently impressive 14:27 < EnLilaSko> lol 14:27 < loanshark> :3 14:27 < yashgaroth> oh I made a talking mouse 14:27 -!- Juul [~Juul@208.87.217.74] has quit [Ping timeout: 260 seconds] 14:27 < loanshark> anyways, where do you get the DNA? 14:27 < loanshark> o.o 14:27 < EnLilaSko> "I make a yogurt curing AIDS" 14:27 < EnLilaSko> All the newspaper would write about it 14:27 < yashgaroth> what DNA 14:27 < EnLilaSko> He gives you a site loanshark. 14:27 <@kanzure> loanshark: you can get dna from yourself 14:27 < EnLilaSko> The bacterias 14:28 < yashgaroth> I poured this yogurt into a dish of cancer cells and they all died, money please 14:28 < yashgaroth> why is no one funding this?!?! 14:28 < loanshark> EnLilaSko: i still don't see how to actually get the DNA, there is no "buy now" xD 14:28 < ParahSailin> i made yogurt that was an AGI, but i got too hungry and ate it 14:28 <@kanzure> loanshark: there is "buy now", you are lying 14:28 < EnLilaSko> Ah, then I have no clue 14:28 < yashgaroth> origene.com 14:28 < loanshark> :o 14:28 <@kanzure> or idtdna.com is a popular choice 14:29 < EnLilaSko> I usually buy cooler people genes so I maybe become cool 14:29 < loanshark> so 14:29 < loanshark> its $220 14:29 < loanshark> xD 14:29 < loanshark> DIY bioengineering is expensive! 14:30 < yashgaroth> what gene are you looking at 14:30 < loanshark> idk 14:30 < loanshark> :3 14:31 * loanshark is a newb 14:31 <@kanzure> idk isn't a really interesting gene 14:31 <@kanzure> why are you looking at idk 14:31 < loanshark> o.o 14:31 < yashgaroth> I saw a paper about idk3 recently, has a novel protease function 14:32 < loanshark> ok 14:32 < loanshark> I'm gonna read that think kanzure gave me 14:32 < loanshark> because i know zilch about genes and stuff 14:32 <@kanzure> also read everything here http://diyhpl.us/~bryan/papers2/bio/ 14:32 <@kanzure> and http://diyhpl.us/wiki/diybio/faq 14:32 < loanshark> ;_; 14:32 < loanshark> can i just buy a book 14:33 < loanshark> with everything in it 14:33 < yashgaroth> I recommend molecular biology of the cell, or molecular biology of the gene 14:33 <@kanzure> no 14:33 < loanshark> like my big encyclopedia of sailing 14:33 < yashgaroth> but they don't really cover actual genetic engineering 14:33 < loanshark> what have you guys done with genes? 14:33 <@kanzure> also, that question is answered in the faq 14:33 <@kanzure> so is that one 14:34 < yashgaroth> bro I do stuff with genes erry day 14:34 < loanshark> so many windows open now my head hurts x_x 14:34 < EnLilaSko> I like, live with mine 14:35 < yashgaroth> though you should know, people have spent more hours writing news articles about biohacking than actually biohacking 14:35 <@kanzure> loanshark: you can never have enough tabs http://heybryan.org/shots/2008-01-15-tabs.png 14:35 <@kanzure> https://addons.mozilla.org/en-US/firefox/addon/toomanytabs-saves-your-memory/ 14:35 < loanshark> xD 14:36 < EnLilaSko> damn, lol 14:40 < loanshark> :3 learning is fun 14:40 < loanshark> are there any easy, cheap projects i can do to get started? 14:41 <@kanzure> http://diyhpl.us/wiki/diybio/faq/kits 14:41 < yashgaroth> define easy/cheap 14:41 < loanshark> umm 14:41 < loanshark> under $100 14:41 <@kanzure> you can do the shotglass dna extraction project 14:41 <@kanzure> but it's more of a parlor trick and completely useless 14:42 <@kanzure> unless you happen to need to purify human dna for some project 14:42 < loanshark> what do you do with the DNA that has been extracted? 14:42 <@kanzure> you eat it 14:42 <@kanzure> or just stare at it 14:42 < TheEmpath> om nom life books 14:42 < yashgaroth> you get drunk in the process, that's a bonus 14:42 < loanshark> you can't do anything at all with it? 14:43 <@kanzure> loanshark: biology is not cheap.. you shouldn't put price limits like that on it 14:43 <@kanzure> there's a $25 lightbulb pcr machine design that russell put up 14:43 <@kanzure> and staceyk put up a $85 arduino-based pcr machine too, but you need to buy >$100 of pcr master mix (which you can sometimes get a free sample for..) 14:44 < yashgaroth> but if you want to see the result of the pcr, you'll need a gelbox and supplies for that, oh and a centrifuge 14:44 < loanshark> whats a pcr? 14:45 < yashgaroth> http://www.ncbi.nlm.nih.gov/books/NBK21054/ 14:46 < gnusha> diyhpluswiki.git: 22b779a add a link to the kits page from diybio/faq.mdwn 14:46 < loanshark> http://www.amazon.com/Biopunk-Scientists-Hack-Software-Life/dp/1617230022/ref=sr_1_1?ie=UTF8&qid=1345585572&sr=8-1&keywords=biohack 14:46 < loanshark> maybe? 14:46 < yashgaroth> doesn't actually have any protocols, but sure 14:46 < yashgaroth> please see [14:35:18] .:yashgaroth:. though you should know, people have spent more hours writing news articles about biohacking than actually biohacking 14:46 < loanshark> akk 14:46 < loanshark> nvm 14:47 < gnusha> diyhpluswiki.git: 0a48059 add molecular biology of the cell on nih to book list 14:47 <@kanzure> loanshark: that book is just about me and my friends 14:47 <@kanzure> i mean, it doesn't actually tell you how to do things 14:47 < yashgaroth> genetic engineering isn't its own field, it's a very specialized application of biology 14:47 < loanshark> >.< 14:48 <@kanzure> loanshark: http://www.ncbi.nlm.nih.gov/books/NBK21054/ is a good intro book, but it wont teach you techniques 14:48 <@kanzure> oh wait, it seems to have some chapters on "methods" in part 3 14:48 <@kanzure> yashgaroth: would you believe me if i told you i haven't actually read that book? 14:48 < yashgaroth> no, but it's questionable whether knowing how to run PCR is worthwhile if you don't know what PCR is 14:48 < yashgaroth> sure kanzure 14:49 < yashgaroth> but you know what pcr is so it's a moot point 14:49 < loanshark> oki 14:49 < loanshark> getting the 5th edition 14:49 <@kanzure> huh? why the 5th? 14:49 <@kanzure> it's right here: http://www.ncbi.nlm.nih.gov/books/NBK21054/ 14:49 <@kanzure> boom you have it 14:50 < yashgaroth> nothing you'll need to know outside of a bio PhD program has been added between editions, but feel free 14:50 <@kanzure> yashgaroth: maybe there's a cut-away phd hidden between pages 100 and page 101 14:50 < loanshark> i was just downloading it o.o 14:50 < yashgaroth> include 8 years of your life in the mail-away envelope 14:51 < loanshark> its $159 dollars! 14:51 <@kanzure> yes, because textbooks are evil 14:51 <@kanzure> why are you paying for it? 14:51 < yashgaroth> oh we're the last people who'll tell you to pay for it 14:51 < loanshark> im not 14:51 < loanshark> but still 14:51 < loanshark> thats a lot 14:52 <@kanzure> yashgaroth: oh that book isn't readable on the nih site 14:52 < yashgaroth> lolwut 14:52 <@kanzure> as far as i can tell? 14:52 < yashgaroth> oh haha well fuck 'em then 14:52 < yashgaroth> yeah you're right, just noticed the header 14:53 < yashgaroth> "By agreement with the publisher, this book is accessible by thepiratebay, but cannot be browsed." 14:53 < loanshark> :3 14:53 -!- jmil [~jmil@hive76/member/jmil] has joined ##hplusroadmap 14:53 < loanshark> i need to charge my kindle 14:53 < loanshark> any other books? 14:54 < yashgaroth> lehninger's biochemistry 14:54 < yashgaroth> molecular biology of the gene 14:54 < yashgaroth> human molecular genetics 14:54 <@kanzure> i liked campbell biology but that was probably because the alternative (at the time) was holt biology 14:55 < yashgaroth> I forget which one we had in school, fuck food chains 14:55 <@kanzure> "do you know what a CONSUMER is?" 14:56 < loanshark> do i need human molecular genetics? 14:56 <@kanzure> depends on what you are doing or why 14:56 < loanshark> i just like biology 14:56 < loanshark> i have no idea what you can/can't do with bioengineering 14:57 < loanshark> i thought you could make prozac yogurt :3 14:57 <@kanzure> why did you think that 14:57 < yashgaroth> that fucking douchebag and his shitass video 14:57 < loanshark> because 14:58 < loanshark> i searched 'antidepressant' in the registary 14:58 < loanshark> and it came up with some stuff 14:58 <@kanzure> what is registary? 14:58 -!- augur [~augur@129-2-129-33.wireless.umd.edu] has quit [Remote host closed the connection] 14:58 < loanshark> registry** -.- 14:58 <@kanzure> which registry 14:58 < loanshark> partsregistry.org 14:59 <@kanzure> ok why are you using that site 14:59 < yashgaroth> I see a gene activated by a component of st john's wort 15:00 <@kanzure> http://partsregistry.org/Part:BBa_K364325:Experience 15:00 <@kanzure> i see a unicode/utf8/charset error 15:01 < yashgaroth> oh god I was browsing igem team wikis last night, the UGeorgia team had extremely loud unstoppable gangsta rap on their page 15:02 <@kanzure> igem: the official myspace of synthetic biology 15:03 <@kanzure> bleh 15:03 < yashgaroth> anyway loanshark there's very little you can do at the moment outside of a legitimate lab, especially if you have no bio knowledge or experience 15:04 <@kanzure> you will have to acquire equipment 15:04 -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has quit [Quit: - nbs-irc 2.39 - www.nbs-irc.net -] 15:04 <@kanzure> and reagents (consumables) 15:05 < loanshark> but what can you do if you have a small home lab with a PCR and centrifuge and other DIY equipment 15:05 < yashgaroth> you can spend your time finding a company that will actually sell reagents to an individual 15:06 < yashgaroth> you could conceivably sequence parts of your own genome 15:06 < yashgaroth> with an incubator you can make some glowing bacteria 15:06 <@kanzure> most of the time what happens is that a newbie will pick a huge project 15:06 <@kanzure> and then spend a few months acquiring equipment and planning it out 15:06 <@kanzure> and then realize how impractical it is, and then scale back and pick something more doable 15:07 <@kanzure> with the equipment already acquired 15:07 < loanshark> can anyone suggest an feasible project? 15:08 < yashgaroth> as in a project where you follow someone else's protocols, or original research? 15:09 < loanshark> follow someone else's protocols 15:09 < loanshark> :3 15:09 <@kanzure> ok well pick something: http://protocol-online.org/ 15:10 < yashgaroth> there's one where you make bacteria smell like bananas, dunno if they made the gene publicly available 15:11 < yashgaroth> sadly it's not yet like computer hacking where the limit is your imagination and expertise 15:12 < loanshark> :3 15:13 -!- eudoxia [~eudoxia@r186-54-230-60.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 15:14 < eudoxia> kanzure, which open source cad program do you see as having the most potential as a component of SKDB 15:14 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 15:14 < eudoxia> or just the best FOSS CAD program right now 15:14 <@kanzure> eudoxia: brlcad is the best-written cad program 15:14 <@kanzure> unfortunately it doesn't support nurbs quite yet 15:15 <@kanzure> i mean, it does in a few places, but not in the ways that matter 15:15 <@kanzure> but it's definitely superior to openscad 15:15 < eudoxia> i was just going to ask you about that one 15:16 <@kanzure> i don't recommend openscad at all 15:16 <@kanzure> but http://diyhpl.us/laser_etcher/laser_etcher models are written with it 15:16 < eudoxia> why not? 15:16 <@kanzure> there's no particular reason for its custom language.. that's just silly 15:17 <@kanzure> variables are a hack or unsupported 15:17 <@kanzure> it's just a tessellation engine really, which you get for free with brlcad or blender, which makes it really not that much of a cad tool.. 15:17 < eudoxia> what is the standard language for programmatic cad then? 15:17 <@kanzure> autolisp 15:18 <@kanzure> implicitcad is sorta neat if you're into haskell 15:18 < loanshark> so 15:18 < loanshark> :/ 15:18 < loanshark> could i for example 15:18 <@kanzure> eudoxia: but i'm a big sucker for python cad 15:18 <@kanzure> eudoxia: so stuff like pythonocc (even though opencascade/oce is shit) 15:19 <@kanzure> i mean, opencascade is at least a real cad kernel 15:19 <@kanzure> the problem is that its quality is very low 15:19 < eudoxia> can BRLCAD use autolisp? 15:19 < loanshark> take the gene that makes a type of algae glow and put it in another organism? 15:19 < loanshark> 15:19 <@kanzure> just none of the other cad offerings really meet that definition ("real cad kernel") except some of the commercial stuff (acis, parasolid, whatever) 15:19 <@kanzure> eudoxia: brlcad has a number of separate libraries that you can wrap into whatever language you want 15:20 <@kanzure> loanshark: no we don't do glowing 15:20 < eudoxia> that's good 15:20 < loanshark> soo 15:20 < loanshark> :/ 15:20 < loanshark> what can you do? 15:20 <@kanzure> loanshark: maybe you should learn a little first, and then see if anything gets you interested 15:20 <@kanzure> you can do anything that's possible 15:21 < loanshark> I'm just trying to find some applications 15:22 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 260 seconds] 15:24 < loanshark> im really disappointed about the antidepressant thing 15:24 < eudoxia> kanzure: apparently some people have written interfaces to BRLCAD for python, why not use those if openCASCADE is shit? 15:24 < loanshark> i thought you made the bacteria produce some chemical that does the same thing that prozac does 15:25 < loanshark> the only example i can think of is how chemically synthetic THC does the same thing as regular THC 15:25 -!- tellurium [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has joined ##hplusroadmap 15:25 <@kanzure> eudoxia: opencascade can do nurbs cad, brlcad can't (because opennurbs is made by rhino, and rhino keeps the good parts proprietary) 15:25 <@kanzure> eudoxia: i haven't seen the brlcad python wrapper.. can you link me? 15:26 < eudoxia> http://forums.reprap.org/read.php?12,14558,15121 15:26 <@kanzure> lolz released on a forum.. yeah uh 15:26 <@kanzure> "That said, is there a reason you aren't using something like ctypes and libwdb for this? If you're just doing compile-style generation of geometry, that's exactly what libwdb is for, and that'd keep you from needing to do anything with TCL or mged at all, and make it easier to add libged's functionality when it gets that far. Looking at ctypes, that should be close to trivial, and certainly easier than mucking about generating input for mged" 15:27 <@kanzure> hmm so he's not using ctypes or cython? 15:27 <@kanzure> yeah so i don't think this is a good idea 15:27 <@kanzure> generating input for mged is just as bad as pyopenscadpy 15:28 <@kanzure> (i forget which one; pyopenscad or openscadpy generates input for openscad; the other one is openscad compiled with Python.h) 15:29 <@kanzure> eudoxia: i'd even take a swig-generated interface in python over that 15:30 <@kanzure> (that's what pythonocc was for opencascade, just some swig wrapper around opencascade) 15:31 < eudoxia> so BRLCAD is good but can't do nurbs and opencascade is shit but can do nurbs 15:31 < eudoxia> how complicated 15:32 <@kanzure> it gets much more complicated :) 15:32 <@kanzure> like go look at the opencascade source code 15:32 <@kanzure> http://github.com/kanzure/oce 15:32 <@kanzure> and compare it to brlcad (which is heaven in comparison) 15:34 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 15:35 < loanshark> kanzure: is there nothing cool you can do with bioengineering in a home lab? 15:36 <@kanzure> there's tons of cool stuff. you can do genetic engineering. 15:37 < loanshark> what can you do with genetic engineering in a home lab? 15:42 <@kanzure> http://diyhpl.us/wiki/diybio/faq/projects 15:44 < loanshark> cool thanks :) 15:44 < loanshark> i didn't see that 15:44 -!- eudoxia [~eudoxia@r186-54-230-60.dialup.adsl.anteldata.net.uy] has quit [Quit: quit] 15:48 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 260 seconds] 15:52 < loanshark> so 15:52 < loanshark> you can just grow cultures or build lab equipment? 15:53 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 15:55 < loanshark> kanzure: what can you do with genetic engineering in a home lab if you can't make SSRI yogurt or organisms that glow 16:00 < ParahSailin> not much actually 16:00 < loanshark> http://partsregistry.org/Part:BBa_K364325:Experience 16:01 < loanshark> that won't make the bacteria make hyperforin? 16:01 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 16:01 < loanshark> hypericin** 16:04 <@kanzure> you can make organisms glow, but i wouldn't be very supportive 16:05 < loanshark> why not? 16:05 <@kanzure> that page says that PXR is activated by hyperforin..... why do you think this gene product would make hyperforin?? 16:05 < loanshark> i meant hyericin 16:05 < loanshark> which is the SSRI 16:06 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 245 seconds] 16:08 -!- OldCoder [~OldCoder@c-69-181-140-134.hsd1.ca.comcast.net] has joined ##hplusroadmap 16:08 < OldCoder> kanzure, loanshark Hi 16:08 < OldCoder> kanzure, I lost this channel 16:08 < OldCoder> Found it again 16:09 < loanshark> helllo 16:09 < OldCoder> loanshark, you were looking for a DIY project 16:10 < loanshark> http://www.mnn.com/green-tech/research-innovations/photos/12-bizarre-examples-of-genetic-engineering/glow-in-the-dark-c 16:10 < loanshark> this stuff is cool 16:10 < OldCoder> Reviewing 16:10 < loanshark> glow cats! 16:11 < OldCoder> I've read about this 16:11 < OldCoder> But I wouldn't try this at home! :-) 16:11 < loanshark> why not? 16:12 < OldCoder> Gengineering kitties? 16:12 * OldCoder reads 16:12 < OldCoder> You don't have the equipment or training needed to do something at this level. Glowing microorganisms are different. 16:12 < OldCoder> You could make a bottle of pond water glow. 16:13 < loanshark> could totally set up and operate on kitties 16:13 < loanshark> ;3 16:13 * OldCoder shrugs 16:13 < loanshark> what i mean is that that is cool, 16:14 < OldCoder> It is 16:14 < OldCoder> Glowing tomatoes are useful 16:14 < loanshark> like interesting things that spark my interest in bioengineering and motivate me to learn about it 16:14 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 16:14 < OldCoder> Good 16:14 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Remote host closed the connection] 16:14 <@kanzure> loanshark: no, glowing is NOT cool 16:14 <@kanzure> fuck 16:14 <@kanzure> fuuuck 16:14 < OldCoder> ? 16:14 < OldCoder> Not glowing kitties 16:14 < OldCoder> Maybe glowing pond water 16:14 < OldCoder> kanzure, hi 16:14 <@kanzure> it's not even really that interesting: it's just glowing under fluorescent light 16:14 < loanshark> getting dan from a strawberry with a shot glass for no reason what so ever does not sound very interesting 16:15 <@kanzure> it's not 16:15 < OldCoder> Conceptually 16:15 < chris_99> kanzure, what stuff have you done that's cool relating to this that's cool? 16:15 < loanshark> :3 cool stuff 16:15 < chris_99> er, that was poorly worded 16:15 <@kanzure> chris_99: i did some published research on transcriptional logic gates under pcr 16:15 < loanshark> pollution-fighting plants! 16:15 < loanshark> thats cool 16:15 < OldCoder> chris_99, I liked the wording 16:15 < chris_99> link kanzure? 16:16 <@kanzure> chris_99: i don't have a link handy 16:16 <@kanzure> i don't know if it counts as cool though 16:16 < chris_99> is it on scholar? 16:16 <@kanzure> probably 16:16 <@kanzure> but i'm not cited 16:16 <@kanzure> because i was the disposable undergrad 16:17 < loanshark> logic gates are uncool 16:17 < OldCoder> That's always fun 16:17 < loanshark> :3 16:17 < chris_99> remember the title or other authors? 16:17 <@kanzure> chris_99: yes 16:17 <@kanzure> but i don't think it matters? 16:17 < OldCoder> kanzure, do you have a Twitter presence? 16:18 <@kanzure> OldCoder: sadly yes http://heybryan.org/ lists it 16:18 < OldCoder> Sadly :-) 16:18 < loanshark> how about a cabbage that makes scorpion venom as a sort of self-pesticide, killing caterpillars that bite it, but not so much that it is harmful to humans 16:18 < loanshark> :3 16:18 < loanshark> that is very cool 16:18 <@kanzure> loanshark: how about you just pick a project you like, instead of hoping someone gives you a ready-made package 16:18 < loanshark> i was just asking for ideas and no one had any 16:18 <@kanzure> loanshark: ok so first you should go figure out how to make venom 16:19 < OldCoder> kanzure, PM if all right 16:19 <@kanzure> and then you will see that the expression system probably doesn't work in cabbage 16:19 < loanshark> and someone said there was not much you can do with bio-engineering 16:19 <@kanzure> loanshark: you can do all sorts of things, but that doesn't mean they are easy 16:19 < OldCoder> kanzure, I am already following you. You should tweet more. 16:19 <@kanzure> no i shouldn't 16:19 < loanshark> http://www.nature.com/cr/journal/v12/n2/full/7290120a.html 16:19 <@kanzure> don't encourage me 16:19 < OldCoder> kanzure, do it 16:19 < loanshark> venomous cabbage already exists 16:19 < OldCoder> dooooooooooo eeeeeeeeeeeeeeeeeet 16:19 < OldCoder> :-) 16:20 <@kanzure> loanshark: that was in ecoli 16:20 <@kanzure> loanshark: if you can replicate that paper's work, then you could start to consider doing it in cabbage 16:20 < OldCoder> kanzure, I'll be here a bit. PM when you can. 16:20 <@kanzure> OldCoder: you may pm me 16:20 < OldCoder> I did 16:20 < OldCoder> Tried again 16:20 <@kanzure> ok. 16:21 < loanshark> :3 16:21 < loanshark> how about just making things glow 16:21 < loanshark> like worms 16:21 <@kanzure> loanshark: that looks like a chemical synthesis, not a recombinant protein 16:22 < chris_99> anyone think of something funky that requires a vacuum? 16:22 < loanshark> :3 16:22 <@kanzure> oh nevermind "AaIT is an excitatory long-chain toxin specific to insects and is composed of 70 amino acids with four disulfide bridges" 16:22 <@kanzure> so it looks like this toxin is just an amino acid chain 16:23 < brownies> uh... what? something funky that requires a vacuum? 16:23 < chris_99> yeah heh, i'm getting a vacuum pump for something else, but was wondering if theres anything else i could use it for 16:24 <@kanzure> um well 16:24 <@kanzure> i'm sure my mom has a 16:24 <@kanzure> i can't do this anymore 16:24 < brownies> superconducting? cold temperatures in general? fabricating microchips? 16:24 <@kanzure> oh 16:24 < brownies> basically... everything? 16:24 <@kanzure> is this a 10^-9 torr vacuum? or more? 16:24 < brownies> just... Science™ 16:24 < brownies> mm, good question 16:25 < loanshark> could i inject genes into worm eggs? 16:25 < chris_99> 0 - 30" Hg apparently 16:25 <@kanzure> yep.. but you would have to follow a protocol if you want genetic manipulation of worm eggs to work 16:25 < loanshark> what is a protocol? 16:26 <@kanzure> black magic 16:26 < loanshark> published results of someones research? 16:26 <@kanzure> no 16:26 <@kanzure> http://protocol-online.org/ 16:26 <@kanzure> they are list of steps to do things 16:26 <@kanzure> but usually they are highly specific 16:26 < loanshark> so like a tutorial 16:26 <@kanzure> like incubate at certain temperatures, use certain amounts of whatever reagents and liquids, etc. 16:26 <@kanzure> no 16:26 < loanshark> recepies? 16:26 <@kanzure> really bad recipes 16:26 < loanshark> ok 16:27 < loanshark> will there really be a form egg gene injection protocol? 16:28 <@kanzure> there might be, if someone has done it before 16:28 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 16:28 <@kanzure> but if not, you will have to spend months/years figuring it out based on related lab techniques and testing 16:28 < loanshark> :3 16:28 < loanshark> ok 16:29 < loanshark> because i might just be killing them 16:29 < loanshark> XD 16:29 <@kanzure> you will definitely screw up and kill things 16:29 < loanshark> hardcore 16:29 < loanshark> http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html 16:30 < loanshark> the best relating protocol i found - microinjection 16:31 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Remote host closed the connection] 16:32 < loanshark> theoretically, if you get the single fertilized cell 16:32 <@kanzure> conditions for injecting plasmids into embryos are fairly non-trivial, it varies dramatically by species 16:32 < loanshark> you can do that electrothingy to open the barriers and then centrifuge cells with glow-in-the dark gene 16:33 <@kanzure> you are a master of technobabble 16:33 < loanshark> ^^ thanks 16:33 < OldCoder> write stories 16:33 < loanshark> stories? 16:33 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 16:37 -!- Falfe [~not@c83-251-81-162.bredband.comhem.se] has quit [Ping timeout: 244 seconds] 16:38 < loanshark> Kanzure: if you had the gene that makes luciferase then put that in the worms they would be like fireflies? 16:38 <@kanzure> read the diybio archives about that.. short answer is no 16:38 <@kanzure> https://google.com/search?q=diybio+luciferase 16:42 < loanshark> so apparently fluorescence is way easier than bioluminescence 16:44 < loanshark> awww 16:44 < loanshark> synthesizing genes is expensive :( 16:44 <@kanzure> yes dna costs a bit much 16:45 <@kanzure> yashgaroth: maybe diybio is just a clever marketing stunt for carolina's gfp kits 16:45 < loanshark> but you can just go out and capture some fireflies 16:46 < yashgaroth> it's sad how true that could be kanz 16:50 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 268 seconds] 16:54 < loanshark> how would you collect fluorescent protein genes from nature? 16:55 <@kanzure> you would buy some dead squid and pcr it out of their genome with some primers 16:56 < loanshark> ok 16:56 < loanshark> :3 16:56 < loanshark> see now this is cool :3 16:56 < yashgaroth> well they probably have introns, in which case I'd recommend reverse-transcriptase pcr 16:56 <@kanzure> no it's not.. it's just fluorescence dude 16:57 < loanshark> but 16:57 < loanshark> your engineering with biology.. building cool things 16:57 < loanshark> changing them the way you want them 16:57 < loanshark> :3 16:58 < loanshark> like lego, but with living things 16:58 < loanshark> now thats cool 16:59 <@kanzure> it's not like lego at all 16:59 < loanshark> its cooler than lego 17:03 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 17:12 < OldCoder> Living Lego 17:12 < OldCoder> Hmm 17:12 <@kanzure> Juul: do you have a standard disclaimer for "biobricks don't usually work" 17:13 < loanshark> are primers expensive? 17:14 <@kanzure> they are synthetic dna 17:14 < loanshark> :/ 17:14 <@kanzure> so they cost the same, they are just usually short - about 20 bp to 40 bp or longer ish 17:14 < loanshark> arg! 17:14 < yashgaroth> a few dollars each 17:14 < loanshark> oh? 17:15 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 268 seconds] 17:16 < yashgaroth> well more than a few, but not "same" like several hundred dollars 17:16 <@kanzure> true 17:17 < loanshark> 4.90 per primer 17:17 < loanshark> :D 17:28 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 17:29 < loanshark> http://www.operon.com/products/pcr-primers/pcr-primer-overview.aspx?id=pcr 17:29 < loanshark> Kanzure $4.90 a primer sounds very affordable 17:30 <@kanzure> and what are you planning to do with these primers 17:30 < loanshark> get the luminescent gene 17:31 <@kanzure> why not just use the kit from carolina 17:31 <@kanzure> http://diyhpl.us/wiki/diybio/faq/kit 17:31 <@kanzure> oops 17:31 <@kanzure> http://diyhpl.us/wiki/diybio/faq/kits 17:32 < loanshark> how much is it? 17:32 <@kanzure> cheaper than you building your own plasmid system and consuming all those reagents in test 17:34 < loanshark> :/ 17:34 < loanshark> really? 17:34 <@kanzure> well, do the math and prove me wrong 17:35 < loanshark> idk what it takes to build a plasmid system 17:35 <@kanzure> go find out 17:35 < loanshark> but wikipedia tells me it is a DNA molecule separate from the chromosomal DNA 17:39 <@kanzure> correct 17:39 <@kanzure> this is one of the gene expression methods 17:43 < loanshark> aaah 17:43 < loanshark> this stuff is intense 17:43 < loanshark> :3 17:43 < loanshark> so 17:44 <@kanzure> loanshark: feel free to edit the diybio faq with your findings 17:49 < loanshark> so you get the DNA 17:49 < loanshark> then use the DNA to make DNA plasmids? 17:50 -!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has joined ##hplusroadmap 17:50 < gnusha> diyhpluswiki.git: ccaa470 increase instructions for editing the faq 17:52 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 17:58 < tashoutang> excuse me, last time someone give me a clue to search some chemical compound those could be useful after extraction, and can this Mister/Miss give me that clue again? 18:01 < gnusha> diyhpluswiki.git: 18:01 <@kanzure> jrayhawk: remote: 2012/08/21 18:01:03 socat[29849] E write(3, 0x9da1388, 56): Broken pipe 18:03 < jrayhawk> curious 18:04 < gnusha> honk honk 18:04 < jrayhawk> well, beats me 18:06 < gnusha> diyhpluswiki.git: 72e834f add a page about the irc channel 18:07 <@kanzure> hmm the formatting is wacky: http://diyhpl.us/wiki/hplusroadmap#irc 18:08 <@kanzure> i tried \#\#[[hplusroadmap]] but instead i get that^ 18:11 <@kanzure> on the other page \#\#[[hplusroadmap]] works.. maybe this is a bug about linking to your own page 18:14 < gnusha> diyhpluswiki.git: 97a74d9 linkify the irc clients list 18:16 < gnusha> diyhpluswiki.git: c2c7913 fix ##hplusroadmap formatting 18:16 <@kanzure> huh, no, the formatting is still very wrong 18:18 < brownies> the logs are public? oh man 18:19 < OldCoder> brownies, I didn't know you were here 18:19 < brownies> OldCoder: i didn't know you were here! 18:19 < tashoutang> @K: did you see my question? 18:19 < OldCoder> I lost the channel 18:20 < tashoutang> excuse me to bother you @K 18:20 < brownies> < kanzure> but then why did I start off as a moron? 18:20 < brownies> doesn't take long to find things and take them out context, when you have 4 years of logs 18:23 <@kanzure> tashoutang: yes i saw your question. there are many chemical compounds. i'm not sure if i told you any. 18:24 < gnusha> diyhpluswiki.git: c9058b7 better instructions for editing the wiki 18:24 < tashoutang> oooo, and in private I ask you that are u talking about the website setting with html language 18:25 <@kanzure> ah i see. 18:25 <@kanzure> well, no, this is not quite html 18:25 <@kanzure> it's markdown being converted to html 18:25 < tashoutang> haha I am still not very familiar with mirc 18:25 < tashoutang> OK 18:26 <@kanzure> no it worked, but i am in too many irc channels and do not see private notifications easily :) 18:26 < tashoutang> I see 18:26 < tashoutang> thanks!:) 18:26 < brownies> kanzure: what client do you use? 18:27 < gnusha> diyhpluswiki.git: 775970d describe the wiki in the intro page 18:27 <@kanzure> brownies: irssi 18:28 -!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 268 seconds] 18:31 < gnusha> diyhpluswiki.git: 8caf394 list out some interesting pages 18:37 < tashoutang> on DIYbio someone said they want to start a DIYbio project whitch include each individual around the world, and welcome every one to offer its idea, and the one who propose this poster said he prefer to plane a project to build a creature and put it on the mars. 18:37 < gnusha> diyhpluswiki.git: 8d9a3d1 move text files to wiki files in transcripts/startup-science-2012 18:37 <@kanzure> tashoutang: yes that was funny 18:38 < tashoutang> I know that scientist had built artificial micoplasma...\ 18:38 <@kanzure> brownies: these might be slightly readable now.. http://diyhpl.us/wiki/transcripts/startup-science-2012/ 18:38 <@kanzure> oh crap, nope 18:38 < tashoutang> why they just start to simulate the surviving ability from that creature first? 18:39 < gnusha> diyhpluswiki.git: b089f36 convert transcripts/startup-science-2012 to markdown 18:39 <@kanzure> brownies: ok try now. 18:41 * brownies looks 18:41 < brownies> kanzure: much better. the whole site is still too wide. 18:41 < brownies> jam the whole thing into a 940px div. that would be a huge step forward. 18:41 <@kanzure> can you pop open chrome inspector and suggest a -- 18:42 <@kanzure> ok 940px div.. will try that 18:42 <@kanzure> wait do i care 18:44 < gnusha> diyhpluswiki.git: 1f31d45 restrict the body element to 940px 18:44 <@kanzure> brownies: how about now? 18:44 < brownies> kanzure: much better. 18:44 <@kanzure> blah the title stuff isn't in a block div http://diyhpl.us/wiki/transcripts/startup-science-2012/carlo-quinonez/ 18:45 < brownies> kanzure: just throw that search bar next to the links 19:08 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 19:17 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Quit: Leaving] 19:18 -!- roksprok [~Zac@50-0-91-31.dsl.dynamic.sonic.net] has joined ##hplusroadmap 19:19 -!- roksprok [~Zac@50-0-91-31.dsl.dynamic.sonic.net] has quit [Client Quit] 20:06 -!- ivan`_ [~ivan@unaffiliated/ivan/x-000001] has quit [Ping timeout: 276 seconds] 20:07 -!- ivan` [~ivan@unaffiliated/ivan/x-000001] has joined ##hplusroadmap 20:26 -!- ivan` [~ivan@unaffiliated/ivan/x-000001] has quit [Ping timeout: 260 seconds] 20:29 -!- ivan` [~ivan@unaffiliated/ivan/x-000001] has joined ##hplusroadmap 20:43 -!- ivan` [~ivan@unaffiliated/ivan/x-000001] has quit [Ping timeout: 260 seconds] 20:44 -!- nmz787 [~Nathan@107.53.239.200] has joined ##hplusroadmap 20:45 -!- ivan` [~ivan@unaffiliated/ivan/x-000001] has joined ##hplusroadmap 20:47 <@kanzure> nmz787: how was maradydd? 20:49 < nmz787> she didn't end up coming to hackmanhattan, said she was still jetlagged 20:49 < nmz787> and not feeling well 20:54 -!- jmil [~jmil@hive76/member/jmil] has quit [Ping timeout: 272 seconds] 21:03 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 21:06 <@kanzure> this seems sorta absurd https://community.rapid7.com/community/metasploit/blog/2012/08/21/metasploit-portable-ios 21:23 -!- mensch [~mensch@c-75-69-228-244.hsd1.ma.comcast.net] has quit [Quit: leaving] 21:31 -!- sylph_mako [~mako@84.35.252.27.dyn.cust.vf.net.nz] has joined ##hplusroadmap 21:32 -!- sylph_mako [~mako@84.35.252.27.dyn.cust.vf.net.nz] has quit [Read error: Connection reset by peer] 21:43 -!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap 21:43 -!- loanshark [~loanshark@ip72-218-133-8.hr.hr.cox.net] has quit [Quit: loanshark] 21:47 -!- nmz787 [~Nathan@107.53.239.200] has quit [Ping timeout: 260 seconds] 21:47 -!- nmz787 [~Nathan@107.53.239.200] has joined ##hplusroadmap 21:50 -!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Ping timeout: 260 seconds] 22:13 -!- tellurium [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has quit [Quit: I come here to insult and troll and ban people and have fun doing it. i can't be distracted from my playtime with these philosophical discussions] 22:20 -!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 22:22 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 22:29 -!- OldCoder is now known as YoungCoder 22:29 -!- YoungCoder is now known as LittleCoder 22:30 -!- LittleCoder is now known as GoldCoder 22:30 -!- GoldCoder is now known as SleepyCoder 22:31 -!- SleepyCoder is now known as ColdCoder 22:31 -!- ColdCoder is now known as OldCoder 22:33 < nmz787> this looks like a spicy meatball: "Gene Synthesis: Methods and Protocols (Methods in Molecular Biology, 852) 2012 Jean Peccoud" http://libgen.info/view.php?id=823673 22:36 < yashgaroth> "Leading a Successful iGEM Team" 22:36 <@kanzure> if that's not written by slovenian nationalists i would be suspicious of it 22:39 <@kanzure> pastebin for math: http://mathb.in/ 22:39 <@kanzure> maybe there should be a genetic pastebin called genebin 22:39 <@kanzure> it could even have shitty annotations and shitty plasmid visualization 22:40 < brownies> i thought there was already a latexbin? 22:40 < brownies> although admittedly this looks way, way better 22:40 <@kanzure> what's latexbin like? 22:41 < brownies> feels like 1996 22:41 <@kanzure> "pastebinit" used to be a great little tool until pastebin.com changed their api 22:41 <@kanzure> and now pastebinit requires -b http://paste.ubuntu.com/ or some other pastebin tacked on.. kinda ruins the point if you have to make a custom alias 22:42 < brownies> kanzure: couldn't you just alias it once in your bash profile and leave it at that... 22:42 < brownies> oh i see. that's too much work for you. alright. 22:43 <@kanzure> well, that's what i've done, yes 22:47 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 22:48 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 22:48 -!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap 22:51 -!- nmz787 [~Nathan@107.53.239.200] has quit [Ping timeout: 260 seconds] 23:09 <@kanzure> http://blog.mylookout.com/_media/Geinimi_Trojan_Teardown.pdf 23:20 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 23:21 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 23:50 -!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Quit: Leaving.] 23:51 -!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap 23:53 -!- tellurium [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has joined ##hplusroadmap --- Log closed Wed Aug 22 00:00:03 2012