--- Log opened Sun Jan 27 00:00:42 2013 00:18 -!- Charlie_ [~quassel@64.31.59.70] has quit [Quit: No Ping reply in 180 seconds.] 00:19 -!- Charlie [~quassel@64.31.59.70] has joined ##hplusroadmap 00:19 -!- Charlie is now known as Guest16281 00:22 -!- Mariu [Jimmy98@89.41.57.33] has quit [Ping timeout: 248 seconds] 00:37 -!- JayDugger [~duggerjw@pool-173-74-81-239.dllstx.fios.verizon.net] has joined ##hplusroadmap 00:38 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap 00:43 -!- Mariu [Jimmy98@89.41.57.33] has joined ##hplusroadmap 00:48 < JayDugger> I checked the logs without 00:48 < JayDugger> seeing 00:48 < JayDugger> this... 00:48 < JayDugger> http://www.cornyn.senate.gov/public/?a=Files.Serve&File_id=74c0afb3-1bc2-49f5-9150-0a8f004ef438 00:49 < JayDugger> Senator Cornyn (R-TX) sends Attorney General Holder a nasty letter re:Aaron Schwartz. 00:50 < JayDugger> Let Cornyn's office know what you think. http://www.cornyn.senate.gov/public/?a=Files.Serve&File_id=74c0afb3-1bc2-49f5-9150-0a8f004ef438 00:53 < kanzure> someone is claiming that FOIA can be used to get pdfs 00:53 < kanzure> i'm not sure if this is true, it sounds suspicious 00:54 < JayDugger> PDFs of what, kanzure. I've been arguing with my wife and my head is not on straight. 00:54 < JayDugger> papers? 00:54 < kanzure> papers. 00:54 < kanzure> JayDugger: never go to sleep with your wife angry, blah blah blah insert bogus marriage advice here 00:55 < brownies> that is an interesting twist 00:55 < JayDugger> Yeah...we got married. She has a house. I have housework--all of the house work. We also sleep on opposite schedules, so no big deal. 00:56 < JayDugger> All's well. I'll ask for housecleaning service next Christmas. :) 00:56 < kanzure> JayDugger: did you know that you can just pay people to clean your house? 00:56 < brownies> you're a stay-at-home dad? 00:56 < brownies> man, color me jealous. that sounds awesome. 00:56 < JayDugger> Gosh, no. I work third shift. 00:57 < brownies> oh =( 00:58 < JayDugger> As for FOIA getting papers, try muckrock.co, 00:58 < kanzure> brownies: so i think the FOIA claim is probably wrong. while the research is funded by tax dollars, the research institutions supposedly signed over the copyrights. 00:58 < JayDugger> .com, even. 00:58 < JayDugger> They'll file FOIA for you, IIRC. 00:59 < JayDugger> Here's there page for Silk Road. https://www.muckrock.com/foi/united-states-of-america-10/bitcoin-and-silk-road-1385/ 00:59 < kanzure> one FOIA per pdf that you want is horribly inefficient, since most requests are not processed immediately. 01:00 < JayDugger> Very, so even if it works, it'll suck. 01:00 < JayDugger> Here's muckrock for another Silk Road investigation from last year. https://www.muckrock.com/foi/united-states-of-america-10/bitcoin-and-silk-road-1385/ 01:01 < JayDugger> Oh, and I meant hiring housecleaners for Christmas. I'll have the house cleaned by then. 01:15 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 240 seconds] 01:22 -!- Mariu [Jimmy98@89.41.57.33] has quit [Ping timeout: 248 seconds] 01:35 -!- sylph_mako [~mako@103-9-42-1.flip.co.nz] has quit [Ping timeout: 245 seconds] 02:31 -!- u-metacognition [~metacogni@99-7-58-96.lightspeed.davlca.sbcglobal.net] has left ##hplusroadmap [] 02:34 -!- indigenous [~indigenou@85.210.234.50] has joined ##hplusroadmap 02:34 -!- indigenous [~indigenou@85.210.234.50] has quit [Changing host] 02:34 -!- indigenous [~indigenou@pdpc/supporter/student/indigenous] has joined ##hplusroadmap 02:36 < kanzure> hmm so apparently i sent an fbi foia request in 2009 and then again in 2010 with no formal response besides "we've received your request" 02:50 -!- phm [~anon@host-78-144-164-94.as13285.net] has joined ##hplusroadmap 03:29 < kanzure> "aaronsw scraped 900k books from google books that are in the public domain, but they haven't been edited yet http://archive.org/details/googlebooks " 03:30 < kanzure> ugh formatting http://archive.org/stream/zeitschriftfuer135unkngoog/zeitschriftfuer135unkngoog_djvu.txt 03:30 < kanzure> or erm.. i mean. ocr artifacts. 03:36 -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap 04:02 -!- eudoxia [~eudoxia@r186-53-134-203.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 04:12 < kanzure> hmm 04:20 -!- Urchin[Emacs] [~user@unaffiliated/urchin] has joined ##hplusroadmap 04:27 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has quit [Remote host closed the connection] 04:34 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 04:38 -!- barriers [~barriers@unaffiliated/barriers] has joined ##hplusroadmap 04:43 < kanzure> juri_: also, actual review of skdb specifications or contributions on that front would be pretty handy. none of the existing package managers really solve the same problems. 04:46 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 05:02 < kanzure> chris_99: do you have an athens login? 05:02 < chris_99> i don't think so 05:03 < kanzure> aren't you in the uk? 05:03 < chris_99> yeah 05:05 -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap 05:05 < kanzure> HEx1: what about you? athens? 05:05 -!- indigenous [~indigenou@pdpc/supporter/student/indigenous] has quit [Read error: Operation timed out] 05:07 < HEx1> hi kanzure. I've heard of it. but no, I don't currently have access to such a thing, if that's what you're asking 05:08 -!- indigenous [~indigenou@85.210.229.30] has joined ##hplusroadmap 05:08 -!- indigenous [~indigenou@85.210.229.30] has quit [Changing host] 05:08 -!- indigenous [~indigenou@pdpc/supporter/student/indigenous] has joined ##hplusroadmap 05:08 < kanzure> HEx1: okay, thank you. 05:09 -!- yorick [~yorick@ip51cd0513.speed.planet.nl] has joined ##hplusroadmap 05:09 -!- yorick [~yorick@ip51cd0513.speed.planet.nl] has quit [Changing host] 05:09 -!- yorick [~yorick@oftn/member/yorick] has joined ##hplusroadmap 05:12 -!- indigenous [~indigenou@pdpc/supporter/student/indigenous] has quit [Read error: Operation timed out] 05:15 < HEx1> is this related to journal scraping? 05:23 -!- Jaakko96 [~Jaakko@94-194-89-130.zone8.bethere.co.uk] has quit [Quit: Nettalk6 - www.ntalk.de] 05:26 < kanzure> HEx1: eh, not entirely.. i just want to see the athens control panel in action. and to examine cookies. 05:31 -!- eudoxia [~eudoxia@r186-53-134-203.dialup.adsl.anteldata.net.uy] has quit [Read error: Connection reset by peer] 05:33 -!- JayDugger [~duggerjw@pool-173-74-81-239.dllstx.fios.verizon.net] has quit [Quit: Leaving.] 05:34 -!- barriers [~barriers@unaffiliated/barriers] has quit [Read error: Connection reset by peer] 05:35 -!- barriers_ [~barriers@121-73-87-49.cable.telstraclear.net] has joined ##hplusroadmap 05:38 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 05:39 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8bfa7b2a Bryan Bishop: a few more biohacking articles 05:54 < gnusha_> https://secure.diyhpl.us/cgit/paperbot/commit/?id=e6842c14 Bryan Bishop: README: initial content 05:54 < gnusha_> https://secure.diyhpl.us/cgit/paperbot/commit/?id=32d476fa Bryan Bishop: README: add a link to phenny 05:54 < gnusha_> paperbot: reload papers 05:55 < kanzure> ah right that wont work because gnusha_ is not gnusha 05:56 < gnusha_> /nick gnusha 05:56 < kanzure> what. 06:00 -!- barriers [~barriers@unaffiliated/barriers] has joined ##hplusroadmap 06:01 -!- barriers_ [~barriers@121-73-87-49.cable.telstraclear.net] has quit [Read error: Connection reset by peer] 06:03 -!- barriers [~barriers@unaffiliated/barriers] has quit [Read error: Connection reset by peer] 06:03 -!- barriers [~barriers@unaffiliated/barriers] has joined ##hplusroadmap 06:04 < kanzure> remote: 19c9076..35682de master -> origin/master 06:04 < kanzure> remote: warning: inexact rename detection was skipped due to too many files. 06:04 < kanzure> remote: warning: you may want to set your merge.renamelimit variable to at least 1383 and retry the command. 06:05 < gnusha_> https://secure.diyhpl.us/cgit/paperbot/commit/?id=dfb9b34c Bryan Bishop: explicitly list some python dependencies 06:05 < gnusha_> https://secure.diyhpl.us/cgit/paperbot/commit/?id=88aff1a0 Bryan Bishop: README: describe another paperbot compulsion 06:05 < gnusha_> https://secure.diyhpl.us/cgit/paperbot/commit/?id=1fe6d64d Bryan Bishop: README: also also 06:05 < gnusha_> paperbot: reload papers 06:35 -!- SDr [SDr@unaffiliated/sdr] has quit [Read error: Connection reset by peer] 06:35 -!- SDr [SDr@sdrinf-2-pt.tunnel.tserv5.lon1.ipv6.he.net] has joined ##hplusroadmap 06:39 -!- barriers [~barriers@unaffiliated/barriers] has quit [Read error: Connection reset by peer] 06:40 -!- barriers_ [~barriers@121-73-87-49.cable.telstraclear.net] has joined ##hplusroadmap 06:55 -!- panax [panax@131.247.116.2] has quit [Ping timeout: 244 seconds] 06:56 -!- panax [~panax@68.200.160.182] has joined ##hplusroadmap 07:14 -!- phm [~anon@host-78-144-164-94.as13285.net] has quit [Quit: Leaving] 07:14 -!- panax [~panax@68.200.160.182] has quit [Ping timeout: 252 seconds] 07:15 -!- panax [panax@131.247.116.2] has joined ##hplusroadmap 07:32 -!- EnLilaSko- [~Nattzor@host-85-30-145-65.sydskane.nu] has joined ##hplusroadmap 07:32 -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has quit [Ping timeout: 245 seconds] 07:33 -!- EnLilaSko- is now known as EnLilaSko 07:33 -!- EnLilaSko [~Nattzor@host-85-30-145-65.sydskane.nu] has quit [Changing host] 07:33 -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap 09:01 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 09:06 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 09:13 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 09:15 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 10:10 -!- _sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 252 seconds] 10:10 -!- eudoxia [~eudoxia@r186-53-137-81.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 10:21 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 10:23 -!- yorick [~yorick@oftn/member/yorick] has quit [Remote host closed the connection] 10:25 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 10:27 -!- SDr [SDr@sdrinf-2-pt.tunnel.tserv5.lon1.ipv6.he.net] has quit [Changing host] 10:27 -!- SDr [SDr@unaffiliated/sdr] has joined ##hplusroadmap 10:29 -!- He||eshin is now known as Helleshin 10:37 -!- eudoxia [~eudoxia@r186-53-137-81.dialup.adsl.anteldata.net.uy] has quit [Remote host closed the connection] 11:05 -!- _Sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap 11:08 < archels> http://25.media.tumblr.com/c7ad6ab2b58160a897fe1e23f264a122/tumblr_mfttyuUHbf1qjmk64o1_500.jpg 11:15 -!- favetelinguis [zwoop@202-154-140-242.ubs-dynamic.connections.net.nz] has joined ##hplusroadmap 11:34 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 11:36 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 11:59 -!- yorick 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joined ##hplusroadmap 13:11 -!- strangewarp [~C@c-67-173-247-251.hsd1.co.comcast.net] has quit [Disconnected by services] 13:11 -!- ksjdhfksjdhfksd is now known as strangewarp 13:41 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has quit [Remote host closed the connection] 13:48 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 13:50 < kanzure> paperbot: http://pubs.acs.org/doi/abs/10.1021/cr030203g 13:50 < paperbot> error: HTTP 500 http://diyhpl.us/~bryan/papers2/paperbot/Nonaqueous%20Liquid%20Electrolytes%20for%20Lithium-Based%20Rechargeable%20Batteries.pdf 13:51 < kanzure> paperbot: http://www.ncbi.nlm.nih.gov/pubmed/10442032 13:51 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/dffaabb6175762ffafcd3064b3853b2b.txt 13:52 -!- indigenous [~indigenou@85.210.229.101] has joined ##hplusroadmap 13:52 -!- indigenous [~indigenou@85.210.229.101] has quit [Changing host] 13:52 -!- indigenous [~indigenou@pdpc/supporter/student/indigenous] has joined ##hplusroadmap 14:10 < kanzure> "We've started up an EastBay DIYBio group (we've met twice so far in Downtown Oakland at www.sudoroom.org , a great, new and welcoming melded hackerspace) and we'd love to invite anyone interested in meeting with us the last Friday of each month in the East bay to come and join us!" 14:10 < kanzure> https://groups.google.com/group/east-bay-diybio 14:11 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has quit [Remote host closed the connection] 14:13 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f4990753 Bryan Bishop: add east-bay-diybio group 14:19 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 14:28 -!- Radivis [~quassel@217.248.149.72] has quit [Remote host closed the connection] 14:37 < favetelinguis> hi all, anyone from sweden on here? 14:39 -!- EnLilaSko- [~Nattzor@host-85-30-145-65.sydskane.nu] has quit [Quit: - nbs-irc 2.39 - www.nbs-irc.net -] 14:40 < kanzure> favetelinguis: splicer is in sweden. 14:46 -!- xx [~xx@dsl-66-36-145-222.mtl.aei.ca] has quit [Read error: Connection reset by peer] 14:46 -!- arizonaaa [~xx@dsl-66-36-145-222.mtl.aei.ca] has joined ##hplusroadmap 14:57 -!- arizonaaa is now known as xx 15:07 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has quit [Remote host closed the connection] 15:09 < favetelinguis> cant see him online now, will look out for him, thanks 15:21 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 15:25 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 15:26 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 15:35 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 15:38 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 16:00 -!- u-metacognition [~metacogni@99-7-58-96.lightspeed.davlca.sbcglobal.net] has joined ##hplusroadmap 16:01 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 16:12 -!- favetelinguis [zwoop@202-154-140-242.ubs-dynamic.connections.net.nz] has quit [Ping timeout: 252 seconds] 16:26 -!- u-metacognition [~metacogni@99-7-58-96.lightspeed.davlca.sbcglobal.net] has left ##hplusroadmap [] 16:37 -!- yorick [~yorick@oftn/member/yorick] has quit [Read error: Connection reset by peer] 16:40 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has quit [Remote host closed the connection] 16:51 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 16:55 -!- Urchin[Emacs] [~user@unaffiliated/urchin] has quit [Remote host closed the connection] 17:44 -!- qu-bit_ [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 17:45 -!- favetelinguis [zwoop@202-154-140-242.ubs-dynamic.connections.net.nz] has joined ##hplusroadmap 17:46 < kanzure> favetelinguis: welcome back 17:47 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has quit [Ping timeout: 276 seconds] 17:55 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has quit [Remote host closed the connection] 17:56 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has joined ##hplusroadmap 18:20 -!- archbox_ [~archbox@unaffiliated/archbox] has quit [Quit: bye] 18:20 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 18:32 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has joined ##hplusroadmap 18:49 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Quit: the neuronal action potential is an electrical manipulation of reversible abrupt phase changes in the lipid bilayer] 18:50 < kanzure> web inspector commits rss feed http://peter.sh/data/web-inspector-rss.php 18:52 < kanzure> http://paulirish.com/2011/web-browser-frontend-and-standards-feeds-to-follow/ 19:10 < kanzure> yashgaroth: is sheldrake more or less of a quack than hameroff? 19:10 < kanzure> i misplaced my quack decoder ringer. sheldrake is the "morphogenetic field" guy. :/ 19:10 < yashgaroth> I have luckily not heard of either of them 19:11 < yashgaroth> however from a brief googling they are both detrimental to humanity 19:11 < kanzure> k 19:11 < kanzure> the wikipedia article on "morphogenetic field" looks like a sane thing now, it looks like they shoved all of sheldrake's stuff into a subsection. 19:13 < yashgaroth> yeah that'll happen 19:18 -!- qu-bit_ [~shroedngr@gateway/tor-sasl/barriers] has quit [Ping timeout: 276 seconds] 19:19 < kanzure> lab protocol videos http://www.benchfly.com/ 19:19 < kanzure> "opening a locked ultracentrifuge" http://www.benchfly.com/video/50/opening-a-locked-ultracentrifuge/ 19:20 < kanzure> http://www.benchfly.com/video/49/opening-a-locked-eppendorf-centrifuge/ 19:20 < kanzure> http://www.benchfly.com/video/47/opening-a-locked-j2-21/ 19:20 < kanzure> http://www.benchfly.com/video/46/opening-a-locked-sorvall-rc5c/ 19:20 < kanzure> http://www.benchfly.com/video/45/opening-a-locked-rt6000/ 19:21 < kanzure> hm it seems to be lacking the yucky biology protocols 19:21 < kanzure> http://www.benchfly.com/video/30/how-to-mail-dna/ 19:22 < yashgaroth> "How to Chunk Worms" FINALLY 19:22 < yashgaroth> looks pretty good though 19:22 < kanzure> jove has more stuff 19:23 < yashgaroth> yeah but it's all super-specific stuff 19:23 < kanzure> http://www.jove.com/video/50192/optogenetic-stimulation-of-escape-behavior-in-drosophila-melanogaster 19:23 < kanzure> http://www.jove.com/video/50104/forebrain-electrophysiological-recording-in-larval-zebrafish 19:24 < yashgaroth> like that 19:24 < kanzure> http://www.jove.com/video/50079/isolation-and-culture-of-mouse-cortical-astrocytes 19:24 < kanzure> heh http://www.jove.com/video/4243/manual-drainage-of-the-zebrafish-embryonic-brain-ventricles 19:25 < yashgaroth> "You do not have access to any JoVE content through your current IP address." whaaaa 19:25 < kanzure> paperbot: http://www.jove.com/video/4285/a-visual-description-dissection-cerebral-surface-vasculature 19:25 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/A%20Visual%20Description%20of%20the%20Dissection%20of%20the%20Cerebral%20Surface%20Vasculature%20and%20Associated%20Meninges%20and%20the%20Choroid%20Plexus%20from%20Rat%20Brain.pdf 19:26 < yashgaroth> since when is jove paywalled?! 19:26 < kanzure> always was 19:27 < kanzure> .. right? 19:27 < yashgaroth> I seem to recall viewing full videos, lemme find a non-neuro one and see if it happens again 19:27 < yashgaroth> wow nope it's everything 19:28 < yashgaroth> I seem to recall it was free at some point, way back 19:28 < kanzure> someone should do a youtube poop of these protocol videos 19:29 < yashgaroth> maybe it was back when I had a UW proxy or something, oh well 19:29 < kanzure> and accurately show how enraging they are 19:30 < yashgaroth> I thought youtube poop videos were strictly limited to 90's cartoons based off of video games 19:33 < kanzure> paperbot: http://ecsource.jove.com/CDNSource/4154_Levin_Pathogenesis_050412_F_Web.mov 19:33 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/7006f6fbb24865d33c2fb09592c7b39d.txt 19:34 < kanzure> jove stops playing after 30 seconds through pdx.edu 19:34 < kanzure> but it seems that they did this in flash, and the actual video file is still the full content 19:37 < yashgaroth> I was gonna say, should be possible to extract it 19:37 < kanzure> that text file there is a mov file. quicktime is playing it just fine. 19:39 < yashgaroth> yep, works for me 19:39 < kanzure> these people do not look happy at all 19:40 < yashgaroth> why are they wearing lab coats in the office? that is not osha compliant 19:40 -!- technicus [~technicus@ip-64-134-160-6.public.wayport.net] has joined ##hplusroadmap 19:41 < kanzure> paperbot: http://www.jove.com.proxy.lib.pdx.edu/video/4163/visualization-genetic-manipulation-dendrites-spines-mouse-cerebral 19:41 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Visualization%20and%20Genetic%20Manipulation%20of%20Dendrites%20and%20Spines%20in%20the%20Mouse%20Cerebral%20Cortex%20and%20Hippocampus%20using%20In%20utero%20Electroporation.pdf 19:42 < kanzure> paperbot: http://ecsource.jove.com/CDNSource/4163_Guillemot_071812_F_Web.mov 19:42 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/b16c71f79078e122239477f887d013d7.txt 19:44 < kanzure> "Transfer the animal to the second pad. Begin the surgery when the animal loses the twitching reflex." yessss. 19:46 -!- technicus [~technicus@ip-64-134-160-6.public.wayport.net] has quit [] 19:46 < kanzure> "Avoid applying current across the placenta, as this will cause embryo death. Try to electroporate all of the embryos in under 30 minutes." 19:50 < kanzure> paperbot: http://www.jove.com.proxy.lib.pdx.edu/video/4031/production-lentiviral-vectors-for-transducing-cells-from-central 19:50 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/404f6b938dc7b584cf87a4b3112ff094.txt 19:51 < kanzure> paperbot: http://ecsource.jove.com/CDNSource/4031_Snider_Astrocytes_051612_F_Web.mov 19:51 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/29abccb2d19b5417dd5889054bbe1084.txt 19:54 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 19:57 < kanzure> whaat she didn't switch out tips 19:59 < kanzure> i wonder where they found voice talent that can pronounce this shit. 20:03 < kanzure> i need a way to loop all of these videos 20:08 < yashgaroth> academics don't switch out tips without a good reason 20:08 < kanzure> i guess i spent more time working with dna 20:09 < kanzure> you must be paranoid when working with dna, rna and other small molecules because even minor contamination throws off everything. 20:11 < kanzure> "Remove all the fetuses and place them in a sterile dish prepared earlier. Cut the embryos from the amniotic sac using a pair of small scizzors. Place them in a second dish. Wash the embryos at room temperature by gently tilting the dish for 5 to 10 seconds. Transfer the rinsed embryos to another dish. Under a stereomicroscope, extract each rat's brain with a pair of curved forceps. Place about 5 brains in a 60mm dish." 20:11 < yashgaroth> when a single molecule will fuck you over, yeah 20:11 < kanzure> how do you have "about" 5 brains 20:11 < kanzure> http://ecsource.jove.com/CDNSource/3965_Peruzzi_051612_F_Web.mov 20:12 < yashgaroth> add 1 tablespoon of embryonic rat brains, to taste 20:15 < kanzure> "This can be performed in about 2 hours if you perform it properly." ha, ha 20:16 < kanzure> http://ecsource.jove.com/CDNSource/3621_Morrow_Morphogenesis_051412_F_Web.mov 20:19 < kanzure> "using a custom-made hamilton syringe, ..." wait, that's not fair. 20:23 -!- favetelinguis [zwoop@202-154-140-242.ubs-dynamic.connections.net.nz] has quit [Ping timeout: 255 seconds] 20:38 < kanzure> "a very narrow needle track minimizes brain damage" i'd say. 20:48 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Quit: Lost terminal] 20:51 -!- lichen [~lichen@24.21.206.64] has joined ##hplusroadmap 20:53 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has quit [Remote host closed the connection] 21:02 -!- favetelinguis [zwoop@202-154-140-242.ubs-dynamic.connections.net.nz] has joined ##hplusroadmap 21:08 -!- qu-bit [~shroedngr@gateway/tor-sasl/barriers] has joined ##hplusroadmap 21:25 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has quit [Quit: Leaving] 21:32 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 21:48 < kanzure> "MBA programs are more brazen about this. It's a regular thing for an MBA professor to reschedule exams to accommodate student travel and social events. Top MBAs are the only educational program (to my knowledge) where professors are virtually required to accommodate students' social calendars." 21:48 < kanzure> "The tuition bubble is about the social connections. Do not be deluded on this. Education is a secondary concern in all that, especially when we're talking about private pre-schools where the quality is ancillary: it's about the "feeder" effect. How did degenerate connection-seeking get conflated with education?" 21:48 < kanzure> http://news.ycombinator.com/item?id=5126714 21:58 -!- phm [~anon@host-78-144-164-94.as13285.net] has joined ##hplusroadmap 22:04 -!- u-metacognition [~metacogni@99-7-58-96.lightspeed.davlca.sbcglobal.net] has joined ##hplusroadmap 22:10 < brownies> have you ever noticed how the only people who are anti-college are the ones who didn't graduate? 22:10 < brownies> where are all the Harvard grads who think college is a bad idea? 22:11 < yashgaroth> ehh I graduated and I'm anti 22:12 < brownies> nonsense. where's your cognitive dissonance! 22:12 < joehot> if you graduated of course youre going to like it 22:12 < yashgaroth> it's a bit different for bio since you can't just drop out and do freelance biology in silicon valley 22:13 < brownies> you could've gotten a job, though 22:13 < brownies> biology has other... obstacles... to freelancing. even for graduates =P 22:13 < yashgaroth> in biology? washing glassware maybe 22:13 < yashgaroth> or filling vials in a cleanroom 22:13 < brownies> ah, so you admit the college education was necessary 22:14 < brownies> you just don't want other people to have it? =P 22:14 < yashgaroth> that's what, like, society says, man 22:14 < kanzure> hehe people use excel for bioinformatics? http://nsaunders.wordpress.com/2012/10/22/gene-name-errors-and-excel-lessons-not-learned/ 22:14 < yashgaroth> I'm against the idea that college education is required to learn a subject 22:14 < kanzure> brownies: jojack graduated from harvard and he thinks it's a fucking awful idea 22:14 < brownies> i'd like to hear from him, then 22:14 < kanzure> he doesn't seem to be online at the moment 22:14 < brownies> he thinks going to Harvard is a bad idea? 22:15 < brownies> or he thinks going to Shitty State U is a bad idea? 22:15 < kanzure> he had a lot of friends kill themselves while he was stationed at harvard 22:16 < kanzure> he has an extremely negative view of college. 22:16 < yashgaroth> jesus what 22:16 < brownies> not sure how many qualifies for "a lot" but it is actually relatively common at all top universities 22:16 < kanzure> yes 22:16 < yashgaroth> man I only had one and I thought that was a lot 22:16 < yashgaroth> wait, two 22:16 < kanzure> brownies: i was only mentioning the suicides because you would probably claim that his experience is different from others. but he has other reasons based on reasoning. 22:17 < brownies> yeah i would be genuinely curious to hear them. 22:17 < brownies> in the meantime... yashgaroth: why do you think it's a bad idea? 22:17 < yashgaroth> because all I did in college was get high and read textbooks and papers 22:17 < yashgaroth> 2/3 I could do on my own, now 3/3 with paperbot 22:18 < brownies> i know people who did that. 22:18 < brownies> it seems the simple solution is to not do that. 22:18 < brownies> *not do just that. 22:18 < yashgaroth> how do you mean 22:19 < brownies> well, now that i'm out of college, i look back on it as a rare time that i had to just lead a life dedicated to studying and learning ... a privilege which i'll probably never have again 22:19 < brownies> in theory, it would have been nice to have been told that at the start of the 4 years, rather than realizing it years later. 22:19 < brownies> in practice... i'm not sure the message would've sunk in 22:20 < brownies> but if it had! then people would not just sit around. 22:20 < yashgaroth> true 22:20 < kanzure> how does that require college? 22:20 < kanzure> what?? 22:22 < brownies> the professors, labs, and fellow students are the part that college provides 22:22 < brownies> otherwise, yeah, you can totally sit at home, eat pizza, and read textbooks all day. as long as you make rent, nothing's stopping you. 22:23 < kanzure> did you know that professors still exist even if you don't pay tuition? 22:23 < yashgaroth> so college provides connections? 22:23 < kanzure> did you know that you can still go to classes even without tuition? 22:23 < brownies> yes, but what does that prove? 22:23 < brownies> i am aware that all of these things exist 22:23 < kanzure> that nothing you have said supports your thesis 22:23 < brownies> college gives you an environment where you are surrounded by that, with a dedicated feedback loop to help you learn 22:23 < kanzure> you would know this if you went to college... oh wait. 22:24 < brownies> nothing rules out creating this for yourself from scratch 22:25 < brownies> you could farm all your food too, and make your own microchips in your oven, but here you are going to the grocery store and buying laptops off-the-shelf 22:25 * kanzure shrugs 22:25 < kanzure> homecmos seems to be working out okay 22:26 < yashgaroth> let's not slide down the slippery slope too quick here 22:26 < kanzure> yashgaroth: https://www.facebook.com/photo.php?fbid=657182149541&set=a.657181545751.2225972.9591&type=1&relevant_count=4 22:27 < brownies> kanzure: you will note that my whole thesis is just anti-anti-college ... which just requires college to have a > 0 utility. 22:27 < yashgaroth> omg that profile pic 22:28 < yashgaroth> college undeniably has utility, it's just a question of whether it's worth the cost 22:28 < yashgaroth> /time 22:30 < kanzure> yashgaroth: https://www.facebook.com/notes/joseph-p-jackson/my-friend-the-killer/471914364559 22:31 -!- Hu_Meanan [~quassel@199.48.197.18] has joined ##hplusroadmap 22:31 -!- Hu_Meanan [~quassel@199.48.197.18] has quit [Changing host] 22:31 -!- Hu_Meanan [~quassel@unaffiliated/humean] has joined ##hplusroadmap 22:31 < yashgaroth> "This content is currently unavailable" maybe I should friend him 22:31 < kanzure> jojack is hilarious "PS. When you have an extensive library of melodic death metal, as I do, there is always a song for every occasion." 22:32 < kanzure> damn none of his facebook notes have a writeup of his views on college 22:34 < kanzure> " I am personally an anomaly, having never used a journal or any kind of formal record for diet and training tracking. Getting ripped is pretty simple--start with 2 100 meter sprints a day, increase over 12-18 wks until you work up to 20-30 intervals in a 24 hr period. When you feel like vomitting, which is most of the time, you know you are doing it right." 22:34 < kanzure> fascinating methods! 22:34 < kanzure> "We pay for convicts to lift weights in the joint damn it, why can't I catch a break?" 22:36 < yashgaroth> 100 meter sprints are a vital part of my patented 'resisting arrest' fitness program 22:36 < yashgaroth> the sprints burn fat, while the tazing and beating stimulate muscle growth 22:37 < yashgaroth> recommended supplements include a good lawyer 22:37 -!- HARMANZ [~quassel@199.48.197.18] has joined ##hplusroadmap 23:23 -!- yashgaroth [~ffffff@cpe-66-27-118-94.san.res.rr.com] has quit [Quit: Leaving] 23:41 -!- lichen [~lichen@24.21.206.64] has quit [Read error: Connection reset by peer] 23:43 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap 23:54 < kanzure> yashgaroth: don't leave me bro 23:54 < kanzure> http://www.utsandiego.com/news/2013/jan/26/do-it-yourself-biotech-spread/ 23:56 < kanzure> "The website diybio.org lists 16 groups in the United States, including Sunnyvale, known as BioCurious, San Francisco (BioBridge) and Los Angeles (LA BioHackers)." 23:56 < kanzure> pfft my list is more thorough 23:58 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=5d8ee282 Bryan Bishop: add a diybio article about the san diego group 23:59 < kanzure> http://diyhpl.us/wiki/diybio/groups 23:59 < kanzure> by my count there are 45 --- Log closed Mon Jan 28 00:00:43 2013