--- Log opened Thu Feb 14 00:00:00 2013
00:10 < nmz787> kanzure: $1500 looks like he's using the same projector like you sent me http://sedgwick3d.com/
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00:39 < nmz787> "By using this system we were able to do custom photolithography in a much shorter time that we could have using
00:39 < nmz787> masks. The time between the formulation of an idea to a
00:39 < nmz787> completed project can be less than 20 min."
00:43 < nmz787> kanzure: this is cool http://www.thingiverse.com/thing:29735
00:44 < nmz787> i'm not sure what it means 'with reprap technologies'
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01:01 <@kanzure> nmz787: have you looked at lemoncurry?
01:02 <@kanzure> wtf happened to thingiverse's stylesheet.
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01:17 < nmz787>  paperbot http://144.206.159.178/FT/CONF/16419087/16419119.pdf
01:17 < nmz787> kanzure: that shows drop storage
01:17 <@kanzure> gotta have the colon
01:17 <@kanzure> paperbot: http://144.206.159.178/FT/CONF/16419087/16419119.pdf
01:17 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/37083933a3ea7dff998d78616918def6.pdf
01:17 < nmz787> kanzure: yeah i've looked at it, not in a week or so
01:18 < nmz787> when i was just scanning it for resin links
01:18 < nmz787> i found a UK company that has cheap resin, but shipping is crazy expensive
01:18 <@kanzure> anyway, here's what i was reading on droplet storage:
01:18 <@kanzure> http://diyhpl.us/~bryan/papers2/microfluidics/Simple,%20robust%20storage%20of%20drops%20and%20fluids%20in%20a%20microfluidic%20device.pdf
01:18 < nmz787> something about their post office having an embargo for sending hazardous materials to the u.s.
01:22 < nmz787> kanzure: do you have the accompanying autocad file?
01:22 < nmz787> "An AutoCAD le of the actual device
01:22 < nmz787> is included in the ESI (S7.dwg)."
01:30 <@kanzure> nope not on me. ESI sounds like "electronic supplementary information".
01:34 <@kanzure> i wonder if the zotero translators are able to pick up links to supplementary pdfs
01:35 <@kanzure> if not, that's a rather serious omission.
01:35 < nmz787> that would be nice
01:35 < nmz787> they're often not pdfs though
01:35 < nmz787> sometimes avi files, or in this case dwg
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04:56 < chris_99> has anyone used http://www.addgene.org/
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07:15 < strangewarp> http://www.nytimes.com/2013/02/15/sports/oscar-pistorius-shooting-south-africa.html?_r=2& - Oscar Pistorius, first person to quality for the Olympics while using prosthetics, and noted for his usefulness as a rhetorical device in transhumanist discussions, murders girlfriend
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07:42 < ThomasEgi> sounds like the story around reiserfs
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12:27 < delinquentme> effing langton labs website is down
12:27 < delinquentme> =[
12:27 < delinquentme> poopy blogspot
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12:59 <@kanzure> http://repo.steampowered.com/steam/pool/steam/s/steam/steam64_1.0.0.27_amd64.deb
12:59 < ThomasEgi> yay. 64bit linux steam
13:01 < nmz787> is that really hplus related?
13:01 < nmz787> not that i care
13:06 < chris_99> am i right in thinking something like this plasmid http://www.addgene.org/22308/ would create GFP when inserted into yeast
13:06 < nmz787> yes
13:06 < nmz787> when galactose is added
13:07 < chris_99> aha ok, cheers
13:08 <@kanzure> nmz787: no, it's not. lapse of judgement.
13:10 < nmz787> chris_99: http://en.wikipedia.org/wiki/Gal_operon
13:11 < chris_99> thanks
13:11 <@kanzure> "reminiscent of the case of Gordon & Breach v. American Institute of Physics and American Physical Society which started with the publication of articles on the cost of physics journals by H.H. Barschall"
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13:12 <@kanzure> http://www.insidehighered.com/news/2013/02/08/academic-press-sues-librarian-raising-issues-academic-freedom
13:12 < chris_99> also i've been looking at this http://labs.fhcrc.org/gottschling/Yeast%20Protocols/ytrans.html the bottom protocol, so is it more or less a case of charging a 25uF cap, then discharging via a 200 ohm resistor to the cuvette
13:13 <@kanzure> what is Edwin Mellen Press anyway?
13:13 < nmz787> chris_99: electroporation has a few general methods
13:14 < nmz787> the type of decay, a cap is exponential, is important and can be played with to give different results
13:14 < nmz787> length of decay, voltage
13:14 < nmz787> i was unsuccessful at transforming ecoli with a grill lighter piezo sparker
13:14 < chris_99> mm, which is varied with the resistor i guess, and the initial charging voltage
13:14 < chris_99> aha
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13:15 < nmz787> but that was just based on the sparker putting out some average value i got from diybio
13:15 < nmz787> and then changing the distance between electrodes
13:15 < nmz787> so i must have been way off in some paramaeter
13:17 < nmz787> it looks like that protocol wants SS-DNA, but I'm not sure if they mean ssDNA (single-stranded)
13:17 < chris_99> oh i didn't notice that
13:17 < nmz787> oh it was the first protocol
13:17 < nmz787> with LiAc
13:18 < chris_99> hmm, i've got a PSU that can do KV hopefully, i'd need to get the necessary caps / resistors though.
13:18 < nmz787> " Give one pulse: V= 1.5kV, 25 F, 200 Ohms. Time should be ~4-5""
13:18 < nmz787> 4-5 minutes???
13:18 < nmz787> i wonder if you could charge it with a piezo sparker
13:19 < nmz787> they're around 10kv i think
13:19 < nmz787> if i recall correctly
13:19 < archels> 1.5 kV over what electrode spacing?
13:19 < nmz787> i'm bad when it comes to capacitors and charge and discharge
13:19 < nmz787> 0.2 cm
13:19 < chris_99> 0.2cm cuvette
13:20 < nmz787> chris_99: that means you could use a 0.1cm cuvette with 750V
13:20 < nmz787> i'm not sure if you'd need to change the ohms though
13:20 < chris_99> do http://www.addgene.org/ ship to residential addresses?
13:20 < nmz787> prob not
13:21 < nmz787> from what i heard recently you need to be non-profit or academic
13:21 < nmz787> industrial users only have access to a subset of the dna
13:21 < archels> you guys just need to build some Cockcroft-Walton voltage boosters
13:21 < chris_99> mm, i got some caps to do that
13:21 < nmz787> the professional electroporator i took apart used SCRs if that's worth anything
13:22 < archels> chris_99: great, just add diodes =)
13:22 < chris_99> is there anywhere else that would send plasmids
13:24 <@kanzure> sciquest is asking for me to make a website with a picture of my product
13:24 <@kanzure> i don't have a product. quick, someone make up a product!
13:25 < nmz787> send openspectrometer
13:25 <@kanzure> ah good idea
13:26 <@kanzure> the deed has been done.
13:28 < archels> actually, I think laser printers have DC high voltage modules
13:30 < chris_99> mm, i think your right
13:35 < nmz787> kanzure: what's sciquest?
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13:35 < nmz787> omg i have this jalokia pepper beef stick
13:35 < nmz787> its made me hiccup
13:41 < nmz787> ahh, it's a text file
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13:43 < nmz787> kanzure: they have asection on select agents
13:44 < nmz787> SE L E C T AGE NT S :
13:44 < nmz787> Definition: Some products are live cells of extremely hazardous pathogens such as Ebola virus, Marburg virus etc.
13:44 < nmz787> The CDC has set stringent regulations about all aspects o
13:44 < nmz787> products.
13:44 < nmz787> Usage: This Product Flag should be turned on for any product that is considered to be a select agent as per CDC
13:44 < nmz787> definition.
13:44 < nmz787> Example: Monkeypox virus, Brucella melitensis, Bovine spongiform e
13:44 < nmz787> Note: Additional details about CDC regulations about select agents can be
13:44 < nmz787> http://www.cdc.gov/od/sap/docs/salist.pdf
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13:54 <@kanzure> sciquest is just some lab equipment leasing.. thing.
13:56 < nmz787> kanzure: do you have any papers on light controlled microfluidic valves?
13:56 < nmz787> I saw something yesterday that showed that
13:57 < nmz787> i was thinking it might be nice if the energy densities are practical with a DLP, though I doubt they are, I'm not sure at all
13:57 < nmz787> I'm figuring out energy density stuff now for resins
13:57 <@kanzure> not really
13:57 <@kanzure> http://diyhpl.us/~bryan/papers2/microfluidics/Single-sided%20continuous%20optoelectrowetting%20(SCOEW)%20for%20droplet%20manipulation%20with%20light%20patterns%20-%20LCD%20-%202010.pdf
13:58 <@kanzure> http://diyhpl.us/~bryan/papers2/microfluidics/Light-driven%20droplet%20manipulation%20technologies%20for%20lab-on-a-chip%20applications.pdf
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14:09 < nmz787> kanzure: heard of 3dsystems.com?
14:10 <@kanzure> yes.
14:10 <@kanzure> dassault-owned, so it's a heavy tie-in to solidworks.
14:13 < nmz787> are there any $500-$750 range 3d printers available off-the-shelf?
14:14 <@kanzure> reprap.
14:14 <@kanzure> http://www.ebay.com/sch/i.html?_from=R40&_sacat=0&_nkw=prusa+reprap&_sop=3
14:15 < nmz787> are there nicer looking chinese ripoffs?
14:16 <@kanzure> why would you care about nicer looking :(
14:16 < nmz787> to get a better feel for how to redeisgn one
14:17 <@kanzure> http://wiki.reprap.org/
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14:17 <@kanzure> also, there's 100s of people in #reprap that you should talk with
14:17 < nmz787> this looks much cleaner http://cubify.com/cube/
14:17 < nmz787> than this http://www.ebay.com/itm/3D-Printer-reprap-prusa-mendel-complete-package-prototyping-UP-Plus-NEW-/111011737070?pt=LH_DefaultDomain_0&hash=item19d8d0adee
14:17 <@kanzure> i guarantee that you will get better information from #reprap
14:17 < nmz787> ahh
14:18 <@kanzure> seems they are up to 463 people today.
14:18 < nmz787> that wiki just says it works!
14:19 <@kanzure> wow wtf
14:19 <@kanzure> ah they moved it
14:19 <@kanzure> http://reprap.org/wiki/Main_Page
14:22 <@kanzure> nmz787: i also recommend /join #lemoncurry
14:27 <@kanzure> http://www.adafruit.com/blog/2013/02/12/limor-fried-ladyada-joining-president-obama-in-a-fireside-hangout-this-thursday-at-450pm-est-on-google/
14:27 <@kanzure> limor fried and obama? sounds good to me.
14:32 < streety> is anyone watching that? I've only just got in
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14:32 <@kanzure> i think it's at 43min 30sec
14:32 <@kanzure> this isn't what i was expecting. it's just a few questions she sent in.
14:33 <@kanzure> she's just asking about software patents.
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14:35 < streety> fair enough, I've just joined the live stream at 1hr5min
14:44 < streety> the end was quite good, ". . . are we done?"
14:45 <@kanzure> http://www.reddit.com/r/Scholar/comments/18gwnk/removing_watermarks_from_academic_papers/
14:46 <@kanzure> "Nota bene: removing a watermark will shatter your chances of calling paper-sharing "fair use" if challenged in court"
14:46 <@kanzure> does he have any evidence?
14:47 < ParahSailin> i dont think thats too wild of an assertion
14:47 <@kanzure> not what i'm asking... case law.
14:47 < ParahSailin> court precedent is publishers always win and prosecutors are overzealous
14:48 <@kanzure> i haven't seen anything about an academic watermark ever being used in court.
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15:01 < nmz787> kanzure: adduser or useradd?
15:01 < nmz787> kanzure: which makes all the dirs and stuff?
15:03 <@kanzure> adduser
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17:06 <@kanzure> xkcd's discussion about steroids is horribly under-informed http://forums.xkcd.com/viewtopic.php?f=7&t=100281
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17:11 < bkero> Surprise?
17:12 <@kanzure> yes, it was a pro-steroid comic but the responses are all negative.
17:20 < streety> Is that so surprising? If you disagree with the comic would you not be more likely to comment?
17:20 <@kanzure> yes, usually xkcd responses include links to wikipedia and shit, but even the pro-steroids users aren't posting those refutations.
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17:23 < streety> A higher % of first-timers perhaps?
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17:50 <@kanzure> paperbot: http://europepmc.org/abstract/MED/8969015/reload=0;jsessionid=PcANClGdQ0ZNoS0uvDpy.6
17:50 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/78289f60d27e97973ee4cf6b2ab0017b.txt
17:51 <@kanzure> paperbot: http://www.sciencedirect.com/science/article/pii/S0306453002000252
17:51 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Neuroendocrine%20and%20behavioral%20effects%20of%20high-dose%20anabolic%20steroid%20administration%20in%20male%20normal%20volunteers.pdf
17:57 <@kanzure> "Psychoneuroendocrinology" is an almost ridiculous journal title
17:58 < yashgaroth> "scandinavian journal of experimental psychoneuroendocrinology"
18:00 < strangewarp> the xkcd forums are full of people who love feeling smart without doing the legwork
18:00 <@kanzure> "soviet journal of letters in experimental psychoneuroendocrinology, volume ш"
18:00 <@kanzure> hm i wonder if there's a "soviet journal" still around somewhere
18:00 <@kanzure> aha... Soviet Journal of Physical Oceanography.
18:00 <@kanzure> Soviet Journal of Experimental
18:00 <@kanzure> and Theoretical Physics
18:01 <@kanzure> aww they dropped the "soviet" from the title http://www.springer.com/physics/particle+and+nuclear+physics/journal/11447
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18:17 <@kanzure> paperbot: http://www.springer.com/realtimeTopDownload/download/fe6c875d-00fe-460f-97c5-3ad5eb4e91f8/10.1007/s12024-010-9198-1.pdf
18:17 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/c3763bacaf5e38a22fbfdcf01dd8e804.txt
18:18 <@kanzure> CVE-2012-1089 "Directory traversal vulnerability in Apache Wicket 1.4.x before 1.4.20 and 1.5.x before 1.5.5 allows remote attackers to read arbitrary web-application files via a relative pathname in a URL for a Wicket resource that corresponds to a null package"
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19:08 < SphericalMouse> Sup?
19:11 < docl> howdy
19:12 < SphericalMouse> any excitement?
19:12 < JayDugger> Happy Valentine's Day!
19:13 < docl> I'm working on a wiki for cryo stuff
19:13 < archbox_> JayDugger: you too
19:13 <@kanzure> docl: would you consider just using diyhpluswiki.git?
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19:14 <@kanzure> ParahSail1n: hey did you happen to read the backlog with SphericalMouse yesterday?
19:14 < ParahSail1n> i missed it
19:14 < SphericalMouse> he's a terrible typist
19:15 < docl> kanzure: any particular reason I should?
19:16 <@kanzure> docl: because putting transhumanist information in the same place increases the likelihood that contributors will maintain the information.
19:17 < ParahSail1n> what was the backlog about, im on a different machine from usual
19:18 < docl> my theory is that multiple different sites/communities is good for diversity or some such. also my ego is more flattered at the thought of starting my own thing.
19:19 <@kanzure> ParahSail1n: SphericalMouse is interested in lab automation, and also has some experience with microfluidics.
19:20 <@kanzure> docl: what about just forking the repo? :)
19:20 < docl> hmm. maybe. I'm using foswiki at the moment.
19:20 < docl> what's diyhpluswiki.git in?
19:20 <@kanzure> have you ever used git?
19:20 <@kanzure> well, it's just a collection of files that can be rendered any way you please. i use ikiwiki.
19:20 <@kanzure> http://diyhpl.us/wiki is the front-end
19:21 <@kanzure> https://github.com/kanzure/diyhpluswiki is the git repo
19:21 < ParahSail1n> SphericalMouse, thats cool
19:21 < docl> I've played around a bit with github
19:21 < SphericalMouse> indeed, what is your interest in the area?
19:21 <@kanzure> docl: the downside is that the default ikiwiki stylesheets don't look like mediawiki's monobook.css layout. i guess that might be one reason you might not use this.
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19:22 < ParahSail1n> i think it would be cool to have a cell sorter
19:23 < SphericalMouse> what kind of cells?
19:23 < ParahSail1n> mammal i suppose
19:23 < ParahSail1n> facs is super expensive
19:24 < SphericalMouse> what do you want to sort based upon?
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19:24 < ParahSail1n> if i had one, im sure i could think of a few things to do
19:25 < SphericalMouse> well, there are different ways to sort things
19:25 < docl> for now, the wiki is at http://bettercryonics.com/BetterCryonics
19:27 < SphericalMouse> bead/surface binding, dielectrophoresis, electrophoresis, size based, density based
19:27 < SphericalMouse> blah blah
19:27 < ParahSail1n> most of the sorting id want to do would probably be based on fluor markers
19:28 < SphericalMouse> urff
19:29 < ParahSail1n> it would be nice to just have a flow cytometer to take measurements of population even
19:29 < SphericalMouse> I suppose you could flow things at low density down a y channel and have some force push things from one side to another
19:29 < SphericalMouse> oh, thats pretty simple, as things go
19:30 < SphericalMouse> inverted flourescent microscope with camera
19:30 < ParahSail1n> i did some experiments on facscanto, but it would be pretty cool to be able to afford one
19:31 < SphericalMouse> what do you have?
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19:33 < ParahSail1n> well i was a grad student for a while doing wet lab, now i'm kinda out of that area
19:34 < SphericalMouse> what equipment or facilities do you have access to?
19:34 < ParahSail1n> a pirate version of the agilent bioanalyzer would be cool too, for qc of ngs library prep
19:34 < ParahSail1n> nothing at the moment
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19:35 <@kanzure> docl: do you mind if i import content into diyhpluswiki.git?
19:35 < docl> I don't mind
19:36 < SphericalMouse> how much are you willing to spend?
19:37 < ParahSail1n> it depends on the upside?
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19:38 < SphericalMouse> say you could take pictures of cells, extract the fluorescence level, and look at size and shape? would that be what you want?
19:38 < nmz787> howdy
19:39 < ParahSail1n> well, i could probably find a good deal on that sort of stuff on slapsale
19:40 < SphericalMouse> on what?
19:41 < ParahSail1n> fluorescence microscopy
19:41 < nmz787> hey a site that isn't ebay!
19:41 < nmz787> any ideas on how to hook up a fridge peltier to act as a still chiller?
19:42 < SphericalMouse> everclear is pretty cheap
19:44 < SphericalMouse> well, what would your overall budget be?
19:45 < nmz787> this looks pretty nice http://www.slapsale.com/item_desc.aspx?itemid=63601
19:46 < nmz787> hm, maybe not
19:46 < nmz787> sorry
19:46 < SphericalMouse> unfortunately, that is not a brand of fluorescent microscope i am familiar with
19:47 < nmz787> hey there are a few good looking deals, ParahSail1n is this all local pickup?
19:47 < ParahSail1n> nmz787, yes
19:48 < nmz787> this scope is pretty nice if the price stays low http://www.slapsale.com/item_desc.aspx?itemid=63705
19:49 < SphericalMouse> usually you want an inverted microscope
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19:50 < SphericalMouse> especially for micofluidics
19:50 < nmz787> why?
19:51 < SphericalMouse> various accesibility reasons. often there will be plumbing, which is easier to deal with if ti comes out the top, but then it bangs into the objectives of an upright scope
19:51 < nmz787> huh, why don't people just make manifolds that have sideports?
19:52 < nmz787> like a CPU socket
19:52 < SphericalMouse> ~lolzz~
19:52 < nmz787> where an arm comes and closes over the top
19:52 < nmz787> compresses down
19:53 < SphericalMouse> oftne pdms microfluidics will have a thin and thick side, and a scope wont be able to image through the thick side, andyou want the thin side down
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19:54 < SphericalMouse> every fluid connection i have ever seen comes in through the top or bottom, never the side
19:54 < nmz787> weird
19:54 < SphericalMouse> consequence of manufacturing
19:55 < SphericalMouse> and geometry
19:55 < JayDugger> Would you have ullage problems without gravity draining the channel?
19:55 < ParahSail1n> yeah ive only seen vias at top and bottom
19:55 < ParahSail1n> gravity can't make enough pressure to make flow through microchannel
19:56 < JayDugger> Fair enough.
19:56 < SphericalMouse> you can have gravity driven flow issues, typially it is low compared to other forces, but you can see it if you're not careful
19:56 < SphericalMouse> just blance your inlet ant outlet heights
19:56 < SphericalMouse> goddamn wine
19:58 < SphericalMouse> I would use about 5-10 psi do drive fluid through microchannels, internet says 1 psi is a delta of 27 inches of water
20:05 < SphericalMouse> i have seen people use sliding vials on rails as a pressure source though
20:07 < nmz787> i think i saw something like that not too long ago
20:08 < SphericalMouse> i alsways used a gas line with a tunable regulator, but the rail will work as well, probably more accurate, but getting 5 psi would require a 10 footish rail
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21:51 <@kanzure> Juul: do you have the storage space configured yet?
21:51 <@kanzure> for libgen?
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22:07 <@kanzure> it looks like mendeley might be interested in donating rule 34 relevant artwork for launching springerkink.com
22:08 <@kanzure> yashgaroth: it will be glorious
22:09 < yashgaroth> not even sure what to say to that
22:10 < Juul> kanzure, storage space? i have a 4 tb harddrive but only about 15% downloaded
22:10 <@kanzure> well i'm just tired of not having access to springerlink.com
22:10 < Juul> it's been taking longer than it should due to some suspicious network issues
22:10 < Juul> (possibly trolls but could be stupidity)
22:11 < Juul> basically someone came in and hard reset two routers
22:13 < yashgaroth> do you share the routers with people?
22:13 < yashgaroth> if you overloaded the router and someone else using it got pissed off and reset it
22:15 < yashgaroth> then again I have no idea what your setup is
22:27 < Juul> yashgaroth, i rate limited everything. but yeah it was in a shared space. we've been getting trolled though, so it may be trolls at work
22:28 < Juul> they also fucked the network by doing that since it created a rogue dhcp server
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--- Log closed Fri Feb 15 00:00:01 2013