--- Log opened Thu May 02 00:00:02 2013
--- Day changed Thu May 02 2013
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00:04 < jeffz> hi, anyone with a US university institutional login have access to this? http://babel.hathitrust.org/cgi/pt?id=mdp.39015039846566
00:04 < jeffz> trying to get the full pdf
00:07 < kanzure> bleh you don't need that.
00:08 < jeffz> I do if i want to read it without the pages constantly reloading when I scroll through it.
00:09 < jeffz> just thought someone might be able to do me a favor
00:09 < kanzure> i mean, perceptrons aren't exactly that great
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00:35 < kanzure> google glass winking api https://news.ycombinator.com/item?id=5642924
00:35 < kanzure> http://glass-apps.org/winky-google-glass-app
00:37 < kanzure> https://plus.google.com/116031914637788986927/posts/Hp9n6oXpFfH
00:37 < kanzure> https://github.com/kaze0/winky
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01:20 < chido> ~.
01:20 < chido> ~>
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02:57 < archels> "Google Glass is the future – and the future has awful battery life"
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06:50 < Urchin> hi people
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11:34 < delinquentme> jmil, I bought a magazine.
11:34 < delinquentme> you were in it. gj
11:37 < kanzure> https://github.com/kanzure/pdfparanoia/pull/25 "I've written a script tests/diff/comparediff which automates downloading PDFs from two different sources, and verifying that pdfparanoia makes the files byte-for-byte identical."
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11:44 < DonnchaC> kanzure: Nice work, I was gonna implement something similar in the next few weeks
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11:46 < jmil> delinquentme: wha?
11:48 < delinquentme> jmil, it was a science mag
11:48 < delinquentme> i guess whats crazy though is how long it takes that information to get into magazines
11:48 < jmil> delinquentme: wow cool. know which one?
11:48 < delinquentme> are you still working on that?
11:48 < jmil> still working on sugar printing for organ printing? YES. it is a 30 yr project and I'm all in on it
11:49 < delinquentme> It seems that decellularization seems a quicker path to that end :D
11:49 < jmil> starting my own research lab at Rice University as assistant professor of bioengineering in a couple months
11:49 < delinquentme> i guess why not perfusion decellularization though
11:49 < jmil> delinquentme: decellularization is interesting, but it has no control and no reproducibility (every organ is different). they don't have a reproducible way to re-seed cells into the tissue.
11:49 < delinquentme> other than " I have money for 3d printing sugar structures "
11:49 < delinquentme> what?
11:50 < delinquentme> are you sure?
11:50 < delinquentme> from what I've read its simply an issue of negative pressure in the right areas...
11:50 < jmil> i am watching the space carefully. it is certainly very interesting. but i've talked to the experts in person and they admit total lack of control or ways to assay the tissue, which will HAVE to be done before it gets into a human patient
11:51 < kanzure> DonnchaC: i didn't write it
11:51 < jmil> the risk of blood clots is insanely high. in fact, that's why they always fail
11:51 < jmil> so far
11:51 < kanzure> jmil: let me know when you are going to be stocking your lab. i'd be happy to suggest toys and goodies.
11:51 < jmil> kanzure: serious? that would be aweosme
11:52 < delinquentme> assay the tissue for what?
11:52 < jmil> delinquentme: yes well that hasn't even been defined yet
11:52 < jmil> i am developing a research program to actually define what we need to assay
11:52 < DonnchaC> kanzure: woops, just assumed you did from the git comments I read on my phone earlier
11:52 < delinquentme> I'm missing something here.  You need to qualify what things we need to look at pre-implantation?
11:52 < jmil> delinquentme: for example, of the cells injected, where do they go in the tissue hierarchy? how many stay alive?
11:52 < kanzure> DonnchaC: no, that was a contribution from someone else
11:53 < jmil> delinquentme: you can't do imaging in a block of tissue that big. so if you have a huge life-threatening blood clot in the center of the structure you will never know it until it kills the patient
11:53 < DonnchaC> Yeah I see that now, useful none the less
11:53 < delinquentme> I mean you know they've done nothing more than apply negative pressure, circulate a fluid of cells and then apply given physical stimulus ... and subsequently gotten lungs running in a rat right?
11:53 < kanzure> jmil, what did you think of that recent hydrogel/imaging paper?
11:54 < jmil> delinquentme: yes, but that was only for a few hours before it clotted out
11:54 < jmil> kanzure: which paper?
11:54 < kanzure> the CLARITY paper
11:55 < jmil> delinquentme: i'm very interested in decellularization, but it lacks a certain level of control which i desire for quantitative cell biology studies
11:55 < kanzure> this one, http://diyhpl.us/~bryan/papers2/paperbot/Structural%20and%20molecular%20interrogation%20of%20intact%20biological%20systems.pdf
11:55 < kanzure> oh oops
11:55 < kanzure> i mean http://diyhpl.us/~bryan/papers2/paperbot/7b0c273e70ca41c9d5e28f4efef62ada.pdf
11:55 < kanzure> it does things like:
11:55 < kanzure> http://www.nature.com/nature/journal/vaop/ncurrent/fig_tab/nature12107_SV14.html "Hippocampus region showing networks of YFP-expressing neurons (green), distribution of PV-positive neurons (red) and astrocytes (blue)."
11:56 < jmil> kanzure: ya that is cool tech. i have a collaborator who knows how to do Scale. She mentioned CLARITY too
11:56 < jmil> in Houston
11:56 < jmil> at Baylor
11:56 < kanzure> "Second, whereas passive diffusion of detergent micelles would take many months to completely extract lipids from the adult mouse brain, we developed an active-transport organ-electrophoresis approach, which we term electrophoretic tissue clearing (ETC), that capitalizes on the highly charged nature of ionic micelles."
11:56 < kanzure> so anyway, organ electrophoresis
11:57 < jmil> kanzure: yes it appears to work well, and i'm very familiar with acrylate monomers
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13:09 < delinquentme> http://www.youtube.com/watch?v=DiyWXQyAJ44
13:09 < delinquentme> cat vs. crocodile
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13:12 < jrayhawk> alligator
13:17 < jrayhawk> alligators are relatively timid
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13:56 < superkuh> paperbot: http://www.sciencemag.org/content/339/6118/452.long
13:56 < paperbot> error: HTTP 300 http://diyhpl.us/~bryan/papers2/paperbot/Actin%2C%20Spectrin%2C%20and%20Associated%20Proteins%20Form%20a%20Periodic%20Cytoskeletal%20Structure%20in%20Axons.pdf
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14:27 < ParahSai1in> michael j fox foundation is trying to insert human lrrk2 bac into a mouse genome
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14:41 < kanzure> have i mentioned how much i hate javascript
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14:43 < ThomasEgi> kanzure, now you did.
14:50 < ParahSai1in> lol i've been playing with javascript to make an internal browser app
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14:50 < ParahSai1in> i might have to rewrite in clojurescript or something
14:51 < ParahSai1in> shits all just a mess
14:52 < ThomasEgi> i only used javascript to build very simple media-player applications for a company. for that kind of stuff you can get away with 10 lines of javascript. compared with a lot of code in other languages.
14:52 < ThomasEgi> guess for everything else javascript is pretty much a pita.
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15:45 < abetusk> Did you guys see this: http://www.youtube.com/watch?feature=player_embedded&v=oRWeD2VFh7w
15:45 < kanzure> .title
15:45 < yoleaux> DNA testing chip delivers results in one hour, paves way for personalized drug treatments #DigInfo - YouTube
15:45 < kanzure> yes, the news articles claimed it was sequencing, but it's pcr
15:46 < abetusk> How close are we to doing this on a DIY level?
15:46 < kanzure> pcr?
15:46 < kanzure> uh, we've been doing pcr for a long time now..
15:46 < abetusk> No, miniaturized
15:47 < kanzure> you can pcr a droplet of water with a laser if you wanted, is that small enough for you
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16:14 < superkuh> paperbot: http://stm.sciencemag.org/content/4/140/140ra88.full.pdf
16:14 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/82ee57c6013289b2b2e749a29c27fce1.txt
16:15 < superkuh> paperbot: http://stm.sciencemag.org/content/4/140/140ra88.full
16:15 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/c0091b11fec76a81da1ee0eb65cc9b2c.txt
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21:21 < nmz787_> ParahSai1in: TCD1304AP is the CCD I'm working with, it's the same one as the nanodrop my school had
21:21 < nmz787_> ParahSai1in: do you layout PCBs at all? ever use kicad?
21:22 < kanzure> CERN is trying to fundraise a few euros to hire someone to work on kicad full-time
21:23 < nmz787_> yeah but not to help with projects, that's to make the software better
21:23 < nmz787_> but yes that's good news
21:23 < nmz787_> http://blog.al.com/wire/2013/03/ancient_underwater_forest_off.html
21:23 < nmz787_> pretty cool
21:23 < kanzure> i think juri_ uses gEDA so maybe bug her about layout things
21:24 < kanzure> juri_: are you also a layout wizard? i think you mentioned something the other day to this effect.
21:29 < nmz787_> geez i just assume everyone on here is a dude
21:29 < nmz787_> guess that's the faceless old internet
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22:22 < ParahSail1n> nmz787_, no, i dont have much electronics experience
22:24 < ParahSail1n> nmz787_, what do you have so far?
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--- Log closed Fri May 03 00:00:15 2013