--- Log opened Mon May 20 00:00:32 2013 00:02 -!- yashgaroth [~ffffff@cpe-66-27-118-94.san.res.rr.com] has quit [Quit: Leaving] 00:08 -!- Juul [~Juul@c-67-170-193-114.hsd1.ca.comcast.net] has joined ##hplusroadmap 00:11 -!- Juul_ [~Juul@static.3.202.4.46.clients.your-server.de] has joined ##hplusroadmap 00:15 -!- Juul [~Juul@c-67-170-193-114.hsd1.ca.comcast.net] has quit [Ping timeout: 264 seconds] 00:54 < kanzure> i think i will do some midnight soul searching on c2 http://c2.com/cgi/wiki?ObjectiveEvidenceAgainstGotos 00:56 < superkuh> http://order.foc.us/ 00:56 -!- Juul_ [~Juul@static.3.202.4.46.clients.your-server.de] has quit [Ping timeout: 256 seconds] 00:56 < kanzure> "Make your synapses fire faster." 00:57 < kanzure> um.. 00:58 < kanzure> "The headset passes a small electric current (<2.05mA) through the prefrontal cortex of the wearer." well that's vague. 01:17 -!- Juul [~Juul@static.3.202.4.46.clients.your-server.de] has joined ##hplusroadmap 01:36 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Ping timeout: 256 seconds] 01:47 -!- biolulz [~biolulz@host86-166-147-214.range86-166.btcentralplus.com] has joined ##hplusroadmap 02:21 -!- Juul [~Juul@static.3.202.4.46.clients.your-server.de] has quit [Ping timeout: 256 seconds] 02:59 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 03:40 -!- Viper168_ [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 03:42 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 252 seconds] 03:46 -!- makoLime [~mako@103-9-42-128.flip.co.nz] has quit [Ping timeout: 264 seconds] 03:48 -!- FooQuuxman [~test@c-98-215-254-55.hsd1.il.comcast.net] has joined ##hplusroadmap 03:55 -!- Juul [~Juul@208.87.217.74] has joined ##hplusroadmap 04:23 -!- kmo [~kmo@unaffiliated/kmo] has quit [Read error: Operation timed out] 04:24 -!- archels` [~neuralnet@sascha.esrac.ele.tue.nl] has quit [Read error: Operation timed out] 04:24 -!- archels [~neuralnet@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 04:26 -!- kmo [~kmo@unaffiliated/kmo] has joined ##hplusroadmap 04:53 -!- Juul [~Juul@208.87.217.74] has quit [Remote host closed the connection] 05:04 -!- cogitokat [~kat@ip70-171-26-151.ga.at.cox.net] has joined ##hplusroadmap 05:06 -!- ielo [~ielo@134.219.227.25] has joined ##hplusroadmap 05:13 -!- ielo [~ielo@134.219.227.25] has quit [Ping timeout: 248 seconds] 05:14 -!- yorick [~yorick@oftn/member/yorick] has joined ##hplusroadmap 05:18 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has joined ##hplusroadmap 05:31 -!- nmz787 [~nmz787@131.252.130.248] has quit [Ping timeout: 264 seconds] 05:31 -!- nmz787 [~nmz787@131.252.130.248] has joined ##hplusroadmap 05:31 -!- Viper168_ [~Viper@unaffiliated/viper168] has quit [Ping timeout: 260 seconds] 05:40 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 05:41 -!- Urchin[emacs] [~user@unaffiliated/urchin] has joined ##hplusroadmap 05:43 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has quit [Read error: Connection reset by peer] 05:43 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has joined ##hplusroadmap 06:03 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Read error: Connection timed out] 06:04 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 06:40 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Read error: Connection timed out] 06:42 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 07:29 -!- ParahSailin [~Rob@unaffiliated/parahsailin] has joined ##hplusroadmap 07:39 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Read error: Connection timed out] 07:43 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 08:13 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Read error: Connection timed out] 08:21 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 08:46 < kanzure> there is a company called 'bioglow' that seems to be doing glowing things :( http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/aphis_response_bioglow_032113.pdf 08:47 < brownies> is this different from the kickstarter guys? 08:52 < kanzure> yes 08:52 < kanzure> pffft "postdocpocalypse" 09:21 < brownies> good. the more companies we have on this, the faster i can get a glowing cat. 09:22 < kanzure> soon you too will hate glowing things 09:22 < kanzure> and your rebels friend are walking into a trap, as is your pathetic fleet. 09:22 -!- eudoxia [~eudoxia@r186-52-34-56.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 09:22 < kanzure> *rebel friends 09:22 < eudoxia> oh shit mike's blog is down http://chronopause.com/ 09:23 < brownies> probably will cost a fortune in cat food. 09:31 -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection] 09:34 < eudoxia> .meow 09:34 < yoleaux> http://moar.edgecats.net/cats/tumblr_mm5bjfDbG61qdlh1io1_400.gif 09:53 -!- klafka [~klafka@204-16-157-18-static.ipnetworksinc.net] has joined ##hplusroadmap 10:03 -!- klafka [~klafka@204-16-157-18-static.ipnetworksinc.net] has quit [Read error: Connection reset by peer] 10:03 -!- klafka_ [~klafka@204-16-157-18-static.ipnetworksinc.net] has joined ##hplusroadmap 10:09 -!- augur [~augur@129-2-129-33.wireless.umd.edu] has joined ##hplusroadmap 10:21 -!- eudoxia [~eudoxia@r186-52-34-56.dialup.adsl.anteldata.net.uy] has left ##hplusroadmap ["leaving"] 10:25 < chris_99> paperbot: http://ieeexplore.ieee.org/xpl/login.jsp?tp=&arnumber=6103636&url=http%3A%2F%2Fieeexplore.ieee.org%2Fxpls%2Fabs_all.jsp%3Farnumber%3D6103636 10:25 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/99ecfee2e69401f616cb1373b7755806.txt 10:27 < kanzure> it would help if you try the abs_all link itself instead of the login.jsp link 10:29 < chris_99> aha 10:29 < chris_99> paperbot: http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=6103636 10:29 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/b04c418d67db99df5aecd89b0299ebdb.txt 10:41 -!- ielo [~ielo@cpc9-addl4-2-0-cust229.6-3.cable.virginmedia.com] has joined ##hplusroadmap 10:43 -!- OldCoder_ [~OldCoder_@adsl-64-169-36-85.dsl.snfc21.pacbell.net] has joined ##hplusroadmap 10:44 -!- ielo [~ielo@cpc9-addl4-2-0-cust229.6-3.cable.virginmedia.com] has quit [Client Quit] 10:45 -!- ryankarason [~rak@cpe-76-189-227-224.neo.res.rr.com] has joined ##hplusroadmap 11:03 -!- biolulz [~biolulz@host86-166-147-214.range86-166.btcentralplus.com] has quit [Quit: biolulz] 11:22 -!- yorick [~yorick@oftn/member/yorick] has quit [Remote host closed the connection] 11:44 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 256 seconds] 11:48 -!- biolulz [~biolulz@cpc5-walt14-2-0-cust382.13-2.cable.virginmedia.com] has joined ##hplusroadmap 12:15 -!- randallagordon [~randall@67-5-189-117.ptld.qwest.net] has quit [Ping timeout: 240 seconds] 12:17 -!- randallagordon [~randall@75-175-60-198.ptld.qwest.net] has joined ##hplusroadmap 12:23 -!- randalla1ordon [~randall@67-5-163-121.ptld.qwest.net] has joined ##hplusroadmap 12:25 -!- oblique [~oblique@unaffiliated/oblique] has joined ##hplusroadmap 12:27 -!- randallagordon [~randall@75-175-60-198.ptld.qwest.net] has quit [Ping timeout: 264 seconds] 12:28 < oblique> hello guys, what is your opinion about http://foc.us ? isn't too expensive? 12:30 < chris_99> i bet you could make something similar a lot cheaper 12:31 < kanzure> and their marketing sucks, and wouldn't you prefer something open source, and a few other issues 12:31 -!- randallagordon [~randall@67-5-171-221.ptld.qwest.net] has joined ##hplusroadmap 12:33 < chris_99> can you combine EEG + tDCS to see the effect of the stimulation 12:33 < oblique> kanzure: i prefer opensource, but i don't know anything about electrical engineering, i only know programming 12:33 < kanzure> maybe you should fix that? i promise it is worthwhile. 12:34 < kanzure> chris_99: i haven't seen an effective demonstration of that. if you only care about whether or not the eeg sensors light up, i guess that's okay. but you could also just use an oscilloscope.. 12:34 -!- randalla1ordon [~randall@67-5-163-121.ptld.qwest.net] has quit [Ping timeout: 264 seconds] 12:36 < oblique> kanzure: are there any good opensource tdcs? openstim is good and safe? 12:38 < kanzure> /home/gnusha/public_html/logs/2012-07-09.log:16:01 < kanzure> openstim was basically just ed boyden throwing some parts together and then never publishing anything, i think 12:39 < kanzure> /home/gnusha/public_html/logs/2008-12-04.log:06:15 < kanzure> openstim is dead 12:39 < oblique> so, any alternatives? 12:40 < biolulz> loving that outdoor chick photoshoot though. Appeal to that "gamer demographic" ?!! 12:40 < kanzure> there was another tdcs marketing stunt from last year that never materialized, it was also proprietary 12:40 < kanzure> if you want things to happen, the best way to proceed is to do it yourself 12:40 < kanzure> http://diyhpl.us/~bryan/papers2/neuro/tdcs/ 12:41 < oblique> thx! 12:42 < oblique> rdb was thinking to build something good, but he 12:42 < oblique> is busy with real life 12:42 < kanzure> so what? 12:44 < oblique> well, someone with good knowledge have to do it 12:57 -!- augur [~augur@129-2-129-33.wireless.umd.edu] has quit [Remote host closed the connection] 12:59 -!- yorick [~yorick@oftn/member/yorick] has joined ##hplusroadmap 13:19 -!- makoLime [~mako@103-9-42-128.flip.co.nz] has joined ##hplusroadmap 13:35 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Quit: replacing bad RAM] 14:03 -!- biolulz [~biolulz@cpc5-walt14-2-0-cust382.13-2.cable.virginmedia.com] has quit [Read error: Connection reset by peer] 14:05 -!- biolulz [~biolulz@cpc5-walt14-2-0-cust382.13-2.cable.virginmedia.com] has joined ##hplusroadmap 14:10 -!- biolulz [~biolulz@cpc5-walt14-2-0-cust382.13-2.cable.virginmedia.com] has quit [Quit: biolulz] 14:14 -!- Thomas42 [~Thomas42@static.105.236.9.5.clients.your-server.de] has quit [Read error: Connection reset by peer] 14:19 -!- makoLime [~mako@103-9-42-128.flip.co.nz] has quit [Ping timeout: 248 seconds] 14:19 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 14:19 -!- Thomas42 [~Thomas42@static.105.236.9.5.clients.your-server.de] has joined ##hplusroadmap 14:25 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 14:33 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 14:44 < kanzure> "Flickr gets new UI, new Android app, 1TB free space" oh hm, maybe that's where i can store all my things 14:45 < fenn> my hobby is taking pictures of seemingly random noise 14:46 < kanzure> stop criticising my artistic vision 14:46 < kanzure> it is a very very very specific vision 14:47 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has quit [Quit: - nbs-irc 2.39 - www.nbs-irc.net -] 14:50 < kanzure> unfortunately i don't think gzip streams look random 15:24 -!- Mokstar [~Moktart@unaffiliated/mokstar] has joined ##hplusroadmap 15:24 < Mokstar> Whoo! 15:24 < Mokstar> ThomasEgi, So, would you just have a pair of pins, and change the pinmode from output to input really fast? 15:25 -!- FooQuuxman [~test@c-98-215-254-55.hsd1.il.comcast.net] has quit [Ping timeout: 256 seconds] 15:27 < Mokstar> I wish I had smart :( 15:28 < ThomasEgi> what was it you'r trying to do again? 15:32 < ThomasEgi> anyway. i'm lacking sleep. the best advice i can give you is not to listen to my advice right now^ 15:33 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 15:34 < Mokstar> Hah :p 15:34 < Mokstar> tRNS 15:34 < Mokstar> but you're gone now 15:41 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has joined ##hplusroadmap 15:57 < ParahSailin> 1TB is a lot of papers 15:57 < ParahSailin> 1 TB per captcha solution that is 16:02 < kanzure> deathbycaptcha is something like $0.0001/captcha 16:09 -!- FooQuuxman [~test@c-98-215-254-55.hsd1.il.comcast.net] has joined ##hplusroadmap 16:35 -!- underscor [~quassel@192.210.211.75] has joined ##hplusroadmap 16:35 -!- underscor is now known as Guest23706 17:00 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has quit [Ping timeout: 260 seconds] 17:11 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has joined ##hplusroadmap 17:24 -!- Guest23706 is now known as underscor 17:26 -!- klafka_ [~klafka@204-16-157-18-static.ipnetworksinc.net] has quit [Remote host closed the connection] 18:15 -!- FooQuuxman [~test@c-98-215-254-55.hsd1.il.comcast.net] has quit [Read error: Connection reset by peer] 18:15 -!- FooQuuxm1n [~test@c-98-215-254-55.hsd1.il.comcast.net] has joined ##hplusroadmap 18:19 -!- FooQuuxm1n is now known as FooQuuxman 18:26 -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 18:31 < heath> kanzure: http://diyhpl.us/~bryan/nucleic/fbi-diybio-dna-v1.pdf is the second result for "diy dna synthesizer" in an ingognito mode browser over here 18:33 < kanzure> yep 18:34 -!- yashgaroth [~ffffff@cpe-66-27-118-94.san.res.rr.com] has joined ##hplusroadmap 18:46 -!- Urchin[emacs] [~user@unaffiliated/urchin] has quit [Ping timeout: 248 seconds] 18:48 -!- yorick [~yorick@oftn/member/yorick] has quit [Remote host closed the connection] 19:09 -!- FooQuuxman [~test@c-98-215-254-55.hsd1.il.comcast.net] has quit [Ping timeout: 248 seconds] 19:19 -!- yoleaux [~yoleaux@213.138.101.38] has quit [Quit: dpk made me do it!] 19:20 -!- yoleaux [~yoleaux@obquire.infologie.co] has joined ##hplusroadmap 19:32 -!- FooQuuxman [~test@c-98-215-254-55.hsd1.il.comcast.net] has joined ##hplusroadmap 20:26 -!- Grognor [a9e8d417@gateway/web/freenode/ip.169.232.212.23] has joined ##hplusroadmap 20:29 < heath> nmz787: so what's the status on your project? 20:30 < heath> i just finished watching https://www.youtube.com/watch?v=b6zpEyDvdRA 20:31 -!- Mooookstar [~Moktart@c-98-246-107-5.hsd1.or.comcast.net] has joined ##hplusroadmap 20:35 -!- Mokstar [~Moktart@unaffiliated/mokstar] has quit [Ping timeout: 276 seconds] 20:43 -!- Mokbort [~Moktart@c-98-246-107-5.hsd1.or.comcast.net] has joined ##hplusroadmap 20:46 -!- Mooookstar [~Moktart@c-98-246-107-5.hsd1.or.comcast.net] has quit [Ping timeout: 276 seconds] 20:47 -!- Viper168 [~Viper@node84.19.251.72.1dial.com] has joined ##hplusroadmap 20:47 -!- Viper168 [~Viper@node84.19.251.72.1dial.com] has quit [Changing host] 20:47 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 20:57 -!- FooQuuxman [~test@c-98-215-254-55.hsd1.il.comcast.net] has quit [Ping timeout: 252 seconds] 21:00 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has quit [Ping timeout: 252 seconds] 21:03 -!- Mokbort [~Moktart@c-98-246-107-5.hsd1.or.comcast.net] has quit [Ping timeout: 276 seconds] 21:04 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has joined ##hplusroadmap 21:10 -!- Burninate [~Burn@pool-108-18-159-219.washdc.fios.verizon.net] has quit [Read error: Connection reset by peer] 21:11 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 246 seconds] 21:11 -!- Burninate [~Burn@pool-108-18-159-219.washdc.fios.verizon.net] has joined ##hplusroadmap 21:18 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 21:35 -!- Adifex [~Adifex@97-122-125-109.hlrn.qwest.net] has joined ##hplusroadmap 21:49 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has quit [Ping timeout: 260 seconds] 21:49 -!- kmo [~kmo@unaffiliated/kmo] has quit [Quit: Leaving] 21:50 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has joined ##hplusroadmap 21:57 -!- makoLime [~mako@wireless-nat-7.auckland.ac.nz] has quit [Ping timeout: 248 seconds] 22:16 -!- kirka [~Kirka@109.205.253.139] has joined ##hplusroadmap 22:17 -!- OldCoder_ [~OldCoder_@adsl-64-169-36-85.dsl.snfc21.pacbell.net] has quit [Remote host closed the connection] 22:20 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Quit: the neuronal action potential is an electrical manipulation of reversible abrupt phase changes in the lipid bilayer] 22:23 < kirka> Hi guys 22:23 < kirka> Hi, kanuzre 22:23 < kirka> kanzure 22:23 < Adifex> sup 22:24 < kanzure> kirka: welcome back. 22:25 < kirka> kanzure Thanks for http://diyhpl.us/~bryan/papers2/bio/ , these are interesting papers 22:25 < kanzure> you might be interested in http://diyhpl.us/~bryan/papers2/longevity/ 22:25 < kanzure> and http://diyhpl.us/~bryan/papers2/microfluidics/ 22:26 < kirka> I have started reading some textbooks on molecular biology some time ago 22:26 < Adifex> by the way, I'm looking for a bio-related project or two on github or sourceforge that could use some help with python. Does anyone have any suggestions? 22:29 < kanzure> biopython 22:30 < kirka> kanzure Don't you know a good book on E.coli? Something that describes current understanding of it's genome functionality. I have found Escherichia coli: virulence mechanisms of a versatile pathogen, but it's not exactly what I want 22:31 < kanzure> the current understanding of its genome is on ncbi's website 22:32 < kirka> It'd be cool to have "genome atlas" of E.Coli where it's proteins and metabolic pathway would be described in detail 22:32 < kirka> Ah, genome annotation 22:32 < Adifex> hm. that looks pretty cool, thanks 22:34 < kirka> kanzure Do you think that biocyc is a good idea? It's propietary though 22:34 < kanzure> i think the other databases are good 22:37 < kirka> I'm reading "Molecular biology of the cell", but it's very general 22:38 < klafka> good book 22:38 < klafka> i read that 22:39 < kirka> It'll take some time to read these 1200+ pages 22:39 < kanzure> you should also read the other books here: http://diyhpl.us/wiki/diybio/faq/books 22:40 < kirka> I have read the SB primer already, it's a good introduction 22:40 < kirka> good list 22:44 < Adifex> who runs the diyhpl.us site? 22:45 < kanzure> i do. 22:46 < Grognor> only death awaits the unprepared https://twitter.com/RachelHaywire/status/336669832734965760 22:47 -!- Grognor [a9e8d417@gateway/web/freenode/ip.169.232.212.23] has left ##hplusroadmap [] 22:47 < kanzure> uh.. please don't bring that in here. 22:54 < kanzure> can't help but point out that 169.232.212.23 is los angeles (where rachel lives) 22:56 < kanzure> wasn't this the homeless person? 22:58 < kirka> btw I have my own toy C.Elegans simulator now: http://i.imgur.com/BlOJP9L.jpg 700 lines of CL code, 2 days of work. 22:58 < kanzure> i think openworm is more accurate 22:58 < kirka> No nervous system yet, just physics 22:59 < kirka> kanzure Surely, you are right. I'm just playing 22:59 < kanzure> okay 22:59 < kanzure> have you ever used openworm? 23:00 < kirka> I'm following that project, and I have read papers, but it's still on early stages: there are just some tech demos and incomplete neural network somulator 23:02 < kirka> Openworm is a very interesting project 23:02 -!- kmo [122@unaffiliated/kmo] has joined ##hplusroadmap 23:03 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 264 seconds] 23:03 < kanzure> i think there's another simulator that you are skipping 23:03 -!- makoLime [~mako@103-9-42-128.flip.co.nz] has joined ##hplusroadmap 23:03 < kirka> Nemaload? Or Cyber Elegans? 23:03 -!- wibzra [~userdi@c-76-23-254-105.hsd1.ct.comcast.net] has quit [Ping timeout: 240 seconds] 23:03 < kanzure> not sure, sorry 23:04 < kirka> This inspired me https://www.youtube.com/watch?v=3uV3yTmUlgo 23:06 < kirka> They have model of c.elegans neural network, but they cannot simulate it because of lack of synaptic weights 23:06 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 23:08 -!- makoLime [~mako@103-9-42-128.flip.co.nz] has quit [Ping timeout: 264 seconds] 23:13 -!- ParahSailin [~Rob@unaffiliated/parahsailin] has quit [Read error: Connection reset by peer] 23:14 < kirka> kanzure Is there any list of DIYbio achievements? I mean successful transformation of organisms and other projects. 23:16 < kanzure> there's http://diyhpl.us/wiki/diybio/faq/news 23:16 < kanzure> but no there's only http://diyhpl.us/wiki/diybio/faq 23:21 -!- makoLime [~mako@103-9-42-128.flip.co.nz] has joined ##hplusroadmap 23:30 < kirka> Looks interesting http://www.bio-rad.com/prd/en/US/LSE/PDP/619b8f74-9d3f-4c2f-a795-8a27e67598b7/pGLO-Bacterial-Transformation-Kit 23:32 < kirka> But transformation isn't easy 23:32 < kanzure> it's easy compared to other projects. 23:32 < kirka> I should learn to culture ecoli first 23:36 -!- AlonzoTG [~atg@pool-108-28-74-221.washdc.fios.verizon.net] has quit [Ping timeout: 252 seconds] 23:36 < kirka> So, I'll have to build an incubator with thermostat, and I'll need something for heat-shocking ecoli 23:37 -!- AlonzoTG [~atg@pool-108-28-74-221.washdc.fios.verizon.net] has joined ##hplusroadmap 23:38 < kirka> Heat shock is done in water bath 23:39 < kanzure> you could also use electroporation 23:40 < yashgaroth> heat shock is easier 23:41 < kirka> I understand. I'm thinking of simply DIUbio home lab setup. There is some information https://groups.google.com/forum/?fromgroups=#!topic/diybio/FlrxI3JcniE 23:41 < kanzure> what is "DIU" 23:41 < kanzure> oh a typo 23:41 < kirka> DIY mistyped 23:42 < kanzure> nevermind 23:45 -!- ParahSailin [~Rob@50-194-178-148-static.hfc.comcastbusiness.net] has joined ##hplusroadmap 23:47 < kirka> So, for that experiment http://www.bio-rad.com/webroot/web/images/lse/products/pglo_gfp_kits/product_overlay_content/global/pglo_bac_trans_kit_flowchart.jpg I'll need incubator and water bath. For doing simple experiments like this one making my own agar plates will be useful. Also I'll have to sterilize petri dishes before incubation. Is that enough for transformation? 23:54 < yashgaroth> you don't necessarily need a waterbath, just a thermometer 23:55 < yashgaroth> are you just gonna buy the kit and do that or something 23:56 < kirka> Ok. 23:57 < kirka> What was your first project in diybio? 23:58 < yashgaroth> who, me? 23:58 < kirka> you and kanzure 23:59 < yashgaroth> I do bacterial transformations at work, I don't really bother replicating that at home --- Log closed Tue May 21 00:00:29 2013