--- Log opened Tue Jul 02 00:00:13 2013 00:01 -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 00:05 -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Ping timeout: 240 seconds] 00:40 -!- monkeynipples_ [monkeynipp@gateway/shell/xzibition.com/x-yicirlggpkeehflv] has joined ##hplusroadmap 00:40 -!- monkeynipples [monkeynipp@gateway/shell/xzibition.com/x-rfdxwsuzmjptbgvz] has quit [Ping timeout: 240 seconds] 01:19 < nmz787_> paperbot: http://journals.sfu.ca/rncsb/index.php/csbj/article/download/csbj.201304003/271 01:19 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/f2c5005dd99d30dd7f38e07214cb91b0.pdf 01:19 < nmz787_> 'The biological microprocessor, or how to build a computer with 01:19 < nmz787_> biological parts' 01:20 < nmz787_> "Systemics, a revolutionary paradigm shift in scientific thinking, with applications in systems biology, and synthetic 01:20 < nmz787_> biology, have led to the idea of using silicon computers and their engineering principles as a blueprint for the engineering of a 01:20 < nmz787_> similar machine made from biological parts. Here we describe these building blocks and how they can be assembled to a general 01:20 < nmz787_> purpose computer system, a biological microprocessor. Such a system consists of biological parts building an input / output 01:20 < nmz787_> device, an arithmetic logic unit, a control unit, memory, and wires (busses) to interconnect these components. A biocomputer can 01:20 < nmz787_> be used to monitor and control a biological system." 01:37 -!- Juul [~Juul@208.87.217.74] has quit [Ping timeout: 252 seconds] 01:47 -!- Juul [~Juul@208.87.217.74] has joined ##hplusroadmap 02:25 -!- Juul [~Juul@208.87.217.74] has quit [Ping timeout: 246 seconds] 02:32 -!- eleitl [~eugen@v8.ativel.com] has joined ##hplusroadmap 02:33 < eleitl> paperbot: http://dx.doi.org/10.1016/j.theriogenology.2008.11.005 02:33 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/9a4cf4bf851a85a22d956075bbb6c77c.txt 02:34 <@archels> hoi eleitl 02:35 <@archels> http://turingbirds.com/temp/1-s2.0-S0093691X08007784-main.pdf 02:35 < eleitl> Hi archels -- can you access that journal? 02:36 < eleitl> Ah, that was just a test paper. 02:36 < eleitl> Paperbot doesn't seem to be able to. 02:37 <@archels> yep, I have access 02:37 < eleitl> Institutional? 02:38 <@archels> nod 02:38 < eleitl> As a matter of personal favor, I might have to ask you for some articles in future. 02:38 <@archels> sure 02:39 -!- Charlie [~quassel@74.63.212.44] has quit [Ping timeout: 256 seconds] 02:39 < eleitl> We're about to form a startup, and evaluating what particular biological system to cryopreserve. 02:40 <@archels> this is an offshoot of that lab space you guys were setting up? 02:40 <@archels> I thought you were working on cryopreserving L. stagnalis 02:40 < eleitl> Yes. 02:42 < eleitl> Sorry, phone. 02:42 < eleitl> No, the L. stagnalis is a project which is difficult to get investors to pay for. 02:42 < eleitl> We'll probably try to weaselt that in via way of nonprofit, via donations. 02:43 < eleitl> We have a first internal round of investors which are interested in human cryopreservation, but can't pay for it all, especially if it's not a sustainable business. 02:44 < eleitl> First funding round is small just about 55 kEUR, for the first 12 months. 02:44 <@archels> ah. This is focused at advancing the necessary technology, rather than actually setting up long-term human cryo storage? 02:45 < eleitl> We do aim for a provider in Switzerland, but that's more long-term. 02:46 < eleitl> Our first priority is to start cryopreserving something already useful, and produce enough results to be able to raise a lot more money in the second round. 02:47 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 02:47 < eleitl> We're investigating several potential candidates, but unfortunately this is all for-profit business plan, so hush-hush. 02:48 <@archels> I guess the return-on-investment on fundamental research cannot be expected to be very high 02:48 < eleitl> It's more a teaser, to raise serious cash in the second round. 02:48 <@archels> right 02:49 < eleitl> We do have some interesting candidates, but research is intrinsically uncertain. You can work 1-2 years and only run into a wall. 02:49 < eleitl> We're going to have a specific exit clause in the contract in case we run into a wall. 02:50 <@archels> sure, but cryopreservating is already a commercially successful business, so you're not starting from square one 02:50 <@archels> unless you're planning on doing something radically different 02:50 < eleitl> yes, we're trying to improve existing methods, or we're trying to do something reasonably radical 02:51 < eleitl> so we'll spend next 2-3 months reading papers, trying to figure out where our chances are better 02:51 < eleitl> currently there are 3 options, ideally I want to have a few more 02:52 < eleitl> what would you try to do if you were me? 02:53 <@archels> beats me, I have no intuition for business =) 02:54 <@archels> generally I would argue for doing one thing and doing it well, but if you're just working up towards attracting more capital, then doing a few things in parallel could be beneficial 02:55 <@archels> because it gives you more things to show to potential investors 02:55 < eleitl> we will be focusing on one thing, but we try it to be the right thing 02:56 < eleitl> if you want to raise, say 1-10 MEUR in the next round it better be something slightly exciting 02:59 <@archels> not sure what is a reasonable target given 55k€ and 12 months 03:00 < eleitl> I hesitate to say more, as archives are public 03:01 < eleitl> we have something hot, but quite uncertain 03:01 < eleitl> if we go that route, 12 months will be just getting competent with that system 03:01 < eleitl> if results are not a total failure, you can prolong the phase, or up the ante 03:02 < eleitl> it might make sense to become a licensed animal research facility 03:03 < eleitl> I understand it's easier if you order animals from a commercial supplier, and don't hold them longer than 12 hours in your research facility 03:03 < eleitl> this is US law, it might be different in the EU 03:03 < eleitl> slaughterhouse is easy, but not really reproducible 03:07 <@archels> hum, how fresh is the tissue you can get from there, anyway? 03:08 < eleitl> if you pick it up yourself, very fresh 03:09 <@archels> hope you have one nearby the lab, then :) 03:09 <@fenn> cats and dogs seems like a reasonable place to start; there's demand for it after all 03:09 <@fenn> mice are convenient 03:10 < eleitl> in case of slaughterhouse, it will be pigs and cows 03:10 <@fenn> but they are so big 03:10 < eleitl> in case of commercial animals, rabbits or tats 03:10 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 03:10 < eleitl> big is a plus, actually 03:12 <@archels> .wa volume of pig brain 03:12 < yoleaux> pig brain: amount: 3 oz (ounces): serving volume: (data not available); Physical properties: mass: 3 oz (ounces) 03:12 <@archels> canhazmetric? 03:12 <@fenn> yum yum 03:12 <@fenn> that was serving size from a food database 03:12 < eleitl> unfortunately, brains are not transplanted, and slaughterhouse methods tend to ruin your brain 03:13 < eleitl> removing the brain is also very difficult, and time-consuming 03:14 <@fenn> i dont suppose you could get dead dolphins 03:14 < eleitl> even fixated brains are difficult, needle-nose pliers removing pieces of skull, etc. 03:14 -!- Charlie [~quassel@74.63.212.44] has joined ##hplusroadmap 03:14 < eleitl> I see no point in dolphins, and in general I'd rather stay away from the smarter critters 03:14 <@archels> so pigs about 0.1L, compared to human ~1L? 03:15 <@archels> dolphins, haha 03:15 < eleitl> in case of brains, I would still like to look at snails 03:15 < eleitl> but, sales proposition to investors: zero 03:16 < eleitl> ditto whole body transplants, for that matter 03:16 <@fenn> looks like a pig brain is 180g 03:16 < eleitl> I'd hate working with live pigs 03:17 <@fenn> from http://mste.illinois.edu/malcz/DATA/BIOLOGY/Animals.html 03:17 <@fenn> mountain beaver? 03:17 <@archels> eleitl: I take it you've considered rats? 03:18 <@archels> not sure what you're trying to do; if working on perfusion methods then small brains like these are probably useless 03:18 < eleitl> yes, in case we go animal research route rats and rabbits 03:18 < eleitl> but, first year we'll be doing fresh cadaver tissue 03:18 < eleitl> strictly no live critters on the premises 03:18 <@archels> except for the scientists 03:19 < eleitl> was thinking that as well ;) 03:19 < eleitl> human research is less regulated, probably 03:19 < eleitl> sacrifice a few lab assistants... 03:19 < eleitl> nobody going to notice... 03:19 < gradstudentbot> Have you read this paper? 03:21 <@archels> I think we got ourselves a volunteer 03:26 < eleitl> codo derecho se objetiva fractura de cupula radial <- fracture of the radial head 03:26 < eleitl> correct? 03:26 < eleitl> http://qz.com/99413/first-ever-human-head-transplant-is-now-possible-says-neuroscientist/ 03:30 < heath> http://www.bu.edu/abl/publications.html 03:30 < heath> .title 03:30 < yoleaux> Boston University: Collins Lab 03:30 < heath> publications from the lab 03:32 -!- Viper168_ [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 03:33 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 252 seconds] 03:39 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 03:40 -!- Charlie [~quassel@74.63.212.44] has quit [Ping timeout: 252 seconds] 04:15 < nmz787_> paperbot: http://www.sciamdigital.com/index.cfm?fa=Products.ViewIssuePreview&ISSUEID_CHAR=E94E8DBF-C927-4DCD-B32C-221B7832ABB&ARTICLEID_CHAR=AB980A62-605D-4562-8CA1-360E43FA131 04:15 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/dfa8eec7860d311a26224758562e0d10.txt 04:21 < nmz787_> paperbot: http://solidstate.um.ac.ir/parameters/solidstate/filemanager/Scientific.American.Special.Edition.1997-12_Solid_State_Century.pdf 04:24 < paperbot> ConnectionError: [Errno 110] Connection timed out (file "/usr/local/lib/python2.7/dist-packages/requests/models.py", line 625, in send) 04:41 -!- Viper168_ [~Viper@unaffiliated/viper168] has quit [Ping timeout: 256 seconds] 04:47 -!- Charlie [~quassel@74.63.212.44] has joined ##hplusroadmap 04:54 -!- makoLime [~mako@103-9-42-152.flip.co.nz] has quit [Read error: Operation timed out] 05:07 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has joined ##hplusroadmap 05:12 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 05:12 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Remote host closed the connection] 05:15 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 05:42 -!- archels [~neuralnet@sascha.esrac.ele.tue.nl] has quit [Changing host] 05:42 -!- archels [~neuralnet@unaffiliated/archels] has joined ##hplusroadmap 05:42 -!- ServerMode/##hplusroadmap [+o archels] by pratchett.freenode.net 05:42 -!- mode/##hplusroadmap [-o archels] by ChanServ 05:49 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 256 seconds] 06:10 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 06:12 -!- yorick [~yorick@oftn/member/yorick] has joined ##hplusroadmap 06:21 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 260 seconds] 06:34 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 06:55 -!- marciogm [~textual@177.106.60.46] has joined ##hplusroadmap 07:03 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Ping timeout: 252 seconds] 07:06 -!- marciogm [~textual@177.106.60.46] has quit [Ping timeout: 276 seconds] 07:07 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 07:15 -!- Gue______ [~textual@177.106.60.46] has joined ##hplusroadmap 07:16 -!- Gue______ is now known as marciogm 07:16 -!- marciogm [~textual@177.106.60.46] has quit [Changing host] 07:16 -!- marciogm [~textual@unaffiliated/marciogm] has joined ##hplusroadmap 07:22 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 264 seconds] 07:34 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 07:56 -!- ianmathwiz7 [~chatzilla@ip65-46-170-6.z170-46-65.customer.algx.net] has joined ##hplusroadmap 08:14 -!- kajetan [122@unaffiliated/kmo] has quit [Remote host closed the connection] 08:22 -!- rk[zZz] is now known as ryankarason 08:22 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 246 seconds] 08:35 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 08:37 <@ParahSailin> wasted 15 minutes because i pasted a command where - was replaced by – 08:38 <@ParahSailin> fucking code point U0x2013 08:40 < chris_99> yeah that's a PITA 08:41 <@kanzure> it seems my phone is unable to charge unless i am directly staring at it 08:41 <@kanzure> something about the micro usb connection not making full contact when i am not looking 08:44 <@kanzure> and rebooting changes the charge from 2% to 40% 08:49 -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 08:54 -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Ping timeout: 256 seconds] 09:10 -!- yorick [~yorick@oftn/member/yorick] has quit [Remote host closed the connection] 09:22 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 240 seconds] 09:26 < balrog> kanzure: ya there? 09:27 <@kanzure> maybe 09:27 < balrog> wanna add support for IOP to pdfparanoia? 09:27 < balrog> I can provide a sample 09:27 <@kanzure> balrog: https://github.com/kanzure/pdfparanoia 09:28 <@kanzure> i think iop is already supported 09:28 <@kanzure> or there is an open bug report 09:28 < balrog> also the version in pip is busted 09:28 <@kanzure> ah, iop is not supported 09:28 <@kanzure> why is it busted? 09:28 < balrog> --verbose doesn't work 09:28 < balrog> -o doesn't work 09:28 <@kanzure> can you fix? :( 09:28 <@kanzure> i am really busy right now 09:28 < balrog> it doesn't specify pdfminer as a prereq 09:28 < balrog> ah :| 09:28 <@kanzure> pdfminer is not a prerequisite 09:29 <@kanzure> oh wait, it is 09:29 <@kanzure> https://github.com/kanzure/pdfparanoia/blob/master/requirements.txt 09:30 < balrog> IOP seems to be "strip first page if contains certain text" 09:30 < balrog> of course there may be additional watermarks 09:31 <@kanzure> sure 09:31 <@kanzure> one of the other plugins does that right now 09:31 < gradstudentbot> Friends don't let friends go to super school. 09:31 <@kanzure> but it has a lot of checks 09:31 < balrog> alternatively the ip and timestamp could be removed 09:31 <@kanzure> because stripping the first page when it's not iop would be deadly 09:33 -!- marciogm [~textual@unaffiliated/marciogm] has quit [Quit: Computer has gone to sleep.] 09:36 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 09:39 < balrog> I wonder if a becton-dickinson facscalibur PCI interface card would be of use to anyone 09:39 < balrog> I don't have any other hardware or software 09:49 -!- ianmathwiz7 [~chatzilla@ip65-46-170-6.z170-46-65.customer.algx.net] has quit [Ping timeout: 240 seconds] 09:50 -!- pads [~not@100.43.114.90] has quit [Ping timeout: 268 seconds] 09:54 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 09:59 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Ping timeout: 256 seconds] 09:59 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has quit [Read error: Connection reset by peer] 10:00 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has joined ##hplusroadmap 10:03 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 10:24 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 276 seconds] 10:36 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 11:13 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 276 seconds] 11:22 -!- EnLilaSko- [~Nattzor@host-85-30-145-65.sydskane.nu] has joined ##hplusroadmap 11:23 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has quit [Ping timeout: 256 seconds] 11:24 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 256 seconds] 11:24 -!- delinquentme [~asdfasdf@66.233.132.203] has joined ##hplusroadmap 11:25 <@ParahSailin> any way to write "def derp(x): return (x, dict())" as a lambda or is this just language smell 11:27 <@kanzure> [(x, dict()) for x in whatever] 11:28 <@kanzure> oops, that's a list comprehension 11:28 < brownies> heh 11:28 < brownies> i think you have to be careful about when the dict() gets evaluated... 11:30 <@ParahSailin> yeah thats the referential transparency thing that python lacks 11:32 <@ParahSailin> is there list comprehension sugar for making itertools generators? 11:37 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 11:56 -!- ThomasEgi [~thomas@185.5.8.81] has joined ##hplusroadmap 11:56 -!- ThomasEgi [~thomas@185.5.8.81] has quit [Changing host] 11:56 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 11:58 -!- EnLilaSko- is now known as EnLilaSko 11:59 -!- EnLilaSko [~Nattzor@host-85-30-145-65.sydskane.nu] has quit [Changing host] 11:59 -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap 12:01 < delinquentme> kanzure, EYE MISS YEWWWW 12:09 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Read error: Connection reset by peer] 12:14 -!- padz [~not@100.43.114.90] has joined ##hplusroadmap 12:22 -!- cogitokat [~kat@ip70-171-6-179.ga.at.cox.net] has quit [Quit: Lost terminal] 12:22 -!- marciogm [~textual@177.106.60.46] has joined ##hplusroadmap 12:24 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 240 seconds] 12:29 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 12:33 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 12:37 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 12:44 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Quit: Leaving] 12:44 -!- delinquentme [~asdfasdf@66.233.132.203] has quit [Ping timeout: 252 seconds] 12:57 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 12:59 <@ParahSailin> coworker is using ^I's for tabs instead of spaces; told to help him with project; time to quit 13:05 <@ParahSailin> repo master refuses to learn what pull request is on github; have to email diffs; time to quit 13:07 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Remote host closed the connection] 13:10 <@kanzure> ParahSailin: why are you bothering 13:10 <@kanzure> "Jonathan Rothberg has resigned from Life Technologies, amid Life's attempted $13.6 billion merger with Thermo Fisher Scientific. 13:10 <@kanzure> Rothberg's Ion Torrent business unit was cited by Thermo as a motivating factor behind the acquisition." 13:11 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 13:16 -!- kmo [~kmo@apn-46-169-138-228.dynamic.gprs.plus.pl] has joined ##hplusroadmap 13:16 -!- kmo [~kmo@apn-46-169-138-228.dynamic.gprs.plus.pl] has quit [Changing host] 13:16 -!- kmo [~kmo@unaffiliated/kmo] has joined ##hplusroadmap 13:20 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Quit: Leaving] 13:20 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 13:25 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 264 seconds] 13:37 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 13:42 -!- makoLime [~mako@103-9-42-152.flip.co.nz] has joined ##hplusroadmap 13:57 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 268 seconds] 14:07 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Read error: Connection reset by peer] 14:09 < Burninate> http://www.surgicalneurologyint.com/article.asp?issn=2152-7806;year=2013;volume=4;issue=2;spage=335;epage=342;aulast=Canavero;type=0 14:11 < Burninate> so Goertzel lives near me 0.o 14:12 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 14:15 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Client Quit] 14:19 <@kanzure> Burninate: i think he has a few kids. one is probably still in high school. 14:20 < Burninate> his face looks like someone I know the first name of, but not hte last 14:20 < Burninate> in college 14:20 < Burninate> *just graduated I guess 14:23 <@kanzure> not the amelious zarathustra one 14:23 <@kanzure> the other one 14:24 -!- padz [~not@100.43.114.90] has quit [Read error: Connection reset by peer] 14:25 -!- padz [~not@100.43.114.90] has joined ##hplusroadmap 14:26 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 252 seconds] 14:27 -!- smeaaagle [~smeaaagle@2002:6ca6:4fb1::6ca6:4fb1] has quit [Ping timeout: 256 seconds] 14:29 -!- smeaaagle [~smeaaagle@2002:6ca6:4fb1::6ca6:4fb1] has joined ##hplusroadmap 14:36 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 14:38 -!- Reverse_dog [~I@c-71-201-142-136.hsd1.il.comcast.net] has joined ##hplusroadmap 14:40 <@kanzure> Reverse_dog: hi 14:40 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 14:40 <@kanzure> does anyone have recommendations for which sdr things to buy? 14:42 <@kanzure> maybe i should also pick something out from http://www.aircrack-ng.org/doku.php?id=compatibility_drivers 14:50 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Remote host closed the connection] 14:50 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 14:55 -!- klafka [~klafka@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Ping timeout: 264 seconds] 15:06 < jrayhawk_> broadcasting or receiving? 15:07 <@kanzure> how about both! 15:07 <@kanzure> i just feel morally bad that i do not have this hardware laying around 15:10 <@kanzure> i expect superkuh to have an idea or two except for whatever reason he is not actually here 15:12 < chris_99> bladerf looks pretty nifty to me 15:14 -!- Reverse_dog [~I@c-71-201-142-136.hsd1.il.comcast.net] has quit [Quit: Leaving] 15:18 -!- Juul [~Juul@static.3.202.4.46.clients.your-server.de] has joined ##hplusroadmap 15:23 -!- phillyj [~Thunderbi@c-68-80-251-160.hsd1.pa.comcast.net] has joined ##hplusroadmap 15:25 -!- weles [~mariusz@c-71-234-3-169.hsd1.ct.comcast.net] has joined ##hplusroadmap 15:27 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 252 seconds] 15:38 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 15:43 -!- pads [~not@100.43.114.90] has joined ##hplusroadmap 15:44 -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 15:45 -!- yorick [~yorick@oftn/member/yorick] has joined ##hplusroadmap 15:45 -!- padz [~not@100.43.114.90] has quit [Ping timeout: 246 seconds] 15:48 -!- ParahSailin_ [~ropoctl@99-25-202-211.lightspeed.hstntx.sbcglobal.net] has joined ##hplusroadmap 15:48 -!- ParahSailin_ [~ropoctl@99-25-202-211.lightspeed.hstntx.sbcglobal.net] has quit [Changing host] 15:48 -!- ParahSailin_ [~ropoctl@unaffiliated/parahsailin] has joined ##hplusroadmap 15:52 -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has quit [Remote host closed the connection] 16:01 -!- marciogm [~textual@177.106.60.46] has quit [Quit: Computer has gone to sleep.] 16:07 -!- phillyj [~Thunderbi@c-68-80-251-160.hsd1.pa.comcast.net] has quit [Quit: phillyj] 16:16 < balrog> what was that zotero auto-import plugin? 16:27 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 256 seconds] 16:29 <@kanzure> balrog: zotero translators 16:29 <@kanzure> balrog: https://github.com/zotero/translators 16:29 <@kanzure> balrog: https://github.com/zotero/translation-server 16:38 -!- ParahSailin_ [~ropoctl@unaffiliated/parahsailin] has quit [Quit: ParahSailin_] 16:39 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 16:49 -!- jrayhawk_ is now known as jrayhawk 17:00 -!- yorick [~yorick@oftn/member/yorick] has quit [Remote host closed the connection] 17:21 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 17:22 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 17:26 -!- joehot [~not@100.43.114.90] has joined ##hplusroadmap 17:28 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 246 seconds] 17:29 -!- pads [~not@100.43.114.90] has quit [Ping timeout: 256 seconds] 17:41 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 17:47 < superkuh> paperbot: http://proceedings.aip.org/resource/2/apcpcs/664/1/194_1?isAuthorized=no 17:47 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/Ablative%20Laser%20Propulsion%3A%20A%20Study%20of%20Specific%20Impulse%2C%20Thrust%20and%20Efficiency.txt 17:47 <@kanzure> i wonder if i am stripping out isAuthorized=no 17:48 < superkuh> paperbot: http://proceedings.aip.org/resource/2/apcpcs/664/1/194_1 17:48 < paperbot> error: HTTP 500 http://diyhpl.us/~bryan/papers2/paperbot/Ablative%20Laser%20Propulsion%3A%20A%20Study%20of%20Specific%20Impulse%2C%20Thrust%20and%20Efficiency.txt 17:48 < superkuh> paperbot: http://scitation.aip.org/getpdf/servlet/GetPDFServlet?filetype=pdf&id=APCPCS000664000001000194000001&idtype=cvips&doi=10.1063/1.1582108&prog=normal 17:48 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/User%20Authentication.txt 17:48 <@kanzure> archels: maybe you have access? 17:49 < gradstudentbot> I have to read all these articles. 17:52 -!- Juul [~Juul@static.3.202.4.46.clients.your-server.de] has quit [Ping timeout: 252 seconds] 17:53 -!- balrog [~balrog@discferret/developer/balrog] has quit [Ping timeout: 256 seconds] 17:56 < streety> I think some selection process would be necessary 17:57 -!- Charlie_ [~quassel@74.63.212.44] has joined ##hplusroadmap 17:57 -!- Charlie [~quassel@74.63.212.44] has quit [Write error: Connection reset by peer] 17:57 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Excess Flood] 17:57 -!- Charlie_ is now known as Guest61414 17:58 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 17:58 -!- balrog [~balrog@discferret/developer/balrog] has joined ##hplusroadmap 17:59 -!- yashgaroth [~ffffff@cpe-66-27-118-94.san.res.rr.com] has joined ##hplusroadmap 18:00 -!- padz [~not@100.43.114.90] has joined ##hplusroadmap 18:01 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has quit [Ping timeout: 246 seconds] 18:01 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has joined ##hplusroadmap 18:01 -!- joehot [~not@100.43.114.90] has quit [Ping timeout: 246 seconds] 18:01 -!- klafka_ [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 18:02 -!- hehelleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap 18:10 -!- Netsplit *.net <-> *.split quits: gradstudentbot, helleshin, klafka 18:11 -!- Netsplit over, joins: gradstudentbot 18:18 < jonathan__> how do I explain genetic algorithm seo optimization in order to pick the name of a new product, to a friend of mine who knows absolutely nothing about math or science and also thinks their opinion is usually the best...? 18:19 < jonathan__> it's like explaining evolution to a catholic priest.. 18:29 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 276 seconds] 18:36 <@kanzure> jonathan__: i don't think genetic algorithms are the best way to do seo optimization. you should start from the other-end of the spectrum by looking at google search keyword tools that tells you how many people search for a given term. 18:36 < jonathan__> sure, same thing 18:37 < jonathan__> the reply I got back was something like "well I like my name better so what does it matter what other people think" ... ummm yea 18:38 < jonathan__> google adwords is just a manual simplistic way of doing genetic algorithm really 18:42 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 18:43 <@kanzure> google adwords forces you to increase your margins so you can dump more of it into adwords 18:48 -!- Urchin[emacs] [~user@unaffiliated/urchin] has quit [Ping timeout: 264 seconds] 19:05 -!- Juul [~Juul@static.3.202.4.46.clients.your-server.de] has joined ##hplusroadmap 19:23 -!- Urchin[emacs] [~user@unaffiliated/urchin] has joined ##hplusroadmap 19:27 -!- pads [~not@100.43.114.90] has joined ##hplusroadmap 19:29 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 276 seconds] 19:30 -!- padz [~not@100.43.114.90] has quit [Ping timeout: 240 seconds] 19:34 -!- ianmathwiz7 [~chatzilla@68-115-57-210.static.eucl.wi.charter.com] has joined ##hplusroadmap 19:41 < jonathan__> oh, like cocaine. "cocaine makes me work better. so I can make more money. so I can buy more cocaine. so I can work better. so I can make more money. so I can buy more cocaine. ..." 19:41 -!- ianmathwiz7 [~chatzilla@68-115-57-210.static.eucl.wi.charter.com] has quit [Quit: ChatZilla 0.9.90 [SeaMonkey 2.17.1/20130410205058]] 19:41 < jonathan__> angelina jolie will now remove her ovaries to prevent future cancer. ... 19:42 < jonathan__> so what body parts can I remove to reduce my cancer risk? 19:43 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 19:43 < yashgaroth> three, but you're not gonna like it 20:02 < jonathan__> oh, says who? maybe I enjoy self mutilation 20:03 < jonathan__> let me see, my esophogus? 20:04 -!- rk[imposter] [~rkimposte@stallman.cse.ohio-state.edu] has joined ##hplusroadmap 20:05 < yashgaroth> hell if you go on soylent you can safely remove most of your digestive tract 20:26 < jonathan__> i hope that stuff is tasty 20:26 < jonathan__> i saw they were hiring for lab techs 20:27 < jonathan__> to make the stuff.. 20:27 < jonathan__> yum! cant wait to have my soylent chicken mcnuggets 20:27 < yashgaroth> 'lab tech' is a little optimistic 20:28 < jonathan__> ok, factory worker 20:28 < yashgaroth> artisan manual labor 20:29 < jonathan__> not so arty artisan 20:29 < jonathan__> glorified stirring rod ok 20:29 < yashgaroth> I'm waiting for the choco-soylent 20:30 < jonathan__> speaking of job opps I've now seen cybercoder ad for lead android engineer, listed $130K-$200K DOE 20:30 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 252 seconds] 20:30 < jonathan__> now thats what i'm talkin bout yo 20:30 < jonathan__> maybe that $200K is fake tho, I dunno 20:30 < jonathan__> I dunno about soylent but I would love some hemp burger 20:31 < yashgaroth> mm, lentil steak 20:31 < jonathan__> so there was a lab meetup today at 1pm 20:31 < yashgaroth> damn I was busy for some reason 20:31 < jonathan__> I was there at 1pm with some dude who came in off the street to help 20:32 < jonathan__> but.... no you know who 20:32 < yashgaroth> like literally just walked in? 20:32 < jonathan__> new dude was basically sitting around staring at the wall while I attempted to revive my babies 20:32 < jonathan__> some way to treat free labor :-( 20:32 < jonathan__> yea 20:33 < jonathan__> well there was a carpenter dude there working on lab benches so lab was unlocked 20:33 < yashgaroth> I went and saw it on sunday, new benches have a distinct lack of ergonomics for people with legs 20:33 < jonathan__> yea well, maybe you're just too tall ha 20:33 < jonathan__> evolve yourself 20:33 < yashgaroth> man you gotta put your knees somewhere 20:33 < jonathan__> this is the era of downsizing 20:34 < jonathan__> the lab will only be rented out to paraplegics 20:34 < yashgaroth> also the display rack of big test tubes with colored liquid inside 20:34 < yashgaroth> I should make some skittles vodka in some of those for the opening 20:34 < jonathan__> I'm gonna have a special treat for the opening day, wanna know what it is 20:35 < jonathan__> oh it will be so cool 20:35 < jonathan__> for me, anyway lol 20:35 -!- eudoxia [~eudoxia@r190-135-66-107.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 20:35 < yashgaroth> no don't tell me it'll be a surprise 20:35 < yashgaroth> yes 20:35 < jonathan__> since I'm still the only one with an experiment, is that right? 20:35 < yashgaroth> teeeechnically 20:35 < jonathan__> I mean, something actually living breathing bio with genes and stuff 20:36 < jonathan__> "lets have a computer with fold it" ummm come onnnnnn 20:36 < jonathan__> that must be the "beyond" in btnb.. like, beyond believability that it will fly 20:36 < yashgaroth> kinda hard to start experiments when we still can't order shit to the lab address 20:36 -!- Adifex [~Adifex@75-148-41-126-Colorado.hfc.comcastbusiness.net] has joined ##hplusroadmap 20:37 < jonathan__> geez kind of hard to get work done in a meetup to do work if there's no one to direct the work, eh? 20:37 < gradstudentbot> Dropped my sample. 20:37 < jonathan__> dropped the ball more like dude 20:37 < yashgaroth> yeah I mentioned to joseph that I'd be to the one next sunday and he was like 'oh yeah I just set up the repeating meetings' 20:38 < jonathan__> presumably there's a work list, not 20:38 < jonathan__> anyways I hope to turn a profit for the grand opening, that is MY measure 20:39 < yashgaroth> job 1: make sure the machines go 'bing!' in time for opening day 20:39 < yashgaroth> or was it 'ping!' damnit we're screwed 20:39 < jonathan__> i dunno what HIS measure is, maybe most number of rookies asking for help over and over? 20:39 < yashgaroth> wait are you gonna be selling wine at the opening or kombucha 20:39 < jonathan__> my job 1: measure progress via $$$ earned. period 20:40 < jonathan__> what do you think.. I'm a friggin CAPITALIST cause I live in the U.S.A. not commie china 20:40 < yashgaroth> it's not usually my first, but until we can actually support the lab I'm okay with that 20:41 < jonathan__> $$ just is a really good yardstick of success. 20:41 < jonathan__> in this country, anyways 20:41 < yashgaroth> in pretty much any country, including china these days 20:42 < jonathan__> not that the definition of success makes any sense. other than that's what people want at the time. 20:42 < jonathan__> the new dude today is apparently a yeast grower. 20:42 < jonathan__> currently at a brewing supply co. 20:42 < yashgaroth> maybe he just hadn't showered 20:43 < yashgaroth> oh 20:43 < jonathan__> he was... fascinated... by my (your) glassware 20:43 < jonathan__> it made me somehow understand his career choices. 20:43 < yashgaroth> brb buying lockbox 20:44 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 20:44 < jonathan__> gradstudentbot, do you know any hot postdocs? 20:44 < gradstudentbot> Yeah, but his project was so easy. 20:58 -!- weles [~mariusz@c-71-234-3-169.hsd1.ct.comcast.net] has quit [Ping timeout: 252 seconds] 21:05 -!- randallagordon [~randall@75-175-52-220.ptld.qwest.net] has quit [Ping timeout: 252 seconds] 21:07 -!- randallagordon [~randall@71-38-144-220.ptld.qwest.net] has joined ##hplusroadmap 21:10 -!- Juul [~Juul@static.3.202.4.46.clients.your-server.de] has quit [Ping timeout: 264 seconds] 21:13 -!- eudoxia [~eudoxia@r190-135-66-107.dialup.adsl.anteldata.net.uy] has quit [Quit: leaving] 21:21 -!- Juul [~Juul@199-188-193-9.PUBLIC.monkeybrains.net] has joined ##hplusroadmap 21:24 -!- ParahSailin [~Rob@50-194-178-148-static.hfc.comcastbusiness.net] has quit [Ping timeout: 256 seconds] 21:30 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 21:30 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 264 seconds] 21:37 -!- ParahSailin [~Rob@50-194-178-148-static.hfc.comcastbusiness.net] has joined ##hplusroadmap 21:42 < audy> <3 gradstudentbot 21:42 < gradstudentbot> Who has the latest version of the paper? 21:43 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 21:53 * brownies pokes gradstudentbot 21:53 < gradstudentbot> I think the centrifuge is broken. 21:53 < brownies> .meow 21:53 < yoleaux> http://moar.edgecats.net/cats/5NrgZ.gif 22:02 < heath> http://parts.igem.org/Part:BBa_R0051 22:02 < heath> what does the SS AND DS acronyms stand for? 22:02 < heath> i'm guessing DS = dna sequence? 22:02 < heath> ss = _ sequence 22:02 < yashgaroth> single and double stranded 22:03 < heath> um, that makes complete sense 22:03 * heath blushes' 22:04 < heath> yashgaroth: do you know what OR1 and OR2 stand for? 22:04 < ParahSail1n> occipital reticulin 1 and 2 22:05 < yashgaroth> binding sites for cI, apparently 22:05 < yashgaroth> or is that cl, damn alphabet 22:05 < yashgaroth> oh nope it's cI 22:06 < yashgaroth> so if the cI protein is being expressed in the cell, the promoter is repressed 22:08 < jonathan__> bryan - about that paper you asked about, ultrasonic rug delivery 22:09 < heath> ParahSail1n: if you don't mind me askin,g how'd you know that? i'm trying to learn to put together my first assembly of these parts 22:10 < heath> i'm reading the RFCs and also noticing that some of these parts aren't as well characterized as others have already pointed out 22:10 < ParahSail1n> er, i just bs'd 22:11 < ParahSail1n> yashgaroth's cro operon fits a lot better than my made up words 22:11 < yashgaroth> my response was actually true but y'know whatever ignore me 22:11 < jonathan__> I guess the question was about the driving circuitry. well Im not sure why it takes a whole paper to describe differential signaling, anyways, it is relatively straightforward what they are doing, there is no need for an FPGA etc etc 22:12 < ParahSail1n> in general, if you give two letters and a number for a gene, you're usually not going to have a useful time finding what that is without more context 22:12 < gradstudentbot> The paper got rejected. 22:12 < ParahSail1n> so i didnt even bother googling 22:13 < yashgaroth> I thought it was just one of those technically correct answers 22:13 < jonathan__> kanzure: so in essence just use a TI 6x DSP chip 22:14 < ParahSail1n> http://www.uniprot.org/uniprot/?query=or1&sort=score 170 pages of or1 to choose from 22:15 < jonathan__> the bigger problem is that the piezo element they created is 100% custom fabrication 22:15 < jonathan__> can't get that off the shelf 22:17 < jonathan__> also I thought that acoustic delivery systems had a habit of destroying whatever was being delivered 22:17 < jonathan__> there's huge pressures at play, at micro sizes 22:17 < heath> yashgaroth: heh, i'm sorry 22:19 < yashgaroth> no worries 22:19 < jonathan__> It's a pretty meaty paper so I'd have to give it a more careful reading. 22:19 < heath> slightly under the influence of things affecting my discernment :) 22:19 < jonathan__> I just skimmed around here & there. 22:23 < jonathan__> suppose you guys had 5 undergrads at your 100% disposal right now to work on whatever you want. What would you have them work on? 22:31 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 252 seconds] 22:33 < heath> depends on their qualifications 22:34 < jonathan__> I think I would have them work on cloning micheal jackson. 22:36 < heath> i'm wondering which papers are associated with this guy: http://parts.igem.org/wiki/index.php?title=Part:BBa_J45004 22:36 < heath> jonathan__: where are you located btw? 22:36 < heath> kanzure: you know of anyone else here that's in the southeast? 22:37 < jonathan__> i have no location, on a public channel 22:37 * heath thinks he recalls TX 22:37 < heath> http://www.amazon.com/Synthetic-Biology-Part-Volume-Characterization/dp/0123850754 22:38 < heath> .title 22:38 < yoleaux> Synthetic Biology, Part A, Volume 497: Methods for Part/Device Characterization and Chassis Engineering (Methods in Enzymology): Chris Voigt: 9780123850751: Amazon.com: Books 22:38 < heath> i want this 22:38 < heath> http://libgen.info/view.php?id=723808 22:38 < heath> that works 22:40 < jonathan__> oh, perfect, subject Техника 22:40 < jonathan__> Hm let me file this under Техника 22:43 < jonathan__> Техника: One morning, as Gregor Samsa was waking up from anxious dreams, he discovered that in his bed he had been changed into a monstrous verminous bug. He lay on his armour-hard back and saw, as he lifted his head up a little, his brown, arched abdomen divided up into rigid bow-like sections. From this height the blanket, just about ready to slide off completely, could hardly stay in place. His numerous legs, pitifull 22:43 < jonathan__> in comparison to the rest of his circumference, flickered helplessly before his eyes. 22:44 < jonathan__> That's one for the undergrads too. 22:44 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 22:44 < yashgaroth> very Техникаesque 22:45 < jonathan__> yes quite 22:48 -!- marciogm [~textual@177.106.60.46] has joined ##hplusroadmap 22:49 -!- joehot [~not@100.43.114.90] has joined ##hplusroadmap 22:49 < jonathan__> many of the supposed projects I come up with are fine for undergrads or postdocs and often PI's claim it is worth pursuing, but the grad students, man they are never believers 22:50 < jonathan__> ok maybe here's a better question 22:51 < gradstudentbot> You know they keep the mice in better conditoins than us. 22:51 < jonathan__> what should the undergrads have to be experienced with in order to be valuable 22:51 -!- pads [~not@100.43.114.90] has quit [Ping timeout: 256 seconds] 22:53 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has quit [Remote host closed the connection] 22:53 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has joined ##hplusroadmap 22:57 -!- marciogm [~textual@177.106.60.46] has quit [Quit: Computer has gone to sleep.] 23:01 < jonathan__> paperbot: http://www.wiley.com/WileyCDA/WileyTitle/productCd-1118610733.html 23:01 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/b8435a6877fa587c45d8c700aa10b471.txt 23:02 < jonathan__> max, I'm gonna need some of this http://www.abmgood.com/APOE-siRNA-Lentivectors-7-APOE.html 23:05 < yashgaroth> don't we all 23:07 < jonathan__> paperbot http://www.nature.com/nrg/journal/v3/n10/pdf/nrg908.pdf 23:07 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/a5e9fa2a7db16690a87bfcfcca66db31.pdf 23:08 < jonathan__> do you know if that is really viable? 23:08 < yashgaroth> it's not really 23:09 < yashgaroth> RNAi has a lot of problems, lentiviruses have a lot of them too 23:10 < jonathan__> "Abstract | RNA interference (RNAi) is a powerful approach for reducing expression of endogenously expressed proteins. It is widely used for biological applications and is being harnessed to silence mRNAs encoding pathogenic proteins for therapy. Various methods — including delivering RNA oligonucleotides and expressing RNAi triggers from viral vectors — have been developed for successful RNAi in cell culture and in vivo. 23:10 < jonathan__> Recently, RNAi-based gene silencing approaches have been demonstrated in humans, and ongoing clinical trials hold promise for treating fatal disorders or providing alternatives to traditional small molecule therapies. " 23:11 < jonathan__> so what's the problem? 23:11 < yashgaroth> mostly everything about it 23:11 < yashgaroth> people got all hyped because it was the first novel regulatory system discovered in a decade 23:12 < yashgaroth> to get use out of knocking down a gene, you'll probably need to knock it down in a significant fraction of the cells 23:12 < yashgaroth> low duration of expression 23:12 < yashgaroth> and viral vectors are usually attacked by the immune system 23:14 < yashgaroth> in a few years it may play an important part in select therapies 23:14 -!- Juul [~Juul@199-188-193-9.PUBLIC.monkeybrains.net] has quit [Ping timeout: 276 seconds] 23:15 < jonathan__> hmmmm 23:15 < yashgaroth> you will often find that people who bank their entire academic career on a specific niche will be biased toward its relevancy 23:16 < jonathan__> well sounds like regular injections are just required right 23:16 < jonathan__> you know, bioshock style 23:16 < yashgaroth> the advantage of gene therapy is theoretically that you only need one, or maybe a few injections 23:16 < yashgaroth> there's some promising work in morpholinos, which are synthetic RNAi's with modified backbones that give them a much better half-life 23:17 < yashgaroth> since the half-life of RNAi is absurdly short in vivo 23:17 < jonathan__> well suppose that problem is solved... lets say a microinjector is surgically sewn in like an insulin pump 23:18 < yashgaroth> not recommended to take viral gene therapy without immunosuppressants 23:18 < yashgaroth> if you don't get the dose you need, you've immunized yourself against the medicine 23:20 < yashgaroth> it's hard to find a condition that's easily treated by RNAi alone, it's not like you often have Bad Gene X that if you could only turn it off you'd be fine 23:21 < yashgaroth> usually you just have two shitty recessive versions of Normal Gene X 23:22 < jonathan__> paperbot http://www.tandfonline.com/doi/pdf/10.1081/E-EBAF-120043002 23:22 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/8a72c68cef5e51ac07e4bcffccce4b44.txt 23:22 < yashgaroth> "RNAi technology promising to create safe and effective models that may in fact reach the herds in the future" right 23:22 < jonathan__> paperbot http://www.tandfonline.com/doi/abs/10.1081/E-EBAF-120043002 23:23 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/d6bf4314aa10356701bbc9d4def39774.txt 23:24 < jonathan__> ah you're such a pessimist 23:25 < yashgaroth> nah just accurate 23:25 -!- Adifex [~Adifex@75-148-41-126-Colorado.hfc.comcastbusiness.net] has quit [Quit: Adifex] 23:26 < jonathan__> I read it in Nature, it has to be true! 23:26 < jonathan__> paperbot http://www.annualreviews.org/doi/pdf/10.1146/annurev-biophys-083012-130404 23:26 < paperbot> error: HTTP 200 http://diyhpl.us/~bryan/papers2/paperbot/b15aa335bb7be028b24757b21d4efe33.pdf 23:26 < yashgaroth> add that one to gradstudentbot's cache 23:26 < gradstudentbot> Hey, does anyone have an extra undergrad? 23:28 -!- kmo [~kmo@unaffiliated/kmo] has quit [Quit: Leaving] 23:28 < jonathan__> what happened to that journal supposed to be for publishing papers that don't work... 23:29 < yashgaroth> journal of negative results? same thing that happened to everyone going to open access journals...'well not everyone's doing it so I'll look bad by publishing there' 23:30 < yashgaroth> my impact factoru~~ 23:31 < yashgaroth> oh you published in JNR? you must be terrible at experiments, unlike me who burns all results that don't fit my worldview/grant 23:31 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has quit [Ping timeout: 252 seconds] 23:32 < jonathan__> hm 23:32 < yashgaroth> I exaggerate for comedic and pessimistic effect, but not by much 23:33 < jonathan__> "Use and abuse of RNAi to study mammalian gene function" 23:33 < jonathan__> WG Kaelin - Science, 2012 23:34 < yashgaroth> heh 23:35 < jonathan__> My poor APOE 23:35 < jonathan__> will never be silenced :-( 23:37 < jonathan__> paperbot http://www.nature.com/mt/journal/vaop/ncurrent/full/mt2013124a.html 23:37 < paperbot> HTTP 401 unauthorized http://www.nature.com/mt/journal/vaop/ncurrent/pdf/mt2013124a.pdf 23:38 < jonathan__> oh you just need to add NANO particles! 23:38 < jonathan__> "Biodegradable Lipids Enabling Rapidly Eliminated Lipid Nanoparticles for Systemic Delivery of RNAi Therapeutics" 23:40 < jonathan__> zzzzz 23:40 < yashgaroth> eurgh and also zzzz 23:42 -!- yashgaroth [~ffffff@cpe-66-27-118-94.san.res.rr.com] has quit [Quit: Leaving] 23:43 -!- lupfantomo [~lupfantom@24-159-24-229.dhcp.jcsn.tn.charter.com] has joined ##hplusroadmap 23:56 -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 23:58 -!- klafka_ [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Ping timeout: 256 seconds] --- Log closed Wed Jul 03 00:00:14 2013