--- Log opened Wed Jan 15 00:00:24 2014
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03:23 < venturecommunist> this looks useful http://www.clothocad.org/
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04:32 <@archels> http://aisb50.org/
04:32 <@archels> anyone going to this?
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07:45 < superkuh> paperbot: http://www.nature.com/neuro/journal/vaop/ncurrent/full/nn.3620.html
07:45 < paperbot> http://libgen.org/scimag/get.php?doi=10.1038%2Fnn.3620
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08:36 < solom> hi
08:36 < solom> What is the low hanging fruit of hplus?
08:36 < kanzure> programming
08:38 < kanzure> basic lab skills, like running a pipetting machine or spectrogramscopegraph
08:38 < solom> I was asking about things I can apply to myself
08:38 < solom> that are proven to work with most people
08:39 < kanzure> learning skills is the most effective way to make transhumanism-related technology happen
08:39 < kanzure> http://diyhpl.us/wiki/declaration/
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08:48 < solom> kanzure, http://diyhpl.us/wiki/nootropics/notes.txt
08:49 < solom> What nootropics do you personally use
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08:50 < solom> http://diyhpl.us/wiki/users/kanzure/bodybuilding-drugs/
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08:53 < ParahSailin> amphetamine is the most effective noot
08:54 < solom> Aren't they a bit dangerous?
08:57 < ThomasEgi> does gradstudent bot know about this? -> "Recently," Baker writes, "I learned of a governing principle known as Murphy's first law of biology. It states: 'Under any given set of environmental conditions an experimental animal behaves as it damn well pleases.'
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09:00 < ParahSailin> only dangerous is you're the kind afraid of becoming more than human
09:00 < chris_99> you guys all seen the trailer for transcendance ?
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09:23 < wallmani> ParahSailin: do have hany any recommendations on what amphetamines to use?
09:24 < ParahSailin> whatever you can get your hands on
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09:25 < wallmani> any non prescription ones i can get?
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09:28 < ParahSailin> have you considered bath salts?
09:29 < chris_99> ThomasEgi, got my transducers, and just ordered a sig gen :)
09:36 < ParahSailin> specifically, 4-fluoroamphetamine
10:32 < cthulu> you can order stuff from hong kong
10:32 < cthulu> modafinil, dexamphetamine
10:39 < wallmani> cthulu: any recommended vendors for dexamphetamine?
10:39 < wallmani> ParahSailin: aren't bath salts bad?
10:41 < ua> wallmani, "thats what they want you to think"
10:42 < ua> but yea if you dont know what youre doing its just like with everything
10:42 < wallmani> ah, i see
10:44 < cthulu> go to #reddit-nootropics for sources
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10:55 < kanzure> no reddit
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11:17 < ParahSailin> wallmani: but seriously if you are trying to be cheap about it, ethylphenidate is probably cheaper than even an indian pharmacy for ritalin
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11:52 < wallmani> i see
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15:59 < kanzure> this place has a very small selection of lab supplies but they claim to accept bitcoin http://www.geneandcell.com/
15:59 < kanzure> oh, it's john schloendorn...
16:00 < kanzure> well that's boring
16:05 < kanzure> i thought he was running that other molecular biology supply company too
16:05 < kanzure> hrmm i guess it doesn't say http://www.openbiotech.com/aboutus.asp
16:06 < kanzure> "Open Biotechnology, Inc. 3603 Haven Ave., Suite 1, Menlo Park, CA 94025" blargh who is it that lives here.. hrm..
16:06 < kanzure> Justin Rebo <justin@openbiotech.com>
16:06 < kanzure> John Schloendorn <john@openbiotech.com>
16:07 < kanzure> ParahSailin: what's the story about this.. john just happens to be doing the same thing under two names at the same time?
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16:27 < ParahSailin> i never knew the guy very well
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16:36 < kanzure> bummer. next time make something up and i'd be just as happy.
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20:00 < kanzure> https://github.com/gulpjs/gulp "By harnassing the power of node's streams you get fast builds that don't write intermediary files to disk."
20:00 < kanzure> show me more mad science
20:00 < kanzure> i thought seaport/fleet would do continuous deployment but i'm not completely sure
20:01 < kanzure> e.g. hooking up continuous builds to continuous deployment seems like a fun thing to do
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20:15 < kanzure> nmz787: https://npmjs.org/package/inflate-stream for inflate/deflate in pdfs
20:24 < kanzure> node stream visualization http://thlorenz.github.io/stream-viz/
21:22  * juri_ waits to hear from the police whether her favorite transhumanist just attempted suicide while on the phone with her or not.
21:22 < juri_> yep. things are great in my world.
21:22 < kanzure> if you tell me the location i might have people nearby
21:23 < juri_> boulder colerado.
21:23 < juri_> police have been dispatched.
21:25 < juri_> i've attempted suicide a half dozen times myself, so i don't fuck around. as they say in heart attack cases, time is tissue.
21:26 < juri_> as for why i'm someone's last call, i only chalk that up to the universe's sense of irony / being a bastard. this is not my first time.
21:27 < kanzure> there's john english <john@boulderes.com> from the open source hardware scene, but i haven't seen him in a while
21:27 < kanzure> is it amara?
21:27 < juri_> Free Hardware is a rough scene indeed.
21:27 < juri_> nope.
21:28 < kanzure> yeah i think amara left boulder a while ago. hrmm.
21:28 < kanzure> it's not like there's that many transhumanists anyway
21:29 < juri_> technically, i don't consider myself a transhumanist. i'm a singularist.
21:30 < juri_> now, don't ask me to define the difference. we could be here all night. ;)
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21:31 < kanzure> oh geeze now i have to cross-reference all the singularitarians in boulder, all 5 of them or something
21:31 < kanzure> hrmm
21:31 < juri_> singulitarians? they only eat things that come individually served? ;)
21:32 < kanzure> no.. http://wiki.lesswrong.com/wiki/Singularitarianism
21:32 < kanzure> hah "Yudkowsky has (perhaps facetiously) suggested that those adhering to the original activist stance relabel themselves the "Elder Singularitarians"."
21:32 < kanzure> classic eliezer
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21:34 < juri_> wait, dr sbaetzo isn't a real doctor? ;)
21:34 < gradstudentbot> Did you order the carbon nanotubes yet?
21:35 < juri_> No, i haven't. :(
21:36 < kanzure> ./buildwrappers.sh: line 50: ed: command not found
21:37 < kanzure> is it really possible that i don't have ed?
21:37 < kanzure> seems unlikely. remaining skeptical.
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21:39 < Urchin[emacs]> what are you using?
21:42 < kanzure> building swig things for opentransactions
21:42 < kanzure> apparently i did not have ed installed..
21:46 < venturecommunist> semweb interest group and financial crypto?
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21:47 < kanzure> swig is a wrapper generator
21:47 < kanzure> the semantic web stuff is unrelated and boring
21:48 < venturecommunist> i've been working with financial crypto built on http://json-ld.org
21:48 < venturecommunist> http://web-payments.org
21:49 < kanzure> that's quite a lot of words
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21:50 < venturecommunist> words as opposed to?
21:50 < kanzure> specs, code, etc.
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21:50 < kanzure> this does not seem very compelling https://web-payments.org/specs/source/web-commerce/
21:50 < kanzure> yeah let's have a pile of unsigned transactions in json.. perfect?
21:51 < venturecommunist> signatures https://web-payments.org/specs/source/http-signatures/
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21:54 < venturecommunist> you found some specs clearly. here's some of my code https://github.com/venturecommunism/meteor-httpkeys/blob/master/server/httpsig.js
21:54 < venturecommunist> so there's specs and code
21:56 < kanzure> if(Meteor.isServer) {}
21:56 < kanzure> is that really how meteor does server/client side separation
21:56 < gradstudentbot> Oh man, that's a great scrabble word. I got to write that down.
21:58 < venturecommunist> kanzure: i just keep server code in the server folder
21:58 < juri_> ok, friend is fine.
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22:08 < venturecommunist> anyone have a dna assembly method they've used for fusion proteins that has minimal equipment requirements?
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22:13 < kanzure> what's wrong with in vivo protein construction..?
22:14 < venturecommunist> kanzure: you mean with no transformation step at all?
22:14 < venturecommunist> i didn't know you could make a predictable fusion protein thatway
22:15 < venturecommunist> or do you just mean "what's wrong with doing nothing"
22:16 < kanzure> dna templated assembly just seems like a huge hack around just doing normal molecular biology techniques
22:16 < kanzure> "let's use dna folding and stitches to make the proteins" is not something that biologists smile about every morning
22:18 < venturecommunist> so are there specs and protocols for this?
22:19 < venturecommunist> i don't know i've come across this yet on openwetware
22:19 < kanzure> the dna origami-guided protein assembly stuff? there's probably a stupid paper somewhere
22:19 < kanzure> openwetware is not a great way to learn about traditional molecular biology
22:19 < kanzure> except maybe finding a protocol or two sometimes
22:19 < kanzure> start here, http://diyhpl.us/~bryan/papers2/bio/books/Molecular%20Cloning%20-%20A%20Laboratory%20Manual%20-%20Sambrook%20-%203rd%20edition.pdf
22:22 < venturecommunist> k thanks i'll read this
22:23 < venturecommunist> kanzure: does this fall under DNA synthesis then?
22:24 < venturecommunist> i don't know if that's a loose or specific term but you couldn't specify base pairs from scratch like this could you?
22:24 < kanzure> you can sometimes use dna synthesis to write entire genes but often it's extremely expensive
22:24 < kanzure> well, sorry, no, average gene size is short, so it's not often expensive, but the fun stuff is at least $5k/protein or something
22:24 < venturecommunist> right which is why i haven't learned much about it
22:24 < kanzure> http://diyhpl.us/wiki/dna-synthesis.html
22:24 < venturecommunist> so this has transformation and plasmids in it too
22:25 < venturecommunist> what makes molecular cloning and dna assembly distinct categories?
22:26 < kanzure> are you talking about this sort of dna assembly? http://dna.caltech.edu/DNAresearch_publications.html
22:28 < venturecommunist> that looks more advanced. i'm definitely talking miniprep, PCR, heat shock transformation, etc
22:28 < venturecommunist> minimal equipment would be my ideal, with the goal of making fusion proteins
22:28 < venturecommunist> specifically HIV1 TAT + PKR + APAF1
22:28 < kanzure> then why do you keep saying dna assembly.. what is that if it's not that stuff i linked to.
22:29 < venturecommunist> i'm just learning the terminology, i've seen assembly used a lot
22:29 < kanzure> for quickest results you can outsource to scienceexchange.com and then slowly bring parts of the process in-house as you cover more of it
22:30 < venturecommunist> very cool, i think i'll do that
22:31 < venturecommunist> kanzure: you think they would have a problem wth me requesting this, not being a lab?
22:31 < venturecommunist> oh i connect with a lab first? ok
22:31 < kanzure> yes i've heard anecdotes that they are not thrilled about it but fuck 'em
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22:33 < venturecommunist> kanzure: this thing that's called amplified insert cause you amplify the insert plasmid in a PCR, it's highly do-able except for the purification step
22:34 < venturecommunist> what i mean is that i have uncertainty re: purification
22:34 < venturecommunist> it mentions a dna binding column several times as if it's an advantage / low requirement
22:34 < kanzure> purification happens with liquid chromatography columns
22:35 < venturecommunist> the original paper says "dna binding column" not sure if that's the same?
22:35 < kanzure> why don't i just buy the equipment you need.. what are you doing again? i hvaen't looked.
22:36 < venturecommunist> it's a broad spectrum anti-viral therapeutic
22:36 < venturecommunist> based on a fusion protein expressed in a bacterium so i assume e coli
22:36 < venturecommunist> it's effective in vitro on 15 viruses
22:36 < venturecommunist> 2 in vivo mouse models
22:37 < kanzure> some types of proteins can be made in vitro
22:37 < venturecommunist> domain 1 is HIV1 TAT usually, but the concept is to pass the blood brain barrier, get into cells and my own theory is that it would evade HIV1
22:38 < venturecommunist> domain 2 is PKR which binds double stranded RNA and initiates apoptosis via a lengthy process nearly all viruses actively subvert
22:38 < venturecommunist> APAF1 induces apoptosis very immediately, by dimerizing with itself when multiple DRACOs attach to some dsRNA
22:39 < venturecommunist> that causes caspase-9 to dimerize which induces very quick apoptosis
22:40 < venturecommunist> so it's called dsRNA activated caspase oligomerizer
22:40 < venturecommunist> (draco)
22:44 < venturecommunist> kanzure: i don't think you'd want to make free HIV1 TAT in vitro
22:44 < venturecommunist> vivo*
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22:45 < venturecommunist> some theories going around that free TAT could be causal in Alzheimer's
22:45 < venturecommunist> not that there's a human gene for making it?
22:45 < venturecommunist> not sure how this all works
22:45 < venturecommunist> PKR and APAF1 are human
22:51 < kanzure> unrelated,
22:51 < kanzure> target malware http://krebsonsecurity.com/2014/01/a-first-look-at-the-target-intrusion-malware/
22:51 < kanzure> fenn: ted chiang things for you, http://www.nature.com/nature/journal/v436/n7047/full/436150a.html
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23:11 < venturecommunist> kanzure: originally i was thinking "column" meant spin column so i was worried
23:12 < venturecommunist> now i'm thinking maybe it means a kit like this https://www.lifetechnologies.com/us/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/purelink-hipure-plasmid-filter-purification-kits.html
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