--- Log opened Thu Jan 23 00:00:42 2014
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05:45 < kanzure> bleep bloop
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06:53 < JayDugger> bloop bleep (at more than 2 kb/s)
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06:56 < kanzure> yep..
07:06 < pete4242> How's the roadmap looking? Are we making progress?
07:09 < kanzure> i'd say yes, why are you asking and who are you and why are you saying "we"
07:09 < FourFire> pete4242, some things are happening, others aren't as fast ad we'd like
07:10 < FourFire> but yeah, we're getting there
07:10 < kanzure> fuck you FourFire you've never even seen the roadmap how would you know
07:10 < kanzure> none of this makes sense
07:10 < FourFire> kanzure, please don't swear at me withou reason
07:10 < kanzure> it is good reason. i am highly confident that you haven't.
07:10 < FourFire> PM
07:10 < kanzure> i don't see a pm
07:11 < pete4242> I'm nobody (important). I mean 'we' as humanity..
07:11 < kanzure> oh, screw humanity
07:11 < kanzure> you shouldn't care about whether or not "humanity" is making progress
07:11 < pete4242> You don't mind that people are suffering?
07:11 < kanzure> what does that have to do wth anything?
07:11 < kanzure> *with
07:11 < pete4242> It upsets me.
07:12 < pete4242> I think we have a moral duty to help people.
07:12 < FourFire> pete4242, currently life sucks for a lot of people
07:12 < pete4242> no shit
07:12 < FourFire> for a very big number of people it sucks so badly that they can't even sustain it
07:12 < pete4242> What should I care about, Kanz?
07:12 < kanzure> well you're in a transhumanism channel
07:12 < pete4242> indeed
07:13 < pete4242> in the transhuman groove
07:13 < kanzure> so i would say personal individual enhancement
07:13 < kanzure> http://diyhpl.us/wiki/declaration is a fairly accurate portrayal of the shared values in this channel
07:13 < pete4242> I'm just one human. Why not try to enhance everyone?
07:14 < pete4242> then we can be transhumanS not just a transhuman
07:14 < kanzure> uh....
07:14 < pete4242> I wonder if that document has changed since I last looked at it.
07:14 < kanzure> "why not try to enhance everyone?" do you include all lifeforms?
07:14 < kanzure> are you an omega pointist or something
07:15 < kanzure> like, do you believe that all lifeforms everywhere should be changed to have intelligence and then uploaded
07:15 < kanzure> there's a strain of transhumanists that believe this, and i forget what they call themselves
07:15 < kanzure> but i think you are one of them
07:15 < kanzure> based on the available evidence you have presented me
07:15 < pete4242> You told me not to speculate, once
07:16 < pete4242> It's something to do with entropy, blah blah
07:16 < pete4242> I won't bore you with philosophy
07:16 < kanzure> i'm not speculating, you just said "why not try to enhance everyone?" and it seems to be a conviction you were positing earlier
07:18 < pete4242> I don't know. I just like being happy, and I'm more happy when the whole universe is more happy.
07:18 < gradstudentbot> Don't even ask how my research is going.
07:18 < kanzure> are you david pearce?
07:18 < pete4242> hehe
07:18 < kanzure> ?
07:18 < pete4242> No. I enjoy eating animals.
07:18 < pete4242> I'll eat anything dumber than me.
07:18 < kanzure> but you see mto be aware of him
07:19 < kanzure> *seem to
07:19 < pete4242> He's a friend.
07:19 < pete4242> I asked him to be the leader of my new cult.
07:19 < kanzure> so are you asking me to show arguments for why i am more interested in personal human enhancement than global humanity enhancement (perhaps forced etc)?
07:19 < kanzure> your.. new cult.
07:20 < pete4242> that's what I said.
07:20 < pete4242> wanna join? We'll have a cool bio lab. In Portugal.
07:21 < kanzure> tiago rodrigues or vitorino ramos
07:21 < kanzure> can't decide which one to guess
07:21 < pete4242> Are they people or places?
07:22 < pete4242> My name is Peter.
07:22 < kanzure> i thought they would be people in the lab already but i guess not
07:22 < kanzure> such slackers
07:22 < pete4242> There is no lab. yet. The people will come from all over the world.
07:22 < kanzure> well they will probably show up eventually
07:22 < pete4242> Should we welcome them?
07:22 < kanzure> then why would you say portugal
07:23 < kanzure> i would appreciate it if you would say what you mean instead of bullshitting me
07:23 < FourFire> "<pete4242> I'll eat anything dumber than me." yay, do you eat retards?
07:23 < pete4242> Because that's where we plan to build the lab.
07:23 < kanzure> FourFire: cannibalism is a thing that happens you know..
07:23 < pete4242> Only the delicious ones.
07:23 < FourFire> kanzure, duh
07:24 < pete4242> I do try to say what I mean. Any help is appreciated.
07:24 < kanzure> help about what
07:24 < FourFire> so pete4242 what causes life as or more intelligent than yourself to have a right of happiness?
07:25 < kanzure> ugh this is going to be a philosophical dick flinging contest right here
07:25 < pete4242> There are no rights. Just things I want and things I don't want.
07:25 < kanzure> i'd rather you not get into an argument about hedonistic imperatives
07:25 < pete4242> I already told you I won't bore you with philosophy!
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07:26 < FourFire> "dick flinging" gives an interesting mental picture
07:26 < FourFire> it's  a .gif
07:26 < pete4242> I don't want to know.
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07:26 < pete4242> I hate meat.
07:26 < pete4242> unless I'm eating it.
07:27 < FourFire> cold, raw chicken breasts rubbing against your palms give you the willies?
07:27 < pete4242> Nah. Dead meat is fine.
07:27 < pete4242> I hate thinking meat.
07:28 < FourFire> can we more closely define this "hate" ? because hate can mean anything from vaguely annoyed at to "will not stop until I rid the earth of this scourge upon existence"
07:28 < pete4242> heh. Kanz will get mad. So no.
07:29 -!- mode/##hplusroadmap [-o kanzure] by kanzure
07:29 < kanzure> can't we all just write software and build hardware
07:29 < pete4242> we can.
07:29 < kanzure> instead of planning to grey gooify the world
07:29 < kanzure> that will come naturally
07:30 < FourFire> kanzure, I'd just like to state that I agree with parts of but not all of the declaration
07:30 < pete4242> edible hardware is good. No computation to do? well it makes a tasty snack!
07:31 < FourFire> yeah, I'll go on with furthering life extension technology
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08:20 < kanzure> i don't know what you mean
08:20 < kanzure> please be more specific
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10:44 < delinquentme> hue hue hue
10:44 < delinquentme> ZOMGz
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12:35 < Burninate> paperbot: http://rsta.royalsocietypublishing.org/content/371/1992/20120269.full.pdf+html
12:35 < paperbot> http://libgen.org/scimag/get.php?doi=10.1098%2Frsta.2012.0269
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15:36 < delinquentme> paperbot,  http://www.nature.com/nrd/journal/v5/n3/full/nrd1985.html
15:36 < paperbot> http://libgen.org/scimag/get.php?doi=10.1038%2Fnrd1985
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16:17 < delinquentme> 10 times the energy density of the lithium-ion batteries in your mobile devices.
16:17 < delinquentme> sugar-powered-biobattery paperbot  http://www.nature.com/ncomms/2014/140121/ncomms4026/full/ncomms4026.html
16:19 < delinquentme> paperbot  http://www.nature.com/ncomms/2014/140121/ncomms4026/full/ncomms4026.html
16:19 < paperbot> http://libgen.org/scimag/get.php?doi=10.1038%2Fncomms4026
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16:21 < drewbug> Anyone get their DNA sequenced?
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16:56 < superkuh> Just 23andMe.
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17:40 < jrayhawk> is there a sequencing service that does anything more than four codon types
17:40 < jrayhawk> for DNA, that is
17:44 < yash> four codon types?
17:44 -!- yash is now known as yashgaroth
17:45 < yashgaroth> like start, stop, coding, and mystery option?
17:46 < jrayhawk> 5-methylcytosine, 5-hydroxymethylcytosine, etc.
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17:47 < yashgaroth> probably not commercially for consumers, because it's super expensive compared to normal sequencing
17:54 < yashgaroth> also, not very useful since there's a lot of variation between cells, and it's not really interesting outside of hard-to-sample tissues
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18:28 <@ParahSailin> thats bases, not codons
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18:34 < justanotheruser> gradstudentbot:
18:34 < gradstudentbot> Paper submitted.
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19:58 <@heath> did someone ever save this http://web.ornl.gov/sci/besd/bsd/nsd/education/afm_high.shtml
19:58 <@heath> archive.org didn't
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20:12 < drewbug> heath: http://web.archive.org/web/20100325192206/http://www.ornl.gov/sci/besd/bsd/nsd/education/afm_high.shtml
20:26 <@heath> http://www.gizmag.com/lego-arduino-nanometer-resolution-atomic-force-microscope-lego2nano/29238/ http://lego2nano.openwisdomlab.net/ http://coidea.cc/project/2a954a49066468ff/solution/a27f0152587e08c6 http://lego2nano.openwisdomlab.net/team2.html http://lego2nano.openwisdomlab.net/img/450px-2+4_darklaser.jpg
20:26 <@heath> cool, thanks drewbug
20:29 < kanzure> hah,
20:29 < kanzure> "The card fees gasoline retailers pay are staggering. They are on average the second-highest operating cost for gas retailers (higher than rent on their stores). The fees were more than $11 billion last year – 87% higher than the entire industry’s profits. That makes six years in a row that the industry paid more in card fees than it made in profits. Those fees add an average of 7 cents per gallon to the price of gasoline sold in the ...
20:29 < kanzure> ... United States and the fees have been exploding. Between 2004 and 2011 while the price of gasoline went up 80%, card fees increased 180%"
20:29 < kanzure> http://www.forbes.com/sites/beltway/2012/05/09/gas-station-operators-are-crying-over-credit-card-fees/
20:30 < kanzure> and the typical: "Ask any business owner who accepts credit cards about swipe fees and you'll get an earful. Every time a customer pays with a card, up to 4 percent of the sale goes back to the banks and credit card companies. Considering that the average profit margin for most retailers is 1-2 percent, these fees drive up prices and make it harder for small businesses to expand, hire more workers or even stay in business."
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21:03 < Burninate> in China I was laughed at for trying to use my Visa-network check card and spent five minutes trying to explain why there was no pin number
21:04 < kanzure> go on
21:04 < Burninate> In the US, anything that goes over a credit network is loss-limited in the event of theft
21:05 < Burninate> whereas if you have a debit card magstripe and pin number you can empty the entire account
21:05 < Burninate> apparently basic credit card transactions are usually pin-protected in China
21:05 < gradstudentbot> I think my PI hates me.
21:06 < Burninate> I have a check card that can go through either network, credit card or debit card
21:06 < drewbug> But you didn't want to do a debit transaction.
21:06 < Burninate> right, because vulnerable
21:06 < Burninate> and they were at a loss for why I would choose the option that involved no pin number at all, or why sucha n option existed
21:06 < drewbug> Heh.
21:06 < drewbug> Did it eventually work?
21:06 < Burninate> yes
21:07 < Burninate> the card itself never failed me, but the banks do have some pretty restrictive policies on overseas cash advance
21:07 < kanzure> yeah, my bank has this weird text box where it asks you to type in travel plan details
21:07 < Burninate> try to get cash out of an ATM and you might find yourself limited to 500 yuan at a time
21:07 < kanzure> no specific format
21:08 < kanzure> just type in the places, some words about your trip
21:08 < kanzure> and magically none of the transactions will be flagged
21:08 < kanzure> seems suspicious
21:08 < kanzure> i don't think they have a fraud prevention department
21:08 < kanzure> it's just a ruse
21:08 < Burninate> yeah, I did that beforehand
21:09 < Burninate> only time I've ever flagged my card was when I got too frisky on a Steam sale
21:09 < kanzure> heh 500 u[B[Bheh 500 yuan
21:09 < kanzure> irc failure
21:10 < gradstudentbot> Well, I can't really talk about it because I'm trying to get it published in Science or Nature.
21:10 < Burninate> the limits varied, some were 2000, some were unlimited
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21:49 < drewbug> http://www.nbcnews.com/science/cloned-cats-glow-6C10405337
21:49 < drewbug> Would it be possible to do that to a human?
21:49 < drewbug> A currently living human, that is.
21:49 < kanzure> sigh
21:49 < drewbug> Sorry if it's been beaten to death.
21:49 < kanzure> well, the cats have been
21:49 < Lemminkainen> possible? yes
21:49 < Lemminkainen> practical? maybe
21:50 < Lemminkainen> cheap? nope
21:50 < kanzure> human glowth is just a natural extension that will also be beaten to death and it will annoy everyone... someone go get stelarc.
21:50 < kanzure> damn, humanglowth.com is available
21:51 < drewbug> gTLDs are coming soon, maybe we can get human.glowth
21:52 < gradstudentbot> The culture got contaminated.
21:52 < Lemminkainen> run it again, gradstudentbot
21:52 < gradstudentbot> I need to send that abstract.
21:52 < Lemminkainen> no you don't, go make some fucking data
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22:02 < drewbug> Why has nobody glowth'd themselves yet?
22:04 < drewbug> And what makes it expensive to do?
22:05 < Lemminkainen> well what vector would you like to use?
22:05 < drewbug> The best one for the job.
22:05 < Lemminkainen> that's debatable
22:06 < Lemminkainen> but let's assume you want to make your skin glow, so you go with a liposomal delivery medium
22:06 < Lemminkainen> say, Lipofectamine-3000
22:06 < Lemminkainen> it runs about $2500/kit for any appreciable amount, and you'll need many kits
22:06 < Lemminkainen> but on top of that, you're going to need to transfect a lot of genetic material as well
22:07 < drewbug> What genetic material would that be?
22:07 < Lemminkainen> if you want permanent glowth, you'll need to transfect your skin cells and, more importantly, your basement membrane cells with a specially designed CRISPR or TALEN driven by a constantly on promoter
22:08 < Lemminkainen> there's an open-source TALENs method called Golden Gate that you could use to design template, order that in, and then grow it in mass
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22:08 < Lemminkainen> then you'd have to purify it en masse (make sure to check against endotoxin) and get it into the Lipofectamine kits
22:09 < Lemminkainen> and after that you'd get to rub it all over your skin, preferably by taking a gentle salt bath and remaining there for 6 hours or more
22:09 < drewbug> And then you glow forever?
22:10 < Lemminkainen> thing is, Lipofectamine is fairly shit at transfection efficiency, so the first time you do this you might get 20% efficacy
22:10 < Lemminkainen> and a lot of dead skin cells (Lipofectamine-3000 just dropped this week with the headline "NOW LESS TOXIC")
22:10 < Lemminkainen> it's still fairly toxic
22:10 < drewbug> What are the other delivery vectors?
22:10 < Lemminkainen> so you'd get to do repeated administrations of your glowey lipofectamine and hope that it keeps working without causing too much pain
22:10 < Lemminkainen> yay
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22:11 < Lemminkainen> there are also adenoviruses, adeno-associated viruses, and a few other viral vectors
22:11 < Lemminkainen> but they tend to come with some unpleasantly inflammatory side-effects and have a bad habit of evolving and shit
22:11 < drewbug> "inflammatory"?
22:11 < Lemminkainen> inflammatory = your immune system reacts
22:12 < kanzure> there is a lot of history of humans getting compromised immune systems because viruses are very good at their function
22:12 < kanzure> inflammatory side-effects include death
22:12 < Lemminkainen> in extreme cases, yes
22:12 < kanzure> although the lentiviral vectors seem like an okay candidate for not killing you
22:12 < Lemminkainen> but that hasn't stopped the EMA from approving Glybera (an AAV) for market
22:13 < kanzure> which one is EMA?
22:13 < Lemminkainen> European Medical Association
22:13 -!- mode/##hplusroadmap [+o kanzure] by ChanServ
22:13 < Lemminkainen> Glybera treats thalassemia for about $1.1M/treatment
22:14 <@kanzure> damn i can't come up with an EMA joke for /topic
22:14 <@kanzure> federal death administration is staying for now
22:14 < Lemminkainen> I think I like the idea of this channel being sponsored by George Church's beard better than Church hisself
22:15 <@kanzure> well the whole point is that george church sponsors everything
22:15 <@kanzure> he probably doesn't even know his complete list
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22:15 < Lemminkainen> I'd like it even better if Feiyang and Hammond would grow beards as part of being included in Editas
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22:16 <@kanzure> so who's to say he's not thinking fondly of hplusroadmap?
22:16 < Lemminkainen> anyway drewbug there's also a bunch of polymer-DNA complex delivery mechanisms as well, but as the blasted patent landscape of the PEGylation craze shows, it works for shit
22:16 < Lemminkainen> I suppose you could try suspending TALENs directly in DMSO and hoping that that works
22:16 < drewbug> Hmm.
22:17 < Lemminkainen> kanzure I hope he masturbates to hplusroadmap
22:17 <@kanzure> uh, okay?
22:17 < Lemminkainen> because that would be hilarious
22:17 < drewbug> Skin cells and basement membrane cells.
22:17 < drewbug> Could you do others?
22:18 < Lemminkainen> depends on how you want to get to other cells
22:18 < Lemminkainen> luciferase/luciferin transfection of buccal cells would be neat, then you'd have red-glowth saliva
22:19 < gradstudentbot> Oh that's interesting, do you want to write a paper together?
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22:19 < drewbug> I like that.
22:19 < drewbug> Why red?
22:19 < drewbug> You could do others, right?
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22:21 <@kanzure> gradstudentbot: aren't you already coauthoring like 40 other papers?
22:21 < gradstudentbot> That's definitely a Nature paper.
22:21 < drewbug> Lol.
22:21 < Lemminkainen> http://www.promega.de/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/?__utma=1.1542101436.1390544448.1390544448.1390544448.1&__utmb=1.1.10.1390544448&__utmc=1&__utmx=-&__utmz=1.1390544448.1.1.utmcsr=google|utmccn=(organic)|utmcmd=organic|utmctr=(not%20provided)&__utmv=-&__utmk=233469086
22:21 < Lemminkainen> there you go drewbug
22:21 <@kanzure> that is the worst url ever
22:21 <@kanzure> what the fuck promega
22:21 < Lemminkainen> hey, they make molecules, not websites
22:21 < Lemminkainen> (or customer support)
22:21 -!- justanotheruser2 [~justanoth@50.121.14.140] has joined ##hplusroadmap
22:21 <@kanzure> it's like they attempted to do seo but then it backfired when they realized none of their customers use the internet
22:21 < drewbug> kanzure: http://www.promega.de/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/ works just as well
22:22 <@kanzure> yeah because when i'm searching for a promoter, "product guide" is in my query
22:22 <@kanzure> yeesh
22:22 < Lemminkainen> ain't worth your worry
22:23 < drewbug> Lemminkainen: Thank you for the link.
22:23 <@kanzure> it's something that could be done better
22:23 <@kanzure> and also, promega itself makes a killing
22:23 <@kanzure> so leaner entities can probably eat its lunch
22:23 < Lemminkainen> no problem drewbug
22:23 < Lemminkainen> and lately I am such a leaner entity
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22:35 < drewbug> Lemminkainen: Could you do luciferase/luciferin transfection of skin cells?
22:35 < drewbug> And the basement membrane cells.
22:35 < drewbug> Instead of fluorescence.
22:36 < Lemminkainen> that's what I was saying
22:36 < drewbug> Oh.
22:36 < drewbug> Cool.
22:37 < drewbug> Thank you.
22:39 < gradstudentbot> Huh, this western worked on the first try.
22:44 < drewbug> Lemminkainen: Hypothetically, if you succeeded, could you then undo it?
22:50 < Lemminkainen> by doing the same thing with an antisense transfection, yes
23:02 < drewbug> Are they any downsides other than cost and being hated by everyone?
23:02 < drewbug> When using the Lipofectamine method, that is.
23:07 < Lemminkainen> toxicology
23:07 < Lemminkainen> Lipofectamine has a bad habit of killing off the cells you're trying to transfect
23:08 < Lemminkainen> problem is, so does almost everything else: GeneJuice, JetPEI, Invivofectamine
23:08 < drewbug> Chance of death is very low, though?
23:09 < Lemminkainen> leghlaHchu'be'chugh mIn lo'laHbe' taj jej
23:10 < Lemminkainen> "a sharp knife is nothing without a sharp eye"
23:10 < drewbug> I do not understand.
23:10 < Lemminkainen> Klingon proverbs aside, the mortality risk is low
23:10 < Lemminkainen> but morbidity risk is high
23:10 < drewbug> What kind of morbidity?
23:11 < Lemminkainen> skin death
23:12 < drewbug> Would it not grow back?
23:12  * Lemminkainen shrugs
23:12 < Lemminkainen> that depends on how good a job you did at transfecting it
23:14 < Lemminkainen> if you get off-site mutagenesis of the basement membrane there might be some major problems
23:14 < Lemminkainen> so best to try a test patch somewhere first
23:14 < drewbug> What do you mean by that?
23:15 < drewbug> "a test patch"?
23:19 < Lemminkainen> a small area of your skin, somewhere not too noticeable, not too flexible, that you can try the transfection on first before slathering it over all of your skin
23:20 < drewbug> Wait, so, it wouldn't spread?
23:20 < drewbug> Could I do it on just one finger?
23:20 < Lemminkainen> yup
23:21 < Lemminkainen> if you're using a liposomal or polymeric transfection methodology, it won't spread past where you apply it (much)
23:21 < drewbug> And it'd still be permanent?
23:21 < Lemminkainen> if you get to the skin stem cells, yeah
23:22 < drewbug> How does one get to the skin stem cells?
23:22 < Lemminkainen> basement membrane penetration
23:22 < drewbug> Gotcha.
23:23 < gradstudentbot> Why did I go to grad school?
23:23 < gradstudentbot> Yeah, but biobricks don't even work.
23:35 < drewbug> Would it be possible (and, if so, practical) to hook it up to a "trigger"?
23:35 < drewbug> A neurological one, perhaps. Or, muscle use.
23:37 < gradstudentbot> I'll be at the microscope.
23:37 < Lemminkainen> ish
23:37 < Lemminkainen> optogenetic triggers are getting better
23:37 < Lemminkainen> a muscle- or neuro-trigger would be more difficult
23:38 < drewbug> Optogenetic wouldn't lead to a feedback loop?
23:38 < Lemminkainen> depends on the frequency of light
23:38 < Lemminkainen> but then, it's just making light, not insulin, so no problem if there does wind up being a loop
23:39 < drewbug> How long would it last if it were an optogenetic trigger?
23:39 < gradstudentbot> How many papers do you have published?
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23:42 < Lemminkainen> the optogenetic trigger would just be "turn on"
23:42 < Lemminkainen> the genetic programming would be permanent if you do it right
23:42 < drewbug> I mean, how long would it stay turned on?
23:42 < Lemminkainen> you could also build in a "turn off" switch
23:42 < drewbug> Or would you need a turn off
23:42 < drewbug> Oh
23:42 < Lemminkainen> as long as you left it on
23:42 < drewbug> Okay.
23:42 < drewbug> Huh.
23:43 < drewbug> Very, very, very cool.
23:43 < drewbug> Two different light frequencies, one for off and one for on?
23:43 < Lemminkainen> yup
23:43 < Lemminkainen> Ed Boyden's group has done that with neurons
23:43 < drewbug> Would happens if both happen?
23:43 < Lemminkainen> null
23:43 < drewbug> *What
23:44 < drewbug> Interesting.
23:44 < Lemminkainen> should be noted I have a vested interest in this
23:44 < drewbug> Oh?
23:45 < Lemminkainen> I'm gearing up to release some really good transfection reagents this summer
23:45 < drewbug> Expensive?
23:45 < Lemminkainen> looking like it will have to be until they're sufficiently idiotproofed
23:46 < drewbug> Any idea on pricing?
23:46 < Lemminkainen> the math we've done so far for them suggests about $700 for a basic kit
23:47 < drewbug> Awesome!
23:47 < drewbug> What would something like this need?
23:47 < Lemminkainen> 1 kit should be enough to fully transfect 1 mouse
23:47 < drewbug> So, a bunch.
23:47 < Lemminkainen> besides the kit itself you'd need some fancy salts and fancy waters and pipettes to actually do the transfection
23:47 < drewbug> Fancy pipettes?
23:48 < drewbug> Oh
23:48 < drewbug> I guess you didn't say that.
23:48 < drewbug> Pipettes?
23:48 < Lemminkainen> micropipettes
23:48 < Lemminkainen> standard molecular biology tool
23:48 < drewbug> What are they needed for, though?
23:49 < Lemminkainen> very precisely and sterilely transferring tiny amounts of expensive liquids
23:49 < drewbug> What step of the process would that be?
23:49 < Lemminkainen> getting your plasmid on the inside of the transfection-enabling bits
--- Log closed Fri Jan 24 00:00:00 2014