--- Log opened Wed May 21 00:00:11 2014
00:08 <@fenn> markdown bullet points are so ugly
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00:11 <@jrayhawk> Asterisks, or dashes?
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00:20 <@fenn> asterisks. how do you do dashes?
00:21 < jrayhawk> Same way.
00:22 <@fenn> do they render differently?
00:26 < jrayhawk> Nope.
00:26 < jrayhawk> If it's the rendering you hate, you can feel free to throw css at either me or the repository.
00:28 < jrayhawk> https://antportal.com/wiki/ has CSS I was reasonably impressed by.
00:28 <@fenn> gah
00:29 <@fenn> apparently paperbot is dumping its debug into my shell on gnusha :\
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00:29 <@fenn> i hate how it renders two bullet points for a double indent and three bullet points for a tripled indent
00:29 <@fenn> in fact i hate bullet points
00:30 <@fenn> fuck markdown
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=154d7cd2 fenn: init >> http://diyhpl.us/diyhpluswiki/.gitignore
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=801ea910 fenn: scratchings >> http://diyhpl.us/diyhpluswiki/draft
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=035cccb5 fenn: preamble >> http://diyhpl.us/diyhpluswiki/draft
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=02919ed7 fenn: more sorting categories >> http://diyhpl.us/diyhpluswiki/draft
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=b8282366 fenn: more words; increase verbiage! >> http://diyhpl.us/diyhpluswiki/draft
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=eddc7b86 fenn: effectiveness definition >> http://diyhpl.us/diyhpluswiki/draft
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=de833d64 fenn: %s/ \(\w\)/- \1/ and yamlify in general >> http://diyhpl.us/diyhpluswiki/draft
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=e44cc84f fenn: rename >> http://diyhpl.us/diyhpluswiki/goals.yaml
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=9235deb1 fenn: Revert "yank hplusroadmap.draft content" >> http://diyhpl.us/diyhpluswiki/declaration/
00:43 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=6b750cbb fenn: pull in goals.yaml >> http://diyhpl.us/diyhpluswiki/.gitignore
00:45 <@fenn> oh, the magic "git filter-branch --subdirectory-filter"
00:46 <@fenn> some day i'll remember how to do this stuff
00:49 <@fenn> wtf markdown, _this_ is the same as *this*?
00:52 < jrayhawk> http://wiki.freedesktop.org/testrepo/ https://secure.freedesktop.org/cgit/testrepo/tree/index.mdwn maybe this helps clarify the indenting situation?
00:53 < jrayhawk> i am not really sure what you're complaining about where
00:53 <@fenn> not really? what is doing on with 1. test translating to 1. 2. 3. 4.?
00:53 < jrayhawk> that's really the browser's doing
00:54 < jrayhawk> Just how numbered lists work.
00:54 < jrayhawk> er, ordered lists.
00:54 <@fenn> but a) there are 13 different levels of indentation, but it gets transformed into 5 because why? and b) they're all separate levels of indentation
00:55 <@fenn> anyway the bullets are distracting
00:56 < jrayhawk> Markdown has heuristics for what ranges of textual indentation apply to what sort of HTML sublisting.
00:56 < jrayhawk> That page was to partially clarify the simple cases for me.
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00:57 <@fenn> why doesnt ----
00:57 <@fenn> render as a horizontal rule?
00:57 < jrayhawk> Hmm?
00:57 < jrayhawk> says
right there
00:57 <@fenn> oh it is invisible here nm
00:58 < jrayhawk> oh, maybe I fucked up that bit of the CSS.
00:58 < jrayhawk> Too lazy to look. Fuck CSS.
00:58 <@fenn> CSS beats markdown
00:58 <@fenn> at least i can override CSS if i want
00:58 < jrayhawk> You know you can override markdown, right?
00:58 <@fenn> er, no?
00:59 <@fenn> there's the [!format] directive which i haven't looked at yet
00:59 < jrayhawk> Support for HTML mixing is a required part of the markdown specification.
01:00 < jrayhawk> Vitriolic attacks on how difficult it is to use are rather ironic when it is readily apparent you have never read the documentation for it.
01:01 <@fenn> i feel justified since it's supposed to be "intuitive" and "easy to use"
01:01 < jrayhawk> Is it?
01:01 <@fenn> "Markdown is intended to be as easy-to-read and easy-to-write as is feasible."
01:02 < jrayhawk> "intuitive"
01:02 < jrayhawk> is more what I am not finding.
01:02 < jrayhawk> "easy" as a lot more potential connotations.
01:03 <@fenn> there are so many wiki syntaxes and things like asciidoc and simpledoc that i've had to learn over the years and they all vary by tiny amounts in ways that are mutually incompatible some of the time
01:04 <@fenn> see i cant even remember what they're called
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01:09 < jrayhawk> s/ as a lot/ has a lot/
01:14 < jrayhawk> also if you're associating markdown with the concept of "wiki syntax" you're projecting motivations the authors never intended again
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01:18 <@fenn> it's the same thing
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01:18 <@fenn> they never would have used [] for links if it weren't descended from wiki syntax
01:18 < jrayhawk> https://secure.piny.be/cgit/piny-hosting/tree/index.mdwn this is the primary intended link format.
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01:22 < jrayhawk> If I were a better person, I would be doing 72 or 80 column linebreaking on those paragraphs.
01:26 < jrayhawk> Reversed Mediawiki links do not make for smoothly readable READMEs.
01:29 <@fenn> The bikeshed color should be ...
01:29 <@fenn> ...blue of course
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01:58 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=5c7ecb26 fenn: test inline declaration syntax >> http://diyhpl.us/diyhpluswiki/declaration/
01:58 < paperbot> ConnectionError: [Errno 111] Connection refused (file "/usr/local/lib/python2.7/dist-packages/requests/models.py", line 625, in send)
01:58 <@fenn> uh oh i broke everything
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03:22 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=efd6e249 fenn: index was included in the bullet point list, attempt to fix >> http://diyhpl.us/diyhpluswiki/index/
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06:00 < chris_99> anyone done sequence mining out of interest
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06:38 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=da1b6677 fenn: fix broken include statement >> http://diyhpl.us/diyhpluswiki/declaration/
06:45 < eudoxia> i should move the cryopatients list from the transhumani wiki to a YAML file
06:49 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=b7e7f33d fenn: test format directive >> http://diyhpl.us/diyhpluswiki/goals.yaml
06:49 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8f9dda50 fenn: Merge branch 'master' of /srv/git/diyhpluswiki >> http://diyhpl.us/diyhpluswiki/
06:50 <@fenn> weird it deleted the link
06:52 <@fenn> ikiwiki doesn't seem to like nesting directives inside other directives
06:52 <@fenn> maybe i should be using templates instead?
06:53 <@fenn> it seems like you should be able to inline a file (transclude it) but also get code highlighting
06:54 < gradstudentbot> Where's my pellets?
06:54 <@fenn> and then "toggle" collapse/expand it
06:54 <@fenn> you can do all these things separately but not together
06:57 < gradstudentbot> Did you do that pset?
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07:04 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=6981b70d fenn: try toggle >> http://diyhpl.us/diyhpluswiki/declaration/
07:04 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=5c2a2a92 fenn: This reverts commit 6981b70d8e39a1a406f95d3cd9aff911535b3869 >> http://diyhpl.us/diyhpluswiki/declaration/
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07:12 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=74ca22ff fenn: eh good enough >> http://diyhpl.us/diyhpluswiki/declaration/
07:12 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=4c8b9204 fenn: remove non functioning directive code >> http://diyhpl.us/diyhpluswiki/goals.yaml
07:13 <@fenn> what the bloody fuck
07:15 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8a7ed8fe fenn: attempt to appease our bullet-point gods >> http://diyhpl.us/diyhpluswiki/goals.yaml
07:16 <@fenn> huh adding whitespace after the list worked
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08:28 < kanzure> what was wrong with bullets?
08:28 < kanzure> or the way the file was
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09:38 < nmz787_i> in case my post is censored (because who knows).... re diybio blogs.... A bunch of those sites haven't published a new post in more than 6
09:38 < nmz787_i> months... what is your limit for considering a blog active?
09:38 < nmz787_i> (amplino, openpcr)
09:38 < nmz787_i> How about non-self-published blog noise (most posts on glowingplant
09:38 < nmz787_i> aren't actually about their own project)?
09:38 <@fenn> when you expect flutes it's whistles
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09:57 < kanzure> i dunno if it matters if they are active or not, the internet does not discriminate the age of your content
09:59 < kanzure> it's unfortunate that mac has hired a blogger for diybio.org
09:59 < kanzure> why not hire someone to, i dunno, build things
09:59 < kanzure> instead of reading rss feeds and an inbox
10:00 < kanzure> it's not like labor is dramatically more expensive than rss labor
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10:28 < chris_99> am i right/wrong in thinking scientists 100 yrs or so ago, had a broader range of interests than those now
10:28 < chris_99> *scientific interests
10:28 < ParahSailin> probably right
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10:29 < FourFire> chris_99, there's much more specialization, sure
10:29 < FourFire> There's more things to learn about a given field and more fields, period
10:30 < chris_99> i quite like the idea of having a broader range of knowledge, as you could maybe find connections between different areas, that you wouldn't if you specialized very deply
10:30 < chris_99> *deeply
10:31 < kanzure> having a broader range of knowledge these days is harder to demonstrate because it sounds like you're just regurgitating content
10:32 < chris_99> hmm, not if you actually practice in those areas too though
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10:43 < chris_99> i would like to become a gentleman scientist ;) if i could just win the lottery...
10:43 < kanzure> why would you need to win the lottery?
10:44 < chris_99> just for the funding, then one could do it full-time, without needing to worry about subsidising it
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10:44 < kanzure> there are other ways of accumulating piles of money besides lotteries.. entirely more efficient methods too.
10:45 < eudoxia> yeah like kanzure's script that mines new cryptocoins
10:46 < chris_99> hmm i wonder if you can apply to funding bodies like the EPRSC without belonging to a uni
10:48 < kanzure> eudoxia, all my other income comes from software consulting and software contracting
10:49 < eudoxia> kanzure: i noticed there isn't a link to heybryan.org/resume.pdf on your page, is that intentional?
10:50 < kanzure> it's because of this epic struggle between (1) the fact that i am bombarded with more offers than i can deal with and (2) my interest in being paid large amounts of money and (3) how much i dislike working
10:50 < kanzure> i'm afraid that if i actually put effort into marketing my work, i might have to do even more work
10:51 < kanzure> and who the fuck wants that?
10:51 < eudoxia> i assumed (1)
10:51 < juri_> :)
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10:54 < kanzure> world's pretty crazy out there at the moment
10:54 < kanzure> degree holders with 4 years of education and 0 years of experience getting $100k offers.. so what do you think people with 3, 5, 10 years of experience can nail? etc
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11:46 < heath> contacts in phoenix?
11:46 < heath> does anyone have contacts in phoenix?*
11:47 < heath> it's strange when you're talking to a higher up in government
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11:47 < heath> ~2 contacts away from the president of the US
11:48 < heath> well..1
11:48 < heath> pres -> advisor -> person i talk with
11:48 < kanzure> heatsync is in phoenix i think?
11:48 < heath> right
11:48 * heath is idling in the room
11:48 < kanzure> their resident transhumanist is Jacob Rosenthal
11:48 < heath> not as much activity as the hackerspace in hsv (huntsville) or even bna (nashville)
11:49 < kanzure> why do you only have villages
11:50 < gradstudentbot> Who the hell stole my pipette?
11:57 < Mokstar> yay, my 23andme results are back
11:57 < chris_99> do they give you raw DNA data now, i forget
11:57 < Mokstar> oh neat, I'm related to jimmy buffet
12:00 < Mokstar> chris_99, yes, but no health analysis
12:01 < chris_99> how much does that cost ye
12:01 < Mokstar> ooh, I've got 3.1% Neanderthal
12:01 < Mokstar> 23andme doesn't sell that anymore
12:01 < Mokstar> I'll have to get it done on my own
12:03 < chris_99> i think i'd rather not know if i've got disease X though
12:03 < Mokstar> what's a good service to analyze this data?
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12:19 < kanzure> just run it against snpedia
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13:11 < kanzure> opencascade has a custom garbage collector?
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13:21 < jrayhawk> chris_99: genetic diseases aren't usually deterministic
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14:00 < kanzure> .title http://mikejmoffitt.com/wp/?p=270
14:00 < yoleaux> Michael J Moffitt's Project Log » Blog Archive » NeoVGA: Neo Geo Line Doubler in VHDL
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15:43 < kanzure_> iiiiiiiii
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15:44 < eudoxia> i sure hope that wasn't some kind of irssi exploit
15:45 < kanzure_> just ssh lag
15:46 < kanzure_> it's impressive that irssi is okay with a lag of 159
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15:49 < kanzure_> huh
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15:50 < kanzure_> Join to ##hplusroadmap was synced in 422 secs
15:50 < kanzure_> there we go
15:51 < justanotheruser> Time for another freenode DoS?
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18:30 < kanzure_> presidential bioethics committee complaining about neuroscience: http://www.bioethics.gov/sites/default/files/Gray%20Matters%20Vol%201.pdf
18:32 < kanzure_> "However, beyond the distinction between treatment and enhancement, cognitive enhancement raises pressing ethical issues concerning equitable access to enhancements and their benefits, appropriate management of risks, and obligations and freedoms to enhance or not.26"
18:33 < kanzure_> "recommendation 1: stop paying us and allow the committee to disband"
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18:36 < catern> if only
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19:35 < QuantumG> so, have ya read beggars in Spain?
19:38 < kanzure_> no
19:38 < QuantumG> it's not bad
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19:49 < kanzure_> if you wanted an efficient government system of granting time-limited commerce-limited monopolies, why should it have anything to do with "technology disclosure"?
19:50 < QuantumG> you shouldn't :)
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19:51 < kanzure_> i'm just contemplating ideas about abolishing the patent system; really i seem to care more about the patent system leaving technology alone more than anything
19:51 < kanzure_> you will not be able to convince everyone to give up their patents because "you will be destroying trillions of dollars"
19:52 < kanzure_> but you could possibly convince them to get rid of the technology component to patents
19:52 < kanzure_> (disclosure of technical machines can happen some other way, like through the public domain or something)
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19:52 < QuantumG> so, do you agree with me that trade secrets are better than patents?
19:52 < QuantumG> (because at least trade secrets can be busted by interested reverse engineers)
19:52 < kanzure_> i'm not sure, what are the trade secrets keeping secret?
19:53 < QuantumG> how to make stuff, probably
19:53 < kanzure_> i don't think that trade secrets are better or worse than patents for disclosing technology
19:53 < kanzure_> at least with a trade secret you can choose whatever representation of the data you want
19:53 < kanzure_> so it's probably better
19:55 < kanzure_> (rather than "serialize to legalese" as the de facto mandatorial standardo)
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19:58 < kanzure_> and if patents lose their technology component, you could possibly shift them to be more about dividing up legal protection regarding commerce revenue or something for different industries... it sounds preposterous, but it's just a more efficient way of saying what the current system is trying to say anyway.
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20:03 < kanzure_> not sure how to make that seem less ominous. the current situation has a bunch of advantage by way of obfuscation and indirection.
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20:07 < QuantumG> then you'll get secrecy and protectionism
20:07 < QuantumG> current situation: I won't bother reverse engineering this because I could just go read the patent, and even when I know how, the government will stop me from using that knowledge
20:07 < QuantumG> your situation: I won't bother reverse engineering this because even if I succeed the government will stop me from using that knowledge.
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20:07 < QuantumG> my situation: the government protects no-one, there's a lot more secrecy, but also a lot more reverse engineering.
20:07 < kanzure_> no, in "your situation", the government would not stop you from using the knowledge
20:08 < QuantumG> apparently this is bad because only big companies can do reverse engineering.. little companies need to be protected. I've never seen any proof of this assertion.
20:08 < kanzure_> (or could you explain how/why it would? remember, in "your situation", patents have lost their technology component)
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20:08 < QuantumG> yeah, so instead they're carving out markets
20:09 < QuantumG> right?
20:09 < kanzure_> yes, probably some additional tax revenue or some shit, i have no idea
20:09 < kanzure_> "participatory patent tax"
20:09 < QuantumG> Microsoft gets the software market (or some bit of it), so what's the point of trying to make a better word processor now?
20:09 < kanzure_> rate-adjusted by industry or some crap haha
20:10 < kanzure_> that "the point" was never the point
20:11 < kanzure_> there was a fairly fascinating empirical study that shows that patents actually reduce innovation
20:11 < kanzure_> the study was done by someone who was pro-patents, and then he looked at his data and frowned
20:11 < kanzure_> *innovation and related incentives
20:11 < QuantumG> compared to what?
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20:11 < kanzure_> http://archive.mises.org/18812/andrew-torrance-patents-and-the-regress-of-useful-arts/
20:12 < QuantumG> I remember reading this a while ago.. but I'm not reading it again, so help me out
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20:12 < kanzure_> yeah i don't remember that detail, sorry
20:13 < kanzure_> at the moment if you have a word processor patent, you can reasonably get it in front of a judge and extract some revenue
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20:14 < kanzure_> (patent licensing revenue is the name of this particular game)
20:20 < QuantumG> .. and?
20:20 < kanzure_> huh? i was responding to your microsoft point
20:21 < kanzure_> heh what if it was a guessing game, where you had to come up with some n-digit number, and the closest guesses get the patents/markets awarded to them, based on some distance or something
20:21 < kanzure_> and then it's some weirdo "monopoly lottery"
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20:26 < kanzure_> hrm i suppose that's more random than "have a bunch of wizards show up in court and say things about magic that nobody understands"
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20:28 < QuantumG> that's gunna be around no matter what you do
20:28 < QuantumG> if there's no patent system, the court cases will be about whether or not Bob violated his employment contract when he left to go work for a competitor
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20:29 < kanzure_> yeah but if the courts are just going to end up in a wishy-washy interpretation battle (like the same one that happens over the constitution), you might as well have the USPTO settle the argument ahead of time by doing its job (granting monopolies, definitively or something)
20:29 < QuantumG> see, for example, John Carmack and the Occulus Rift.
20:29 < kanzure_> if you're not going to write down what you're actually granting the patenteeor, then you're really just deferring it to the court case, and for sufficiently magical things, it's more or less random either way too
20:29 < kanzure_> because often legalese is either too specific or overly general
20:29 < kanzure_> and you can't figure out what parts the uspto was really intending to grant
20:30 < kanzure_> QuantumG: is there a court case about that particular one?
20:30 < QuantumG> http://popcultureblog.dallasnews.com/2014/05/zenimax-and-id-software-have-filed-a-lawsuit-against-oculus-vr-and-dallas-based-john-carmack-is-in-the-middle.html/
20:30 < QuantumG> .title
20:30 < yoleaux> Zenimax and Id Software have filed a lawsuit against Oculus VR, and Dallas-based John Carmack is in the middle
20:31 < kanzure_> i knew there was something problematic about his job switch, didn't know it was a lawsuit yet
20:32 < QuantumG> almost every announcement of a company with deep pockets acquiring a smaller outfit is accompanied by them.
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21:15 < QuantumG> http://quantumg.net/aprt-short.gif because animation
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21:16 < kanzure_> QuantumG: can you render that using electrostatic potential maps instead? kthx
21:17 < kanzure_> and if transparency is an option then enable that
21:17 < kanzure_> mayavi2 might have this
21:17 < QuantumG> doing that now (no transparency)
21:18 < kanzure_> it's pre-computed, so rendering is fast, right?
21:18 < QuantumG> it's reasonably fast, yeah
21:18 < QuantumG> ~15 sec per frame
21:22 < QuantumG> http://quantumg.net/aprt-short-surface.gif
21:22 < kanzure_> hrm
21:22 < kanzure_> cool
21:23 < QuantumG> purple = PRPP binding site, blue = adenine binding site .. this is 7 different homologs
21:23 < QuantumG> I have two more, but I dropped them cause they're long form, not short form, so they look too out of place
21:25 < QuantumG> http://quantumg.net/both.html
21:26 < kanzure_> do you have any good papers on rational protein design?
21:28 < QuantumG> Beyond directed evolution—semi-rational protein engineering and design, Directed evolution made easy, Microfluidic Compartmentalized Directed Evolution, Protein Design by Directed Evolution, Recent trends in biocatalysis engineering
21:29 < kanzure_> yes microfluidic compartmentalized emulsion stuff is fun
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21:29 < kanzure_> but iirc there are some people that know how to get specific holes and arms on proteins these days?
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21:30 < QuantumG> so that first paper tells me
21:31 < kanzure_> paperbot: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982887/pdf/nihms235064.pdf
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21:31 < kanzure_> paperbot: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982887/pdf/nihms235064.pdf
21:31 < paperbot> ConnectionError: [Errno 111] Connection refused (file "/usr/local/lib/python2.7/dist-packages/requests/models.py", line 625, in send)
21:31 < kanzure_> o_____o
21:32 < kanzure_> paperbot: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982887/pdf/nihms235064.pdf
21:32 < paperbot> TypeError: unicode() argument 2 must be string, not None (file "/usr/local/lib/python2.7/dist-packages/requests/models.py", line 825, in text)
21:32 < kanzure_> back to the drawing board..
21:32 < kanzure_> did people used to have drawing boards to go to?
21:33 < QuantumG> yeah, gimme 3 minutes
21:33 < kanzure_> nah, ncbi has it
21:33 < kanzure_> i just wanted paperbot to archive it
21:33 < kanzure_> it is usually capable of archiving a public access pdf
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21:36 < QuantumG> http://quantumg.net/both.html added links
21:39 < kanzure_> in the ncbi-hosted pdf, there's a reference to a study where tunnels in proteins were improved by a handful of different amino acid residues
21:40 < kanzure_> prolly applies to every protein-with-a-hole in that protein family
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21:46 < QuantumG> "Directed evolution made easy" is about PACE. You've heard of that?
21:46 < kanzure_> yes
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21:47 < kanzure_> wait.. acronym overload.
21:47 < kanzure_> is it this one? http://diyhpl.us/~bryan/papers2/bio/A%20system%20for%20the%20continuous%20directed%20evolution%20of%20biomolecules.pdf
21:47 < QuantumG> yup
21:48 < kanzure_> the t7 rna pol one
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21:49 < kanzure_> i don't remember the details of this method. did they have a library of different phages?
21:51 < kanzure_> aha, accessory plasmid and mutagenesis plasmid
21:52 < QuantumG> "First, a crucial gene for phage replication is removed from the phage’s genome and placed in an ‘accessory plasmid’ in E. coli. Expression of this gene can then be tied to a wide range of biological activities,"
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21:54 < QuantumG> "Engineered bacteria and phage are then placed in vessels called lagoons, such that a continuous stream of E. coli move in and out of the vessel. The E. coli only stay in the lagoon for about 20 or 30 minutes, just long enough for phage infection and replication to occur. Only phage that activate
21:54 < QuantumG> the essential gene in the E. coli plasmid can reproduce and infect incoming bacteria. The better the phage is at inducing gene expression in the accessory plasmid, the more phage are produced."
21:57 < QuantumG> .. and, ya know, it's all glassware.
21:57 < kanzure_> their transfection method doesn't seem to require calcium chloride what's going on
21:57 < kanzure_> is that legal
21:58 < QuantumG> (if that's a joke, I missed it)
21:58 < kanzure_> "by heat shock"
21:58 < kanzure_> "NEB Turbo competent cells"
21:59 < kanzure_> oh, electroporation too
21:59 < kanzure_> electroporation sort of counts as glassware
22:00 < sheena> someone is putting new DNA markers in Ecoli?
22:00 < sheena> i heard it on the radiooo
22:00 < nmz787> hi didly ho
22:00 < kanzure_> nucleotides
22:00 < nmz787> nuculartides
22:00 < nmz787> its pronouced nucular
22:01 < gradstudentbot> I am busy researching.
22:04 < Mokstarboush> New cule-air, Ms. Aisles.
22:04 < kanzure_> .ety nuclear
22:04 < yoleaux> nuclear (adj.): "1846, "of or like the nucleus of a cell," from nucleus + -ar, probably by influence of French nucléaire. Use in atomic physics is from 1914; of weapons, from 1945. Hence nuclear physics (1933), nuclear energy (1941), nuclear war (1954)." — http://etymonline.com/index.php?term=nuclear
22:04 < kanzure_> .ety nucléaire
22:04 < yoleaux> Sorry, I couldn't find the etymology of that.
22:05 < Mokstarboush> .ety jazz hands
22:05 < yoleaux> Sorry, I couldn't find the etymology of that.
22:05 < kanzure_> .ety nucleus
22:05 < yoleaux> nucleus (n.): "1704, "kernel of a nut," 1708, "head of a comet," from Latin nucleus "kernel," from nucula "little nut," diminutive of nux (genitive nucis) "nut," from PIE *kneu- "nut" (cognates: Middle Irish cnu, Welsh cneuen, Middle Breton knoen "nut," …" — http://etymonline.com/index.php?term=nucleus
22:05 < kanzure_> "little nut" :)
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22:09 < Mokstarboush> yay! I don't have MTHFR mutations! http://imgur.com/NAfQlFe
22:09 < kanzure_> .title http://www.bbc.com/news/blogs-magazine-monitor-23178484
22:09 < yoleaux> Egypt crisis: Why are Cairo protesters using laser pens?
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--- Log closed Thu May 22 00:00:12 2014