--- Log opened Thu Jun 19 00:00:03 2014
--- Day changed Thu Jun 19 2014
00:00 < kanzure> oh, the others are columns
00:00 < nmz787> so that would be 11
00:00 < gene_hacker> well my idea of how to do it is with a bunch of syringes, and a robot that indexes between them
00:00 < kanzure> nmz787: look at page 219
00:00 < kanzure> gene_hacker: you could do a rotary plate with syringes pointing down
00:00 < kanzure> vertical syringes i mean
00:01 < gene_hacker> we'll see
00:01 < nmz787> ParahSailin: I'll send the waste to you then
00:01 < nmz787> :D
00:01 < gene_hacker> but if I make a synthesizer, I'd really like to make one of those picochip ones that uses photogenerated acid
00:02  * nmz787 looking
00:02 < gene_hacker> http://gaolab.chem.uh.edu/graphics/figure6.gif
00:03 < kanzure> gene_hacker: yep, i shipped a pile of parts to nmz787 for dmd photolithography
00:03 < nmz787> but doesn't that trade-off for addressibility later?
00:03 < gene_hacker> addressability?
00:03 < kanzure> iirc there's a separate photochemical reaction for binding/unbinding beads that you could use
00:03 < nmz787> the point is to not have valving on-chip, right?
00:03 < kanzure> yes
00:03 < gene_hacker> yes, you address with light
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00:04 < nmz787> kanzure: idk about the binding/unbinding
00:04 < nmz787> i thought that would be a global operation
00:04 < nmz787> i'm familiar with this idea for making microarrays
00:04 < kanzure> george church minions did micropipette pick-and-place of beads on a microarray in open air
00:04 < gene_hacker> http://gaolab.chem.uh.edu/res3.htm
00:05 < nmz787> that you then remove from the column all at once and do gibson assembly on or whatever
00:05 < gene_hacker> of course, one can also use a regular DNA synthesizer to drive the picoarray...
00:05 < delinquentme> nmz787, https://www.facebook.com/nanoopticdevices
00:07 < nmz787> delinquentme: cool
00:07 < gene_hacker> awesome, I can't wait until phones come with those built in
00:07 < kanzure> you could do a microarray/picoarray without bonding to another surface (just use pressure), remove pressure/clamps when you're done, then retrieve beads with micropipette at specific locations to get specific sequences
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00:08 < gene_hacker> the point of a picoarray isn't to get a specific sequence
00:08 < gene_hacker> it's to generate a bunch of strands that you can combine into a big one
00:08 < kanzure> if you have a 1024x768 array you might as well synthesize more than one sequence
00:09 < gene_hacker> why?
00:09 < nmz787> right but then you have to do bulk-reactions, you can't do purification on each nano-reactor in the picoarray
00:09 < kanzure> because if you are synthesizing the same sequence 1 million times over, you could just use a traditional column without the spatial light modulator
00:09 < gene_hacker> you will still have to combine some of them
00:09 < nmz787> but avoiding bulk-reactions is much more interesting to me :P
00:10 < kanzure> yes you'll always have to combine basically
00:11 < gene_hacker> and if you can get the combination step to work
00:11 < gene_hacker> you can make really long stuff
00:11 < nmz787> and i'm interested in doing liquid-phase chemistry, using under/near-stoichiometric ratios of reagents
00:12 < gene_hacker> stuff so long it's dangerous
00:12 < nmz787> combination is fine, I just want to be sure of what I have before I add it to something else
00:12 < nmz787> I didn't mention length
00:12 < nmz787> ah, but I guess I see your point
00:13 < nmz787> delinquentme: "Now you can get a Nano-Stick spectrometer for only $2,200!"
00:13 < nmz787> delinquentme: 760nm-920nm
00:13 < nmz787> psh
00:13 < nmz787> maybe for doing fiber taps
00:13 < kanzure> 760-920nm sounds inconvenient
00:13 < kanzure> what about all the other spectrum
00:14 < kanzure> only doing gang color approved spectrum eh?
00:14 < gene_hacker> I don't think that's even gang color approved
00:14 < kanzure> damn
00:14 < nmz787> paperbot: http://www.nature.com/nature/journal/v502/n7471/full/nature12607.html
00:15 < kanzure> .title
00:15 < yoleaux> Coherent Raman spectro-imaging with laser frequency combs
00:15 < nmz787> paperbot: http://dx.doi.org/10.1038/nature12607
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00:15 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/b56e00056b48a1fbf7d6e05b6b92d756.txt
00:15 < paperbot> http://libgen.org/scimag/get.php?doi=10.1038%2Fnature12607
00:16 < nmz787> http://diyhpl.us/~nmz787/pdf/Coherent_Raman_spectro-imaging_with_laser_frequency_combs.pdf
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00:17 < nmz787> "Resolution 0.15 nm Spectral range 500-1100 nm"
00:17 < nmz787> http://www.nanoopticdevices.com/#!products/cee5
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00:24 -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | sponsored by george church and the NRA, banned by the Federal Death Administration (4 times) | this channel is LOGGED: http://gnusha.org/logs | http://diyhpl.us/wiki | not intentionally unrepeatable
00:24 -!- Topic set by kanzure [~kanzure@131.252.130.248] [Fri Jun  6 17:48:33 2014]
00:24 [Users ##hplusroadmap]
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00:24 !morgan.freenode.net [freenode-info] why register and identify? your IRC nick is how people know you. http://freenode.net/faq.shtml#nicksetup
00:24 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010
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00:27 < kanzure> what
00:28 < kanzure> oh there it is. it logged back in.
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02:08 < archels> paperbot: http://iopscience.iop.org/0957-0233/25/1/015501/pdf/0957-0233_25_1_015501.pdf
02:08 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/b1334cb8b50bfb771b7986074871256b.pdf
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03:35 < night> Sobering approximation of the day:
03:36 < night> with average luck, you get 1000 weeks of childhood, 2000 weeks of adulthood, and 1000 weeks of being old
03:36 < night> that is not many weeks
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06:04 < justanotheruser> Anyone here etch their own PCBs?
06:09 < chris_99> i have done a while ago
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06:24 < justanotheruser> chris_99: what is the best place to buy  pre-coated circuit boards
06:25 < chris_99> you mean photosensitive boards?
06:25 < justanotheruser> ya
06:26 < chris_99> ah i'm in the UK, so i'd probably buy from Farnell, but Digikey or anyone should sell them
06:26 < justanotheruser> ok, I'll check out digikeys selection. Gtg, thanks
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09:17 < ebowden> paperbot: http://www.sciencemag.org/content/333/6047/1307
09:17 < paperbot> http://libgen.org/scimag/get.php?doi=10.1126%2Fscience.1205527
09:18 < ebowden> paperbot: http://www.sciencemag.org/content/333/6047/1307.full
09:18 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/Multi-Input%20RNAi-Based%20Logic%20Circuit%20for%20Identification%20of%20Specific%20Cancer%20Cells.pdf
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09:48 < nmz787_i> justanotheruser: i etched my own PCB using copper-clad, black spray paint, and a CO2 laser cutter
09:49 < nmz787_i> justanotheruser: ebay has plenty of pre-coated boards though, for pretty cheap
09:49 < chris_99> is the quality the same as UV boards doing it that way though
09:53 < justanotheruser> nmz787_i: any sellers you reccomend?
09:54 < nmz787_i> chris_99: are you asking is the quality as good? idk never did the pre-filmed method!
09:54 < chris_99> yes i am
09:54 < nmz787_i> justanotheruser: nah, cheap and decent shipping time is what i usually check
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09:54 < nmz787_i> justanotheruser: as long as the reviews aren't horrible too
09:55 < nmz787_i> chris_99: I was able to make a pad for an SSOP chip
09:55 < justanotheruser> nmz787_i: is there a more common phrase for them?
09:55 < justanotheruser> 0 results found for pre-coated circuit board
10:01 < archels> search for photoresist
10:01 < archels> also, consider that you can get PCBs from places like seeedstudio for *really cheap*
10:02 < justanotheruser> archels: yeah, and really slow right?
10:02 < chris_99> yeah, esp. as they do > 2 layer, fancy silk screen etc.
10:02 < chris_99> not really
10:02 < chris_99> i got one from china within like a week
10:02 < chris_99> i payed extra of course though
10:02 < justanotheruser> oh
10:02 < justanotheruser> what is it? Like $1/per?
10:03 < archels> seeed is plenty fast, if you skimp out on shipping it'll take 2 weeks longer
10:04 < archels> let's see, I just paid $60 for five 10x20 cm double layer
10:04 < chris_99> i paid 64 USD for 10x 2 layer, black boards, for fast shipping
10:04 < chris_99> from Hackvana
10:05 < archels> there's also oshpark.com
10:06 < nmz787_i> justanotheruser: keyword should be dry-film or film pcb or photosensitive or photoresist
10:06 < justanotheruser> nmz787_i: This is a good deal right? http://www.ebay.com/itm/20x-Negative-Dry-Film-Photoresist-6x8-A5-for-PCB-Prototype-DIY-Developer-Inst-/251467232236?pt=LH_DefaultDomain_0&hash=item3a8c9d73ec
10:06 < archels> oshpark manufactures their boards in the US
10:07 < nmz787_i> i use OSHPark... I tried seeed but I ordered from OSHPark and seeed the same night and by the time seeed was almost ready to send my boards I'd already received mine from OSHPark so I cancelled my seeed order
10:07 < nmz787_i> justanotheruser: seems decent enough
10:07 < justanotheruser> nmz787_i: do you usually cut your boards?
10:08 < nmz787_i> I actually don't know if they get their boards from the U.S.... but they certainly break up the boards and re-mail them from the U.S.
10:08 < nmz787_i> justanotheruser: I've cut a board once, a better tool is a nibbler (i think its called)
10:08 < gradstudentbot> Where are the pipettes?
10:08 < chris_99> gradstudentbot, back to work boy!
10:08 < gradstudentbot> We simply don't do enough titrations in my lab.
10:09 < nmz787_i> justanotheruser: like this http://www.amazon.com/Parts-Express-Nickel-Plated-Nibbling/dp/B0002KRACO/
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10:13 < chris_99> also another advantage of getting the boards made for you, they can do vias
10:23 < nmz787_i> oshpark is $1.66/sq inch for 2-layer and twice that for 4-layer
10:24 < kanzure> i wonder if electroosmotic flow could be used to cool a consumer-grade cpu
10:25 < kanzure> why am i still using a fan to cool my cpu
10:26 < chris_99> exactly, when we could be using magnetocaloric refridgeration
10:26 < kanzure> .wik magnetic refrigeration
10:27 < yoleaux> "Magnetic refrigeration is a cooling technology based on the magnetocaloric effect. This technique can be used to attain extremely low temperatures, as well as the ranges used in common refrigerators." — http://en.wikipedia.org/wiki/Magnetic_refrigeration
10:28 < nmz787_i> kanzure: we are looking into microfluidics in general, I don't know the details
10:28 < kanzure> huh?
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10:29 < nmz787_i> i doubt it would be electroosmotic though, it would probably just be heat pipes (where the heat induces a thermal current in the contained fluid)
10:29 < nmz787_i> (intel)
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10:33 < kanzure> justanotheruser: haha https://github.com/piggycoin/newpiggycoin/commit/c68aa2304b175d3221539c6bda138ab9d4128d2f (they ran chmod 755 on everything for no good reason)
10:34 < justanotheruser> kanzure: what is the context of this?
10:34 < kanzure> nvm, that is boring
10:35 < justanotheruser> new scamcoin?
10:35 < kanzure> oh probably
10:35 < kanzure> dunno
10:35 < kanzure> 10:16 < Vitallium> detronizator: I pushed latest changes to github. We should able to build PhantomJS 2 for all platforms now
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10:55 < kanzure> 10:48 < sgraber_> You understand a 'good' uv formulation chemist makes +$70/hr at valspar or ppg  and r&d time is normally $100/hr
10:55 < kanzure> not bad
10:55 < kanzure> cheap compared to programmers, lawyers, etc
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11:17 < nmz787_i> kanzure: what channel was that said in?
11:18 < nmz787_i> kanzure: if you could hire me a chemist... that would be insanely helpful
11:18 < kanzure> shane graber is one possible chemist, but another one is david treadwell
11:18 < nmz787_i> I might need to quit my day job for that
11:18 < kanzure> (not the david treadwell you see on diybio)
11:19 < nmz787_i> talked to local lab, it will be fine to use some sq feet of their lab bench near their fume hood
11:19 < kanzure> actually i just got done sending treadwell an email a few moments ago
11:19 < nmz787_i> and also other table space or cube space even for optics
11:19 < kanzure> sgraber is in #dlp3dprinting:
11:19 < kanzure> oops i mean #dlp3dprinting
11:20 < nmz787_i> chemist or matSci
11:20 < nmz787_i> or semiconductor [whatever suffix here]
11:20 < kanzure> uv resin person
11:20 < kanzure> oh, i mean, sgraber is uv resin person
11:20 < kanzure> treadwell is anomalous
11:22 < ParahSailin> what you need a chemist for
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11:24 < nmz787_i> chemistry
11:24 -!- jmil_ [~jmil@hive76/member/jmil] has quit [Client Quit]
11:24 < nmz787_i> polymer chemsitry of different sorts
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11:25 < ParahSailin> trying to do novel chemistry?
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11:26 < nmz787_i> sort of munging
11:26 < kanzure> ParahSailin, it would be nice to have an organic synthesis person in here in general, for oligo synthesis reasons
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11:26 < ParahSailin> thats actually my degree
11:27 < nmz787_i> subprocess.py is importing _subprocess but I don't know where its coming from
11:27 < ParahSailin> nobody ever believes me when i weigh in on stuff though
11:27 < kanzure> never name your python files the same as python default modules -_-
11:27 < nmz787_i> ahh
11:27 < nmz787_i> is that compiled into the python.exe or some dll then?
11:28 < kanzure> you're asking me if a standard python library is a standard part of python?
11:28 < nmz787_i> no i'm asking can I find the source code in my Lib dir somewhere
11:28 < kanzure> .py import subprocess; subprocess.__path__
11:28 < yoleaux> AttributeError: 'module' object has no attribute '__path__'
11:28 < kanzure> .py import subprocess; subprocess.__file__
11:29 < kanzure> .py import subprocess; print subprocess.__file__
11:29 < yoleaux> /base/data/home/runtimes/python/python_dist/lib/python2.5/subprocess.py
11:29 < nmz787_i> yeah but in subprocess.py
11:29 < nmz787_i> "if mswindows:
11:29 < nmz787_i>     import threading
11:29 < nmz787_i>     import msvcrt
11:29 < nmz787_i>     import _subprocess"
11:30 < ParahSailin> _subprocess is gonna be the c module
11:30 < nmz787_i> ah http://stackoverflow.com/a/10326123/253127
11:30 < nmz787_i> what ParahSailin said
11:31 < nmz787_i> subprocess.wait() seems to be crashing
11:31 < nmz787_i> only the second time it's called though
11:31 < nmz787_i> at least the second time in that block of code
11:31 < nmz787_i> it is called elsewhere too
11:31 < nmz787_i> so maybe it's the 5th time its called
11:32 < nmz787_i> or 6th
11:32 < dingo> what ParahSailin says, its a C module if its _modulename
11:32 < dingo> i don't think you would call .wait() more than once
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11:32 < dingo> should return the exit status or some such
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11:34 < ParahSailin> did you strace
11:34 < nmz787_i> no i didn't install cygwin
11:36 < nmz787_i> someone recommends this http://technet.microsoft.com/en-us/sysinternals/bb896645.aspx
11:36 < kanzure> ParahSailin: have you done organic synthesis stuff, like distillation, fractionation, glassware-fu?
11:37 < kanzure> i have already spent too much of my life reading source code from sysinternals
11:38 < kanzure> .title http://chem.rochester.edu/nvdcgi/commercavail.cgi
11:38 < yoleaux> Should you buy it from a commercial supplier,or make it yourself?
11:41 < ParahSailin> kanzure: yeah and it all sucks
11:44 < kanzure> sucks more than bio?
11:44 < ParahSailin> yeah because you have to do apparatus shit
11:44 < ParahSailin> and weird temperatures
11:45 < ParahSailin> sometimes glovebox
12:11 < delinquentme> Is modifying a compound to allow it to be taken orally a "solved" process?
12:12 < kanzure> yes, you put it in a capsule
12:12 < delinquentme> does it just mean making it stable within a highly acidic environment
12:13 < ParahSailin> enteric coated capsule
12:17 < nmz787_i> delinquentme: there are enzymes and also blood brain barrier
12:17 < nmz787_i> enzymes as in ones that will break it down via gut
12:17 < nmz787_i> sometimes
12:17 < nmz787_i> and also a molecule than gets through gut, may not be in form to make it past bbb
12:18 < ParahSailin> im going to generously interpret what he was trying to ask here. can a large molecule be protected from the stomach other than by physical barrier? no not solved
12:18 < nmz787_i> delinquentme: they are researching nano encapsulation stuff
12:19 < kanzure> save your generosity
12:19 < ParahSailin> that was one act of generosity for one github commit
12:19 < delinquentme> ParahSailin, kanzure nobody has verified it even works.
12:19  * delinquentme glares
12:20 < ParahSailin> wait you didnt do a simple test of it in repl?
12:21 < delinquentme> ParahSailin, thats hardly considered a "test"
12:21 < delinquentme> run.dat.shit.
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12:21 < delinquentme> also tldr nobody seems to have a fucking clue or care how it works
12:22 < delinquentme> ask one question to kanzure have to re-ask it 10 times in different manners to get a useful answer
12:22 < delinquentme> ( w regards to novel paper fetching from nature )
12:22 < kanzure> paperbot: reload papers
12:22 < paperbot> kanzure: <module 'papers' from '/srv/ikiwiki/paperbot/modules/papers.py'> (version: 2013-07-09 18:45:35)
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12:23 < ParahSailin> ask the right question, get the answer to the question
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12:23 < ParahSailin> this how words work
12:23 < kanzure> delinquentme: or maybe i'm just not advertising the method because it's in my best interest not to
12:23 < kanzure> (because of obvious reasons0
12:24 < kanzure> suppose i was using a proxy installed on nih.gov
12:24 < delinquentme> sure
12:24 < kanzure> do you really think it's wise for me to say that in public -_-
12:24 < kanzure> you guys are all terrible people
12:24 < delinquentme> you've got my phone #
12:24 < kanzure> anyway, the source code is totally accurate and nothing is missing on github
12:24 < delinquentme> and im sure you have more than enough info to blackmail me
12:24 < kanzure> i would call you, except there is literally no missing component that you haven't seen
12:25 < delinquentme> I'm just conveying that there is a small even if loose circle of trust
12:25 < kanzure> erm, i mean, literally no component that you haven't seen
12:25 < kanzure> no magic
12:25 < delinquentme> at the time it seemed like it was
12:25 < kanzure> paperbot: reload papers
12:25 < paperbot> kanzure: papers: no such module!
12:26 < ParahSailin> im also virtually certain that the specifics have at one time or another been publicly discussed in this channel
12:26 < kanzure> huh where did the module go
12:26 < kanzure> whybreak?
12:26 < kanzure> Error loading scihub: [Errno 2] No such file or directory: 'libgen_login.txt' (in bot.py)
12:26 < kanzure> whodidthis
12:27 < delinquentme> ParahSailin, so I saw this lovely little paper http://www.nature.com/nchembio/journal/vaop/ncurrent/full/nchembio.1551.html  and it seems like it would be something of HUGE interest to both wetlab workers that I've got over here + generics manufacturers
12:27 < delinquentme> but currently at the " someone pay me for it " stage
12:28 < delinquentme> need pharma insiders
12:28 < ParahSailin> http://libgen.org/scimag/get.php?doi=10.1038%2Fnchembio.1551
12:28 < kanzure> why are we using a text file
12:28 < kanzure> git blame says it's delinquentme's fault
12:29 < delinquentme> thats what I decided to go w
12:29 < ParahSailin> you guys had a thing where you decided the secret genesis/upload lp doesnt belong in the repo
12:29 < ParahSailin> even though it is in plaintext on a russian hacker site
12:29 < kanzure> https://github.com/kanzure/paperbot/pull/29
12:30 < kanzure> i must have missed that part of the conversation
12:31 < delinquentme> it was in heeere
12:33 < delinquentme> emailed the creds file kanzure
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12:39 < gnusha_> https://secure.diyhpl.us/cgit/paperbot/commit/?id=c9bd6e7a Bryan Bishop: back to hardcoding a password?
12:39 < gnusha_> paperbot: reload papers
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12:41 < delinquentme> paperbot, worlddomination()
12:42 < delinquentme> ParahSailin, 3 commits. I want a hug.
12:42 < delinquentme> and a promise ring.
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13:06 < kanzure> https://github.com/dcowden/cadquery
13:07 < kanzure> huh, so it turns out that freecad's python modules can be used without running the freecad executable
13:07 < kanzure> and without using freecad's embedded python interpreter
13:08 < chris_99> i was using FreeCad recently thought it was pretty good, except when it crashed
13:08 < chris_99> (that could be because i was using the Windows version?)
13:10 < kanzure> no, it crashes everywhere
13:11 < kanzure> opencascade is crashy
13:11 < kanzure> that is why freecad crashes
13:11 < chris_99> ah
13:16 < delinquentme> le brb
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13:37 < kanzure> .wik PLaSM
13:37 < yoleaux> "PLaSM (Programming Language of Solid Modeling) is an open source scripting language for solid modeling, a discipline that constitutes the foundation of computer-aided design  and CAD systems. In contrast to other CAD programs, PLaSM emphasizes scripting rather than interactive GUI work." — http://en.wikipedia.org/wiki/PLaSM
13:37 < kanzure> http://www.plasm.net/
13:38 < kanzure> "1.  Plasm primitives are ALL CAPS (all capital letters)" ugh
13:39 < chris_99> weird
13:40 < kanzure> https://github.com/cvdlab/plasm-crumbs-slides/blob/master/chapters/surfaces/Readme.md
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13:49 < kanzure> # example of boolean intersection
13:49 < kanzure> Plasm.view(Plasm.boolop(BOOL_CODE_AND,[Plasm.cube(2,-0.5,0.5),Plasm.rotate(Plasm.cube(2,-0.5,0.5),2,1,2,pi/4)]))
13:49 < kanzure> probably refers to https://github.com/plasm-language/pyplasm/blob/master/src/xge/plasm.boolop.cpp
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13:51 < kanzure> or this one: https://github.com/femhub/plasm/blob/master/src/xge/plasm.boolop.cpp
13:52 < kanzure> looks like a polyhedral surface intersection meto
13:52 < kanzure> *method
13:52 < kanzure> oh well
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13:59 < kanzure> paperbot: http://www.ingentaconnect.com/content/asp/jctn/2008/00000005/00000005/art00002
13:59 < kanzure> .title
14:00 < kanzure> i don't remember seeing a 100 page paper about this
14:00 < yoleaux> A Minimal Toolset for Positional Diamond Mechanosynthesis: ingentaconnect
14:00 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/52b613cfcb8fb9744bf22452ed2828a1.txt
14:00 < kanzure> oh it was probably this one http://www.molecularassembler.com/Papers/MinToolset.pdf
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16:34 < nmz787_i> kanzure: for a second i thought that url was molecularembarraser.com
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16:42 < kanzure> http://gizmodo.com/5013018/boeing-successfully-fires-25-kw-solid-state-lasers-laser-weapons-one-step-closer-to-being-a-reality
16:42 < gradstudentbot> I already have one of those in my collection, you know.
16:44 < kanzure> 50 W laser diode for $500 http://www.alibaba.com/product-detail/high-performance-diode-laser-808nm-50W_1219796601.html
16:45 < chris_99> that seems really cheap
16:45 < chris_99> are they more or less reliable than CO2?
16:46 < kanzure> no clue
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17:36 < streety> something doesn't add up there ... max power 50W, max forward current 1.2A, max forward voltage 2.2V
17:36 < streety> only two of those can be correct
17:36 < chris_99> haha yeah
17:38 < ThomasEgi> streety, lasers?
17:38 < gene_hacker> 50 watts pulsed probably
17:38 < gene_hacker> yup 808 nm, that's probably a range finding laser
17:39 < ThomasEgi> 808 doesn't sound like CO2 tho
17:39 < chris_99> it's a diode
17:39 < ThomasEgi> diode laser?
17:39 < gene_hacker> yes
17:39 < chris_99> yeah scroll up
17:40 < gene_hacker> http://www.repairfaq.org/sam/laserdio.htm#diocss7b
17:41 < chris_99> ah
17:41 < chris_99> interesting
17:42 < chris_99> can you get non-pulsed high power laser diodes
17:43 < ThomasEgi> those are usualy arrays tho.
17:43 < kanzure> that's unfortunate
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17:51 < chris_99> http://www.ebay.com/itm/50W-CW-Diode-Pumped-Nd-YAG-Cavity-DPSS-Laser-Module-/270605121972?pt=LH_DefaultDomain_0&hash=item3f015279b4
17:56 < gene_hacker> now if we could only manage to make an array of phase locked laser diodes...
17:57 < kanzure> "Buyers must be 18 years old"
17:58 < kanzure> that is an oddly specific target market..
17:58 < kanzure> 18 year olds that want 50 W solid state lasers
17:58 < chris_99> haha
17:59 < ThomasEgi> they won't phase lock
17:59 < ThomasEgi> but you could use them to pump another laser
18:00 < ThomasEgi> wait. that IS a diode pumped laser
18:00 < gradstudentbot> If you help me, I'll put you on the paper.
18:01 < ThomasEgi> those numbers don't make sensetho.
18:02 < chris_99> what numbers?
18:02 < ThomasEgi> current, voltage, power
18:02 < ThomasEgi> iirc nd-yag have typical efficiencies around 1% or so.
18:02 < nmz787_i> on the ebay page?
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18:03 < ThomasEgi> yup
18:03 < nmz787_i> ThomasEgi: it might make sense if they're including the non-central output freqs
18:04 < chris_99> http://www.sino-laser.com/pdf/GN50_spec.pdf
18:05 < chris_99> not much more info
18:06 < nmz787_i> has some higher numbers
18:06 < ThomasEgi> well it's not entirely off.
18:07 < ThomasEgi> given it's diode laser pumped its efficiency might be a bit higher than traditional flash tube pumping
18:07 < ThomasEgi> now.. time for sleep. exams tomorrow.. coincidently.. an exam about lasers
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18:14 < gene_hacker> they won't phase lock yet
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21:13 < kanzure> paperbot: http://pubs.acs.org/doi/abs/10.1021/ed083p919
21:13 < kanzure> .title
21:13 < yoleaux> Student Empowerment through 'Mini-microscale' Reactions: The Epoxidation of 1 mg of Geraniol
21:13 < paperbot> http://libgen.org/scimag/get.php?doi=10.1021%2Fed083p919
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21:19 < ||0_-_0||> paperbot http://www.nature.com/nbt/journal/v25/n11/abs/nbt1356.html
21:19 < kanzure> http://libgen.org/scimag/get.php?doi=10.1038%2Fnbt1356
21:20 < paperbot> http://libgen.org/scimag/get.php?doi=10.1038%2Fnbt1356
21:34 < ||0_-_0||> paperbot http://link.springer.com/article/10.1007/BF02462863
21:34 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/588841dac2dc9487050294e016b6b61a.txt
21:47 < nmz787_i> i wonder if there are places around here that do etching as a service
21:48 < nmz787_i> if the microscope + blu-ray works to make a photoresist mask... then I'd think it would work for etching protection with some process or another
21:48 < nmz787_i> in silicon
21:48 < nmz787_i> or glass
21:49 < gradstudentbot> Should I be doing this in the fume hood?
21:54 < kanzure> naaah it'll be fine, radstudentbot
22:00 < gene_hacker> I think you should listen to it, it has a valid point
22:00 < kanzure> fumes build character
22:00 < gene_hacker> at least use a fan or something, like those diy fume extractors
22:02 < gene_hacker> and did you ever figure out how that group in diybio is going to build a synth?
22:02 < gene_hacker> what are they using magnetic beads for?
22:02 < kanzure> haven't received any information from them
22:02 < ||0_-_0||> worth a try
22:02 < ||0_-_0||> paperbot http://link.springer.com/article/10.1007%2Fs10516-007-9011-2?LI=true
22:02 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/2048d088a483941d4398cbfbe226d883.txt
22:03 < gene_hacker> I wonder if they'll be able to do a kilobase, because if they do, things could get interesting...
22:04 < kanzure> we could do the picoarray setup
22:05 < kanzure> haven't found a vendor for the photogenerated acid inputs
22:05 < kanzure> (haven't looked, though)
22:06 < gene_hacker> I think it's shouldn't be too hard to find
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22:06 < kanzure> .wik luer-lock joint
22:06 < yoleaux> "A syringe is a simple pump consisting of a plunger that fits tightly in a tube. The plunger can be pulled and pushed along inside a cylindrical tube (called a barrel), allowing the syringe to take in and expel a liquid or gas through an orifice at the open end of the tube. The open end of the syringe may be fitted with a hypodermic needle, …" — http://en.wikipedia.org/wiki/Syringe
22:06 < kanzure> what just happened
22:07 < gene_hacker> anyway, I'm wondering some stuff about diamonoid mechanosynthesis
22:07 < gene_hacker> just how stiff a manipulator one needs to do it
22:10 < kanzure> well, you need to survive jamming it into surfaces randomly (crashing the tip)
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22:10 < gene_hacker> easy enough
22:13 < kanzure> have you read the tooltip paper? it might be in there
22:13 < kanzure> many things might be in there..
22:13 < gene_hacker> I'll have a look
22:13 < kanzure> in http://www.molecularassembler.com/Papers/MinToolset.pdf
22:14 < kanzure> page 764 mentions compressive loads and stiffness..
22:16 < gene_hacker> excellent
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22:17 < gene_hacker> I think that's just the tool tip
22:18 < kanzure> pffft so demanding :)
22:19 < gene_hacker> I'll find it somewhere
22:19 < kanzure> .wik luer taper
22:19 < yoleaux> "The Luer taper is a standardized system of small-scale fluid fittings used for making leak-free connections between a male-taper fitting and its mating female part on medical and laboratory instruments, including hypodermic syringe tips and needles or stopcocks and needles." — http://en.wikipedia.org/wiki/Luer_taper
22:20 < kanzure> paperbot: http://www.iso.org/iso/iso_catalogue/catalogue_tc/catalogue_detail.htm?csnumber=4693
22:21 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/5426d8a03004c94623bf35f7b939f1bc.txt
22:21 < kanzure> damn
22:25 < kanzure> the photogenerated acid paper used "biaryl iodium PGA-P (Secant Chemicals) (3% in CH2Cl2) and photosensitizing thioxanthenone (Sigma-Aldrich). The solution was protected from light during preparation and storage."
22:26 < kanzure> "In a PGA deprotection step, droplet formation was achieved by filling the reactor from bottom to top with PGA-P solution, and then draining the solution from the bottom of the reactor. The flow direction was controlled by a four-way electronic valve (Valco)."
22:26 < kanzure> "The light-directed acid deprotection steps lasted 0.5 - 2 min and were followed by a rapid pyridine/CH3CN (10/90 v/v) wash. The rest of the synthesis steps were coupling, capping and oxidation and they were the same as performed in conventional oligonucleotide synthesis."
22:27 < kanzure> "coupling reaction using a mixture 1:10 of fluorescein:T phosphoramidites" sounds nice
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22:30 < kanzure> hm, their microarray had wells with a diameter of 150-600 microns. that's pretty big.
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22:36 < gene_hacker> well within the range of what can be done with a projector
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22:39 < gene_hacker> the hard part is optimizing the chambers for equal flow
22:39 < kanzure> i'm not sure it needs to have chambers
22:39 < gene_hacker> but that shouldn't be too hard with
22:39 < gene_hacker> true
22:39 < kanzure> what were the chambers for?
22:40 < gene_hacker> isolating the photogenerated acid
22:41 < gene_hacker> preventing it from uncapping that which you don't want uncapped
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22:51 < kanzure> wouldn't that happen during the wash cycle? the acids would get pushed around to the other chambers.
22:51 < gene_hacker> I don't know
22:52 < gene_hacker> now where was that paper again?
22:53 < kanzure> which one would you like
22:53 < gene_hacker> http://www.lcsciences.com/wp-content/uploads/picoarray.png
22:53 < gradstudentbot> We were out of the right dye, so I just used an equivalent.
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22:53 < gene_hacker> nope it's simpler than that
22:53 < kanzure> http://diyhpl.us/~bryan/papers2/microfluidics/synthesis/Synthesis%20-%20Microfluidic%20PicoArray%20synthesis%20of%20oligodeoxynucleotides%20and%20simultaneous%20assembling%20of%20multiple%20DNA%20sequences%20(10%20kb).pdf
22:53 < gene_hacker> there are dedicated waste channels
22:53 < gene_hacker> take a look a the arrows on the picture
22:54 < kanzure> looks like all the same channel to me
22:54 < gene_hacker> look closer
22:55 < gene_hacker> the acid gets washed out into the waste channels
22:55 < kanzure> osnap you're right
22:55 < gene_hacker> not into other chambers
22:55 < kanzure> those waste channels are not connected to the main inlet
22:55 < kanzure> huh
22:55 < gene_hacker> correct
22:55 < gene_hacker> they are connected to the outlet
22:55 < kanzure> yep i totally missed that
22:56 < gradstudentbot> It's not really significant, but there's definitely a trend.
22:57 < gene_hacker> and that's why you have to carefully optimize the channels
22:57 < gene_hacker> of course it's low Re flow, it can't be that bad
22:57 < kanzure> waste channel could be deeper, maybe
22:58 < gene_hacker>  or 3d
22:58 < gene_hacker> however, aren't the chemicals needed nasty solvents?
23:01 < kanzure> nmz787 has been looking into material compatibility things
23:02 < gene_hacker> now I was going to say ceramic, but ceramic isn't clear
23:02 < gene_hacker> no wait, it can be
23:03 < gene_hacker> it's just insane: http://en.wikipedia.org/wiki/Transparent_ceramics
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--- Log closed Fri Jun 20 00:00:40 2014