--- Log opened Sun Apr 12 00:00:50 2015
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05:07 < Adlai> maaku: it, it, ... them?
05:07 < Adlai> :D
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07:09 < chris_99> Does anyone know of any studies comparing a stir bar vs an orbital shaker for things like growing yeast
07:09 < yoleaux> 11 Apr 2015 00:57Z <nmz787> chris_99: ah, cool! what are you gonna use to control the CCD?
07:09 < chris_99> or have any options
07:10 < chris_99> !help
07:10 < chris_99> hmm, how do i leave a message with yoleaux too heh
07:11 < cluckj> I haven't seen stir bars used for growing yeast on a small scale
07:12 < chris_99> oh, they're used a lot in the homebrewing world, but im wondering if a shaker would be the same in performance
07:12 < cluckj> stir bars tend to be attached to hot plates, and orbital shakers tend to also be incubators
07:12 < cluckj> oh
07:12 < chris_99> i've found an orbital shaker with a hotplate
07:14 < chris_99> but i'm not sure if i should get a stir  plate intead
07:14 < cluckj> why not both?
07:15 < chris_99> heh, i don't have much spare cash atm
07:15 < cluckj> oh hah
07:16 < cluckj> I think it might be a scale issue....an orbital shaker that can do a 15 gallon drum of beer is gonna be scary as fuck
07:18 < chris_99> oh to make it clear, this is just for a yeast starter
07:18 < chris_99> so only say 1litre or 2litre
07:21 < chris_99> although a 15 gal one would be cooler :)
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07:28 < cluckj> haha
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08:05 < Adlai> paperbot: http://pubs.rsc.org/en/Content/ArticleLanding/2013/CS/c3cs35433d
08:06 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/New%20insights%20into%20prebiotic%20chemistry%20from%20Stanley%20Miller%27s%20spark%20discharge%20experiments.pdf
08:07 < Adlai> esgeht!
08:14 < Adlai> paperbot: http://onlinelibrary.wiley.com/doi/10.1002/anie.198308161/pdf
08:14 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/825c1ef0c098892697e4bd594410ed76.txt
08:14 < kanzure> hmph
08:14 < Adlai> hmm.
08:15  * Adlai should get to "work" anyway
08:15 < kanzure> bah
08:15 < kanzure> or file bug reports on paperbot
08:15 < kanzure> https://github.com/kanzure/paperbot
08:21 < Adlai> ah, but https://github.com/kanzure/paperbot/issues/22 already exists :P
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08:30 < catern> https://mchankins.wordpress.com/2013/04/21/still-not-significant-2/
08:30 < catern> lol
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08:32 < Adlai> "For example, “a trend towards significance” expresses non-significance as some sort of motion towards significance, which it isn’t: there is no ‘trend’, in any direction, and nowhere for the trend to be ‘towards’"
08:37 < CheckDavid> What do you guys think of fasting?
08:38 < Adlai> leeches have also been said to cure numerous ailments
08:40 < kanzure> CheckDavid: also consider tapeworms
08:41 < CheckDavid> You drawing an analogy between leeches and fasting Adlai ?
08:43 < Adlai> yes. are you thinking of fasting? the answer to "why or why not" either makes my analogy relevant or irrelevant... fasting "done right" probably has more psychological benefit than leeches
08:43 < FourFire> CheckDavid, Honey as disinfectant
08:43 < CheckDavid> I did already a 2 day fasting
08:43 < CheckDavid> Oh heard that one before FourFire
08:43 < FourFire> Do you understand the mechanism behind it?
08:44 < CheckDavid> FourFire: fasting?
08:44 < CheckDavid> No I am not aware of the mechanics.
08:44 < kanzure> whoops i mean tapeworm eggs
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08:45 < CheckDavid> Do you FourFire ?
08:45 < kanzure> i don't even know if non-egged tapeworms work
08:45 < CheckDavid> They work very hard man.
08:47 < FourFire> CheckDavid, no honey
08:47 < CheckDavid> I read some shit online about fasting and was very intrigued
08:47 < cluckj> there is some dude whose lab I wanted to get into who was grinding up whipworms and giving them to people
08:47 < cluckj> to cure a whole bunch of autoimmune conditions
08:47 < CheckDavid> People reporting feeling energetic after 9 days of nutrient deprivation and sucb
08:47 < CheckDavid> I had to try it
08:48 < CheckDavid> That's how intrigued I was
08:48 < CheckDavid> *such
08:48 < CheckDavid> But only for like 60 hours
08:48 < CheckDavid> Lil over 48 hours
08:48 < CheckDavid> Barely a proper experience
08:48 < cluckj> starvation conditions change your biochemistry
08:49 < CheckDavid> But still interesting
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08:49 < CheckDavid> cluckj: can you elaborate?
08:50 < cluckj> CheckDavid, your body basically empties out its local energy storage, then starts consuming functional proteins, then your fat cells dump all their storage
08:50 < Adlai> when an animal starves, it ceases storing fat, and begins digesting stored fat
08:50 < cluckj> there are some diets that can forcce your body into that kind of mode tho
08:50 < Adlai> iiuc you don't start consuming functional proteins until you're in quite a dilipidated state
08:50 < cluckj> keto diets do that, where you put yourself into a state of ketogenesis
08:51 < cluckj> Adlai, I thought so too but iirc your body does the intercellular stuff first
08:53 < Adlai> fasting also makes you metabolize more rapidly once you ingest, so it's also used to accelerate/intensify psychoactive effects
08:53 < cluckj> yeah
08:54 < cluckj> when you do the keto diet, if you eat too many carbs you ruin the ketogenic state
08:54 < CheckDavid> Some say muscle goes first
08:54 < CheckDavid> Others say fat goes first
08:54 < CheckDavid> Which is more accurate?
08:55 < cluckj> I think muscle does
08:55 < cluckj> in starvation conditions
08:55 < cluckj> it's stupid but the human body was not intelligently designed :)
08:55 < cluckj> and it's a temporary state
08:56 < cluckj> it's not like you run out of muscles before your body starts eating fat stores
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08:56 < kanzure> there should be a muscle eating starvation curve chart
08:56 < cluckj> there probably is
08:58 < cluckj> from my own personal experience, muscle is definitely higher on the consuming/producing curve than fat
09:01 < cluckj> I've been trying to gain some fat back from the undiagnosed diabetes starvation conditions for like 2 years, and all I can get is useless shitty muscle
09:01 < cluckj> (I need fatty places to stick cannulas and sensors)
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10:03 < CheckDavid> damn that's crazy cluckj
10:03 < CheckDavid> Some people will be envious
10:04 < CheckDavid> Like me
10:04 < CheckDavid> I'm envious that you get so much useless muscle
10:04 < CheckDavid> And no fat
10:04 < cluckj> lol
10:05 < cluckj> it was because of undiagnosed type 1 diabetes
10:06 < cluckj> so there's a tradeoff, I guess :P
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10:20 < kanzure> "Visualize black hole gravitational lensing in WebGL" https://github.com/cliffcrosland/black-hole.js
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10:36 < CheckDavid> Oh I see
10:38 < CheckDavid> cluckj: I laugh at women who think they will get very large by lifting weights. They are afraid of.getting too much useless muscle
10:38 < CheckDavid> Like you
10:38 < CheckDavid> But then I know you are not a women because else you wouldn't even be putting on too much muscle
10:38 < CheckDavid> Lol
10:39 < CheckDavid> That's the funny thing
10:40 < cluckj> O_o
10:42 < kanzure> uh...
10:43 < kanzure> wat
10:43 < cluckj> ^
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10:56 < kanzure> "They had the brain open, and every single time that they had an electrode stimulation to a very certain region of the cardiac nucleuses, which is part of this wanting system, the patient would turn around and say that he loved the doctor."
10:57 < andytoshi> that doctor sounds lovely
10:58 < kanzure> no you're supposed to wait until he zaps you
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10:59 < andytoshi> oh, ok
10:59 < andytoshi> i can't wait :]
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11:55 < nmz787> this looks pretty friggin cool http://www.learnopencv.com/seamless-cloning-using-opencv-python-cpp/
11:55 < chris_99> intriguing
11:56 < chris_99> but i guess it only works if the object has a solid background
12:09 < chris_99> i'm still deciding on the ADC, nmz787, maybe i should just go for something cheap to start with
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13:01 < nmz787> chris_99: have you checked the openspectrometer specs?
13:01 < nmz787> the ADC isn't outlandishly priced
13:02 < chris_99> yeah that seemed a good one, the wolfson one
13:03 < chris_99> if i just used one of those 'slide' diffraction gratings, i can just place it straight in front of the ccd right
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13:43 < nmz787> by slide you mean transmission?
13:43 < chris_99> yeah
13:43 < nmz787> yep, check out the PLOTS spectrometer to see their setup instructions
13:43 < chris_99> ah cheers
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14:39 < kanzure> vivi: hi
14:40 < vivi> hello kanzure
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16:17  * Adlai , being drunk, is now curious to solicit the channel's opinion of alcohol
16:17 < Adlai> it's definitely not nootropic
16:17 < Adlai> subhuman, and by muddling the conscious/precise mind, it interferes with bio- and hardware hacking - thus a perfect fit for this channel!
16:19 < kanzure> lobotomies are also not often nootropic
16:20 < Adlai> sure, but lobotomies don't write poetry, or cause desperate monkeys to ask other monkeys what they think of monkeydom
16:21 < Adlai> (and neither do lobotomized monkeys, they tend to sit around, staring at wallS)
16:27  * Adlai has been pondering the value of a university education
16:27 < Adlai> some of the last words out of my mouth were
16:28 < Adlai> "i have found a bunch of people doing independent biology, so even that isn't restricted to the hallowed groves of academe anymore"
16:28 < Adlai> prove me wrong, please
16:28 < Adlai> (actuallly, i'd rather you don't, but i think you know what you mean. yes, _you_)
16:30 < cluckj> :(
16:30 < cluckj> me?
16:31 < kanzure> Adlai: friends don't let friends go to school
16:31 < cluckj> hah
16:32 < Adlai> o rly
16:32 < Adlai> y not?
16:32  * Adlai means, that the last friends - with whom he spoke about the last words whence his mouth - urged him to study
16:32 < kanzure> learning can happen anywhere, and you can join labs without being a student
16:32 < Adlai> and by "study", /me implies at a formal institution
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16:34 < kanzure> and besides, you're a programmer. your industry doesn't even care about credentials.
16:35 < cluckj> I wonder why that is
16:35 < Adlai> eh it only disregards credentials when your github is full of nodejs angular blabla
16:35  * Adlai managed the "i don't have a resume but github" once, twas nice, nevar again
16:35 < kanzure> nobody actually reads source code on github when a candidate applies. they really don't...
16:36 < kanzure> (but yes you should still have shit there)
16:37 < Adlai> dunno
16:37 < Adlai> i heard "he thought your github was nice" in the first skype call
16:38 < Adlai> doesn't that _mean_ something?
16:39 < kanzure> it could easily mean nothing, like "he said something positive, but i don't understand the gitz, so i am calling you to waste your time anyway instead of just having him review the quality of source code you write"
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17:08 < andytoshi> kanzure: actually i received a PM from an employer (of others) complaining that github made it hard to tell whether contributions are "real"
17:08 < andytoshi> that is, code rather than docfixes
17:08 < andytoshi> so he/she is at least looking as far as what shape are git commits
17:08 < andytoshi> but maybe the people who PM me about their hiring troubles are not typical employers. *shrug*
17:09 < Adlai> andytoshi: IME, PMs are from recruiters
17:09 < Adlai> (for values of personal increasingly tending towards "linked")
17:11 < andytoshi> Adlai: fyi, people keep irc logs forever ... it's kinda fun but i make a point of never being on IRC while drunk for that reason
17:11 < andytoshi> (not that you've said anything worrisome, just general advice)
17:11  * Adlai has seen far worse
17:12 < Adlai> some IRC networks are built around drugs. imagine how #stims on irc.tripsit.me looks...
17:13 < andytoshi> sure, but if you're looking to do research/development stuff under this name with people on this network, there's a benefit to being more cautious. *shrug again*
17:13 < kanzure> andytoshi: git commits can be forged too, but whatever
17:14  * Adlai shuts up?
17:17 < andytoshi> kanzure: this is true, but i think the skill needed to realize that is also a barrier..
17:18 < andytoshi> idk, maybe it'd be more common if it was more useful
17:18 < andytoshi> right now you can post "trololol" in the Issues section of a million projects and github will say you've "contributed to" them all
17:18 < Adlai> yes, but forging _history_ is more difficult
17:19 < Adlai> (and requires getting pulled, or convincing your potential employers that your nonsense really is worth something)
17:20 < andytoshi> Adlai: well, you need push access to whatever repo you want that history to be in...but the actual forgery is easy enough
17:20 < andytoshi> like you can just put your name on every commit
17:20  * Adlai has several times told himself that correcting trailing whitespace in widely used open source projects is not worth the github profile boost that it would yield
17:21 < Adlai> please, show me the recruiter than would notice.
17:34 < andytoshi> btw kanzure, it took me all of five minutes to set up a new server running modern software (i'm migrating files right now), thx for the push to do it..
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17:37 < kanzure> cool
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17:55 < kanzure> "Before bitcoin [ransomware] was done with Moneypack, Western Union, and others"
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18:46 < kanzure> somewhat more popularized fermi paradox explanation post http://waitbutwhy.com/2014/05/fermi-paradox.html
18:49 < adlai> we have no way of directly inspecting "the multiverse"... maybe "life" (ie consciousness capable of conceiving such paradoxen) is much much rarerererer than expected, and by the time/verse it arises, is alone to wonder about this?
18:49 < kanzure> adlai: one interesting theory is that binary pulsars are probably alien civilizatios
18:49 < kanzure> alien civilizations
18:50  * adlai hopes that any aliens capable of reaching us with signals would be capable of a) being reached by ours, and b) understanding how to adapt their signals to be parsable by our admittedly humble intelligence
18:51 < adlai> it could also be that "nobody cares", it's a simulation/anomaly/whateveryoumakeofit
18:51 < adlai> these are all equivalent, from within.
18:53 < adlai> maybe i'm expecting the communication counterparty to slow down for my benefit, a sin equal to expecting your audience to "keep up" (an accusation recently levelled against an author using too much math in a paper intended for mathematicians)
18:54 < adlai> ... but why shouldn't non-interplanetary worms on a rock in space hope that their elders give them a helping hand?
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19:13 < kanzure> http://www.piratehearts.com/blog/2014/03/28/crt-simulation/
19:18 < streety> paperbot http://www.sciencedirect.com/science/article/pii/S0956566315002018
19:18 < kanzure> .title
19:18 < yoleaux> Microfluidic enzymatic biosensing systems: A review
19:19 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/%0A%20Microfluidic%20enzymatic%20biosensing%20systems%3A%20A%20review%0A%20.pdf
19:19 < kanzure> paperbot: http://www.sciencedirect.com/science/article/pii/S0956566315002018/pdfft?md5=74aa65b3289041cdf3bdd4154bb8a5fb&pid=1-s2.0-S0956566315002018-main.pdf
19:19 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/6062d8ae911843d1978167384447d54a.pdf
19:21 < streety> thanks
19:34 < adlai> kanzure: that crt simulation is skeumorphism taken to an impressive/worrying extreme
19:35 < kanzure> isn't that the channel moto? "take everything to worrying extremes"/
19:35 < adlai> /motto
19:35 < kanzure> ah yes
19:36  * adlai must've lepht something behind
19:36 < kanzure> ugh that person?
19:36 < adlai> dunno, you said worrying extreme
19:36 < kanzure> lepht is a person
19:37 < adlai> who, according to links from /motto, is extreme
19:40 < kanzure> uh?
19:40 < adlai> well i guess it's not linked from teh topic, but from the wiki.
19:42 < kanzure> she's just pandering to a boring audience who don't understand magnets or engineering
19:43 < adlai> them's a she now?
19:44  * adlai means no jest, trying to make sense
19:44 < kanzure> lepht is a person
19:44 < kanzure> her audience is a group of people
19:45 < adlai> ok
19:45  * adlai wonders if any biohackers have tackled sleep yet, or is that left for after the BRAIN initiative
19:59 < kanzure> introducing the most boring tool for scientific pdfs http://www.docear.org/ (really, this makes no sense. sounds super boring.)
20:27 < kanzure> .title https://news.ycombinator.com/item?id=9334316
20:27 < yoleaux> The future of the postdoc | Hacker News
20:35 < cluckj> tenure or bust
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--- Log closed Mon Apr 13 00:00:51 2015