--- Log opened Sat Jul 11 00:00:07 2015 00:11 -!- Acty [uid89656@gateway/web/irccloud.com/x-grukslkymwqluihs] has quit [Quit: Connection closed for inactivity] 00:42 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Quit: Leaving] 00:45 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 01:06 -!- ebowden [~ebowden@101.177.244.210] has joined ##hplusroadmap 01:16 -!- ParahSailin_ [~parahsail@unaffiliated/parahsailin] has quit [Remote host closed the connection] 01:28 -!- ParahSailin [~parahsail@unaffiliated/parahsailin] has joined ##hplusroadmap 01:47 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap 01:59 -!- Acty [uid89656@gateway/web/irccloud.com/x-ebhknhxflpoktmeo] has joined ##hplusroadmap 02:34 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-mpkndftiedlwfxnu] has joined ##hplusroadmap 02:34 -!- ebowden [~ebowden@101.177.244.210] has quit [Read error: Connection reset by peer] 03:16 -!- Quashie_ [~boingredd@50.14.92.17] has joined ##hplusroadmap 03:19 -!- Quashie [~boingredd@50.14.92.17] has quit [Ping timeout: 256 seconds] 03:31 -!- Porb [~Porbus@CPE-124-180-167-154.lns6.lon.bigpond.net.au] has joined ##hplusroadmap 03:51 -!- Porb_ [~Porbus@CPE-124-180-167-154.lns6.lon.bigpond.net.au] has joined ##hplusroadmap 03:52 -!- Porb [~Porbus@CPE-124-180-167-154.lns6.lon.bigpond.net.au] has quit [Ping timeout: 248 seconds] 04:10 -!- knobuddy [~Sayh@unaffiliated/knobuddy] has joined ##hplusroadmap 04:27 -!- proofoflogic [uid65184@gateway/web/irccloud.com/x-vwqlrtnecklyqnsk] has quit [Quit: Connection closed for inactivity] 04:30 -!- drewbot [~cinch@ec2-54-144-252-95.compute-1.amazonaws.com] has quit [Remote host closed the connection] 04:30 -!- drewbot [~cinch@ec2-54-166-28-217.compute-1.amazonaws.com] has joined ##hplusroadmap 04:36 -!- ebowden [~ebowden@101.177.244.210] has joined ##hplusroadmap 05:14 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 06:25 < ParahSailin> i got two mouse chips from affy 06:25 < ParahSailin> next time i go on site i need to go upstairs and grab a bunch of human ones 06:26 < ParahSailin> genechip mouse genome 430 2.0 array if anyone's curious 06:26 < ParahSailin> not sure if this is scrap or viable 06:30 < ParahSailin> hm, i gotta have them genotype my family as a fringe benefit 06:31 < kanzure> ParahSailin: can you possibly claim that you have a very very large extended family 06:32 < ParahSailin> they should have known that before they gave a guy with an irish name a job 06:32 < ParahSailin> their problem for not catching that sooner 06:33 < ParahSailin> i actually have no idea how a microarray is actually run 06:34 < ParahSailin> so what i need looks like the genome wide human 6.0 array 06:36 -!- ebowden [~ebowden@101.177.244.210] has quit [Remote host closed the connection] 06:37 -!- ebowden [~ebowden@101.177.244.210] has joined ##hplusroadmap 06:38 -!- ebowden [~ebowden@101.177.244.210] has quit [Remote host closed the connection] 06:38 < ParahSailin> kanzure: you ever worked with svitla in the ukraine? 06:39 -!- ebowden [~ebowden@101.177.244.210] has joined ##hplusroadmap 06:41 < kanzure> ParahSailin: i don't think so 06:42 < kanzure> "the flow of blood (heartbeats) are visible in brain tissue" 06:46 < kanzure> ""In Self/Less, a science-fiction thriller to be released in the U.S. today, July 10, 2015, Damian Hale, an extremely wealthy aristocrat (Ben Kingsley) dying from cancer, undergoes a $250 million radical medical procedure at a lab called Phoenix Biogenic in Manhattan to have his consciousness transferred into the body of a healthy young man"" 06:46 < kanzure> eh i'll probably skip that 06:46 < ParahSailin> wow lame, our human chip has less than a million snps? no wonder illumina eating our lunch 06:48 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 248 seconds] 06:49 < kanzure> maybe there are patent problems? 06:49 < ParahSailin> nah, they just got lazy 06:52 < ParahSailin> over ten years of no competition 06:54 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 06:56 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 07:02 < ParahSailin> damn, they have no goat panel 07:17 -!- ebowden [~ebowden@101.177.244.210] has quit [Remote host closed the connection] 07:25 < eleitl> kanzure, trying to get latest LG sources 07:25 < eleitl> requires manual intervention, everything online is stone old 07:31 -!- eudoxia [~eudoxia@r167-56-99-149.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 07:43 -!- Beatzebub [~beatzebub@d108-180-217-85.bchsia.telus.net] has quit [Quit: Beatzebub] 07:47 -!- ebowden [~ebowden@101.177.244.210] has joined ##hplusroadmap 07:54 -!- ebowden [~ebowden@101.177.244.210] has quit [Ping timeout: 256 seconds] 07:58 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-mpkndftiedlwfxnu] has quit [Quit: Connection closed for inactivity] 08:02 < JayDugger> Good luck, eleitl. 08:26 -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-uvgusbccgjrkokzv] has quit [Quit: Connection closed for inactivity] 08:37 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Quit: Namaste ] 08:54 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-sexmuyfeosqkmbla] has joined ##hplusroadmap 08:55 -!- eudoxia [~eudoxia@r167-56-99-149.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] 09:10 -!- FourFire [~FourFire@82.146.86.195] has joined ##hplusroadmap 09:25 -!- Porb_ [~Porbus@CPE-124-180-167-154.lns6.lon.bigpond.net.au] has quit [Ping timeout: 256 seconds] 09:32 -!- dustinm [~dustinm@105.ip-167-114-152.net] has quit [Ping timeout: 248 seconds] 09:32 < eleitl> Got the sources. 09:32 < eleitl> Thanks, JayDugger. 09:33 < eleitl> Shoveling manure and source code. 09:33 -!- dustinm [~dustinm@2607:5300:100:200::160d] has joined ##hplusroadmap 09:35 < eleitl> I think LG could use the loving attention of a good dev. 09:46 -!- zadock [~outsider@81.180.210.87] has joined ##hplusroadmap 09:56 < kanzure> their source code has always been bad 10:06 < kanzure> i am also not completely convinced that they are keeping good metadata 10:06 < kanzure> also, i wonder if set builders are faster than carpenters or machinists 10:10 -!- ebowden [~ebowden@101.177.244.210] has joined ##hplusroadmap 10:15 -!- ebowden [~ebowden@101.177.244.210] has quit [Ping timeout: 264 seconds] 10:43 < CaptHindsight> plenty of room in the FPGA on the MESA card for inkjet control 10:49 -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-ppnzfanjvxzgmess] has joined ##hplusroadmap 11:10 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has quit [Quit: gone] 11:11 < archels> potential here for cognitive enhancement? http://www.newscientist.com/article/dn27877-burst-of-light-speeds-up-healing-by-turbocharging-our-cells.html 11:13 < knobuddy> try shining a flaslight in ur ear 11:13 < yoleaux> 3 Jul 2015 03:41Z knobuddy: I think this is the closest thing to that far-side comic that I guess (and hope) is a joke http://www.theallium.com/policy/taylor-swift-announces-she-will-no-longer-review-for-nature/ 11:14 < knobuddy> i guess that's an offline msg relay via bot 11:17 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap 11:40 -!- CyberEater [~Genesteal@c-69-255-209-151.hsd1.md.comcast.net] has joined ##hplusroadmap 11:54 -!- _0bitcount [~Big_Byte@85.251.242.3.dyn.user.ono.com] has joined ##hplusroadmap 12:04 -!- nmz787_i [~ntmccork@134.134.139.77] has joined ##hplusroadmap 12:08 < nmz787_i> CaptHindsight: might check into the brand-name septum-seal called SureSeal 12:10 < CaptHindsight> nmz787: well the suppliers would have to adopt it or we'd have to repackage it 12:12 < CaptHindsight> http://blog.chembark.com/2013/08/04/hacks-for-septa/ 12:17 < CaptHindsight> http://www.chemglass.com/product_view.asp?pnr=AF-0522 12:17 < CaptHindsight> the HP TIJ cartridges use a bladder to hold the ink 12:18 -!- knobuddy [~Sayh@unaffiliated/knobuddy] has quit [Remote host closed the connection] 12:20 < CaptHindsight> if you don't push the needles through the seals in the reagent bottles until the printer enclosure is flooded with Argon that should also solve the problem 12:43 -!- nmz787_i [~ntmccork@134.134.139.77] has quit [Quit: Leaving.] 12:59 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 248 seconds] 13:00 < CaptHindsight> http://www.opentrons.com/ any automation engineer could have made this 13:01 < CaptHindsight> do workers in Bio labs not talk to engineers in other tech? 13:07 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 13:10 -!- delinquentme [~delinquen@99-8-186-132.lightspeed.snfcca.sbcglobal.net] has joined ##hplusroadmap 13:15 -!- proofoflogic [uid65184@gateway/web/irccloud.com/x-rfxfayubzuxtmoou] has joined ##hplusroadmap 13:15 < juri_> no, the communication methods seem to be radically different. 13:16 -!- CyberEater [~Genesteal@c-69-255-209-151.hsd1.md.comcast.net] has quit [Ping timeout: 264 seconds] 13:16 < juri_> I have been trying to help whe baltimore underground science space, but they don't use a mailing list, or IRC. 13:18 < CaptHindsight> I bet that they reinvented G-code as well 13:19 < CaptHindsight> heh, they are need the original makerbot startup space 13:19 < CaptHindsight> need/near 13:19 < streety> mailing lists seem quite common in bio labs 13:20 < streety> perhaps a last resort beyond a certain scale though 13:21 < streety> the DC diybio group only has a meetup page as yet 13:21 < CaptHindsight> I was just looking through a list of diybio groups 13:22 < CaptHindsight> most don't look active 13:22 < CaptHindsight> just a webpage, facebook etc 13:23 < juri_> streety: do i know you? ;) 13:24 < streety> I'm not sure 13:24 < streety> this is me -> https://github.com/streety/ 13:24 < juri_> have you dropped by hacDC for a 3d thursdays? 13:25 < streety> no, not yet 13:25 < streety> I keep meaning to make the trip, but other things keep cropping up 13:26 < kanzure> CaptHindsight: yes, generally biologists are quite isolated from the engineering and computation people 13:26 < juri_> ah. i'm the instructor there, and was a member of the meat hacking group. 13:26 < kanzure> additionally, many of the community labs tend to take most of their activity in person instead of online, thus why there's no content (sadly) 13:27 < kanzure> CaptHindsight: yes, we should talk to the suppliers about sureseal and see what they say 13:29 < kanzure> also might be a good question to post to the diybio mailing list 13:31 < CaptHindsight> maybe write a biologist friendly front end for Linuxcnc 13:31 < CaptHindsight> seems what they might be up to at opentrons 13:32 < CaptHindsight> turn lab work into G-code 13:47 -!- delinquentme [~delinquen@99-8-186-132.lightspeed.snfcca.sbcglobal.net] has quit [Ping timeout: 246 seconds] 14:14 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-rnmhrqhdgxxuddwy] has joined ##hplusroadmap 14:23 < kanzure> "speedup intersection code" https://github.com/pboyer/verb/commit/1a3853e2c1beb6b5f81547ff3378a41080adcf9c 14:28 < kanzure> "A practical algorithm for surface-surface intersection" http://www.cs.berkeley.edu/~hling/research/paper/intersection.htm 14:34 -!- CyberEater [~Genesteal@c-69-255-209-151.hsd1.md.comcast.net] has joined ##hplusroadmap 14:44 -!- _0bitcount [~Big_Byte@85.251.242.3.dyn.user.ono.com] has left ##hplusroadmap ["Leaving"] 14:53 -!- Beatzebub [~beatzebub@d162-156-106-225.bchsia.telus.net] has joined ##hplusroadmap 14:56 < juri_> CaptHindsight: I maintain a 3d modelling system that spits out 2D gcode for laser cutting. 15:04 < CaptHindsight> juri_: I've built all sorts of lab robots using Linuxcnc for the controller 15:05 < CaptHindsight> what is missing is an easy to use G-code generator to create programs 15:06 < CaptHindsight> program routines where it's just pick-n-place or dispense from a nozzle or syringe 15:08 < kanzure> have you seen the octoprint tool? 15:08 < CaptHindsight> or a gantry that moves from spot to spot raising and lowering something to set locations and intervals 15:09 < kanzure> this has a simple web interface for planning 3d prints http://octoprint.org/ 15:09 < CaptHindsight> kanzure: I've only seen the website. I didn't see any tools 15:09 < kanzure> there's some screenshots here https://www.youtube.com/watch?v=TQzGQN440Ns 15:09 < CaptHindsight> oh .org vs .com 15:10 < kanzure> around 1m 40sec i guess 15:11 < CaptHindsight> oh great, they went weenie and built if for repraps 15:11 < kanzure> yeah... 15:11 < CaptHindsight> toys vs machines 15:15 < CaptHindsight> I use this a great example of a horrible UI for multi axis machines http://reprap.org/mediawiki/images/1/12/Pronterface.png 15:16 -!- Beatzebub_ [~beatzebub@d162-156-106-225.bchsia.telus.net] has joined ##hplusroadmap 15:16 < CaptHindsight> https://fedoraproject.org/w/uploads/4/4b/RepetierHost.png Repetier knew what to do 15:18 -!- Beatzebub_ [~beatzebub@d162-156-106-225.bchsia.telus.net] has quit [Client Quit] 15:19 -!- Beatzebub [~beatzebub@d162-156-106-225.bchsia.telus.net] has quit [Ping timeout: 264 seconds] 15:26 -!- FourFire [~FourFire@82.146.86.195] has quit [Ping timeout: 248 seconds] 15:48 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-sexmuyfeosqkmbla] has quit [Quit: Connection closed for inactivity] 15:58 -!- maaku [~quassel@50-0-37-37.dsl.static.fusionbroadband.com] has quit [Ping timeout: 248 seconds] 15:58 -!- maaku [~quassel@50-0-37-37.dsl.static.fusionbroadband.com] has joined ##hplusroadmap 15:59 -!- maaku is now known as Guest34186 16:02 < kanzure> superkuh: mike darwin does something similar to your "constant audio playback of scientific documents" technique. 16:02 -!- Guest34186 [~quassel@50-0-37-37.dsl.static.fusionbroadband.com] has quit [Client Quit] 16:03 -!- maaku_ [~quassel@50-0-37-37.dsl.static.fusionbroadband.com] has joined ##hplusroadmap 16:07 -!- maaku_ [~quassel@50-0-37-37.dsl.static.fusionbroadband.com] has quit [Remote host closed the connection] 16:14 < kanzure> biopreservation inc. documents http://www.cryocare.org/index.cgi?subdir=bpi&url=bpi.html 16:16 -!- panax [~panax@pool-72-91-154-76.tampfl.fios.verizon.net] has joined ##hplusroadmap 16:20 < juri_> I like the pronterface UI. what don't you like about it? 16:22 < kanzure> http://cryoeuro.eu:8080/download/attachments/1441801/Darwin_Michael_+G_+Biography_v4.3.pdf 16:22 < CaptHindsight> juri_: you can't see what the tool is doing 16:22 < kanzure> "As a child, Darwin initially experimented with halting decomposition in small dead animals by 16:22 < kanzure> immersing them in 70% isopropyl alcohol." 16:23 < juri_> it's pretty intuitive to me. 16:24 < CaptHindsight> juri_: for comparison look at Axis, Gmoccapy in Linuxcnc or Repetier 16:24 < juri_> ugh. repetier. non-free #(%$. 16:25 < juri_> haven't looked at the others. 16:36 -!- p4nd4 [~quassel@104.219.54.200] has quit [Quit: No Ping reply in 180 seconds.] 16:38 -!- p4nd4 [~quassel@104.219.54.200] has joined ##hplusroadmap 16:46 < streety> I've observed someone using an interface similar to the Pronterface UI, even with regular use they still needed to stop and think about which direction they wanted to move . . . every time they wanted to move 16:47 < kanzure> eh just use a joystick 16:48 < kanzure> wait do they still make joysticks anymore 16:55 < streety> not sure that solves the issue. you could still match up movements either looking down from above or up from beneath, etc 16:59 < kanzure> well, it makes sense to think before moving a machine, perhaps a simulation mode would be useful 17:17 < streety> To a certain extent you are correct. I would suggest thinking about the movement you want to make is sensible, but being forced to think about how to execute a movement is just bad design 17:43 -!- delinquentme [~delinquen@c-69-181-189-9.hsd1.ca.comcast.net] has joined ##hplusroadmap 18:10 < CaptHindsight> https://www.youtube.com/watch?v=4Mz7tzVSsYk here the AXIS GUI in Linuxcnc you can preflight the program and also watch the position in real time 18:16 < CaptHindsight> https://www.youtube.com/watch?v=fWKYQUj5AOs or using the SIM 18:19 < kanzure> he is suggesting that i sleep in DMSO i am not sure i trust him 18:22 < CaptHindsight> the DNA inkjet can simulate it's program before execution to see if you programmed something goofy 18:23 < CaptHindsight> the worst thing is crash the head into something 18:24 < CaptHindsight> we sometimes add a laser to set a ZERO for Z so you don't crash the head while scanning 18:40 -!- signo5 [~signo5@unaffiliated/signo5] has joined ##hplusroadmap 18:41 < kanzure> brian wowk made some molecules that act like antifreeze proteins except better 18:42 < kanzure> tree frogs can survive freezing up to -20 celsius 18:43 < kanzure> mammalian brains can survive being 60% frozen, no memory loss 18:46 < kanzure> the tree frogs make a cryoprotectant with glucose. then they move all their water from their organs to various body cavities, like between their lungs or their cerebrospinal fluid cavities. 18:47 < kanzure> he has frozen his abdomen before.... 18:49 < kanzure> apparently the way to survive frostbite is to not bend the appendage at all, and warming it up in a sane way. otherwise you irreversibly damage it. 18:51 < drethelin> good to know 19:00 < kanzure> also he says you need to use microwave/rf reheating to avoid ice nucleation, which is a problem fahy has run into 19:00 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-rnmhrqhdgxxuddwy] has quit [Quit: Connection closed for inactivity] 19:03 < kanzure> he was using fluorocarbon to perfuse dogs after vitrifying and after perfusing with cryoprotectant, to get dogs down 5 degrees celsius/minute (this is much faster than normal) 19:04 < kanzure> "you can perfuse a person at 1000 liters/minute with gas" 19:06 < kanzure> "use a hyperbaric chamber to increase the heat transfer capacity, and move 10x more the heat with the same volume of gas" (well duh?) 19:07 < kanzure> "the human body will tolerate 500 psi just fine, but not 2000 psi" 19:09 < drethelin> wouldn't want to kersplode 19:11 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 264 seconds] 19:34 -!- FourFire [~FourFire@82.146.86.195] has joined ##hplusroadmap 19:47 -!- proofoflogic [uid65184@gateway/web/irccloud.com/x-rfxfayubzuxtmoou] has quit [Quit: Connection closed for inactivity] 19:55 < CaptHindsight> ooh, I can get Epson 1430 printheads from China in bulk 19:58 -!- delinquentme [~delinquen@c-69-181-189-9.hsd1.ca.comcast.net] has quit [Ping timeout: 244 seconds] 20:04 < kanzure> hmm why aren't we using gas sterilization instead of autoclaves 20:15 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap 20:18 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 20:18 -!- crescendo [~mozart@unaffiliated/crescendo] has quit [Ping timeout: 256 seconds] 20:18 -!- crescendo [~mozart@unaffiliated/crescendo] has joined ##hplusroadmap 20:21 < kanzure> intranasal insulin apparently helps with alzheimers 20:25 < drethelin> aren't autoclaves more general 20:27 < kanzure> something about a japanese anime about racoons... and then japan started to import racoons? wtf 20:28 < drethelin> japan already had raccoon dogs 20:33 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-urqaezmxuotwzjnv] has joined ##hplusroadmap 20:33 -!- AmbulatoryCortex [~Ambulator@173-31-9-188.client.mchsi.com] has joined ##hplusroadmap 20:35 -!- FourFire [~FourFire@82.146.86.195] has quit [Ping timeout: 255 seconds] 20:37 < kanzure> "biological effects of freezing and super cooling" 20:37 < kanzure> "the shallows" (meh) 20:42 < kanzure> he works at a gas station 20:51 -!- Porb [~Porbus@CPE-124-180-167-154.lns6.lon.bigpond.net.au] has joined ##hplusroadmap 21:01 < CaptHindsight> kanzure: sent updated specs, I'll finish it tomorrow 21:05 < kanzure> awesome; i'm still on the phone but will look. 21:07 < kanzure> yes i think we need to focus on the interface with the chemicals eventually, but this is an excellent start to the electromechanical components 21:28 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Quit: reboot] 21:31 -!- Acty [uid89656@gateway/web/irccloud.com/x-ebhknhxflpoktmeo] has quit [Quit: Connection closed for inactivity] 21:34 -!- ebowden [~ebowden@101.177.244.210] has joined ##hplusroadmap 21:53 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap 21:55 -!- Porb [~Porbus@CPE-124-180-167-154.lns6.lon.bigpond.net.au] has quit [Ping timeout: 256 seconds] 22:19 < kanzure> .title https://www.youtube.com/watch?v=Fjr3A_kfspM 22:19 < yoleaux> Freezing North American Wood Frogs - YouTube 22:22 < kanzure> .wik rana sylvatica 22:22 < yoleaux> "The wood frog (Lithobates sylvaticus) has a broad distribution over North America, extending from the southern Appalachians to the boreal forest with several notable disjunct populations including lowland eastern North Carolina." — https://en.wikipedia.org/wiki/Rana_sylvatica 22:24 < kanzure> "... wood frogs can tolerate the freezing of their blood and other tissues.[6][7] Urea is accumulated in tissues in preparation for overwintering, and liver glycogen is converted in large quantities to glucose in response to internal ice formation. Both urea and glucose act as cryoprotectants to limit the amount of ice that forms and to reduce osmotic shrinkage of cells.[8][9] Frogs can survive many freeze/thaw events during winter if no ... 22:24 < kanzure> ... more than about 65% of the total body water freezes." 22:25 < kanzure> "Biochemical adaption for freezing tolerance in the wood frog,Rana sylvatica" http://link.springer.com/article/10.1007/BF00688788 22:25 < kanzure> "Hatchling turtles survive freezing during winter hibernation" http://www.pnas.org/content/85/21/8350.full.pdf 22:26 < kanzure> "Hypometabolic homeostasis in overwintering aquatic amphibians" http://jeb.biologists.org/content/200/2/387.full.pdf 22:26 < kanzure> "Strategies for exploration of freeze responsive gene expression: advances in vertebrate freeze tolerance" http://www.researchgate.net/profile/Kenneth_Storey/publication/8609790_Strategies_for_exploration_of_freeze_responsive_gene_expression_advances_in_vertebrate_freeze_tolerance/links/0deec51f7e22fbe53d000000.pdf 22:26 < kanzure> "Glucose concentration regulates freeze tolerance in the wood frog Rana sylvatica" http://jeb.biologists.org/content/181/1/245.full.pdf 22:27 < kanzure> "Cold ischemic organ preservation: lessons from natural systems" http://www.researchgate.net/profile/Kenneth_Storey/publication/8174973_Cold_ischemic_organ_preservation_Lessons_from_natural_systems/links/0deec51f7e23188749000000.pdf 22:28 < kanzure> "Forever young: mechanisms of natural anoxia tolerance and potential links to longevity" http://downloads.hindawi.com/journals/omcl/2010/306209.pdf 22:31 < kanzure> "Genetic and epigenetic regulation in hypometabolism" http://trnres.com/ebook/uploads/nowakowska/T_13334498848%20Nowakowska.pdf 22:32 < kanzure> ah primate torpor is apparently a thing, http://www.sciencedirect.com/science/article/pii/S1672022915000467 22:32 < kanzure> "Primate Torpor: Regulation of Stress-activated Protein Kinases During Daily Torpor in the Gray Mouse Lemur, Microcebus murinus" 22:33 < kanzure> "Very few selected species of primates are known to be capable of entering torpor. This exciting discovery means that the ability to enter a natural state of dormancy is an ancestral trait among primates and, in phylogenetic terms, is very close to the human lineage. To explore the regulatory mechanisms that underlie primate torpor, we analyzed signal transduction cascades to discover those involved in coordinating tissue responses ... 22:33 < kanzure> ... during torpor. The responses of mitogen-activated protein kinase (MAPK) family members to primate torpor were compared in six organs of control (aroused) versus torpid gray mouse lemurs, Microcebus murinus. The proteins examined include extracellular signal-regulated kinases (ERKs), c-jun NH2-terminal kinases (JNKs), MAPK kinase (MEK), and p38, in addition to stress-related proteins p53 and heat shock protein 27 (HSP27)." 22:33 < kanzure> "Importantly, both adipose tissues possess specialized functions that are critical for torpor, with brown adipose required for non-shivering thermogenesis and white adipose utilized as the primary source of lipid fuel for torpor. Overall, these data indicate crucial roles of MAPKs in the regulation of primate organs during torpor." 22:35 < kanzure> an interesting variation (that mike did not suggest..) is that you could do directed evolution and selective breeding for organs that better survive freezing, and in particular you could select the ones that seem to make gross anatomical changes during the freezing process (or in preparation for freezing- perhaps with some signaling method that you pick). these changes might not confer any survival benefit whatsoever, except that we ... 22:35 < kanzure> ... suspect that the changes might confer survival benefits. so we can just select the organs that seem to be more likely to survive, even if they aren't actually surviving yet. 22:35 < kanzure> (however, since organs seem to already be surviving at some rate, it seems like that sort of technique will not be required) 22:39 < kanzure> and for the purposes of recovery, you could do the reverse- something like, take an organ down to low temperature but not freezing, and then check whether the changes you want are happening, and select the organisms for the ones that perform appropriate cleanup and resuscitation, and uhh then figure out some way to ensure that those genetic expression networks are triggered when thawing 4realz 22:49 -!- AmbulatoryCortex [~Ambulator@173-31-9-188.client.mchsi.com] has quit [Read error: Connection reset by peer] 22:50 < drethelin> hmm 22:50 < drethelin> I'm confused by what the goal is here 22:50 < drethelin> to figure out how to adapt humans to cryogenics? 22:50 < drethelin> also torpor is really interesting, especially hibernation 22:51 -!- vivi [~vivi@inhu.me] has quit [Read error: Connection reset by peer] 22:51 < drethelin> if we can figure out how it works we can cure something like obesity like THAT 22:51 < drethelin> with few if any long-term side efefcts 22:51 -!- vivi [~vivi@inhu.me] has joined ##hplusroadmap 22:51 < drethelin> if we can duplicate the fat deposition and burning switches 22:51 -!- vivi is now known as Guest92408 23:21 -!- Quashie_ [~boingredd@50.14.92.17] has quit [Ping timeout: 255 seconds] 23:26 < kanzure> drethelin: i just want adaptation of any animal to cryonics (including cryoresuscitation), not necessarily human yet 23:26 < kanzure> thus my selective breeding and directed evolution project description from earlier this year 23:27 < drethelin> ah 23:47 -!- Porb [~Porbus@CPE-124-180-167-154.lns6.lon.bigpond.net.au] has joined ##hplusroadmap --- Log closed Sun Jul 12 00:00:08 2015