--- Log opened Sat Jul 30 00:00:11 2016
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01:28 < ebowden_> kanzure, what happened to paperbot?
01:28 < ebowden_> Was it left useless by the takedown of libgen?
01:38 < archels> it's redundant now, just use sci-hub
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02:47 < ebowden_> I seem to get directed to something of the same name that has a paywall.
02:48 < ebowden_> http://sci-hub.cc
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06:35 < kanzure> ebowden_: there are many different scihub urls
06:36 < ebowden_> Good idea.
06:36 < ebowden_> God knows how many powerful people want them to shut down.
06:39 < chris_99> isn't there a tor hidden service
06:39 < chris_99> too
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07:08 < JayDugger> Wow, laser projectors have gotten a whole lot cheaper since consumer electronics companies took over from aerospace companies. As in two orders of magnitude cheaper, although you don't have the near-IR in home units. So they won't work with NVGs.
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07:10 < JayDugger> "Ridiculous that this isn't available to everybody." Yeah, perhaps you should cut back for a little while.
07:11 < chris_99> how much are they now, also how do they tend to work, similar to a DLP projector?
07:11 < chris_99> with a MEMS chip?
07:12 < JayDugger> Down from over USD1000000 to about USD10000 or less. Overview here: http://www.cnet.com/news/why-lasers-are-the-future-of-projectors/
07:13 < chris_99> aren't there really cheapo ones, i thought you can get a pocket one for <1k
07:13 < JayDugger> Looks like it, from the CNET article. I'd have to look to see whether these match the ones from Raytheon, Evans & Sutherland, or Rheinmetall.
07:14 < chris_99> am i right in thinking with a laser projector, you could potentially make a decent HUD
07:16 < JayDugger> Ah, not these as is, no. Think cinema or very large television in a fixed installation.
07:17 < chris_99> why not out of interest, i mean the small pocket ones
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07:20 < chris_99> ooh http://www.sony.com/electronics/portable-projectors/mp-cl1
07:20 < chris_99> that's rather tempting
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07:39 < CaptHindsight> Casio  started selling some decently powered ones a few years ago https://www.youtube.com/watch?v=gF4HG6v29UY
07:41 < CaptHindsight> ~$600 used on fleabay
07:41 < chris_99> ooh
07:41 < chris_99> i will have a looky
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07:41 < chris_99> how long would a laser diode last for do you know
07:42 < CaptHindsight> http://www.ebay.com/itm/like/331815194881
07:42 < chris_99> cool
07:42 < CaptHindsight> 10k's of hours if kept cool enough
07:43 < chris_99> appaerntly that isn't purely a laser
07:43 < chris_99> http://www.casio.com/products/archive/Projectors/Slim_Projectors/XJ-A140
07:44 < CaptHindsight> ah the Google changed the search on me
07:44 < CaptHindsight> how nice of them
07:44 < CaptHindsight> you type in one part number and they return results for another
07:44 < chris_99> heh
07:44 < chris_99> whats the part no. of the one you were looking for
07:45 < CaptHindsight> it's in the video
07:45 < CaptHindsight> Casio A140 projector
07:46 < chris_99> i think that is the same as the one i linked to, but it also somehow uses an led somehow too?
07:46 < CaptHindsight> I think they stopped selling the high powered laser version after all the hacking
07:46 < chris_99> ah
07:46 < CaptHindsight> so look for used
07:46 < CaptHindsight> some people ruin everything
07:47 < chris_99> http://www.ebay.co.uk/itm/REDUCED-Casio-XJ-A140V-DLP-LED-Projector-HDMI-VGA-2500-Lumens-/371692918939?hash=item568a9fd89b:g:2OEAAOSwARZXlfZU
07:47 < chris_99> could that be it
07:47 < CaptHindsight> https://www.youtube.com/watch?v=ryLEMobdicw
07:47 < chris_99> that's very tempting heh, for ~£100
07:48 < chris_99> what's happening in that video? i'm confused how the beams converge
07:48 < chris_99> then disperse
07:53 < CaptHindsight> he steered them all to ~1ft in front of the lens
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07:55 < chris_99> ah
07:56 < CaptHindsight> looks like the newer Casio SLIM XJ-A142 has the lasers
07:57 < CaptHindsight> http://www.ebay.com/itm/BRAND-New-Casio-SLIM-XJ-A142-XGA-Laser-LED-Projector-2500-Lumens-Factory-Sealed-/152009792183
07:59 < CaptHindsight> maybe all the slim versions are laser since there isn't room for a lamp
08:00 < chris_99> mm, apparently the 140 one is 1024x768, but still seems a pretty good deal used
08:01 < CaptHindsight> how will you use it?
08:01 < CaptHindsight> burn stuff real good?
08:01 < chris_99> heh, just for movies
08:03 < CaptHindsight> Casio XJ-M141 also appears to be lamp free
08:05 < CaptHindsight> the casio site does a good job of listing the lamp free models
08:06 < CaptHindsight> http://www.casio.com/products/Projectors/
08:06 < chris_99> cool, i'll have a look
08:11 < kanzure> greetings from california
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08:15 < CaptHindsight> I recall reading about how the output rating of the projects has stayed the same but they use much lower powered laser leds now
08:15 < CaptHindsight> so you still might want to find an older model that you can drive hard but add some extra cooling
08:16 < chris_99> ah interesting
08:16 < kanzure> CaptHindsight: tell me things that are up
08:17 < CaptHindsight> kanzure: just heading out to mix chems, be back much later
08:20 < CaptHindsight> http://www.laserpointersafety.com/news/news/other-news_files/tag-casio.php#on
08:20 < CaptHindsight> https://www.youtube.com/watch?v=IzUoe-9bKa0
08:20 < chris_99> haha
08:20 < chris_99> that looks safe
08:20 < CaptHindsight> Homebuilt 200W LASER BAZOOKA!!!!!
08:21 < chris_99> hehe
08:21 < CaptHindsight> http://www.laserpointersafety.com/news/news/other-news_files/41601c4111261e7f9c6dcc086a06f9c8-186.php#on
08:21 < CaptHindsight> Germany: "Iron Man" laser fires Class 4 beam from a hobbyist's palm
08:22 < chris_99> heh
08:22 < CaptHindsight> at least no Yodas
08:26 < kanzure> CaptHindsight: show me interesting photoresins
08:37 < CaptHindsight> http://www.pnas.org/content/101/15/5488.full.pdf  Photocleavable fluorescent nucleotides for DNA
08:37 < CaptHindsight> http://www.genelink.com/newsite/products/mod_detail.asp?modid=133
08:38 < kanzure> yep
08:38 < CaptHindsight> they have a bunch of PC stuff
08:38 < kanzure> i think you should be looking at amino acids that are photoresponsive
08:38 < CaptHindsight> or make your own
08:38 < kanzure> anything about amino acid chemistry and photo-anything
08:38 < CaptHindsight> bbl
08:38 < kanzure> photoresponsive nucleotides are useful too and it's definitely going into that "electronically-directed polymerase" paper
08:38 < kanzure> but amino acids that inflate to 10x their size upon illumination (or something) would be particularly useful
08:39 < CaptHindsight> a team of useful people would also be useful
08:39 < CaptHindsight> but where did they all go?
08:40 < kanzure> gotta pay 'em
08:41 < CaptHindsight> I'd have to dig them up
08:41 < kanzure> graverobbing this early in the morning? unlikely
08:59 < kanzure> re: an in vitro selection method for electronically-directed polymerase; an interesting first step would be CMOS array with polymerases acting as resistors (or other components, w/e) strung up between two electrodes, maybe a million or more per chip, and then merely testing the ability for different amino acids to allow for optical illumination to change electrical characteristics of the circuit as discriminated by sending signals down ...
08:59 < kanzure> ... the device to each array element
08:59 < kanzure> and this would be without nucleotide incorporation, without DNA synthesis activity, etc.
09:01 < kanzure> really it needs to be a chip inside of an emulsion drop that could be remotely powered, so that you could have 10^12 emulsion drops and each one with different genotype-phenotype linkage and different mutant polymerase connected to the two antibody-conjugated electrodes. and then some sort of remote interrogation of this device, with optics and power transmission. then you pump a few thousand billion of these past a fluorescent emulsion ...
09:01 < kanzure> ... drop sorting apparatus, which would be responsible for determining whether an emulsion compartment should be selected or not.
09:02 < kanzure> the reason that compartments are necessary here is to separate genotype-phenotype from all the other samples, if there's a way to do that on a giant CMOS array without individual emulsion drops then that's fine and we should pursue that, although i don't know how to do it
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09:20 < JayDugger> Those projectors I mentioned advertise lifetimes of about 20000 hours.
09:21 < chris_99> yeah so do the casio ones
09:22 < chris_99> i'm quite tempted by the cheapo 1024*768 one
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09:43 < CaptHindsight> don't they teach you guys anything in EE school?
09:43 < CaptHindsight> keep the led's cooler, then they will last longer
09:44 < CaptHindsight> you don't really expect a consumer oem to make something that lasts for years
09:44 < CaptHindsight> look up some articles on MTBF vs operating temperature
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09:52 < chris_99> could they not use some form of peltier
09:52 < chris_99> cooling
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11:11 < streety> or just replace in 2 years
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12:48 < kanzure> you could probably use surface-immobilized polymerase on a CMOS chip, then use surface patterning to make it so that emulsion droplets could get stuck to each region, perhaps using either liquid filling a container, or through surface patterning like the hydrophobic grating stuff.   use inkjet printing to deposit the different materials into each emulsion droplet. have the underlying circuits capable of physical addressing.   link ...
12:48 < kanzure> ... genotype-phenotype inside each compartment, for whatever mutant polymerase is used inside that compartment. bottom of the compartment is the interface with the chip.  individual genes can be recovered from this setup by using micropipetting to the specified (x,y) coordinates.
12:50 < kanzure> if they are physically rescessed wells then there is probably no reason to bother with emulsion containment, as long as you do not have liquid spillover from one well to another
12:50 < kanzure> rescinded wells? rescessed? whatever.
12:51 < kanzure> interestingly, once you have that basic chip working, you can reuse it for most of those polymerase evolution projects because the basic way of conjugating a polymrase to a circuit doesn't require any changes - it's the polymerase that is being changed.
13:02 < archels> "Introduction to Brains as Emergent Turing Machines"
13:02 < archels> this ought'a be good
13:05 < archels> the near- and long-term applications slides in the back are empty
13:06 < archels> (there are 83 slides in total)
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13:53 < kanzure> the inkjetting requirement above is merely that mutant polymerases (and their genes) get distributed over the surface of a chip, and that the gene and polymerase both get into the same droplet, but it doesn't matter where on the surface they go. the other requirement is that each location with the polymerase hooked up to the circuit needs to be physically addressable by a pipetting machine, for selecting that element for PCR. it's okay ...
13:53 < kanzure> ... to have polymerases that don't bind to a junction, and it's okay to have emulsion drops that don't get used but it's important that only one emulsion drop lands on a region on the chip (and not two or more emulsion compartments) because that causes genetic mixing.
13:54 < kanzure> and i think an inkjet printhead could be made to deposit emulsion drops like that on to a surface.
13:55 < kanzure> perhaps even with the energy from impact, the drops still wouldn't merge together? or perhaps there's an oil-in-water emulsion that will prevent compartments from merging and mixing in that scenario?
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14:39 < CaptHindsight> make drops from an inkjet stop, move and stop on a tray
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22:06 -!- justanotheruser is now known as KilometersDavis
22:20 < kanzure> eriqoejriqejiadfiadfqioieqr
22:26 < KilometersDavis> I see
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22:33 < kanzure> yea it's a problem
22:37 < nmz787> sup
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22:50 < kanzure> hanging out in california
22:51 < kanzure> nmz787: https://twitter.com/kanzure/status/759560626498777088
22:51 < nmz787> hmm, I think I drove 130 miles today
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23:24 < maaku> That's 150 words per mile. Keep it up.
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--- Log closed Sun Jul 31 00:00:12 2016