--- Log opened Thu Nov 03 00:00:39 2016
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01:51 < archels_> .title http://www.cell.com/cell/abstract/S0092-8674(13)00770-8
01:51 < yoleaux> archels_: Sorry, that doesn't appear to be an HTML page.
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03:54 < kanzure> adlai: i must have lobotomied that particular cortex, i don't seem to have that
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06:13 < JayDugger1> I have a much less tasteful joke about Baby Boomers.
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06:22 < wrldpc1> I love less tasteful jokes about baby boomers.
06:22 < wrldpc1> Old Economy Steve is hilarious but grossly inadequate.
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11:55 < nmz787_> .wik expansion microscopy
11:55 < yoleaux> "Expansion Microscopy (ExM) is a sample preparation tool for biological samples that allows investigators to identify small structures by expanding them using a polymer system." — https://en.wikipedia.org/wiki/Expansion_microscopy
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11:56 < nmz787_> uses baby diaper chemical and hydrogel, embed a sample in the gel, polymerize, then digest sample and expand gel by adding water
11:58 < nmz787_> heh, using DNA oligos as linkers
11:59 < chris_99> nmz787_, you played with fixing the SEM you got, of late, out of interest?
11:59 < nmz787_> chozinksi et al. Nature Methods 2016
12:00 < nmz787_> chris_99: routed some new wires from my power panel in my house last month, need to break the wire ends out in the lab room and install the plugs
12:00 < nmz787_> chris_99: but no, need power first
12:01 < chris_99> ah, does a sem require a fair amount of power out of interest, or do they just run from a normal plug
12:01 < chris_99> i'm assuming the latter
12:05 < chris_99> also in the US, are you allowed to wire up your whole house out of interest, we have a stupid law that prevents that now
12:15 < kanzure> greets
12:17 < nmz787_> it takes 10-15 amps at 100V AC
12:17 < nmz787_> but my office/lab was already on one circuit with a bathroom and utilityroom lights
12:17 < nmz787_> chris_99: laws like this mostly depend on the state, but standards are set by state and fed
12:17 < nmz787_> so in Oregon homeowners can do any works they want, as long as it conforms with code and they get permits (which come with inspections)
12:17 < nmz787_> but not all work needs permits
12:17 < nmz787_> hmm, reverse shrinky-dink for chemical synthesis?
12:18 < chris_99> aha gotcha, is 15 amp via a normal plug then i guess?
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12:27 < nmz787_> well we have 110-120VAC so I have a variac to get it to 100V
12:28 < chris_99> ah interesting
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12:52 < nmz787_> .g biowave pro
12:52 < yoleaux> http://ipv6.google.com/sorry/index?continue=http://www.google.com/search%3F%26q%3Dbiowave%2520pro%26btnI%3D&q=EhAqAQT4AgFyMAAAAAAACGrnGOSq7sAFIhkA8aeDS1qaziOHmVtDHOlsKibXkdLU1dnjMgFj
12:52 < nmz787_> I wonder how well it makes popcorn
12:59 < nmz787_> .wik antigen retrieval
12:59 < yoleaux> "Tissues that have been preserved with formaldehyde, a highly reactive compound, contain a variety of chemical modifications that can reduce the detectability of proteins in biomedical procedures such as immunohistochemistry. Antigen retrieval is an approach to reducing or eliminating these chemical modifications." — https://en.wikipedia.org/wiki/Antigen_retrieval
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13:41 < kanzure> https://gitter.im/Keras-io/Lobby
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14:28 < kanzure> softmax activation stuff https://www.tensorflow.org/versions/r0.11/tutorials/mnist/beginners/index.html
14:28 < kanzure> http://neuralnetworksanddeeplearning.com/chap3.html#softmax
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15:59 < kanzure> blooop
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16:28 < juri_> meep.
16:39 < kanzure> if you're a roadrunner then the irc channel is just a giant painting
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23:06 < nmz787_> http://www.nature.com.sci-hub.cc/nsmb/journal/v22/n5/pdf/nsmb.3014.pdf#
23:06 < nmz787_> PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome
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