--- Log opened Sun Nov 13 00:00:48 2016
00:03 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has joined ##hplusroadmap
00:19 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Read error: Connection reset by peer]
00:21 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
00:25 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has quit [Quit: wrldpc1]
00:27 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has joined ##hplusroadmap
00:35 -!- abetusk [~abe@68.175.143.22] has quit [Ping timeout: 256 seconds]
00:37 -!- Solvaring [~Solvaring@173.44.37.98] has quit [Quit: I'm off to the fair!]
01:10 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-hgujsklsaxdjbqot] has joined ##hplusroadmap
01:23 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
01:50 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has quit [Quit: wrldpc1]
01:54 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has joined ##hplusroadmap
02:05 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has quit [Quit: wrldpc1]
02:17 -!- esmerelda [~andares@unaffiliated/jacco] has quit [Ping timeout: 240 seconds]
02:18 -!- abetusk [~abe@68.175.143.22] has joined ##hplusroadmap
02:18 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 265 seconds]
02:18 -!- abetusk is now known as Guest22715
02:22 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap
02:24 -!- WizJin [WizJin@gateway/shell/fnordserver.eu/x-rmjmgirfjznxgecq] has quit [Ping timeout: 256 seconds]
02:24 -!- Qfwfq [Qfwfq@gateway/shell/fnordserver.eu/x-elubpdtalyxdzomf] has quit [Ping timeout: 260 seconds]
02:33 -!- m4l3z [~m4l3z@2a01cb040226f200552214ceb31e2996.ipv6.abo.wanadoo.fr] has joined ##hplusroadmap
02:40 -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:b9cb:c463:c6a:227d] has quit [Read error: Connection reset by peer]
02:53 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has joined ##hplusroadmap
02:54 -!- m4l3z [~m4l3z@2a01cb040226f200552214ceb31e2996.ipv6.abo.wanadoo.fr] has quit [Ping timeout: 260 seconds]
03:09 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has quit [Quit: wrldpc1]
03:13 -!- m4l3z [~m4l3z@2a01cb040226f2004c1dfe2dfd51c761.ipv6.abo.wanadoo.fr] has joined ##hplusroadmap
03:36 -!- Guest22715 is now known as abetusk
03:45 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds]
04:00 -!- m4l3z [~m4l3z@2a01cb040226f2004c1dfe2dfd51c761.ipv6.abo.wanadoo.fr] has quit [Quit: Leaving]
04:00 -!- malez_ [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap
04:17 -!- _hanhart [~hanhart@static.141.80.201.138.clients.your-server.de] has quit [Remote host closed the connection]
04:45 -!- sandeepkr [~sandeepkr@111.235.65.5] has joined ##hplusroadmap
04:49 -!- sandeepkr_ [~sandeepkr@111.235.65.5] has quit [Quit: Leaving]
05:27 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection]
05:28 -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap
05:28 -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host]
05:28 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
05:29 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection]
05:30 -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap
05:30 -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host]
05:30 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
05:32 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection]
05:43 -!- ebowden [~ebowden@2001:8003:103c:ee00:a4e3:3706:f3a4:bdb8] has joined ##hplusroadmap
05:43 -!- ebowden [~ebowden@2001:8003:103c:ee00:a4e3:3706:f3a4:bdb8] has quit [Changing host]
05:43 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
06:11 -!- malez_ [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Quit: Leaving]
06:15 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection]
06:22 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap
06:30 -!- JayDugger [~jwdugger@162.216.46.123] has quit [Remote host closed the connection]
06:42 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 245 seconds]
06:50 -!- Orpheon [~Orpheon@46.140.52.182] has joined ##hplusroadmap
06:57 -!- drewbot [~cinch@ec2-107-22-2-119.compute-1.amazonaws.com] has quit [Remote host closed the connection]
06:57 -!- drewbot [~cinch@ec2-54-160-227-34.compute-1.amazonaws.com] has joined ##hplusroadmap
07:03 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
07:20 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 244 seconds]
07:22 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
07:30 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 245 seconds]
07:33 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
07:34 < kanzure> always with you overlord this and overlord that
08:06 -!- Qfwfq [Qfwfq@gateway/shell/fnordserver.eu/x-ajpwxcyokeajctls] has joined ##hplusroadmap
08:07 -!- WizJin [WizJin@gateway/shell/fnordserver.eu/x-ozverxksasrzpytl] has joined ##hplusroadmap
08:22 -!- JayDugger [~jwdugger@47.185.237.246] has joined ##hplusroadmap
08:27 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap
08:55 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 260 seconds]
09:02 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
09:06 -!- Douhet [~Douhet@unaffiliated/douhet] has quit [Ping timeout: 256 seconds]
09:08 -!- Douhet [~Douhet@unaffiliated/douhet] has joined ##hplusroadmap
09:45 -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap
10:08 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 256 seconds]
10:10 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
10:11 < adlai> streety: i don't mean to be a shithead, but doesn't politics belong in ##hminusarchaeology ?
10:13 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Quit: WeeChat 1.5]
10:13 < kanzure> yes.
10:14 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap
10:27 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap
10:54 < kanzure> "Directed evolution relies only on a cycle of introducing diversity into a population followed by the partitioning of that population to isolate the desired function. Theoretically, any population can be systematically optimized towards the desired function by repeating cycles of directed evolution. In practice however, the number of variants in the population can rapidly escalate beyond the sampling capacity of any selection ...
10:55 < kanzure> ... methodology. In addition, as a given functional variant becomes rarer in a population, there is a greater burden on the selection method to isolate them."
10:55 < kanzure> thta's a rather strong claim for directed evolution ("theoretically, any population can be systematically optimized towards the desired function [by directed evolution techniques]"
10:55 < kanzure> )
10:59 -!- up_dn [~up_dn@2601:83:4202:19c8:adb1:abb1:1d43:5b8f] has joined ##hplusroadmap
11:04 -!- jtimon [~quassel@186.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap
11:08 < adlai> my steelman cortex translates "theoretically, " here as "assuming that phenotypes exist to support the target function, and the genotype landscape to reach them isn't too fissured with valleys of unsurvivability, ..."
11:12 -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has joined ##hplusroadmap
11:13 -!- up_dn [~up_dn@2601:83:4202:19c8:adb1:abb1:1d43:5b8f] has quit [Ping timeout: 240 seconds]
11:14 < kanzure> i am not sure how "the gm3 cultivation device" supposedly prevents biofilm formation https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308500/
11:14 < kanzure> adlai: i dunno man, that sounds like a pretty big omission to me
11:14 < adlai> they're lucky we're not their peer review! or maybe irc is the real peer review.
11:15 < kanzure> "In one of the earliest examples of eukaryotic continuous evolution, the Evolugator, a proprietary continuous culturing device that reduces biofilm formation and wall growth (Figure 4), was used to evolve thermotolerant filamentous fungi. During long-term continuous culturing of filamentous fungi, wall growth can be problematic as fungal cells rapidly aggregate, leading to non-uniform culture dilution. To circumvent this issue, de ...
11:15 < kanzure> ... Crécy-Lagard and coworkers devised an alternative method for continuous fungi culturing that dramatically ameliorates fungal wall growth [36,37]. Using this platform over the course of four months, the researchers evolved two thermotolerant variants of the entomopathogenic fungus Metarhizium anisopliae to facilitate the widespread adoption of this fungus for pest control [37]. While successful, this method for continuous directed ...
11:16 < kanzure> ... evolution requires specialized equipment that cannot be readily generalized to alternative selection methodologies."
11:17 < kanzure> "... avoid host strain mutagenesis. A similar approach was recently developed by Chang Liu and coworkers using a system that is completely contained in yeast. The authors used an autonomous DNA replication system from Kluveromyces lactis to enable the replication of a gene of interest in the S. cerevisiae cytosol [39]. Due to the strict requirements for the orthogonal K. lactis DNA polymerase initiation, no crosstalk was observed between ...
11:17 < kanzure> ... the K. lactis components and the native host machinery. To make this system more amenable to directed evolution, the authors used a homology-guided approach to create error-prone DNA polymerase variants that would only replicate the orthogonal plasmid. The optimized system was able to induce ~300× higher rates of mutagenesis in the orthogonal plasmid as compared to the background S. cerevisiae mutagenesis rate, with no reduction in ...
11:17 < kanzure> ... the genomic replication fidelity."
11:36 < kanzure> yashgaroth: what is the current status of one pot dna assembly from thousands of fragments with matching overlaps?
11:36 < kanzure> er, where only two molecules in the pot have matching overlaps, i mean.
11:37 < kanzure> i guess i mean golden gate.
11:45 < kanzure> i think it should be possible to mutate and select for an enzyme that is increasingly good at dna ligation based on long 20mer overlap barcodes with very high specificity.   you'd start by looking for an enzyme that does random ligation, then you'd move on to selecting for an enzyme that does not quite random ligation, which you can check with dna sequencing. over time you should be able to get to an enzyme that does high-specificty dna ...
11:46 < kanzure> ... end joining.   so pcr assembly without primers (or polymerase), i guess.
11:47 < kanzure> oh, then you have barcodes that you have to fix. right...
11:49 < kanzure> ok right, the barcodes could just be the end of the molecules without special random nucleotides. no barcodes needed. however, you would have to make sure that none of your sequences are so short that they would be indistinguishable from each other at the ends.
11:54 < yashgaroth> so you're only assembling two of the fragments, but there's a bunch of random other oligos in the reaction? golden gate's mostly obsolete now, and you're limited with type IIs restriction enzymes
11:56 < yashgaroth> plus there's plenty of enzymes you could add to get it more reliable...for every extra reaction you have happening in the same chamber, you get some exponential or log or w/e increase in potential errors
11:57 < yashgaroth> maybe you get one fragment that's got a single-stranded overhang, homologous to a double-stranded end on another molecule, then use some recombinase enzymes to invade the dsDNA and you fill in the gaps from there
11:58 < yashgaroth> that way you're not relying on sticky end basepairing, since the recombinases actively seek the homologous sequence and hold it on the matching strand until your polymerase or ligase comes along
12:00 < yashgaroth> sorry I've had to do a lot of reading on recombinase machinery for work, people aren't using it for assembly, that I know of, but it's certainly plausible
12:01 < yashgaroth> this is sequence non-specific enzymes, not like Cre or whatever, more RecA
12:06 < yashgaroth> wait are you assembling the whole pot together, just end-to-end with unique matching sequences in between? that's a huge pain
12:07 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 260 seconds]
12:07 -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Quit: Leaving]
12:12 < yashgaroth> otherwise you're talking about super-Gibson, no? I do think there's room for proteins that actively seek and match homologous sequences, to actively increase the specificity of that reaction
12:14 < kanzure> err
12:14 < yashgaroth> fill me in before I continue down this tangent
12:14 < kanzure> no no, i just have to switch my brain into biology mode.
12:15 < kanzure> i want one pot with 10000 unique dna sequences (with trillions of molecules or whatever, i don't care). and then i want an enzyme that assembles a long dna molecule by recognizing matching end overlaps.
12:16 < kanzure> you're probably right about recombinases here
12:17 < yashgaroth> so there's phage proteins that form a filament on ssDNA, and then burn ATP to actively scan dsDNA for a matching sequence (maybe they can scan ssDNA, not sure or would need some work) and when they find a match, they invade the dsDNA and hold the ssDNA strand there until e.g. a polymerase comes along and elongates off that primer
12:18 < kanzure> in the imaginary reaction that i am envisioning,   most of the short strands would find their matching strands at some point, and the enzyme would do its work.   and over time, i would expect the reaction to get less efficient because matching giant dna strands is just physically less likely to happen.
12:19 < yashgaroth> well there is that factor, it's a lot easier with short primers floating around vs. carrying a whole huge DNA molecule, brownian motion etc
12:19 < kanzure> the phage provides a primer? or you mean it's a primer + phage ssDNA
12:19 < yashgaroth> phage protein, so normally you have primers that it attaches to and "scans" with
12:20 < yashgaroth> it's huge in isothermal amplification right now, you have a template but you can amplify exponentially at 37C instead of thermocycling
12:20 < yashgaroth> which allows you to have all sorts of extra enzymes that'd be destroyed by 95C to do other stuff like ligate
12:21 < yashgaroth> so the phage recombinase enzymes replace the heat-melting of the template and amplicon
12:21 < kanzure> neat.
12:22 < yashgaroth> so people use it for that right now, mostly PCR-based detection of pathogens or cancer or w/e, but they could provide actual specificity to sticky-end joining; dunno if anyone's looked into it for that but it's not a terrible idea maybe
12:24 < yashgaroth> otherwise w/ traditional Gibson you have a high chance of a few basepairs sticking randomly and the polymerase don't give a fuck and just fills it in, and you get deletions or mismatches in your final product
12:25 < yashgaroth> so far I only know that they scan a dsDNA target to find homology, and yeah it's a lot faster when it's just primer+enzyme floating around vs. a huge DNA fragment, but it might still be workable
12:27 < yashgaroth> it's like e. coli RecA, in the organism it's not scanning and doing amplification, it's there for DNA repair; but we find a use for things
12:28 < yashgaroth> anyway the generic term is 'recombinase polymerase amplification'
12:35 < kanzure> thanks
12:35 < kanzure> that is helpful
12:39 < yashgaroth> would require some modification for this application, but would be nice to help maintain specificity and reduce off-target ligations
12:52 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
12:57 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 256 seconds]
12:57 -!- Solvaring [~Solvaring@184.75.215.34] has joined ##hplusroadmap
13:03 -!- ebowden [~ebowden@2001:8003:103c:ee00:80b9:b140:6888:4d2b] has joined ##hplusroadmap
13:03 -!- ebowden [~ebowden@2001:8003:103c:ee00:80b9:b140:6888:4d2b] has quit [Changing host]
13:03 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
13:05 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
13:08 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds]
13:12 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 265 seconds]
13:18 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
13:41 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 246 seconds]
13:47 -!- WizJin [WizJin@gateway/shell/fnordserver.eu/x-ozverxksasrzpytl] has quit [Ping timeout: 240 seconds]
13:48 -!- Qfwfq [Qfwfq@gateway/shell/fnordserver.eu/x-ajpwxcyokeajctls] has quit [Ping timeout: 240 seconds]
13:49 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
13:53 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-hgujsklsaxdjbqot] has quit [Quit: Connection closed for inactivity]
13:58 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
13:59 -!- ebowden_ [~ebowden@2001:8003:103c:ee00:d0c0:89fb:aa12:4e25] has joined ##hplusroadmap
13:59 -!- ebowden_ [~ebowden@2001:8003:103c:ee00:d0c0:89fb:aa12:4e25] has quit [Changing host]
13:59 -!- ebowden_ [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
14:03 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds]
14:13 -!- ebowden_ [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection]
14:16 -!- ebowden [~ebowden@2001:8003:103c:ee00:9512:fee7:9120:32d0] has joined ##hplusroadmap
14:16 -!- ebowden [~ebowden@2001:8003:103c:ee00:9512:fee7:9120:32d0] has quit [Changing host]
14:16 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
14:19 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 260 seconds]
14:20 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds]
14:23 -!- ebowden [~ebowden@2001:8003:103c:ee00:9512:fee7:9120:32d0] has joined ##hplusroadmap
14:23 -!- ebowden [~ebowden@2001:8003:103c:ee00:9512:fee7:9120:32d0] has quit [Changing host]
14:23 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
14:31 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving]
14:35 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
14:39 -!- jtimon [~quassel@186.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 258 seconds]
14:55 -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has quit [Remote host closed the connection]
14:58 -!- augur [~augur@c-73-46-94-9.hsd1.fl.comcast.net] has joined ##hplusroadmap
15:23 -!- jtimon [~quassel@186.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap
15:37 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap
15:39 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 265 seconds]
15:40 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Quit: hacked by saddam hussein]
15:42 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap
15:42 -!- justan0theruser is now known as justanotheruser
16:03 -!- newbie is now known as anachronick
16:15 -!- newbie [~kvirc@a81-84-40-93.cpe.netcabo.pt] has joined ##hplusroadmap
16:19 -!- anachronick [~kvirc@a81-84-40-93.cpe.netcabo.pt] has quit [Ping timeout: 260 seconds]
16:40 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds]
16:40 -!- ebowden [~ebowden@2001:8003:103c:ee00:9512:fee7:9120:32d0] has joined ##hplusroadmap
16:40 -!- ebowden [~ebowden@2001:8003:103c:ee00:9512:fee7:9120:32d0] has quit [Changing host]
16:40 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
17:08 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection]
17:09 -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap
17:09 -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host]
17:09 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
17:35 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 260 seconds]
17:47 -!- newbie [~kvirc@a81-84-40-93.cpe.netcabo.pt] has quit [Read error: Connection reset by peer]
18:00 -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has joined ##hplusroadmap
18:46 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 245 seconds]
19:00 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap
19:12 < kanzure> hm.
19:19 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 245 seconds]
19:33 -!- jtimon [~quassel@186.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 260 seconds]
19:41 -!- maaku [~quassel@173-228-107-141.dsl.static.fusionbroadband.com] has quit [Ping timeout: 260 seconds]
19:42 -!- maaku [~quassel@173-228-107-141.dsl.static.fusionbroadband.com] has joined ##hplusroadmap
19:55 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap
20:14 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 245 seconds]
20:18 -!- newbie|2 [~kvirc@a81-84-40-93.cpe.netcabo.pt] has joined ##hplusroadmap
20:19 -!- Joshchamp [sid159459@gateway/web/irccloud.com/x-szhpmpfwhidhrxxg] has left ##hplusroadmap []
20:19 -!- Joshchamp [sid159459@gateway/web/irccloud.com/x-szhpmpfwhidhrxxg] has joined ##hplusroadmap
20:20 < newbie|2> how many percentual decimals of progress towards singularity would you guys say are performed on a single day?
20:20 < newbie|2> and, are these decimals less on weekends?
20:51 -!- Orpheon [~Orpheon@46.140.52.182] has quit [Read error: Connection reset by peer]
20:55 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap
21:14 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 245 seconds]
21:51 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap
22:10 -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 250 seconds]
22:55 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has joined ##hplusroadmap
23:03 -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has quit [Quit: Leaving]
23:23 < vicarion> i would say that percentage is a complex number. possibly lacking a real component many days
23:33 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Read error: Connection reset by peer]
23:34 -!- mf1008 [~mf1008@unaffiliated/mf1008] has quit [Quit: Cave quid dicis, quando, et cui]
23:44 -!- mf1008 [~mf1008@unaffiliated/mf1008] has joined ##hplusroadmap
23:45 -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has quit [Quit: wrldpc1]
23:55 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-oocqokebueprnaco] has joined ##hplusroadmap
--- Log closed Mon Nov 14 00:00:49 2016