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06:13 < kanzure> "When Is the Brain Dead? Living-Like Electrophysiological Responses and Photon Emissions from Applications of Neurotransmitters in Fixed Post-Mortem Human Brains" http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0167231
06:13 < kanzure> "The structure of the post-mortem human brain can be preserved by immersing the organ within a fixative solution. Once the brain is perfused, cellular and histological features are maintained over extended periods of time. However, functions of the human brain are not assumed to be preserved beyond death and subsequent chemical fixation. Here we present a series of experiments which, together,...
06:13 < kanzure> ... refute this assumption. Instead, we suggest that chemical preservation of brain structure results in some retained functional capacity. Patterns similar to the living condition were elicited by chemical and electrical probes within coronal and sagittal sections of human temporal lobe structures that had been maintained in ethanol-formalin-acetic acid. This was inferred by a reliable modulatio...
06:13 < kanzure> ...n of frequency-dependent microvolt fluctuations. These weak microvolt fluctuations were enhanced by receptor-specific agonists and their precursors (i.e., nicotine, 5-HTP, and L-glutamic acid) as well as attenuated by receptor-antagonists (i.e., ketamine). Surface injections of 10 nM nicotine enhanced theta power within the right parahippocampal gyrus without any effect upon the ipsilateral hi...
06:14 < kanzure> ...ppocampus. Glutamate-induced high-frequency power densities within the left parahippocampal gyrus were correlated with increased photon counts over the surface of the tissue. Heschl's gyrus, a transverse convexity on which the primary auditory cortex is tonotopically represented, retained frequency-discrimination capacities in response to sweeps of weak (2?V) square-wave electrical pulses b...
06:14 < kanzure> ...etween 20 Hz and 20 kHz. Together, these results suggest that portions of the post-mortem human brain may retain latent capacities to respond with potential life-like and virtual properties."
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07:40 < kanzure> "Protein damage and death by radiation in Escherichia coli and Deinococcus radiodurans" http://pnas.org/content/107/32/14373.full
07:40 < kanzure> "We show that the extraordinary robustness of D. radiodurans depends on efficient proteome protection (but not DNA protection) against constitutive and radiation-induced PC consisting of low molecular weight cytosolic compounds."
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09:28 <@fenn> wow "when is the brain dead" indeed
09:29 <@fenn> sign me up for formalin fixation
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09:44 < kanzure> probably some other fixation method can preserve even more functionality
09:45 < kanzure> preservation of functionality isn't what's needed, we just need the synapses to be in tact when the whole thing is cut up
09:45 < kanzure> .. or something.
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09:55 < kanzure> proteins could record some information as a last operation, and it's okay if it's toxic because you're already dead
09:59 <@fenn> "because you're already dead" seems to be the issue
09:59 <@fenn> anyway formalin is probably more toxic than any protein you could come up with
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10:15 < kanzure> why are there so many igem projects
10:15 < kanzure> grr
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10:19 < nmz787> kanzure: new idea... we just freeze people's brains, use cryo TEM to get tomography of that snapshot... then we unfreeze and let them go on their way
10:20 < kanzure> i don't think we've counted synaptic weight with TEM yet
10:22 < nmz787> probably skull and brain combo is too thick
10:23 < nmz787> or too thick for today's computers to deconvolve
10:25 < kanzure> removing the skull is fine
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11:47 < kanzure> huh, why doesn't NORAA do santa tracking using radioisotopes
11:48 < cluckj> the NSA does it using surveillance cameras
11:49 < chris_99> heh, how would that work? you mean dump some in nearspace and monitor fluctuations?
11:49 < cluckj> drop some trackable isotopes in his cookies
11:49 < chris_99> hehe
11:49 < chris_99> that's a good plan
11:50 < cluckj> or I guess you could track the isotopes in the anthracite coal he carries to put in bad little kids' stockings?
11:51 < kanzure> "alright kids, this xmas we're going to learn about radiation poisoning"
11:51 < chris_99> heh
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11:53 < cluckj> "you didn't get any presents this year because santa forgot his chelation treatment"
11:53 < kanzure> lightning network stuff https://github.com/lightningnetwork/lightning-rfc
11:55 < kanzure> yea but lying to kids ain't cool
12:03 < kanzure> .title http://www.adapteva.com/uncategorized/epiphany-v-analysis/
12:03 < yoleaux> Epiphany-V Analysis - AI
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12:07 < kanzure> mumble mumble darpa parallela things
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13:21 < Fausta> is anyone here on calorie restriction
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13:30 < kanzure> i forget, i don't track everyone's diets
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14:01 < kanzure> various molecular biology protocols here https://www.neb.com/tools-and-resources/search?type=Protocol&num=1000
14:03 < kanzure> "Paper-based synthetic gene networks" http://www.cell.com/abstract/S0092-8674%2814%2901291-4
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14:10 < kanzure> yashgaroth: what's up with people using dna/rna polymerases that are sensitive to small molecules as a sensor? lots of things can break polymerase..
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14:25 < kanzure> "Dr. George D. Yancopoulos, chief scientific officer of Regeneron, said the crisis had pointed up shortcomings in biodefense. 'Nobody is really prepared,' he said. 'Nobody in the world has rapid response capabilities.'"
14:26 < kanzure> "A cell-free protein producing gel" http://www.nature.com/nmat/journal/v8/n5/abs/nmat2419.html
14:27 < kanzure> "Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system" http://www.sciencedirect.com/science/article/pii/S1389172314001509
14:35 < kanzure> re: algae production of antibodies, "CHO facilities, on average, cost around $200M for 80,000L. A similarly sized algae facility at 15,000L would cost an estimated $1.3M. With economies of scale, algae production facilities become significantly cheaper. $1B would be a 14 Billion Liter algae farm (5,500 acres) according to one techno-economic analysis [10]."
14:35 < kanzure> [10] "A critical analysis of paddlewheel-driven raceway ponds for algal biofuel production at commercial scales" http://www.sciencedirect.com/science/article/pii/S2211926413001008
14:36 < kanzure> "61 of the 90 therapeutic proteins produced in mammalian cells come from CHO's (Rader, 2015. Bioprocessing Journal). Furthermore, of the 174 recombinant FDA approved drugs on the market, only one is made by plant cells (Elelyso from carrot cell culture)."
14:36 < kanzure> from http://2015.igem.org/Team:Northeastern_Boston/Description
14:45 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=0b44537d Bryan Bishop: finish igem 2015 summaries >> http://diyhpl.us/diyhpluswiki/dna/projects/
14:48 < kanzure> .tw https://twitter.com/kanzure/status/810255211168223232
14:48 < yoleaux> One-sentence summaries of most #syntheticbiology projects from @iGEM 2015 http://diyhpl.us/wiki/dna/projects/#igem-2015 (@kanzure)
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16:30 < kanzure> cell-free protein expression system for protein prodution and also testing http://2016.igem.org/Team:BGIC_China/Description
16:31 < kanzure> if cell-free paper-only protein stuff actually works, then yeah i guess it makes sense to use a stack of different papers to filter your liquid
16:31 < kanzure> and then reprogramming becomes a matter of inserting the correct order of filter paper
16:31 < kanzure> oh what about yield problems... how are they handling that.
16:34 < kanzure> Pardee, Keith, et al. "Paper-based synthetic gene networks." Cell 159.4 (2014): 940-954.
16:40 < kanzure> https://github.com/eternitywall/javascript-opentimestamps
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17:28 < kanzure> "Emergent gravity and the dark universe" https://arxiv.org/abs/1611.02269
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--- Log closed Sun Dec 18 00:00:41 2016