--- Log opened Sat Dec 31 00:00:53 2016
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09:22 < kanzure> http://crispr.technology/
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09:24 < kanzure> "Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona" http://biorxiv.org/content/early/2016/12/30/041632
09:24 < kanzure> "... Using high-throughput sequencing of mutagenized alleles, we identified guide sequences that correlate with sgRNA mutagenesis activity and used this information for the rational design of all possible sgRNAs targeting the Ciona transcriptome. We also describe a one-step cloning-free protocol for the assembly of sgRNA expression cassettes. These cassettes can be directly electroporated as u...
09:24 < kanzure> ...npurified PCR products into Ciona embryos for sgRNA expression in vivo, resulting in high frequency of CRISPR/Cas9-mediated mutagenesis in somatic cells of electroporated embryos."
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13:00 < kanzure> "A genome-wide association scan implicates DCHS2, RUNX2, GLI3, PAX1 and EDAR in human facial variation" http://www.nature.com/articles/ncomms11616
13:02 < kanzure> "A genome-wide association scan in admixed Latin Americans identifies loci influencing facial and scalp hair features" http://www.nature.com/articles/ncomms10815
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15:44 < kanzure> "Mechanosensory hair cells express two molecularly distinct mechanotransduction channels" http://www.nature.com/neuro/journal/v20/n1/abs/nn.4449.html
15:44 < kanzure> .wik piezo2
15:44 < yoleaux> "Piezo-type mechanosensitive ion channel component 2 is a protein that in humans is encoded by the PIEZO2 gene." -- https://en.wikipedia.org/wiki/PIEZO2
15:53 < kanzure> "A highly parallel strategy for storage of digital information in living cells" http://biorxiv.org/content/early/2016/12/26/096792
15:53 < kanzure> pfft "As a potential application, an encrypted pathogen genome could be could be distributed and carried by cells without danger of being expressed"
15:58 < kanzure> "Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing" http://www.nature.com/articles/ncomms13268
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16:01 < kanzure> well, to me that sounds like a good alternative to fluoresence in optogenetics
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17:01 < kanzure> "Direct observation of DNA knots using a solid-state nanopore" http://www.nature.com/nnano/journal/v11/n12/full/nnano.2016.153.html
17:05 < kanzure> "DNA condensation in one dimension" http://www.nature.com/nnano/journal/v11/n12/full/nnano.2016.142.html
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19:44 < adlai> that just sounds like a bad idea
19:45 < adlai> someone's going to make like that suicide box that contains its own phage
19:47 < kanzure> phage is friend
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--- Log closed Sun Jan 01 00:00:53 2017