--- Log opened Tue Feb 14 00:00:33 2017 00:03 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Ping timeout: 240 seconds] 00:08 -!- Gurkenglas__ [~Gurkengla@dslb-178-005-218-116.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 264 seconds] 00:25 -!- augur [~augur@2601:645:8300:2b03:adb0:95b0:5b38:4d5a] has joined ##hplusroadmap 00:25 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 00:34 -!- esmerelda [~andares@2607:fb90:8355:35fd:f15:1419:b41d:594b] has joined ##hplusroadmap 00:34 -!- esmerelda [~andares@2607:fb90:8355:35fd:f15:1419:b41d:594b] has quit [Changing host] 00:34 -!- esmerelda [~andares@unaffiliated/jacco] has joined ##hplusroadmap 01:11 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Quit: Leaving.] 01:12 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 01:23 -!- urchin_ [~urchin@89.17.2.88] has quit [Changing host] 01:23 -!- urchin_ [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 01:23 -!- urchin_ is now known as Urchin 01:40 -!- aedla [~quassel@c21-76.uvn.zone.eu] has quit [Read error: Connection reset by peer] 01:41 -!- aedla [~quassel@c21-76.uvn.zone.eu] has joined ##hplusroadmap 01:53 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds] 02:03 -!- ebowden [~ebowden@2001:8003:1174:d800:5131:e4e5:c171:e772] has joined ##hplusroadmap 02:03 -!- ebowden [~ebowden@2001:8003:1174:d800:5131:e4e5:c171:e772] has quit [Changing host] 02:03 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap 02:08 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds] 02:16 -!- pompolic [~A@unaffiliated/pompolic] has quit [Ping timeout: 252 seconds] 02:17 -!- pompolic [~A@unaffiliated/pompolic] has joined ##hplusroadmap 02:25 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-hppelutuwextcaez] has joined ##hplusroadmap 03:03 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 03:03 -!- augur [~augur@2601:645:8300:2b03:adb0:95b0:5b38:4d5a] has quit [Remote host closed the connection] 03:14 -!- ebowden [~ebowden@1.152.96.204] has joined ##hplusroadmap 03:14 -!- ebowden [~ebowden@1.152.96.204] has quit [Changing host] 03:14 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap 03:26 < kanzure> bloop 03:35 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds] 03:36 -!- ebowden [~ebowden@2001:8003:1174:d800:d412:9482:b641:ccb8] has joined ##hplusroadmap 03:36 -!- ebowden [~ebowden@2001:8003:1174:d800:d412:9482:b641:ccb8] has quit [Changing host] 03:36 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap 03:58 -!- bluebear_ [~dluhos@80.95.97.194] has joined ##hplusroadmap 04:10 -!- CRM114 [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 04:46 -!- ebowden_ [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap 04:48 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds] 06:20 -!- jtimon [~quassel@245.30.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 06:35 < kanzure> don't everyone bleep at once 06:42 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-hppelutuwextcaez] has quit [Ping timeout: 240 seconds] 06:43 -!- proofoflogic [sid65184@gateway/web/irccloud.com/x-czxclxwherzhfber] has quit [Ping timeout: 258 seconds] 06:43 -!- strages [sid11297@gateway/web/irccloud.com/x-sulytehbtwjcoeph] has quit [Ping timeout: 258 seconds] 06:44 -!- proofoflogic [sid65184@gateway/web/irccloud.com/x-dgxduaqqxbvcmdps] has joined ##hplusroadmap 06:45 -!- blueskin [~blueskin@unaffiliated/blueskin] has quit [Ping timeout: 258 seconds] 06:46 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-hrvxmzdmbkcapxmd] has joined ##hplusroadmap 06:47 -!- blueskin [~blueskin@unaffiliated/blueskin] has joined ##hplusroadmap 06:47 -!- strages [sid11297@gateway/web/irccloud.com/x-kyvwnwkwfgbndaym] has joined ##hplusroadmap 06:55 < kanzure> "DCAF4, a novel gene associated with leucocyte telomere length" http://jmg.bmj.com/content/early/2015/01/23/jmedgenet-2014-102681.full 06:57 < kanzure> "Mangino et al. (2015) found an association between an intronic G-to-A transition (rs2535913) in the DCAF4 gene and leukocyte telomere length (p = 6.4 x 10(-10)). The minor A allele was associated with shorter LTL (p = 7.8 x 10(-3)). In a second part of the study, the minor A allele was associated with lower expression of DCAF4 in lymphoblastoid cells from 778 twins of European descent, in who... 06:57 < kanzure> ...le blood of 167 individuals, and in sun-exposed skin from 113 individuals; telomere length in these individuals was not reported. Noting that the DCAF4 gene is involved with proteins in the DNA repair pathway and is expressed in sun-exposed skin, Mangino et al. (2015) postulated that the role of DCAF4 in affecting telomere length may suggest a potential role for this gene in the development of... 06:57 < kanzure> ... sporadic melanoma (see 155600)." 06:59 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=1cf9ce31 Bryan Bishop: avoid rs2535913 >> http://diyhpl.us/diyhpluswiki/genetic-modifications/ 07:00 -!- pompolic [~A@unaffiliated/pompolic] has quit [Ping timeout: 240 seconds] 07:08 -!- pompolic [~A@unaffiliated/pompolic] has joined ##hplusroadmap 07:33 -!- Gurkenglas__ [~Gurkengla@dslb-178-005-218-116.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap 07:58 < kanzure> i suppose it's counterproductive to go around listing all the SNPs to avoid, since there's quite a lot that will kill you 08:28 < kanzure> unless it's extremely common? 08:29 < bluebear_> well; some SNPs have a measurable effect after quite a long time, so they can be sneaky 08:30 < bluebear_> I would go after not single SNPs but for whole signaling networks for any kind of human improvement 08:32 < bluebear_> or, if it is infeasible, I'd pick the whole genome from an individual that has the desired trait, and gradually eliminated what does not belong to the trait 08:47 -!- augur [~augur@2601:645:8300:2b03:adb0:95b0:5b38:4d5a] has joined ##hplusroadmap 09:04 < kanzure> http://bellard.org/riscvemu/ 09:08 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 09:46 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Remote host closed the connection] 09:49 -!- augur_ [~augur@2601:645:8300:2b03:79d9:3188:faca:7396] has joined ##hplusroadmap 09:51 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 09:53 -!- augur [~augur@2601:645:8300:2b03:adb0:95b0:5b38:4d5a] has quit [Ping timeout: 255 seconds] 09:54 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-hrvxmzdmbkcapxmd] has quit [Quit: Connection closed for inactivity] 10:25 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 245 seconds] 11:21 -!- bluebear_ [~dluhos@80.95.97.194] has quit [Quit: Leaving.] 11:26 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 11:29 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 11:30 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 245 seconds] 11:51 -!- abetusk [~abe@68.175.143.22] has quit [Remote host closed the connection] 12:16 -!- augur_ [~augur@2601:645:8300:2b03:79d9:3188:faca:7396] has quit [Remote host closed the connection] 12:26 -!- Netsplit *.net <-> *.split quits: ebowden_ 12:26 -!- heimcomputer [~heimcompu@68.72.147.147.dyn.plus.net] has joined ##hplusroadmap 12:31 -!- heimcomputer [~heimcompu@68.72.147.147.dyn.plus.net] has quit [Quit: heimcomputer] 12:49 -!- redlegion [~x@unaffiliated/redlegion] has joined ##hplusroadmap 13:12 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 13:48 -!- esmerelda [~andares@unaffiliated/jacco] has quit [Ping timeout: 255 seconds] 13:48 -!- poppingtonic1 [~brian@197.182.149.167] has joined ##hplusroadmap 13:50 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 13:57 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=fd122795 Bryan Bishop: add some text about evolved microbes as nootropics >> 14:01 -!- abetusk [~abe@68.175.143.22] has joined ##hplusroadmap 14:08 < kanzure> "Muscle-specific CRISPR/Cas9 dystrophin gene editing ameliorates pathophysiology in a mouse model for Duchenne muscular dystrophy" http://www.nature.com/articles/ncomms14454 14:11 < kanzure> "Identification of microsporidia host-exposed proteins reveals a repertoire of rapidly evolving proteins" http://www.nature.com/articles/ncomms14023 14:20 -!- genehacker [~chatzilla@c-50-137-45-249.hsd1.or.comcast.net] has quit [Remote host closed the connection] 14:22 < kanzure> roche/pacbio deal is over, now in favor of roche/genia 14:27 < kanzure> "Induced pluripotent stem cells meet genome engineering" http://www.cell.com/cell-stem-cell/fulltext/S1934-5909%2816%2930056-X 14:27 < kanzure> a review article about iPSCs and cas9 14:29 < kanzure> "Epigenetics of reprogramming to induced pluripotency" http://www.cell.com/cell/fulltext/S0092-8674%2813%2900277-8 14:30 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 14:31 < kanzure> "Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA" http://www.cell.com/cell-stem-cell/fulltext/S1934-5909%2810%2900434-0 14:33 < kanzure> http://www.nature.com/news/us-science-advisers-outline-path-to-genetically-modified-babies-1.21474 14:35 -!- drewbot_ [~cinch@ec2-23-22-29-119.compute-1.amazonaws.com] has joined ##hplusroadmap 14:35 -!- EnabrinTain_ [sid11525@gateway/web/irccloud.com/x-zbijqqdrodcrolkm] has joined ##hplusroadmap 14:38 -!- nmz787_ [~nmz787@unaffiliated/nmz787] has joined ##hplusroadmap 14:39 -!- nickjohnson_ [sid789@gateway/web/irccloud.com/x-ajqnhxlliknggcjo] has joined ##hplusroadmap 14:42 -!- Netsplit *.net <-> *.split quits: sachy, rancyd-, maaku, drewbot, EnabrinTain, nmz787, vikraman, nickjohnson, rancyd 14:44 -!- EnabrinTain_ is now known as EnabrinTain 14:45 -!- maaku [~mark@173.234.25.100] has joined ##hplusroadmap 14:46 -!- Netsplit *.net <-> *.split quits: andytoshi, indiebio, c0rw1n, proofoflogic, Proteus, jrayhawk, CRM114, adlai, Joshchamp 14:46 -!- Netsplit over, joins: andytoshi, jrayhawk 14:46 -!- BobaMa [bobama@kapsi.fi] has quit [Ping timeout: 240 seconds] 14:47 -!- Netsplit over, joins: c0rw1n 14:47 -!- Netsplit over, joins: adlai 14:49 < kanzure> "Retinal-based proton pumping in the near infrared" http://pubs.acs.org/doi/abs/10.1021/jacs.6b11366 14:50 -!- BobaMa [bobama@kapsi.fi] has joined ##hplusroadmap 14:51 -!- sachy [~sachy@nat.brmlab.cz] has joined ##hplusroadmap 14:52 -!- indiebio [~quassel@quassel.tumbleweed.org.za] has joined ##hplusroadmap 14:52 -!- CRM114 [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 14:52 -!- Joshchamp [sid159459@gateway/web/irccloud.com/x-gqkvxczpypzegkfv] has joined ##hplusroadmap 14:52 -!- Proteus [~Proteus@unaffiliated/proteus] has joined ##hplusroadmap 14:53 -!- nickjohnson_ is now known as nickjohnson 14:54 -!- proofoflogic [sid65184@gateway/web/irccloud.com/x-gmcusinevzejhgmc] has joined ##hplusroadmap 15:04 < kanzure> human mutation-increasing mechanisms should probably be disbaled in the genome, if there are any. other people can be the guinnea pigs from now on. 15:04 < kanzure> "In vivo structures of an intact type VI secretion system revealed by electron cryotomography" http://biorxiv.org/content/early/2017/02/14/108233 15:06 < kanzure> diagrams start on pdf page 18 15:11 < kanzure> "Epigenetic editing of Ascl1 gene in neural stem cells by optogenetics" http://www.nature.com/articles/srep42047 15:11 < kanzure> "Enzymes involved in epigenetic processes such as methyltransferases or demethylases are becoming highly utilized for their persistent DNA or histone modifying efficacy. Herein, we have developed an optogenetic toolbox fused to the catalytic domain (CD) of DNA-methyltransferase3A (DNMT3A-CD) or Ten-Eleven Dioxygenase-1 (TET1-CD) for loci-specific alteration of the methylation state at the prom... 15:11 < kanzure> ...oter of Ascl1 (Mash1), a candidate proneuron gene. Optogenetical protein pairs, CRY2 linked to DNMT3A-CD or TET1-CD and CIB1 fused to a Transcription Activator-Like Element (TALE) locating an Ascl1 promoter region, were designed for site specific epigenetic editing. A differentially methylated region at the Ascl1 promoter, isolated from murine dorsal root ganglion (hypermethylated) and striate... 15:11 < kanzure> ...d cells (hypomethylated), was targeted with these optogenetic-epigenetic constructs. Optimized blue-light illumination triggered the co-localization of TALE constructs with DNMT3A-CD or TET1-CD fusion proteins at the targeted site of the Ascl1 promoter. We found that this spatiotemporal association of the fusion proteins selectively alters the methylation state and also regulates gene activity... 15:11 < kanzure> .... This proof of concept developed herein holds immense promise for the ability to regulate gene activity via epigenetic modulation with spatiotemporal precision." 15:11 < kanzure> "Irudayaraj and his team attached these cytosine-modifying enzymes DNMT3A/TET to light-sensitive protein pairs to demonstrate site-specific methylation/demethylation. Zhou and his team introduced those light-sensitive proteins into neural stem cells and found that when they shined a blue light, the methylation modifying enzyme DNMT3A/TET and the gene target came together, adjusting the methyla... 15:11 < kanzure> ...tion of the gene." 15:11 < kanzure> well that's... something. 15:14 < kanzure> that is optogenetic control of methylation 15:15 < kanzure> "By using a passive DNA demethylase (TET1) and DNA methyltransferase (DNMT3A), we showed that DNA methylation could either be increased or decreased at the same sites. With methylation altered by DNMT3A-CRY2-EGFP, we found an average of a 1.58-fold (1.38 to 1.92) increase in DNA methylation across all four CpG sites upon treatment with light, which was associated with a 1.75-fold reduction in ... 15:15 < kanzure> ...Ascl1 gene expression. In contrast, when TET1-CRY2-EGFP was used in the STR cells, an average of 1.1-fold decrease in DNA methylation was observed. Interestingly, although the change in DNA methylation by TET1-CRY2-EGFP is smaller than that by DNMT3A-CRY2-EGFP, we found a greater change in gene expression, which is ~2.6-fold increase, by TET1-CRY2-EGFP." 15:25 < kanzure> so they just stuck some catalytic domains together to a TALE and it worked? 15:30 < kanzure> looks like the same was done with cas9/dCas9.. although the BRCA1 study does not seem to be optogenetic. 15:57 -!- vikraman [~vh4x0r@gentoo/developer/vikraman] has joined ##hplusroadmap 17:15 -!- Gurkenglas__ [~Gurkengla@dslb-178-005-218-116.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 255 seconds] 17:25 -!- urchin_ [~urchin@89.17.8.239] has joined ##hplusroadmap 17:27 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 240 seconds] 17:27 -!- CRM114 [~urchin@unaffiliated/urchin] has quit [Ping timeout: 256 seconds] 17:31 -!- Gurkenglas__ [~Gurkengla@dslb-178-005-218-116.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap 17:37 -!- drewbot_ [~cinch@ec2-23-22-29-119.compute-1.amazonaws.com] has quit [Remote host closed the connection] 17:38 -!- drewbot [~cinch@ec2-54-160-128-145.compute-1.amazonaws.com] has joined ##hplusroadmap 17:41 -!- Gurkenglas__ [~Gurkengla@dslb-178-005-218-116.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 255 seconds] 18:04 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 18:22 -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has joined ##hplusroadmap 18:44 < kanzure> yashgaroth: APOB rs1801703 18:52 < yashgaroth> you got a paper to go with that, all I can find is http://onlinelibrary.wiley.com/doi/10.1111/acel.12261/pdf which says it's not significant for life extension 18:58 * kanzure looks again 18:58 < kanzure> what? 19:07 -!- gnusha_ [~gnusha@unaffiliated/kanzure/bot/gnusha] has joined ##hplusroadmap 19:07 -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | sponsored by lobsters everywhere, banned by the Federal Death Administration (5 times) | this channel is LOGGED: http://gnusha.org/logs | http://diyhpl.us/wiki | "ray kurzweil is a pessimist" - george church 19:07 -!- Topic set by kanzure [~kanzure@unaffiliated/kanzure] [Wed May 20 12:46:25 2015] 19:07 [Users ##hplusroadmap] 19:07 [ __mz_o_ ] [ cluckj ] [ gnusha ] [ juul ] [ pasky ] [ souljack_ ] 19:07 [ abetusk ] [ Cory ] [ gnusha_ ] [ kanzure ] [ pent ] [ strages ] 19:07 [ adlai ] [ crescend1 ] [ gwillen ] [ kwgwk ] [ pompolic ] [ streety ] 19:07 [ aedla ] [ darsie ] [ gwollon ] [ liead ] [ poppingtonic1] [ sumie-dh ] 19:07 [ andytoshi] [ dequ ] [ heath ] [ maaku ] [ proofoflogic ] [ superkuh ] 19:07 [ archels ] [ docl ] [ helleshin ] [ Madars ] [ Proteus ] [ Taek ] 19:07 [ augur ] [ Douhet ] [ HEx1 ] [ Malvolio ] [ Qfwfq ] [ thundara ] 19:07 [ Aurelius ] [ drewbot ] [ hylleddin ] [ marciogm ] [ redlegion ] [ thxffo ] 19:07 [ balrog ] [ Dumuzi ] [ iaglium ] [ mf1008 ] [ Reventlov ] [ TMA ] 19:07 [ berndj ] [ duper ] [ indiebio ] [ midnightmagic] [ rkos ] [ urchin_ ] 19:07 [ bkero ] [ dustinm ] [ JayDugger ] [ nanotube ] [ rkos_ ] [ vikraman ] 19:07 [ blueskin ] [ EnabrinTain] [ Jenda` ] [ nickjohnson ] [ sachy ] [ WizJin ] 19:07 [ BobaMa ] [ ensign_ ] [ Joshchamp ] [ niekt0 ] [ saturn2 ] [ wrldpcmbl ] 19:07 [ bsm117532] [ ExeciN ] [ jrayhawk ] [ night ] [ saurik ] [ y0no ] 19:07 [ Burninate] [ fenn ] [ jtimon ] [ nmz787_ ] [ sivoais_ ] [ yashgaroth] 19:07 [ c0rw1n ] [ fenn_ ] [ juri_ ] [ nnnn20430 ] [ Solgriffin ] [ yoleaux ] 19:07 [ catern ] [ FourFire ] [ justanotheruser] [ nsh ] [ souljack ] [ yorick ] 19:07 -!- Irssi: ##hplusroadmap: Total of 102 nicks [0 ops, 0 halfops, 0 voices, 102 normal] 19:07 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 19:07 -!- hylleddin1 [~hylleddin@2604:a880:1:20::1056:3001] has joined ##hplusroadmap 19:07 -!- crescendo [~mozart@unaffiliated/crescendo] has joined ##hplusroadmap 19:07 -!- Irssi: Join to ##hplusroadmap was synced in 19 secs 19:07 -!- ExeciN [ExeciN@bnc.stormbit.net] has quit [Ping timeout: 255 seconds] 19:07 -!- souljack [souljack@gateway/shell/xshellz/x-laopbiaqpixlmoqe] has quit [Ping timeout: 255 seconds] 19:07 -!- rkos [rkos@228.ip-176-31-189.eu] has quit [Ping timeout: 255 seconds] 19:07 -!- adlai [~adlai@unaffiliated/adlai] has quit [Ping timeout: 255 seconds] 19:07 -!- crescend1 [~mozart@173.203.100.20] has quit [Ping timeout: 255 seconds] 19:07 -!- gnusha [~gnusha@unaffiliated/kanzure/bot/gnusha] has quit [Ping timeout: 255 seconds] 19:07 -!- fenn [~fenn@unaffiliated/fenn] has quit [Ping timeout: 255 seconds] 19:07 -!- gwillen [~gwillen@unaffiliated/gwillen] has quit [Ping timeout: 255 seconds] 19:07 -!- hylleddin [~hylleddin@2604:a880:1:20::1056:3001] has quit [Ping timeout: 255 seconds] 19:16 -!- gwollon is now known as gwillen 19:18 < kanzure> what page? 19:19 < yashgaroth> 2; p value of 0.7 19:21 < yashgaroth> all the SNPs in APOB taken together might be significant, but it's gonna be a pain to test rs1801703 since the mutation is the default in mice 19:24 < kanzure> hrm got it 19:24 < kanzure> yes that is not a good p value 19:25 -!- ExeciN [ExeciN@bnc.stormbit.net] has joined ##hplusroadmap 20:10 < kanzure> ExeciN: hi 20:12 < kanzure> "CMOS nanoelectrode array for all-electrical intracellular electrophysiological imaging" http://www.nature.com/nnano/journal/vaop/ncurrent/full/nnano.2017.3.html 20:16 < kanzure> "Individual nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to determine protein-RNA interactions" http://biorxiv.org/content/early/2017/02/14/108118 20:18 < kanzure> image of "a single LC neuron labeled with mCherry in a 4 dpf zebrafish" http://www.cell.com/pb-assets/products/pictureshow/confocal-revolution/15.jpg 20:48 -!- poppingtonic1 [~brian@197.182.149.167] has quit [Ping timeout: 258 seconds] 20:54 < nmz787_> yoink on that nanoelectrode paper, thanks! 20:54 < nmz787_> I'll tell work that's basically what I want, plus some micro/nanofluidics 21:55 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 22:01 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 22:18 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 22:46 -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has quit [Quit: Leaving] 22:47 -!- fenn_ is now known as fenn 23:09 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 23:13 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Ping timeout: 240 seconds] 23:21 -!- jtimon [~quassel@245.30.134.37.dynamic.jazztel.es] has quit [Ping timeout: 240 seconds] 23:34 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap --- Log closed Wed Feb 15 00:00:33 2017