--- Log opened Tue Mar 14 00:00:58 2017 02:16 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 02:47 < kanzure> hmph 02:54 -!- bluebear_ [~dluhos@80.95.97.194] has joined ##hplusroadmap 03:02 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 03:13 -!- augur [~augur@2601:645:8300:2b03:44c:dd15:6345:5d4d] has joined ##hplusroadmap 03:23 -!- augur [~augur@2601:645:8300:2b03:44c:dd15:6345:5d4d] has quit [Ping timeout: 246 seconds] 03:28 -!- augur [~augur@2601:645:8300:2b03:20fe:c0c6:e588:f4d1] has joined ##hplusroadmap 03:56 -!- soylentbomb [~k@li44-96.members.linode.com] has quit [Quit: leaving] 04:00 -!- soylentbomb [~k@li44-96.members.linode.com] has joined ##hplusroadmap 04:04 -!- soylentbomb [~k@li44-96.members.linode.com] has quit [Changing host] 04:04 -!- soylentbomb [~k@unaffiliated/soylentbomb] has joined ##hplusroadmap 04:07 -!- soylentbomb [~k@unaffiliated/soylentbomb] has quit [Quit: leaving] 04:07 -!- soylentbomb [~k@unaffiliated/soylentbomb] has joined ##hplusroadmap 04:14 -!- augur [~augur@2601:645:8300:2b03:20fe:c0c6:e588:f4d1] has quit [Remote host closed the connection] 04:43 -!- drewbot [~cinch@ec2-54-81-118-242.compute-1.amazonaws.com] has quit [Remote host closed the connection] 04:43 -!- drewbot [~cinch@ec2-54-211-63-137.compute-1.amazonaws.com] has joined ##hplusroadmap 04:59 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 06:11 < JayDugger> Wasn't the ban count from the FDA going to drop depending on the Trump administration's nominee? 06:36 < kanzure> depends, who got the job? 06:43 -!- Gurkenglas [~Gurkengla@dslb-094-223-131-216.094.223.pools.vodafone-ip.de] has joined ##hplusroadmap 06:58 < streety> I don't think anyone has yet 06:58 < streety> http://www.cnn.com/2017/03/10/politics/scott-gottlieb-donald-trump-fda/index.html Scott Gottlieb seems likely 06:59 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] 06:59 -!- ebowden [~ebowden@1.136.96.136] has joined ##hplusroadmap 06:59 -!- ebowden [~ebowden@1.136.96.136] has quit [Changing host] 06:59 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap 07:22 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 07:24 -!- Adifex [~Adifex@unaffiliated/adifex] has joined ##hplusroadmap 08:53 -!- augur [~augur@c-98-210-157-162.hsd1.ca.comcast.net] has joined ##hplusroadmap 08:54 -!- Guest1117 is now known as abetusk 09:13 < kanzure> .title https://github.com/jeromeetienne/ar.js 09:13 < yoleaux> GitHub - jeromeetienne/AR.js: Efficient Augmented Reality for the Web using ARToolKit - 60fps on mobile! 09:15 -!- c0rw1n [~c0rw1n@138.32-244-81.adsl-dyn.isp.belgacom.be] has quit [Ping timeout: 240 seconds] 09:21 -!- c0rw1n [~c0rw1n@81.244.32.138] has joined ##hplusroadmap 09:27 -!- jtimon [~quassel@70.30.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 09:47 -!- WizJin [WizJin@gateway/shell/fnordserver.eu/x-rvfsmhqnarhowlpr] has joined ##hplusroadmap 09:54 -!- nmz787_i [~ntmccork@134.134.137.71] has quit [Quit: Leaving.] 09:54 -!- Qfwfq [Qfwfq@gateway/shell/fnordserver.eu/x-bgpniwvsmxvagjwl] has joined ##hplusroadmap 10:09 < kanzure> "Anthroposophy is a philosophy founded by Rudolf Steiner that postulates the existence of an objective, intellectually comprehensible spiritual world that is accessible by direct experience through inner development. More specifically, it aims to develop faculties of perceptive imagination, inspiration and intuition through the cultivation of a form of thinking independent of sensory experience." 10:11 -!- TC [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 10:12 -!- TC is now known as Guest1202 10:15 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 246 seconds] 11:04 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-xgjpquvesbfntpyn] has quit [Quit: Connection closed for inactivity] 11:34 -!- bluebear_ [~dluhos@80.95.97.194] has quit [Quit: Leaving.] 11:35 -!- nmz787_i [ntmccork@nat/intel/x-semimdkxjtxtnuli] has joined ##hplusroadmap 11:53 < nsh> -- 11:53 < nsh> External occulters, otherwise known as starshades, have been proposed as a solution to one of the highest priority yet technically vexing problems facing astrophysics - the direct imaging and characterization of terrestrial planets around other stars. New apodization functions, developed over the past few years, now enable starshades of just a few tens of meters diameter to occult central stars so efficiently that the orbiting exoplanets can be 11:53 < nsh> revealed and other high contrast imaging challenges addressed. In this paper an analytic approach to analysis of these apodization functions is presented. It is used to develop a tolerance analysis suitable for use in designing practical starshades. The results provide a mathematical basis for understanding starshades and a quantitative approach to setting tolerances. 11:53 < nsh> -- https://arxiv.org/abs/1106.2127 12:00 < kanzure> .title 12:00 < yoleaux> [1106.2127] Analytic Modeling of Starshades 12:00 < kanzure> i want a hypertelescope 12:04 -!- nmz787_i1 [ntmccork@nat/intel/x-fbvppxcvnahulssp] has joined ##hplusroadmap 12:04 < nmz787_i1> got the plain and GFP e.colis subcultured last night 12:04 < nmz787_i1> instead of waiting for compilers to run, I was waiting for things to pressure cook and then cool down to usable temp 12:05 < nmz787_i1> I didn't do any negative controls though... 12:05 < nmz787_i1> so maybe the ampicillin plates I made are garbage... but probably not 12:05 -!- nmz787_i [ntmccork@nat/intel/x-semimdkxjtxtnuli] has quit [Ping timeout: 260 seconds] 12:06 < nmz787_i1> I now kind of want to make a lab robot 12:07 < kanzure> beep bloop? 12:21 -!- augur [~augur@c-98-210-157-162.hsd1.ca.comcast.net] has quit [Remote host closed the connection] 12:21 < nmz787_i1> not necessarily just a pipet on a gantry either, something that would unscrew jars, open the pressure cooker and insert items (might require fixturing/jigs)... the whole process I did last night... just automated a lot more 12:23 < kanzure> some kinda $400 exome sequencing at 50x coverage https://genos.co/order.html 12:23 < kanzure> er, $500 12:28 < nsh> https://arxiv.org/pdf/1106.2127.pdf 12:28 < nsh> wait, did that one 12:32 < kanzure> sounds like nmz787_i1 has robot-itis. and it's terminal. 12:51 < nsh> -- 12:51 < nsh> The optical properties of plant surfaces are strongly determined by the shape of epidermal cells and by the patterning of the cuticle on top of the cells. Combinations of particular cell shapes with particular nanoscale structures can generate a wide range of optical effects. Perhaps most notably, the development of ordered ridges of cuticle on top of flat petal cells can produce diffraction-grating-like structures. A diffraction grating is one of a 12:51 < nsh> number of mechanisms known to produce 'structural colours', which are more intense and pure than chemical colours and can appear iridescent. We explore the concept that mechanical buckling of the cuticle on the petal epidermis might explain the formation of cuticular ridges, using a theoretical model that accounts for the development of compressive stresses in the cuticle arising from competition between anisotropic expansion of epidermal cells 12:51 < nsh> and isotropic cuticle production. Model predictions rationalize cuticle patterns, including those with long-range order having the potential to generate iridescence, for a range of different flower species. 12:51 < nsh> -- http://rsif.royalsocietypublishing.org/content/10/80/20120847 12:53 < kanzure> .title http://rsif.royalsocietypublishing.org/content/10/80/20120847 12:53 < yoleaux> Buckling as an origin of ordered cuticular patterns in flower petals | Journal of The Royal Society Interface 13:15 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 13:25 -!- urchin_ [~urchin@dsl-89-17-17-162.dsl.h-1.hr] has joined ##hplusroadmap 13:28 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 13:29 -!- CRM114 [~urchin@unaffiliated/urchin] has quit [Ping timeout: 260 seconds] 13:29 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 240 seconds] 14:01 -!- nmz787_i [~ntmccork@134.134.139.78] has joined ##hplusroadmap 14:03 -!- nmz787_i1 [ntmccork@nat/intel/x-fbvppxcvnahulssp] has quit [Remote host closed the connection] 14:30 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Quit: Malvolio] 14:31 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 15:43 -!- nmz787_i [~ntmccork@134.134.139.78] has quit [Quit: Leaving.] 15:57 -!- nmz787_i [~ntmccork@134.134.137.71] has joined ##hplusroadmap 16:12 < nmz787_> hmm, now I need to find some UV light source that I can use to test GFP presence 16:12 < chris_99> would the sun work? 16:12 < yoleaux> 13 Mar 2017 21:59Z chris_99: pretty neat (position determination based on stars), definitely would be a cool app to have 16:33 < nmz787_> chris_99: hmm, maybe if I had a filter, or used my spectrometer 16:34 < chris_99> did you get your electroporator to work though? 16:34 < nmz787_> which reminds me, I got the light source out the last time you asked about me about getting a spectrum of alcohol 16:34 < nmz787_> but since then I left my spectrometer at my friend's lab where we were trying this stuff with the FIB to try detecting x-rays 16:34 < nmz787_> well, got cultures yesteday 16:34 < nmz787_> yesterday* 16:35 < nmz787_> they were supposed to get here Saturday 16:35 < nmz787_> so I subcultured them 16:35 < nmz787_> plain e.coli, and e.coli +GFP+ampicillin_resistance 16:36 < nmz787_> electroporator otherwise is progressing. 16:36 < nmz787_> I have a file that will pulse what I think I want to test with, to begin 16:36 < nmz787_> but adjusting the parameters can cause glitches at some extremes, so I have been working to figure out the whole Timer peripherals better 16:37 < nmz787_> and got a "n"-shot mode working 16:37 < chris_99> aha, fingers crossed then 16:37 < chris_99> how do you control it? 16:37 < chris_99> via usb? 16:38 < nmz787_> which I hope to use to enable two PWM timers, which are out of phase 180 degrees (i.e. 50% of the PWM period), and then after the 'n' pulses are done, fire a shutdown interrupt to turn off the PWM 16:38 < nmz787_> it is flashed with micropython 16:38 < nmz787_> so yeah USB 16:38 < chris_99> oh cool 16:38 < nmz787_> shows up as a serial device and mass-storage drive 16:38 < nmz787_> with the .py files on it 16:39 < nmz787_> I've been using a function that directly programs register memory though 16:39 < nmz787_> since their helper functions for timers don't have all the options exposed 16:40 < chris_99> ahh 16:43 < nmz787_> but yeah got it connected to my oscilloscope on the two low-side pulse channels (the GPIOs), as well as to the high voltage side, through a 40kV-arc-rated 1 gigaohm resistor 16:44 < nmz787_> it's a 10W resistor that was around $35 16:44 < nmz787_> originally bought it for the SEM debug work 16:44 < nmz787_> which I haven't touched in a month or so :/ 16:44 < nmz787_> or maybe weeks 16:45 < nmz787_> since a few weeks before the electroporator board arrived 16:45 < nmz787_> since I built a workbench before that, to host some bio equipment 16:46 < chris_99> cool, what's the workbench made from 16:48 < nmz787_> just some 2x4 wood, a piece of birch plywood for a lower shelf, and a piece of granite countertop on top. the wood is all coated with black RustOleum appliance epoxy 16:49 < chris_99> neat, what's the epoxy for out of interest, to protect the wood? 16:50 < nmz787_> I actually saw that the same place seems to have a matching, wider, piece of counter top granite available... and was thinking I might make another piece that is 2 or 3x wider (since I just have a metal wire shelf there now, which holds stuff, but isn't a solid work surface, smalls things and liquid falls through) 16:50 < nmz787_> it was a spray-paint can application 16:50 < nmz787_> was recommmended to be very hard, hard enough to resist razor blade scratches 16:50 < nmz787_> so I figured I'd give it a try 16:51 < chris_99> ah cool 16:51 < nmz787_> I was concerned with microabrasions, mainly, as far as bio-contamination 16:51 < nmz787_> but went for the granite top for nicer look, and better chemical and fire resistance... than epoxied wood 16:52 < nmz787_> apparently the jet-black granite that is found in labs is the highest-density variety found 16:52 < chris_99> intriguing 16:52 < nmz787_> which I guess is measured, at least in one respect, by how much water it will absorb 16:53 < nmz787_> so I guess my granite has micro-cavities for contamination :/ 16:53 < nmz787_> but is super hard and will resist most chemicals like strong bleach/lysol/alcohol 16:53 < nmz787_> or solvents 16:54 < chris_99> does it also cope with strong acids like fuming nitric etc. out of interest 17:12 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 17:15 < juri_> http://implicitcad.org/ now working properly again. :) 17:28 < streety> I don't think I have ever seen granite lab benches 17:28 < streety> only formica and steel 17:29 < streety> does it actually need anything special for those chemicals? Bleach, lysol abd alcohol you would expect to be fine on any kitchen countertop 17:36 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 260 seconds] 18:27 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] 18:27 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap 18:52 -!- jtimon [~quassel@70.30.134.37.dynamic.jazztel.es] has quit [Ping timeout: 260 seconds] 18:54 < nmz787_i> streety: really? 18:55 < nmz787_i> streety: the typical lab image in my head includes a jet-black stone counter top 18:55 -!- nmz787_i [~ntmccork@134.134.137.71] has quit [Remote host closed the connection] 19:00 < nmz787_> tons of images like this when I search 'school lab' https://s-media-cache-ak0.pinimg.com/originals/33/32/bb/3332bb72cfbab7165a9b4ea46d9013e8.jpg 19:01 -!- thxffo [smdikus@ircbouncehouse.com] has quit [Remote host closed the connection] 19:53 -!- thxffo [smdikus@ircbouncehouse.com] has joined ##hplusroadmap 20:00 < kanzure> do we need to buy you a UV lamp 20:00 < kanzure> what's going on? 20:09 -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:ecf7:bdc1:f677:da63] has joined ##hplusroadmap 20:23 -!- rk[ghost] [~rak@cpe-66-61-32-99.neo.res.rr.com] has quit [Quit: leaving] 20:46 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 20:47 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 246 seconds] 21:56 -!- Gurkenglas [~Gurkengla@dslb-094-223-131-216.094.223.pools.vodafone-ip.de] has quit [Ping timeout: 256 seconds] 22:13 < nmz787_> bought a black light bulb, it worked to confirm the GFP bacteria had a different-enough color to them 22:14 < nmz787_> i want to try my 405nm laser, since that is the peak 22:14 < nmz787_> i have one /somewhere/ 22:15 < nmz787_> also bought a mini fridge, since my gf doesn't like the e.coli in a to-go food container box in the normal food fridge 22:28 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 22:42 -!- juri_ [~juri@205.166.94.194] has quit [Ping timeout: 264 seconds] 22:45 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 260 seconds] 22:58 -!- drewbot_ [~cinch@ec2-54-161-128-103.compute-1.amazonaws.com] has joined ##hplusroadmap 22:58 -!- drewbot [~cinch@ec2-54-211-63-137.compute-1.amazonaws.com] has quit [Remote host closed the connection] 22:59 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 23:14 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 268 seconds] 23:22 -!- ebowden [~ebowden@1.152.96.240] has joined ##hplusroadmap 23:22 -!- ebowden [~ebowden@1.152.96.240] has quit [Changing host] 23:22 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap --- Log closed Wed Mar 15 00:00:04 2017