--- Log opened Sun Mar 19 00:00:02 2017 00:12 -!- drewbot [~cinch@ec2-23-22-169-215.compute-1.amazonaws.com] has quit [Remote host closed the connection] 00:13 -!- drewbot [~cinch@ec2-54-159-190-62.compute-1.amazonaws.com] has joined ##hplusroadmap 00:34 -!- Gurkenglas [~Gurkengla@dslb-188-103-065-154.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 00:59 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 01:04 -!- dequ [christy@taurine.csclub.uwaterloo.ca] has quit [Ping timeout: 256 seconds] 01:05 -!- dequ [christy@taurine.csclub.uwaterloo.ca] has joined ##hplusroadmap 01:16 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Read error: Connection reset by peer] 01:55 -!- augur [~augur@c-98-210-157-162.hsd1.ca.comcast.net] has joined ##hplusroadmap 02:56 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Quit: sleep] 03:08 -!- augur [~augur@c-98-210-157-162.hsd1.ca.comcast.net] has quit [Remote host closed the connection] 03:56 -!- Alpha703 [~Alpha703@2602:306:3021:6a40:7174:357f:3e21:6bc7] has quit [Ping timeout: 264 seconds] 06:15 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 07:32 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 08:03 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 08:08 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 08:09 -!- augur [~augur@2601:645:8300:2b03:21e3:889e:eea7:9db8] has joined ##hplusroadmap 08:11 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 08:13 -!- augur [~augur@2601:645:8300:2b03:21e3:889e:eea7:9db8] has quit [Ping timeout: 246 seconds] 08:19 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 08:29 < kanzure> audio/text alignment tool https://github.com/readbeyond/aeneas 08:37 < JayDugger> Now that's a neat and useful looking tool. 08:44 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 08:51 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 09:31 -!- mr0mx [~mr0mx@187-54-54-201.mganm702.dsl.brasiltelecom.net.br] has joined ##hplusroadmap 09:45 -!- mr0mx [~mr0mx@187-54-54-201.mganm702.dsl.brasiltelecom.net.br] has quit [Quit: Leaving] 09:57 -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:ddd5:56c:1dde:d1a9] has quit [Read error: Connection reset by peer] 10:05 < kanzure> "orco mutagenesis causes loss of antennal lobe glomeruli and impaired social behavior in ants" http://biorxiv.org/content/early/2017/02/28/112532 10:05 < kanzure> "We find that orco mutants exhibit severe deficiencies in social behavior and fitness, suggesting that they are unable to perceive pheromones. Surprisingly, unlike in Drosophila melanogaster, orco mutant ants also lack most of the approximately 500 antennal lobe glomeruli found in wild-types. These results illustrate that ORs are essential for ant social organization, and raise the possibility... 10:05 < kanzure> ... that, similar to mammals, receptor function is required for the development and/or maintenance of the highly complex olfactory processing areas in the ant brain." 10:41 < kanzure> mutant ants. 11:40 -!- Gurkenglas [~Gurkengla@dslb-188-103-065-154.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 260 seconds] 11:56 -!- Gurkenglas [~Gurkengla@dslb-188-103-065-154.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 12:00 -!- Alpha703 [~Alpha703@2602:306:3021:6a40:7174:357f:3e21:6bc7] has joined ##hplusroadmap 12:05 -!- augur [~augur@c-98-210-157-162.hsd1.ca.comcast.net] has joined ##hplusroadmap 12:24 < kanzure> "The multi-disciplinary consortium spans 7 institutions. ReadCoor's team is led by Richard Terry, Samuel Inverso, PhD, Brian Turczyk, PhD, and Allison Martin. In addition to consortium Principal Investigator George Church, Ph.D., the Harvard Wyss Institute's effort is led by co-Investigator Richie Kohman, Ph.D. Complementing the Wyss team, are co-Principal Investigators Anthony Zador, Ph.D., A... 12:24 < kanzure> ...lexei Koulakov, Ph.D., and Alexander Vaughan, Ph.D., at Cold Spring Harbor Laboratory. Adam Marblestone, Ph.D., and Liam Paninski, Ph.D. are co-Investigator at MIT and co-Principal Investigator at Columbia University, respectively, and the scientists are partnering with another MICrONS team led by Tai Sing Lee, Ph.D. and Sandra Khulman, Ph.D. as co-Principal Investigators at Carnegie Mellon Un... 12:24 < kanzure> ...iversity." 12:54 < nmz787_> kanzure: here's a repeatable pipette blowtorch (that uses electricity for heat, rather than hydrocarbons): http://www.ebay.com/itm/Flaming-brown-Micropipette-Puller-P-97-/322440815367?hash=item4b12f86307:g:w5oAAOSw44BYF2lj 12:56 < nmz787_> kanzure: btw I have access to one of those, and I think it is set on something like a 1 micron end-diameter for its normal program (it has space for a few 'recipes') 13:03 -!- augur [~augur@c-98-210-157-162.hsd1.ca.comcast.net] has quit [Remote host closed the connection] 13:03 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 13:05 < nmz787_> (same lab that has the FIB, which they use to clean up ends of pipettes, sometimes... so they're a standard dimension) 13:09 < kanzure> what? FIB cleaning of pipette tips? how does that work. 13:12 < nmz787_> just like that 13:13 < nmz787_> sputter on some carbon to the tip, so it conducts well enough to bleed down charge 13:13 < nmz787_> then just FIB away 13:13 < kanzure> but what about inside the pipette tip 13:13 < nmz787_> the pipette puller begins with a capillary tube 13:14 < nmz787_> and physics of the drawing process mean the inner diameter and outer diameter ratio scales similarly or something 13:14 < nmz787_> the FIB is mostly just to chop off the end at a specific diameter (tip opening) 13:15 < nmz787_> single cut 13:15 < kanzure> no we were talking about cleaning 13:15 < nmz787_> (well single high-power cut, then lower power deburring) 13:15 < kanzure> oh you mean clean the manufacturing results, not... cleaning. 13:16 < nmz787_> oh, yeah 13:16 < nmz787_> in case the pipette puller made the tip too small, or not consistent 13:16 < kanzure> i dunno maybe like n-axis pipette rotation to get the inner part in the path of the FIB beam, then just shoot ions at everything to clean the tube, problem solved? 13:17 < nmz787_> why not just use strong bleach/caustic? 13:17 < kanzure> right 13:18 < nmz787_> but yeah, e-beam could desorb/vaporize things too 13:18 < nmz787_> https://sites.google.com/site/biocuriousquantumbiology/home/intro-to-quantum-bio-project 13:18 < nmz787_> the attached doc has relevant equations for suface-plasmon resonance detector setup 13:20 < nmz787_> basically it looks like SPR 'appears' like a change in refractive index... i.e. analysis substrate reflects a laser at a given angle, analysis substrate + other molecule -> changes laser reflection angle 13:22 < nmz787_> which makes sense if you think about molecules as dynamic antennas/filters 13:30 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 13:32 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 13:35 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 13:36 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 13:36 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 13:46 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 13:50 -!- m4l3z [~m4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap 14:38 -!- m4l3z [~m4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Quit: Leaving] 14:40 -!- TC [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 14:40 -!- TC is now known as Guest4671 14:43 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 246 seconds] 17:14 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-issswzzvvjpmuutg] has quit [Quit: Connection closed for inactivity] 17:21 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:dcf7:edb6:b6d2:b2a4] has joined ##hplusroadmap 18:42 -!- Gurkenglas [~Gurkengla@dslb-188-103-065-154.188.103.pools.vodafone-ip.de] has quit [Read error: Connection reset by peer] 19:18 -!- Gurkenglas [~Gurkengla@dslb-188-103-065-154.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 19:47 < kanzure> bloop 19:48 < nmz787_> https://academic.oup.com/nar/article/2990259/Measurements-of-translation-initiation-from-all-64 19:48 < nmz787_> .title 19:48 < yoleaux> Measurements of translation initiation from all 64 codons in E. coli | Nucleic Acids Research | Oxford Academic 19:48 < nmz787_> see fig 2 19:49 < nmz787_> https://www.nist.gov/news-events/news/2017/02/start-codons-dna-and-rna-may-be-more-numerous-previously-thought 19:49 < nmz787_> the image there with streaked bacteria is nice too 19:50 -!- Gurkenglas [~Gurkengla@dslb-188-103-065-154.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 260 seconds] 22:17 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 264 seconds] 22:43 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Quit: sleep] 22:48 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 260 seconds] 22:59 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:dcf7:edb6:b6d2:b2a4] has quit [Quit: Leaving] 23:37 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 23:37 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has left ##hplusroadmap [] 23:50 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap --- Log closed Mon Mar 20 00:00:03 2017